CN205473826U - A open pressure control cell culture system for glaucoma basic research - Google Patents
A open pressure control cell culture system for glaucoma basic research Download PDFInfo
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- CN205473826U CN205473826U CN201620201377.8U CN201620201377U CN205473826U CN 205473826 U CN205473826 U CN 205473826U CN 201620201377 U CN201620201377 U CN 201620201377U CN 205473826 U CN205473826 U CN 205473826U
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- air pump
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Abstract
The utility model relates to an open pressure control cell culture system for glaucoma basic research, it includes carbon dioxide incubator, blake bottle, manometer, flowmeter, air pump, converter, flow valve, bottle plug, control system, blake bottle, manometer, flowmeter, air pump, converter, flow valve, bottle plug all set up in the carbon dioxide incubator. The utility model discloses a gaseous exchange between blake bottle and the carbon dioxide incubator all at the carbon dioxide incubator, has been guaranteed to the entry end of air pump, the exit end of flow valve, and the experiment condition is more close with the live body condition, the utility model discloses a control system setting just can operate outside the carbon dioxide incubator outside the carbon dioxide incubator the utility model discloses, convenient operation. The utility model discloses an air pump can not be in the load operation state generally speaking, therefore relative energy -saving.
Description
Technical field
This utility model relates to cell culture system, is particularly used for the open of glaucoma basic research
Stress control cell culture system.
Background technology
Intraocular pressure rising is one of feature of glaucoma, is also the principal element causing deterioration of visual function of glaucomatous eyes.
Correlational study it turned out: intraocular pressure raises, fluctuates or persistently raise, and ganglia retinae can be caused thin
The functional lesion of born of the same parents, pigment epithelium cell etc. or death, ultimately result in the forfeiture of visual function.
GLAUCOMA RESEARCH model can be divided into live body glaucoma animal model and in vitro glaucoma model, Hou Zheyou
Pressurization model is cultivated including external Bulbi hypertonia perfusion model and cell.Cell cultivates pressurization model as cell
The GLAUCOMA RESEARCH model of level, its outstanding advantages is: can freely set stress level, it is possible to obtain perseverance
Constant-pressure, it is possible to get rid of the impact of Confounding Factor, and easily carry out big throughput experimentation.
But traditional airtight culture method is persistently to inject gases at high pressure in culture bottle, completely cut off culture bottle with
The exchange of gas in incubator, along with the prolongation of experimental period may cause medium pH value, HCO- 3
Concentration, oxygen partial pressure (pO2), carbon dioxide dividing potential drop (pCO2) change, thus affect the physiology merit of cell
Can, experiment condition is bigger with the difference of condition of living body.
It addition, traditional airtight culture method is persistently to inject gases at high pressure in culture bottle, air pump is persistently located
In the state of running at full capacity, it is unfavorable for energy-conservation.
Utility model content
For solve the problems referred to above, the purpose of this utility model be provide experiment condition close to condition of living body,
Open Stress control cell culture system for glaucoma basic research energy-conservation and easy to use.
For achieving the above object, the technical scheme that this utility model is taked is: for glaucoma basic research
Open Stress control cell culture system, it include CO2 gas incubator, culture bottle, Pressure gauge,
Effusion meter, air pump, converter, flow valve, bottle stopper, control system；Culture bottle, Pressure gauge, flow
Meter, air pump, converter, flow valve, bottle stopper may be contained within CO2 gas incubator；Gas delivery side of pump
End is connected by pipeline with the arrival end of effusion meter；Bottle stopper is arranged at culture bottle bottleneck；The outlet of effusion meter
Hold and inserted in culture bottle through bottle stopper by pipeline；The arrival end of flow valve is inserted through bottle stopper by pipeline
In culture bottle；Manometric probe inserts in culture bottle through bottle stopper；The arrival end of air pump, flow valve
The port of export may be contained within CO2 gas incubator；Air pump is connected with converter；Pressure gauge, effusion meter,
Converter, flow valve are connected with control system by holding wire respectively；Set-up of control system is in carbon dioxide
Outside incubator.
Further, the temperature being connected by holding wire with control system it is provided with in CO2 gas incubator
Meter, heater, such that it is able to keep the constant temperature set in making CO2 gas incubator, test bar
Part is with condition of living body relatively.
Due to the arrival end of air pump, flow valve the port of export all in CO2 gas incubator, it is ensured that cultivate
Gas exchange between bottle and CO2 gas incubator, experiment condition is with condition of living body relatively.
Owing to set-up of control system is outside CO2 gas incubator, just can grasp outside CO2 gas incubator
Make this this utility model, easy to operate.
The beneficial effects of the utility model: be generally not at load operating state due to air pump,
Therefore relative energy-saving.
Accompanying drawing explanation
Fig. 1 is the open Stress control cell culture system for glaucoma basic research of the present utility model
The structural representation of system.
1, CO2 gas incubator；2, culture bottle；3, Pressure gauge；4, effusion meter；5, air pump；6、
Converter；7, flow valve；8, bottle stopper；9, control system.
Detailed description of the invention
Referring to Fig. 1, this utility model is the open Stress control cell training for glaucoma basic research
Support system, comprising: CO2 gas incubator 1, culture bottle 2, Pressure gauge 3, effusion meter 4, air pump 5,
Converter 6, flow valve 7, bottle stopper 8, control system 9.
