CN107376423A - A kind of post position valve and its application method that instrument is isolated and purified for continuous chromatography - Google Patents
A kind of post position valve and its application method that instrument is isolated and purified for continuous chromatography Download PDFInfo
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- CN107376423A CN107376423A CN201710815322.5A CN201710815322A CN107376423A CN 107376423 A CN107376423 A CN 107376423A CN 201710815322 A CN201710815322 A CN 201710815322A CN 107376423 A CN107376423 A CN 107376423A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K51/00—Other details not peculiar to particular types of valves or cut-off apparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/16—Injection
- G01N30/20—Injection using a sampling valve
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/16—Injection
- G01N30/20—Injection using a sampling valve
- G01N2030/201—Injection using a sampling valve multiport valves, i.e. having more than two ports
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
A kind of post position valve and its application method that instrument is isolated and purified for continuous chromatography, belong to chemical industry, bio-pharmaceuticals equipment technical field, including the valve plate base being made up of long side valve plate, short side valve plate and top valve plate, lower valve plates, and it is arranged on the sampling pump of valve plate chassis outer side, elution pump, 4 chromatographic columns, the top valve plate of valve plate base is provided with 26 structure identical two-position two-way solenoid valves, and 26 two-position two-way solenoid valves form front-seat solenoid valve block, middle solenoid valve block and heel row solenoid valve block；The connection stream of post position valve is provided with inside valve plate pedestal.Structure novelty of the present invention is compact, Method And Principle is clear, using 4 column systems and valve board structure, flow pipe is set inside valve plate, and loading does not stop, and saves the batch processed time, filler utilization rate is high, effectively sample can be avoided to pass, save packing volume, saved the batch processed time and shorten the production time and play advantageous effect.
Description
Technical field
The invention belongs to chemical industry, bio-pharmaceuticals equipment technical field, is related to a kind of chromatographic isolation equipment and its application method,
Particularly relate to a kind of post position valve and its application method that instrument is isolated and purified for continuous chromatography.
Background technology
China possesses chemical composition of Chinese materia medica the hugest and natural drug structural research troop, is mainly engaged in natural products
Extraction separation and Structural Identification research, extensive and economy the purifying of protein is increasingly becoming biotechnology and pharmacy work
Major issue in industry, almost more than 80% work is all to carry out the separation of native compound, and this research field seems
As labor-intensive manual labor, native compound is isolated and purified as natural drug initiative or lead compound hair
Existing bottleneck, substantial amounts of time and efforts, which is wasted in, to be isolated and purified；And modern automation isolation technics equipment rapidly and efficiently is still
It is so the key of breakthrough bottleneck.Therefore combination application chromatogram new technology, the competitions of various fast separation device equipment is designed also just
Very fierce, many equipment and technical method occur has a lot, but to completely solving a kind of height of various composition in medicinal plant
Effect quick separating prepares purifier apparatus, actually rare, and complicated various in face of traditional Chinese medicine ingredients structure, and effect is preferably just more
It is few.
Typical purge process includes differential centrifugation and filtration step and at least three kinds of chromatographic separation technologies, such as affine color
Spectrometry (AC), gel permeation chromatography (GPC), ion-exchange chromatography (IEC), hydrophobic interaction chromatography (HIC), reverse-phase chromatography
Method (RPC) and normal phase chromatography (NPC).Simple batch-type chromatographic technology is widely used in commercial Application, but
Because processing time length and operation cost are high (such as quantity of solvent is big, resin and expensive hardware), this technology is very expensive, this skill
Art is also sensitive to service condition (such as product titre, residence time and charging rate).Comparatively speaking, continuous chromatography isolates and purifies
The performance of instrument seems more prominent, but existing continuous chromatography isolates and purifies instrument equipment and forms complicated, cost of manufacture height, right
Complex compound mixed system disengaging time is long, packing volume is big, the utilization ratio of filler is not high, and production efficiency cannot be effective
Lifting.
The content of the invention
The purpose of the present invention is to isolate and purify equipment for above-mentioned existing continuous chromatography to form complicated, cost of manufacture height, right
Complex compound mixed system disengaging time is long, packing volume is big, the utilization ratio of filler is not high, and production efficiency cannot be effective
The deficiencies of lifting, a kind of post position valve and its application method that instrument is isolated and purified for continuous chromatography is proposed, can using valve board structure
Effectively avoid sample from passing, it is saved packing volume to improving filler utilization ratio, is saved the batch processed time and shorten and give birth to
The production time plays advantageous effect.
