CN108546676A - The method that tunnel magnetic field technology detaches and is enriched with rare cell in peripheral blood - Google Patents
The method that tunnel magnetic field technology detaches and is enriched with rare cell in peripheral blood Download PDFInfo
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- CN108546676A CN108546676A CN201810362931.4A CN201810362931A CN108546676A CN 108546676 A CN108546676 A CN 108546676A CN 201810362931 A CN201810362931 A CN 201810362931A CN 108546676 A CN108546676 A CN 108546676A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
Abstract
Rare cell in peripheral blood is detached and is enriched with using tunnel magnetic field technology the invention discloses a kind of（Fetal nucleated red blood, circulating tumor cell etc.）Method, step includes:（a）The magnetic bead for being coated with purpose rare cell specific antibody or aptamer is added in sample peripheral blood and is incubated, cell antibody or aptamer magnetic bead conjugate are formed it into.Aim cell（Fetal nucleated red blood, circulating tumor cell etc.) compared with other haemocytes carry surface antigen it is more or with aptamer affinity it is stronger, to combine greater number of magnetic bead.（b）Blood sample after antibody or aptamer magnetic bead are incubated flows through the film tube in tunnel magnetic field, and the cell for carrying different number magnetic bead rests on the different location of thin film tube wall because of its magnetic induction intensity difference, to reach rare cell（Fetal nucleated red blood, circulating tumor cell etc.) separation effect.（c）Implement washing step, removal does not carry the cell of magnetic bead.（d）It can implement fixation, dyeing and the fluorescent marker of cell in the case where magnetic field is kept；（e）Apply negative pressure to film tube and form it into diaphragm, it is fixedly separated after cell and sealing both ends, to make the subsequent identification parting of cell（Zymetology, morphology and molecular Biological Detection etc.）.Compared with prior art, this method is simple, quick, stable, high degree of automation, and aim cell is lost less, is suitable for scientific research and clinical trial diagnosis.
Description
Technical field
The present invention relates to biomedical sectors, more particularly to a kind of using dilute in tunnel magnetic field separation and enrichment peripheral blood
There is cell（Fetal nucleated red blood, circulating tumor cell etc.) method.
Background technology
In human peripheral blood other than red blood cell, leucocyte and blood platelet etc., it is rare thin that there is likely to be some sometimes
Born of the same parents, as there are include in fetal nucleated red blood and cycle in circulating tumor cell, maternal blood in blood of cancer patients
Chrotoplast etc..These cell quantities are rare（Only contain about 1-10 in 1 milliliter of blood）, but there is important clinical value.Such as cycle
Tumour cell can be used for early diagnosis, prognosis evaluation, therapeutic evaluation and the individualized treatment of tumour；Fetal nucleated red blood is because gathering around
There is complete fetal genetic material, is unanimously considered the most ideal target cell of Non-invasive prenatal diagnosis.
Rare cell in peripheral blood at present（Fetal nucleated red blood, circulating tumor cell etc.) separation and enrichment method master
It is divided into unspecific enrichment method and specific enrichment method.Unspecific enrichment method is special using the physics of cell itself
Property is enriched with.The enrichment method of specificity is enriched with using cell surface Specific marker.Based on principles above
Cell detaches and enrichment method is varied, including density-gradient centrifugation method, cell filtration technology, cell adherence technology, nanometer
Matrix isolation technics, Flow cytometry, microfluidic chip technology and immuno magnetic cell separation technology.Due to aim cell sheet
The physical property (such as size, density and electrochemical properties) of body exists with other blood cells to partly overlap, non-specific richness
Set method is difficult to separate rare aim cell from peripheral blood.Fluidic cell sorting technology is to utilize fluidic cell
Instrument is irradiated under flow at high speed state with high energy laser by the unicellular or particle of fluorochromes, measures dissipating for its generation
It penetrates light and emits the intensity of fluorescence, it is a kind of modern thin to qualitatively or quantitatively be detected to cell quality and functional status etc.
