CN203238271U - Easy-to-pull detachable biochip - Google Patents
Easy-to-pull detachable biochip Download PDFInfo
- Publication number
- CN203238271U CN203238271U CN 201320212626 CN201320212626U CN203238271U CN 203238271 U CN203238271 U CN 203238271U CN 201320212626 CN201320212626 CN 201320212626 CN 201320212626 U CN201320212626 U CN 201320212626U CN 203238271 U CN203238271 U CN 203238271U
- Authority
- CN
- China
- Prior art keywords
- thin slice
- slide glass
- housing
- enclosing cover
- easy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses an easy-to-pull detachable biochip. The biochip comprises a shell, a flake and a glass slide, wherein the flake and the glass slide are arranged in the shell; the shell comprises a tear tape and a fall-off surface which are integrally arranged; and the fall-off surface is defined by the tear tape and is separated from the shell by tearing off the tear tape, so that an open nick is left on the shell and the glass slide can be taken out of the open nick of the shell. The biochip has the advantages that as the flake and the glass slide are connected in a detachable manner, the fall-off surface is separated from the shell by tearing off the tear tape, so that the open nick is left on the shell and the glass slide can be taken out of the open nick of the shell for subsequent analysis of the rare cells on the glass slide. The biochip has a simpler structure and is very convenient to operate.
Description
Technical field
The utility model relates to a kind of blood testing biochip, especially relates to the detachable biochip of a kind of easy-to-pull type.
Background technology
The blood testing biochip is the diagnostic tool of future generation that detects rare cell in the blood relevant with major disease, and rare cell comprises cell with specified protein mark of circulating tumor cell (CTC), pernicious stem cell and the pathology of circulation in blood sample etc.Relevant studies confirm that the circulating tumor cell quantity that occurs in patient's the blood sample and patient's early diagnosis and survival rate have very strong dependency.Therefore, be the key that improves tumor disease early discovery rate and personalized treatment to the rare cell determination and analysis of patient's blood sample.
Because the CTCs quantity of patient blood sample is very little, CTC is detected have very large challenge.The flow cytometer detection method is a kind of CTC detection method of the most generally using, and CTC is larger than general cell space, and the caryoplasm ratio is high, the endochylema no particulate matter is few, expression specificity antigen, its characteristic to scattering of light and immunofluorescence identification of flow cytometry is separated CTC from total cell.Present up-to-date flow cytometer BD FACSAria is the new breakthrough of streaming high speed cell sorting, but its defective that is difficult to overcome is: instantaneous laser hits will bring damage to cell, the sorting poor activity; Because the intersection that exists between spectrum is easily sneaked into false-positive cell; The required compensation of fluorescent signal is difficult to allotment; Tend to occur the agglomerating phenomenon of cytoadherence, the obstruction of efferent tract happens occasionally; The filtration cell of having no progeny in the sorting again prepares sample, and this has increased the probability of polluting, and cytoactive is greatly impaired.Still deposit dispute with the method for the CTC in the Flow cytometry tumour patient merely; Other form fractionation method, the quantity of isolated CTCs and density are smaller from white corpuscle, can stay simultaneously a large amount of similar and be not enough to be considered as the cell of CTC with the CTC form, such as the same little acyclic tumour cell with white corpuscle.Also have immunologic detection method, because CTCs has the high special effect property of separating, can utilize specific marker, target CTC is carried out mark, but need the extra immunofluorescence dyeing screening step that increases.
So, be necessary to provide a kind of simple in structure, easy to operate blood testing biochip from the patient blood sample, to separate the specific protein cell plastid of rare cell and/or pathology.
Summary of the invention
Technical problem to be solved in the utility model provides a kind of simple in structure, easy to operate detachable biochip of easy-to-pull type that is used for screening blood rare cell.
The utility model solves the problems of the technologies described above the technical scheme that adopts: the detachable biochip of a kind of easy-to-pull type, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in the described housing, it is characterized in that described housing includes the tearing strip that is wholely set and the face that comes off that is surrounded by described tearing strip, tear tearing strip off, the face that comes off is separated from housing, stays next open breach at housing, and slide glass can take out from the open indentation, there of housing.
Described thin slice one side is provided with groove, and the groove on the thin slice is close on the described slide glass towards slide glass and with thin slice, makes and forms an airtight microfluidic channel between thin slice and the slide glass; Offer the fluid hole and the inlet opening that are communicated with described microfluidic channel on the described thin slice.
Housing comprises that the medial surface of described upper enclosing cover is pressed on the upper surface of thin slice by upper enclosing cover and lower enclosing cover, and the medial surface of described lower enclosing cover is pressed on the lower surface of slide glass.Housing plays the effect of protection thin slice and slide glass, and the simultaneously interaction by upper enclosing cover and lower enclosing cover fits tightly thin slice and slide glass and interfixes; form airtight microfluidic channel; after tearing strip tore off, slide glass can take out in housing, was used for subsequent analysis and processed.
Lower enclosing cover comprises bottom and lower four side, one end of lower enclosing cover is provided with the small gap that convenient finger fetches slide glass, this small gap one is distributed on the first side on bottom and the lower four side, described upper enclosing cover comprises base and upper four side, tearing strip is distributed on the second side of base and upper four side, the face of coming off comprises most of zone of base and second side, and first side and second side are positioned at the same side of housing.After tearing strip tore off, open breach appearred in upper base and second side, and lower outside covering has small gap, can easily slide glass be taken out in housing.