The technical scheme is that：A kind of post position valve that instrument is isolated and purified for continuous chromatography, including valve plate base, with
And it is arranged on the sampling pump of valve plate chassis outer side, elution pump, chromatographic column 1, chromatographic column 2, chromatographic column 3 and chromatographic column 4；Its feature exists
In：The top valve plate of the valve plate base is provided with 26 structure identical two-position two-way solenoid valves, 26 bi-bit bi-pass
Magnetic valve forms front-seat solenoid valve block, middle solenoid valve block and heel row solenoid valve block；
The front-seat solenoid valve block by magnetic valve V5-1, magnetic valve V5-2, magnetic valve V5-3, magnetic valve V5-4, magnetic valve V3-1,
Magnetic valve V3-2, magnetic valve V3-3 and magnetic valve V3-4 are formed；
The middle solenoid valve block by magnetic valve V1-1, magnetic valve V1-2, magnetic valve V1-3, magnetic valve V1-4, magnetic valve V1-5,
Magnetic valve V2-1, magnetic valve V2-2, magnetic valve V2-3, magnetic valve V2-4 and magnetic valve V2-5 are formed；
The heel row solenoid valve block by magnetic valve V6-1, magnetic valve V6-2, magnetic valve V6-3, magnetic valve V6-4, magnetic valve V4-1,
Magnetic valve V4-2, magnetic valve V4-3 and magnetic valve V4-4 are formed；
Be provided with the connection stream of post position valve inside the valve plate pedestal, left short side valve plate be provided with the head bore of post 1, the tail hole of post 1,
The head bore of post 2, the tail hole of post 2, the head bore of post 3, the head bore of post 4, elution pump connecting hole and product collecting terminal；On right short side valve plate
Provided with the tail hole of post 4, sampling pump connecting hole, waste collection end and the tail end hole of post 3；The head bore of post 1 and the tail hole of post 1 and institute
State the headtotail of chromatographic column 1, the head bore of post 2 and the tail hole of post 2 and the headtotail of chromatographic column 2, the head bore of post 3
With the tail hole of post 3 and the headtotail of chromatographic column 3, the head bore of post 4 and the tail hole of post 4 and the chromatographic column 4 phase end to end
Connect, the elution connecting hole connects with the elution pump, and the sampling pump connecting hole connects with the sampling pump；
The connection stream of post position valve forms as follows：
(1) the sampling pump connecting hole and the magnetic valve V1-1, magnetic valve V1-2, magnetic valve V1-3, magnetic valve V1-4, electromagnetism
Valve V1-5 is formed and is connected in parallel；Wherein, the magnetic valve V1-1 is connected with the waste collection end；The magnetic valve V1-2 and electricity
The product collecting terminal is connected to after magnet valve V5-1, magnetic valve V5-2 series connection；The magnetic valve V1-3 and magnetic valve V4-1, electromagnetism
The product collecting terminal is connected to after valve V4-2 series connection；After the magnetic valve V1-4 connects with magnetic valve V3-1, magnetic valve V3-2
It is connected to the product collecting terminal；The magnetic valve V1-5 is connected to the production after being connected with magnetic valve V6-1, magnetic valve V6-2
Product collecting terminal；
(2) the elution pump connecting hole and the magnetic valve V2-1, magnetic valve V2-2, magnetic valve V2-3, magnetic valve V2-4, electromagnetism
Valve V2-5 is formed and is connected in parallel；Wherein, the magnetic valve V2-1 is connected to described after being connected with magnetic valve V4-3, magnetic valve V4-4
Waste collection end；The magnetic valve V2-2 is connected to the waste collection end after being connected with magnetic valve V5-3, magnetic valve V5-4；Institute
State after magnetic valve V2-3 connects with magnetic valve V6-3, magnetic valve V6-4 and be connected to the waste collection end；The magnetic valve V2-4
The waste collection end is connected to after being connected with magnetic valve V3-3, magnetic valve V3-4；The magnetic valve V2-5 is connected to the production
Product collecting terminal；
(3) head bore of post 1 and magnetic valve V1-5, magnetic valve the V2-1 port of export are connected in parallel；The head bore of post 2 and electricity
Magnet valve V1-4, the magnetic valve V2-2 port of export are connected in parallel；The head bore of post 3 and magnetic valve V1-3, magnetic valve V2-3 go out
Mouth end is connected in parallel；The head bore of post 4 and magnetic valve V1-2, magnetic valve the V2-4 port of export are connected in parallel；
(4) tail hole of post 1 forms phase in parallel with magnetic valve V3-1, magnetic valve V3-2, magnetic valve V3-3, magnetic valve V3-4
Connect；The tail hole of post 2 is formed and is connected in parallel with magnetic valve V4-1, magnetic valve V4-2, magnetic valve V4-3, magnetic valve V4-4；Institute
The tail hole of post 3 is stated to be formed and be connected in parallel with magnetic valve V5-1, magnetic valve V5-2, magnetic valve V5-3, magnetic valve V5-4；The post 4
Tail hole is formed and is connected in parallel with magnetic valve V6-1, magnetic valve V6-2, magnetic valve V6-3, magnetic valve V6-4.
26 two-position two-way solenoid valves are normally off, DN0.8mm, and inlet and outlet pressure 6bar, KV value is
0.015M3/H, direct current 24VDC, power 3w.
The valve plate base uses PEEK materials, and long side valve plate is provided with fabrication hole, ceramic material is provided with fabrication hole
Spherical plug.
