CN103255055A - Buckle type biological chip - Google Patents
Buckle type biological chip Download PDFInfo
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- CN103255055A CN103255055A CN201310145525XA CN201310145525A CN103255055A CN 103255055 A CN103255055 A CN 103255055A CN 201310145525X A CN201310145525X A CN 201310145525XA CN 201310145525 A CN201310145525 A CN 201310145525A CN 103255055 A CN103255055 A CN 103255055A
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- enclosing cover
- slide glass
- thin slice
- snap
- clamping block
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Abstract
The invention discloses a buckle type biological chip. The biological chip comprises a shell, a slice and a glass slide, wherein the slice and the glass slide are arranged in the shell; one side of an upper outer cover and one side of a lower outer cover are connected with each other by a soft connecting piece; a fixed buckle with a slot is arranged on the other side of the upper outer cover; a locking assembly is arranged on the other side of the lower outer cover and comprises a rear and a locking block; the rear is connected with the lower outer cover; the locking block is arranged in the front; and an easily broken part is arranged on the rear of the locking assembly. The biological chip has the advantages that the easily broken part is cut off with scissors, thus separating the locking block from the lower outer cover; one sides of the upper and lower outer covers can be separated, so that the glass slide can be taken out of the shell, and subsequent analysis is carried out on the rare cells on the glass slide; and the biological chip has a simpler structure and is very convenient to operate.
Description
Technical field
The present invention relates to a kind of blood testing biochip, especially relate to a kind of snap-type biochip.
Background technology
The blood testing biochip is the diagnostic tool of future generation that detects rare cell in the blood relevant with major disease, and rare cell comprises cell with specified protein mark of circulating tumor cell (CTC), pernicious stem cell and the pathology of circulation in blood sample etc.Relevant studies confirm that the circulating tumor cell quantity that occurs in the patients'blood sample and patient's early diagnosis and survival rate have very strong dependency.Therefore, rare cell detection and the analysis to the patients'blood sample is the key that improves tumor disease early discovery rate and personalized treatment.
Because the CTCs quantity of patient blood sample is very little, CTC is detected have very big challenge.The flow cytometer detection method is a kind of CTC detection method of the most generally using, and CTC is bigger than general cell space, caryoplasm ratio height, the endochylema no particulate matter is few, expression specificity antigen, its characteristic to scattering of light and immunofluorescence identification of flow cytometry is separated CTC from total cell.Present up-to-date flow cytometer BD FACSAria is the new breakthrough of streaming high speed cell sorting, but its defective that is difficult to overcome is: instantaneous laser hits will bring damage to cell, the sorting poor activity; Because the intersection that exists between spectrum is easily sneaked into false-positive cell; The required compensation of fluorescent signal is difficult to allotment; Tend to occur the agglomerating phenomenon of cytoadherence, the obstruction of efferent tract happens occasionally; The filtration cell of having no progeny in the sorting again prepares sample, and this has increased the probability of polluting, and cytoactive is impaired greatly.Still deposit dispute with the method for the CTC in the Flow cytometry tumour patient merely; Form separation method in addition, the quantity of isolated CTCs and density are smaller from white corpuscle, can stay simultaneously a large amount of similar and be not enough to be considered as the cell of CTC with the CTC form, as the same little acyclic tumour cell with white corpuscle.Also have immunologic detection method, because CTCs has the high special effect property of separating, can utilize the specific markers thing, target CTC is carried out mark, but need the extra immunofluorescence dyeing screening step that increases.
So, be necessary to provide a kind of simple in structure, easy to operate blood testing biochip from the patient blood sample, to separate the specific protein cell plastid of rare cell and/or pathology.
Summary of the invention
Technical problem to be solved by this invention provides a kind of simple in structure, easy to operate snap-type biochip that is used for screening blood rare cell.
The present invention solves the problems of the technologies described above the technical scheme that adopts: a kind of snap-type biochip, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in the described housing, described housing comprises enclosing cover and following enclosing cover, the described medial surface of going up enclosing cover is pressed on the upper surface of thin slice, the described medial surface of enclosing cover down is pressed on the lower surface of slide glass, a described side and a described side of enclosing cover down that goes up enclosing cover interconnects by flexible link, the described opposite side of going up enclosing cover is provided with the clip with slot, the described opposite side of enclosing cover down is provided with locked component, described locked component comprises the rear portion that is connected with following enclosing cover and is positioned at anterior clamping block, the rear portion of described locked component is provided with easy breaking off section, described clamping block is inserted in the described slot, and last enclosing cover and following enclosing cover are fixed with each other; The external force effect causes the easy breaking off section fracture, and clamping block covers outside down and separates, and clamping block is stayed in the slot of clip, and the opposite side of last enclosing cover is separated from each other with the opposite side of following enclosing cover, takes out slide glass.