Born of the same parents' analytical technology.This method separation purity is higher, but flow cytometer volume is big, involves great expense, complicated for operation；And it sorted
The parts Cheng Zhongyou cell loss and morphologic change.Microfluidic chip technology is that sample preparation, reaction, separation, detection etc. is basic
Operating unit is integrated on the chip of one piece of micro-meter scale, is automatically performed analysis overall process.Sample size needed for this method is small, separation
Purity is higher, but it there is also some problems in industrialized production and micromation integration etc., and instrument and chip cost are high
It is expensive.
Immuno magnetic cell separation technology is presently the most common rare cell isolation technics, it passes through function base based on antigen
Group be combined with the specific antibody being coated on magnetic bead, formed Ag-Ab-bead complexes, this compound with compared with
High magnetic responsiveness, displacement, makes compound be detached with other substances under the influence of a magnetic field, and reaches separation and concentration cell
Purpose.Existing immuno magnetic cell separation method usually carries out on immuno magnetic cell separation column, and is needed to improve cell purity
Repeatedly rinse（Key player on a team/negative choosing）, it is cumbersome and easily lead to aim cell loss, common micron order magnetic bead is also to a certain degree
On attract each other and squeeze cell and cause the destruction of aim cell, these all influence immuno magnetic cell separation effect, cause it is rare carefully
There are false negative, false positive results for born of the same parents' separation and concentration, and the specificity of testing result and sensitivity is made to be difficult to meet clinical practice work
The needs of work.On the other hand, some rare aim cell surface specific antigens also have a small amount of presence in other cells, this
Also the purity of cell separation is affected.Therefore, rare cell separation of low cost there is an urgent need for a kind of easy to operate, reliable and stable
Method is in laboratory operation and clinical application.
Invention content
In order to overcome the above-mentioned deficiencies of the prior art, existed using antibody or aptamer magnetic bead the present invention provides a kind of
Rare cell in separation and concentration peripheral blood in strong magnetic channel（Fetal nucleated red blood, circulating tumor cell etc.) method.
One, a kind of device being sorted antibody or aptamer magnetic bead-cell conjugate using strong magnetic channel, packet are provided
The tunnel equipment for including the upstream infusion mechanism for providing cleaning solution and sample liquid, generating high-intensity magnetic field（Magnetic field intensity is in 0-3T
Can freely it regulate and control）, cell sorting film tube in strong magnetic channel, be located at magnetic sorting mechanism liquid flow and move downstream
Collecting mechanism and film tube negative pressure device.
Two, aim cell is selected（Fetal nucleated red blood, circulating tumor cell etc.) specificity antibody magnetic bead or nucleic acid
Aptamers magnetic bead（Ensure aim cell surface specific antigen levels and mix cell have larger difference）, this magnetic bead is added anti-
In blood coagulation sample, being placed in be incubated on blending instrument makes magnetic bead be coupled with the cell in peripheral blood, and different cells are because of its surface antigen number
Amount is different or different from the affinity of aptamer and combines the magnetic bead of different number, to which the magnetic induction for being presented different is strong
Degree.
Three, the film tube in strong magnetic channel is flowed through to form lasting mobile phase with constant speed with isotonic solution.By antibody or core
The sour processed peripheral blood sample of aptamers magnetic bead injects film tube in the presence of isotonic solution mobile phase, carries different number magnetic bead
Cell because of its magnetic induction intensity difference, thin film tube wall is adsorbed under the action of high-intensity magnetic field and forms the different cell band of flow path,
The cell for not carrying magnetic bead is flushed film tube under the action of mobile phase, to achieve the effect that rare cell detaches.
Four, when necessary, dyeing liquor or fluorescent marker etc. can be conveyed respectively to film tube, realize cell native staining or
Fluorescent marker, subsequently to identify.
Five, apply negative pressure to infusion pipeline, film tube is made to become diaphragm, the cell at sealing both ends, arrangement is moulded as crossed
It is equally mothballed and is fixed in diaphragm, in case morphology, molecular biology, zymetology etc. are further identified.
Above step operates continuously in order, can be controlled with program, precisely implements.
Compared with prior art, the beneficial effects of the invention are as follows：
1, the cell separation principle used in the present invention is to carry out ladder by the quantity of magnetic bead entrained by cell similar to mass spectrograph
Degree separation, this has widened the selection face to aim cell surface specific antigen, improves the cell purity after separation and enrichment.