A kind of application method for the post position valve that instrument is isolated and purified for continuous chromatography, it is characterised in that operating procedure is as follows：
(1) when opening magnetic valve V1-5, magnetic valve V2-4, magnetic valve V3-1, magnetic valve V4-1, magnetic valve V5-4 and magnetic valve
V6-2, realize the sample introduction of chromatographic column 1,2,3, the function that chromatographic column 4 elutes；
(1-1) sampling pump, magnetic valve V1-5, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-1, the head of chromatographic column 2, layer
Analyse the afterbody of post 2, magnetic valve V4-1, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-4, waste collection end；
(1-2) elution pump, magnetic valve V2-4, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-2, product collecting terminal；
(2) when opening magnetic valve V1-4, magnetic valve V2-1, magnetic valve V3-3, magnetic valve V4-1, magnetic valve V5-4 and magnetic valve
V6-2, realize chromatographic column 2,3 sample introductions, the function that chromatographic column 1,4 elutes；
(2-1) sampling pump, magnetic valve V1-4, the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-1, the head of chromatographic column 3, layer
Analyse the afterbody of post 3, magnetic valve V5-4, waste collection end；
(2-2) elution pump, magnetic valve V2-1, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-3, the head of chromatographic column 4, layer
Analyse the afterbody of post 4, magnetic valve V6-2, product collecting terminal；
(3) when opening magnetic valve V1-4, magnetic valve V2-1, magnetic valve V3-2, magnetic valve V4-1, magnetic valve V5-1 and magnetic valve
V6-4, realize the sample introduction of chromatographic column 2,3,4, the function that chromatographic column 1 elutes；
(3-1) sampling pump, magnetic valve V1-4, the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-1, the head of chromatographic column 3, layer
Analyse the afterbody of post 3, magnetic valve V5-1, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-4, waste collection end；
(3-2) elution pump, magnetic valve V2-1, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-2, product collecting terminal；
(4) when opening magnetic valve V1-3, magnetic valve V2-2, magnetic valve V3-2, magnetic valve V4-3, magnetic valve V5-1 and magnetic valve
V6-4, realize chromatographic column 3,4 sample introductions, the function that chromatographic column 1,2 elutes；
(4-1) sampling pump, magnetic valve V1-3, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-1, the head of chromatographic column 4, layer
Analyse the afterbody of post 4, magnetic valve V6-4, waste collection end；
(4-2) elution pump, magnetic valve V2-2, the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-3, the head of chromatographic column 1, layer
Analyse the afterbody of post 1, magnetic valve V3-2, product collecting terminal；
(5) when opening magnetic valve V1-3, magnetic valve V2-2, magnetic valve V3-4, magnetic valve V4-2, magnetic valve V5-1 and magnetic valve
V6-1, realize the sample introduction of chromatographic column 1,3,4, the function that chromatographic column 2 elutes；
(5-1) sampling pump, magnetic valve V1-3, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-1, the head of chromatographic column 4, layer
Analyse the afterbody of post 4, magnetic valve V6-1, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-4, waste collection end；
(5-2) elution pump, magnetic valve V2-2, the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-2, product collecting terminal；
(6) when opening magnetic valve V1-2, magnetic valve V2-3, magnetic valve V3-4, magnetic valve V4-2, magnetic valve V5-3 and magnetic valve
V6-1, realize chromatographic column 1,4 sample introductions, the function that chromatographic column 2,3 elutes；
(6-1) sampling pump, magnetic valve V1-2, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-1, the head of chromatographic column 1, layer
Analyse the afterbody of post 1, magnetic valve V3-4, waste collection end；
(6-2) elution pump, magnetic valve V2-3, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-3, the head of chromatographic column 2, layer
Analyse the afterbody of post 2, magnetic valve V4-2, product collecting terminal；
(7) when opening magnetic valve V1-2, magnetic valve V2-3, magnetic valve V3-1, magnetic valve V4-4, magnetic valve V5-2 and magnetic valve
V6-1, realize the sample introduction of chromatographic column 1,2,4, the function that chromatographic column 3 elutes；
(7-1) sampling pump, magnetic valve V1-2, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-1, the head of chromatographic column 1, layer
Analyse the afterbody of post 1, magnetic valve V3-1, the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-4, waste collection end；
(7-2) elution pump, magnetic valve V2-3, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-2, product collecting terminal；
(8) when opening magnetic valve V1-5, magnetic valve V2-4, magnetic valve V3-1, magnetic valve V4-4, magnetic valve V5-2 and magnetic valve
V6-3, realize chromatographic column 1,2 sample introductions, the function that chromatographic column 3,4 elutes；
(8-1) sampling pump, magnetic valve V1-5, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-1, the head of chromatographic column 2, layer
Analyse the afterbody of post 2, magnetic valve V4-4, waste collection end；
(8-2) elution pump, magnetic valve V2-4, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-3, the head of chromatographic column 3, layer
Analyse the afterbody of post 3, magnetic valve 5-2, product collecting terminal；
(9) magnetic valve V1-1 is only opened, realizes the function of sampling pump emptying；
(10) magnetic valve V2-5 is only opened, realizes the function of elution pump emptying；
(11) above-mentioned steps (1) are returned, are repeated.
Usefulness of the present invention：It is proposed by the present invention a kind of to isolate and purify the post position valve of instrument for continuous chromatography and its make
With method, structure novelty is compact, and Method And Principle is clear, and using 4 column systems and valve board structure, flow pipe is set inside valve plate,
Loading does not stop, and saves the batch processed time, and filler utilization rate is high, effectively sample can be avoided to pass, and saves packing volume, saves
Batch processed time and shortening production time play advantageous effect.
Brief description of the drawings
Fig. 1 is post position valve overall structure diagram of the present invention.
Fig. 2 is post position valve overlooking the structure diagram of the present invention.
Fig. 3 is left short side valve plate board face structure schematic diagram in the valve of post position of the present invention.
Fig. 4 is right short side valve plate board face structure schematic diagram in the valve of post position of the present invention.
Fig. 5 is post position of the present invention valve schematic layout pattern in instrument stream is purified.
Fig. 6 is valve internal flow path schematic diagram in post position of the present invention.
Fig. 7 is valve first state stream sketch in post position of the present invention.
Fig. 8 is post position of the present invention valve the second state stream sketch.
Fig. 9 is valve third state stream sketch in post position of the present invention.
Figure 10 is the state stream sketch of post position of the present invention valve the 4th.
Figure 11 is the state stream sketch of post position of the present invention valve the 5th.
Figure 12 is the state stream sketch of post position of the present invention valve the 6th.
Figure 13 is the state stream sketch of post position of the present invention valve the 7th.
Figure 14 is valve eight-shaped state stream sketch in post position of the present invention.
Figure 15 is each electromagnetic valve work state diagram in post post position valve of the present invention.
