CN203238273U - Wing-shaped turnover cover type detachable biochip - Google Patents
Wing-shaped turnover cover type detachable biochip Download PDFInfo
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- CN203238273U CN203238273U CN 201320213165 CN201320213165U CN203238273U CN 203238273 U CN203238273 U CN 203238273U CN 201320213165 CN201320213165 CN 201320213165 CN 201320213165 U CN201320213165 U CN 201320213165U CN 203238273 U CN203238273 U CN 203238273U
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- thin slice
- slide glass
- housing
- biochip
- enclosing cover
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Abstract
The utility model discloses a wing-shaped turnover cover type detachable biochip. The biochip comprises a shell, a flake and a glass slide, wherein the flake and the glass slide are arranged in the shell; the shell comprises a tear tape and a fall-off surface which are integrally arranged; and the fall-off surface is defined by the tear tape and is separated from the shell by tearing off the tear tape, so that an open nick is left on the shell and the glass slide can be taken out of the open nick of the shell. The biochip has the advantages that as the flake and the glass slide are connected in a detachable manner, the fall-off surface is separated from the shell by tearing off the tear tape, so that the open nick is left on the shell and the glass slide can be taken out of the open nick of the shell for subsequent analysis of the rare cells on the glass slide. The biochip has a simpler structure and is very convenient to operate.
Description
Technical field
The utility model relates to a kind of blood testing biochip, especially relates to the detachable biochip of a kind of aerofoil profile flip-shell.
Background technology
The blood testing biochip is the diagnostic tool of future generation that detects rare cell in the blood relevant with major disease, and rare cell comprises cell with specified protein mark of circulating tumor cell (CTC), pernicious stem cell and the pathology of circulation in blood sample etc.Relevant studies confirm that the circulating tumor cell quantity that occurs in patient's the blood sample and patient's early diagnosis and survival rate have very strong dependency.Therefore, be the key that improves tumor disease early discovery rate and personalized treatment to the rare cell determination and analysis of patient's blood sample.
Because the CTCs quantity of patient blood sample is very little, CTC is detected have very large challenge.The flow cytometer detection method is a kind of CTC detection method of the most generally using, and CTC is larger than general cell space, and the caryoplasm ratio is high, the endochylema no particulate matter is few, expression specificity antigen, its characteristic to scattering of light and immunofluorescence identification of flow cytometry is separated CTC from total cell.Present up-to-date flow cytometer BD FACSAria is the new breakthrough of streaming high speed cell sorting, but its defective that is difficult to overcome is: instantaneous laser hits will bring damage to cell, the sorting poor activity; Because the intersection that exists between spectrum is easily sneaked into false-positive cell; The required compensation of fluorescent signal is difficult to allotment; Tend to occur the agglomerating phenomenon of cytoadherence, the obstruction of efferent tract happens occasionally; The filtration cell of having no progeny in the sorting again prepares sample, and this has increased the probability of polluting, and cytoactive is greatly impaired.Still deposit dispute with the method for the CTC in the Flow cytometry tumour patient merely; Other form fractionation method, the quantity of isolated CTCs and density are smaller from white corpuscle, can stay simultaneously a large amount of similar and be not enough to be considered as the cell of CTC with the CTC form, such as the same little acyclic tumour cell with white corpuscle.Also have immunologic detection method, because CTCs has the high special effect property of separating, can utilize specific marker, target CTC is carried out mark, but need the extra immunofluorescence dyeing screening step that increases.
So, be necessary to provide a kind of simple in structure, easy to operate blood testing biochip from the patient blood sample, to separate the specific protein cell plastid of rare cell and/or pathology.
Summary of the invention
Technical problem to be solved in the utility model provides a kind of simple in structure, easy to operate detachable biochip of aerofoil profile flip-shell that is used for screening blood rare cell.
The utility model solves the problems of the technologies described above the technical scheme that adopts:
The detachable biochip of a kind of aerofoil profile flip-shell, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in the described housing, described housing two middle side parts are provided with the outer teat on the broad ways, described housing comprises enclosing cover and lower enclosing cover, the medial surface of described upper enclosing cover is pressed on the upper surface of thin slice, the medial surface of described lower enclosing cover is pressed on the lower surface of slide glass, the face that comes off that is provided with tearing strip on the described housing and is surrounded by described tearing strip, tear tearing strip off, the face that comes off is separated from housing, stay next open breach at housing, described open breach comprises the conveying end and the convenient district that holds that stretches into that points that adapt with slide glass, and holds the district and is positioned at described outer teat, and slide glass can take out from the conveying end of housing.Housing plays the effect of protection thin slice and slide glass, and the simultaneously interaction by upper enclosing cover and lower enclosing cover fits tightly thin slice and slide glass and interfixes; form airtight microfluidic channel; after tearing strip tore off, slide glass can take out in housing, was used for subsequent analysis and processed.
Described thin slice one side is provided with groove, and the groove on the thin slice is close on the described slide glass towards slide glass and with thin slice, makes and forms an airtight microfluidic channel between thin slice and the slide glass; Offer the fluid hole and the inlet opening that are communicated with described microfluidic channel on the described thin slice.
Described upper enclosing cover comprises base and upper four side, and tearing strip is distributed in the base and wherein on the side, the face of coming off comprises most of zone of base and this side.
Described tearing strip is two zone bands that the cutting that is parallel to each other surrounds on the housing, tearing strip comprises two initiating terminals, two initiating terminals are positioned at the bottom of this side, and tearing strip is crossed by the initiating terminal far-end that extends to upper base that sets out, and is provided with handle section on the described initiating terminal.
Be close on the thin slice on that face of slide glass and be provided with the first sealed strip, described the first sealed strip is positioned at the upper edge of groove, and the first sealed strip and slide glass closely cooperate, and the medial surface of lower enclosing cover is provided with the second sealed strip, and the second sealed strip and slide glass closely cooperate.The setting of the first sealing-ring forms airtight microfluidic channel, avoids blood leakage.Housing pushes slide glass by the second sealed strip, can prevent that slide glass from breaking because of extruding simultaneously.
Described lower enclosing cover offers the empty avoiding window that can embed magnetic substance, described empty avoiding window be positioned at microfluidic channel under.The magnet that is used to form gradient magnetic is placed on the below of slide glass, and offering of empty avoiding window can avoid housing to stop magnetic field, make magnetic field more useful effect in the blood sample of the microfluidic channel of flowing through.
Be connected with the first pipe joint element on the described fluid hole, be connected with the second pipe joint element on the described inlet opening, described the first pipe joint element and the second pipe joint element are bonded on the described thin slice.Blood. reservoir is communicated with the first pipe joint element by pipe connecting, the second pipe joint element is communicated with liquor pump by pipe connecting, under the suction of liquor pump, blood sample in the storer is in the first pipe joint element flows into microfluidic channel, blood sample in the microfluidic channel flows to liquor pump through the second pipe joint element, and blood sample is with a certain suitable speed runoff microfluidic channel.
Described housing has plastics to make, and described thin slice is made by silica gel material.
Described upper enclosing cover and described thin slice integrated injection molding.First housing is carried out injection moulding, then with thin slice and housing secondary injection molding.
Described upper outside covering is provided with container cavity, and described thin slice is positioned at described container cavity.
Compared with prior art, the utility model has the advantages that and in blood sample, put into first magnetic nano-particle, the magnetic nano-particle surface is with antibody, be adsorbed on the target cell by immune response, then the blood sample that rare cell has been labeled the microfluidic channel of flowing through, the rare cell that is attached with magnetic nano-particle in the blood sample is placed on the gradient magnetic guiding that the magnetic substance below the biochip sends, and then be collected on the slide glass, since between thin slice and the slide glass for removably connecting, tear tearing strip off, the face that comes off is separated from housing, stay next open breach at housing, open breach comprises the conveying end and the convenient district that holds that stretches into that points that adapt with slide glass, and holds the district and is positioned at outer teat, and slide glass can take out from the conveying end of housing.Rare cell on the slide glass is carried out subsequent analysis.The utility model is relatively simple for structure, and it is very convenient to operate.
Description of drawings
Fig. 1 is shape assumption diagram of the present utility model;
State graph when Fig. 2 is tearing strip pull-up of the present utility model;
Fig. 3 is exploded view of the present utility model (one);
Fig. 4 is that disengaging face of the present utility model is together with the separated structure iron of thin slice;
Fig. 5 is exploded view of the present utility model (two).
Embodiment
Embodiment is described in further detail the utility model below in conjunction with accompanying drawing.
The detachable biochip of a kind of aerofoil profile flip-shell, comprise housing 1, thin slice 2 and slide glass 3, thin slice 2 and slide glass 3 are arranged in the housing 1,1 liang of middle side part of housing is provided with the outer teat 4 on the broad ways, housing 1 comprises enclosing cover 5 and lower enclosing cover 6, the medial surface of upper enclosing cover 5 is pressed on the upper surface of thin slice 2, the medial surface of lower enclosing cover 6 is pressed on the lower surface of slide glass 3, the face that comes off 8 that is provided with tearing strip 7 on the housing 1 and is surrounded by tearing strip 7, tear tearing strip 7 off, the face 8 that comes off is separated from housing 1, stay next open breach at housing 1, open breach comprises that a conveying end 9 that adapts with slide glass 3 and convenient finger stretch into holds district 10, holds district 10 and is positioned at outer teat 4, and slide glass 3 can be from conveying end 9 taking-ups of housing 1.
Upper enclosing cover 5 comprises base 51 and upper four side, and tearing strip 7 is distributed in base 51 and wherein on the side 52, the face 8 that comes off comprises the most of zone on base 51 and this side 52.
Upper enclosing cover 5 and thin slice 2 integrated injection moldings.After tearing strip was pulled away from, disengaging face was separated together with thin slice.
Be close on the thin slice 2 on slide glass 3 those faces and be provided with the first sealed strip 15, the first sealed strip 15 is positioned at the upper edge of groove 11, the first sealed strip 15 closely cooperates with slide glass 3, and the medial surface of lower enclosing cover 6 is provided with the second sealed strip 17, the second sealed strips 17 and closely cooperates with slide glass 3.