Culture bottle 2, Pressure gauge 3, effusion meter 4, air pump 5, converter 6, flow valve 7, bottle stopper 8 are equal
It is arranged in CO2 gas incubator 1.The port of export of air pump 5 passes through pipeline with the arrival end of effusion meter 4
Connect；Bottle stopper 8 is arranged at culture bottle 2 bottleneck；The port of export of effusion meter 4 passes bottle stopper 8 by pipeline
Insert in culture bottle 2；The arrival end of flow valve 7 is inserted in culture bottle 2 through bottle stopper 8 by pipeline；
The probe of Pressure gauge 3 inserts in culture bottle 2 through bottle stopper 8；The arrival end of air pump 5, flow valve 7
The port of export may be contained within CO2 gas incubator 1.
Air pump 5 is connected with converter 6, can regulate the rotating speed of air pump 5 by converter 6 thus regulate
The flow of air pump 5.
Pressure gauge 3, effusion meter 4, converter 6, flow valve 7 are respectively by holding wire and control system 9
It is connected；Control system 9 is arranged at outside CO2 gas incubator 1.
Use this utility model time, opening control 9, control converter 6 by control system 9 thus
Control air pump 5 start running, gas enters from the arrival end of air pump 5 and passes sequentially through, effusion meter 4, train
Support bottle 2, flow valve 7 and flow out from the port of export of flow valve 7；Make culture bottle 2 and CO2 gas incubator
1 carries out gas exchange.
After this utility model begins to use, control system 9 shows force value that Pressure gauge 3 detected,
The flow value that the set flow value of flow valve 7, effusion meter 4 are detected.Controlled by control system 9
Converter 6 processed thus control air pump 5 operating (unless air pressure in required culture bottle 2 is very big,
Needing air pump 5 prerotation at full capacity, generally air pump 5 is not at running at full capacity state), coordinate
The flow value that reading flow gauge 4 is detected, can control the flow by effusion meter 4；By control it is
System 9 can control the flow by flow valve 7.As required, control respectively by effusion meter 4 flow,
By the flow of flow valve 7, the air pressure in culture bottle 2 can be regulated (according to Pascal's law, open
Pressure in container and inlet gas pressure, flow are proportional, are inverse ratio with outlet gas pressure, flow),
I.e. air pressure in culture bottle 2 can be controlled by control system 9.
Due to the arrival end of air pump 5, flow valve 7 the port of export all in CO2 gas incubator 1, it is ensured that
Gas exchange between culture bottle 2 and CO2 gas incubator 1, experiment condition compares with condition of living body and connects
Closely.
Owing to control system 9 is arranged at outside CO2 gas incubator 1, outer just in CO2 gas incubator 1
This this utility model can be operated, easy to operate.
Owing to air pump 5 is generally not at load operating state, relative energy-saving.
Further, it is also possible to be provided with in CO2 gas incubator 1 and pass through signal with control system 9
The thermometer of line connection, heater, such that it is able to keep the perseverance set in making CO2 gas incubator 1
Temperature condition, experiment condition is with condition of living body relatively.
Above detailed description of the invention is only the preferred embodiment of this creation, not in order to limit this creation,
All any modification, equivalent substitution and improvement etc. done within this spirit created and principle, all should wrap
Within being contained in the protection domain of this creation.
Claims (2)
1. for the open Stress control cell culture system of glaucoma basic research, it is characterised in that: it includes CO2 gas incubator (1), culture bottle (2), Pressure gauge (3), effusion meter (4), air pump (5), converter (6), flow valve (7), bottle stopper (8), control system (9)；Described culture bottle (2), described Pressure gauge (3), described effusion meter (4), described air pump (5), described converter (6), described flow valve (7), described bottle stopper (8) may be contained within described CO2 gas incubator (1)；The port of export of described air pump (5) is connected by pipeline with the arrival end of described effusion meter (4)；Described bottle stopper (8) is arranged at described culture bottle (2) bottleneck；In the port of export of described effusion meter (4) inserts described culture bottle (2) by pipeline through described bottle stopper (8)；In the arrival end of described flow valve (7) inserts described culture bottle (2) by pipeline through described bottle stopper (8)；In the probe of described Pressure gauge (3) inserts described culture bottle (2) through described bottle stopper (8)；The arrival end of described air pump (5), the port of export of described flow valve (7) may be contained within described CO2 gas incubator (1)；Described air pump (5) is connected with described converter (6)；Described Pressure gauge (3), described effusion meter (4), described converter (6), described flow valve (7) are connected with described control system (9) by holding wire respectively；Described control system (9) is arranged at described CO2 gas incubator (1) outward.
2. the open Stress control cell culture system for glaucoma basic research as claimed in claim 1, it is characterised in that: it is provided with the thermometer being connected by holding wire with described control system (9) and heater in described CO2 gas incubator (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620201377.8U CN205473826U (en) | 2016-03-16 | 2016-03-16 | A open pressure control cell culture system for glaucoma basic research |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620201377.8U CN205473826U (en) | 2016-03-16 | 2016-03-16 | A open pressure control cell culture system for glaucoma basic research |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205473826U true CN205473826U (en) | 2016-08-17 |
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ID=56654131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620201377.8U Expired - Fee Related CN205473826U (en) | 2016-03-16 | 2016-03-16 | A open pressure control cell culture system for glaucoma basic research |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205473826U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877214A (en) * | 2021-04-07 | 2021-06-01 | 中南大学湘雅医院 | Pressure controlled cell culture device for glaucoma research |
-
2016
- 2016-03-16 CN CN201620201377.8U patent/CN205473826U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877214A (en) * | 2021-04-07 | 2021-06-01 | 中南大学湘雅医院 | Pressure controlled cell culture device for glaucoma research |
| CN112877214B (en) * | 2021-04-07 | 2023-04-25 | 中南大学湘雅医院 | Pressure control cell culture device for glaucoma research |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20170316 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |