CN102586088A - Sterile ultrafiltration concentration enzymic preparation fermentation broth post-extraction production line - Google Patents
Sterile ultrafiltration concentration enzymic preparation fermentation broth post-extraction production line Download PDFInfo
- Publication number
- CN102586088A CN102586088A CN2011102781613A CN201110278161A CN102586088A CN 102586088 A CN102586088 A CN 102586088A CN 2011102781613 A CN2011102781613 A CN 2011102781613A CN 201110278161 A CN201110278161 A CN 201110278161A CN 102586088 A CN102586088 A CN 102586088A
- Authority
- CN
- China
- Prior art keywords
- storage tank
- valve
- ultrafiltration
- filtration
- outlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a sterile ultrafiltration concentration enzymic preparation fermentation broth post-extraction production line, which solves the problems in the prior art that the technology is simple and crude and is subject to external bacteria pollution easily and the like. The production line is characterized in that a fermentation broth storage tank is communicated with a plate-and-frame filter press, a filter pressing clear liquid storage tank, a microfilter membrane filtering machine, a microfilteration ultrafilteration post-concentration solution storage tank and an ultrafiltration membrane filtering machine through a valve and a pump. A disinfectant circulation branch system is arranged between the filter pressing clear liquid storage tank and the microfilter membrane filtering machine. The production line utilizes sealing type filtering devices of plate-and-frame press filtering, tube membrane microfiltering and rolling membrane ultrafiltration comprehensively, fully utilizes advantages of all devices, achieves remarkable effects in the aspects of degerming and deslagging, purifying active ingredients, reducing labor intensity, saving energy, reducing emission and the like, utilizes a liquid sterilization agent to conduct thorough disinfection on pipelines, filtering membranes, storage tanks, power facilities and the like, and avoids pollution of external bacteria.
Description
Technical field:
The present invention relates to extraction system behind a kind of enzyme preparation fermented liquid, extract production line and operating procedure after relating to a kind of aseptic ultrafiltration and concentration type enzyme preparation fermented liquid or rather.
Background technology:
The back extraction production line of enzyme preparation fermented liquid; The limpid degree of zymin the finished product, the purity of effective constituent, the stability of remove the gred bacteria-eliminating efficacy, quality product have been determined; Whether also promptly finally influence product has high quality, satisfied more extensive use necessary requirement whether, whether energy-saving and emission-reduction of production process, environmental protection whether more, therefore; Strengthen Study on extraction process and exploitation after the zymin, the development of zymin industry is had important commercial value.
China's tradition zymin extraction process simple, coarse generally adopts filter press to add ultrafiltration and concentration, and product is impure many, and degerming removes solid weak effect; Product clarity is low, and simultaneously, waste liquid, waste sludge discharge are more; Be difficult to reach environmental requirement, energy consumption is big, labour intensity is high, is not suitable for industry development requirement.And it is not thorough to extract equipment disinfection, and storage tank is in open state, is subject to external living contaminants, and final product can't meet the requirement of health level, has increased finished product subsequent disposal difficulty.
Summary of the invention:
The objective of the invention is to overcome the deficiency of extractive technique behind the existing enzyme preparation fermented liquid, extract production line after a kind of novel aseptic ultrafiltration and concentration type enzyme preparation fermented liquid is provided; This production line degerming slagging-off is effective, and product clarity is high, and energy consumption is low, and ultrafiltration and concentration system sterilization is thorough, stops the secondary pollution of external assorted bacterium to finished fluid, and the finished product fluid power reaches the foodstuff sanitation-stage requirement.
The present invention includes plate-and-frame filter press etc., it is characterized in that the outlet of fermented liquid storage tank is connected with the import of plate-and-frame filter press through valve, pump, the outlet of plate-and-frame filter press is connected to press filtration clear liquid storage tank through valve, forms the sheet frame press filteration system; The outlet of press filtration clear liquid storage tank is connected with the import of micro-filtrate membrane filtration machine through valve, pump; Pump outlet in press filtration clear liquid storage tank outlet connection; Connect into a minor loop through pump, valve and the outlet of micro-filtrate membrane filtration machine trapped fluid, form the tubular type micro-filtration recycle system; The outlet of micro-filtrate membrane filtration machine connects liquid concentrator storage tank after the micro-filtration ultrafiltration through valve; The outlet of liquid concentrator storage tank is connected with the import of ultrafiltration membrance filter machine through valve, pump after the micro-filtration ultrafiltration; Export at rolling ultrafiltration membrance filter machine trapped fluid; Connect into a loop through liquid concentrator storage tank after two placed in-line valves and the micro-filtration ultrafiltration, form rolling ultrafiltration membrance filter system; Between valve that press filtration clear liquid storage tank outlet connects, pump, connect liquid disinfection liquid circulation branch road, comprise thimerosal storage tank, pure water storage tank and control corresponding valve.
