CN204550580U - A kind of miniature tangential flow filtration simulator - Google Patents
A kind of miniature tangential flow filtration simulator Download PDFInfo
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- CN204550580U CN204550580U CN201520138512.4U CN201520138512U CN204550580U CN 204550580 U CN204550580 U CN 204550580U CN 201520138512 U CN201520138512 U CN 201520138512U CN 204550580 U CN204550580 U CN 204550580U
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Abstract
A kind of miniature tangential flow filtration simulator, it is characterized in that: it comprises head tank (1), stock liquid in head tank (1) pumps into filtration unit (3) by the peristaltic pump (2) on head tank (1) delivery conduit, described filtration unit (3) output terminal is connected with back pressure valve (4), back pressure valve (4) output terminal connects back head tank (1), described peristaltic pump (2) and filtration unit (3) connecting pipeline are equipped with the first pressure transmitter (5), described filtration unit (3) and back pressure valve (4) connecting pipeline are equipped with the second sensor (6), the utility model utilizes miniature tangential flow filtration device, it is made to have at least two atmospheric sealing intensities, between runner, seepage is there is not along flow passage in fluid in filtration unit, in conjunction with miniature head tank, achieve the microminiaturization of simulator, from and high value biotechnological formulation low cost, high speed, high-throughout downstream process fast Development may be realized, saved cost of development, shortened the construction cycle.
Description
Technical field
The utility model belongs to bio-pharmaceuticals experiment equipment technical field, specifically just relate to miniature tangential flow filtration simulator in a kind of designative species, reduce the volume of simulator, improve simulation test precision, make high-throughput large batch of experiment become possibility.
Background technology
Along with the development of science and technology, take monoclonal antibody as the main products of medical protein as designative species of representative, at medical market in occupation of share significantly.In whole production technique, the cost of downstream separation purifying process occupies the 50%-80% of total cost, and wherein tangential flow membrane separation technique is separated at cell-crude protein and is separated with protein-protein and becomes main separation means in protein soln replacing etc.
In recent years, monoclonal antibody industry was rapid and develop healthy and strongly, once cell culture process is successfully established, under the pressure of market and imitated medicine specific program, downstream separation purifying parameter needs optimised within some months and determines.In addition, because animal source antibody itself is expensive, early stage, the high cost of optimization experiment also allowed main market players assume responsibility for larger risk, once test unsuccessful, can bring about great losses.Therefore, setting up and improve a set of Novel filtration technology platform, for optimizing fast and economically downstream film separation and purification operating parameters, becoming technical barrier urgently to be resolved hurrily at present.
In order to reduce the cost of monoclonal antibody industry downstream separation purifying experimental formula, at present worldwide, scientific research institution is also starting to note the microminiaturization of tangential flow systems to simulate nearly ten years, this traditional its research direction of microminiaturized simulator mainly utilizes stirred vessel to simulate tangential flow situation, does not directly reduce tangential flow systems.The advantage of this mode is the controllable operating of shearing force and the treatment capacity at larger unit membrane area.But this mode also has obvious shortcoming, first be owing to having installed miniature variable speed motor, increase with making the significant cost of filtering element, make it be unsuitable for batch production and large-scale application, also make the stopping property of filtering element reduce due to the insertion of rotating shaft simultaneously; In addition, the shearing situation produced by stirring may not necessarily present shearing force distribution situation during common tangential flow filtration accurately, makes experimental data distortion.
Utility model content
The purpose of this utility model is for the miniature tangential flow filtration simulator of existing protein separation test, sealing property is not good, the technological deficiency of shearing force distribution situation when completely precisely cannot simulate common tangential flow filtration, there is provided a kind of cell, cell submicron particle and protein separation formula test miniature tangential flow filtration simulator, reduce the cost of whole device, improve verity and the accuracy of the simulation in laboratory.
Technical scheme
In order to realize above-mentioned technical purpose, the present invention designs a kind of miniature tangential flow filtration simulator, it is characterized in that: it comprises head tank, stock liquid in head tank pumps into filtration unit by the peristaltic pump on head tank delivery conduit, described filtration unit output terminal is connected with back pressure valve, back pressure valve output terminal connects back head tank, and described peristaltic pump and filtration unit connecting pipeline are equipped with the first pressure transmitter, and described filtration unit and back pressure valve connecting pipeline are equipped with the second sensor;
Described head tank comprises tank body, and tank body is arranged on tank base, the oral area sealing of tank cover of tank body, described tank body top is provided with liquid-inlet pipe and bushing pipe and extraneous UNICOM, tank body lower part is provided with drain pipe and extraneous UNICOM, and described liquid-inlet pipe is connected on back pressure valve, and described drain pipe is connected on peristaltic pump;
Described filtration unit comprises circulation box body and lower circulation box body, upper circulation box body and the laminating of lower circulation box body are fixed together, filtering membrane is provided with between described upper circulation box body and lower circulation box body binding face, described upper circulation box body lower surface is provided with upper runner groove, lower circulation cartridge upper surface is provided with lower flow channel groove, lower flow channel groove and upper runner groove correspondence, the two ends of described upper runner groove are equipped with charging respectively and input bushing pipe are housed and export bushing pipe, input bushing pipe and export bushing pipe to be connected respectively on peristaltic pump and back pressure valve; Described lower flow channel groove is connected with discharging bushing pipe and extraneous UNICOM;
Between described upper circulation box body and lower circulation box body, sealing-ring is housed to seal.
