CN204509350U - The device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide - Google Patents
The device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide Download PDFInfo
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- CN204509350U CN204509350U CN201520143625.3U CN201520143625U CN204509350U CN 204509350 U CN204509350 U CN 204509350U CN 201520143625 U CN201520143625 U CN 201520143625U CN 204509350 U CN204509350 U CN 204509350U
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Abstract
The utility model discloses the device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide, belong to food manufacturing apparatus technical field.It is characterized in that comprising enzymolysis tank body and ion-exchange ultra-filtration membrane, described enzymolysis tank body is provided with feed liquid port and enzyme entrance, stirring rake is provided with in enzymolysis tank body, described ion-exchange ultra-filtration membrane is arranged on described enzymolysis tank body lower part, be provided with permeate in the bottom of the enzymolysis tank body of the below of ion-exchange ultra-filtration membrane and collect outlet, the enzymolysis tank body of the upside of ion-exchange ultra-filtration membrane is provided with scavenging solution upper inlet and scavenging solution upper outlet, exports under the enzymolysis tank body of the downside of ion-exchange ultra-filtration membrane is provided with scavenging solution lower inlet and scavenging solution.Feature of the present utility model completes the separation of polypeptide and the purifying of high anti-oxidation active polypeptide wheatgerm extracting solution enzymolysis while, simplifies abstraction and purification process, required time is short, efficiency is high, convenient and swift, simple and practical.
Description
Technical field
The utility model relates to food manufacturing apparatus technical field.
Background technology
Wheat is the Source of life that the Nature gives human health existence, five cereals expensive.Wheatgerm is core and the life of wheat, plant is called embryo, be equivalent to the placenta of animal, although it only accounts for 2% of wheat weight, but nutrition accounts for 97% of whole wheat, wherein contain more than 50 kind of needed by human body abundant nutrition and some are not also by the micro-physiologically active ingredient of current scientific discovery, there is high nutritive value and pharmaceutical use, be described as " the natural nutrition treasure-houses of the mankind " by nutritionist, wheatgerm, to the mystery effect of HUMAN HEALTH, more and more obtains common people and pays close attention to and favor.
Albumen in wheatgerm generates after enzymic hydrolysis has bioactive polypeptide, and these high anti-oxidation active polypeptide are extracted, can be used for healthcare products, nourishing reinforced food etc. by the polypeptide wherein containing high anti-oxidation activity.What current research was more is from wheatgerm, extract high anti-oxidation active polypeptide, plays redoxomorphism, scavenging free radicals to human body cell, has the important physiological function such as anti-ageing, anticancer and removing toxic substances.But prior art is that carry out separation with ion exchange chromatography to enzymolysis solution and obtain high anti-oxidation active polypeptide, its complex manufacturing, process are loaded down with trivial details, occupation area of equipment is large by after wheatgerm extracting solution enzymolysis.
Utility model content
The technical problems to be solved in the utility model is for above-mentioned the deficiencies in the prior art, the device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide is provided, described device completes the separation of polypeptide and the purifying of high anti-oxidation active polypeptide while wheatgerm extracting solution enzymolysis, simplify purge process, required time is short, efficiency is high, convenient and swift, simple and practical.
For solving the problems of the technologies described above, technical solution adopted in the utility model is: the device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide, it is characterized in that comprising enzymolysis tank body and ion-exchange ultra-filtration membrane, described enzymolysis tank body is provided with feed liquid port and enzyme entrance, stirring rake is provided with in enzymolysis tank body, described ion-exchange ultra-filtration membrane is arranged on described enzymolysis tank body lower part, be provided with permeate in the bottom of the enzymolysis tank body of the below of ion-exchange ultra-filtration membrane and collect outlet, the enzymolysis tank body of the upside of ion-exchange ultra-filtration membrane is provided with scavenging solution upper inlet and scavenging solution upper outlet, export under the enzymolysis tank body of the downside of ion-exchange ultra-filtration membrane is provided with scavenging solution lower inlet and scavenging solution.
