CN107376423B - A kind of column position valve and its application method isolating and purifying instrument for continuous chromatography - Google Patents

A kind of column position valve and its application method isolating and purifying instrument for continuous chromatography Download PDF

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CN107376423B
CN107376423B CN201710815322.5A CN201710815322A CN107376423B CN 107376423 B CN107376423 B CN 107376423B CN 201710815322 A CN201710815322 A CN 201710815322A CN 107376423 B CN107376423 B CN 107376423B
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solenoid valve
chromatographic column
column
valve
tail portion
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CN107376423A (en
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周胜
徐慧
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LISURE SCIENCE (SUZHOU) CO Ltd
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LISURE SCIENCE (SUZHOU) CO Ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K51/00—Other details not peculiar to particular types of valves or cut-off apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N30/20—Injection using a sampling valve
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N30/20—Injection using a sampling valve
    • G01N2030/201—Injection using a sampling valve multiport valves, i.e. having more than two ports

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

A kind of column position valve and its application method isolating and purifying instrument for continuous chromatography, belong to chemical industry, bio-pharmaceuticals equipment technical field, including the valve plate pedestal being made of long side valve plate, short side valve plate and top valve plate, lower valve plates, and the sampling pump of valve plate chassis outer side, elution pump, 4 chromatographic columns are set, the top valve plate of valve plate pedestal is equipped with the identical two-position two-way solenoid valve of 26 structures, and 26 two-position two-way solenoid valves constitute front-seat solenoid valve block, intermediate solenoid valve block and heel row solenoid valve block；The connection flow path of column position valve is equipped with inside valve plate pedestal.Structure of the invention novelty is compact, Method And Principle is clear, using 4 column systems and valve board structure, flow pipe is set inside valve plate, and loading does not stop, and saves the batch processed time, filler utilization rate is high, it can effectively avoid sample to be pierced by, save packing volume, save the batch processed time and shorten the production time and play advantageous effect.

Description

A kind of column position valve and its application method isolating and purifying instrument for continuous chromatography
Technical field
The invention belongs to chemical industry, bio-pharmaceuticals equipment technical field, it is related to a kind of chromatographic isolation equipment and its application method, Particularly relate to a kind of column position valve and its application method that instrument is isolated and purified for continuous chromatography.
Background technique
China possesses the hugest chemical composition of Chinese materia medica and natural drug structural research troop, is mainly engaged in natural products Extraction separation and Structural Identification research, extensive and economy the purifying of protein is increasingly becoming biotechnology and pharmacy work Major issue in industry, almost 80% or more work is all in the separation for carrying out native compound, this research field seems As labor-intensive manual labor, native compound is isolated and purified as natural drug initiative or lead compound hair Existing bottleneck, a large amount of time and efforts, which is wasted in, to be isolated and purified；And modern automation isolation technics equipment rapidly and efficiently is still It is the key that so breakthrough bottleneck.Therefore combined application chromatography new technology designs the competitions of various fast separation device equipment also just Very fierce, many equipment and technical method occur has very much, but to the height for completely solving various composition in a kind of medicinal plant Effect quick separating prepares purifier apparatus, actually rare, and in face of the complexity multiplicity of traditional Chinese medicine ingredients structure, and effect is preferably just more It is few.
Typical purification process includes differential centrifugation and filtration step and at least three kinds of chromatographic separation technologies, such as affine color Spectrometry (AC), gel permeation chromatography (GPC), ion-exchange chromatography (IEC), hydrophobic interaction chromatography (HIC), reverse-phase chromatography Method (RPC) and normal phase chromatography (NPC).Simple batch-type chromatographic technology is widely used in industrial application, however Since the processing time is long and operation cost is high (such as quantity of solvent is big, resin and expensive hardware), this technology is very expensive, this skill Art is also to service condition (such as product titre, residence time and charging rate) sensitivity.In comparison, continuous chromatography isolates and purifies The performance of instrument seems more prominent, however existing continuous chromatography isolates and purifies instrument equipment and constitutes complicated, cost of manufacture height, right Complex compound mixed system disengaging time is long, packing volume is big, the utilization efficiency of filler is not high, and production efficiency cannot be effective It is promoted.
Summary of the invention
It is right the purpose of the present invention is isolating and purifying equipment for above-mentioned existing continuous chromatography to constitute complicated, cost of manufacture height Complex compound mixed system disengaging time is long, packing volume is big, the utilization efficiency of filler is not high, and production efficiency cannot be effective The deficiencies of promotion, proposes a kind of to isolate and purify the column position valve of instrument for continuous chromatography and its application method can using valve board structure Effectively sample is avoided to be pierced by, make it to filler utilization efficiency is improved, save packing volume, save the batch processed time and shortens life The production time plays advantageous effect.
