CN203144416U

CN203144416U - Device for separating rare cells in blood

Info

Publication number: CN203144416U
Application number: CN 201320086214
Authority: CN
Inventors: 张晓晶; 沈挺
Original assignee: NINGBO MEIJING MEDICAL TECHNOLOGY Co Ltd
Current assignee: NINGBO MEIJING MEDICAL TECHNOLOGY Co Ltd
Priority date: 2013-02-25
Filing date: 2013-02-25
Publication date: 2013-08-21
Anticipated expiration: 2023-02-25

Abstract

The utility model discloses a device for separating rare cells in blood. The device for separating the rare cells in blood comprises a storage for providing a blood sample, a pump for pumping the blood sample, and a micro chip, wherein a first connecting pipe of the storage is communicated with a blood inlet hole of the micro chip, the pump is communicated with a blood outlet hole of the micro chip by virtue of a second connecting pipe, the micro chip is connected with a rotating mechanism, the rotating mechanism drives the micro chip to deflect, and the deflection center of the micro chip is located on the first connecting pipe and is close to the micro chip. The device for separating the rare cells in blood has the advantages that the rotating center is located on the first connecting pipe and is close to the micro chip, so that the length of the first connecting pipe can be reduced to the greatest extent.

Description

The tripping device of rare cell in a kind of blood

Technical field

The utility model relates to a kind of blood testing device, especially relates to the tripping device of rare cell in a kind of blood.

Background technology

Blood rare cell separometer is the diagnostic tool of future generation that detects rare cell in the blood relevant with major disease, and rare cell comprises cell with specified protein mark of circulating tumor cell (CTC), pernicious stem cell and the pathology of circulation in blood sample etc.Relevant studies confirm that the circulating tumor cell quantity that occurs in the patients'blood sample and patient's early diagnosis and survival rate have very strong dependency.Therefore, rare cell detection and the analysis to the patients'blood sample is the key that improves tumor disease early discovery rate and personalized treatment.

The applying date that is all the applicant's application is on October 15th, 2012, the patent No. is that 201210389753.7 Chinese utility application file discloses " separometer of rare cell in a kind of blood ", its structure is as follows: comprise storer and pump, microchip, microchip comprises thin slice and the slide glass with groove, and thin slice is arranged on and forms a microfluidic channel on the slide glass; This microfluidic channel is connected between this storer and this pump, allows this blood sample from the memory stream to the pump;

A plurality of magnet are positioned at this slide glass bottom, form the Distribution of Magnetic Field of gradient along the length of slide glass, and slide glass is used for gathering the rare cell that microchip is caught.Also comprise the full wind-up for the direction of this microfluidic channel of rotation.Under the driving of full wind-up, this microfluidic channel in the vertical position, level attitude and symmetrical upturned position replace, and can make cellular segregation more effective.This patent document does not disclose the concrete rotating manner of microchip and the peculiar effect of bringing in detail.

Summary of the invention

Technical problem to be solved in the utility model provides the tripping device of rare cell in the better blood of a kind of separating effect.

The utility model solves the problems of the technologies described above the technical scheme that adopts: the tripping device of rare cell in a kind of blood comprises:

The storer that blood sample is provided;

A pump that is used for extracting blood sample;

With a microchip; Storer is communicated with the fluid inlet of microchip by first pipe connecting, and pump is communicated with the liquid outlet of microchip by second pipe connecting,

It is characterized in that described microchip is connected with a rotating mechanism, described rotating mechanism makes microchip produce deflection, and the deflection center of microchip is positioned on first pipe connecting, and this deflection center is near microchip.

Rotating mechanism comprises motor and swivel arm, the described swivel arm of described electric motor driving, and described microchip is fixed on the swivel arm.

Microchip comprises thin slice and the slide glass with groove, and thin slice is provided with fluid inlet and liquid outlet, and thin slice is arranged on and forms a microfluidic channel on the slide glass; Magnet is positioned at this slide glass bottom, and forms the Distribution of Magnetic Field of gradient along the length of slide glass, and slide glass is used for gathering the rare cell that microchip is caught;

Described microchip can be in three kinds of states under the effect of swivel arm; First state: microchip becomes 20-40 degree angle with horizontal plane, magnet is positioned at the top of slide glass, and the liquid outlet of microchip is lower than the fluid inlet of microchip; Second state: microchip becomes horizontality, and the fluid inlet of microchip or liquid outlet are down; The third state: microchip is in plumbness, and the fluid inlet of microchip is higher than liquid outlet.

