CN102399686A - After-extraction production line for fermentation liquid of enzymic preparation - Google Patents
After-extraction production line for fermentation liquid of enzymic preparation Download PDFInfo
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- CN102399686A CN102399686A CN2011102781632A CN201110278163A CN102399686A CN 102399686 A CN102399686 A CN 102399686A CN 2011102781632 A CN2011102781632 A CN 2011102781632A CN 201110278163 A CN201110278163 A CN 201110278163A CN 102399686 A CN102399686 A CN 102399686A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
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Abstract
The invention discloses an after-extraction production line for fermentation liquid of an enzymic preparation, and provides a complete set of after-extraction solution for the fermentation liquid of the enzymic preparation, which can be used for increasing purity of the enzymic preparation, controlling activity of enzyme liquid, and reducing discharge of waste impurities and waste water. The after-extraction production line for the fermentation liquid of the enzymic preparation is mainly characterized in that: all advantages of filter pressing of a plate frame, microfiltration of a tubular membrane and ultrafiltration of a roll type membrane in solid-liquid separation and liquid-liquid separation are utilized sufficiently; detailed structures and designs of all operation units are combined perfectly; and an obvious effect is achieved in removing bacteria and slag, purifying effective components, reducing labor intensity, saving energy, reducing emission and the like.
Description
Technical field:
The present invention relates to a kind of extraction production line, extract production line after relating to a kind of novel enzyme preparation fermented liquid or rather.
Background technology:
The back extraction production line of enzyme preparation fermented liquid; The limpid degree of zymin the finished product, the purity of effective constituent, the stability of remove the gred bacteria-eliminating efficacy, quality product have been determined; Whether also promptly finally influence product has high quality, satisfied more extensive use necessary requirement whether, whether energy-saving and emission-reduction of production process, environmental protection whether more, therefore; Strengthen the research and development of extracting production line and technology after the zymin, the development of zymin industry is had important commercial value.
China's tradition zymin extraction process simple, coarse generally adopts filter press to add ultrafiltration and concentration, and product is impure many; Degerming removes solid weak effect, and product clarity is poor, and product is difficult to reach the food grade requirement; Simultaneously, waste liquid, waste sludge discharge are more, are difficult to reach environmental requirement; Energy consumption is big, labour intensity is high, is not suitable for industry development requirement.
Summary of the invention:
The objective of the invention is to overcome the deficiency of extractive technique behind the existing enzyme preparation fermented liquid, extract production line after a kind of novel enzyme preparation fermented liquid is provided; This production line bacteria-eliminating efficacy is good, and product clarity is high, and energy consumption is low.
The present invention includes plate-and-frame filter press etc., it is characterized in that the outlet of fermented liquid storage tank is connected with the import of plate-and-frame filter press through valve, pump, the outlet of plate-and-frame filter press is connected to press filtration clear liquid storage tank through valve, forms the sheet frame press filteration system; The outlet of press filtration clear liquid storage tank is connected with the import of micro-filtrate membrane filtration machine through valve, pump; Pump outlet in press filtration clear liquid storage tank outlet connection; Connect into a minor loop through pump, valve and the outlet of micro-filtrate membrane filtration machine trapped fluid, form the tubular type micro-filtration recycle system; The outlet of micro-filtrate membrane filtration machine connects liquid concentrator storage tank after the micro-filtration ultrafiltration through valve; The outlet of liquid concentrator storage tank is connected with the import of ultrafiltration membrance filter machine through valve, pump after the micro-filtration ultrafiltration; Export at rolling ultrafiltration membrance filter machine trapped fluid; Connect into a loop through two placed in-line valves and liquid concentrator storage tank, form rolling ultrafiltration membrance filter system.
The liquid concentrator storage tank also connects respiratory organ after described press filtration clear liquid storage tank, the micro-filtration ultrafiltration, falls into the filter pipe system with regulating tank internal pressure, the external dust of prevention.
