CN112321672A - Method for extracting bioactive peptide from embryo eggs - Google Patents
Method for extracting bioactive peptide from embryo eggs Download PDFInfo
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- CN112321672A CN112321672A CN202011468579.6A CN202011468579A CN112321672A CN 112321672 A CN112321672 A CN 112321672A CN 202011468579 A CN202011468579 A CN 202011468579A CN 112321672 A CN112321672 A CN 112321672A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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Abstract
The invention discloses a method for extracting bioactive peptide from an embryo egg, which comprises a collecting box, a culture box and an upper cover, wherein the collecting box is provided with two standing grooves which are distributed up and down; an operator puts the shelled eggs into a culture space divided by a first filter plate and a separation plate in an incubator, active peptides and water molecules in egg white can permeate through the first ultrafiltration membrane on the first filter plate and a second ultrafiltration membrane on the second filter plate to flow out, the size of a filter hole of a third ultrafiltration membrane on an active peptide collecting box is zero point four to five nanometers, only water molecules can be allowed to pass, the water molecules in the active peptide collecting box can pass through the third ultrafiltration membrane and fall into a wastewater collecting box, the water molecules and the active peptides are separated, and therefore the active peptides are extracted.
Description
Technical Field
The invention relates to the technical field of extraction of bioactive peptides, in particular to a method for extracting bioactive peptides from embryo eggs.
Background
The peptide is a compound formed by connecting two or more amino acids by peptide bonds, and the active peptide plays a key role in the processes of growth and development, metabolism, diseases, aging and death of human beings. Active peptide is the most important active substance in human body, and with the development of science and technology, people find that a large amount of active peptide in embryo eggs can be utilized by people. However, when extracting the active peptides from the embryonated eggs, various complicated processes are required to remove the proteins from the embryonated eggs, and various reagents are required to extract the active peptides, which results in complicated processes and high cost.
Disclosure of Invention
The invention aims to provide a method for extracting bioactive peptide from an embryo egg, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the extraction method of the bioactive peptide in the embryo eggs comprises a collection box, an incubator and an upper cover, wherein the collection box is provided with two vertical placement grooves, sliding grooves are formed in two sides of the top of each placement groove, the top of the collection box is provided with a hollow groove, a bioactive peptide collection box is arranged in the upper placement groove, a wastewater collection box is arranged in the lower placement groove, the incubator is connected with the collection box in the hollow groove in a clamping manner, the incubator is detachably connected with the collection box, a plurality of first filter plates which are uniformly distributed are fixedly connected in the incubator, every two first filter plates are in one group, one side, opposite to each other, of each group of two first filter plates is fixedly connected with a first ultrafiltration membrane, a plurality of uniformly distributed partition plates are fixedly connected between each group of first filter plates, and the upper cover is positioned at the top of the incubator, just the upper cover with the connection can be dismantled to the collecting box, the upper cover top is equipped with a plurality of evenly distributed's transfer line, just the position and every group of transfer line first filter plate are corresponding, transfer line bottom fixed connection is equipped with a plurality of evenly distributed's small-size solenoid valve, small-size solenoid valve and outside PLC controller signal connection, the transfer line with the small-size solenoid valve input is connected, transfer line one end is connected with the infusion hose, the infusion hose is kept away from the one end and the outside water pump of transfer line are connected.
As a still further scheme of the invention: the active peptide collecting box comprises an active peptide collecting box body, and is characterized in that first sliding plates are fixedly connected to two sides of the top of the active peptide collecting box body, the first sliding plates are arranged in sliding grooves and are connected with the collecting box in a sliding mode, third filter plates are fixedly connected to the bottom of the active peptide collecting box body, third ultrafiltration membranes are fixedly connected to the tops of the third filter plates, second sliding plates are fixedly connected to two sides of the top of the wastewater collecting box body, and the second sliding plates are arranged in the sliding grooves and.
As a still further scheme of the invention: the incubator outer wall is fixedly connected with a mounting plate, a mounting bolt is arranged on the mounting plate, and the mounting plate is fixed with the collecting box through the mounting bolt in a threaded connection mode.
