CN202936419U - Pressure and temperature control system for microorganism cultivation - Google Patents
Pressure and temperature control system for microorganism cultivation Download PDFInfo
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- CN202936419U CN202936419U CN 201220655290 CN201220655290U CN202936419U CN 202936419 U CN202936419 U CN 202936419U CN 201220655290 CN201220655290 CN 201220655290 CN 201220655290 U CN201220655290 U CN 201220655290U CN 202936419 U CN202936419 U CN 202936419U
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Abstract
The utility model relates to a pressure and temperature control system for microorganism cultivation. The pressure and temperature control system comprises a box body, a water jacket wrapped on the inner surface of the box body and applicable to provide work temperature for the box body, a device for sampling analysis of gas in the box body, and a gas supply and discharge device for supplementing and releasing gas in the box body. By adopting the technical scheme, the pressure and temperature control system can provide a growth cultivation environment for microorganism under high pressure.
Description
Technical field
The utility model relates to the microorganism culturing field, relates in particular to a kind of pressure, temperature controlling system of microorganism culturing.
Background technology
Microorganism culturing mainly refers to the cultivation to virus, bacterium, actinomycetes, fungi etc., is widely used in the scientific researches such as environment protection, health and epidemic prevention, farming animals, medicine inspection, aquatic products, universities and colleges' experiment and production department.The general condition of microorganism culturing comprises: 1) temperature, 2) the pH value, 3) nutritive substance, 4) pressure.For the control of first three condition, prior art all has some correlation techniques to propose, and take virus culture as example, suitable temperature environment can realize by type of heating; Biological constantly oxygen intake gives off carbon dioxide, and the pH value reduction that this carbonic acid gas gathers together and can cause substratum is unfavorable for biological growth, thereby, can realize by constantly regulating the interior oxygen concentration of biological culture casing and gas concentration lwevel; About nutritive substance, generally realize by pass into nitrogen in cultivating casing.
Yet for the 4th condition, existing microbiological incubator generally is used for providing normal pressure (0.1Mpa left and right) lower culture environment, for the microorganism growing state of (2.5Mpa left and right) under high pressure, requires study.
Based on this, the utility model proposes a kind of pressure, temperature controlling system of microorganism culturing, so that microorganism grown cultures environment under high pressure to be provided.
The utility model content
The purpose that the utility model is realized is pressure, the temperature controlling system that proposes a kind of microorganism culturing, so that microorganism grown cultures environment under high pressure to be provided.
For achieving the above object, the utility model provides a kind of pressure, temperature controlling system of microorganism culturing, comprising:
Casing;
The water jacket of described casing working temperature is provided, and described water jacket is coated on described interior;
Gas in described casing is carried out the device of sampling analysis and the gas in casing replenished the gas barrier that supplies that discharges.
Alternatively, the pressure of described microorganism culturing, temperature controlling system also comprise:
Be arranged on the heating unit of described casing and described water jacket outside;
Be arranged on the pipeline between described heating unit and described water jacket, make the water that heated by heating unit flow into described water jacket, circulation after being flowed back to described heating unit after all release of heat of described water jacket internal recycle one and reheating.
Alternatively, the pressure of described microorganism culturing, temperature controlling system also comprise: be arranged at the water pump on described pipeline.
Alternatively, the current that heated by described heating unit enter described water jacket through after described water pump.
Alternatively, the work of described water pump whether with casing in temperature correlation, pressure, the temperature controlling system of described microorganism culturing also comprise:
Detect the sensor of temperature in casing;
With the temperature control equipment that described sensor, described water pump are connected, whether the work that the temperature signal in the described casing that described temperature control equipment records according to described sensor is controlled described water pump enter the current of described water jacket with adjusting.
Alternatively, the pressure of described microorganism culturing, temperature controlling system also comprise: the fluid infusion case that the current of described circulation (current in pipeline) are carried out supply.
Alternatively, the pressure of described microorganism culturing, temperature controlling system also comprise: the strainer that is used for filtering current in described pipeline.
Alternatively, the current of the described water jacket of outflow enter described heating unit after filtering through described strainer.