The liquid concentrator storage tank also connects the degerming respiratory organ after described press filtration clear liquid storage tank, the micro-filtration ultrafiltration, gets into the filter pipe system with regulating tank internal pressure, the external grey trifle in the world bacterium of prevention.
Pump outlet in that the outlet of fermented liquid storage tank connects connects a branch road through valve and links to each other with the fermented liquid storage tank, and the plate-and-frame filter press outlet connects a branch road through valve and links to each other with the fermented liquid storage tank.
Trapped fluid outlet at the micro-filtrate membrane filtration machine connects into a middle loop through two placed in-line valves and press filtration clear liquid storage tank; Constitute a big loop through two placed in-line valves and fermented liquid storage tank.
In the trapped fluid outlet of rolling ultrafiltration membrance filter machine, connect into a finished fluid outlet through two placed in-line valves; At rolling ultrafiltration membrance filter machine purified liquor outlet, connect into waste liquid outlet through valve.
At the abhiseca place of press filtration clear liquid storage tank, connect pure water (dialysis water) branch road through valve.
At press filtration clear liquid storage tank abhiseca, connect into press filtration clear liquid storage tank through the degerming respiratory organ and breathe outlet; Liquid concentrator storage tank abhiseca after the micro-filtration ultrafiltration connects into after the micro-filtration ultrafiltration liquid concentrator storage tank through the degerming respiratory organ and breathes outlet.
Can know by technique scheme; The present invention has comprehensively adopted the sealed filtration novel process of filter press, tubular membrane micro-filtration, rolled film ultrafiltration; In conjunction with press filtration, micro-filtration, ultrafiltration apparatus characteristics; Make full use of the advantage of each equipment, obtained significant effect at aspects such as degerming slagging-off, purifying effective constituent, minimizing labour intensity, energy-saving and emission-reduction.
1, good solid-liquid separation effect is arranged after utilizing plate-and-frame filter press to form filtering layer, solid phase part liquid content is few, and loss of effective components is little, and the solid water cut is few, and the solid phase drying cost is low; Filter press can separate fermented liquid overwhelming majority solid phase, has improved the microfiltration membrane operating mode.
2, microfiltration membrane can be held back residual thalline impurity and polymer soluble material after the press filtration of fermented liquid mesoplate effectively, guarantees that product clarity is high, impure few; Micro-filtration is held back part and is returned press filtration function maximum possible recovery solid, separates liquid phase, reclaims effective constituent, simultaneously, alleviates the environmental protection pressure of discharging.
3, ultra-filtration membrane can filter small-molecule substance, holds back effective constituent, improves finished product purity; Can be according to the requirement of finished product vigor, the adjustment cycles of concentration; Ultrafiltration and concentration belongs to physical sepn, and cost is low, less energy consumption.
4, liquid disinfection liquid circulation branch road: can in conjunction with storage tank the degerming respiratory organ be installed effectively to ultrafiltration and concentration system pipeline, equipment thorough disinfection, can prevent effectively that external assorted bacterium is to the finished fluid secondary pollution.
Description of drawings:
Accompanying drawing is a structural representation of the present invention.
Among the figure, 1. fermented liquid storage tank, 2. plate-and-frame filter press, 3,7. degerming respiratory organ, 4. press filtration clear liquid storage tank, 5. micro-filtrate membrane filtration machine, 6. liquid concentrator storage tank after the micro-filtration ultrafiltration, 8. ultrafiltration membrance filter machine, 9. thimerosal storage tank, 10. pure water storage tank;
F representes valve, F1, F2 ... F20, F21; B representes pump: B1, B2, B3, B4.
Embodiment:
Below in conjunction with accompanying drawing the present invention is further described.
Shown in accompanying drawing, the present invention includes main structure of system, and the system branch structure.