Described locked component is made of plastics, and is provided with the first tooth limit in the described slot, and described clamping block includes the second tooth limit, and the first tooth limit and the second tooth limit cooperatively interact clamping block is fixed in the described slot.
The described first tooth limit comprises first plane and first inclined-plane, the described second tooth limit comprises second plane and second inclined-plane, described first plane is vertical with the direction of insertion of clamping block with second plane, and second plane on first plane on the first tooth limit and the second tooth limit cooperatively interacts just that clamping block is fixed in the described slot.Clamping block is inserted in the slot process, first inclined-plane and the second inclined-plane mutual extrusion after strain, first plane and second plane interact, and clamping block can't be extracted in slot.
The thickness of described easy breaking off section is less than the thickness of locked component.Use scissors that easy breaking off section is cut off, thereby clamping block is separated with following enclosing cover, last enclosing cover can separate with following enclosing cover, and clamping block is stayed in the clip of enclosing cover.
Described thin slice one side is provided with groove, and the groove on the thin slice is close on the described slide glass towards slide glass and with thin slice, makes and forms an airtight microfluidic channel between thin slice and the slide glass; Offer the fluid hole and the inlet opening that are communicated with described microfluidic channel on the described thin slice.
Be provided with in the described upward enclosing cover and hold frame, described thin slice is arranged on described holding in the frame.
Be close to that face of slide glass on the thin slice and be provided with first sealed strip, described first sealed strip is positioned at the upper edge of groove, and first sealed strip and slide glass closely cooperate, and the medial surface of following enclosing cover is provided with second sealed strip, and second sealed strip and slide glass closely cooperate.The setting of first sealing-ring forms airtight microfluidic channel, avoids blood leakage.Housing pushes slide glass by second sealed strip, can prevent that slide glass from breaking because of extruding simultaneously.
Described down enclosing cover offers the empty avoiding window that can embed magnetic substance, described empty avoiding window be positioned at microfluidic channel under.The magnet that is used to form gradient magnetic is placed on the below of slide glass, and offering of empty avoiding window can avoid housing to stop magnetic field, make magnetic field more useful effect in the blood sample of the microfluidic channel of flowing through.
Be connected with first pipe joint element on the described fluid hole, be connected with second pipe joint element on the described inlet opening, described first pipe joint element and second pipe joint element are bonded on the described thin slice.Blood. reservoir is communicated with first pipe joint element by pipe connecting, second pipe joint element is communicated with liquor pump by pipe connecting, under the suction of liquor pump, blood sample in the storer is in first pipe joint element flows into microfluidic channel, blood sample in the microfluidic channel flows to liquor pump through second pipe joint element, and blood sample is with a certain proper speed runoff microfluidic channel.
Following enclosing cover comprises that bottom and following four side, an end of following enclosing cover are provided with the small gap that convenient finger fetches slide glass, and this small gap one is distributed on the first side on bottom and the following four side.
Compared with prior art, advantage of the present invention is to put into earlier magnetic nano-particle in blood sample, the magnetic nano particle sub-surface has antibody, be adsorbed on the target cell by immune response, the blood sample that rare cell has been labeled the microfluidic channel of flowing through then, the rare cell that is attached with magnetic nano-particle in the blood sample is placed on the gradient magnetic guiding that the magnetic substance below the biochip sends, and then be collected on the slide glass, since between thin slice and the slide glass for removably connecting, use scissors that easy breaking off section is cut off, thereby clamping block is separated with following enclosing cover, last enclosing cover can one-sidedly separate with following enclosing cover, slide glass can take out in housing, and the rare cell on the slide glass is carried out subsequent analysis.The present invention is relatively simple for structure, and it is very convenient to operate.
Description of drawings
Fig. 1 is shape assumption diagram of the present invention;
Fig. 2 is cut-away view of the present invention (one);
Fig. 3 is exploded view of the present invention (one);
Fig. 4 is cut-away view of the present invention (two);
Fig. 5 is exploded view of the present invention (two);
Fig. 6 is the exploded view after easy breaking off section of the present invention is cut off;
Fig. 7 is sectional view of the present invention.
Embodiment
Describe in further detail below in conjunction with the present invention of accompanying drawing embodiment.