The technical scheme is that a kind of column position valve that instrument is isolated and purified for continuous chromatography, including valve plate pedestal, with And the sampling pump of valve plate chassis outer side, elution pump, chromatographic column 1, chromatographic column 2, chromatographic column 3 and chromatographic column 4 are set；Its feature exists In: the top valve plate of the valve plate pedestal is equipped with the identical two-position two-way solenoid valve of 26 structures, 26 bi-bit bi-pass Solenoid valve constitutes front-seat solenoid valve block, intermediate solenoid valve block and heel row solenoid valve block；
The front row solenoid valve block is by solenoid valve V5-1, solenoid valve V5-2, solenoid valve V5-3, solenoid valve V5-4, solenoid valve V3-1, solenoid valve V3-2, solenoid valve V3-3 and solenoid valve V3-4 are constituted；
The intermediate solenoid valve block is by solenoid valve V1-1, solenoid valve V1-2, solenoid valve V1-3, solenoid valve V1-4, solenoid valve V1-5, solenoid valve V2-1, solenoid valve V2-2, solenoid valve V2-3, solenoid valve V2-4 and solenoid valve V2-5 are constituted；
The heel row solenoid valve block is by solenoid valve V6-1, solenoid valve V6-2, solenoid valve V6-3, solenoid valve V6-4, solenoid valve V4-1, solenoid valve V4-2, solenoid valve V4-3 and solenoid valve V4-4 are constituted；
The connection flow path of column position valve is equipped with inside the valve plate pedestal, left short side valve plate is equipped with 1 head bore of column, 1 tail of column Portion hole, 2 head bore of column, 2 tail hole of column, 3 head bore of column, 4 head bore of column, elution pump connecting hole and product collecting terminal；Right short side Valve plate is equipped with 4 tail hole of column, 3 tail end hole of sampling pump connecting hole, waste collection end and column；1 head bore of column and 1 tail portion of column Hole and 1 headtotail of chromatographic column, 2 head bore of column and 2 tail hole of column and 2 headtotail of chromatographic column, the column 3 Head bore and 3 tail hole of column and 3 headtotail of chromatographic column, 4 head bore of column and 4 tail hole of column and the chromatographic column 4 Headtotail, the elution connecting hole connect with elution pump, and the sampling pump connecting hole connects with the sampling pump；
The connection flow path of column position valve constitutes as follows:
(1) the sampling pump connecting hole and the solenoid valve V1-1, solenoid valve V1-2, solenoid valve V1-3, solenoid valve V1-4, Solenoid valve V1-5 formation is connected in parallel；Wherein, the solenoid valve V1-1 is connected with the waste collection end；The solenoid valve V1-2 The product collecting terminal is connected to after connecting with solenoid valve V5-1, solenoid valve V5-2；The solenoid valve V1-3 and solenoid valve V4-1, The product collecting terminal is connected to after solenoid valve V4-2 series connection；The solenoid valve V1-4 and solenoid valve V3-1, solenoid valve V3-2 go here and there The product collecting terminal is connected to after connection；The solenoid valve V1-5 is connected to institute after connecting with solenoid valve V6-1, solenoid valve V6-2 State product collecting terminal；
(2) elution pump connecting hole and the solenoid valve V2-1, solenoid valve V2-2, solenoid valve V2-3, solenoid valve V2-4, Solenoid valve V2-5 formation is connected in parallel；Wherein, the solenoid valve V2-1 is connected to after connecting with solenoid valve V4-3, solenoid valve V4-4 The waste collection end；The solenoid valve V2-2 is connected to the waste collection after connecting with solenoid valve V5-3, solenoid valve V5-4 End；The solenoid valve V2-3 is connected to the waste collection end after connecting with solenoid valve V6-3, solenoid valve V6-4；The solenoid valve V2-4 is connected to the waste collection end after connecting with solenoid valve V3-3, solenoid valve V3-4；The solenoid valve V2-5 is connected to institute State product collecting terminal；
(3) 1 head bore of column and the outlet end of solenoid valve V1-5, solenoid valve V2-1 are connected in parallel；2 head bore of column It is connected in parallel with the outlet end of solenoid valve V1-4, solenoid valve V2-2；3 head bore of column and solenoid valve V1-3, solenoid valve V2-3 Outlet end be connected in parallel；4 head bore of column and the outlet end of solenoid valve V1-2, solenoid valve V2-4 are connected in parallel；
(4) 1 tail hole of column is formed in parallel with solenoid valve V3-1, solenoid valve V3-2, solenoid valve V3-3, solenoid valve V3-4 Connect；2 tail hole of column is connected in parallel with solenoid valve V4-1, solenoid valve V4-2, solenoid valve V4-3, solenoid valve V4-4 formation； 3 tail hole of column is connected in parallel with solenoid valve V5-1, solenoid valve V5-2, solenoid valve V5-3, solenoid valve V5-4 formation；The column 4 tail holes are connected in parallel with solenoid valve V6-1, solenoid valve V6-2, solenoid valve V6-3, solenoid valve V6-4 formation.
26 two-position two-way solenoid valves are normally off, and DN0.8mm, inlet and outlet pressure 6bar, KV value is 0.015M3/H, direct current 24VDC, power 3w.
The valve plate pedestal uses PEEK material, and long side valve plate is equipped with fabrication hole, is equipped with ceramic material in fabrication hole Spherical plug.