As a still further scheme of the invention: fixedly connected with temperature sensor on the division board, temperature sensor and outside PLC controller signal connection, fixed connection is in humidity transducer on the division board, humidity transducer and outside PLC controller signal connection, incubator bottom fixedly connected with second filter plate, second filter plate top fixedly connected with second milipore filter.
As a still further scheme of the invention: the incubator outer wall fixedly connected with vertical data plate, incubator outer wall fixedly connected with horizontal data plate, a plurality of evenly distributed's collecting vat has been seted up in the incubator, just the collecting vat is located every group first filter plate both sides.
As a still further scheme of the invention: the collecting box is characterized in that connecting columns are fixedly connected to four corners of the upper cover, one end, far away from the upper cover, of each connecting column is fixedly connected with a connecting plate, a connecting bolt is arranged on each connecting plate, and each connecting plate is fixed with the collecting box in a threaded connection mode through the corresponding connecting bolt.
As a still further scheme of the invention: the output end of the small electromagnetic valve is fixedly connected with a supply pipe, and one end, far away from the small electromagnetic valve, of the supply pipe extends into the incubator.
As a still further scheme of the invention: one side of the upper cover, which is far away from the infusion tube, is fixedly connected with a plurality of hatching lamps which are uniformly distributed, and the hatching lamps are in signal connection with an external PLC (programmable logic controller).
As a further scheme of the invention, the method for extracting the bioactive peptide from the embryo eggs comprises the following steps:
a: when the device is used, the device is firstly placed in a sterile environment, then the active peptide collecting box and the waste water collecting box are respectively inserted into the placing grooves, the active peptide collecting box is inserted into the placing groove above, the waste water collecting box is inserted into the placing groove below, the incubator is connected with the collecting box, an operator peels the fertilized eggs and the eggshells, the peeled eggs are placed into the culture spaces separated by the first filter plate and the separating plate in the incubator, and the upper cover can be covered after the operation is completed.
B: after the step A is finished, because the size of water molecules is zero-point four nanometers smaller than that of active peptides, the size of proteins is one to one hundred nanometers larger than that of the active peptides, and the sizes of filter holes of the first ultrafiltration membrane and the second ultrafiltration membrane are smaller than one nanometer, the active peptides and the water molecules in the egg white can flow out through the first ultrafiltration membrane on the first filter plate and the second ultrafiltration membrane on the second filter plate, the number of supply pipes on the upper cover corresponds to the number of each divided culture space, the supply pipes are positioned above each divided culture space, the hatching lamps correspond to each culture space, the hatching lamps are positioned above each divided culture space, the temperature and the humidity in each culture space can be sensed through the temperature sensor and the humidity sensor on the partition plate, when the temperature is higher or lower, the temperature sensor will send a signal to outside PLC controller, thereby adjust the heat that gives off of hatching lamp, because the hydrone also can pass first milipore filter and second milipore filter, so when the egg moisture in the culture space reduces, the humidity sensor just can sense, send a signal to outside PLC controller immediately, will open the small-size solenoid valve that corresponds with the culture space of lack of water, restart outside water pump, thereby in the culture space of lack of water is carried to nutrient solution along transfer line and supply pipe, thereby guarantee that the egg in the culture space can healthy growth.
C: and C, after the step B is completed, the active peptide and the water molecules which pass through the first ultrafiltration membrane and the second ultrafiltration membrane are collected by the active peptide collecting box, the size of a filter hole of a third ultrafiltration membrane on the active peptide collecting box is zero four-five nanometers, only the water molecules can be allowed to pass through, the water molecules in the active peptide collecting box can fall into the wastewater collecting box, so that the water molecules and the active peptide are separated, when the embryonated eggs develop in the culture space for ten days to thirteen days, the chicken inside can be gradually formed, the egg white can be consumed, and at this time, the embryonated eggs in the culture space can be taken out to be used for extracting the active peptide in the egg yolk in the next step.