At the pump outlet that fermented liquid storage tank outlet connects, connects a valve and form a branch road and link to each other with the fermented liquid storage tank, plate-and-frame filter press exports valve of connection and forms a branch road and link to each other with the fermented liquid storage tank.
Trapped fluid outlet at the micro-filtrate membrane filtration machine connects two placed in-line valves and press filtration clear liquid storage tank and connects into a middle loop; Connect two placed in-line valves and fermented liquid storage tank and constitute a big loop.
In the trapped fluid outlet of rolling ultrafiltration membrance filter machine, connect two placed in-line valves and connect into a finished fluid outlet; At rolling ultrafiltration membrance filter machine purified liquor outlet, connect a valve and connect into waste liquid outlet.
At the abhiseca place of press filtration clear liquid storage tank, connect a valve and connect pure water (dialysis water) branch road through this valve.
At press filtration clear liquid storage tank abhiseca, connect into press filtration clear liquid storage tank through respiratory organ and breathe outlet; Liquid concentrator storage tank abhiseca after the micro-filtration ultrafiltration connects into after the micro-filtration ultrafiltration liquid concentrator storage tank through respiratory organ and breathes outlet.
Can know by technique scheme; The present invention has comprehensively adopted the sealed filtration novel process of filter press, tubular membrane micro-filtration, rolled film ultrafiltration; In conjunction with press filtration, micro-filtration, ultrafiltration apparatus characteristics; Make full use of the advantage of each equipment, obtained significant effect at aspects such as degerming slagging-off, purifying effective constituent, minimizing labour intensity, energy-saving and emission-reduction.
1, good solid-liquid separation effect is arranged after utilizing plate-and-frame filter press to form filtering layer, solid phase part liquid content is few, and loss of effective components is little, and the solid water cut is few, and the solid phase drying cost is low; Filter press can separate fermented liquid overwhelming majority solid phase, has improved the microfiltration membrane operating mode.
2, microfiltration membrane can be held back residual thalline impurity and polymer soluble material after the press filtration of fermented liquid mesoplate effectively, guarantees that product clarity is high, impure few; Micro-filtration is held back part and is returned press filtration function maximum possible recovery solid, separates liquid phase, reclaims effective constituent, simultaneously, alleviates the environmental protection pressure of discharging.
3, ultra-filtration membrane can filter small-molecule substance, holds back effective constituent, improves finished product purity; Can be according to the requirement of finished product vigor, the adjustment cycles of concentration; Ultrafiltration and concentration belongs to physical sepn, and cost is low, less energy consumption.
Description of drawings:
Accompanying drawing is a structural representation of the present invention.
Among the figure, 1. fermented liquid storage tank, 2. plate-and-frame filter press, 3,7. respiratory organ, 4. press filtration clear liquid storage tank, 5. micro-filtrate membrane filtration machine, 6. liquid concentrator storage tank after the micro-filtration ultrafiltration, 8. ultrafiltration membrance filter machine;
F representes valve, F1, F2 ... F14, F15; B representes pump: B1, B2, B3, B4.
Embodiment:
Below in conjunction with accompanying drawing the present invention is further described.
Shown in accompanying drawing, the present invention includes main structure of system, and the system branch structure.
About main structure of system:
The outlet of fermented liquid storage tank 1 is connected with the import of plate-and-frame filter press 2 through valve F1, pump B1, and the outlet of plate-and-frame filter press 2 is connected to press filtration clear liquid storage tank 4 through valve F2, forms the sheet frame press filteration system; The outlet of press filtration clear liquid storage tank 4 is connected with the import of micro-filtrate membrane filtration machine 5 through valve F3, pump B2, in pump B2 outlet, connects into a minor loop through pump B4, valve F9 and the outlet of micro-filtrate membrane filtration machine 5 trapped fluids, forms the tubular type micro-filtration recycle system; The outlet of micro-filtrate membrane filtration machine 5 connects liquid concentrator storage tank 6 after the micro-filtration ultrafiltration through valve F4; The outlet of liquid concentrator storage tank 6 is connected with the import of ultrafiltration membrance filter machine 8 through valve F10, pump B3 after the micro-filtration ultrafiltration; Export at rolling ultrafiltration membrance filter machine 8 trapped fluids; Connect into a loop through F14, F15 and liquid concentrator storage tank 6, form rolling ultrafiltration membrance filter system.
