CN214344974U - A stirring separator for glycosaminoglycan purification - Google Patents
A stirring separator for glycosaminoglycan purification Download PDFInfo
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- CN214344974U CN214344974U CN202120213579.5U CN202120213579U CN214344974U CN 214344974 U CN214344974 U CN 214344974U CN 202120213579 U CN202120213579 U CN 202120213579U CN 214344974 U CN214344974 U CN 214344974U
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Abstract
The utility model belongs to the technical field of separation equipment, in particular to a stirring and separating device for purifying glycosaminoglycan, which comprises a tank body, wherein a refrigerating system is arranged on the periphery of the tank body, an inner cavity of the tank body is provided with a partition board, and the tank body is divided into a stirring chamber positioned on the upper part of the tank body and a separating chamber positioned on the lower part of the tank body by the partition board; the isolation plate is provided with a plurality of communicating pipes; the top of the tank body is provided with a feeding pipe and a pressurizing pipe which are communicated with the stirring chamber, and the bottom of the tank body is provided with a discharging pipe which is communicated with the separation chamber. The utility model provides a be fit for adopting acid precipitation method purification glycosaminoglycan's stirring separator, this separator simple structure, reasonable, easy operation has higher automation level to manufacturing cost has been practiced thrift.
Description
Technical Field
The utility model relates to a splitter technical field, concretely relates to a stirring separator for glycosaminoglycan purification.
Background
Glycosaminoglycan is mainly a kind of mucopolysaccharide in animal connective tissue, and exists mainly in the form of compound with protein, nucleic acid and other biological macromolecules. The glycosaminoglycan is composed of composite units of hexuronic acid and hexosamine components, and the molecules have multiple biological activities of anticoagulation, antithrombotic property, anti-inflammation, anti-tumor metastasis, immunoregulation and the like. Heparin is the most important glycosaminoglycan product, the purification process is the process of gradually reducing the load of protein and nucleic acid until reaching the standard requirement of pharmacopeia, the main purification means comprise an enzymolysis method, a salt hydrolysis method, an anion resin adsorption and gradient elution method, an acid precipitation method, an oxidation method, a low-power alcohol precipitation method, a filtration method and the like, wherein the acid precipitation method is a process for effectively removing impurities such as protein, nucleic acid and the like in glycosaminoglycan, and has the advantages of rapidness, high efficiency, less waste liquid generation, reliable quality, low production cost and the like. However, the prior glycosaminoglycan product mainly adopts an anion resin adsorption and gradient elution method in the separation and purification process, and the method has long production period and high production cost.
Therefore, a production separation device suitable for glycosaminoglycan purification by an acid precipitation method is needed, and can replace the production process of an anion resin adsorption and gradient elution method in production, so that the matching degree of the process and equipment is improved, the production period is shortened, the product quality is improved, the production cost is reduced, and the international competitiveness of glycosaminoglycan products is improved.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a be fit for adopting acid precipitation method purification glycosaminoglycan's stirring separator, for this, the utility model provides a following technical scheme:
a stirring and separating device for purifying glycosaminoglycan comprises a tank body, wherein a refrigerating system is arranged on the periphery of the tank body, an inner cavity of the tank body is provided with a partition board, and the partition board divides the tank body into a stirring chamber positioned at the upper part of the tank body and a separating chamber positioned at the lower part of the tank body; the isolation plate is provided with a plurality of communicating pipes; the top of the tank body is provided with a feeding pipe and a pressurizing pipe which are communicated with the stirring chamber, and the bottom of the tank body is provided with a discharging pipe which is communicated with the separation chamber.
A stirring mechanism and an air equalizing plate are arranged in the stirring chamber; the air equalizing plate is positioned at the upper part of the stirring chamber, the discharge hole of the feeding pipe is positioned below the air equalizing plate, and the air outlet of the pressurizing pipe is positioned above the air equalizing plate; the stirring mechanism comprises a stirring shaft and a stirring paddle arranged around the stirring shaft, and the stirring shaft penetrates through the air equalizing plate and the upper wall of the tank body and is fixedly connected with an output shaft of the driving motor.
The separation chamber is filled with diatomite, and the upper surface of the diatomite is paved with buffer cotton; and a side door is arranged on one side of the separation chamber.
The jar internal temperature sensor and the PH detector of being equipped with.
The pressurizing pipe is connected with an air booster pump.
The feed pipe, the discharge pipe and the communicating pipe are all provided with electronic switch valves, and each electronic switch valve, the temperature sensor, the PH detector, the air booster pump and the driving motor are all connected with the control panel through leads.
The utility model has the advantages of it is following:
(1) the temperature of the tank body can be monitored and controlled at any time through the temperature sensor, and the temperature can be reasonably adjusted;
(2) the separation chamber below the tank body can effectively and quickly filter the feed liquid through the filled diatomite to remove precipitates, so that the automation level of production is improved, and the product quality is improved; in addition, when the filtration is carried out, the tank body is pressurized by the air booster pump, so that the filtration and separation time is effectively shortened;
(3) due to the arrangement of the air equalizing plate, the phenomenon of uneven filtering and separation caused by uneven pressurization is effectively avoided, and the utilization efficiency of the diatomite is improved;
(4) the isolation plate is provided with a plurality of communicating pipes, each communicating pipe can be controlled to be closed through an electronic switch valve, one drainage is dispersed into a plurality of drainage, the problem of low separation efficiency caused by concentrated downward flow of all materials when the communicating pipes are opened is effectively solved, and the utilization rate of the diatomite is improved;
(5) the utility model has simple and reasonable structure and simple operation.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Referring to fig. 1, the stirring and separating device for purifying glycosaminoglycan comprises a tank body 1, wherein a refrigeration system 2 is arranged on the periphery of the tank body 1, and the refrigeration system 2 is used for adjusting the temperature in the tank body 1; the inner cavity of the tank body 1 is provided with a partition plate 3, and the partition plate 3 divides the tank body 1 into a stirring chamber 4 positioned at the upper part of the tank body 1 and a separation chamber 5 positioned at the lower part of the tank body 1; a plurality of communicating pipes 6 are arranged on the isolation plate 3; the top of the tank body 1 is provided with a feeding pipe 7 and a pressurizing pipe 8 which are communicated with the stirring chamber 4, and the bottom of the tank body is provided with a discharging pipe 9 communicated with the separation chamber 5. The partition plate 3 is provided with a plurality of communicating tubes 6, so that when the communicating tubes 6 are opened, the reaction liquid flowing downward has a plurality of ports and is dispersed into a plurality of streams of fluid, which makes the reaction liquid flowing downward more uniform.
A stirring mechanism and an air equalizing plate 10 are arranged in the stirring chamber 4; the air-equalizing plate 10 is positioned at the upper part of the stirring chamber 4, the discharge hole of the feeding pipe 7 is positioned below the air-equalizing plate 10, and the air outlet of the pressurizing pipe 8 is positioned above the air-equalizing plate 10; the stirring mechanism comprises a stirring shaft (11) and a stirring paddle (12) arranged around the stirring shaft (11), wherein the stirring shaft (11) penetrates through the air-equalizing plate (10) and the upper wall of the tank body (1) and is fixedly connected with an output shaft of a driving motor (13). The stirring mechanism is matched with the driving motor 13 to stir the reaction liquid, so that the reaction is more uniform.
The separation chamber 5 is filled with diatomite, and the upper surface of the diatomite is paved with buffer cotton 14; a side door 16 is provided at one side of the separation chamber 5. The diatomite plays a main role in filtering and separating, and the buffer cotton 14 mainly plays a role in buffering the reaction liquid left in the stirring chamber 4 so as to prevent the reaction liquid from being left to disperse the diatomite, so that the separation is not uniform, and the utilization rate of the diatomite is low. Set up side door 16 for the separating chamber, conveniently look over the in service behavior of diatomaceous earth, in time change diatomaceous earth and buffering cotton 14.
A temperature sensor 17 and a PH detector 18 are arranged in the tank body 1, the temperature sensor 17 is used for monitoring the temperature in the tank body 1 at any time and is matched with the refrigerating system 2 for use; the pH detector 18 is used to monitor the pH value in the tank 1 at any time.
The pressure pipe 8 is connected with an air booster pump, and the air booster pump is used for boosting the pressure in the tank body 1 and accelerating the flow of the reaction liquid in the tank body 1. When the air booster pump conveys air into the tank body 1, the conveyed air firstly passes through the air equalizing plate to disperse the air flow, so that the pressure in the whole tank body 1 is more uniform, and the reaction liquid flowing under the action of the pressure is more uniform.
The feed pipe 7, the discharge pipe 9 and the communicating pipe 6 are all provided with electronic switch valves 19, and each electronic switch valve 19, the temperature sensor 17, the PH detector 18, the air booster pump and the driving motor 13 are all connected with a control panel through leads.