Compared with prior art, this center of rotation of advantage of the present utility model is positioned on first pipe connecting, and this center of rotation is near microchip, so that shorten the first required pipe connecting length as far as possible.The upset microchip makes microchip be in three different states, first state: the PBS damping fluid is discharged the air in the microchip, second state: allow the blood sample microchip of flowing through, microfluidic channel is positioned at the below of magnet, utilize magnet that the magnetic force of rare cell and the opposite principle of direction of rare cell gravity are separated rare cell this moment from blood sample, the 3rd state: microchip is in plumbness, more be conducive to the user to the fetching of slide glass, three states have been finished the separation to rare cell.

Description of drawings

Fig. 1 is first view of the present utility model;

Fig. 2 is second view of the present utility model;

Fig. 3 is third state synoptic diagram of the present utility model.

Embodiment

Describe in further detail below in conjunction with the utility model of accompanying drawing embodiment.

The tripping device of rare cell in a kind of blood comprises:

The storer 1 that blood sample is provided;

A pump 2 that is used for extracting blood sample;

With a microchip 3; Storer 1 is communicated with the fluid inlet 31 of microchip 3 by first pipe connecting 4, and pump 2 is communicated with the liquid outlet 32 of microchip 3 by second pipe connecting 5,

Microchip 3 is connected with a rotating mechanism, and rotating mechanism makes microchip 3 produce deflection, and the deflection center 6 of microchip 3 is positioned on first pipe connecting 4, and this deflection center 6 is near microchip 3.

Rotating mechanism comprises motor and swivel arm, the electric motor driving swivel arm, and microchip 3 is fixed on the swivel arm.

Microchip 3 comprises thin slice and the slide glass with groove, and fluid inlet 31 and liquid outlet 32 are arranged on the thin slice, and thin slice is arranged on and forms a microfluidic channel on the slide glass; Magnet is positioned at this slide glass bottom, and forms the Distribution of Magnetic Field of gradient along the length of slide glass, and slide glass is used for gathering the rare cell that microchip 3 is caught;

Microchip 3 can be in three kinds of states under the effect of swivel arm; First state: microchip 3 becomes 20-40 degree angle with horizontal plane, magnet is positioned at the top of slide glass, and the liquid outlet 32 of microchip 3 is lower than the fluid inlet 31 of microchip 3; Second state: 3 one-tenth horizontalitys of microchip, the fluid inlet 31 of microchip 3 or liquid outlet 32 are down; The third state: microchip 3 is in plumbness, and the fluid inlet 31 of microchip 3 is higher than liquid outlet 32.

Claims

1. the tripping device of rare cell in the blood comprises:

The storer that blood sample is provided;

A pump that is used for extracting blood sample;

2. the tripping device of rare cell in a kind of blood according to claim 1 is characterized in that rotating mechanism comprises motor and swivel arm, the described swivel arm of described electric motor driving, and described microchip is fixed on the swivel arm.

3. the tripping device of rare cell in a kind of blood according to claim 2 is characterized in that microchip comprises thin slice and the slide glass with groove, and thin slice is provided with fluid inlet and liquid outlet, and thin slice is arranged on and forms a microfluidic channel on the slide glass; Magnet is positioned at this slide glass bottom, and forms the Distribution of Magnetic Field of gradient along the length of slide glass, and slide glass is used for gathering the rare cell that microchip is caught.

4. the tripping device of rare cell in a kind of blood according to claim 3 is characterized in that described microchip can be in three kinds of states under the effect of swivel arm; First state: microchip becomes 20-40 degree angle with horizontal plane, magnet is positioned at the top of slide glass, and the liquid outlet of microchip is lower than the fluid inlet of microchip; Second state: microchip becomes horizontality, and the fluid inlet of microchip or liquid outlet are down; The third state: microchip is in plumbness, and the fluid inlet of microchip is higher than liquid outlet.