About the system branch structure:
(1) in pump B1 outlet, connect a branch road through F5 and link to each other with fermented liquid storage tank 1, plate-and-frame filter press 2 outlets connect a branch road through valve F6 and link to each other with fermented liquid storage tank 1.
(2) in the outlet of micro-filtrate membrane filtration machine 5 trapped fluids, connect into a middle loop through F9, F7 and press filtration clear liquid storage tank 4; Constitute a big loop through F9, F8 and fermented liquid storage tank 1.
(3), connect into a finished fluid outlet through F14, F11 in the outlet of rolling ultrafiltration membrance filter machine 8 trapped fluids; In rolling ultrafiltration membrance filter machine 8 clear liquids (waste liquid) outlet, connect into waste liquid outlet through F12.
(4), connect pure water (dialysis water) branch road through F13 at press filtration clear liquid storage tank 4 abhiseca places.
(5), connect into storage tank 4 through respiratory organ 3 and breathe outlet at press filtration clear liquid storage tank 4 abhisecas; Liquid concentrator storage tank 6 abhisecas after the micro-filtration ultrafiltration connect into after the micro-filtration ultrafiltration liquid concentrator storage tank 6 through respiratory organ 7 and breathe outlet.
Operating process of the present invention:
(1), filter press operating process
Said like lower component: fermented liquid storage tank 1, valve F1, pump B1, plate-and-frame filter press 2, valve F2, valve F6, valve F5 constitute the filter press system; Wherein, valve F2 is a plate press filtration purified liquor outlet valve, and F6 is the turbid solution reverse flow valve, and F5 is the fermented liquid variable valve.
Fermented liquid through valve F1, pump B1, gets into plate-and-frame filter press under the driving of pump B1, clear liquor gets into plate press filtration clear liquid storage tank 4 through F2, accomplishes the filter press process.Wherein:
1, function as follows with the parallelly connected fermented liquid variable valve valve F5 of pump B1 outlet: 1, adjustment plate-and-frame filter press 2 work initial stage pump B1 pump into the flow size of fermented liquid, and the filtering layer that quickens plate-and-frame filter press 2 forms; Later stage, the machine internal pressure rose rapidly along with the filtering layer of plate-and-frame filter press 2 is more and more thicker, and filtration velocity slows down; Open F5, release the fermented liquid that part pump B1 pumps into, let plate-and-frame filter press 2 diaphragm cavity internal pressures slowly rise; Up to the full solid filter residue of diaphragm cavity content; Turn off F5 and B1 pump again, start pneumatics, press dry filter residue.
2, function as follows with the parallelly connected turbid solution reverse flow valve F6 of plate-and-frame filter press 2 outlets: at the 2 work initial stages of plate-and-frame filter press, filtering layer does not form or is not formed into enough thick as yet, and a large amount of solid phases see through barrier film and get into filtrating; At this moment, open F6, let filtrating be back to storage tank; After plate-and-frame filter press 2 formation enough thick filtering layer, filtrating are enough limpid, close F6, open valve F2; Plate-and-frame filter press 2 works on, and filtrating flows to clear liquid storage tank 4 through valve F2, accomplishes up to this batch filter press.
(2), tubular membrane micro-filtration operating process
Said like lower component: the pump B4 of press filtration clear liquid storage tank 4, valve F3, pump B2 and outlet parallel connection, valve F9, F7, F8, and outlet connect valve F4 etc. and constitute the tubular membrane microfiltration systems; Wherein, pump B4, valve F9, micro-filtrate membrane filtration machine 5, outlet connect the formation tubular membrane filter recycle systems such as valve F4.