A kind of application method for the column position valve isolating and purifying instrument for continuous chromatography, which is characterized in that operating procedure is as follows:
(1) when opening solenoid valve V1-5, solenoid valve V2-4, solenoid valve V3-1, solenoid valve V4-1, solenoid valve V5-4 and electromagnetism Valve V6-2 realizes 1,2,3 sample introduction of chromatographic column, the function that chromatographic column 4 elutes；
(1-1) sampling pump, solenoid valve V1-5,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-1, chromatographic column 2 Portion, 2 tail portion of chromatographic column, solenoid valve V4-1,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-4, waste collection end；
(1-2) elutes pump, solenoid valve V2-4,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-2, product collecting terminal；
(2) when opening solenoid valve V1-4, solenoid valve V2-1, solenoid valve V3-3, solenoid valve V4-1, solenoid valve V5-4 and electromagnetism Valve V6-2 realizes chromatographic column 2,3 sample introductions, the function that chromatographic column 1,4 elutes；
(2-1) sampling pump, solenoid valve V1-4,2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-1, chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve V5-4, waste collection end；
(2-2) elutes pump, solenoid valve V2-1,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-3, chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-2, product collecting terminal；
(3) when opening solenoid valve V1-4, solenoid valve V2-1, solenoid valve V3-2, solenoid valve V4-1, solenoid valve V5-1 and electromagnetism Valve V6-4 realizes 2,3,4 sample introduction of chromatographic column, the function that chromatographic column 1 elutes；
(3-1) sampling pump, solenoid valve V1-4,2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-1, chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve V5-1,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-4, waste collection end；
(3-2) elutes pump, solenoid valve V2-1,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-2, product collecting terminal；
(4) when opening solenoid valve V1-3, solenoid valve V2-2, solenoid valve V3-2, solenoid valve V4-3, solenoid valve V5-1 and electromagnetism Valve V6-4 realizes chromatographic column 3,4 sample introductions, the function that chromatographic column 1,2 elutes；
(4-1) sampling pump, solenoid valve V1-3,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-1, chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-4, waste collection end；
(4-2) elutes pump, solenoid valve V2-2,2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-3, chromatographic column 1 Portion, 1 tail portion of chromatographic column, solenoid valve V3-2, product collecting terminal；
(5) when opening solenoid valve V1-3, solenoid valve V2-2, solenoid valve V3-4, solenoid valve V4-2, solenoid valve V5-1 and electromagnetism Valve V6-1 realizes 1,3,4 sample introduction of chromatographic column, the function that chromatographic column 2 elutes；
(5-1) sampling pump, solenoid valve V1-3,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-1, chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-1,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-4, waste collection end；
(5-2) elutes pump, solenoid valve V2-2,2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-2, product collecting terminal；
(6) when opening solenoid valve V1-2, solenoid valve V2-3, solenoid valve V3-4, solenoid valve V4-2, solenoid valve V5-3 and electromagnetism Valve V6-1 realizes chromatographic column 1,4 sample introductions, the function that chromatographic column 2,3 elutes；
(6-1) sampling pump, solenoid valve V1-2,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-1, chromatographic column 1 Portion, 1 tail portion of chromatographic column, solenoid valve V3-4, waste collection end；
(6-2) elutes pump, solenoid valve V2-3,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-3, chromatographic column 2 Portion, 2 tail portion of chromatographic column, solenoid valve V4-2, product collecting terminal；
(7) when opening solenoid valve V1-2, solenoid valve V2-3, solenoid valve V3-1, solenoid valve V4-4, solenoid valve V5-2 and electromagnetism Valve V6-1 realizes 1,2,4 sample introduction of chromatographic column, the function that chromatographic column 3 elutes；
(7-1) sampling pump, solenoid valve V1-2,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-1, chromatographic column 1 Portion, 1 tail portion of chromatographic column, solenoid valve V3-1,2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-4, waste collection end；
(7-2) elutes pump, solenoid valve V2-3,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-2, product collecting terminal；
(8) when opening solenoid valve V1-5, solenoid valve V2-4, solenoid valve V3-1, solenoid valve V4-4, solenoid valve V5-2 and electromagnetism Valve V6-3 realizes chromatographic column 1,2 sample introductions, the function that chromatographic column 3,4 elutes；
(8-1) sampling pump, solenoid valve V1-5,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-1, chromatographic column 2 Portion, 2 tail portion of chromatographic column, solenoid valve V4-4, waste collection end；
(8-2) elutes pump, solenoid valve V2-4,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-3, chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve 5-2, product collecting terminal；
(9) solenoid valve V1-1 is only opened, realizes the function of sampling pump emptying；
(10) solenoid valve V2-5 is only opened, realizes the function of elution pump emptying；
(11) above-mentioned steps (1) is returned to, carries out repetitive operation.
Usefulness of the present invention: proposed by the present invention a kind of to isolate and purify the column position valve of instrument for continuous chromatography and its make With method, structure novel is compact, and Method And Principle is clear, and using 4 column systems and valve board structure, flow pipe is arranged inside valve plate, Loading does not stop, and saves the batch processed time, and filler utilization rate is high, can effectively avoid sample and is pierced by, and saves packing volume, saves Batch processed time and shortening production time play advantageous effect.
Detailed description of the invention
Fig. 1 is column position valve overall structure diagram of the present invention.
Fig. 2 is column position valve overlooking structure diagram of the present invention.
Fig. 3 is left short side valve plate board face structure schematic diagram in the valve of column position of the present invention.
Fig. 4 is right short side valve plate board face structure schematic diagram in the valve of column position of the present invention.
Fig. 5 is column position of the present invention valve schematic layout pattern in purifying instrument flow path.
Fig. 6 is valve internal flow path schematic diagram in column position of the present invention.
Fig. 7 is valve first state flow path schematic diagram in column position of the present invention.
Fig. 8 is column position of the present invention valve the second state flow path schematic diagram.
Fig. 9 is valve third state flow path schematic diagram in column position of the present invention.
Figure 10 is the 4th state flow path schematic diagram of column position of the present invention valve.
Figure 11 is the 5th state flow path schematic diagram of column position of the present invention valve.
Figure 12 is the 6th state flow path schematic diagram of column position of the present invention valve.
