CN212102647U - L-citrulline extraction element in seed melon - Google Patents
L-citrulline extraction element in seed melon Download PDFInfo
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- CN212102647U CN212102647U CN202020496805.0U CN202020496805U CN212102647U CN 212102647 U CN212102647 U CN 212102647U CN 202020496805 U CN202020496805 U CN 202020496805U CN 212102647 U CN212102647 U CN 212102647U
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- citrulline
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- filter
- enzymolysis
- ion exchange
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Abstract
The utility model discloses a L-citrulline extraction element in seed melon, including the breaker that links to each other in proper order, the enzymolysis tank, draw the jar, centrifuge, a filter, the ultrafilter, reverse osmosis machine, ion exchange resin, macroporous adsorbent resin, decompression concentration crystallizer, put into the breaker with seed melon and carry out the breakage, carry the thick liquid to the enzymolysis tank, add a certain amount of pectinase and cellulase, and keep warm to the material, the thick liquid after the enzymolysis is sent into and is drawn jar and carry out the ethanol extraction, and insert ultrasonic generrator, the extract gets into centrifuge and carries out centrifugal separation, supernatant after the centrifugation is carried to the filter and is filtered, clear liquid after the filtration inputs the ultrafilter and carries out the ultrafiltration, it carries out the primary concentration again to get into reverse osmosis machine, remove macromolecular substance, permeate liquid passes through ion exchange resin again and adsorbs the elution to citrulline, then carry out the decoloration through macroporous adsorbent resin with the eluant, and (4) after decoloring, introducing the mixture into a reduced pressure concentration crystallization tank for concentration crystallization to obtain natural L-citrulline crystals.
Description
Technical Field
The utility model relates to a L-citrulline draws technical field in the seed melon, especially relates to a L-citrulline extraction element in seed melon.
Background
L-citrulline is an amino acid, and can improve immunity, help to recover fatigue and stress, maintain normal levels of blood glucose and cholesterol, maintain joint movement function, maintain healthy lung function, improve brain definition, etc.
Chinese patent application publication No. CN108822001A discloses a method for extracting citrulline from watermelons, which comprises the steps of raw material pretreatment, ultrasonic-enzymolysis auxiliary solvent extraction and purification; the purification step comprises microbial fermentation, ion exchange resin purification, macroporous adsorption resin decoloration, crystallization and recrystallization. Chinese patent application with publication number CN105924371A discloses an extraction method of L-citrulline in seed melons and a preparation method of effervescent tablets, wherein the method comprises the steps of preparing ultrafiltrate, performing column elution, reduced pressure distillation and crystallization, and also discloses application of the L-citrulline extracted by the method. Chinese patent application publication No. CN101372465B discloses an industrial method for extracting natural L-citrulline from plant tissues such as watermelon or watermelon, etc., which takes watermelon, watermelon or their stems and leaves as raw materials, and the process is as follows: firstly, cleaning raw materials, crushing, separating watermelon peel and watermelon pulp, and juicing respectively; mixing the obtained juices, and removing insoluble substances and macromolecular substances by microfiltration and ultrafiltration; then reverse osmosis and nanofiltration are carried out for filtration; adjusting the pH value of the permeate, and introducing into a macroporous strong-acid cation exchange resin column; then ammonia water is used as an eluant for isocratic elution; and distilling the eluent under reduced pressure to remove ammonia, decoloring, concentrating and crystallizing to obtain a white crystal product of the natural L-citrulline extracted from the watermelons. However, the methods for extracting L-citrulline all have the problem that materials are easy to block ion exchange resins or microfiltration columns and microfiltration membranes.
Therefore, how to change the current situation that materials are easy to block ion exchange resins or microfiltration columns and microfiltration membranes in the process of extracting L-citrulline in the prior art becomes a problem to be solved by the technical personnel in the field.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a L-citrulline extraction element in seed melon to solve the problem that above-mentioned prior art exists, avoid the ion exchange resin to block up, improve L-citrulline and draw work efficiency.
