CN202527079U - Fetal bovine blood removing bacteria and mycoplasma removing assorted device - Google Patents
Fetal bovine blood removing bacteria and mycoplasma removing assorted device Download PDFInfo
- Publication number
- CN202527079U CN202527079U CN201220014174XU CN201220014174U CN202527079U CN 202527079 U CN202527079 U CN 202527079U CN 201220014174X U CN201220014174X U CN 201220014174XU CN 201220014174 U CN201220014174 U CN 201220014174U CN 202527079 U CN202527079 U CN 202527079U
- Authority
- CN
- China
- Prior art keywords
- liquid outlet
- reservoir
- liquid inlet
- poly
- mycoplasma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model discloses a fetal bovine blood removing bacteria and mycoplasma removing assorted device, which comprises a reservoir, a deep layer filter system, an ultrafiltration system, a polyether sulfone membrane filter system and a collecting system, wherein the liquid outlet of the reservoir is connected with the liquid inlet of the deep layer filter system; the liquid outlet of the deep layer filter system is connected with the liquid inlet of the ultrafiltration system; the liquid outlet of the ultrafiltration system is connected with the liquid inlet of the polyether sulfone membrane filter system; and the liquid outlet of the polyether sulfone membrane filter system is connected with the liquid inlet of the collecting system. The device is reasonable for manufacturing and installing, is convenient to operate, and is reliable for using.
Description
Technical field
The utility model device relates to molecular biology; Utilize filter membrane and milipore filter that hyclone is carried out degerming, removes mycoplasma; Be specifically related to in-depth filtration and remove some impurity in the hyclone; Tentatively remove material and bacterium, and then hyclone is carried out ultrafiltration, to remove some macromolecular substances and thermal source through milipore filter greater than 0.2 micron; Last poly (ether sulfone) film through 0.1 micron more finally removes and degerms and microorganism such as mycoplasma, finally contacting the film system of three cover filtrations, ultrafiltration with silica gel hose.
Background technology
In the present domestic cow's serum production technology; All be to adopt the method for filtering, can only simply reach degermation, the pair cell culture requirement also is nowhere near; Domestic majority cell majority has all polluted mycoplasma etc.; And the endotoxin content of product all can exceed standard, and batch homogeneity of serum is very unstable, so the import serum product can be selected mostly by most of biological products producer and research institutions.
Summary of the invention
The degerming of the utility model hyclone, remove mycoplasma, purifying complexes application aims is to overcome above-mentioned deficiency; And a kind of hyclone degerming is provided, removes the mycoplasma device; And remove some pair cells and cultivate useless macromolecular substances, make the hyclone product have that nutrient content is stable, characteristics such as differences between batches are little, endotoxin is low, aseptic no mycoplasma.Concrete technical scheme is following:
A kind of degerming, remove mycoplasma, the device of purifying hyclone comprises reservoir, depth filtration systems, ultrafiltration system, poly (ether sulfone) film filtration system and gathering system; The liquid outlet of said reservoir connects the liquid inlet of depth filtration systems; The liquid outlet of depth filtration systems connects the liquid inlet of ultrafiltration system; Ultrafiltration system liquid outlet connect the liquid inlet of poly (ether sulfone) film filtration system, the liquid outlet of poly (ether sulfone) film filtration system connects the liquid inlet of gathering system.
Said depth filtration systems is the deep layer film filter; The deep layer film filter comprises one group of 0.65 micron polypropylene screen filter and one group of 0.2 micron polypropylene screen filter; One group 0.65 micron polypropylene screen filter liquid outlet of deep layer film filter connects the liquid inlet of 0.2 micron polypropylene screen filter.Depth filtration systems is mainly removed some impurity in the hyclone and greater than 0.2 micron material and bacterium.
Said ultrafiltration system is 10000 daltonian poly (ether sulfone) films.To remove some macromolecular substances and thermal source, these macromolecular substances pair cells are cultivated all useless, have reached the purpose of purifying.
Said poly (ether sulfone) film filtration system is the poly (ether sulfone) film filter, and this is that the poly (ether sulfone) film filter is 0.1 micron poly (ether sulfone) film.Final removing degermed and microorganism such as mycoplasma, reached the cell culture requirement.