Existing Beads enrichment technology is only capable of through cell surface antigen with/without progress key player on a team or negative choosing, and the present invention can be according to cell
Surface antigen it is more/carry out gradient separations less, significantly improve the separation of level of rare cell.
2, existing Beads enrichment technology, separated magnetic cell need to elute in solution, and concentration effect is poor, this is mesh
The problem of preceding technology urgent need to resolve.Rare cell after present invention separation forms cell band and is enriched in vacuum film piece, magnetic bead
It is not required to subsequent processing removal, morphological examination can be directly carried out, the accuracy of cell type identification be significantly improved, after also allowing for
Continuous detection.This is not available for existing method.
3, blood preparation handling and operation process is simple, capture aim cell that can be specific in whole blood, need not picture
The prior art carries out pre-separation to improve separation purity to blood preparation, multi-step detaches, this largely avoids dilute
There is the false negative of cell loss in operation and detection.
4, strong magnetic channel device simple structure of the present invention, it is cheap, it is easy to operate, convenient for industrialized production and greatly
Scale is used in laboratory operation and clinical application.
Specific implementation mode
In order to make the present invention be more clearly understood, with reference to embodiments, the present invention will be described in further detail.It answers
Work as understanding, described herein specific examples are only used to explain the present invention, is not intended to limit the present invention.
Embodiment 1：Fetal nucleated red blood（fNRBC）Isolate and purify
1）Adult heparin anti-coagulating 5ml is taken, the 10 μ l of cord blood sample and antibody magnetic bead liquid of 100 times of normal saline dilutions is added
5ml, test tube, which is set, mixes 30 min on blending instrument.
Explanation：Interior separation for 24 hours, antibody used are mouse anti-human transferrin after adult blood and umbilical cord blood collection（CD71）Dan Ke
Grand antibody, magnetic bead are ferroferric oxide nano granules, diameter 30nm；Magnetic bead and antibody magnetic bead are had by the red certain kind of berries biotechnology in Shenzhen
It is standby to limit corporation.
2）The magnetic field intensity of strong magnetic channel device is adjusted to 2T, the pipeline in tunnel is transparent plastic film pipe.It first will be thin
Membrane tube is using the constant current speed saline injection of 3ml/min as mobile phase, then by 10ml antibody magnetic bead blood samples with 1ml/min's
Constant speed injects mobile phase and all injection film tube flows through strong magnetic channel（Film liquid in pipe overall flow rate is 4ml/min at this time）,
After blood sample is injected, mobile phase maintains flowing at least 3min.
Explanation：The magnetic channel of strong magnetic channel device is Rectangular Tunnel, size 110*5*2mm（Long * wide * high）, in tunnel
Magnetic field is DC electromagnetic field, and perpendicular to the direction that liquid flows, magnetic field intensity is constant adjustable in 0-3T in the direction of the magnetic line of force, this is strong
Magnetic channel device is manufactured and designed by the bio tech ltd Hong Mei of Shenzhen；The tube wall of plastic film tube is 0.1mm in tunnel,
Bore is 3mm；The pipeline for connecting film tube both ends is medical infusion lines.
3）Stop mobile phase, magnetic field inputs methanol, 30% Rui Shi dyes with the constant current speed of 3ml/min respectively successively under maintaining
Color liquid and each 15ml of PBS buffer solution.
4）Pipeline, which vacuumizes, makes film tube shrink to form diaphragm, seals the both ends of diaphragm, and diaphragm is taken out in shearing.
Diaphragm is placed in microscopy on glass slide.
The result shows that 1）The magnetic bead that endochylema dyes red erythroblast combination is most, is located at the left end of diaphragm
（Blood sample arrival end）, it is kept completely separate with granulophilocyte and remaining leucocyte, the blood platelet and Magnetic particles etc. positioned at the right, at
Ripe red blood cell is in film tube almost without residual；2）With obtained by microscopic counting on diaphragm fNRBC quantity divided by blood sample in
The bleeding of the umbilicus fNRBC theoretical values of addition obtain the rate of recovery, then the rate of recovery of this experiment erythroblast is 98%.