Figure 13 is the 7th state flow path schematic diagram of column position of the present invention valve.
Figure 14 is column position valve of the present invention state flow path schematic diagram eight-shaped.
Figure 15 is each electromagnetic valve work state diagram in the valve of column column of the present invention position.
In figure: the left short side valve plate of 1 valve plate pedestal, 1-1 long side valve plate, 1-2,1 tail hole of 1-2-1 column, 4 head of 1-2-2 column Hole, 2 head bore of 1-2-3 column, 1-2-4 product collecting terminal, 1 head bore of 1-2-5 column, 1-2-6 elution pump connecting hole, 2 tail of 1-2-7 column Portion hole, 3 head bore of 1-2-8 column, the top 1-3 valve plate, the right short side valve plate of 1-4,4 tail hole of 1-4-1 column, the connection of 1-4-2 sampling pump Hole, 1-4-3 waste collection end, 3 tail hole of 1-4-4 column, 2 front-seat solenoid valve blocks, 21 solenoid valve V5-1,22 solenoid valve V5-2,23 Solenoid valve V5-3,24 solenoid valve V5-4,25 solenoid valve V3-1,26 solenoid valve V3-2,27 solenoid valve V3-3,28 solenoid valve V3-4, 31 solenoid valve V1-1,32 solenoid valve V1-2,33 solenoid valve V1-3,34 solenoid valve V1-4,35 solenoid valve V1-5,36 solenoid valve V2- 1,37 solenoid valve V2-2,38 solenoid valve V2-3,39 solenoid valve V2-4,40 solenoid valve V2-5,41 solenoid valve V6-1,42 solenoid valves V6-2,43 solenoid valve V6-3,44 solenoid valve V6-4,45 solenoid valve V4-1,46 solenoid valve V4-2,47 solenoid valve V4-3,48 electromagnetism Valve V4-4, intermediate solenoid valve block 3, heel row solenoid valve block 4, link electrode 5.
Specific embodiment
The present invention will be further explained below with reference to the attached drawings:
As shown in Figs 1-4, a kind of column position valve isolating and purifying instrument for continuous chromatography, including valve plate pedestal 1, and setting In the sampling pump in 1 outside of valve plate pedestal, elution pump, chromatographic column 1, chromatographic column 2, chromatographic column 3 and chromatographic column 4；The top of valve plate pedestal 1 Portion valve plate 1-3 is equipped with the identical two-position two-way solenoid valve of 26 structures, and 26 two-position two-way solenoid valves constitute front-seat solenoid valve Group 2, intermediate solenoid valve block 3 and heel row solenoid valve block 4；Front-seat solenoid valve block 2 is by solenoid valve V5-1, solenoid valve V5-2, solenoid valve V5-3, solenoid valve V5-4, solenoid valve V3-1, solenoid valve V3-2, solenoid valve V3-3 and solenoid valve V3-4 are constituted；Intermediate solenoid valve block By solenoid valve V1-1, solenoid valve V1-2, solenoid valve V1-3, solenoid valve V1-4, solenoid valve V1-5, solenoid valve V2-1, solenoid valve V2- 2, solenoid valve V2-3, solenoid valve V2-4 and solenoid valve V2-5 are constituted；Heel row solenoid valve block by solenoid valve V6-1, solenoid valve V6-2, Solenoid valve V6-3, solenoid valve V6-4, solenoid valve V4-1, solenoid valve V4-2, solenoid valve V4-3 and solenoid valve V4-4 are constituted；26 two Position two-way electromagnetic valve is normally off, and DN0.8mm, inlet and outlet pressure 6bar, KV value is 0.015M3/H, direct current 24VDC, power 3w.Valve plate pedestal 1 uses PEEK material, and long side valve plate 1-1 is equipped with fabrication hole, and the spherical shape equipped with ceramic material is stifled in fabrication hole Head.