In figure：1 valve plate pedestal, the long side valve plates of 1-1, the left short side valve plates of 1-2, the tail hole of 1-2-1 posts 1, the head of 1-2-2 posts 4
Hole, the head bore of 1-2-3 posts 2,1-2-4 products collecting terminal, the head bore of 1-2-5 posts 1,1-2-6 elution pumps connecting hole, the tail of 1-2-7 posts 2
Portion hole, the head bore of 1-2-8 posts 3,1-3 tops valve plate, the right short side valve plates of 1-4, the tail hole of 1-4-1 posts 4, the connection of 1-4-2 sampling pumps
Hole, 1-4-3 waste collections end, the tail hole of 1-4-4 posts 3,2 front-seat solenoid valve blocks, 21 magnetic valve V5-1,22 magnetic valve V5-2,23
Magnetic valve V5-3,24 magnetic valve V5-4,25 magnetic valve V3-1,26 magnetic valve V3-2,27 magnetic valve V3-3,28 magnetic valve V3-4,
31 magnetic valve V1-1,32 magnetic valve V1-2,33 magnetic valve V1-3,34 magnetic valve V1-4,35 magnetic valve V1-5,36 magnetic valve V2-
1st, 37 magnetic valve V2-2,38 magnetic valve V2-3,39 magnetic valve V2-4,40 magnetic valve V2-5,41 magnetic valve V6-1,42 magnetic valves
V6-2,43 magnetic valve V6-3,44 magnetic valve V6-4,45 magnetic valve V4-1,46 magnetic valve V4-2,47 magnetic valve V4-3,48 electromagnetism
Valve V4-4, middle solenoid valve block 3, heel row solenoid valve block 4, link electrode 5.
Embodiment
The invention will be further described below in conjunction with the accompanying drawings：
As Figure 1-4, a kind of post position valve that instrument is isolated and purified for continuous chromatography, including valve plate base 1, and it is arranged on valve
Sampling pump, elution pump, chromatographic column 1, chromatographic column 2, chromatographic column 3 and the chromatographic column 4 in the outside of plate base 1；The crown valve of valve plate base 1
Plate 1-3 is provided with 26 structure identical two-position two-way solenoid valves, 26 two-position two-way solenoid valves form front-seat solenoid valve block 2,
Middle solenoid valve block 3 and heel row solenoid valve block 4；Front-seat solenoid valve block 2 by magnetic valve V5-1, magnetic valve V5-2, magnetic valve V5-3,
Magnetic valve V5-4, magnetic valve V3-1, magnetic valve V3-2, magnetic valve V3-3 and magnetic valve V3-4 are formed；Middle solenoid valve block is by electricity
Magnet valve V1-1, magnetic valve V1-2, magnetic valve V1-3, magnetic valve V1-4, magnetic valve V1-5, magnetic valve V2-1, magnetic valve V2-2, electricity
Magnet valve V2-3, magnetic valve V2-4 and magnetic valve V2-5 are formed；Heel row solenoid valve block is by magnetic valve V6-1, magnetic valve V6-2, electromagnetism
Valve V6-3, magnetic valve V6-4, magnetic valve V4-1, magnetic valve V4-2, magnetic valve V4-3 and magnetic valve V4-4 are formed；26 two two
Three-way electromagnetic valve is normally off, and DN0.8mm, inlet and outlet pressure 6bar, KV value are 0.015M3/H, direct current 24VDC, power 3w.Valve
Plate base 1 uses PEEK materials, and long side valve plate 1-1 is provided with fabrication hole, and the spherical plug of ceramic material is provided with fabrication hole.
As Figure 1-4, a kind of post position valve that instrument is isolated and purified for continuous chromatography, the inside of valve plate pedestal 1 are provided with post position
The connection stream of valve, left short side valve plate 1-2 be provided with the head bore 1-2-5 of post 1, the tail hole 1-2-1 of post 1, the head bore 1-2-3 of post 2,
The tail hole 1-2-7 of post 2, the head bore 1-2-8 of post 3, the head bore 1-2-2 of post 4, elution pump connecting hole 1-2-6 and product collecting terminal 1-
2-4；Right short side valve plate 1-4 is provided with the tail hole 1-4-1 of post 4, sampling pump connecting hole 1-4-2, waste collection end 1-4-3 and post 3
Tail hole 1-4-4；The head bore 1-2-5 of the post 1 and tail hole 1-2-1 of post 1 and the headtotail of chromatographic column 1, the head bore 1-2-3 of post 2 and
The tail hole 1-2-7 of post 2 and the headtotail of chromatographic column 2, the head bore 1-2-8 of post 3 and the tail hole 1-4-4 of post 3 and chromatographic column 3 phase end to end
Connect, the head bore 1-2-2 of post 4 and the tail hole 1-4-1 of post 4 and the headtotail of chromatographic column 4, elution pump connecting hole 1-2-6 and elution pump
Connect, sampling pump connecting hole 1-4-2 connects with sampling pump；
As Figure 1-4, a kind of post position valve that instrument is isolated and purified for continuous chromatography, the connection stream composition of post position valve are as follows：
(1) sampling pump connecting hole 1-4-2 and magnetic valve V1-1, magnetic valve V1-2, magnetic valve V1-3, magnetic valve V1-4, magnetic valve
V1-5 is formed and is connected in parallel；Wherein, magnetic valve V1-1 is connected with waste collection end 1-4-3；Magnetic valve V1-2 and magnetic valve V5-1,
Product collecting terminal 1-2-4 is connected to after magnetic valve V5-2 series connection；After magnetic valve V1-3 connects with magnetic valve V4-1, magnetic valve V4-2
It is connected to product collecting terminal 1-2-4；Magnetic valve V1-4 is connected to product collecting terminal after being connected with magnetic valve V3-1, magnetic valve V3-2
1-2-4；Magnetic valve V1-5 is connected to product collecting terminal 1-2-4 after being connected with magnetic valve V6-1, magnetic valve V6-2；
(2) pump connecting hole 1-2-6 and magnetic valve V2-1, magnetic valve V2-2, magnetic valve V2-3, magnetic valve V2-4, magnetic valve are eluted
V2-5 is formed and is connected in parallel；Wherein, magnetic valve V2-1 is connected to waste collection end after being connected with magnetic valve V4-3, magnetic valve V4-4
