CN103255054B - Slide block closed type biological chip - Google Patents
Slide block closed type biological chip Download PDFInfo
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- CN103255054B CN103255054B CN201310145215.8A CN201310145215A CN103255054B CN 103255054 B CN103255054 B CN 103255054B CN 201310145215 A CN201310145215 A CN 201310145215A CN 103255054 B CN103255054 B CN 103255054B
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Abstract
The invention discloses a slide block closed type biological chip. The slide block closed type biological chip comprises a housing, a slice and a glass slide, wherein the slice and the glass slide are arranged in the housing; the housing comprises a first slide block and a second slide block, wherein the first slide block and the second slide block are synthesized to form an inner cavity for placing the slice and the glass slide; a locking mechanism is arranged between the first slide block and the second slide block; under a locking state, the locking mechanism is used for fixing the slice and the glass slide to the inner cavity formed by the first slide block and the second slide block; when the locking mechanism is under an open state, the first slide block and the second slide block can be separated from each other, so that the glass slide can be taken out. The slide block type closed biological chip disclosed by the invention is relatively simple in structure and very convenient to operate.
Description
Technical field
The present invention relates to a kind of blood testing biochip, especially relate to a kind of slide block closed type biochip.
Background technology
Blood testing biochip is the diagnostic tool of future generation that detects rare cell in the blood relevant to major disease, and rare cell comprises cell with specified protein mark of circulating tumor cell (CTC), pernicious stem cell and the pathology of circulation in blood sample etc.Relevant studies confirm that, the circulating tumor cell quantity occurring in patient's blood sample and patient's early diagnosis and survival rate have very strong dependency.Therefore, the rare cell determination and analysis of the blood sample to patient is the key that improves tumor disease early discovery rate and personalized treatment.
Because the CTCs quantity of patient blood sample is very little, CTC is detected and there is very large challenge.Flow cytometer detection method is a kind of CTC detection method the most generally using, and CTC is larger than general cell space, and caryoplasm ratio is high, endochylema no particulate matter is few, expression specificity antigen, its characteristic to the identification of scattering of light and immunofluorescence of flow cytometry is separated CTC from total cell.Current up-to-date flow cytometer BD FACSAria is the new breakthrough of streaming high speed cell sorting, but its defect that is difficult to overcome is: instantaneous laser hits will bring damage to cell, sorting poor activity; Due to the intersection existing between spectrum, easily sneak into false-positive cell; The required compensation of fluorescent signal is difficult to allotment; Tend to the phenomenon that occurs that cytoadherence is agglomerating, the obstruction of efferent tract happens occasionally; The filtration cell of having no progeny in sorting is again prepared sample, and this has increased the probability of polluting, and cytoactive is greatly impaired.Still deposit dispute with the method for the CTC in Flow cytometry tumour patient merely; Form fractionation method in addition, from white corpuscle, the quantity of isolated CTCs and density are smaller, can stay simultaneously a large amount of similar and be not enough to be considered as the cell of CTC with CTC form, as the acyclic tumour cell equally little with white corpuscle.Also have immunologic detection method, because CTCs has the high special effect property that separates, can utilize specific marker, target CTC is carried out to mark, but need the extra immunofluorescence dyeing screening step that increases.
So, be necessary to provide a kind of simple in structure, easy to operate blood testing biochip from patient blood sample, to separate the specific protein cell plastid of rare cell and/or pathology.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of simple in structure, easy to operate for screening the slide block closed type biochip of blood rare cell.
The present invention solves the problems of the technologies described above adopted technical scheme:
A kind of slide block closed type biochip, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in described housing, described housing comprises the first slide block and the second slide block, after the first slide block and the second slide block are synthetic, form one for placing described thin slice and the inner chamber of slide glass, between the first described slide block and the second slide block, be provided with lockout mechanism, under lockout mechanism lock-out state, thin slice and slide glass are fixed in the inner chamber of the first slide block and the formation of the second slide block, under lockout mechanism open mode, the first slide block and the second slide block can be separated from each other, slide glass can be removed.Housing plays the effect of protection thin slice and slide glass; closing up and locking with lockout mechanism by two slide blocks; thin slice and slide glass are fitted tightly and interfixed; form airtight microfluidic channel; after lockout mechanism is opened; two slide blocks separate, and slide glass can take out in housing, for subsequent analysis processing.