As shown in Figs 1-4, a kind of column position valve isolating and purifying instrument for continuous chromatography is equipped with column position inside valve plate pedestal 1 The connection flow path of valve, left short side valve plate 1-2 be equipped with 1 head bore 1-2-5 of column, 1 tail hole 1-2-1 of column, 2 head bore 1-2-3 of column, 2 tail hole 1-2-7 of column, 3 head bore 1-2-8 of column, 4 head bore 1-2-2 of column, elution pump connecting hole 1-2-6 and product collecting terminal 1- 2-4；Right short side valve plate 1-4 is equipped with 4 tail hole 1-4-1 of column, sampling pump connecting hole 1-4-2, waste collection end 1-4-3 and column 3 Tail hole 1-4-4；1 head bore 1-2-5 of column and 1 tail hole 1-2-1 of column and 1 headtotail of chromatographic column, 2 head bore 1-2-3 of column and 2 tail hole 1-2-7 of column and 2 headtotail of chromatographic column, 3 head bore 1-2-8 of column and 3 tail hole 1-4-4 of column and chromatographic column 3 phase end to end It connects, 4 head bore 1-2-2 of column and 4 tail hole 1-4-1 of column and 4 headtotail of chromatographic column, elution pump connecting hole 1-2-6 and elution are pumped Connect, sampling pump connecting hole 1-4-2 connects with sampling pump；
As shown in Figs 1-4, the connection flow path of a kind of column position valve isolating and purifying instrument for continuous chromatography, column position valve is constituted such as Under:
(1) sampling pump connecting hole 1-4-2 and solenoid valve V1-1, solenoid valve V1-2, solenoid valve V1-3, solenoid valve V1-4, electricity Magnet valve V1-5 formation is connected in parallel；Wherein, solenoid valve V1-1 is connected with waste collection end 1-4-3；Solenoid valve V1-2 and solenoid valve Product collecting terminal 1-2-4 is connected to after V5-1, solenoid valve V5-2 series connection；Solenoid valve V1-3 and solenoid valve V4-1, solenoid valve V4-2 Product collecting terminal 1-2-4 is connected to after series connection；Solenoid valve V1-4 is connected to product after connecting with solenoid valve V3-1, solenoid valve V3-2 Collecting terminal 1-2-4；Solenoid valve V1-5 is connected to product collecting terminal 1-2-4 after connecting with solenoid valve V6-1, solenoid valve V6-2；
(2) elution pump connecting hole 1-2-6 and solenoid valve V2-1, solenoid valve V2-2, solenoid valve V2-3, solenoid valve V2-4, electricity Magnet valve V2-5 formation is connected in parallel；Wherein, solenoid valve V2-1 is connected to waste liquid receipts after connecting with solenoid valve V4-3, solenoid valve V4-4 Collect end 1-4-3；Solenoid valve V2-2 is connected to waste collection end 1-4-3 after connecting with solenoid valve V5-3, solenoid valve V5-4；Solenoid valve V2-3 is connected to waste collection end 1-4-3 after connecting with solenoid valve V6-3, solenoid valve V6-4；Solenoid valve V2-4 and solenoid valve V3- 3, waste collection end 1-4-3 is connected to after solenoid valve V3-4 series connection；Solenoid valve V2-5 is connected to product collecting terminal 1-2-4；
(3) 1 head bore 1-2-5 of column and the outlet end of solenoid valve V1-5, solenoid valve V2-1 are connected in parallel；2 head bore 1- of column The outlet end of 2-3 and solenoid valve V1-4, solenoid valve V2-2 are connected in parallel；3 head bore 1-2-8 of column and solenoid valve V1-3, solenoid valve The outlet end of V2-3 is connected in parallel；4 head bore 1-2-2 of column and the outlet end of solenoid valve V1-2, solenoid valve V2-4 are connected in parallel；
(4) 1 tail hole 1-2-1 of column and solenoid valve V3-1, solenoid valve V3-2, solenoid valve V3-3, solenoid valve V3-4 are formed simultaneously Connection connects；2 tail hole 1-2-7 of column forms phase in parallel with solenoid valve V4-1, solenoid valve V4-2, solenoid valve V4-3, solenoid valve V4-4 It connects；Column 3 tail hole 1-4-4 and solenoid valve V5-1, solenoid valve V5-2, solenoid valve V5-3, solenoid valve V5-4 formation are connected in parallel；Column 4 tail hole 1-4-1 and solenoid valve V6-1, solenoid valve V6-2, solenoid valve V6-3, solenoid valve V6-4 formation are connected in parallel.
A kind of application method for the column position valve isolating and purifying instrument for continuous chromatography, operating procedure are as follows:
As shown in Fig. 5,7,15, solenoid valve V1-5, solenoid valve V2-4, solenoid valve V3-1, solenoid valve V4-1, solenoid valve are opened V5-4 and solenoid valve V6-2 realizes 1,2,3 sample introduction of chromatographic column, the function that chromatographic column 4 elutes；
Sampling pump, solenoid valve V1-5,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-1,2 head of chromatographic column, chromatography 2 tail portion of column, solenoid valve V4-1,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-4, waste collection end 1-4-3；Elution pump, Solenoid valve V2-4,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,8,15, solenoid valve V1-4, solenoid valve V2-1, solenoid valve V3-3, solenoid valve V4-1, electromagnetism are opened Valve V5-4 and solenoid valve V6-2 realizes chromatographic column 2,3 sample introductions, the function that chromatographic column 1,4 elutes；Sampling pump, solenoid valve V1-4, layer Analyse 2 head of column, 2 tail portion of chromatographic column, solenoid valve V4-1,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-4, waste collection Hold 1-4-3；Elute pump, solenoid valve V2-1,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-3,4 head of chromatographic column, chromatography 4 tail portion of column, solenoid valve V6-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,9,15, solenoid valve V1-4, solenoid valve V2-1, solenoid valve V3-2, solenoid valve V4-1, electromagnetism are opened Valve V5-1 and solenoid valve V6-4 realizes 2,3,4 sample introduction of chromatographic column, the function that chromatographic column 1 elutes；Sampling pump, solenoid valve V1-4, layer Analyse 2 head of column, 2 tail portion of chromatographic column, solenoid valve V4-1,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-1, chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-4, waste collection end 1-4-3；Elute pump, solenoid valve V2-1,1 head of chromatographic column, chromatography 1 tail portion of column, solenoid valve V3-2, product collecting terminal；