Embodiment 2：Circulating tumor cell（circulating tumor cell, CTC）Isolate and purify
1）Adult heparin anti-coagulating 5ml is taken, 10 μ l of human cervical carcinoma cell suspension are added（Containing 50 Hela cell）And antibody magnetic
Pearl liquid 5ml, test tube, which is set, mixes 30 min on blending instrument.
2）The magnetic field intensity of strong magnetic channel device is adjusted to 2T, the pipeline in tunnel is transparent plastic film pipe.It first will be thin
Membrane tube is using the constant current speed saline injection of 3ml/min as mobile phase, then by 10ml antibody magnetic bead blood samples with 1ml/min's
Constant speed injects mobile phase and all injection film tube flows through strong magnetic channel（Film liquid in pipe overall flow rate is 4ml/min at this time）,
After blood sample is injected, mobile phase maintains flowing at least 3min.
Explanation：Strong magnetic channel device is the same as embodiment 1.
3）Stop mobile phase, magnetic field inputs methanol, 30% Rui Shi dyes with the constant current speed of 3ml/min respectively successively under maintaining
Color liquid and each 15ml of PBS buffer solution.
4）Pipeline, which vacuumizes, makes film tube shrink to form diaphragm, seals the both ends of diaphragm, and diaphragm is taken out in shearing.It is thin
Diaphragm is placed in microscopy on glass slide.
The result shows that 1）The magnetic bead of Hela cell combinations is most, is located at the left end of diaphragm（Blood sample arrival end）, with position
It is kept completely separate in a small amount of normal peripheral blood cell on the right and Magnetic particles etc.；2）With the Hela obtained by microscopic counting on diaphragm
The Hela cell theoretical values being added in cell quantity divided by blood sample obtain the rate of recovery, then the rate of recovery of this experiment HeLa cell is 94%.
Claims (4)
1. a kind of detaching and be enriched with peripheral blood rare cell using tunnel magnetic field technology（Fetal nucleated red blood, circulating tumor are thin
Born of the same parents etc.) method, it is characterised in that include the following steps:
（a）Rare cell will be coated with（Fetal nucleated red blood, circulating tumor cell etc.) specific antibody or aptamer
Magnetic bead is added in human peripheral blood and is incubated, and the rare cell in peripheral blood is made to be combined with magnetic bead;
（b）The processed blood sample of magnetic bead is flowed through into the film tube in tunnel magnetic field, under magnetic fields, carries different number
The cellular retention of magnetic bead is detached in the different location of thin film tube wall to realize to sort;
（c）Cleaning solution is inputted to film tube, removal does not carry the cell of magnetic bead;
（d）Applying negative pressure to film tube makes film tube shrink to form diaphragm, sealing both ends, fixes and preserves thin after detaching
Born of the same parents, to carry out subsequently identifying parting.
2. tunnel magnetic field technology separation according to claim 1 and enrichment peripheral blood rare cell（Fetal nucleated red blood,
Circulating tumor cell etc.) method, which is characterized in that this method includes but not limited to, before applying negative pressure to film tube, and
Magnetic field keeps the lower dying operation for implementing cell（It is required according to colouring method, sequentially inputs such as reaction solution to film tube, consolidates
Determine liquid, dyeing liquor, cleaning solution etc., reaches dyeing purpose）, so that the cell to separation makees Morphological Identification.
3. tunnel magnetic field technology separation according to claim 1 and enrichment peripheral blood rare cell（Fetal nucleated red blood,
Circulating tumor cell etc.) method, which is characterized in that this method includes but not limited to before blood sample loading, to use isotonic solution
Body forms a lasting mobile phase in film tube, in favor of weak thin of magnetic induction after the separation process of cell and end of the sample
Born of the same parents are washed out.