Described thin slice one side is provided with groove, and the groove on thin slice is close to towards slide glass and by thin slice on described slide glass, makes to form between thin slice and slide glass an airtight microfluidic channel; On described thin slice, offer the fluid hole and the inlet opening that are communicated with described microfluidic channel.
Described inner chamber is positioned at the centre of housing, and lockout mechanism comprises two locked components, is respectively the first locked component and the second locked component, and the first locked component and the second locked component are symmetrically distributed in the both sides of inner chamber.
On the first described slide block, be provided with first hook of extending to the second slide block, on the second described slide block, be provided with the second hook matching with the first hook, in the time that the first hook and the second hook are hooked mutually, the first slide block and the second slide block interfix, in the time that the first hook separates with the second hook, the first slide block and the second slide block are separated from each other.
The first described hook comprises extension rod and the first hook, the second hook comprises the second hook, the first hook is made up of the first hypotenuse and the first straight flange, the second hook is made up of the second hypotenuse and the second straight flange, the first described straight flange is vertical with the bearing of trend of extension rod with the residing plane of the second straight flange, together with the first straight flange touches with the second straight flange time, the first hook and the second hook hook mutually.
The central point of the first described hypotenuse is than the central point of the first straight flange further from the first slide block, the central point of the second described hypotenuse is than more close first slide block of central point of the second straight flange, in the time that the first slide block is pushed the second slide block to, the first hypotenuse and the second hypotenuse mutually push and make extension rod oblique to a lateral bending, together with the first straight flange touches with the second straight flange time, extension rod returns to its straight state.
The outside of the second described slide block is connected with by flexible link the portion of starting, the second described hook is arranged on the inner side of the portion of starting, in the time starting portion in horizontality, the first hook and the second hook hook mutually, the portion of starting is positioned at the top of the second hook, the portion that will start is taking flexible link when basic point starts, and the first hook and the second hook are thrown off, and the first slide block and the second slide block can be separated from each other.
Between the first described slide block and the second slide block, be provided with positioning component, described positioning component comprises the pilot hole and the locating dowel that cooperatively interact, and described pilot hole is arranged on the inner side of the second slide block, and described locating dowel is arranged on the inner side of the first slide block.
Inner chamber comprises the upper inner chamber and the lower inner cavity that are interconnected, and described thin slice is arranged in inner chamber, and described slide glass is arranged in lower inner cavity.
Below described housing, offer the empty avoiding window that can embed magnetic substance, described empty avoiding window be positioned at microfluidic channel under, above described housing, be provided with viewing window, on described fluid hole, be connected with the first pipe joint element, on described inlet opening, be connected with the second pipe joint element, the first described pipe joint element and the second pipe joint element are bonded on described thin slice.The magnet that is used to form gradient magnetic is placed on the below of slide glass, and offering of empty avoiding window can avoid housing to stop magnetic field, make magnetic field more useful effect in the blood sample of the microfluidic channel of flowing through.Blood. reservoir is communicated with the first pipe joint element by pipe connecting, the second pipe joint element is communicated with liquor pump by pipe connecting, under the suction of liquor pump, blood sample in storer is in the first pipe joint element flows into microfluidic channel, blood sample in microfluidic channel flows to liquor pump through the second pipe joint element, and blood sample is with a certain suitable speed runoff microfluidic channel.
Compared with prior art, advantage of the present invention is first in blood sample, to put into magnetic nano-particle, magnetic nano-particle surface is with antibody, be adsorbed on target cell by immune response, then blood sample rare cell being labeled the microfluidic channel of flowing through, the rare cell that is attached with magnetic nano-particle in blood sample is placed on the gradient magnetic guiding that the magnetic substance below biochip sends, and then is collected on slide glass.Housing plays the effect of protection thin slice and slide glass; closing up and locking with lockout mechanism by two slide blocks; thin slice and slide glass are fitted tightly and interfixed; form airtight microfluidic channel; after lockout mechanism is opened; two slide blocks separate, due between thin slice and slide glass for removably connecting, slide glass can take out in housing.Rare cell on slide glass is carried out to subsequent analysis.The present invention is relatively simple for structure, operates very convenient.