Open F3, ejector priming B2, the filter press clear liquid in the press filtration clear liquid storage tank 4 pumps into the tubular membrane filter recycle system under pump B2 drives; Open F9, start recycle pump B4, open F4; The filter press clear liquid gets into micro-filtrate membrane filtration machine 5 and obtains other clear liquid of micro-filtration level under the driving of B4, and clear liquid gets into storage tank 6 through F4; Raffinate continues continuous circulating filtration under the effect of B4, and simultaneously, B2 constantly replenishes feed liquid in the micro-filtration recycle system; Along with sludge concentration progressively improves, micro-filtration recycle system internal pressure can progressively raise, when reaching certain pressure in the micro-filtration recycle system; Open valve F7, with raffinate release in the storage tank 4 with jar in the clear liquid mixed diluting, close F7 again and continue aforesaid operations; After multi-pass operations, micro-filtration filtering system internal pressure is fluctuation no longer, when being stabilized in set(ting)value; Explain that sludge concentration is consistent with the interior concentration of micro-filtration filtering system in the press filtration clear liquid storage tank 4, this moment, micro-filtration filtered near the limit; Then, open F13, in jar 4, replenish a certain amount of pure water, residue in the thinning tank is closed F13, repeats above-mentioned micro-filtration operation, up to The above results occurring; Close F7 then, open F8, residue is pressed into storage tank 1, residue is carried out filter press, accomplish the operation of tubular membrane micro-filtration this moment.
(3), rolled film ultrafiltration and concentration operating process
Said like lower component: liquid concentrator storage tank 6 after the micro-filtration ultrafiltration, F10, B3, ultrafiltration membrance filter machine 8, F14, F16, F12, F11, and respiratory organ 7 constitute rolled film ultrafiltration and concentration systems.
Micro-filtration clear liquid after the micro-filtration ultrafiltration in the liquid concentrator storage tank 6 is under the driving of pump B3, through valve F10, pump B3, entering ultra-filtration membrane thickner 8; Waste liquid such as small molecules and water passes rolled film and discharges through waste liquid outlet valve F12, and liquid concentrator is liquid concentrator storage tank 6 after F14, the ultrafiltration of F16 backflow micro-filtration, and aforesaid operations moves in circles; Reach necessary requirement up to the liquid concentrator enzyme activity; Turn off F16 then, open finished product outlet valve F11, liquid concentrator is discharged to finished product storage tank through product outlet.
Respiratory organ F7 plays the 6 jars of internal pressure effects of liquid concentrator storage tank after the micro-filtration ultrafiltration of regulating.
Claims (6)
1. extract production line behind an enzyme preparation fermented liquid, comprise plate-and-frame filter press etc., it is characterized in that:
The outlet of fermented liquid storage tank (1) is connected with the import of plate-and-frame filter press (2) through valve F1, pump B1, and the outlet of plate-and-frame filter press (2) is connected to press filtration clear liquid storage tank (4) through valve F2, forms the sheet frame press filteration system; The outlet of press filtration clear liquid storage tank (4) is connected with the import of micro-filtrate membrane filtration machine (5) through valve F3, pump B2; Export at pump B2; Connect into a minor loop through pump B4, valve F9 and the outlet of micro-filtrate membrane filtration machine (5) trapped fluid, form the tubular type micro-filtration recycle system; The outlet of micro-filtrate membrane filtration machine (5) connects liquid concentrator storage tank (6) after the micro-filtration ultrafiltration through valve F4; The outlet of liquid concentrator storage tank (6) is connected with the import of ultrafiltration membrance filter machine (8) through valve F10, pump B3 after the micro-filtration ultrafiltration; Export at rolling ultrafiltration membrance filter machine (8) trapped fluid; Connect into a loop through two placed in-line F14, F15 and micro-filtration ultrafiltration and concentration liquid storage tank (6), form rolling ultrafiltration membrance filter system.
