CN208756252U - A kind of system using carbon dioxide narcosis drosophila - Google Patents
A kind of system using carbon dioxide narcosis drosophila Download PDFInfo
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- CN208756252U CN208756252U CN201721673448.5U CN201721673448U CN208756252U CN 208756252 U CN208756252 U CN 208756252U CN 201721673448 U CN201721673448 U CN 201721673448U CN 208756252 U CN208756252 U CN 208756252U
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- carbon dioxide
- anesthesia
- drosophila
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- bottle
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Abstract
The utility model relates to a kind of systems using carbon dioxide narcosis drosophila, belong to laboratory drosophila anesthesia outfit technical field, solve the inconvenient technical problem of carbon dioxide narcosis drosophila, solution are as follows: carbon dioxide liquid storage bottle is connected to humidification bottle, humidification bottle is connected to anesthesia plate and free branch end respectively, is separately installed with flow control valve on two air-guide branch pipes；The anesthesia plate anesthesia is provided with concave surface in the middle part of plate upper surface, sieve plate is installed above concave surface, strip through-hole is respectively arranged at left and right sides of concave surface, the through-hole that several apertures are gradually increased is provided with by the proximate of air inlet between the strip through-hole of two sides on concave surface, the end of the free branch end is detachably provided with syringe needle.This system combines in culture bottle and anesthesia plate in set of system, has saved the economic cost with carbon dioxide narcosis agent anesthesia drosophila, while being avoided that and occupying too many lab space, and operation is more easy, improves working efficiency.
Description
Technical field
The utility model belongs to laboratory drosophila anesthesia outfit technical field, in particular to a kind of to use carbon dioxide narcosis fruit
The system of fly.
Background technique
Drosophila is classical mode biology, has that the short period, easy raising, at low cost, breeding is fast, individual small, Hou Daiduo, dyeing
The advantages such as body is few, character is obvious, genetic homology, be widely used in science of heredity and auxology correlative study, Parkinson's disease and Ah
The research and development of the nervous system disease agents such as Alzheimer's disease, drug habit, aging and service life, sleep and biological rhythm, learning and memory
Etc. research fields.
In process of scientific research, it is often necessary to go out to meet the drosophila of experiment condition according to the trait segregation of drosophila, this process
It needs to anaesthetize drosophila.Carbon dioxide narcosis method is drosophila anesthesia more mature at present, and operating process is as follows: with two
It after carbonoxide drosophila anaesthetic rifle anaesthetizes the drosophila in culture bottle, then pours them on carbon dioxide narcosis plate, in microscope
Lower carry out relevant operation.Higher cost, required pipeline and relevant device needed for the carbon dioxide narcosis rifle that laboratory uses at present
More, occupancy lab space is larger, and operating method is more complicated.
When carbon dioxide drosophila anaesthetic rifle uses a carbon dioxide humidifying with the air flue for connecting carbon dioxide narcosis plate
When bottle, in order to guarantee the stabilization and anaesthetic effect of throughput, need to connect in carbon dioxide narcosis rifle hose with anesthesia plate soft
Add a clip on pipe respectively.Current experimental provision has the disadvantage that: leading to anesthesia plate using needing to block when anaesthetic rifle
Carbon dioxide gas stream is then needed to block the carbon dioxide gas stream for leading to anaesthetic rifle when using anesthesia plate, be operated more complicated；With folder
Son control carbon dioxide gas stream, throughput are also not easy to grasp；Clip clamps hose and is frequently necessary to turn up hose, is easily destroyed soft
Pipe causes gas leakage, influences anaesthetic effect；And the drosophila in a culture bottle can only be anaesthetized every time, when in high volume being tested,
It will affect the speed and efficiency of experiment.It, can be into one if a set of air supply system is separately configured to carbon dioxide drosophila anaesthetic rifle
Step increases experimental facilities cost, also occupies excessive lab space.
Utility model content
The purpose of the utility model is to overcome the defects of the prior art, solve carbon dioxide narcosis drosophila in the prior art
The technical problem that inconvenient, anesthetic is easily wasted, experimental facilities takes up a large area, experimental facilities is at high cost, provides one kind
Using the system of carbon dioxide narcosis drosophila.
The utility model is achieved by the following technical programs.