Compared with the prior art, the invention has the beneficial effects that:
1. when the device is used, the device is placed in a sterile environment, an operator peels the fertilized eggs and the egg shells, the peeled eggs are placed in a culture space which is divided by a first filter plate and a partition plate in an incubator, active peptides and water molecules in the egg shells flow out through the first ultrafiltration membrane on the first filter plate and the second ultrafiltration membrane on the second filter plate, and are collected by an active peptide collecting box, the size of a third ultrafiltration membrane filter hole on the active peptide collecting box is zero point four-five nanometers, only water molecules can be allowed to pass through, the water molecules in the active peptide collecting box can fall into a waste water collecting box, and the water molecules and the active peptides are separated, so that the active peptides are extracted, the process is simple, and the cost is low.
Drawings
FIG. 1 is a schematic diagram of a method for extracting bioactive peptides from an embryo egg;
FIG. 2 is a schematic view of the structure of a collection box in a method for extracting bioactive peptides from an embryo egg;
FIG. 3 is a schematic diagram of the structure of a bioactive peptide collection box in the method for extracting bioactive peptide from an embryo egg;
FIG. 4 is a schematic view of a wastewater collection tank in a method for extracting bioactive peptides from embryonated eggs;
FIG. 5 is a schematic view of the structure of a collection box and an incubator in a method for extracting bioactive peptides from an embryo egg;
FIG. 6 is a schematic structural diagram of a culture box and an upper cover in the method for extracting bioactive peptides from embryo eggs;
FIG. 7 is a flow chart of a method for extracting bioactive peptides from an embryo egg.
In the figure: 1. a collection box; 11. a placement groove; 111. a chute; 12. an empty groove; 13. a bioactive peptide collecting box; 131. a first slide plate; 132. a third filter plate; 133. a third ultrafiltration membrane; 14. a wastewater collection tank; 141. a second slide plate; 2. an incubator; 21. mounting a plate; 211. installing a bolt; 22. a first filter plate; 221. a first ultrafiltration membrane; 23. a partition plate; 24. a temperature sensor; 25. a humidity sensor; 26. a second filter plate; 261. a second ultrafiltration membrane; 27. a longitudinal nameplate; 28. a transverse nameplate; 29. collecting tank; 3. an upper cover; 31. connecting columns; 311. a connecting plate; 312. a connecting bolt; 32. a transfusion tube; 33. a small-sized solenoid valve; 331. a supply pipe; 34. a flexible infusion tube; 35. a hatching lamp.
Detailed Description
Referring to fig. 1-7, in the embodiment of the invention, a method for extracting bioactive peptides from an embryo egg comprises a collection box (1), an incubator (2) and an upper cover (3), wherein the collection box (1) is provided with two vertically distributed standing grooves (11), sliding grooves (111) are formed in two sides of the top of each standing groove (11), a hollow groove (12) is formed in the top of the collection box (1), a bioactive peptide collection box (13) is arranged in the standing groove (11) positioned above the collection box (1), a wastewater collection box (14) is arranged in the standing groove (11) positioned below the collection box, the incubator (2) is connected with the collection box (1) in a clamping manner in the hollow groove (12), the incubator (2) is detachably connected with the collection box (1), a plurality of uniformly distributed first filter plates (22) are fixedly connected in the incubator (2), every two first filter plates (22) form a group, a first ultrafiltration membrane (221) is fixedly connected to one side, which is opposite to the two first, division board (23) of fixedly connected with a plurality of evenly distributed between every first filter plate of group (22), upper cover (3) are located incubator (2) top, and upper cover (3) can be dismantled with collecting box (1) and be connected, upper cover (3) top is equipped with a plurality of evenly distributed's transfer line (32), and the position of transfer line (32) is corresponding with first filter plate of every group (22), transfer line (32) bottom fixed connection is equipped with a plurality of evenly distributed's small-size solenoid valve (33), small-size solenoid valve (33) and outside PLC controller signal connection, transfer line (32) are connected with small-size solenoid valve (33) input, transfer line (32) one end is connected with infusion hose (34), the one end that transfer line (32) were kept away from in infusion hose (34) is connected with outside water pump.