1-4-3；Magnetic valve V2-2 is connected to waste collection end 1-4-3 after being connected with magnetic valve V5-3, magnetic valve V5-4；Magnetic valve V2-3
Waste collection end 1-4-3 is connected to after being connected with magnetic valve V6-3, magnetic valve V6-4；Magnetic valve V2-4 and magnetic valve V3-3, electricity
Waste collection end 1-4-3 is connected to after magnet valve V3-4 series connection；Magnetic valve V2-5 is connected to product collecting terminal 1-2-4；
(3) the head bore 1-2-5 of post 1 and magnetic valve V1-5, magnetic valve the V2-1 port of export are connected in parallel；The head bore 1-2-3 of post 2 with
Magnetic valve V1-4, the magnetic valve V2-2 port of export are connected in parallel；The head bore 1-2-8 of post 3 and magnetic valve V1-3, magnetic valve V2-3
The port of export is connected in parallel；The head bore 1-2-2 of post 4 and magnetic valve V1-2, magnetic valve the V2-4 port of export are connected in parallel；
(4) the tail hole 1-2-1 of post 1 forms phase in parallel with magnetic valve V3-1, magnetic valve V3-2, magnetic valve V3-3, magnetic valve V3-4
Connect；The tail hole 1-2-7 of post 2 and magnetic valve V4-1, magnetic valve V4-2, magnetic valve V4-3, magnetic valve V4-4 are formed and are connected in parallel；Post
3 tail hole 1-4-4 and magnetic valve V5-1, magnetic valve V5-2, magnetic valve V5-3, magnetic valve V5-4 are formed and are connected in parallel；The afterbody of post 4
Hole 1-4-1 and magnetic valve V6-1, magnetic valve V6-2, magnetic valve V6-3, magnetic valve V6-4 are formed and are connected in parallel.
A kind of application method for the post position valve that instrument is isolated and purified for continuous chromatography, operating procedure are as follows：
As shown in Fig. 5,7,15, magnetic valve V1-5, magnetic valve V2-4, magnetic valve V3-1, magnetic valve V4-1, magnetic valve V5-4 are opened
With magnetic valve V6-2, the sample introduction of chromatographic column 1,2,3, the function that chromatographic column 4 elutes are realized；
Sampling pump, magnetic valve V1-5, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-1, the head of chromatographic column 2, chromatographic column 2
Afterbody, magnetic valve V4-1, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-4, waste collection end 1-4-3；Elute pump, electricity
Magnet valve V2-4, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,8,15, magnetic valve V1-4, magnetic valve V2-1, magnetic valve V3-3, magnetic valve V4-1, magnetic valve are opened
V5-4 and magnetic valve V6-2, realize chromatographic column 2,3 sample introductions, the function that chromatographic column 1,4 elutes；Sampling pump, magnetic valve V1-4, chromatography
The head of post 2, the afterbody of chromatographic column 2, magnetic valve V4-1, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-4, waste collection end
1-4-3；Elute pump, magnetic valve V2-1, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-3, the head of chromatographic column 4, chromatographic column
4 afterbodys, magnetic valve V6-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,9,15, magnetic valve V1-4, magnetic valve V2-1, magnetic valve V3-2, magnetic valve V4-1, magnetic valve are opened
V5-1 and magnetic valve V6-4, realize the sample introduction of chromatographic column 2,3,4, the function that chromatographic column 1 elutes；Sampling pump, magnetic valve V1-4, chromatography
The head of post 2, the afterbody of chromatographic column 2, magnetic valve V4-1, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-1, chromatographic column 4
Portion, the afterbody of chromatographic column 4, magnetic valve V6-4, waste collection end 1-4-3；Elute pump, magnetic valve V2-1, the head of chromatographic column 1, chromatography
The afterbody of post 1, magnetic valve V3-2, product collecting terminal；
As shown in Fig. 5,6,10,15, magnetic valve V1-3, magnetic valve V2-2, magnetic valve V3-2, magnetic valve V4-3, magnetic valve are opened
V5-1 and magnetic valve V6-4, realize chromatographic column 3,4 sample introductions, the function that chromatographic column 1,2 elutes；Sampling pump, magnetic valve V1-3, chromatography
The head of post 3, the afterbody of chromatographic column 3, magnetic valve V5-1, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-4, waste collection end
1-4-3；Elute pump, magnetic valve V2-2, the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-3, the head of chromatographic column 1, chromatographic column
1 afterbody, magnetic valve V3-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,11,15, magnetic valve V1-3, magnetic valve V2-2, magnetic valve V3-4, magnetic valve V4-2, magnetic valve are opened
V5-1 and magnetic valve V6-1, realize the sample introduction of chromatographic column 1,3,4, the function that chromatographic column 2 elutes；Sampling pump, magnetic valve V1-3, chromatography
The head of post 3, the afterbody of chromatographic column 3, magnetic valve V5-1, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-1, chromatographic column 1
Portion, the afterbody of chromatographic column 1, magnetic valve V3-4, waste collection end 1-4-3；Elute pump, magnetic valve V2-2, the head of chromatographic column 2, chromatography
The afterbody of post 2, magnetic valve V4-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,12,15, magnetic valve V1-2, magnetic valve V2-3, magnetic valve V3-4, magnetic valve V4-2, magnetic valve are opened
V5-3 and magnetic valve V6-1, realize chromatographic column 1,4 sample introductions, the function that chromatographic column 2,3 elutes；Sampling pump, magnetic valve V1-2, chromatography