A kind of system using carbon dioxide narcosis drosophila, it include carbon dioxide liquid storage bottle, humidification bottle and anesthesia plate,
In: carbon dioxide liquid storage bottle be connected to by gas-guide tube with the air inlet of humidification bottle, the gas outlet of humidification bottle by air-guide main tube and
The air inlet of threeway is connected to, two gas outlets of threeway by two air-guide branch pipes respectively with the air inlet of anesthesia plate and free divide
Branch end is connected to, and is separately installed with flow control valve on two air-guide branch pipes；
The central part of the anesthesia plate is provided with cavity, and the air inlet for anaesthetizing plate is connected to cavity, anaesthetizes plate upper surface
Middle part be provided with concave surface, be covered with the sieve plate with mesh above anesthesia plate, it is logical that bar shaped be respectively arranged at left and right sides of concave surface
Hole, on concave surface between the strip through-hole of two sides by the proximate of air inlet be provided with that several apertures are gradually increased it is logical
Hole；
The end of the free branch end is detachably provided with syringe needle, and the end of the syringe needle is provided with ellipsoid sword
Mouthful, under use state, syringe needle insertion is in the culture bottle of sponge plug, and the distal end of syringe needle is protruded into culture bottle, needle distal end
Side wall is bonded with the inner wall of culture bottle, and the proximal end of syringe needle is set in sponge plug.
Further, switch valve and pressure reducing valve, the gas-guide tube are installed on the gas outlet of the carbon dioxide liquid storage bottle
On pressure gauge and flowmeter are installed.
Further, the material of the sieve plate is plastics, and the aperture of mesh is less than experiment with outside drosophila on the sieve plate
Shape size.
The utility model by adopting the above technical scheme, in drosophila experiment, by the switch valve on carbon dioxide steel cylinder and subtracts
Pressure valve is opened, regulation of carbon dioxide pressure, until showing 5MPa and 0.1MPa in instrument respectively.The gas on carbon dioxide humidification bottle
Flow control valve is opened, and suitable air-flow size is adjusted to.Control valve on carbon dioxide narcosis air-guide branch pipe is closed, dissociates and divides
Flow control valve on Zhi Duan is opened, and for syringe needle plug by the sponge bottle stopper of drosophila culture bottle, carbon dioxide gas stream enters culture bottle.
After drosophila anesthesia, the control valve in free branch end is closed, the flow control valve on carbon dioxide narcosis air-guide branch pipe is beaten
It opens, postanesthetic drosophila is poured out to carbon dioxide narcosis plate.Selecting for drosophila is carried out under the microscope.If needed in experiment
The drosophila in multiple culture bottles is anaesthetized and be selected simultaneously, the throughput on carbon dioxide humidification bottle can be tuned up, by titanium dioxide
Flow control valve in carbon steam hose Liang Ge branch is opened, with faster speed that the drosophila in different culture bottles is numb respectively
It is liquor-saturated to be transferred on anesthesia plate, it can guarantee when anaesthetizing the drosophila in culture bottle, anaesthetize the drosophila on plate also in narcosis, benefit
In subsequent experiment.
The utility model has the advantages that compared with prior art.
1, this system combines in the drosophila on the drosophila anaesthetized in culture bottle and anesthesia plate in set of system, set of system two
Kind use；2, this system has saved the warp with carbon dioxide narcosis agent anesthesia drosophila compared with traditional carbon dioxide narcosis rifle
Ji cost；3, drosophila is anaesthetized using the device, operates more easy, saving manpower, while be avoided that and occupying too many experiment sky
Between；4, using the flow control valve of medical vein syringe, the size of carbon dioxide gas flow can preferably be controlled；5, it eliminates
Hose is turned up and reuses the operation that clip blocks air-flow, avoids gas leakage caused by due to hose damage, save the cost is also protected
Preferable anaesthetic effect is hindered；6, need to anaesthetize the drosophila in multiple culture bottles, two air-guide branch pipes can be opened simultaneously and be controlled
Throughput, the drosophila in anesthesia culture bottle simultaneously, can guarantee that the drosophila on anesthesia plate is in narcosis, improve experiment effect
Rate saves time cost；7, the free branch end of carbon dioxide steam hose is designed to hard, the syringe needle with ellipsoid cutting edge,
Be conducive to the insertion needle in the gap of bottle stopper and bottleneck, and not will form big gap, while anaesthetizing drosophila, avoids
Drosophila flies out culture bottle；8, the free branch design position of carbon dioxide steam hose is slightly remote from anesthesia plate, will not influence drosophila
Separation and select work, improve working efficiency.