In fig. 1, 2, 3 and 4: the collecting box (1) is provided with two vertically distributed placing grooves (11), sliding grooves (111) are formed in two sides of the top of each placing groove (11), the top of each collecting box (1) is provided with a hollow groove (12), an active peptide collecting box (13) is arranged in the placing groove (11) positioned above, a wastewater collecting box (14) is arranged in the placing groove (11) positioned below, two sides of the top of each active peptide collecting box (13) are fixedly connected with first sliding plates (131), the first sliding plates (131) are slidably connected with the collecting boxes (1) in the sliding grooves (111), the bottom of each active peptide collecting box (13) is fixedly connected with third filter plates (132), the top of each third filter plate (132) is fixedly connected with a third ultrafiltration membrane (133), two sides of the top of each wastewater collecting box (14) are fixedly connected with second sliding plates (141), and the second sliding plates (141), when in use, the active peptide collecting box (13) and the waste water collecting box (14) are respectively inserted into the placing groove (11), the active peptide collecting box (13) is inserted into the placing groove (11) above, the waste water collecting box (14) is inserted into the placing groove (11) below, the active peptide and water molecules which pass through the first ultrafiltration membrane (221) and the second ultrafiltration membrane (261) are collected by the active peptide collecting box (13), and the size of the filtration pore of the third ultrafiltration membrane (133) on the active peptide collection box (13) is zero four-five nanometers, only water molecules can pass through, the water molecules in the active peptide collection box (13) can fall into the wastewater collection box (14), thereby separating the water molecules and the active peptide, then the active peptide collecting box (13) and the waste water collecting box (14) are extracted, thereby respectively treating the collected active peptide and waste water.
In fig. 1, 5, 6 and 7: the incubator (2) is connected with the collecting box (1) in the empty groove (12) in a clamping manner, the incubator (2) is detachably connected with the collecting box (1), the outer wall of the incubator (2) is fixedly connected with a mounting plate (21), the mounting plate (21) is provided with a mounting bolt (211), the mounting plate (21) is fixedly connected with the collecting box (1) through the mounting bolt (211) in a threaded manner, the incubator (2) is placed in the empty groove (12) during installation, the mounting bolt (211) penetrates through the mounting plate (21) and is screwed into the collecting box (1), the mounting bolt (211) is screwed out of the collecting box (1) during disassembly, the incubator (2) is taken out, so that the installation and disassembly between the collecting box (1) and the incubator (2) are realized, a plurality of first filter plates (22) which are uniformly distributed are fixedly connected in the incubator (2), and every two first filter plates (22) form a group, one side of each group of two first filter plates (22) opposite to each other is fixedly connected with a first ultrafiltration membrane (221), a plurality of uniformly distributed partition plates (23) are fixedly connected between each group of first filter plates (22), temperature sensors (24) are fixedly connected onto the partition plates (23), the temperature sensors (24) are in signal connection with an external PLC (programmable logic controller), the partition plates (23) are fixedly connected onto humidity sensors (25), the humidity sensors (25) are in signal connection with the external PLC, the bottom of the incubator (2) is fixedly connected with a second filter plate (26), the top of the second filter plate (26) is fixedly connected with a second ultrafiltration membrane (261), the outer wall of the incubator (2) is fixedly connected with a longitudinal nameplate (27), the outer wall of the incubator (2) is fixedly connected with a transverse collecting tank (28), a plurality of uniformly distributed collecting tanks (29) are arranged in the incubator (2), and the collecting tanks (29) are positioned at two sides of each group of, the upper cover (3) is positioned at the top of the incubator (2), the upper cover (3) is detachably connected with the collection box (1), connecting columns (31) are fixedly connected to four corners of the upper cover (3), one end, far away from the upper cover (3), of each connecting column (31) is fixedly connected with a connecting plate (311), a connecting bolt (312) is arranged on each connecting plate (311), each connecting plate (311) is fixedly connected with the collection box (1) through the corresponding connecting bolt (312) in a threaded manner, in the installation process, each connecting bolt (312) penetrates through the corresponding connecting plate (311) and is screwed into the corresponding collection box (1), the connecting bolts (312) are screwed out in the disassembly process, so that the upper cover (3) and the collection box (1) are installed and disassembled, a plurality of uniformly distributed infusion tubes (32) are arranged at the top of the upper cover (3), the positions of the infusion tubes (32) correspond to the first filter plates (22) of each group, and a plurality of uniformly distributed small electromagnetic valves, small-size solenoid valve (33) and outside PLC controller signal connection, small-size solenoid valve (33) output end fixedly connected with supply tube (331), and supply tube (331) keep away from the one end of small-size solenoid valve (33) and stretch into inside incubator (2), transfer line (32) are connected with small-size solenoid valve (33) input, transfer line (32) one end is connected with infusion hose (34), the one end that transfer line (32) were kept away from in infusion hose (34) is connected with outside water pump, one side fixedly connected with a plurality of evenly distributed's hatching lamp (35) of transfer line (32) are kept away from in upper cover (3), hatching lamp (35) and outside PLC controller signal connection.