The head of post 4, the afterbody of chromatographic column 4, magnetic valve V6-1, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-4, waste collection end
1-4-3；Elute pump, magnetic valve V2-3, the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-3, the head of chromatographic column 2, chromatographic column
2 afterbodys, magnetic valve V4-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,13,15, magnetic valve V1-2, magnetic valve V2-3, magnetic valve V3-1, magnetic valve V4-4, magnetic valve are opened
V5-2 and magnetic valve V6-1, realize the sample introduction of chromatographic column 1,2,4, the function that chromatographic column 3 elutes；Sampling pump, magnetic valve V1-2, chromatography
The head of post 4, the afterbody of chromatographic column 4, magnetic valve V6-1, the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-1, chromatographic column 2
Portion, the afterbody of chromatographic column 2, magnetic valve V4-4, waste collection end 1-4-3；Elute pump, magnetic valve V2-3, the head of chromatographic column 3, chromatography
The afterbody of post 3, magnetic valve V5-2, product collecting terminal 1-2-4；
As shown in Fig. 5,6,14,15, magnetic valve V1-5, magnetic valve V2-4, magnetic valve V3-1, magnetic valve V4-4, magnetic valve are opened
V5-2 and magnetic valve V6-3, realize chromatographic column 1,2 sample introductions, the function that chromatographic column 3,4 elutes；Sampling pump, magnetic valve V1-5, chromatography
The head of post 1, the afterbody of chromatographic column 1, magnetic valve V3-1, the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-4, waste collection end
1-4-3；Elute pump, magnetic valve V2-4, the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-3, the head of chromatographic column 3, chromatographic column
3 afterbodys, magnetic valve 5-2, product collecting terminal 1-2-4；
Such as the opening magnetic valve V1-1 of Fig. 5,6,15, the function that sampling pump empties is realized；Magnetic valve V2-5 is opened, realizes elution pump row
Empty function.
Claims (4)
1. a kind of post position valve that instrument is isolated and purified for continuous chromatography, including valve plate base (1), are arranged on valve plate base (1) outside
Sampling pump, elution pump, chromatographic column 1, chromatographic column 2, chromatographic column 3 and the chromatographic column 4 of side；It is characterized in that：The valve plate base (1)
Top valve plate (1-3) be provided with 26 structure identical two-position two-way solenoid valves, 26 two-position two-way solenoid valves are formed
Front-seat solenoid valve block (2), middle solenoid valve block (3) and heel row solenoid valve block (4)；
The front-seat solenoid valve block (2) is by magnetic valve V5-1 (21), magnetic valve V5-2 (22), magnetic valve V5-3 (23), magnetic valve
V5-4 (24), magnetic valve V3-1 (25), magnetic valve V3-2 (26), magnetic valve V3-3 (27) and magnetic valve V3-4 (28) are formed；
The middle solenoid valve block is by magnetic valve V1-1 (31), magnetic valve V1-2 (32), magnetic valve V1-3 (33), magnetic valve V1-4
(34), magnetic valve V1-5 (35), magnetic valve V2-1 (36), magnetic valve V2-2 (37), magnetic valve V2-3 (38), magnetic valve V2-4
(39) formed with magnetic valve V2-5 (40)；
The heel row solenoid valve block is by magnetic valve V6-1 (41), magnetic valve V6-2 (42), magnetic valve V6-3 (43), magnetic valve V6-4
(44), magnetic valve V4-1 (45), magnetic valve V4-2 (46), magnetic valve V4-3 (47) and magnetic valve V4-4 (48) are formed；
The connection stream of post position valve is provided with inside the valve plate pedestal (1), left short side valve plate (1-2) is provided with the head bore (1- of post 1
2-5), the tail hole of post 1 (1-2-1), the head bore of post 2 (1-2-3), the tail hole of post 2 (1-2-7), the head bore of post 3 (1-2-8), post 4
Head bore (1-2-2), elution pump connecting hole (1-2-6) and product collecting terminal (1-2-4)；Right short side valve plate (1-4) is provided with post 4
Tail hole (1-4-1), sampling pump connecting hole (1-4-2), waste collection end (1-4-3) and the tail hole of post 3 (1-4-4)；The post 1
Head bore (1-2-5) and the tail hole of post 1 (1-2-1) and the headtotail of chromatographic column 1, the head bore of post 2 (1-2-3) and post
2 tail holes (1-2-7) and the headtotail of chromatographic column 2, the head bore of post 3 (1-2-8) and the tail hole of post 3 (1-4-4) and
The headtotail of chromatographic column 3, the head bore of post 4 (1-2-2) and the tail hole of post 4 (1-4-1) and the chromatographic column 4 phase end to end
Connect, the elution pump connecting hole (1-2-6) connects with the elution pump, the sampling pump connecting hole (1-4-2) and the sample introduction
Pump connects；
The connection stream of post position valve forms as follows：
(1) the sampling pump connecting hole (1-4-2) and the magnetic valve V1-1 (31), magnetic valve V1-2 (32), magnetic valve V1-3
(33), magnetic valve V1-4 (34), magnetic valve V1-5 (35) are formed and are connected in parallel；Wherein, the magnetic valve V1-1 (31) with it is described
Waste collection end (1-4-3) is connected；After the magnetic valve V1-2 (32) is connected with magnetic valve V5-1 (21), magnetic valve V5-2 (22)
It is connected to the product collecting terminal (1-2-4)；The magnetic valve V1-3 (33) and magnetic valve V4-1 (45), magnetic valve V4-2 (46)
The product collecting terminal (1-2-4) is connected to after series connection；The magnetic valve V1-4 (34) and magnetic valve V3-1 (25), magnetic valve