Detailed description of the invention
Fig. 1 is the utility model overall structure diagram.
Fig. 2 is anesthesia plate schematic perspective view.
Fig. 3 is the overlooking structure diagram that plate is anaesthetized in Fig. 2.
Fig. 4 is the schematic diagram of main cross-sectional structure of anesthesia plate and sieve plate in use state in Fig. 2.
Fig. 5 is the broken section structural schematic diagram of syringe needle in a state of use.
In figure, 1 is carbon dioxide liquid storage bottle, and 2 be humidification bottle, and 3 be threeway, and 4 be flow control valve, and 5 be anesthesia plate, and 51 are
Air inlet, 52 be strip through-hole, and 53 be concave surface, and 54 be through-hole, and 55 be sieve plate, and 6 be free branch end, and 61 be culture bottle, and 62 are
Syringe needle, 63 be sponge plug.
Specific embodiment
Elaborate below with reference to embodiment to the utility model: the present embodiment is to be with technical solutions of the utility model
Premise is implemented, and the detailed implementation method and specific operation process are given, but the protection scope of the utility model is unlimited
In the following examples.
As shown in Figures 1 to 5, in the present embodiment
A kind of system using carbon dioxide narcosis drosophila, it includes carbon dioxide liquid storage bottle 1, humidification bottle 2 and anesthesia plate
5, in which: carbon dioxide liquid storage bottle 1 is connected to by gas-guide tube with the air inlet of humidification bottle 2, and the gas outlet of humidification bottle 2 passes through air guide
Supervisor is connected to the air inlet of threeway 3, and two gas outlets of threeway 3 pass through the air inlet with anesthesia plate 5 respectively of two air-guide branch pipes
Mouth is connected to free branch end 6, is separately installed with flow control valve 4 on two air-guide branch pipes；
The central part of the anesthesia plate 5 is provided with cavity, and the air inlet 51 of anesthesia plate 5 is connected to cavity, anaesthetizes on plate 5
Concave surface 53 is provided in the middle part of surface, concave surface 53 gathers carbon dioxide gas physical efficiency preferably in anesthesia 5 upper surface of plate
Middle part avoids the quick diffusion of carbon dioxide gas.The sieve plate 55 with mesh, the sieve plate 55 are covered with above anesthesia plate 5
Material be plastics, the aperture of mesh is less than the outer dimension that drosophila is used in experiment on the sieve plate 55, in experimentation, CO2Gas
Body anaesthetizes drosophila by the mesh on sieve plate 55, and that completes on sieve plate 55 drosophila selects work.The left and right sides of concave surface 53
It is respectively arranged with strip through-hole 52, is set between two sides strip through-hole 52 by the proximate of air inlet 51 on concave surface 53
It is equipped with the through-hole 54 that several apertures are gradually increased, by adjusting the aperture of through-hole 54, makes up 51 distal end CO of air inlet2Gas release
The deficiency that amount is reduced, drosophila easily revives.；
The end of the free branch end 6 is detachably provided with syringe needle 62, and the end of the syringe needle 62 is provided with ellipsoid
Face cutting edge, under use state, for the insertion of syringe needle 62 in the culture bottle 61 of sponge plug 63, culture bottle 61 is protruded into the distal end of syringe needle 62
Interior, the side wall of 62 distal end of syringe needle is bonded with the inner wall of culture bottle 61, and the proximal end of syringe needle 62 is set in sponge plug 63.
Further, switch valve and pressure reducing valve, the gas-guide tube are installed on the gas outlet of the carbon dioxide liquid storage bottle 1
On pressure gauge and flowmeter are installed.