The working principle of the invention is as follows: when in use, the device is firstly placed in a sterile environment, the active peptide collecting box (13) and the waste water collecting box (14) are respectively inserted into the placing groove (11), the active peptide collecting box (13) is inserted into the placing groove (11) above, the waste water collecting box (14) is inserted into the placing groove (11) below, the incubator (2) is connected with the collecting box (1), an operator peels the fertilized eggs and the eggshells, the peeled eggs are placed into the incubator (2) and are separated into one culture space by the first filter plate (22) and the separating plate (23), the upper cover (3) can be covered after the operation is finished, because the size of water molecules is zero four nanometers smaller than that of the active peptide, the size of protein is one to one hundred nanometers larger than that of the active peptide, and the sizes of the filter pores of the first ultrafiltration membrane (221) and the second ultrafiltration membrane (261) are smaller than one nanometer, therefore, active peptides and water molecules in the egg white can flow out through a first ultrafiltration membrane (221) on a first filter plate (22) and a second ultrafiltration membrane (261) on a second filter plate (26), the number of supply pipes (331) on an upper cover (3) corresponds to the number of each divided culture space, the supply pipes (331) are positioned above each divided culture space, hatching lamps (35) also correspond to each culture space, the hatching lamps (35) are positioned above each divided culture space, the temperature sensor (24) and the humidity sensor (25) on a partition plate (23) can sense the temperature and the humidity in each culture space, when the temperature is higher or lower, the temperature sensor (24) sends a signal to an external PLC controller, so that the heat emitted by the hatching lamps (35) can be adjusted, because water molecules also pass through the first ultrafiltration membrane (221) and the second ultrafiltration membrane (261), when the moisture of eggs in the culture space is reduced, the moisture sensor (25) can sense the moisture, a signal is sent to an external PLC (programmable logic controller) immediately, a small electromagnetic valve (33) corresponding to the culture space with water shortage is opened, an external water pump is started, so that nutrient solution is conveyed into the culture space with water shortage along a liquid conveying pipe (32) and a supply pipe (331), the eggs in the culture space can grow healthily, active peptides and water molecules which pass through the first ultrafiltration membrane (221) and the second ultrafiltration membrane (261) are collected by the active peptide collecting box (13), the size of a filter hole of a third ultrafiltration membrane (133) on the active peptide collecting box (13) is four-five nanometers at zero, only the water molecules can be allowed to pass through, and the active peptides and water molecules in the active peptide collecting box (13) can fall into the wastewater collecting box (14), therefore, water molecules and active peptides are separated, when the embryonic eggs develop for ten days to thirteen days in the culture space, the chicken inside the embryonic eggs are gradually shaped, the egg white is consumed, and at the moment, the embryonic eggs in the culture space can be taken out to be subjected to the next step of extracting the active peptides in the egg yolk.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.