2. extract production line behind the enzyme preparation fermented liquid as claimed in claim 1, it is characterized in that:, connect into press filtration clear liquid storage tank (4) through respiratory organ (3) and breathe outlet at press filtration clear liquid storage tank (4) abhiseca; Liquid concentrator storage tank (6) abhiseca after the micro-filtration ultrafiltration connects into after the micro-filtration ultrafiltration liquid concentrator storage tank (6) through respiratory organ (7) and breathes outlet.
3. extract production line behind the enzyme preparation fermented liquid as claimed in claim 1, it is characterized in that:
In pump B1 outlet, connection valve F5 forms a branch road and links to each other with fermented liquid storage tank (1), and plate-and-frame filter press (2) outlet connects another branch road of valve F6 formation and links to each other with fermented liquid storage tank (1).
4. extract production line behind the enzyme preparation fermented liquid as claimed in claim 1; It is characterized in that: export at micro-filtrate membrane filtration machine (5) trapped fluid; Connect valve F9, F7 and press filtration clear liquid storage tank (4) and connect into a middle loop, connect F9, F8 and fermented liquid storage tank (1) and constitute a big loop.
5. extract production line behind the enzyme preparation fermented liquid as claimed in claim 1, it is characterized in that: in the outlet of rolling ultrafiltration membrance filter machine (8) trapped fluid, connection F14, F11 connect into a finished fluid outlet in rolling; At rolling ultrafiltration membrance filter machine (8) purified liquor outlet, connect F12 and connect into waste liquid outlet.
6. extract production line behind the enzyme preparation fermented liquid as claimed in claim 1, it is characterized in that: the abhiseca place is provided with F13 at press filtration clear liquid storage tank (4), and connects the pure water branch road through F13.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110278163 CN102399686B (en) | 2011-09-18 | 2011-09-18 | After-extraction production line for fermentation liquid of enzymic preparation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110278163 CN102399686B (en) | 2011-09-18 | 2011-09-18 | After-extraction production line for fermentation liquid of enzymic preparation |
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| CN102399686A true CN102399686A (en) | 2012-04-04 |
| CN102399686B CN102399686B (en) | 2013-02-27 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
| CN106834086A (en) * | 2017-02-23 | 2017-06-13 | 浙江兰博生物科技股份有限公司 | A kind of separation of fermentative broth device |
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| CN101857857A (en) * | 2010-05-18 | 2010-10-13 | 青岛康地恩生物科技有限公司 | Method for preparing food-grade acid pectase preparation |
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN102399687A (en) * | 2011-09-18 | 2012-04-04 | 淮北市三和诺生物工程有限责任公司 | Multifunctional post-extraction production line for enzyme preparation fermentation liquid |
| CN202201899U (en) * | 2011-09-18 | 2012-04-25 | 淮北市三和诺生物工程有限责任公司 | Post extraction product line for fermentation liquor of enzymic preparations |
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2011
- 2011-09-18 CN CN 201110278163 patent/CN102399686B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857857A (en) * | 2010-05-18 | 2010-10-13 | 青岛康地恩生物科技有限公司 | Method for preparing food-grade acid pectase preparation |
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN102399687A (en) * | 2011-09-18 | 2012-04-04 | 淮北市三和诺生物工程有限责任公司 | Multifunctional post-extraction production line for enzyme preparation fermentation liquid |
| CN202201899U (en) * | 2011-09-18 | 2012-04-25 | 淮北市三和诺生物工程有限责任公司 | Post extraction product line for fermentation liquor of enzymic preparations |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
| CN104817452B (en) * | 2014-12-19 | 2016-09-28 | 三达膜科技（厦门）有限公司 | A kind of method extracting abscisic acid from abscisic acid fermentation liquid |
| CN106834086A (en) * | 2017-02-23 | 2017-06-13 | 浙江兰博生物科技股份有限公司 | A kind of separation of fermentative broth device |
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