Alternatively, the pressure of described microorganism culturing, temperature controlling system also comprise: the rinse mouth that is used for emitting current in described pipeline.
Alternatively, the device of described sampling analysis comprises:
Gas analyzer;
Be arranged on the first valve between described gas analyzer and described casing;
Be arranged on the second valve between described gas analyzer and atmosphere.
Alternatively, described the first valve is needle type valve, and the second valve is needle type valve.
Alternatively, pressure, the temperature controlling system of microorganism culturing also comprise: oxygen pressure reducer and under meter, the gas in the casing of described the first valve of process enters described gas analyzer through oxygen pressure reducer, under meter successively with the atmosphere of described the second valve of process.
Compared with prior art, the utlity model has following advantage:
1) the utility model adopts water jacket to be coated on interior, utilize the water cycle in water jacket to flow, like this by providing heat just can keep the temperature of casing to the current in water jacket, in addition, under hyperbaric environment, for keeping required constant pressure, the gas concentration of microorganism culturing in casing, unavoidably temperature in casing is impacted, the scheme of water jacket is set in interior, by the flash heat transfer of water jacket wall, can makes up delicately these temperature variation.in addition, because microorganism is comparatively responsive to carbonic acid gas and oxygen concentration, thereby, the accurate degree of control to both concentration under high pressure is higher than requiring under normal pressure, thereby, also different from prior art to the device and method that the detection of both gas concentrations is required, particularly, the technical solution of the utility model is: the pressure of described microorganism culturing, temperature controlling system also comprises: the gas in described casing is carried out the device of sampling analysis and the gas in casing replenished the gas barrier that supplies that discharges, oxygen and carbonic acid gas in described sampling and analyzing device selective analysis gas.
2) in possibility, adopt heating unit to realize providing heat to the current in water jacket, this heating unit is arranged on described casing and described water jacket is outside, and between described heating unit and described water jacket, pipeline is set, the current that heated by heating unit flow into water jacket along pipeline, circulation after being able to flow back to along this pipeline described heating unit after all release of heat of described water jacket internal recycle one and reheating.
3) in possibility, for driving flow, on this pipeline, water pump is set.
4) in possibility, the work of described water pump whether with casing in temperature correlation, and the pressure of described microorganism culturing, temperature controlling system also comprise: the sensor and the temperature control equipment that is connected with described sensor, described water pump that detect temperature in casing; Temperature signal in the described casing that described temperature control equipment records according to described sensor is controlled the working order of described water pump, if described temperature is lower than the required temperature of microorganism culturing under described high pressure, described water pump enters operating mode, makes current enter described water jacket; If described temperature is not less than the required temperature of described high pressure microorganism culturing, described water pump enters rest mode, and cut-off heating unit current out enter described water jacket.
In 5 possibilities, for avoid water in working cycle owing to revealing or the reason such as evaporation tails off gradually, pressure, the temperature controlling system of microorganism culturing of the present utility model also comprise: the current of described circulation are carried out the fluid infusion case of supply, this fluid infusion case for example is connected with running water pipe.
6) in possibility, for avoiding ducted contaminated water flow water jacket, pressure, the temperature controlling system of described microorganism culturing also comprise: the strainer that is used for filtering current in described pipeline.
7) in possibility, for satisfying under some demand, need ducted current emptying, pressure, the temperature controlling system of described microorganism culturing also comprise: the rinse mouth that is used for emitting current in described pipeline.
8) in possibility, the device of described sampling analysis comprises: gas analyzer, be arranged on the first valve between described gas analyzer and described casing and be arranged on the second valve between described gas analyzer and ambient atmosphere; Make when sampling, at first close described the first valve, open described the second valve, make atmosphere enter described gas analyzer, the oxygen that described gas analyzer obtains and gas concentration lwevel and with its standard value as the required concentration of microorganism culturing gas under high pressure; Then close described the second valve, open described the first valve, make the gas in described casing enter described gas analyzer, described gas analyzer is measured oxygen and the gas concentration lwevel in described casing; If the oxygen in the casing that records and gas concentration lwevel value and described standard value are inconsistent, gas supply-discharge system is controlled flow/ratio that each gas enters casing and is regulated.