In order to achieve the above object, the utility model provides a following scheme: the utility model provides an L-citrulline extraction device in seed melon, which comprises a crusher, an enzymolysis tank, an extraction tank, a centrifuge, a filter, an ultrafilter, an anti-osmosis machine, ion exchange resin, macroporous adsorption resin and a decompression concentration crystallizing tank which are connected in sequence, wherein the crusher can crush materials, a discharge port of the crusher is communicated with a feed port of the enzymolysis tank, the enzymolysis tank can preserve heat of the materials, the extraction tank is connected with an ultrasonic generator, a discharge port of the extraction tank is connected with a feed port of the centrifuge, the centrifuge can centrifugally separate the materials, a discharge port of the centrifuge is connected with the feed port of the filter, the filter can filter the materials, a discharge port of the filter is connected with the feed port of the ultrafilter, the ultrafilter can ultrafiltrate the materials, the discharge gate of ultrafilter with reverse osmosis machine links to each other, reverse osmosis machine can concentrate the material, reverse osmosis machine's discharge gate with ion exchange resin's links to each other, ion exchange resin can adsorb the elution to the material, ion exchange resin with macroporous adsorption resin links to each other, macroporous adsorption resin can decolor the material, macroporous adsorption resin with the concentrated crystallizer of decompression links to each other, the concentrated crystallizer of decompression can carry out concentrated crystallization to the material.
Preferably, the jar body of enzymolysis tank is bilayer structure, the enzymolysis tank includes the inner tank body and the outer jar of body, the outer jar of body suit in the outside of the inner tank body, the inner tank body with have the clearance between the outer jar of body and form the heat preservation cavity, the heat preservation cavity is linked together with the external heat source, the external heat source can to carry the heat preservation medium in the heat preservation cavity, set up control flap between heat preservation cavity and the external heat source.
Preferably, the enzymolysis tank sets up the observation window, the observation window runs through the inner tank body the outer tank body, the observation window respectively with the inner tank body the outer tank body links to each other, the observation window with between the inner tank body with all set up sealing element between the observation window, the observation window is made by transparent material.
Preferably, the filter is a bag filter.
Preferably, the number of the filters is three, and three filters are arranged in series.
Preferably, the breaker the enzymolysis tank draw the jar centrifuge the filter the ultrafilter the reverse osmosis machine ion exchange resin macroporous adsorption resin the concentrated crystallizer of decompression all links to each other through connecting line, connecting line is connected with the delivery pump, the delivery pump can carry the material.
The utility model discloses for prior art gain following technological effect: the utility model discloses a L-citrulline extraction element in seed melon, including the breaker that links to each other in proper order, the enzymolysis tank, draw the jar, centrifuge, the filter, the ultrafilter, anti-infiltration machine, ion exchange resin, macroporous adsorption resin, decompression concentration crystallizer, the breaker can be broken the material, the discharge gate of breaker is linked together with the pan feeding mouth of enzymolysis tank, enzymolysis tank can keep warm to the material, draw jar and be connected with ultrasonic generrator, the discharge gate of drawing the jar links to each other with the pan feeding mouth of centrifuge, centrifuge can carry out centrifugal separation to the material, centrifuge's discharge gate links to each other with the pan feeding mouth of filter, the filter can filter the material, the discharge gate of filter links to each other with ultrafiltration machine's pan feeding mouth, ultrafiltration machine can carry out the ultrafiltration to the material, ultrafiltration machine's discharge gate links to anti-infiltration machine, anti-infiltration machine can concentrate the material, the discharge gate of reverse osmosis machine links to each other with ion exchange resin, and ion exchange resin can adsorb the elution to the material, and ion exchange resin links to each other with macroporous adsorption resin, and macroporous adsorption resin can carry out the decoloration to the material, and macroporous adsorption resin links to each other with the concentrated crystallizer of decompression, and the concentrated crystallizer of decompression can carry out concentrated crystallization to the material. The utility model discloses a L-citrulline extraction element during operation in the seed melon, put into the breaker with the seed melon and carry out the breakage, carry the thick liquid to the enzymolysis tank, add a quantitative pectinase and cellulase, and keep warm to the material, the thick liquid after the enzymolysis is sent into and is drawed ethanol in the extraction tank, and access ultrasonic generrator, the extract gets into centrifuge and carries out centrifugal separation, supernatant after the centrifugation is carried to the filter and is filtered, clear liquid input ultrafilter after the filtration carries out the ultrafiltration, get into anti-infiltration machine again and carry out preliminary concentration, remove macromolecular substance, the permeate liquid adsorbs the elution through ion exchange resin to citrulline, then carry out the decoloration with the eluant through macroporous adsorption resin, let in the concentrated crystallizer of decompression after the decoloration and carry out the concentrated crystallization, obtain natural L-citrulline crystal. The utility model discloses a L-citrulline extraction element in seed melon sets up centrifuge and filter, carries out centrifugal separation and filtration to the material, can effectively avoid ion exchange resin to block up, improves L-citrulline extraction efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor.
FIG. 1 is a schematic diagram of the device for extracting L-citrulline from seed melon of the present invention;
wherein, 1 is the breaker, 2 is the enzymolysis tank, 201 is the heat preservation cavity, 202 is the observation window, 3 is the extraction jar, 4 is ultrasonic generator, 5 is centrifuge, 6 is the filter, 7 is the ultrafilter, 8 is reverse osmosis machine, 9 is ion exchange resin, 10 is macroporous adsorbent resin, 11 is the concentrated crystallizer of decompression.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model aims at providing a L-citrulline extraction element in seed melon to solve the problem that above-mentioned prior art exists, avoid the ion exchange resin to block up, improve L-citrulline and draw work efficiency.
In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.
Referring to fig. 1, fig. 1 is a schematic diagram of an L-citrulline extraction device in seed melon according to the present invention.
The utility model provides a L-citrulline extraction element in seed melon, including consecutive breaker 1, enzymolysis tank 2, draw jar 3, centrifuge 5, filter 6, ultrafilter 7, reverse osmosis machine 8, ion exchange resin 9, macroporous adsorbent resin 10, decompression concentration crystallizer 11, breaker 1 can carry out the breakage to the material, the discharge gate of breaker 1 is linked together with the pan feeding mouth of enzymolysis tank 2, enzymolysis tank 2 can keep warm to the material, draw jar 3 and be connected with ultrasonic generator 4, the discharge gate of draw jar 3 links to each other with the pan feeding mouth of centrifuge 5, centrifuge 5 can carry out centrifugal separation to the material, the discharge gate of centrifuge 5 links to each other with the pan feeding mouth of filter 6, filter 6 can filter the material, the discharge gate of filter 6 links to each other with the pan feeding mouth of ultrafilter 7, ultrafilter 7 can carry out the ultrafiltration to the material, the discharge gate of ultrafilter 7 links to each other with reverse osmosis machine 8, reverse osmosis machine 8 can be concentrated the material, reverse osmosis machine 8's discharge gate links to each other with ion exchange resin 9, ion exchange resin 9 can adsorb the elution to the material, ion exchange resin 9 links to each other with macroporous adsorbent resin 10, macroporous adsorbent resin 10 can decolor the material, macroporous adsorbent resin 10 links to each other with concentrated crystallizer of decompression 11, concentrated crystallizer of decompression 11 can carry out concentrated crystallization to the material.