Further, described discharging bushing pipe is through lower circulation box body and extraneous UNICOM.
Further, described sealing-ring be contained in circulation box body lower surface mounting groove in or lower circulation box body is shown
In the mounting groove in face.
Further, described head tank is also connected with feed supplement tank, described feed supplement tank is connected with bushing pipe.
Further, the flow channel shape of described lower flow channel groove and upper runner groove is volution or rectangle or annular.
Further, described peristaltic pump is multi-headed peristaltic pump, and pump head quantity is 1 ~ 6, and multiple stage peristaltic pump can parallelly use.
Beneficial effect
The miniature tangential flow filtration simulator of one provided by the utility model, utilize miniature tangential flow filtration device, it is made to have at least two atmospheric sealing intensities, between runner, seepage is there is not along flow passage in fluid in filtration unit, in conjunction with miniature head tank, achieve the microminiaturization of simulator, from and high value biotechnological formulation low cost, high speed, high-throughout downstream process fast Development may be realized, save cost of development, shorten the construction cycle.
Accompanying drawing explanation
Accompanying drawing 1 is the annexation schematic diagram of the utility model first embodiment.
Accompanying drawing 2 is the utility model first embodiment Raw jar structure schematic diagram.
Accompanying drawing 3 is filter apparatus configuration schematic diagram in the utility model first embodiment.
Accompanying drawing 4 is filtration unit vertical views in the utility model first embodiment.
Accompanying drawing 5 is upper circulation box body upward views in the utility model first embodiment.
Accompanying drawing 6 is lower circulation box body structure schematic diagram in the utility model first embodiment.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described further.
Embodiment 1, as shown in Figure 2, described head tank 1 comprises tank body 101, tank body 101 is arranged on tank base 102, the oral area cover 103 of tank body 101 seals, and described tank body 101 top is provided with liquid-inlet pipe 104 and bushing pipe 105 and extraneous UNICOM, and tank body 101 bottom is provided with drain pipe 106 and extraneous UNICOM, described liquid-inlet pipe 104 is connected on back pressure valve 4, and described drain pipe 106 is connected on peristaltic pump 2.
As accompanying drawing 3, shown in 4 and 5, described filtration unit 3 comprises circulation box body 301 and lower circulation box body 302, upper circulation box body 301 and the laminating of lower circulation box body 302 are fixed together, filtering membrane 303 is provided with between described upper circulation box body 301 and lower circulation box body 302 binding face, described upper circulation box body 301 lower surface is provided with upper runner groove 301a, lower circulation box body 302 upper surface is provided with lower flow channel groove 302a, lower flow channel groove 302a and upper runner groove 301a is corresponding, the two ends of described upper runner groove 301a are equipped with charging respectively and input bushing pipe 304 are housed and export bushing pipe 305, input bushing pipe 304 and output bushing pipe 305 are connected respectively on peristaltic pump 2 and back pressure valve 4, described lower flow channel groove 302a is connected with discharging bushing pipe 306 and extraneous UNICOM,
Between described upper circulation box body 301 and lower circulation box body 302, sealing-ring 307 is housed to seal.
Described discharging bushing pipe 306 is through lower circulation box body 302 and extraneous UNICOM.
Described sealing-ring 307 is contained in the mounting groove of circulation box body 301 lower surface.
Described head tank 1 is also connected with feed supplement tank 7, and described feed supplement tank 7 is connected with bushing pipe 105.
The flow channel shape of described lower flow channel groove 302a and upper runner groove 301a is volution.
Described peristaltic pump 2 is multi-headed peristaltic pump, and pump head quantity is 1 ~ 6.
Embodiment 2
The flow channel shape of described lower flow channel 302a and upper runner groove 301a is rectangle.
Embodiment 3
The flow channel shape of described lower flow channel 302a and upper runner groove 301a is annular.
Embodiment 4
Described sealing-ring 307 is contained in the mounting groove of lower circulation box body 302 upper surface.