In addition, when gas supply-discharge system is controlled the flow that each gas enters casing/ratio, because air feed is generally high pressure gas (10Mp left and right), meeting swelling heat absorption in the time of in the casing that enters relatively low high pressure (2.5Mp), cause temperature decline in casing, at this moment, temperature controlling system also need strengthen flow velocity in water jacket.
9) in possibility, excessive for avoiding adopting common valve gas to flow into the too fast gaseous tension that causes in casing, described the first valve and the second valve all adopt needle type valve, and the atmosphere through the gas in the casing of described the first valve and described the second valve of process enters described gas analyzer through oxygen pressure reducer, under meter successively, controls with the amount to sample gas.
Description of drawings
Fig. 1 is the pressure of the microorganism culturing that provides of the utility model embodiment, the principle of work schematic diagram of temperature controlling system;
Fig. 2 is the schematic diagram of gas supply-discharge system of pressure, the temperature controlling system of the microorganism culturing that provides of the utility model embodiment;
Fig. 3 is the schematic diagram of sampling and analyzing device of pressure, the temperature controlling system of the microorganism culturing that provides of the utility model embodiment;
Fig. 4 is the schematic diagram of temperature controlling system of pressure, the temperature controlling system of the microorganism culturing that provides of the utility model embodiment.
Embodiment
Just as stated in the Background Art, provide the mode of the microorganism culturing temperature environment under normal pressure for adopting the resistance wire direct heating, and this mode is easily blasted under hyperbaric environment, for avoiding above-mentioned situation, the utility model adopts water jacket to be coated on interior, utilize the water cycle in water jacket to flow, like this by providing heat just can keep the temperature of casing to the current in water jacket.
For above-mentioned purpose of the present utility model, feature and advantage can more be become apparent, below in conjunction with accompanying drawing, embodiment of the present utility model is described in detail, owing to focusing on that principle of the present utility model is described, so drawing not in scale.
Understand for convenience the technical solution of the utility model, below list all Reference numerals that the utility model occurs.
Casing 1 water jacket 2
Hot water inlet 21 hot water outlets 22
The second valve 162 oxygen pressure reducers 163
Under meter 164 gas analyzers 165
Strainer 37
The pressure of paper microorganism culturing, the structure of temperature controlling system.
Figure 1 shows that the pressure of the microorganism culturing that the utility model embodiment provides, the principle of work schematic diagram of temperature controlling system.With reference to shown in Figure 1, pressure, the temperature controlling system of this microorganism culturing comprise:
Casing 1;
The water jacket 2 of described casing 1 working temperature is provided, and described water jacket 2 is coated on the internal surface of described casing 1;
Gas in described casing 1 is carried out the device of sampling analysis and the gas in casing 1 replenished the gas barrier that supplies that discharges.
Shown in Figure 1, particularly, leave a plurality of interfaces on this casing 1, for example relief valve port 11, sewage draining exit 12, inlet mouth 13, venting port 14, two pressure tap 15, thief hatch 16, two cable port (showing in Fig. 1).Above-mentioned each mouthful is used for being connected with corresponding pipe penetration piece, and for example relief valve port 11 is connected with safety valve (being for example stopping valve), opens greater than certain value when 2.5Mpa (for example) for pressure in the cabin; Sewage draining exit 12 is connected with wash water valve, is for example ball valve, is used for the ponding that discharging is positioned at the bottom of casing 1 inside.
Need to prove, in the present embodiment, also reserved an interface (not showing in Fig. 1) on casing 1, in order to other purposes.In other embodiments, this interface also can be reserved several more.
Leave a plurality of interfaces on water jacket 2, be respectively a hot water inlet 21, hot water outlet 22, a venting port (not shown) and a rinse mouth (not shown).
Above-mentioned hot water inlet 21, hot water outlet 22, venting port, rinse mouth for example can be ball valve.
