CN203229539U - Multifunctional constant-temperature high pressure hydrostatic pressure loading device in in-vitro cell culture - Google Patents
Multifunctional constant-temperature high pressure hydrostatic pressure loading device in in-vitro cell culture Download PDFInfo
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- CN203229539U CN203229539U CN 201320266685 CN201320266685U CN203229539U CN 203229539 U CN203229539 U CN 203229539U CN 201320266685 CN201320266685 CN 201320266685 CN 201320266685 U CN201320266685 U CN 201320266685U CN 203229539 U CN203229539 U CN 203229539U
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- hydrostatic pressure
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Abstract
The utility model discloses a multifunctional constant-temperature high pressure hydrostatic pressure loading device in in-vitro cell culture. The device is constituted by closed connection of a kettle body and a kettle cover, wherein a compressor interface and a steel cylinder interface are respectively arranged on the two sides of the kettle body through needle valves, and a temperature gauge, a digital induction pressure gauge and an intelligent pressure relief valve are respectively connected above the kettle body by penetrating through the kettle cover. The device can be used for experiments related to high pressure hydrostatic pressure influence in in-vitro cell culture in multiple fields, such as biology and medicine. The device can safely and stably apply hydrostatic pressure to cultured cells and is used for researching the stimulation reactivity of the cells against the hydrostatic pressure of various gases.
Description
Technical field
The utility model relates to a kind of device of biology or medical science, and particularly a kind of multifunction constant temperature cell in vitro is cultivated mesohigh hydrostaticpressure deceleration loading device.
Background technology
Cell cultures is applied to fields such as molecular biology, genetics, immunology, oncology, cell engineering in recent years, develops into a kind of important biomolecule technology, and obtains prominent achievement.
Cell cultures is a kind of program complexity, the more and strict experimental work of condition.Because cell can be subjected to the remarkably influenced of multiple factors such as temperature, nutritive substance, potential of hydrogen, osmotic pressure and microorganism in external growth, breeding, so each link of cell cultures work has strict requirement and regulation as cultivating operations such as vessel cleaning and sterilizing, nutritive medium preparation and degerming, the adjustment of pH value, temperature regulation, will note aseptic technique especially, this is that cells in vitro is cultivated key of success.Therefore not only need the experimenter to the consummation of this technology, also need to have correct scientific theory to instruct and recommendation, and the essential device that relates to is absolutely necessary.
Summary of the invention
The purpose of this utility model is, provides a kind of multifunction constant temperature cell in vitro to cultivate mesohigh hydrostaticpressure deceleration loading device.
In order to realize above-mentioned task, the utility model is taked following technical solution:
A kind of multifunction constant temperature cell in vitro is cultivated mesohigh hydrostaticpressure deceleration loading device, it is characterized in that, by kettle with kettle cover is airtight connects to form, by needle-valve compressor interface and steel cylinder interface are arranged respectively in the kettle both sides, above kettle, pass kettle cover and be connected with temperature measuring gauge, digital induction pressure table and intelligent pressure release valve respectively.
Other characteristics of the present utility model are:
The interior diameter of described kettle is 180mm, and the kettle inner height is 100mm.
Described kettle and kettle cover all adopt the 316L stainless steel to make.
Described temperature measuring gauge is selected digital induction pressure table.
Multifunction constant temperature cell in vitro of the present utility model is cultivated mesohigh hydrostaticpressure deceleration loading device, pressurising scope 0.01mpa～30mpa, and temperature regulating range-20 ℃～100 ℃, pressurized gas is air, carbonic acid gas, nitrogen etc., gas flow rate: 0～1000m
3/ h.Can be used for various biological, the high pressure hydrostaticpressure during the medical science cell in vitro is cultivated influences related experiment.Can safety and stability apply hydrostaticpressure to cultured cells, be used for cell to the research of all kinds of gas hydrostaticpressure irritant reaction.
Description of drawings
Fig. 1 is the structural representation that multifunction constant temperature cell in vitro of the present utility model is cultivated mesohigh hydrostaticpressure deceleration loading device;
Fig. 2 is the kettle cover structural representation of Fig. 1.
Below in conjunction with drawings and Examples the utility model is described in further detail,
Embodiment
Referring to Fig. 1 and Fig. 2, present embodiment provides the concrete structure that a kind of multifunction constant temperature cell in vitro is cultivated mesohigh hydrostaticpressure deceleration loading device, by kettle 1 and kettle cover 8 airtight connecting to form, by needle-valve 4 compressor interface 2 and steel cylinder interface 3 are arranged respectively in kettle 1 both sides, above kettle 1, pass kettle cover 8 and be connected with temperature measuring gauge 5, digital induction pressure table 6 and intelligent pressure release valve 7 respectively.