Lower enclosing cover 6 offers the empty avoiding window 18 that can embed magnetic substance, empty avoiding window 18 be positioned at microfluidic channel under.
Be connected with the first pipe joint element 19 on the fluid hole, be connected with the second pipe joint element 20, the first pipe joint elements 19 and the second pipe joint element 20 on the inlet opening and be bonded on the thin slice.
Housing has plastics to make, and thin slice 2 is made by silica gel material.
Be provided with container cavity 21 on the upper enclosing cover 5, thin slice 2 is positioned at container cavity 21.
The tackiness agent that pipe joint element and thin slice 2 adopt, requirement is to produce any impact to blood sample, guarantees simultaneously the stopping property of system, prevents the blood sample leakage.The thickness of thin slice 2 is 8mm or 10mm or 12mm, and the degree of depth of groove 11 is 0.4mm or 0.5mm or 0.6mm, and the diameter of bore of inlet opening and fluid hole is 1.5mm or 1.58mm or 1.7mm.
Claims (10)
1. detachable biochip of aerofoil profile flip-shell, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in the described housing, it is characterized in that described housing two middle side parts are provided with the outer teat on the broad ways, described housing comprises enclosing cover and lower enclosing cover, the medial surface of described upper enclosing cover is pressed on the upper surface of thin slice, the medial surface of described lower enclosing cover is pressed on the lower surface of slide glass, the face that comes off that is provided with tearing strip on the described housing and is surrounded by described tearing strip, tear tearing strip off, the face that comes off is separated from housing, stay next open breach at housing, described open breach comprises the conveying end and the convenient district that holds that stretches into that points that adapt with slide glass, and holds the district and is positioned at described outer teat, and slide glass can take out from the conveying end of housing.
2. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 1, it is characterized in that described thin slice one side is provided with groove, groove on the thin slice is close on the described slide glass towards slide glass and with thin slice, makes and forms an airtight microfluidic channel between thin slice and the slide glass; Offer the fluid hole and the inlet opening that are communicated with described microfluidic channel on the described thin slice.
3. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 2, it is characterized in that described upper enclosing cover comprises base and upper four side, tearing strip is distributed in the base and wherein on the side, the face of coming off comprises most of zone of base and this side.
4. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 3, it is characterized in that described tearing strip is two zone bands that the cutting that is parallel to each other surrounds on the housing, tearing strip comprises two initiating terminals, two initiating terminals are positioned at the bottom of this side, tearing strip is crossed by the initiating terminal far-end that extends to upper base that sets out, and is provided with handle section on the described initiating terminal.
5. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 4, it is characterized in that being close on the thin slice on that face of slide glass and be provided with the first sealed strip, described the first sealed strip is positioned at the upper edge of groove, the first sealed strip and slide glass closely cooperate, the medial surface of lower enclosing cover is provided with the second sealed strip, and the second sealed strip and slide glass closely cooperate.
6. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 5 is characterized in that described lower enclosing cover offers the empty avoiding window that can embed magnetic substance, described empty avoiding window be positioned at microfluidic channel under.
7. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 2, it is characterized in that being connected with on the described fluid hole the first pipe joint element, be connected with the second pipe joint element on the described inlet opening, described the first pipe joint element and the second pipe joint element are bonded on the described thin slice.
8. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 7 is characterized in that described housing has plastics to make, and described thin slice is made by silica gel material.
9. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 8 is characterized in that described upper enclosing cover and described thin slice integrated injection molding.
10. the detachable biochip of a kind of aerofoil profile flip-shell according to claim 9 is characterized in that described upper outside covering is provided with container cavity, and described thin slice is positioned at described container cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320213165 CN203238273U (en) | 2013-04-24 | 2013-04-24 | Wing-shaped turnover cover type detachable biochip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320213165 CN203238273U (en) | 2013-04-24 | 2013-04-24 | Wing-shaped turnover cover type detachable biochip |
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| Publication Number | Publication Date |
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| CN203238273U true CN203238273U (en) | 2013-10-16 |
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| CN 201320213165 Expired - Lifetime CN203238273U (en) | 2013-04-24 | 2013-04-24 | Wing-shaped turnover cover type detachable biochip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104792701A (en) * | 2015-03-17 | 2015-07-22 | 中国科学院物理研究所 | Micro-sample cell |
| CN105572046A (en) * | 2014-10-16 | 2016-05-11 | 中国科学院物理研究所 | Fluorescence detection sample pool and preparation method thereof |
-
2013
- 2013-04-24 CN CN 201320213165 patent/CN203238273U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572046A (en) * | 2014-10-16 | 2016-05-11 | 中国科学院物理研究所 | Fluorescence detection sample pool and preparation method thereof |
| CN105572046B (en) * | 2014-10-16 | 2018-06-26 | 中国科学院物理研究所 | Fluoroscopic examination sample cell and preparation method thereof |
| CN104792701A (en) * | 2015-03-17 | 2015-07-22 | 中国科学院物理研究所 | Micro-sample cell |
| CN104792701B (en) * | 2015-03-17 | 2017-11-21 | 中国科学院物理研究所 | Micro-example pond |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131016 |