Need to prove, be arranged on the inwall of casing 1 due to water jacket 2, the inwall of both shared casings 1, and the utility model adopts introduces water water jacket 2 after casing 1 and water jacket 2 indirect heating, thereby hot water inlet 21, hot water outlet 22, venting port, rinse mouth all can be arranged on the inwall of shared casing 1.
Then introduce gas supply-discharge system.
With reference to shown in Figure 2, airing system comprises three external gas sources (nitrogen, oxygen, carbonic acid gas) and an air source.After mixing, above-mentioned four sources of the gas enter inlet mouth 13, a stopping valve 131 and a tensimeter 132 are set respectively between each source of the gas and inlet mouth 13, this stopping valve 131 is check valves, and this check valve is automatically opened and closed according to self valve seat both sides gas pressure intensity magnitude relationship.
Exhaust system comprises the stopping valve 141 that arranges on venting port 14 and pipeline that atmosphere is connected, is used for that time opens automatically greater than to a certain degree (for example being 2.5MPa) at casing 1 internal pressure, other situation autoshutdown.
Then introduce sampling and analyzing device.
With reference to shown in Figure 3, mainly to testing with the concentration of the closely-related oxygen of microorganism culturing and carbonic acid gas, concrete instrument is for example MSA gas analyzer 165 to this employing analytical equipment.In addition, the first valve 161 is set between thief hatch 16 and gas analyzer 165, the second valve 162 is set between gas analyzer 165 and atmosphere, to control respectively atmosphere or the interior gas of casing 1 enters gas analyzer 165.
For avoiding the interior gas of casing 1 because pressure is excessive, adopt common valve gas to flow into too much, thereby, described the first valve 161 and the second valve 162 all adopt needle type valve, and enter described gas analyzer 165 through oxygen pressure reducer 163, under meter 164 successively through the atmosphere of the gas in the casing 1 of described the first valve 161 and described the second valve 162 of process.During sampling, at first close described the first valve 161, open described the second valve 162, make atmosphere enter described gas analyzer 165 by described oxygen pressure reducer 163, under meter 164 successively, the oxygen that described gas analyzer 165 obtains and gas concentration lwevel are as standard value (namely with the oxygen in atmosphere and gas concentration lwevel as the required concentration of microorganism culturing oxygen and carbonic acid gas under high pressure); Then close described the second valve 162, open described the first valve 161, make the gas in described casing 1 enter described gas analyzer 165 by described oxygen pressure reducer 163, under meter 164 successively, described gas analyzer 165 is measured oxygen and the gas concentration lwevel in described casing 1.Oxygen in this casing 1 and gas concentration lwevel and both standard values relatively after, judge whether airing system needs air feed.
Be understandable that, above-mentioned in order to obtain the concentration of the interior microorganism culturing desired gas of casing 1, for example oxygen and gas concentration lwevel, need Real-time Collection gas, causes the interior pressure subtle change of casing 1, in addition, microorganism also can consume some gases in process of growth, discharge other gas, these all can cause the interior pressure of casing 1 to change, and this is unfavorable to microorganism culturing under high pressure.In order to keep constant hyperbaric environment (for example 2.5Mp), thereby need to start at any time gas supply-discharge system, in the tonifying Qi process, because external gas source generally is stored in hyperbaric oxygen bottle (10Mp), when entering relatively low high pressure (2.5Mp) by this high pressure, this gas meeting swelling heat absorption causes the interior temperature step-down of casing 1.Temperature regulation in this casing 1 is realized by following temperature controlling system.
Introduce afterwards temperature-control device.
With reference to shown in Figure 4, this temperature-control device comprises:
Be arranged on the heating unit 31 of described casing 1 and described water jacket 2 outsides;
Be arranged on the pipeline 32 between described heating unit 31 and described water jacket 2, make the current that heated by heating unit 31 flow into described water jackets 2 along described pipeline 32, be able to circulation after flow back to described heating unit 31 and reheat after the described water jacket 2 all release of heat of internal recycle one;
Be arranged at the water pump 33 on described pipeline 32, entered described water jacket 2 by the current of described heating unit 31 heating through after described water pump 33;
Detect the sensor 34 of casing 1 interior temperature;
The temperature control equipment 35 that is connected with described sensor 34, described water pump 33, whether the work of the described water pump 33 of temperature signal control that the described casing 1 that described temperature control equipment 35 records according to described sensor 34 is interior, enters the current of described water jacket 2 with adjusting.