As shown in Fig. 5,6,10,15, solenoid valve V1-3, solenoid valve V2-2, solenoid valve V3-2, solenoid valve V4-3, electricity are opened Magnet valve V5-1 and solenoid valve V6-4 realizes chromatographic column 3,4 sample introductions, the function that chromatographic column 1,2 elutes；Sampling pump, solenoid valve V1-3, 3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-1,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-4, waste liquid are received Collect end 1-4-3；Elute pump, solenoid valve V2-2,2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-3,1 head of chromatographic column, layer Analyse 1 tail portion of column, solenoid valve V3-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,11,15, solenoid valve V1-3, solenoid valve V2-2, solenoid valve V3-4, solenoid valve V4-2, electricity are opened Magnet valve V5-1 and solenoid valve V6-1 realizes 1,3,4 sample introduction of chromatographic column, the function that chromatographic column 2 elutes；Sampling pump, solenoid valve V1-3, 3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-1,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-1, chromatographic column 1 Head, 1 tail portion of chromatographic column, solenoid valve V3-4, waste collection end 1-4-3；Elute pump, solenoid valve V2-2,2 head of chromatographic column, layer Analyse 2 tail portion of column, solenoid valve V4-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,12,15, solenoid valve V1-2, solenoid valve V2-3, solenoid valve V3-4, solenoid valve V4-2, electricity are opened Magnet valve V5-3 and solenoid valve V6-1 realizes chromatographic column 1,4 sample introductions, the function that chromatographic column 2,3 elutes；Sampling pump, solenoid valve V1-2, 4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-1,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-4, waste liquid are received Collect end 1-4-3；Elute pump, solenoid valve V2-3,3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-3,2 head of chromatographic column, layer Analyse 2 tail portion of column, solenoid valve V4-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,13,15, solenoid valve V1-2, solenoid valve V2-3, solenoid valve V3-1, solenoid valve V4-4, electricity are opened Magnet valve V5-2 and solenoid valve V6-1 realizes 1,2,4 sample introduction of chromatographic column, the function that chromatographic column 3 elutes；Sampling pump, solenoid valve V1-2, 4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-1,1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-1, chromatographic column 2 Head, 2 tail portion of chromatographic column, solenoid valve V4-4, waste collection end 1-4-3；Elute pump, solenoid valve V2-3,3 head of chromatographic column, layer Analyse 3 tail portion of column, solenoid valve V5-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,14,15, solenoid valve V1-5, solenoid valve V2-4, solenoid valve V3-1, solenoid valve V4-4, electricity are opened Magnet valve V5-2 and solenoid valve V6-3 realizes chromatographic column 1,2 sample introductions, the function that chromatographic column 3,4 elutes；Sampling pump, solenoid valve V1-5, 1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-1,2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-4, waste liquid are received Collect end 1-4-3；Elute pump, solenoid valve V2-4,4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-3,3 head of chromatographic column, layer Analyse 3 tail portion of column, solenoid valve 5-2, product collecting terminal 1-2-4；
If solenoid valve V1-1 is opened in Fig. 5,6,15, the function of sampling pump emptying is realized；Solenoid valve V2-5 is opened, realizes elution Pump the function of emptying.

Claims (4)

1. a kind of column position valve for isolating and purifying instrument for continuous chromatography, including valve plate pedestal (1), setting valve plate pedestal (1) outside The sampling pump of side elutes pump, chromatographic column 1, chromatographic column 2, chromatographic column 3 and chromatographic column 4；It is characterized by: the valve plate pedestal (1) Top valve plate (1-3) be equipped with the identical two-position two-way solenoid valve of 26 structures, 26 two-position two-way solenoid valves are constituted Front-seat solenoid valve block (2), intermediate solenoid valve block (3) and heel row solenoid valve block (4)；
The front row solenoid valve block (2) is by solenoid valve V5-1 (21), solenoid valve V5-2 (22), solenoid valve V5-3 (23), solenoid valve V5-4 (24), solenoid valve V3-1 (25), solenoid valve V3-2 (26), solenoid valve V3-3 (27) and solenoid valve V3-4 (28) are constituted；