Brief description of the drawings
Fig. 1 is exploded view of the present invention (one);
Fig. 2 is the structure iron of thin slice of the present invention;
Fig. 3 is exploded view of the present invention (two);
Fig. 4 is the state graph that the first slide block of the present invention pushes away nearly the second slide block;
Fig. 5 is the state graph () of the portion of starting of the present invention while starting;
Fig. 6 is the state graph (two) of the portion of starting of the present invention while starting;
Fig. 7 is the structure iron of the portion of starting of the present invention while starting;
Fig. 8 is stereographic map of the present invention (one);
Fig. 9 is stereographic map of the present invention (two);
Figure 10 is sectional view of the present invention.
Embodiment
Below in conjunction with accompanying drawing, embodiment is described in further detail the present invention.
A kind of slide block closed type biochip, comprise housing 1, thin slice 2 and slide glass 3, thin slice 2 and slide glass 3 are arranged in housing 1, housing 1 comprises the first slide block 4 and the second slide block 5, after the first slide block 4 and the second slide block 5 are synthetic, form an inner chamber for placing sheets 2 and slide glass 3, between the first slide block 4 and the second slide block 5, be provided with lockout mechanism, under lockout mechanism lock-out state, thin slice 2 and slide glass 3 are fixed in the inner chamber 6 that the first slide block 4 and the second slide block 4 form, under lockout mechanism open mode, the first slide block 4 and the second slide block 5 can be separated from each other, slide glass 3 can be removed.
Thin slice 2 one sides are provided with groove 7, and the groove 7 on thin slice 2 is close on slide glass 3 towards slide glass 3 and by thin slice 2, make to form between thin slice 2 and slide glass 3 an airtight microfluidic channel; On thin slice 2, offer the fluid hole and the inlet opening that are communicated with microfluidic channel.
Inner chamber 6 is positioned at the centre of housing 1, and lockout mechanism comprises two locked components, is respectively the first locked component 8 and the second locked component, and the first locked component 8 and the second locked component are symmetrically distributed in the both sides of inner chamber 6.
On the first slide block 4, be provided with first hook 9 of extending to the second slide block 5, on the second slide block 5, be provided with the second hook 10 matching with the first hook 9, in the time that the first hook 9 and the second hook 10 are hooked mutually, the first slide block 4 and the second slide block 5 interfix, in the time that the first hook 9 separates with the second hook 10, the first slide block 4 and the second slide block 5 are separated from each other.
The first hook 9 comprises extension rod 11 and the first hook 12, the second hook 10 comprises the second hook 13, the first hook 12 is made up of the first hypotenuse 14 and the first straight flange 15, the second hook 13 is made up of the second hypotenuse 16 and the second straight flange 17, the first straight flange 15 is vertical with the bearing of trend of extension rod 11 with the residing plane of the second straight flange 17, together with the first straight flange 15 touches with the second straight flange 17 time, the first hook 12 and the second hook 13 hook mutually.
The central point of the first hypotenuse 14 is than the central point of the first straight flange 15 further from the first slide block 4, the central point of the second hypotenuse 16 is than more close first slide block 4 of central point of the second straight flange 17, in the time that the first slide block 4 is pushed the second slide block 5 to, the first hypotenuse 14 and the second hypotenuse 16 mutually extruding make extension rod 11 oblique to a lateral bending, together with the first straight flange 15 touches with the second straight flange 17 time, extension rod 11 returns to its straight state.
The outside of the second slide block 5 is connected with the portion of starting 19 by flexible link 18, the second hook 13 is arranged on the inner side of the portion of starting 19, in the time starting portion 19 in horizontality, the first hook 12 and the second hook 13 hook mutually, start the top that portion 19 is positioned at the second hook 13, to start portion 19 taking flexible link 18 when basic point starts, the first hook 12 and the second hook 13 are thrown off, the first slide block 4 and the second slide block 5 can be separated from each other.