In the present embodiment, this heating unit 31 is nichrome wire.
In addition, pressure, the temperature controlling system of described microorganism culturing also comprise: the current to described circulation carry out the fluid infusion case 36 of supply and are used for filtering the interior current of described pipeline 32, prevent that impurity from entering the strainer 37 of water jacket 2.Preferably, the current of the described water jacket 2 of outflow enter described heating unit 31 after filtering through described strainer 37.
The utility model also provides the pressure of mentioned microorganism cultivation, the temperature-controlled process of temperature controlling system, comprising:
If described temperature is lower than the required temperature of microorganism culturing under described high pressure, temperature control equipment 35 is controlled described water pump 33 and is entered operating mode, makes current enter described sleeve pipe 2; If described temperature is not less than the required temperature of microorganism culturing under described high pressure, temperature control equipment 35 is controlled described water pump 33 and is entered rest (treating work) pattern, and cut-off heating unit 31 current out enter described sleeve pipe 2.
Except the said temperature control method, the utility model also provides the pressure of mentioned microorganism cultivation, the pressure control method of temperature controlling system, comprise: required concentration is not inconsistent if gas analyzer 165 is measured the interior oxygen of described casing 1 and/or gas concentration lwevel and described high pressure microorganism culturing, start gas supply-discharge system, after starting gas supply-discharge system, sensor 34 detects the interior temperature variation of casing 1, whether temperature control equipment 35 control water pumps 33 start working, and enter the current of water jacket 2 with adjusting.
Then introduce electrical system.
The native system power supply is AC220V 50HZ, and through isolating switch, alternating current contactor is to each electrical appliance, and for example temperature control equipment 35, heating unit 31, water pump 33, MSA gas analyzer 165 are powered.
At last, for the ease of centralized Control, the present embodiment has proposed the control device of said heating system, sampling and analyzing device and gas supply-discharge system is incorporated on supervisory control desk.
This supervisory control desk can be arranged on the below of casing 1, connects by bracing or strutting arrangement between the two.A plurality of instrument are set on this supervisory control desk, below introduce in detail each instrument.
1. power supply is indicated and key switch: for guaranteeing Electrical Safety and convenient, supervisory control desk is provided with power supply indication and key switch, opens key switch, switches on power, and pilot lamp is lighted.
2. temperature indicator: set 1 digital readout temperature controller (being connected with sensor 34) on supervisory control desk, in order to indicate the temperature in casing 1.
3. gas analyzer (O
2, CO
2): be equipped with 1 MSA type digital display gas analyzer (O on supervisory control desk
2, CO
2), for detection of oxygen concn and the gas concentration lwevel of casing 1 interior environmental gas, when measured medium oxygen concn or gas concentration lwevel reached set(ting)value, the acousto-optic guard signal showed.
4. under meter: be equipped with a under meter on supervisory control desk, be used for test sample analysis of pipeline gas flow after decompression.
5. tensimeter: be equipped with 4 bleed pressure tables on supervisory control desk, measure respectively four inlet mouths 13 input pressure separately.Also be equipped with 2 precision pressure gauges on supervisory control desk, be connected with two pressure tap 15 respectively, be used for measuring simultaneously casing 1 internal pressure.Tensimeter should be arranged in the position of being convenient to observe.
6. supply the bleeding valve: be equipped with 4 DN10 helium oxygen stopping valve and 1 stopping valve on supervisory control desk, wherein 4 helium oxygen stopping valve are controlled three external gas sources (nitrogen, oxygen, carbonic acid gas) and air source air feed in the casing, and it is interior to the outer exhaust of casing 1 that stopping valve is controlled casing 1.
In addition, the electrical control in the present embodiment can be controlled by the key switch on operator's console by the general supply of whole system, makes each electrical appliance control energising or outage by twist switch.