4. tunnel magnetic field technology separation according to claim 1 and enrichment peripheral blood rare cell（Fetal nucleated red blood,
Circulating tumor cell etc.) method, which is characterized in that the film tube used in this method be include but not limited to that transparent plastics are thin
Membrane tube.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628402A (en) * | 2019-02-13 | 2019-04-16 | 妙顺（上海）生物科技有限公司 | A kind of primary organoid cultural method of gastric cancer tumor |
| CN112662613A (en) * | 2019-10-15 | 2021-04-16 | 深圳市红莓生物科技有限公司 | Magnetic cell separation method |
| CN115109780A (en) * | 2022-06-22 | 2022-09-27 | 湖南大学 | Aptamer capable of specifically recognizing and combining with circulating fetal nucleated red blood cells, complementary sequence and application of aptamer and complementary sequence |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643701A (en) * | 2009-07-23 | 2010-02-10 | 清华大学 | Cell sorter micro-fluidic chip based on immunomagnetic separation technology and application thereof in aspect of enrichment of rare cells |
| CN102527353A (en) * | 2011-12-21 | 2012-07-04 | 深圳市老年医学研究所 | Preparation for nanometer magnetic immunomicrosphere for enriching circulating cancer cells |
| CN102690786A (en) * | 2012-06-05 | 2012-09-26 | 武汉格蓝丽富科技有限公司 | Cell enriching, separating and extracting method and instrument and single cell analysis method |
| CN102762712A (en) * | 2010-01-21 | 2012-10-31 | 百赛普有限公司 | Magnetic separation of rare cells |
| CN102911864A (en) * | 2012-10-15 | 2013-02-06 | 宁波美晶医疗技术有限公司 | Separator for rare cells in blood |
| CN203144416U (en) * | 2013-02-25 | 2013-08-21 | 宁波美晶医疗技术有限公司 | Device for separating rare cells in blood |
| CN103630440A (en) * | 2013-11-28 | 2014-03-12 | 武汉大学 | Enriching method of circulating tumor cells |
| CN107356744A (en) * | 2017-07-13 | 2017-11-17 | 珠海丽珠圣美医疗诊断技术有限公司 | A kind of method and its kit of circulating tumor cell sorting and/or enrichment |
-
2018
- 2018-04-21 CN CN201810362931.4A patent/CN108546676A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643701A (en) * | 2009-07-23 | 2010-02-10 | 清华大学 | Cell sorter micro-fluidic chip based on immunomagnetic separation technology and application thereof in aspect of enrichment of rare cells |
| CN102762712A (en) * | 2010-01-21 | 2012-10-31 | 百赛普有限公司 | Magnetic separation of rare cells |
| CN102527353A (en) * | 2011-12-21 | 2012-07-04 | 深圳市老年医学研究所 | Preparation for nanometer magnetic immunomicrosphere for enriching circulating cancer cells |
| CN102690786A (en) * | 2012-06-05 | 2012-09-26 | 武汉格蓝丽富科技有限公司 | Cell enriching, separating and extracting method and instrument and single cell analysis method |
| CN102911864A (en) * | 2012-10-15 | 2013-02-06 | 宁波美晶医疗技术有限公司 | Separator for rare cells in blood |
| CN203144416U (en) * | 2013-02-25 | 2013-08-21 | 宁波美晶医疗技术有限公司 | Device for separating rare cells in blood |
| CN103630440A (en) * | 2013-11-28 | 2014-03-12 | 武汉大学 | Enriching method of circulating tumor cells |
| CN107356744A (en) * | 2017-07-13 | 2017-11-17 | 珠海丽珠圣美医疗诊断技术有限公司 | A kind of method and its kit of circulating tumor cell sorting and/or enrichment |
Non-Patent Citations (3)
| Title |
|---|
| LU NING-NING ET AL.: "Biotin-Triggered Decomposable Immunomagnetic Beads for Capture and Release of Circulating Tumor Cells", 《ACS APPLIED MATERIALS & INTERFACES》 * |
| 李福来 等: "基于免疫磁分离的微流控技术在循环肿瘤细胞富集分离中应用的研究进展", 《中华检验医学杂志》 * |
| 杜鹃: "《医学细胞与分子生物学理论与技术》", 31 July 2012, 吉林大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628402A (en) * | 2019-02-13 | 2019-04-16 | 妙顺（上海）生物科技有限公司 | A kind of primary organoid cultural method of gastric cancer tumor |
| CN112662613A (en) * | 2019-10-15 | 2021-04-16 | 深圳市红莓生物科技有限公司 | Magnetic cell separation method |
| CN115109780A (en) * | 2022-06-22 | 2022-09-27 | 湖南大学 | Aptamer capable of specifically recognizing and combining with circulating fetal nucleated red blood cells, complementary sequence and application of aptamer and complementary sequence |
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