Claims (9)
1. The extraction method of the bioactive peptide in the embryo eggs comprises a collection box (1), a culture box (2) and an upper cover (3), and is characterized in that the collection box (1) is provided with two standing grooves (11) which are distributed up and down, sliding grooves (111) are formed in two sides of the top of each standing groove (11), the top of the collection box (1) is provided with a hollow groove (12), a bioactive peptide collection box (13) is arranged in the standing groove (11) which is positioned above the collection box, and a wastewater collection box (14) is arranged in the standing groove (11) which is positioned below the collection box;
the incubator (2) is connected with the collection box (1) in the empty groove (12) in a clamping manner, the incubator (2) is detachably connected with the collection box (1), a plurality of first filter plates (22) which are uniformly distributed are fixedly connected inside the incubator (2), every two first filter plates (22) form a group, a first ultrafiltration membrane (221) is fixedly connected to one side, opposite to the two first filter plates (22) which form the group, of each group, and a plurality of partition plates (23) which are uniformly distributed are fixedly connected between the first filter plates (22) of each group;
upper cover (3) are located incubator (2) top, just upper cover (3) with the connection can be dismantled in collecting box (1), upper cover (3) top is equipped with a plurality of evenly distributed's transfer line (32), just the position and the every group of transfer line (32) first filter plate (22) are corresponding, transfer line (32) bottom fixed connection is equipped with a plurality of evenly distributed's small-size solenoid valve (33), small-size solenoid valve (33) and outside PLC controller signal connection, transfer line (32) with small-size solenoid valve (33) input is connected, transfer line (32) one end is connected with infusion hose (34), infusion hose (34) are kept away from the one end and the outside water pump of transfer line (32) are connected.
2. The method for extracting bioactive peptides from embryonated eggs, according to claim 1, wherein first sliding plates (131) are fixedly connected to two sides of the top of the bioactive peptide collecting box (13), the first sliding plates (131) are slidably connected with the collecting box (1) in the chute (111), third filter plates (132) are fixedly connected to the bottom of the bioactive peptide collecting box (13), third ultrafiltration membranes (133) are fixedly connected to the top of the third filter plates (132), second sliding plates (141) are fixedly connected to two sides of the top of the wastewater collecting box (14), and the second sliding plates (141) are slidably connected with the collecting box (1) in the chute (111).
3. The method for extracting bioactive peptides from embryo eggs, according to claim 1, characterized in that an installation plate (21) is fixedly connected to the outer wall of the incubator (2), an installation bolt (211) is arranged on the installation plate (21), and the installation plate (21) is fixed with the collection box (1) through the installation bolt (211).
4. The method for extracting bioactive peptides from embryonated eggs, according to claim 1, wherein a temperature sensor (24) is fixedly connected to the separation plate (23), the temperature sensor (24) is in signal connection with an external PLC (programmable logic controller), a humidity sensor (25) is fixedly connected to the separation plate (23), the humidity sensor (25) is in signal connection with the external PLC, a second filter plate (26) is fixedly connected to the bottom of the incubator (2), and a second ultrafiltration membrane (261) is fixedly connected to the top of the second filter plate (26).
5. The method for extracting bioactive peptides from embryo eggs according to claim 1, wherein longitudinal nameplates (27) are fixedly connected to the outer wall of the incubator (2), transverse nameplates (28) are fixedly connected to the outer wall of the incubator (2), a plurality of collecting grooves (29) are uniformly distributed in the incubator (2), and the collecting grooves (29) are positioned on two sides of each group of the first filter plates (22).
6. The method for extracting bioactive peptides from embryonated eggs, according to claim 1, wherein the four corners of the upper cover (3) are fixedly connected with connecting columns (31), one end of the connecting column (31) far away from the upper cover (3) is fixedly connected with a connecting plate (311), the connecting plate (311) is provided with a connecting bolt (312), and the connecting plate (311) is fixed with the collection box (1) through the connecting bolt (312) in a threaded manner.
7. The method for extracting bioactive peptide from embryo eggs, according to claim 1, characterized in that the output end of the small electromagnetic valve (33) is fixedly connected with a supply pipe (331), and one end of the supply pipe (331) far away from the small electromagnetic valve (33) extends into the incubator (2).
8. The method for extracting the bioactive peptide from the embryo eggs, as claimed in claim 1, wherein one side of the upper cover (3) far away from the infusion tube (32) is fixedly connected with a plurality of evenly distributed hatching lamps (35), and the hatching lamps (35) are in signal connection with an external PLC controller.