Between the first slide block 4 and the second slide block 5, be provided with positioning component, positioning component comprises the pilot hole 20 and the locating dowel 21 that cooperatively interact, and pilot hole 20 is arranged on 5 inner sides of the second slide block, and locating dowel 21 is arranged on the inner side of the first slide block 4.
Inner chamber 6 comprises the upper inner chamber 61 and the lower inner cavity 62 that are interconnected, and thin slice 2 is arranged in inner chamber 61, and slide glass 3 is arranged in lower inner cavity 62.
Below housing 1, offer the empty avoiding window 22 that can embed magnetic substance, empty avoiding window 22 be positioned at microfluidic channel under, above housing 1, be provided with viewing window 23, on fluid hole, be connected with the first pipe joint element 24, on inlet opening, being connected with the second pipe joint element 25, the first pipe joint elements 24 and the second pipe joint element 25 is bonded on thin slice.
The tackiness agent that pipe joint element and thin slice 2 adopt, requirement is to produce any impact to blood sample, ensures the stopping property of system simultaneously, prevents blood sample leakage.The thickness of thin slice 2 is 8mm or 10mm or 12mm, and the degree of depth of groove 11 is 0.4mm or 0.5mm or 0.6mm, and the diameter of bore of inlet opening and fluid hole is 1.5mm or 1.58mm or 1.7mm.
Claims (2)
1. a slide block closed type biochip, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in described housing, it is characterized in that described housing comprises the first slide block and the second slide block, after the first slide block and the second slide block are synthetic, form one for placing described thin slice and the inner chamber of slide glass, between the first described slide block and the second slide block, be provided with lockout mechanism, under lockout mechanism lock-out state, thin slice and slide glass are fixed in the inner chamber of the first slide block and the formation of the second slide block, under lockout mechanism open mode, the first slide block and the second slide block can be separated from each other, slide glass can be removed,
Described thin slice one side is provided with groove, and the groove on thin slice is close to towards slide glass and by thin slice on described slide glass, makes to form between thin slice and slide glass an airtight microfluidic channel; On described thin slice, offer the fluid hole and the inlet opening that are communicated with described microfluidic channel;
Described inner chamber is positioned at the centre of housing, and lockout mechanism comprises two locked components, is respectively the first locked component and the second locked component, and the first locked component and the second locked component are symmetrically distributed in the both sides of inner chamber;
On the first described slide block, be provided with first hook of extending to the second slide block, on the second described slide block, be provided with the second hook matching with the first hook, in the time that the first hook and the second hook are hooked mutually, the first slide block and the second slide block interfix, in the time that the first hook separates with the second hook, the first slide block and the second slide block are separated from each other;
The first described hook comprises extension rod and the first hook, the second hook comprises the second hook, the first hook is made up of the first hypotenuse and the first straight flange, the second hook is made up of the second hypotenuse and the second straight flange, the first described straight flange is vertical with the bearing of trend of extension rod with the residing plane of the second straight flange, together with the first straight flange touches with the second straight flange time, the first hook and the second hook hook mutually;
The central point of the first described hypotenuse is than the central point of the first straight flange further from the first slide block, the central point of the second described hypotenuse is than more close first slide block of central point of the second straight flange, in the time that the first slide block is pushed the second slide block to, the first hypotenuse and the second hypotenuse mutually push and make extension rod oblique to a lateral bending, together with the first straight flange touches with the second straight flange time, extension rod returns to its straight state;
The outside of the second described slide block is connected with by flexible link the portion of starting, the second described hook is arranged on the inner side of the portion of starting, in the time starting portion in horizontality, the first hook and the second hook hook mutually, the portion of starting is positioned at the top of the second hook, the portion that will start is taking flexible link when basic point starts, and the first hook and the second hook are thrown off, and the first slide block and the second slide block can be separated from each other;
Between the first described slide block and the second slide block, be provided with positioning component, described positioning component comprises the pilot hole and the locating dowel that cooperatively interact, and described pilot hole is arranged on the inner side of the second slide block, and described locating dowel is arranged on the inner side of the first slide block;
Inner chamber comprises the upper inner chamber and the lower inner cavity that are interconnected, and described thin slice is arranged in inner chamber, and described slide glass is arranged in lower inner cavity.