About main structure of system:
The outlet of fermented liquid storage tank 1 is connected with the import of plate-and-frame filter press 2 through valve F1, pump B1, and the outlet of plate-and-frame filter press 2 is connected to press filtration clear liquid storage tank 4 through valve F2, forms the sheet frame press filteration system; The outlet of press filtration clear liquid storage tank 4 is connected with the import of micro-filtrate membrane filtration machine 5 through valve F3, pump B2, in pump B2 outlet, connects into a minor loop through pump B4, valve F9 and the outlet of micro-filtrate membrane filtration machine 5 trapped fluids, forms the tubular type micro-filtration recycle system; The outlet of micro-filtrate membrane filtration machine 5 connects liquid concentrator storage tank 6 after the micro-filtration ultrafiltration through valve F4; The outlet of liquid concentrator storage tank 6 is connected with the import of ultrafiltration membrance filter machine 8 through valve F10, pump B3 after the micro-filtration ultrafiltration; Export at rolling ultrafiltration membrance filter machine 8 trapped fluids; Connect into a loop through F14, F16 and liquid concentrator storage tank 6, form rolling ultrafiltration membrance filter system.
About the system branch structure:
(1) in pump B1 outlet, connect a branch road through F5 and link to each other with fermented liquid storage tank 1, plate-and-frame filter press 2 outlets connect a branch road through valve F6 and link to each other with fermented liquid storage tank 1.
(2) in the outlet of micro-filtrate membrane filtration machine 5 trapped fluids, connect into a middle loop through F9, F7 and press filtration clear liquid storage tank 4; Constitute a big loop through F9, F8 and fermented liquid storage tank 1.
(3), connect into a finished fluid outlet through F14, F11 in the outlet of rolling ultrafiltration membrance filter machine 8 trapped fluids; In rolling ultrafiltration membrance filter machine 8 clear liquids (waste liquid) outlet, connect into waste liquid outlet through F12.
(4), connect pure water (dialysis water) branch road through F2 at press filtration clear liquid storage tank 4 abhiseca places.
(5), connect into storage tank 4 through degerming respiratory organ 3 and breathe outlet at press filtration clear liquid storage tank 4 abhisecas; Liquid concentrator storage tank 6 abhisecas after the micro-filtration ultrafiltration connect into after the micro-filtration ultrafiltration liquid concentrator storage tank 6 through degerming respiratory organ 7 and breathe outlet; Valve F15 is installed on degerming respiratory organ 7.
(6) on the pipeline between valve F3 and the pump B2, be connected liquid disinfection liquid circulation branch road: comprise thimerosal storage tank 9, pure water storage tank 10, F17, F18, F19, F20, F21 through valve F19; F18 links to each other with pipeline between F14, the product outlet F11, and F17 links to each other with ultrafiltration and concentration waste liquid outlet valve F12 front-end pipelines; The thimerosal storage tank meets drainage valve F21 9 times, on connect F20 and pure water storage tank 10.
Operating process of the present invention:
(1), filter press operating process
Said like lower component: fermented liquid storage tank 1, valve F1, pump B1, plate-and-frame filter press 2, valve F2, valve F6, valve F5 constitute the filter press system; Wherein, valve F2 is a plate press filtration purified liquor outlet valve, and F6 is the turbid solution reverse flow valve, and F5 is the fermented liquid variable valve.
Fermented liquid through valve F1, pump B1, gets into plate-and-frame filter press under the driving of pump B1, clear liquor gets into plate press filtration clear liquid storage tank 4 through F2, accomplishes the filter press process.Wherein:
1, function as follows with the parallelly connected fermented liquid variable valve valve F5 of pump B1 outlet: 1, adjustment plate-and-frame filter press 2 work initial stage pump B1 pump into the flow size of fermented liquid, and the filtering layer that quickens plate-and-frame filter press 2 forms; Later stage, the machine internal pressure rose rapidly along with the filtering layer of plate-and-frame filter press 2 is more and more thicker, and filtration velocity slows down; Open F5, release the fermented liquid that part pump B1 pumps into, let plate-and-frame filter press 2 diaphragm cavity internal pressures slowly rise; Up to the full solid filter residue of diaphragm cavity content; Turn off F5 and B1 pump again, start pneumatics, press dry filter residue.
2, function as follows with the parallelly connected turbid solution reverse flow valve F6 of plate-and-frame filter press 2 outlets: at the 2 work initial stages of plate-and-frame filter press, filtering layer does not form or is not formed into enough thick as yet, and a large amount of solid phases see through barrier film and get into filtrating; At this moment, open F6, let filtrating be back to storage tank; After plate-and-frame filter press 2 formation enough thick filtering layer, filtrating are enough limpid, close F6, open valve F2; Plate-and-frame filter press 2 works on, and filtrating flows to clear liquid storage tank 4 through valve F2, accomplishes up to this batch filter press.