9. The method of any one of claims 1-8, wherein the method comprises the steps of: the using steps are as follows:
a: when the device is used, the device is firstly placed in a sterile environment, then the active peptide collecting box (13) and the waste water collecting box (14) are respectively inserted into the placing groove (11), the active peptide collecting box (13) is inserted into the placing groove (11) above, the waste water collecting box (14) is inserted into the placing groove (11) below, the incubator (2) is connected with the collecting box (1), an operator peels fertilized eggs and egg shells, the peeled eggs are placed into the incubator (2) and are separated into culture spaces by the first filter plate (22) and the partition plate (23), and the upper cover (3) can be covered after the operation is finished;
b: after the step A is finished, because the size of water molecules is zero four nanometers smaller than that of active peptides, the size of proteins is one to one hundred nanometers larger than that of the active peptides, and the sizes of filter pores of the first ultrafiltration membrane (221) and the second ultrafiltration membrane (261) are smaller than one nanometer, the active peptides and water molecules in the egg white can flow out through the first ultrafiltration membrane (221) on the first filter plate (22) and the second ultrafiltration membrane (261) on the second filter plate (26), the number of the supply pipes (331) on the upper cover (3) corresponds to the number of each divided culture space, the supply pipes (331) are positioned above each divided culture space, the hatching lamps (35) also correspond to each culture space, the hatching lamps (35) are positioned above each divided culture space, and the hatching lamps pass through the temperature sensors (24) and the humidity sensors (25) on the partition plates (23), the temperature and the humidity in each culture space can be sensed, when the temperature is high or low, the temperature sensor (24) sends a signal to an external PLC (programmable logic controller), so that the heat emitted by the hatching lamp (35) is adjusted, water molecules can also pass through the first ultrafiltration membrane (221) and the second ultrafiltration membrane (261), so that the humidity sensor (25) can sense when the moisture of eggs in the culture spaces is reduced, a signal is sent to the external PLC immediately, a small electromagnetic valve (33) corresponding to the water-deficient culture space is opened, an external water pump is started, so that nutrient solution is conveyed into the water-deficient culture space along the liquid conveying pipe (32) and the supply pipe (331), and the eggs in the culture space can grow healthily;
c: after the step B is completed, active peptides and water molecules which pass through the first ultrafiltration membrane (221) and the second ultrafiltration membrane (261) are collected by the active peptide collecting box (13), the size of a filtering hole of the third ultrafiltration membrane (133) on the active peptide collecting box (13) is zero four-five nanometers, only water molecules can be allowed to pass, the water molecules in the active peptide collecting box (13) fall into the waste water collecting box (14), so that the water molecules and the active peptides are separated, when the embryonated eggs develop in the culture space for ten days to thirteen days, the chickens in the embryonated eggs are gradually molded, egg white is consumed, and the embryonated eggs in the culture space can be taken out to be subjected to next extraction of the active peptides in the egg yolk.
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| CN202011468579.6A CN112321672A (en) | 2020-12-14 | 2020-12-14 | Method for extracting bioactive peptide from embryo eggs |
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| CN202011468579.6A CN112321672A (en) | 2020-12-14 | 2020-12-14 | Method for extracting bioactive peptide from embryo eggs |
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| CN (1) | CN112321672A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109125354A (en) * | 2018-10-31 | 2019-01-04 | 郭建德 | It is a kind of for eliminating or inhibiting the composition of carcinoma and soft tissue hyperplasia body |
| CN113122443A (en) * | 2021-04-23 | 2021-07-16 | 徐州生物工程职业技术学院 | Energy-saving fermentation device for producing polypeptide drugs |
-
2020
- 2020-12-14 CN CN202011468579.6A patent/CN112321672A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109125354A (en) * | 2018-10-31 | 2019-01-04 | 郭建德 | It is a kind of for eliminating or inhibiting the composition of carcinoma and soft tissue hyperplasia body |
| CN113122443A (en) * | 2021-04-23 | 2021-07-16 | 徐州生物工程职业技术学院 | Energy-saving fermentation device for producing polypeptide drugs |
| CN113122443B (en) * | 2021-04-23 | 2021-09-10 | 徐州生物工程职业技术学院 | Energy-saving fermentation device for producing polypeptide drugs |
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Application publication date: 20210205 |
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