2. a kind of slide block closed type biochip according to claim 1, it is characterized in that offering the empty avoiding window that can embed magnetic substance below described housing, described empty avoiding window be positioned at microfluidic channel under, above described housing, be provided with viewing window, on described fluid hole, be connected with the first pipe joint element, on described inlet opening, be connected with the second pipe joint element, the first described pipe joint element and the second pipe joint element are bonded on described thin slice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310145215.8A CN103255054B (en) | 2013-04-24 | 2013-04-24 | Slide block closed type biological chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310145215.8A CN103255054B (en) | 2013-04-24 | 2013-04-24 | Slide block closed type biological chip |
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| CN103255054A CN103255054A (en) | 2013-08-21 |
| CN103255054B true CN103255054B (en) | 2014-08-06 |
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| CN201310145215.8A Active CN103255054B (en) | 2013-04-24 | 2013-04-24 | Slide block closed type biological chip |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN209872930U (en) * | 2019-05-06 | 2019-12-31 | 北京京东方技术开发有限公司 | Experimental device for be used for biochip |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0201780A1 (en) * | 1985-04-27 | 1986-11-20 | Jan Hastka | Apparatus and method for cytological and histological examinations of sample slides |
| CN2340201Y (en) * | 1998-09-01 | 1999-09-29 | 吴云晓 | Match box with drawing cover |
| CN2466765Y (en) * | 2001-01-19 | 2001-12-19 | 刘忠泉 | Disc cassette |
| KR200443800Y1 (en) * | 2007-09-10 | 2009-03-16 | 한국기술산업 (주) | Fixing apparatus for fixing a bio-chip in analyzing device |
| CN101576523A (en) * | 2009-06-11 | 2009-11-11 | 上海交通大学 | Method for detecting tumour cells by adopting microelectrode array impedance biosensor chip |
| CN102732415A (en) * | 2012-04-24 | 2012-10-17 | 武汉介观生物科技有限责任公司 | High-efficiency rare-cell-capturing integrated chip, manufacturing method thereof, and application thereof |
| CN202693392U (en) * | 2012-07-17 | 2013-01-23 | 上海聚阵生物科技有限公司 | Biochip spotting instrument |
| CN102965276A (en) * | 2012-11-20 | 2013-03-13 | 宁波美晶医疗技术有限公司 | Biochip for screening rare cells in blood |
| CN203238270U (en) * | 2013-04-24 | 2013-10-16 | 宁波美晶医疗技术有限公司 | Slider closed type biochip |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101135084B1 (en) * | 2008-12-23 | 2012-04-16 | 한국전자통신연구원 | Microfluidic device and microfluidic analysis equipment |
-
2013
- 2013-04-24 CN CN201310145215.8A patent/CN103255054B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0201780A1 (en) * | 1985-04-27 | 1986-11-20 | Jan Hastka | Apparatus and method for cytological and histological examinations of sample slides |
| CN2340201Y (en) * | 1998-09-01 | 1999-09-29 | 吴云晓 | Match box with drawing cover |
| CN2466765Y (en) * | 2001-01-19 | 2001-12-19 | 刘忠泉 | Disc cassette |
| KR200443800Y1 (en) * | 2007-09-10 | 2009-03-16 | 한국기술산업 (주) | Fixing apparatus for fixing a bio-chip in analyzing device |
| CN101576523A (en) * | 2009-06-11 | 2009-11-11 | 上海交通大学 | Method for detecting tumour cells by adopting microelectrode array impedance biosensor chip |
| CN102732415A (en) * | 2012-04-24 | 2012-10-17 | 武汉介观生物科技有限责任公司 | High-efficiency rare-cell-capturing integrated chip, manufacturing method thereof, and application thereof |
| CN202693392U (en) * | 2012-07-17 | 2013-01-23 | 上海聚阵生物科技有限公司 | Biochip spotting instrument |
| CN102965276A (en) * | 2012-11-20 | 2013-03-13 | 宁波美晶医疗技术有限公司 | Biochip for screening rare cells in blood |
| CN203238270U (en) * | 2013-04-24 | 2013-10-16 | 宁波美晶医疗技术有限公司 | Slider closed type biochip |
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| CN103255054A (en) | 2013-08-21 |
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