(2), tubular membrane micro-filtration operating process
Said like lower component: the pump B4 of press filtration clear liquid storage tank 4, valve F3, pump B2 and outlet parallel connection, valve F9, F7, F8, and outlet connect valve F4 etc. and constitute the tubular membrane microfiltration systems; Wherein, pump B4, valve F9, micro-filtrate membrane filtration machine 5, outlet connect the formation tubular membrane filter recycle systems such as valve F4.
Open F3, ejector priming B2, the filter press clear liquid in the press filtration clear liquid storage tank 4 pumps into the tubular membrane filter recycle system under pump B2 drives; Open F9, start recycle pump B4, open F4; The filter press clear liquid gets into micro-filtrate membrane filtration machine 5 and obtains other clear liquid of micro-filtration level under the driving of B4, and clear liquid gets into storage tank 6 through F4; Raffinate continues continuous circulating filtration under the effect of B4, and simultaneously, B2 constantly replenishes feed liquid in the micro-filtration recycle system; Along with sludge concentration progressively improves, micro-filtration recycle system internal pressure can progressively raise, when reaching certain pressure in the micro-filtration recycle system; Open valve F7, with raffinate release in the storage tank 4 with jar in the clear liquid mixed diluting, close F7 again and continue aforesaid operations; After multi-pass operations, micro-filtration filtering system internal pressure is fluctuation no longer, when being stabilized in set(ting)value; Explain that sludge concentration is consistent with the interior concentration of micro-filtration filtering system in the press filtration clear liquid storage tank 4, this moment, micro-filtration filtered near the limit; Then, open F13, in jar 4, replenish a certain amount of pure water, residue in the thinning tank is closed F13, repeats above-mentioned micro-filtration operation, up to The above results occurring; Close F7 then, open F8, residue is pressed into storage tank 1, residue is carried out filter press, accomplish the operation of tubular membrane micro-filtration this moment.
(3), rolled film ultrafiltration and concentration operating process
Said like lower component: liquid concentrator storage tank 6 after the micro-filtration ultrafiltration, F10, B3, ultrafiltration membrance filter machine 8, F14, F16, F12, F11, and degerming respiratory organ 7 constitute rolled film ultrafiltration and concentration systems.
Micro-filtration clear liquid after the micro-filtration ultrafiltration in the liquid concentrator storage tank 6 is under the driving of pump B3, through valve F10, pump B3, entering ultra-filtration membrane thickner 8; Waste liquid such as small molecules and water passes rolled film and discharges through waste liquid outlet valve F12, and liquid concentrator is liquid concentrator storage tank 6 after F14, the ultrafiltration of F16 backflow micro-filtration, and aforesaid operations moves in circles; Reach necessary requirement up to the liquid concentrator enzyme activity; Turn off F16 then, open finished product outlet valve F11, liquid concentrator is discharged to finished product storage tank through product outlet.
Degerming respiratory organ F7 plays the 6 jars of internal pressure effects of liquid concentrator storage tank after the micro-filtration ultrafiltration of regulating.
(4), ultrafiltration and concentration system sterilization sterilization flow process:
1, closes like lower valve: F3, F9, F11, F12, F20, F21; Open like lower valve: F4, F16, F14, F10, F15; Ejector priming B2, B3 are full of each equipment, pipeline up to thimerosal, then; Stop B2, B3, let medicining liquid dipping 45 minutes (the concrete time is confirmed according to the sterilization result).
2, open the sewage draining exit of liquid concentrator storage tank 6 after tube type filter membrance Microfilter 5, rolling ultra-filtration membrane ultrafilter 8, the micro-filtration ultrafiltration, thimerosal storage tank 9, lay down thimerosal, shut sewage draining exit then.
3, open valve F20, F17, F18, in thimerosal storage tank 9, fill with sterile purified water from pure water storage tank 10, shut-off valve F14 starts B2, B3 then; Be full of equipment and pipeline up to pure water, then, close F20; Stop in thimerosal storage tank 9, to inject pure water, pure water is under the effect of pump, behind the circulation flushing certain hour; Stop pump B2, B3, open sewage draining exit everywhere, lay down the cleaning waste liquid.Repeatable operation can clean up the thimerosal raffinate 2-3 time.