The product collecting terminal (1-2-4) is connected to after V3-2 (26) series connection；The magnetic valve V1-5 (35) and magnetic valve V6-1 (41),
The product collecting terminal (1-2-4) is connected to after magnetic valve V6-2 (42) series connection；
(2) the elution pump connecting hole (1-2-6) and the magnetic valve V2-1 (36), magnetic valve V2-2 (37), magnetic valve V2-3
(38), magnetic valve V2-4 (39), magnetic valve V2-5 (40) are formed and are connected in parallel；Wherein, the magnetic valve V2-1 (36) and electromagnetism
The waste collection end (1-4-3) is connected to after valve V4-3 (47), magnetic valve V4-4 (48) series connection；The magnetic valve V2-2 (37)
The waste collection end (1-4-3) is connected to after being connected with magnetic valve V5-3 (23), magnetic valve V5-4 (24)；The magnetic valve
V2-3 (38) is connected to the waste collection end (1-4-3) after being connected with magnetic valve V6-3 (43), magnetic valve V6-4 (44)；It is described
Magnetic valve V2-4 (39) is connected to the waste collection end (1-4- after being connected with magnetic valve V3-3 (27), magnetic valve V3-4 (28)
3)；The magnetic valve V2-5 (40) is connected to the product collecting terminal (1-2-4)；
(3) head bore of post 1 (1-2-5) and magnetic valve V1-5 (35), magnetic valve V2-1 (36) port of export are connected in parallel；Institute
State the head bore of post 2 (1-2-3) and magnetic valve V1-4 (34), magnetic valve V2-2 (37) port of export are connected in parallel；The head of post 3
Hole (1-2-8) and magnetic valve V1-3 (33), magnetic valve V2-3 (38) port of export are connected in parallel；The head bore of post 4 (1-2-2)
It is connected in parallel with magnetic valve V1-2 (32), magnetic valve V2-4 (39) port of export；
(4) tail hole of post 1 (1-2-1) and magnetic valve V3-1 (25), magnetic valve V3-2 (26), magnetic valve V3-3 (27), electricity
Magnet valve V3-4 (28) is formed and is connected in parallel；The tail hole of post 2 (1-2-7) and magnetic valve V4-1 (45), magnetic valve V4-2 (46),
Magnetic valve V4-3 (47), magnetic valve V4-4 (48) are formed and are connected in parallel；The tail hole of post 3 (1-4-4) and magnetic valve V5-1
(21), magnetic valve V5-2 (22), magnetic valve V5-3 (23), magnetic valve V5-4 (24) are formed and are connected in parallel；The tail hole of post 4
(1-4-1) is in parallel with magnetic valve V6-1 (41), magnetic valve V6-2 (42), magnetic valve V6-3 (43), magnetic valve V6-4 (44) formation
Connect.
A kind of 2. post position valve that instrument is isolated and purified for continuous chromatography according to claim 1, it is characterised in that：Described 26
Two-position two-way solenoid valve is normally off, DN0.8mm, and inlet and outlet pressure 6bar, KV value are 0.015M3/H, direct current 24VDC,
Power 3w.
A kind of 3. post position valve that instrument is isolated and purified for continuous chromatography according to claim 1, it is characterised in that：The valve
Plate base (1) uses PEEK materials, and long side valve plate (1-1) is provided with fabrication hole, and the spherical stifled of ceramic material is provided with fabrication hole
Head.
4. a kind of application method of post position valve that instrument is isolated and purified for continuous chromatography as claimed in claim 1, its feature exist
In operating procedure is as follows：
(1) magnetic valve V1-5 (35), magnetic valve V2-4 (39), magnetic valve V3-1 (25), magnetic valve V4-1 (45), magnetic valve are opened
V5-4 (24) and magnetic valve V6-2 (42), realize the sample introduction of chromatographic column 1,2,3, the function that chromatographic column 4 elutes；
(1-1) sampling pump, magnetic valve V1-5 (35), the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-1 (25), chromatographic column 2
Head, the afterbody of chromatographic column 2, magnetic valve V4-1 (45), the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-4 (24), waste liquid are received
Collect end (1-4-3)；
(1-2) elution pump, magnetic valve V2-4 (39), the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-2 (42), product are received
Collect end (1-2-4)；
(2) magnetic valve V1-4 (34), magnetic valve V2-1 (36), magnetic valve V3-3 (27), magnetic valve V4-1 (45), magnetic valve are opened
V5-4 (24) and magnetic valve V6-2 (42), realize chromatographic column 2,3 sample introductions, the function that chromatographic column 1,4 elutes；
(2-1) sampling pump, magnetic valve V1-4 (34), the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-1 (45), chromatographic column 3
Head, the afterbody of chromatographic column 3, magnetic valve V5-4 (24), waste collection end (1-4-3)；
(2-2) elution pump, magnetic valve V2-1 (36), the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-3 (27), chromatographic column 4
Head, the afterbody of chromatographic column 4, magnetic valve V6-2 (42), product collecting terminal (1-2-4)；
(3) magnetic valve V1-4 (34), magnetic valve V2-1 (36), magnetic valve V3-2 (26), magnetic valve V4-1 (45), magnetic valve are opened
V5-1 (21) and magnetic valve V6-4 (44), realize the sample introduction of chromatographic column 2,3,4, the function that chromatographic column 1 elutes；
(3-1) sampling pump, magnetic valve V1-4 (34), the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-1 (45), chromatographic column 3
Head, the afterbody of chromatographic column 3, magnetic valve V5-1 (21), the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-4 (44), waste liquid are received
Collect end (1-4-3)；
(3-2) elution pump, magnetic valve V2-1 (36), the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-2 (26), product are received
Collect end (1-2-4)；