The utility model use process is as follows:
1, switch valve and pressure reducing valve to the instrument opened on carbon dioxide steel cylinder show 5MPa and 0.1MPa；
2, with the control damper regulation of carbon dioxide throughput on carbon dioxide humidification bottle；
3, the flow control valve on anesthesia plate side carbon dioxide narcosis air-guide branch pipe is closed, free branch end side is opened
Flow control valve on air-guide branch pipe；
4, syringe needle is inserted in the sponge bottle stopper of No. 1 drosophila culture bottle, and carbon dioxide gas anaesthetizes drosophila；
5, the flow control valve on anesthesia plate side carbon dioxide narcosis air-guide branch pipe is opened, the drosophila in headpin is fallen
On drosophila anesthesia plate, anaesthetizes the through-hole being arranged on plate and be sequentially increased by proximal end to distal end aperture, ensure that sufficient titanium dioxide
Carbon supply, is conducive to the anesthesia of drosophila；
6, syringe needle is inserted in the sponge bottle stopper of No. 2 drosophila culture bottles, and carbon dioxide gas anaesthetizes drosophila；
7, the flow control valve on anesthesia plate side carbon dioxide narcosis air-guide branch pipe is opened, the drosophila in No. 2 bottles is fallen
On anesthesia plate, the control valve in free branch is closed；
8,4 to 7 are repeated the above steps, the drosophila on aggregation drosophila anesthesia plate carries out subsequent separation and selects work.
Above description is only a specific implementation of the present invention, but the protection scope of the utility model is not limited to
It is any to be familiar with those skilled in the art within the technical scope disclosed by the utility model in this, the variation that can be readily occurred in
Or replacement, it should be covered within the scope of the utility model.Therefore, the protection scope of the utility model should be with the power
Subject to the protection scope that benefit requires.
Claims (3)
1. a kind of system using carbon dioxide narcosis drosophila, it includes carbon dioxide liquid storage bottle (1), humidification bottle (2) and anesthesia
Plate (5), it is characterised in that: carbon dioxide liquid storage bottle (1) is connected to by gas-guide tube with the air inlet of humidification bottle (2), humidification bottle (2)
Gas outlet be connected to the air inlet of threeway (3) by air-guide main tube, two gas outlets of threeway (3) pass through two air-guide branch pipes
It is connected to respectively with the air inlet of anesthesia plate (5) and free branch end (6), is separately installed with flow control valve on two air-guide branch pipes
(4)；
The central part of anesthesia plate (5) is provided with cavity, and the air inlet (51) of anesthesia plate (5) is connected to cavity, anaesthetizes plate
(5) it is provided in the middle part of upper surface concave surface (53), is covered with the sieve plate (55) with mesh above anesthesia plate (5), concave surface (53)
The left and right sides is respectively arranged with strip through-hole (52), by air inlet between two sides strip through-hole (52) on concave surface (53)
(51) proximate is provided with the through-hole (54) that several apertures are gradually increased；
The end of the free branch end (6) is detachably provided with syringe needle (62), and the end of the syringe needle (62) is provided with ellipse
Spherical surface cutting edge, under use state, in the culture bottle (61) of sponge plug (63), the distal end of syringe needle (62) is stretched for syringe needle (62) insertion
Enter in culture bottle (61), the side wall of syringe needle (62) distal end is bonded with the inner wall of culture bottle (61), and the proximal end of syringe needle (62) is set to
In sponge plug (63).
2. a kind of system using carbon dioxide narcosis drosophila according to claim 1, it is characterised in that: the titanium dioxide
Switch valve and pressure reducing valve are installed on the gas outlet of carbon liquid storage bottle (1), pressure gauge and flowmeter are installed on the gas-guide tube.
3. a kind of system using carbon dioxide narcosis drosophila according to claim 1, it is characterised in that: the sieve plate
(55) material is plastics, and the aperture of mesh is less than the outer dimension that drosophila is used in experiment on the sieve plate (55).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721673448.5U CN208756252U (en) | 2017-12-05 | 2017-12-05 | A kind of system using carbon dioxide narcosis drosophila |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721673448.5U CN208756252U (en) | 2017-12-05 | 2017-12-05 | A kind of system using carbon dioxide narcosis drosophila |
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| Publication Number | Publication Date |
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| CN208756252U true CN208756252U (en) | 2019-04-19 |
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| CN201721673448.5U Expired - Fee Related CN208756252U (en) | 2017-12-05 | 2017-12-05 | A kind of system using carbon dioxide narcosis drosophila |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753676A (en) * | 2019-11-04 | 2021-05-07 | 何凤侠 | By CO2Adult device for anaesthetizing and selecting mosquitoes and flies |
-
2017
- 2017-12-05 CN CN201721673448.5U patent/CN208756252U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753676A (en) * | 2019-11-04 | 2021-05-07 | 何凤侠 | By CO2Adult device for anaesthetizing and selecting mosquitoes and flies |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190419 Termination date: 20191205 |