The liquid outlet of said reservoir is installed in the bottom of reservoir, and liquid outlet is provided with valve; The top of reservoir is equipped with respirator; Be connected with first peristaltic pump between the liquid outlet of reservoir and the liquid inlet of depth filtration systems; Between the liquid outlet of reservoir and first peristaltic pump, and be connected through silica gel hose between the liquid inlet of depth filtration systems and first peristaltic pump.Regulate the speed of peristaltic pump according to actual needs.
Said gathering system is to stir fluid reservoir; The top of stirring fluid reservoir is equipped with liquid inlet and respirator; The bottom of stirring fluid reservoir is equipped with liquid outlet, and valve is installed on the liquid outlet; This liquid outlet is connected with second peristaltic pump, and liquid outlet is connected with the liquid inlet of second peristaltic pump, and the liquid outlet of second peristaltic pump connects the liquid inlet that outer mechanical irrigation installing is equipped with.
The hyclone that comes out through the poly (ether sulfone) film filtration system gets into the fluid reservoir of collecting hyclone, after be distributed into finished product packing and dispatch from the factory.
It is that 0.65 micron, 0.2 micron polypropylene screen can go impurity and bacterium in the hyclone that the advantage of the utility model has been to select the aperture; Milipore filter through 10000 molecular weight carries out purifying again; To remove some macromolecular substances and thermal source; Last again through the degerming of polyether sulfone filter membrane, remove mycoplasma, reached the cultivation requirement that meets multiple enervated cell.The utility model device make reasonable installation, easy to operate, use reliable, with realize simply, rapidly, continuously, the purpose of High-efficient Production.Novel hyclone degerming, remove mycoplasma, the purifying complexes can be removed some pair cells and cultivate useless macromolecular substances, make the hyclone product have that nutrient content is stable, characteristics such as differences between batches are little, endotoxin is low, aseptic no mycoplasma.
Description of drawings
Fig. 1 is a kind of hyclone degerming, remove mycoplasma complexes sketch map;
Among the figure, 1, fluid reservoir (being used for storing raw material), 2, the fluid reservoir respirator, 3, the fluid reservoir hand-operated valve; 4, first peristaltic pump, 5,0.65 microns polypropylene screen filters, 6,0.2 microns polypropylene screen filters, 7, poly (ether sulfone) film ultrafiltration system (10000 daltonian poly (ether sulfone) film); 8,0.1 micron poly (ether sulfone) film (poly (ether sulfone) film filter), 9, stir the fluid reservoir inlet, 10, stirring motor; 11, agitating device, 12 stir fluid reservoir hand-operated valve, 13 second peristaltic pumps; 14 stir fluid reservoirs (being used for storing semi-finished product), and 15, stir fluid reservoir jar respirator, 16, the finished product bottle placer.
The specific embodiment
The combination accompanying drawing further specifies the utility model device below:
A kind of degerming, remove mycoplasma, the device of purifying hyclone comprises reservoir, depth filtration systems, ultrafiltration system, poly (ether sulfone) film filtration system and gathering system; The liquid outlet of said reservoir connects the liquid inlet of depth filtration systems; The liquid outlet of depth filtration systems connects the liquid inlet of ultrafiltration system; Ultrafiltration system liquid outlet connect the liquid inlet of poly (ether sulfone) film filtration system, the liquid outlet of poly (ether sulfone) film filtration system connects the liquid inlet of gathering system.
Said depth filtration systems is the deep layer film filter; The deep layer film filter comprises one group of 0.65 micron polypropylene screen filter and one group of 0.2 micron polypropylene screen filter; One group 0.65 micron polypropylene screen filter liquid outlet of deep layer film filter connects the liquid inlet of 0.2 micron polypropylene screen filter.
Said ultrafiltration system is 10000 daltonian poly (ether sulfone) films.
Said poly (ether sulfone) film filtration system is the poly (ether sulfone) film filter, and this is that the poly (ether sulfone) film filter is 0.1 micron poly (ether sulfone) film.
The liquid outlet of said reservoir is installed in the bottom of reservoir, and liquid outlet is provided with valve; The top of reservoir is equipped with respirator; Be connected with first peristaltic pump between the liquid outlet of reservoir and the liquid inlet of depth filtration systems; Between the liquid outlet of reservoir and first peristaltic pump, and be connected through silica gel hose between the liquid inlet of depth filtration systems and first peristaltic pump.