The intermediate solenoid valve block is by solenoid valve V1-1 (31), solenoid valve V1-2 (32), solenoid valve V1-3 (33), solenoid valve V1-4 (34), solenoid valve V1-5 (35), solenoid valve V2-1 (36), solenoid valve V2-2 (37), solenoid valve V2-3 (38), solenoid valve V2-4 (39) it is constituted with solenoid valve V2-5 (40)；
The heel row solenoid valve block is by solenoid valve V6-1 (41), solenoid valve V6-2 (42), solenoid valve V6-3 (43), solenoid valve V6-4 (44), solenoid valve V4-1 (45), solenoid valve V4-2 (46), solenoid valve V4-3 (47) and solenoid valve V4-4 (48) are constituted；
The connection flow path of column position valve is equipped with inside the valve plate pedestal (1), left short side valve plate (1-2) is equipped with 1 head bore (1- of column 2-5), 1 tail hole of column (1-2-1), 2 head bore of column (1-2-3), 2 tail hole of column (1-2-7), 3 head bore of column (1-2-8), column 4 Head bore (1-2-2), elution pump connecting hole (1-2-6) and product collecting terminal (1-2-4)；Right short side valve plate (1-4) is equipped with column 4 Tail hole (1-4-1), sampling pump connecting hole (1-4-2), waste collection end (1-4-3) and 3 tail hole of column (1-4-4)；The column 1 Head bore (1-2-5) and 1 tail hole of column (1-2-1) and 1 headtotail of chromatographic column, 2 head bore of column (1-2-3) and column 2 tail holes (1-2-7) and 2 headtotail of chromatographic column, 3 head bore of column (1-2-8) and 3 tail hole of column (1-4-4) and 3 headtotail of chromatographic column, 4 head bore of column (1-2-2) and 4 tail hole of column (1-4-1) and the chromatographic column 4 phase end to end It connects, elution pump connecting hole (1-2-6) connects with elution pump, the sampling pump connecting hole (1-4-2) and the sample introduction Pump connects；
The connection flow path of column position valve constitutes as follows:
(1) the sampling pump connecting hole (1-4-2) and the solenoid valve V1-1 (31), solenoid valve V1-2 (32), solenoid valve V1-3 (33), solenoid valve V1-4 (34), solenoid valve V1-5 (35) formation is connected in parallel；Wherein, the solenoid valve V1-1 (31) with it is described Waste collection end (1-4-3) is connected；After the solenoid valve V1-2 (32) is connected with solenoid valve V5-1 (21), solenoid valve V5-2 (22) It is connected to the product collecting terminal (1-2-4)；The solenoid valve V1-3 (33) and solenoid valve V4-1 (45), solenoid valve V4-2 (46) The product collecting terminal (1-2-4) is connected to after series connection；The solenoid valve V1-4 (34) and solenoid valve V3-1 (25), solenoid valve The product collecting terminal (1-2-4) is connected to after V3-2 (26) series connection；The solenoid valve V1-5 (35) and solenoid valve V6-1 (41), The product collecting terminal (1-2-4) is connected to after solenoid valve V6-2 (42) series connection；
(2) elution pump connecting hole (1-2-6) and the solenoid valve V2-1 (36), solenoid valve V2-2 (37), solenoid valve V2-3 (38), solenoid valve V2-4 (39), solenoid valve V2-5 (40) formation is connected in parallel；Wherein, the solenoid valve V2-1 (36) and electromagnetism The waste collection end (1-4-3) is connected to after valve V4-3 (47), solenoid valve V4-4 (48) series connection；The solenoid valve V2-2 (37) The waste collection end (1-4-3) is connected to after connecting with solenoid valve V5-3 (23), solenoid valve V5-4 (24)；The solenoid valve V2-3 (38) is connected to the waste collection end (1-4-3) after connecting with solenoid valve V6-3 (43), solenoid valve V6-4 (44)；It is described Solenoid valve V2-4 (39) is connected to the waste collection end (1-4- after connecting with solenoid valve V3-3 (27), solenoid valve V3-4 (28) 3)；The solenoid valve V2-5 (40) is connected to the product collecting terminal (1-2-4)；
(3) 1 head bore of column (1-2-5) and the outlet end of solenoid valve V1-5 (35), solenoid valve V2-1 (36) are connected in parallel；Institute It states 2 head bore of column (1-2-3) and the outlet end of solenoid valve V1-4 (34), solenoid valve V2-2 (37) is connected in parallel；3 head of column Hole (1-2-8) and the outlet end of solenoid valve V1-3 (33), solenoid valve V2-3 (38) are connected in parallel；4 head bore of column (1-2-2) It is connected in parallel with the outlet end of solenoid valve V1-2 (32), solenoid valve V2-4 (39)；
(4) 1 tail hole of column (1-2-1) and solenoid valve V3-1 (25), solenoid valve V3-2 (26), solenoid valve V3-3 (27), electricity Magnet valve V3-4 (28) formation is connected in parallel；2 tail hole of column (1-2-7) and solenoid valve V4-1 (45), solenoid valve V4-2 (46), Solenoid valve V4-3 (47), solenoid valve V4-4 (48) formation are connected in parallel；3 tail hole of column (1-4-4) and solenoid valve V5-1 (21), solenoid valve V5-2 (22), solenoid valve V5-3 (23), solenoid valve V5-4 (24) formation are connected in parallel；4 tail hole of column (1-4-1) is in parallel with solenoid valve V6-1 (41), solenoid valve V6-2 (42), solenoid valve V6-3 (43), solenoid valve V6-4 (44) formation Connect.
2. a kind of column position valve for isolating and purifying instrument for continuous chromatography according to claim 1, it is characterised in that: described 26 Two-position two-way solenoid valve is normally off, DN0.8mm, and inlet and outlet pressure 6bar, KV value is 0.015M3/H, direct current 24VDC, Power 3w.
3. a kind of column position valve for isolating and purifying instrument for continuous chromatography according to claim 1, it is characterised in that: the valve Board bottom seat (1) uses PEEK material, and long side valve plate (1-1) is equipped with fabrication hole, and the spherical shape equipped with ceramic material is stifled in fabrication hole Head.