As preferably, the rotating speed of described stirring rake is 150-300rpm.
As preferably, the molecular weight cut-off of described ion-exchange ultra-filtration membrane is 1000 ~ 3000Da.
The beneficial effect adopting technique scheme to produce is: while wheatgerm extracting solution carries out enzymolysis in tank body, the high anti-oxidation active polypeptide generated is attracted on ultra-filtration membrane because of ionic interaction, collection capable of washing, other low molecular weight polypeptide is collected through ultra-filtration membrane, the separation of polypeptide and the enriching and purifying of high anti-oxidation active polypeptide is completed like this in the process of enzymolysis, simplify abstraction and purification process, required time be short, efficiency is high, convenient and swift, simple and practical.In addition because this device operational condition is gentle, some biologically active substances are not almost damaged, does not therefore affect the physico-chemical property of product in the operating condition, improve quality product.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Wherein: 1, feed liquid port; 2, enzyme entrance; 3, stirring rake; 4, enzymolysis tank body; 5, ion-exchange ultra-filtration membrane; 6, scavenging solution upper inlet; 7, scavenging solution lower inlet; 8, permeate collects outlet; 10, export under scavenging solution; 11, scavenging solution upper outlet.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in further detail.
The device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide, it is characterized in that comprising enzymolysis tank body 4 and ion-exchange ultra-filtration membrane 5, described enzymolysis tank body 4 is provided with feed liquid port 1 and enzyme entrance 2, stirring rake 3 is provided with in enzymolysis tank body 4, the rotating speed of stirring rake 3 is 150rpm, described ion-exchange ultra-filtration membrane 5 is arranged on described enzymolysis tank body 4 bottom, the molecular weight cut-off of ion-exchange ultra-filtration membrane 5 is 1000Da, be provided with permeate in the bottom of the enzymolysis tank body 4 of the below of ion-exchange ultra-filtration membrane 5 and collect outlet 8, the enzymolysis tank body 4 of the upside of ion-exchange ultra-filtration membrane 5 is provided with scavenging solution upper inlet 6 and scavenging solution upper outlet 11, 10 are exported under the enzymolysis tank body 4 of the downside of ion-exchange ultra-filtration membrane 5 being provided with scavenging solution lower inlet 7 and scavenging solution.
Wheatgerm extracting solution enters enzymolysis tank body 4 from feed liquid port 1, proteolytic enzyme enters enzymolysis tank body 4 from enzyme entrance 2, the abundant enzymolysis of wheatgerm extracting solution is made by the stirring action of stirring rake, while wheatgerm extracting solution carries out enzymolysis in enzymolysis tank body 4, ion-exchange ultra-filtration membrane 5 adsorbs high anti-oxidation active polypeptide, other low molecular weight polypeptide is through ion-exchange ultra-filtration membrane 5, collect outlet 8 from permeate to discharge, complete the separation of polypeptide and the purifying of high anti-oxidation active polypeptide simultaneously, after enzymolysis completes, scavenging solution enters enzymolysis tank body 4 from scavenging solution upper inlet 6 and scavenging solution lower inlet 7, the upside of the scavenging solution cleaning ion-exchange ultra-filtration membrane 5 that scavenging solution upper inlet 6 enters, be attracted to and discharge from scavenging solution upper outlet 11 along with scavenging solution at the high anti-oxidation active polypeptide of ion-exchange ultra-filtration membrane 5, the downside of the scavenging solution cleaning ion-exchange ultra-filtration membrane 5 that scavenging solution upper inlet 7 enters, the polypeptide be attached on the downside of ion-exchange ultra-filtration membrane 5 is discharged along with scavenging solution exports 10 from scavenging solution, complete the separation of polypeptide and the purifying of high anti-oxidation active polypeptide, required time is short, efficiency is high, convenient and swift, simple and practical.