Said gathering system is to stir fluid reservoir; The top of stirring fluid reservoir is equipped with liquid inlet and respirator; The bottom of stirring fluid reservoir is equipped with liquid outlet, and valve is installed on the liquid outlet; This liquid outlet is connected with second peristaltic pump, and liquid outlet is connected with the liquid inlet of second peristaltic pump, and the liquid outlet of second peristaltic pump connects the liquid inlet that outer mechanical irrigation installing is equipped with.
This device; Comprise three-stage filtration systems such as deep layer membrane filtration system, ultrafiltration system, ultimate filtration system; This three cover system is connected in series through the order of silica gel hose by deep layer membrane filtration system, ultrafiltration system, ultimate filtration system; The front end of three cover trains has the fluid reservoir of hyclone, and the tail end of three cover trains has ultimate finished product fluid reservoir.Between the fluid reservoir of hyclone and the membrane filtration system peristaltic pump is installed, between ultimate finished product fluid reservoir and the finished product can peristaltic pump is installed also, peristaltic pump provides the power of serum liquid flow.All parts link to each other and have all used health level silica gel hose.Be connected with feed pipe, respirator on the said raw material fluid reservoir; The fluid reservoir bottom also has discharging opening, and discharging opening inserts peristaltic pump with flexible pipe, and the port of export of peristaltic pump connects deep layer film filter, peristaltic pump.
The deep layer membrane filtration system is made up of two polypropylene screen filters, and first is 0.65 micron polypropylene screen filter, and second is 0.2 micron polypropylene screen filter.The outlet of first polypropylene deep layer molecular filter is connected with the import of second polypropylene deep layer film filter.The outlet of second film filter of deep layer membrane filtration system is connected with ultrafiltration system.Ultrafiltration system is connected with ultimate filtration system, and ultimate filtration system is 0.1 micron polyether sulfone filter membrane, removes the mycoplasma effect thereby reach degerming.The level polyether sulfone is directly connected to ultimate fluid reservoir eventually, and motor is arranged on the fluid reservoir, drives agitator the hyclone in the fluid reservoir is stirred, thereby make the product uniformity.Fluid reservoir is provided with respirator, and outlet at bottom has valve, opens valve, the packing hyclone.The level fluid reservoir connects peristaltic pump eventually, and peristaltic pump connects the finished product bottle placer.
This device in use; Open the valve 3 in the raw material fluid reservoir 1, drive peristaltic pump 4, the filter in 2 groups of different apertures through the polypropylene filter membrane is housed; And then pass through poly (ether-sulfone) ultrafiltration membrane; At last through the filter of 0.1 micron polyether sulfone filter membrane is housed, hyclone all filter, ultrafiltration goes into fluid reservoir, sell through the peristaltic pump packing more at last.The filter size is relevant with the size of film, and it is fixed that film can come according to output, can be 10 inches, 20 inches, 30 inches, also can divide single core, 3 cores, 5 cores, 7 cores etc., and single batch of output can be accomplished 2,000,000 milliliters of hyclones.
This device make reasonable installation, easy to operate, use reliable.
Claims (6)
1. a hyclone degerming, remove the mycoplasma complexes, comprise reservoir, depth filtration systems, ultrafiltration system, poly (ether sulfone) film filtration system and gathering system; The liquid outlet of said reservoir connects the liquid inlet of depth filtration systems; The liquid outlet of depth filtration systems connects the liquid inlet of ultrafiltration system; The liquid outlet of ultrafiltration system connects the liquid inlet of poly (ether sulfone) film filtration system, and the liquid outlet of poly (ether sulfone) film filtration system connects the liquid inlet of gathering system.
2. hyclone degerming according to claim 1, remove the mycoplasma complexes, it is characterized in that said depth filtration systems is the deep layer film filter; The deep layer film filter comprises one group of 0.65 micron polypropylene screen filter and one group of 0.2 micron polypropylene screen filter; One group 0.65 micron polypropylene screen filter liquid outlet of deep layer film filter connects the liquid inlet of 0.2 micron polypropylene screen filter.