Claims (2)
1. extract production line behind an aseptic ultrafiltration and concentration type enzyme preparation fermented liquid, comprise plate-and-frame filter press etc., it is characterized in that:
The outlet of fermented liquid storage tank (1) is connected with the import of plate-and-frame filter press (2) through valve F1, pump B1, and the outlet of plate-and-frame filter press (2) is connected to press filtration clear liquid storage tank (4) through valve F2, forms the sheet frame press filteration system; The outlet of press filtration clear liquid storage tank (4) is connected with the import of micro-filtrate membrane filtration machine (5) through valve F3, pump B2; Export at pump B2; Connect into a minor loop through pump B4, valve F9 and the outlet of micro-filtrate membrane filtration machine (5) trapped fluid, form the tubular type micro-filtration recycle system; The outlet of micro-filtrate membrane filtration machine (5) connects liquid concentrator storage tank (6) after the micro-filtration ultrafiltration through valve F4; The outlet of liquid concentrator storage tank (6) is connected with the import of ultrafiltration membrance filter machine (8) through valve F10, pump B3 after the micro-filtration ultrafiltration; Export at rolling ultrafiltration membrance filter machine (8) trapped fluid; Connect into a loop through two placed in-line F14, F16 and micro-filtration ultrafiltration and concentration liquid storage tank (6), form rolling ultrafiltration membrance filter system; Between valve F3 that press filtration clear liquid storage tank (4) outlet connects, pump B2, connect liquid disinfection liquid circulation branch road, comprise thimerosal storage tank, pure water storage tank and control corresponding valve F17, F18, F19, F20, F21 through valve F19; F18 links to each other with pipeline between F14, the product outlet F11, and F17 links to each other with ultrafiltration and concentration waste liquid outlet valve F12 front-end pipelines; The thimerosal storage tank meets drainage valve F21 9 times, on connect F20 and pure water storage tank 10.
2. extract production line behind the aseptic ultrafiltration and concentration type enzyme preparation fermented liquid as claimed in claim 1, it is characterized in that: described press filtration clear liquid storage tank (4) connects degerming respiratory organ (3), and liquid concentrator storage tank (6) connects degerming respiratory organ (7) after the described micro-filtration ultrafiltration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110278161 CN102586088B (en) | 2011-09-18 | 2011-09-18 | Sterile ultrafiltration concentration enzymic preparation fermentation liquid post-extraction production line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110278161 CN102586088B (en) | 2011-09-18 | 2011-09-18 | Sterile ultrafiltration concentration enzymic preparation fermentation liquid post-extraction production line |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102586088A true CN102586088A (en) | 2012-07-18 |
| CN102586088B CN102586088B (en) | 2013-04-24 |
Family
ID=46475329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110278161 Expired - Fee Related CN102586088B (en) | 2011-09-18 | 2011-09-18 | Sterile ultrafiltration concentration enzymic preparation fermentation liquid post-extraction production line |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102586088B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
| CN106834086A (en) * | 2017-02-23 | 2017-06-13 | 浙江兰博生物科技股份有限公司 | A kind of separation of fermentative broth device |
| CN110129289A (en) * | 2019-05-24 | 2019-08-16 | 义马煤业集团煤生化高科技工程有限公司 | The production method of enzyme preparation in a kind of Methanol Protein fermentation liquid |
| WO2020223003A1 (en) * | 2019-04-30 | 2020-11-05 | Lefan Capsule International Inc | Method for the production of pullulan capsules |
| WO2020263519A1 (en) * | 2019-06-26 | 2020-12-30 | Lefan Capsule International Inc | Method for the production of pullulan soft capsules |
| CN117298729A (en) * | 2023-09-28 | 2023-12-29 | 湖州嘉亨实业有限公司 | Device and process for preparing and extracting anti-allergic agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857857A (en) * | 2010-05-18 | 2010-10-13 | 青岛康地恩生物科技有限公司 | Method for preparing food-grade acid pectase preparation |
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN202201898U (en) * | 2011-09-18 | 2012-04-25 | 淮北市三和诺生物工程有限责任公司 | Sterility ultrafiltration concentrated type post extraction product line for fermentation liquor of enzymic preparations |
-
2011