Although the utility model with preferred embodiment openly as above; but it is not to limit the utility model; any those skilled in the art are not within breaking away from spirit and scope of the present utility model; can utilize method and the technology contents of above-mentioned announcement to make possible change and modification to technical solutions of the utility model; therefore; every content that does not break away from technical solutions of the utility model; to any simple modification, equivalent variations and modification that above embodiment does, all belong to the protection domain of technical solutions of the utility model according to technical spirit of the present utility model.
Claims (10)
1. the pressure of a microorganism culturing, temperature controlling system, is characterized in that, comprising:
Casing;
The water jacket of described casing working temperature is provided, and described water jacket is coated on described interior;
Gas in described casing is carried out the device of sampling analysis and the gas in casing replenished the gas barrier that supplies that discharges.
2. the pressure of microorganism culturing according to claim 1, temperature controlling system, is characterized in that, also comprises:
Be arranged on the heating unit of described casing and described water jacket outside;
Be arranged on the pipeline between described heating unit and described water jacket.
3. the pressure of microorganism culturing according to claim 2, temperature controlling system, is characterized in that, also comprises: be arranged at the water pump on described pipeline.
4. the pressure of microorganism culturing according to claim 3, temperature controlling system, is characterized in that, also comprises:
Detect the sensor of temperature in casing;
Temperature in the described casing that is suitable for recording according to described sensor is controlled the temperature control equipment of the work of described water pump, and described temperature control equipment is connected with described sensor, described water pump.
5. the pressure of microorganism culturing according to claim 2, temperature controlling system, is characterized in that, also comprises: the fluid infusion case that the current in described pipeline is carried out supply.
6. the pressure of microorganism culturing according to claim 2, temperature controlling system, is characterized in that, also comprises: the strainer that is used for filtering current in described pipeline.
7. the pressure of microorganism culturing according to claim 2, temperature controlling system, is characterized in that, also comprises: the rinse mouth that is used for emitting current in described pipeline.
8. the pressure of microorganism culturing according to claim 1, temperature controlling system, is characterized in that, the device of described sampling analysis comprises:
Gas analyzer;
Be arranged on the first valve between described gas analyzer and described casing;
Be arranged on the second valve between described gas analyzer and atmosphere.
9. the pressure of microorganism culturing according to claim 8, temperature controlling system, is characterized in that, described the first valve is needle type valve, and the second valve is needle type valve.
10. the pressure of microorganism culturing according to claim 8, temperature controlling system, is characterized in that, also comprises: oxygen pressure reducer and under meter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220655290 CN202936419U (en) | 2012-11-28 | 2012-11-28 | Pressure and temperature control system for microorganism cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220655290 CN202936419U (en) | 2012-11-28 | 2012-11-28 | Pressure and temperature control system for microorganism cultivation |
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| Publication Number | Publication Date |
|---|---|
| CN202936419U true CN202936419U (en) | 2013-05-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220655290 Expired - Fee Related CN202936419U (en) | 2012-11-28 | 2012-11-28 | Pressure and temperature control system for microorganism cultivation |
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| Country | Link |
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| CN (1) | CN202936419U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195041A (en) * | 2014-07-10 | 2014-12-10 | 上海交通大学 | Standing-type electrically-controlled high temperature high pressure microbial cultivation device |
| CN105754852A (en) * | 2016-04-21 | 2016-07-13 | 东北石油大学 | Equipment and measuring method for simulating growth curves of microorganisms in high-temperature and high-pressure oil reservoirs |
-
2012
- 2012-11-28 CN CN 201220655290 patent/CN202936419U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195041A (en) * | 2014-07-10 | 2014-12-10 | 上海交通大学 | Standing-type electrically-controlled high temperature high pressure microbial cultivation device |
| CN105754852A (en) * | 2016-04-21 | 2016-07-13 | 东北石油大学 | Equipment and measuring method for simulating growth curves of microorganisms in high-temperature and high-pressure oil reservoirs |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130515 Termination date: 20171128 |