When the L-citrulline extraction device in seed melons works, seed melons are put into the crusher 1 to be crushed, slurry is conveyed to the enzymolysis tank 2, a certain amount of pectinase and cellulase are added, and the materials are insulated, the slurry after enzymolysis is sent into an extraction tank 3 for ethanol extraction, and then is connected into an ultrasonic generator 4, the extract is sent into a centrifuge 5 for centrifugal separation, the centrifuged supernatant is sent to a filter 6 for filtration, the filtered supernatant is sent into an ultrafiltration machine 7 for ultrafiltration, then is sent into an anti-osmosis machine 8 for preliminary concentration, macromolecular substances are removed, the permeation solution is adsorbed and eluted by citrulline through ion exchange resin 9, then, the eluent is decolorized by macroporous adsorption resin 10, and then is introduced into a decompression concentration crystallization tank 11 for concentration and crystallization, so that natural L-citrulline crystals are obtained. The utility model discloses a L-citrulline extraction element in seed melon sets up centrifuge 5 and filter 6, carries out centrifugal separation and filtration to the material, can effectively avoid 9 clogs up of ion exchange resin, improves L-citrulline extraction efficiency.
Specifically, the tank body of the enzymolysis tank 2 is of a double-layer structure, the enzymolysis tank 2 comprises an inner tank body and an outer tank body, the outer tank body is sleeved outside the inner tank body, a gap is formed between the inner tank body and the outer tank body, a heat preservation cavity 201 is formed, the heat preservation cavity 201 is communicated with an external heat source, the external heat source can convey heat preservation media into the heat preservation cavity 201, and a control valve is arranged between the heat preservation cavity 201 and the external heat source. In the L-citrulline extraction process, 45 ℃ hot water is introduced into the heat-insulating cavity 201 from an external heat source to insulate the materials, and in the specific embodiment, the tank body of the enzymolysis tank 2 is made of stainless steel, so that the heat conductivity is good, the enzymolysis tank is not easy to corrode, and the stability is high. In addition, in order to control the flow of the materials, control valves are arranged among all the components.
In order to facilitate observation of the material state in the enzymolysis tank 2, the enzymolysis tank 2 is provided with an observation window 202, the observation window 202 penetrates through the inner tank body and the outer tank body, the observation window 202 is respectively connected with the inner tank body and the outer tank body, between the observation window 202 and the inner tank body, the material condition in the enzymolysis tank 2 can be observed through the observation window 202, a sealing element is arranged between the inner tank body and the observation window 202, leakage is avoided, the observation window 202 is made of a transparent material, what needs to be described here is that the transparent material is a conventional means of a material in the prior art, and the description is omitted here.
More specifically, the filter 6 is a bag filter, the supernatant fluid centrifugally separated by the centrifuge 5 enters the bag filter, and the filtered supernatant fluid enters the ultrafilter 7, so that the bag filter has high filtering precision and long service life.
It should be emphasized that the number of the filters 6 is three, three filters 6 are arranged in series, and a three-stage filter 6 is arranged to filter the centrifuged supernatant, thereby effectively avoiding the blockage of the ion exchange resin 9.
Further, breaker 1, enzymolysis tank 2, draw jar 3, centrifuge 5, filter 6, ultrafilter 7, reverse osmosis machine 8, ion exchange resin 9, macroporous adsorbent resin 10, decompression concentration crystallizer 11 all link to each other through the connecting line, and the connecting line is connected with the delivery pump, and the delivery pump can carry the material, sets up the delivery pump and can ensure that the material is carried smoothly, improves material conveying efficiency, prevents the material refluence, improves production efficiency.
When the L-citrulline extraction device in seed melons works, seed melons are put into the crusher 1 to be crushed, slurry is conveyed to the enzymolysis tank 2, a certain amount of pectinase and cellulase are added, and the materials are insulated, the serous fluid after enzymolysis is pumped into an extraction tank 3 for ethanol extraction, and is connected into an ultrasonic generator 4, the extracting solution is pumped into a centrifuge 5 for centrifugal separation, the supernatant fluid after centrifugation is pumped into a filter 6 for filtration, the clear fluid after filtration is pumped into an ultrafiltration machine 7 for ultrafiltration, and is pumped into an anti-osmosis machine 8 for preliminary concentration, macromolecular substances are removed, the permeation solution is used for adsorbing and eluting citrulline through ion exchange resin 9, then, the eluent is decolorized by macroporous adsorption resin 10, and then is introduced into a decompression concentration crystallization tank 11 for concentration and crystallization, so that natural L-citrulline crystals are obtained. The utility model discloses a L-citrulline extraction element in seed melon sets up centrifuge 5 and tertiary filter 6, carries out centrifugal separation and filtration to the material, can effectively avoid 9 clogs up of ion exchange resin, improves L-citrulline extraction efficiency.