- 2011-09-18 CN CN 201110278161 patent/CN102586088B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857857A (en) * | 2010-05-18 | 2010-10-13 | 青岛康地恩生物科技有限公司 | Method for preparing food-grade acid pectase preparation |
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN202201898U (en) * | 2011-09-18 | 2012-04-25 | 淮北市三和诺生物工程有限责任公司 | Sterility ultrafiltration concentrated type post extraction product line for fermentation liquor of enzymic preparations |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
| CN104817452B (en) * | 2014-12-19 | 2016-09-28 | 三达膜科技（厦门）有限公司 | A kind of method extracting abscisic acid from abscisic acid fermentation liquid |
| CN106834086A (en) * | 2017-02-23 | 2017-06-13 | 浙江兰博生物科技股份有限公司 | A kind of separation of fermentative broth device |
| WO2020223003A1 (en) * | 2019-04-30 | 2020-11-05 | Lefan Capsule International Inc | Method for the production of pullulan capsules |
| US10899849B2 (en) | 2019-04-30 | 2021-01-26 | Lefan Capsule International Inc | Method for the production of pullulan capsules |
| CN113412110A (en) * | 2019-04-30 | 2021-09-17 | 力凡胶囊国际股份有限公司 | Method for producing pullulan capsule |
| CN110129289A (en) * | 2019-05-24 | 2019-08-16 | 义马煤业集团煤生化高科技工程有限公司 | The production method of enzyme preparation in a kind of Methanol Protein fermentation liquid |
| WO2020263519A1 (en) * | 2019-06-26 | 2020-12-30 | Lefan Capsule International Inc | Method for the production of pullulan soft capsules |
| US10894971B1 (en) | 2019-06-26 | 2021-01-19 | Lefan Capsule International Inc | Method for the production of pullulan soft capsules |
| CN117298729A (en) * | 2023-09-28 | 2023-12-29 | 湖州嘉亨实业有限公司 | Device and process for preparing and extracting anti-allergic agent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102586088B (en) | 2013-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102586088B (en) | Sterile ultrafiltration concentration enzymic preparation fermentation liquid post-extraction production line | |
| CN107321186B (en) | A kind of nanofiltration, the online reverse cleaning method and device of the hyperosmosis of reverse osmosis membrane | |
| AU2010334047B2 (en) | Water production system and operation method therefor | |
| CN101269999B (en) | Method for separating biological medicine fertilizer effective component from biogas fluid | |
| CN207158976U (en) | A kind of hydrogen-rich reverse osmosis water purifier | |
| CN102399687B (en) | Multifunctional post-extraction production line for enzyme preparation fermentation liquid | |
| CN102079591B (en) | Dual-membrane processing system and method for micro-polluted raw water | |
| CN202201898U (en) | Sterility ultrafiltration concentrated type post extraction product line for fermentation liquor of enzymic preparations | |
| CN106477767A (en) | Two-stage alkali cleaning formula extracts the equipment of fulvic acid in poisons in freshwater | |
| CN106315925A (en) | Device for concentrating and purifying organic matters in fresh water body and preparation method | |
| CN102399685B (en) | Finished product residual liquid recoverable post-extraction production line of enzyme preparation fermentation liquid | |
| CN111072193A (en) | Ultrafiltration mineral water production system | |
| CN202246680U (en) | Multifunctional post-extraction production line for enzyme preparation fermentation liquid | |
| CN207645925U (en) | Reverse osmose pure-water equipment | |
| CN106315956A (en) | Two-stage alkali washing type extracting equipment for organic matters dissolved in water body | |
| CN202208722U (en) | Finished product residual liquid recovery type enzyme preparation fermentation liquid post-extracting production line | |
| CN102399686A (en) | After-extraction production line for fermentation liquid of enzymic preparation | |
| CN202201899U (en) | Post extraction product line for fermentation liquor of enzymic preparations | |
| CN208279391U (en) | Nanofiltration water system | |
| CN106430711A (en) | Apparatus for extracting organic matters in fresh water by using two-stage resin | |
| CN207986707U (en) | Fluid filter system and its filter | |
| CN220149325U (en) | Novel ultrafiltration nanofiltration double-membrane water purification system | |
| CN212640066U (en) | MBR membrane bioreactor convenient to wash | |
| CN105668882B (en) | It is a kind of without preposition filer element type purifier | |
| CN206126987U (en) | RO reverse osmosis water purifier's intelligent water saving fixtures |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130424 Termination date: 20140918 |
|
| EXPY | Termination of patent right or utility model |