A kind of snap-type biochip, comprise housing 1, thin slice 2 and slide glass 3, thin slice 1 and slide glass 3 are arranged in the housing 1, housing 1 comprises enclosing cover 4 and following enclosing cover 5, the medial surface of last enclosing cover 4 is pressed on the upper surface of thin slice 1, the medial surface of following enclosing cover 5 is pressed on the lower surface of slide glass 3, one side of last enclosing cover 4 interconnects by flexible link 6 with a side of following enclosing cover 5, the opposite side of last enclosing cover 4 is provided with the clip 8 with slot 7, the opposite side of following enclosing cover 5 is provided with locked component, locked component comprises the rear portion 9 that is connected with following enclosing cover 5 and is positioned at anterior clamping block 10, the rear portion 9 of locked component is provided with easy breaking off section 11, clamping block 10 is inserted in the slot 7, and last enclosing cover 4 and following enclosing cover 5 are fixed with each other; The external force effect causes easy breaking off section 11 fractures, and clamping block 10 is separated from following enclosing cover 5, and clamping block 10 is stayed in the slot 7 of clip 8, and the opposite side of last enclosing cover 4 is separated from each other with the opposite side of following enclosing cover 5, takes out slide glass 3.
Locked component is made of plastics, and is provided with the first tooth limit 12 in the slot 7, and clamping block 10 includes 13, the first tooth limits 12, the second tooth limit and the second tooth limit 13 and cooperatively interacts clamping block 10 is fixed in the slot 7.
The first tooth limit 12 comprises first plane 14 and first inclined-plane 15, the second tooth limit 13 comprises second plane 16 and second inclined-plane 17, first plane 14 is vertical with the direction of insertion of clamping block 10 with second plane 16, and first plane 14 on the first tooth limit 12 and second plane 16 on the second tooth limit 13 cooperatively interact just that clamping block 10 is fixed in the slot 7.
The thickness of easy breaking off section 11 is less than the thickness at locked component rear portion 9.
Be provided with in the last enclosing cover 4 and hold frame 23, thin slice 2 is arranged on described holding in the frame 23.
Be close to slide glass 3 those faces on the thin slice 2 and be provided with first sealed strip 18, first sealed strip 18 is positioned at the upper edge of groove 17, first sealed strip 18 closely cooperates with slide glass 3, and the medial surface of following enclosing cover 5 is provided with second sealed strip, 19, the second sealed strips 19 and closely cooperates with slide glass 3.
Following enclosing cover 5 offers the empty avoiding window 20 that can embed magnetic substance, empty avoiding window 20 be positioned at microfluidic channel under.
Be connected with first pipe joint element 21 on the fluid hole, be connected with second pipe joint element, 22, the first pipe joint elements 21 and second pipe joint element 22 on the inlet opening and be bonded on the thin slice 2.
Following enclosing cover 5 comprises bottom 51 and following four side, and an end of following enclosing cover 5 is provided with the small gap 52 that convenient finger fetches slide glass, and these small gap 52 one are distributed on the first side 53 on bottom 51 and the following four side.
The tackiness agent that pipe joint element and thin slice 2 adopt, requirement is to produce any influence to blood sample, guarantees the stopping property of system simultaneously, prevents the blood sample leakage.The thickness of thin slice 2 is 8mm or 10mm or 12mm, and the degree of depth of groove 17 is 0.4mm or 0.5mm or 0.6mm, and the diameter of bore of inlet opening and fluid hole is 1.5mm or 1.58mm or 1.7mm.
Claims (10)
1. snap-type biochip, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in the described housing, described housing comprises enclosing cover and following enclosing cover, the described medial surface of going up enclosing cover is pressed on the upper surface of thin slice, the described medial surface of enclosing cover down is pressed on the lower surface of slide glass, a described side and a described side of enclosing cover down that goes up enclosing cover interconnects by flexible link, it is characterized in that the described opposite side of going up enclosing cover is provided with the clip with slot, the described opposite side of enclosing cover down is provided with locked component, described locked component comprises the rear portion that is connected with following enclosing cover and is positioned at anterior clamping block, the rear portion of described locked component is provided with easy breaking off section, described clamping block is inserted in the described slot, and last enclosing cover and following enclosing cover are fixed with each other; The external force effect causes the easy breaking off section fracture, and clamping block covers outside down and separates, and clamping block is stayed in the slot of clip, and the opposite side of last enclosing cover is separated from each other with the opposite side of following enclosing cover, takes out slide glass.
2. a kind of snap-type biochip according to claim 1, it is characterized in that described locked component is made of plastics, be provided with the first tooth limit in the described slot, described clamping block includes the second tooth limit, and the first tooth limit and the second tooth limit cooperatively interact clamping block is fixed in the described slot.
3. a kind of snap-type biochip according to claim 2, it is characterized in that the described first tooth limit comprises first plane and first inclined-plane, the described second tooth limit comprises second plane and second inclined-plane, described first plane is vertical with the direction of insertion of clamping block with second plane, and second plane on first plane on the first tooth limit and the second tooth limit cooperatively interacts just that clamping block is fixed in the described slot.
4. a kind of snap-type biochip according to claim 3 is characterized in that the thickness of described easy breaking off section is less than the thickness at locked component rear portion.