The utility model discloses a concrete example is applied to explain the principle and the implementation mode of the utility model, and the explanation of the above example is only used to help understand the method and the core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present specification should not be construed as a limitation of the present invention.
Claims (6)
1. The utility model provides a L-citrulline extraction element in seed melon which characterized in that: comprises a crusher, an enzymolysis tank, an extraction tank, a centrifuge, a filter, an ultrafilter, an anti-osmosis machine, ion exchange resin, macroporous adsorption resin and a decompression concentration crystallizing tank which are connected in sequence, wherein the crusher can crush materials, a discharge port of the crusher is communicated with a feed inlet of the enzymolysis tank, the enzymolysis tank can preserve heat of the materials, the extraction tank is connected with an ultrasonic generator, a discharge port of the extraction tank is connected with a feed inlet of the centrifuge, the centrifuge can carry out centrifugal separation on the materials, the discharge port of the centrifuge is connected with the feed inlet of the filter, the filter can filter the materials, the discharge port of the filter is connected with the feed inlet of the ultrafilter, the ultrafilter can carry out ultrafiltration on the materials, the discharge port of the ultrafilter is connected with the anti-osmosis machine, the reverse osmosis machine can concentrate the material, the discharge gate of reverse osmosis machine with ion exchange resin's linking to each other, ion exchange resin can adsorb the elution to the material, ion exchange resin with macroporous adsorption resin links to each other, macroporous adsorption resin can decolor the material, macroporous adsorption resin with decompression concentration crystallizer links to each other, decompression concentration crystallizer can carry out concentrated crystallization to the material.
2. The device for extracting L-citrulline from seed melons according to claim 1, wherein: the tank body of the enzymolysis tank is of a double-layer structure, the enzymolysis tank comprises an inner tank body and an outer tank body, the outer tank body is sleeved outside the inner tank body, a gap is formed between the inner tank body and the outer tank body, a heat preservation cavity is formed between the inner tank body and the outer tank body, the heat preservation cavity is communicated with an external heat source, the external heat source can convey heat preservation media into the heat preservation cavity, and a control valve is arranged between the heat preservation cavity and the external heat source.
3. The device for extracting L-citrulline from seed melons according to claim 2, wherein: the enzymolysis tank sets up the observation window, the observation window runs through the inner tank body the outer tank body, the observation window respectively with the inner tank body the outer tank body links to each other, the observation window with between the inner tank body with all set up sealing element between the observation window, the observation window is made by transparent material.
4. The device for extracting L-citrulline from seed melons according to claim 1, wherein: the filter is a bag filter.
5. The device for extracting L-citrulline from seed melons according to claim 4, wherein: the number of the filters is three, and the three filters are arranged in series.
6. The device for extracting L-citrulline from seed melons according to claim 1, wherein: the breaker enzymolysis tank draw the jar centrifuge the filter ultrafilter reverse osmosis machine ion exchange resin macroporous adsorption resin the concentrated crystallizer of decompression all links to each other through connecting line, connecting line is connected with the delivery pump, the delivery pump can carry the material.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115054945A (en) * | 2022-07-25 | 2022-09-16 | 南京百益恒安生物科技有限公司 | Preparation device and method of dendrobium officinale polysaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115054945A (en) * | 2022-07-25 | 2022-09-16 | 南京百益恒安生物科技有限公司 | Preparation device and method of dendrobium officinale polysaccharide |
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