4. a kind of application method for the column position valve for isolating and purifying instrument for continuous chromatography as described in claim 1, feature exist In operating procedure is as follows:
(1) solenoid valve V1-5 (35), solenoid valve V2-4 (39), solenoid valve V3-1 (25), solenoid valve V4-1 (45), solenoid valve are opened V5-4 (24) and solenoid valve V6-2 (42) realizes 1,2,3 sample introduction of chromatographic column, the function that chromatographic column 4 elutes；
(1-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively by solenoid valve V1-5 (35), 1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-1 (25), 2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-1 (45), chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve V5-4 (24), waste collection end (1-4-3)；
(1-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-4 (39), chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-2 (42), product collecting terminal (1-2-4)；
(2) solenoid valve V1-4 (34), solenoid valve V2-1 (36), solenoid valve V3-3 (27), solenoid valve V4-1 (45), electromagnetism are opened Valve V5-4 (24) and solenoid valve V6-2 (42) realizes chromatographic column 2,3 sample introductions, the function that chromatographic column 1,4 elutes；
(2-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively pass through solenoid valve V1-4 (34), chromatographic column 2 Portion, 2 tail portion of chromatographic column, solenoid valve V4-1 (45), 3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-4 (24), waste collection It holds (1-4-3)；
(2-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-1 (36), chromatographic column 1 Portion, 1 tail portion of chromatographic column, solenoid valve V3-3 (27), 4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-2 (42), product are collected It holds (1-2-4)；
(3) solenoid valve V1-4 (34), solenoid valve V2-1 (36), solenoid valve V3-2 (26), solenoid valve V4-1 (45), solenoid valve are opened V5-1 (21) and solenoid valve V6-4 (44) realizes 2,3,4 sample introduction of chromatographic column, the function that chromatographic column 1 elutes；
(3-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively pass through solenoid valve V1-4 (34), chromatographic column 2 Portion, 2 tail portion of chromatographic column, solenoid valve V4-1 (45), 3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve V5-1 (21), chromatographic column 4 Head, 4 tail portion of chromatographic column, solenoid valve V6-4 (44), waste collection end (1-4-3)；
(3-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-1 (36), chromatographic column 1 Portion, 1 tail portion of chromatographic column, solenoid valve V3-2 (26), product collecting terminal (1-2-4)；
(4) solenoid valve V1-3 (33), solenoid valve V2-2 (37), solenoid valve V3-2 (26), solenoid valve V4-3 (47), solenoid valve are opened V5-1 (21) and solenoid valve V6-4 (44) realizes chromatographic column 3,4 sample introductions, the function that chromatographic column 1,2 elutes；
(4-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively pass through solenoid valve V1-3 (33), chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve V5-1 (21), 4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-4 (44), waste collection It holds (1-4-3)；
(4-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-2 (37), chromatographic column 2 Portion, 2 tail portion of chromatographic column, solenoid valve V4-3 (47), 1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-2 (26), product are collected It holds (1-2-4)；
(5) solenoid valve V1-3 (33), solenoid valve V2-2 (37), solenoid valve V3-4 (28), solenoid valve V4-2 (46), solenoid valve are opened V5-1 (21) and solenoid valve V6-1 (41) realizes 1,3,4 sample introduction of chromatographic column, the function that chromatographic column 2 elutes；
(5-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively pass through solenoid valve V1-3 (33), chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve V5-1 (21), 4 head of chromatographic column, 4 tail portion of chromatographic column, solenoid valve V6-1 (41), chromatographic column 1 Head, 1 tail portion of chromatographic column, solenoid valve V3-4 (28), waste collection end (1-4-3)；
(5-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-2 (37), chromatographic column 2 Portion, 2 tail portion of chromatographic column, solenoid valve V4-2 (46), product collecting terminal (1-2-4)；
(6) solenoid valve V1-2 (32), solenoid valve V2-3 (38), solenoid valve V3-4 (28), solenoid valve V4-2 (46), solenoid valve are opened V5-3 (23) and solenoid valve V6-1 (41) realizes chromatographic column 1,4 sample introductions, the function that chromatographic column 2,3 elutes；
(6-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively pass through solenoid valve V1-2 (32), chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-1 (41), 1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-4 (28), waste collection It holds (1-4-3)；
(6-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-3 (38), chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve V5-3 (23), 2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-2 (46), product are collected It holds (1-2-4)；
(7) solenoid valve V1-2 (32), solenoid valve V2-3 (38), solenoid valve V3-1 (25), solenoid valve V4-4 (48), solenoid valve are opened V5-2 (22) and solenoid valve V6-1 (41) realizes 1,2,4 sample introduction of chromatographic column, the function that chromatographic column 3 elutes；
(7-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively pass through solenoid valve V1-2 (32), chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-1 (41), 1 head of chromatographic column, 1 tail portion of chromatographic column, solenoid valve V3-1 (25), chromatographic column 2 Head, 2 tail portion of chromatographic column, solenoid valve V4-4 (48), waste collection end (1-4-3)；
(7-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-3 (38), chromatographic column 3 Portion, 3 tail portion of chromatographic column, solenoid valve V5-2 (22), product collecting terminal (1-2-4)；
(8) solenoid valve V1-5 (35), solenoid valve V2-4 (39), solenoid valve V3-1 (25), solenoid valve V4-4 (48), solenoid valve are opened V5-2 (22) and solenoid valve V6-3 (43) realizes chromatographic column 1,2 sample introductions, the function that chromatographic column 3,4 elutes；
(8-1) at this time sampling pump end fluid specific flow path sequence are as follows: successively pass through solenoid valve V1-5 (35), chromatographic column 1 Portion, 1 tail portion of chromatographic column, solenoid valve V3-1 (25), 2 head of chromatographic column, 2 tail portion of chromatographic column, solenoid valve V4-4 (48), waste collection It holds (1-4-3)；
(8-2) elution at this time pumps the specific flow path sequence of end fluid are as follows: successively passes through solenoid valve V2-4 (39), chromatographic column 4 Portion, 4 tail portion of chromatographic column, solenoid valve V6-3 (43), 3 head of chromatographic column, 3 tail portion of chromatographic column, solenoid valve 5-2 (22), product are collected It holds (1-2-4)；
(9) solenoid valve V1-1 (31) are opened, realizes the function of sampling pump emptying；
(10) solenoid valve V2-5 (40) are opened, realizes the function of elution pump emptying；
(11) above-mentioned steps (1) is returned to, carries out repetitive operation.
CN201710815322.5A 2017-09-12 2017-09-12 A kind of column position valve and its application method isolating and purifying instrument for continuous chromatography Active CN107376423B (en)

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