5. a kind of snap-type biochip according to claim 4, it is characterized in that described thin slice one side is provided with groove, groove on the thin slice is close on the described slide glass towards slide glass and with thin slice, makes and forms an airtight microfluidic channel between thin slice and the slide glass; Offer the fluid hole and the inlet opening that are communicated with described microfluidic channel on the described thin slice.
6. a kind of snap-type biochip according to claim 5 is characterized in that being provided with in the described upward enclosing cover and holds frame, and described thin slice is arranged on described holding in the frame.
7. a kind of snap-type biochip according to claim 6, it is characterized in that being close on the thin slice that face of slide glass and be provided with first sealed strip, described first sealed strip is positioned at the upper edge of groove, first sealed strip and slide glass closely cooperate, the medial surface of following enclosing cover is provided with second sealed strip, and second sealed strip and slide glass closely cooperate.
8. a kind of snap-type biochip according to claim 7 is characterized in that described enclosing cover down offers the empty avoiding window that can embed magnetic substance, described empty avoiding window be positioned at microfluidic channel under.
9. a kind of snap-type biochip according to claim 5, it is characterized in that being connected with on the described fluid hole first pipe joint element, be connected with second pipe joint element on the described inlet opening, described first pipe joint element and second pipe joint element are bonded on the described thin slice.
10. a kind of snap-type biochip according to claim 9, its feature comprises bottom and following four side at following enclosing cover, one end of following enclosing cover is provided with the small gap that convenient finger fetches slide glass, and this small gap one is distributed on the first side on bottom and the following four side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310145525XA CN103255055A (en) | 2013-04-24 | 2013-04-24 | Buckle type biological chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310145525XA CN103255055A (en) | 2013-04-24 | 2013-04-24 | Buckle type biological chip |
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| Publication Number | Publication Date |
|---|---|
| CN103255055A true CN103255055A (en) | 2013-08-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310145525XA Pending CN103255055A (en) | 2013-04-24 | 2013-04-24 | Buckle type biological chip |
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| CN (1) | CN103255055A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107922910A (en) * | 2016-07-29 | 2018-04-17 | 苏文弘 | Microfluidic device and application thereof and application method |
| CN109580537A (en) * | 2018-12-17 | 2019-04-05 | 南京市江宁医院 | Device based on terahertz time-domain spectroscopic technology detection viable bacteria |
| CN109943473A (en) * | 2019-04-02 | 2019-06-28 | 邦睿生技股份有限公司 | Inspection test piece device |
| CN115635439A (en) * | 2022-12-23 | 2023-01-24 | 微纳动力(北京)科技有限责任公司 | Micro-fluidic chip anchor clamps |
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| JPH06213789A (en) * | 1992-09-29 | 1994-08-05 | F Hoffmann La Roche Ag | Device for attaching cytological substance to microscope slide and for dyeing |
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| CN2931298Y (en) * | 2006-07-07 | 2007-08-08 | 唐显仕 | Plastic band |
| US20090226957A1 (en) * | 2005-03-24 | 2009-09-10 | Patrizia Paterlini-Brechot | Process and device for separating biological particles contained in a fluid by means of filtration |
| CN102965276A (en) * | 2012-11-20 | 2013-03-13 | 宁波美晶医疗技术有限公司 | Biochip for screening rare cells in blood |
| CN202848309U (en) * | 2012-07-31 | 2013-04-03 | 宁波飞天伟业工具有限公司 | Lock catch mechanism of packing box |
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2013
- 2013-04-24 CN CN201310145525XA patent/CN103255055A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2096931U (en) * | 1991-07-13 | 1992-02-26 | 姚少臣 | Disposable quick meal box |
| JPH06213789A (en) * | 1992-09-29 | 1994-08-05 | F Hoffmann La Roche Ag | Device for attaching cytological substance to microscope slide and for dyeing |
| CN2279093Y (en) * | 1997-03-03 | 1998-04-22 | 东莞艺爵包装盒有限公司 | Antitheft watch show box |
| US20090226957A1 (en) * | 2005-03-24 | 2009-09-10 | Patrizia Paterlini-Brechot | Process and device for separating biological particles contained in a fluid by means of filtration |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107922910A (en) * | 2016-07-29 | 2018-04-17 | 苏文弘 | Microfluidic device and application thereof and application method |
| CN109580537A (en) * | 2018-12-17 | 2019-04-05 | 南京市江宁医院 | Device based on terahertz time-domain spectroscopic technology detection viable bacteria |
| CN109943473A (en) * | 2019-04-02 | 2019-06-28 | 邦睿生技股份有限公司 | Inspection test piece device |
| CN109943473B (en) * | 2019-04-02 | 2022-04-05 | 邦睿生技股份有限公司 | Test strip device |
| CN115635439A (en) * | 2022-12-23 | 2023-01-24 | 微纳动力(北京)科技有限责任公司 | Micro-fluidic chip anchor clamps |
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Application publication date: 20130821 |