In the present invention, stock liquid is pumped into filtration unit 3 from the peristaltic pump 2 former delivery conduit, filters through filtering membrane 303, after from lower circulation box body 302 lower flow channel groove 302a flow out through discharging bushing pipe 306, detect and obtain the performance of test substance.Whole device makes it have at least two atmospheric sealing intensities, between runner, seepage is there is not along flow passage in fluid in filtration unit, in conjunction with miniature head tank, achieve the microminiaturization of simulator, from and high value biotechnological formulation low cost, high speed, high-throughout downstream process fast Development may be realized, save cost of development, shorten the construction cycle, adopt the large-scale filter plant of filtration unit provided by the invention and laboratory to carry out test comparison test parameter and test-results as follows:
Table 1: operational condition
Table 2: under identical transmembrane pressure, laboratory scale strainer and on a small scale strainer ultrafiltered proteins matter transmitance compare
| Transmembrane pressure (bar) | Laboratory scale strainer | Strainer on a small scale |
| 0.3 (laboratory)/0.1 (on a small scale) | 35％ | 38.3％ |
| 0.9 (laboratory)/0.3 (on a small scale) | 30.5％ | 33.2％ |
| 1.5 (laboratory)/0.5 (on a small scale) | 29.5％ | 32.3％ |
Note: carry out the lysozyme soln that filted chroma is 0.6g/L with the film that molecular weight cut-off is 30KDa.
In transmitance=filtrate albumen concentration/phegma in the concentration of albumen.
Compare by analysis, both test results is similar to, therefore present invention achieves the microminiaturization of simulator, from and high value biotechnological formulation low cost, high speed, high-throughout downstream process fast Development may be realized, save cost of development, shorten the construction cycle.
Claims (6)
1. a miniature tangential flow filtration simulator, it is characterized in that: it comprises head tank (1), stock liquid in head tank (1) pumps into filtration unit (3) by the peristaltic pump (2) on head tank (1) delivery conduit, described filtration unit (3) output terminal is connected with back pressure valve (4), back pressure valve (4) output terminal connects back head tank (1), described peristaltic pump (2) and filtration unit (3) connecting pipeline are equipped with the first pressure transmitter (5), described filtration unit (3) and back pressure valve (4) connecting pipeline are equipped with the second sensor (6),
Described head tank (1) comprises tank body (101), tank body (101) is arranged on tank base (102), the oral area of tank body (101) seals with cover (103), described tank body (101) top is provided with liquid-inlet pipe (104) and bushing pipe (105) and extraneous UNICOM, tank body (101) bottom is provided with drain pipe (106) and extraneous UNICOM, described liquid-inlet pipe (104) is connected on back pressure valve (4), and described drain pipe (106) is connected on peristaltic pump (2);
Described filtration unit (3) comprises circulation box body (301) and lower circulation box body (302), upper circulation box body (301) and lower circulation box body (302) laminating are fixed together, filtering membrane (303) is provided with between described upper circulation box body (301) and lower circulation box body (302) binding face, described upper circulation box body (301) lower surface is provided with upper runner groove (301a), lower circulation box body (302) upper surface is provided with lower flow channel groove (302a), lower flow channel groove (302a) and upper runner groove (301a) correspondence, the two ends of described upper runner groove (301a) are equipped with charging respectively and input bushing pipe (304) are housed and export bushing pipe (305), input bushing pipe (304) and output bushing pipe (305) are connected respectively on peristaltic pump (2) and back pressure valve (4), described lower flow channel groove (302a) is connected with discharging bushing pipe (306) and extraneous UNICOM,
Between described upper circulation box body (301) and lower circulation box body (302), sealing-ring (307) is housed to seal.
2. a kind of miniature tangential flow filtration simulator as claimed in claim 1, is characterized in that: described discharging bushing pipe (306) is through lower circulation box body (302) and extraneous UNICOM.
3. a kind of miniature tangential flow filtration simulator as claimed in claim 1, is characterized in that: in the mounting groove that described sealing-ring (307) is contained in circulation box body (301) lower surface or in the mounting groove of lower circulation box body (302) upper surface.
4. a kind of miniature tangential flow filtration simulator as claimed in claim 1, is characterized in that: described head tank (1) is also connected with feed supplement tank (7), and described feed supplement tank (7) is connected with bushing pipe (105).
5. a kind of miniature tangential flow filtration simulator as claimed in claim 1, is characterized in that: the flow channel shape of described lower flow channel groove (302a) and upper runner groove (301a) is volution or rectangle or annular.
6. a kind of miniature tangential flow filtration simulator as claimed in claim 1, it is characterized in that: described peristaltic pump (2) is multi-headed peristaltic pump, pump head quantity is 1 ~ 6.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520138512.4U CN204550580U (en) | 2015-03-10 | 2015-03-10 | A kind of miniature tangential flow filtration simulator |
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| CN201520138512.4U CN204550580U (en) | 2015-03-10 | 2015-03-10 | A kind of miniature tangential flow filtration simulator |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673666A (en) * | 2015-03-10 | 2015-06-03 | 华东理工大学 | Micro tangential flow filtration simulation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673666A (en) * | 2015-03-10 | 2015-06-03 | 华东理工大学 | Micro tangential flow filtration simulation device |
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