3. hyclone degerming according to claim 1, remove the mycoplasma complexes, it is characterized in that said ultrafiltration system is 10000 daltonian poly (ether sulfone) films.
4. hyclone degerming according to claim 1, remove the mycoplasma complexes, it is characterized in that said poly (ether sulfone) film filtration system is the poly (ether sulfone) film filter, this is that the poly (ether sulfone) film filter is 0.1 micron poly (ether sulfone) film.
5. hyclone degerming according to claim 1, remove the mycoplasma complexes, it is characterized in that the liquid outlet of said reservoir is installed in the bottom of reservoir, liquid outlet is provided with valve; The top of reservoir is equipped with respirator; Be connected with first peristaltic pump between the liquid outlet of reservoir and the liquid inlet of depth filtration systems; Between the liquid outlet of reservoir and first peristaltic pump, and be connected through silica gel hose between the liquid inlet of depth filtration systems and first peristaltic pump.
6. hyclone degerming according to claim 1, remove the mycoplasma complexes, it is characterized in that said gathering system is to stir fluid reservoir; The top of stirring fluid reservoir is equipped with liquid inlet and respirator; The bottom of stirring fluid reservoir is equipped with liquid outlet, and valve is installed on the liquid outlet; This liquid outlet is connected with second peristaltic pump, and liquid outlet is connected with the liquid inlet of second peristaltic pump, and the liquid outlet of second peristaltic pump connects the liquid inlet of finished product bottle placer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220014174XU CN202527079U (en) | 2012-01-13 | 2012-01-13 | Fetal bovine blood removing bacteria and mycoplasma removing assorted device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220014174XU CN202527079U (en) | 2012-01-13 | 2012-01-13 | Fetal bovine blood removing bacteria and mycoplasma removing assorted device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202527079U true CN202527079U (en) | 2012-11-14 |
Family
ID=47128287
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201220014174XU Expired - Fee Related CN202527079U (en) | 2012-01-13 | 2012-01-13 | Fetal bovine blood removing bacteria and mycoplasma removing assorted device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202527079U (en) |
-
2012
- 2012-01-13 CN CN201220014174XU patent/CN202527079U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201626959U (en) | Micro perfusion device for cell culture | |
| US20130115588A1 (en) | Integrated bioreactor and separation system and methods of use therof | |
| JP6474616B2 (en) | One-way separator for holding and recirculating cells | |
| CN108350405A (en) | The active disposable biological processing system of biological support | |
| CN104160013B (en) | Perfusion bioreactor system and the method for operating it | |
| CN103865792B (en) | A kind of circulating fermentable reaction and feed liquid isolation integral equipment | |
| CN112912482A (en) | System and method for preparing biomolecules | |
| CN204727884U (en) | A kind of high-density culture is coupled the film bioreactor of pre-microalgae | |
| CN103756893A (en) | Foam concentration and harvest device for microalgae | |
| CN216337716U (en) | Bioreactor for separating extracellular vesicles | |
| CN202527079U (en) | Fetal bovine blood removing bacteria and mycoplasma removing assorted device | |
| CN115786112A (en) | Differential pressure regulating system, continuous harvesting system and using method thereof | |
| CN207159211U (en) | Microorganism culture concentration filtration system | |
| CN206635330U (en) | A kind of virion antigen purifies concentrator | |
| CN206109422U (en) | Living cells piece -rate system | |
| CN210287374U (en) | Automatic change filterable three-dimensional cell culture device of liquid | |
| CN207031442U (en) | Aerobic fermentation system | |
| CN204550580U (en) | A kind of miniature tangential flow filtration simulator | |
| CN214345637U (en) | A hierarchical extraction element that is arranged in external cell culture liquid skin cell growth factor | |
| CN219991608U (en) | Automatic mass production system for extracellular vesicles | |
| CN100335612C (en) | Two-sectional continuous culturing system of plant cell or tissue | |
| CN212440343U (en) | Aseptic solution conveying pipeline for bio-pharmaceuticals | |
| CN219670522U (en) | Cell culture reactor and culture system | |
| CN215799619U (en) | Cell culture equipment | |
| CN202688239U (en) | Extraction device for monoclonal antibody preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121114 Termination date: 20160113 |