The utility model discloses a theory of operation is: when the device is used, the electronic switch valves 19 on the communicating pipe 6 and the discharge pipe 9 are closed through the control panel, the electronic switch valve 19 of the feeding pipe 7 is opened, then the prepared crude glycosaminoglycan aqueous solution is added into the stirring chamber 4 through the feeding pipe 7, the driving motor 13 is started at the moment, hydrochloric acid is added through the feeding pipe 7 under the gradual stirring of the stirring mechanism, the hydrochloric acid is stopped when the pH detector displays the target temperature, then the stirring is continued for a period of time until the reaction is finished, and the electronic switch valve 19 of the feeding pipe 7 is closed; starting the refrigerating system 2 in the whole process from the addition of the crude glycosaminoglycan aqueous solution to the completion of stirring, and controlling the temperature of the tank body 1 to be maintained at the required temperature by matching with the temperature sensor 17; after stirring, the electronic switch valves 19 on the communicating pipe 6 and the discharging pipe 9 are opened through the control panel, the air booster pump is opened again, the tank body 1 is continuously pressurized, the acidified crude glycosaminoglycan aqueous solution flows downwards, the crude glycosaminoglycan aqueous solution passes through the separation chamber 5, and precipitates in the crude glycosaminoglycan aqueous solution are retained on the buffer cotton 14 and the diatomite through the buffering of the buffer cotton 14 and the separation and purification of the diatomite to obtain the aqueous glycosaminoglycan solution, the separated and purified aqueous glycosaminoglycan solution flows out through the discharging pipe 9, and the purified glycosaminoglycan product is obtained through the subsequent conventional process steps of alcohol precipitation, oxidation, freeze drying and the like.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. A stirring separation device for purifying glycosaminoglycan comprises a tank body (1), and is characterized in that a refrigeration system (2) is arranged on the periphery of the tank body (1), a partition plate (3) is arranged in an inner cavity of the tank body (1), and the tank body (1) is divided into a stirring chamber (4) positioned at the upper part of the tank body (1) and a separation chamber (5) positioned at the lower part of the tank body (1) by the partition plate (3); a plurality of communicating pipes (6) are arranged on the isolation plate (3); the top of the tank body (1) is provided with a feeding pipe (7) and a pressurizing pipe (8) which are communicated with the stirring chamber (4), and the bottom of the tank body is provided with a discharging pipe (9) which is communicated with the separation chamber (5).
2. The agitation separation apparatus for glycosaminoglycan purification according to claim 1, wherein the agitation mechanism and the air equalizing plate (10) are provided in the agitation chamber (4); the air-equalizing plate (10) is positioned at the upper part of the stirring chamber (4), the discharge hole of the feeding pipe (7) is positioned below the air-equalizing plate (10), and the air outlet of the pressurizing pipe (8) is positioned above the air-equalizing plate (10); the stirring mechanism comprises a stirring shaft (11) and a stirring paddle (12) arranged around the stirring shaft (11), wherein the stirring shaft (11) penetrates through the air-equalizing plate (10) and the upper wall of the tank body (1) and is fixedly connected with an output shaft of a driving motor (13).
3. The stirring separation device for purifying glycosaminoglycan according to claim 1, wherein the separation chamber (5) is filled with diatomite, and the upper surface of the diatomite is paved with buffer cotton (14); and a side door (16) is arranged on one side of the separation chamber (5).
4. The agitation separation apparatus for glycosaminoglycan purification according to claim 1, wherein a temperature sensor (17) and a PH detector (18) are provided in the tank (1).
5. The agitation separation apparatus for glycosaminoglycan purification according to claim 1, wherein the pressurization pipe (8) is connected to an air pressurization pump.
6. The agitation separation apparatus for glycosaminoglycan purification according to any one of claims 1 to 5, wherein the feed pipe (7), the discharge pipe (9) and the connection pipe (6) are provided with electronic on-off valves (19), and each of the electronic on-off valves (19), the temperature sensor (17), the pH detector (18), the air booster pump and the driving motor (13) is connected to the control panel through a wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120213579.5U CN214344974U (en) | 2021-01-26 | 2021-01-26 | A stirring separator for glycosaminoglycan purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120213579.5U CN214344974U (en) | 2021-01-26 | 2021-01-26 | A stirring separator for glycosaminoglycan purification |
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| Publication Number | Publication Date |
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| CN214344974U true CN214344974U (en) | 2021-10-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202120213579.5U Active CN214344974U (en) | 2021-01-26 | 2021-01-26 | A stirring separator for glycosaminoglycan purification |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114504962A (en) * | 2022-03-02 | 2022-05-17 | 成都蜀星饲料有限公司 | Production system of organic composite trace elements |
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2021
- 2021-01-26 CN CN202120213579.5U patent/CN214344974U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114504962A (en) * | 2022-03-02 | 2022-05-17 | 成都蜀星饲料有限公司 | Production system of organic composite trace elements |
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