Claims (3)
1.
onethe device of grow wheat plumule enzymolysis and purifying high anti-oxidation active polypeptide, it is characterized in that comprising enzymolysis tank body (4) and ion-exchange ultra-filtration membrane (5), described enzymolysis tank body (4) is provided with feed liquid port (1) and enzyme entrance (2), stirring rake (3) is provided with in enzymolysis tank body (4), described ion-exchange ultra-filtration membrane (5) is arranged on described enzymolysis tank body (4) bottom, the bottom of the enzymolysis tank body (4) of ion-exchange ultra-filtration membrane (5) below is provided with permeate and collects outlet (8), the enzymolysis tank body (4) of the upside of ion-exchange ultra-filtration membrane (5) is provided with scavenging solution upper inlet (6) and scavenging solution upper outlet (11), (10) are exported under the enzymolysis tank body (4) of the downside of ion-exchange ultra-filtration membrane (5) being provided with scavenging solution lower inlet (7) and scavenging solution.
2. the device of a kind of wheatgerm enzymolysis according to claim 1 and purifying high anti-oxidation active polypeptide, is characterized in that the rotating speed of described stirring rake (3) is 150-300rpm.
3. the device of a kind of wheatgerm enzymolysis according to claim 1 and purifying high anti-oxidation active polypeptide, is characterized in that the molecular weight cut-off of described ion-exchange ultra-filtration membrane (5) is 1000 ~ 3000Da.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520143625.3U CN204509350U (en) | 2015-03-14 | 2015-03-14 | The device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520143625.3U CN204509350U (en) | 2015-03-14 | 2015-03-14 | The device of a kind of wheatgerm enzymolysis and purifying high anti-oxidation active polypeptide |
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| CN204509350U true CN204509350U (en) | 2015-07-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106497781A (en) * | 2016-10-24 | 2017-03-15 | 世茂（苏州）生物科技有限公司 | A kind of collagen protein extracting method of Fish collagen protein without fishy smell extraction equipment and based on which |
| CN108308367A (en) * | 2018-04-10 | 2018-07-24 | 刘勇德 | The enzymolysis ultrafiltration apparatus of sea cucumber colla corii asini polypeptide nutrient product and the production method of sea cucumber colla corii asini polypeptide nutrient product |
| CN111137952A (en) * | 2020-03-23 | 2020-05-12 | 中新瑞美(天津)环保科技有限公司 | Preparation method of separation membrane for groundwater pollution remediation |
-
2015
- 2015-03-14 CN CN201520143625.3U patent/CN204509350U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106497781A (en) * | 2016-10-24 | 2017-03-15 | 世茂（苏州）生物科技有限公司 | A kind of collagen protein extracting method of Fish collagen protein without fishy smell extraction equipment and based on which |
| CN106497781B (en) * | 2016-10-24 | 2018-09-07 | 世茂（苏州）生物科技有限公司 | A kind of fish collagen is without fishy smell extraction equipment and based on its collagen extracting method |
| CN108308367A (en) * | 2018-04-10 | 2018-07-24 | 刘勇德 | The enzymolysis ultrafiltration apparatus of sea cucumber colla corii asini polypeptide nutrient product and the production method of sea cucumber colla corii asini polypeptide nutrient product |
| CN111137952A (en) * | 2020-03-23 | 2020-05-12 | 中新瑞美(天津)环保科技有限公司 | Preparation method of separation membrane for groundwater pollution remediation |
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Address after: 056900 Beijing Industrial City, Daming County, Handan City, Hebei Province Patentee after: Hebei Peifeng Biotechnology Co., Ltd. Address before: 056900 Beijing Industrial City, Daming County, Handan City, Hebei Province Patentee before: HEBEI ZIFENG BIOTECHNOLOGY CO., LTD. |
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| CP01 | Change in the name or title of a patent holder |