Between kettle cover 8 and the kettle 1 sealing-ring 9 is arranged, and by circle a fastening screw 10 and 1 airtight connection of kettle, on kettle cover 8, also reserve the opening 11 that temperature measuring gauge 5, digital induction pressure table 6 and intelligent pressure release valve 7 are installed respectively.
During making, the interior diameter of kettle 1 is 180mm, and kettle 1 inner height is 100mm.
Kettle 1 and kettle cover 8 all adopt the 316L stainless steel to make.Wherein, kettle 1 is an integral body, can not weld with stainless steel tube, needle-valve 4 is selected the pressure valve of the 30MPa of Beijing factory production for use, and temperature measuring gauge 5 is selected digital induction pressure table, and temperature measurement accuracy is 0.1 ℃, the design pressure of intelligence pressure release valve 7 is can regulate arbitrarily in 1Mpa～30Mpa, the pressure sharpness of regulation is 0.1Mpa, and compressor interface 2 and steel cylinder interface 3 are standard interface, sealing-ring 9 and fastening screw 10 equal selection standard spares.
The technical parameter of the outer cell cultures mesohigh hydrostaticpressure deceleration loading device of whole functional thermostat is:
Pressurising scope 0.01Mpa～30.00Mpa; Temperature regulation Fan Wei ?20 ℃～100 ℃; Pressurized gas: air, carbonic acid gas, nitrogen etc.; Be suitable for cell culture container: various types of cells culture plate and Tissue Culture Flask etc.; Gas flow rate: 0～1000m
3/ h.
During use, two needle-valves 4 can control respectively that pressurized gas enters and amount discharge container, and pressurized gas enters the transmission of kettle 1 back by nutrient solution to stimulate the hydrostaticpressure that cell applies persistence.Can pass through the Flow-rate adjustment pressing speed, using the temperature of conventional thermostatically heating system held kettle 1 is 37 ℃, can hold various types of cells culture plate and Tissue Culture Flask in the kettle 1, and can put in the common cell culture incubator.
Claims (4)
1. a multifunction constant temperature cell in vitro is cultivated mesohigh hydrostaticpressure deceleration loading device, it is characterized in that, being connected (8) by kettle (1) with kettle cover is airtight forms, by needle-valve (4) compressor interface (2) and steel cylinder interface (3) are arranged respectively in kettle (1) both sides, pass kettle cover (8) in kettle (1) top and be connected with temperature measuring gauge (5), digital induction pressure table (6) and intelligent pressure release valve (7) respectively.
2. multifunction constant temperature cell in vitro as claimed in claim 1 is cultivated mesohigh hydrostaticpressure deceleration loading device, it is characterized in that the interior diameter of described kettle (1) is 180mm, and kettle (1) inner height is 100mm.
3. multifunction constant temperature cell in vitro as claimed in claim 1 is cultivated mesohigh hydrostaticpressure deceleration loading device, it is characterized in that, described kettle (1) and kettle cover (8) all adopt the 316L stainless steel to make.
4. multifunction constant temperature cell in vitro as claimed in claim 1 is cultivated mesohigh hydrostaticpressure deceleration loading device, it is characterized in that, described temperature measuring gauge (5) is selected digital induction pressure table.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320266685 CN203229539U (en) | 2013-05-16 | 2013-05-16 | Multifunctional constant-temperature high pressure hydrostatic pressure loading device in in-vitro cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320266685 CN203229539U (en) | 2013-05-16 | 2013-05-16 | Multifunctional constant-temperature high pressure hydrostatic pressure loading device in in-vitro cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203229539U true CN203229539U (en) | 2013-10-09 |
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ID=49285677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320266685 Expired - Fee Related CN203229539U (en) | 2013-05-16 | 2013-05-16 | Multifunctional constant-temperature high pressure hydrostatic pressure loading device in in-vitro cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203229539U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104109627A (en) * | 2014-07-14 | 2014-10-22 | 上海交通大学 | Portable high pressure microorganism transferring and culturing device |
-
2013
- 2013-05-16 CN CN 201320266685 patent/CN203229539U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104109627A (en) * | 2014-07-14 | 2014-10-22 | 上海交通大学 | Portable high pressure microorganism transferring and culturing device |
| CN104109627B (en) * | 2014-07-14 | 2017-01-11 | 上海交通大学 | Portable high pressure microorganism transferring and culturing device |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131009 Termination date: 20190516 |
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| CF01 | Termination of patent right due to non-payment of annual fee |