(4) magnetic valve V1-3 (33), magnetic valve V2-2 (37), magnetic valve V3-2 (26), magnetic valve V4-3 (47), magnetic valve are opened
V5-1 (21) and magnetic valve V6-4 (44), realize chromatographic column 3,4 sample introductions, the function that chromatographic column 1,2 elutes；
(4-1) sampling pump, magnetic valve V1-3 (33), the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-1 (21), chromatographic column 4
Head, the afterbody of chromatographic column 4, magnetic valve V6-4 (44), waste collection end (1-4-3)；
(4-2) elution pump, magnetic valve V2-2 (37), the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-3 (47), chromatographic column 1
Head, the afterbody of chromatographic column 1, magnetic valve V3-2 (26), product collecting terminal (1-2-4)；
(5) magnetic valve V1-3 (33), magnetic valve V2-2 (37), magnetic valve V3-4 (28), magnetic valve V4-2 (46), magnetic valve are opened
V5-1 (21) and magnetic valve V6-1 (41), realize the sample introduction of chromatographic column 1,3,4, the function that chromatographic column 2 elutes；
(5-1) sampling pump, magnetic valve V1-3 (33), the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-1 (21), chromatographic column 4
Head, the afterbody of chromatographic column 4, magnetic valve V6-1 (41), the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-4 (28), waste liquid are received
Collect end (1-4-3)；
(5-2) elution pump, magnetic valve V2-2 (37), the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-2 (46), product are received
Collect end (1-2-4)；
(6) magnetic valve V1-2 (32), magnetic valve V2-3 (38), magnetic valve V3-4 (28), magnetic valve V4-2 (46), magnetic valve are opened
V5-3 (23) and magnetic valve V6-1 (41), realize chromatographic column 1,4 sample introductions, the function that chromatographic column 2,3 elutes；
(6-1) sampling pump, magnetic valve V1-2 (32), the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-1 (41), chromatographic column 1
Head, the afterbody of chromatographic column 1, magnetic valve V3-4 (28), waste collection end (1-4-3)；
(6-2) elution pump, magnetic valve V2-3 (38), the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-3 (23), chromatographic column 2
Head, the afterbody of chromatographic column 2, magnetic valve V4-2 (46), product collecting terminal (1-2-4)；
(7) magnetic valve V1-2 (32), magnetic valve V2-3 (38), magnetic valve V3-1 (25), magnetic valve V4-4 (48), magnetic valve are opened
V5-2 (22) and magnetic valve V6-1 (41), realize the sample introduction of chromatographic column 1,2,4, the function that chromatographic column 3 elutes；
(7-1) sampling pump, magnetic valve V1-2 (32), the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-1 (41), chromatographic column 1
Head, the afterbody of chromatographic column 1, magnetic valve V3-1 (25), the head of chromatographic column 2, the afterbody of chromatographic column 2, magnetic valve V4-4 (48), waste liquid are received
Collect end (1-4-3)；
(7-2) elution pump, magnetic valve V2-3 (38), the head of chromatographic column 3, the afterbody of chromatographic column 3, magnetic valve V5-2 (22), product are received
Collect end (1-2-4)；
(8) magnetic valve V1-5 (35), magnetic valve V2-4 (39), magnetic valve V3-1 (25), magnetic valve V4-4 (48), magnetic valve are opened
V5-2 (22) and magnetic valve V6-3 (43), realize chromatographic column 1,2 sample introductions, the function that chromatographic column 3,4 elutes；
(8-1) sampling pump, magnetic valve V1-5 (35), the head of chromatographic column 1, the afterbody of chromatographic column 1, magnetic valve V3-1 (25), chromatographic column 2
Head, the afterbody of chromatographic column 2, magnetic valve V4-4 (48), waste collection end (1-4-3)；
(8-2) elution pump, magnetic valve V2-4 (39), the head of chromatographic column 4, the afterbody of chromatographic column 4, magnetic valve V6-3 (43), chromatographic column 3
Head, the afterbody of chromatographic column 3, magnetic valve 5-2 (22), product collecting terminal (1-2-4)；
(9) magnetic valve V1-1 (31) is opened, realizes the function of sampling pump emptying；
(10) magnetic valve V2-5 (40) is opened, realizes the function of elution pump emptying；
(11) above-mentioned steps (1) are returned, are repeated.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710815322.5A CN107376423B (en) | 2017-09-12 | 2017-09-12 | A kind of column position valve and its application method isolating and purifying instrument for continuous chromatography |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710815322.5A CN107376423B (en) | 2017-09-12 | 2017-09-12 | A kind of column position valve and its application method isolating and purifying instrument for continuous chromatography |
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| Publication Number | Publication Date |
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| CN107376423A true CN107376423A (en) | 2017-11-24 |
| CN107376423B CN107376423B (en) | 2019-09-10 |
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| CN110327660A (en) * | 2019-07-23 | 2019-10-15 | 汕头大学 | A kind of dip-adjustable type counter-current chromatograph drive mechanism |
| CN110327660B (en) * | 2019-07-23 | 2021-07-13 | 汕头大学 | Transmission structure of counter-current chromatograph with adjustable inclination angle |
| CN116672760A (en) * | 2023-08-03 | 2023-09-01 | 成都百泉生物医药科技有限公司 | Solid phase extraction instrument |
| CN116672760B (en) * | 2023-08-03 | 2023-10-20 | 成都百泉生物医药科技有限公司 | Solid phase extraction instrument |
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| CN107376423B (en) | 2019-09-10 |
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