Described tearing strip is two zone bands that the cutting that is parallel to each other surrounds on the housing, tearing strip comprises two initiating terminals, two initiating terminals are positioned at the bottom of second side, and tearing strip is crossed by the initiating terminal far-end that extends to upper base that sets out, and is provided with handle section on the described initiating terminal.
Described upper enclosing cover and described thin slice integrated injection molding.First housing is carried out injection moulding, then with thin slice and housing secondary injection molding.
Be close on the thin slice on that face of slide glass and be provided with the first sealed strip, described the first sealed strip is positioned at the upper edge of groove, and the first sealed strip and slide glass closely cooperate, and the medial surface of lower enclosing cover is provided with the second sealed strip, and the second sealed strip and slide glass closely cooperate.The setting of the first sealing-ring forms airtight microfluidic channel, avoids blood leakage.Housing pushes slide glass by the second sealed strip, can prevent that slide glass from breaking because of extruding simultaneously.
Described lower enclosing cover offers the empty avoiding window that can embed magnetic substance, described empty avoiding window be positioned at microfluidic channel under.The magnet that is used to form gradient magnetic is placed on the below of slide glass, and offering of empty avoiding window can avoid housing to stop magnetic field, make magnetic field more useful effect in the blood sample of the microfluidic channel of flowing through.
Be connected with the first pipe joint element on the described fluid hole, be connected with the second pipe joint element on the described inlet opening, described the first pipe joint element and the second pipe joint element are bonded on the described thin slice.Blood. reservoir is communicated with the first pipe joint element by pipe connecting, the second pipe joint element is communicated with liquor pump by pipe connecting, under the suction of liquor pump, blood sample in the storer is in the first pipe joint element flows into microfluidic channel, blood sample in the microfluidic channel flows to liquor pump through the second pipe joint element, and blood sample is with a certain suitable speed runoff microfluidic channel.
Described housing has plastics to make, and described thin slice is made by silica gel material.
Compared with prior art, the utility model has the advantages that and in blood sample, put into first magnetic nano-particle, the magnetic nano-particle surface is with antibody, be adsorbed on the target cell by immune response, then the blood sample that rare cell has been labeled the microfluidic channel of flowing through, the rare cell that is attached with magnetic nano-particle in the blood sample is placed on the gradient magnetic guiding that the magnetic substance below the biochip sends, and then be collected on the slide glass, since between thin slice and the slide glass for removably connecting, tear tearing strip off, the face that comes off is separated from housing, stay next open breach at housing, slide glass can take out from the open indentation, there of housing, and the rare cell on the slide glass is carried out subsequent analysis.The utility model is relatively simple for structure, and it is very convenient to operate.
Description of drawings
Fig. 1 is structure iron of the present utility model;
State graph when Fig. 2 is tearing strip pull-up of the present utility model;
Fig. 3 is upper enclosing cover of the present utility model and the lower enclosing cover cut-away view () when opening;
Fig. 4 is upper enclosing cover of the present utility model and the lower enclosing cover cut-away view (two) when opening;
Fig. 5 is the structure iron after slide glass separates among Fig. 4;
Fig. 6 is the exploded view after tearing strip of the present utility model pulls off;
Fig. 7 is exploded view of the present utility model.
Embodiment
Embodiment is described in further detail the utility model below in conjunction with accompanying drawing.
The detachable biochip of a kind of easy-to-pull type, comprise housing 1, thin slice 2 and slide glass 3, thin slice 2 and slide glass 3 are arranged in the housing 1, housing 1 includes the tearing strip 4 that is wholely set and the face that comes off 5 that is surrounded by tearing strip 4, tear tearing strip 4 off, the face 5 that comes off is separated from housing 1, stays next open breach 6 at housing 1, and slide glass 3 can take out from the open breach 6 of housing 1.Wave carrier piece can adopt pathology level slide glass.
Housing 1 comprises that by upper enclosing cover 11 and lower enclosing cover 12, upper enclosing cover 11 and lower enclosing cover 12 interfix, and can't open, and the medial surface of upper enclosing cover 11 is pressed on the upper surface of thin slice 2, and the medial surface of lower enclosing cover 12 is pressed on the lower surface of slide glass 3.Housing can only use once, after tearing strip tears off, just scraps with regard to housing and thin slice.Enclosing cover and lower enclosing cover are opened on Fig. 3, Fig. 4 and Fig. 5, are in order to express internal structure.
Lower enclosing cover 12 comprises bottom 121 and lower four side, one end of lower enclosing cover 12 is provided with the small gap 122 that convenient finger fetches slide glass, these small gap 122 one are distributed on the first side 123 on bottom 121 and the lower four side, upper enclosing cover 11 comprises base 111 and upper four side, tearing strip 4 is distributed on the second side 112 of base 111 and upper four side, the face 5 that comes off comprises most of zone of base 111 and second side 112, and first side 123 and second side 112 are positioned at the same side of housing 1.
Upper enclosing cover 11 and thin slice 2 integrated injection moldings.
Be close on the thin slice 2 on slide glass 3 those faces and be provided with the first sealed strip 8, the first sealed strip 8 is positioned at the upper edge of groove 7, the first sealed strip 8 closely cooperates with slide glass 3, and the medial surface of lower enclosing cover 11 is provided with the second sealed strip 9, the second sealed strips 9 and closely cooperates with slide glass 3.
Lower enclosing cover 12 offers the empty avoiding window 13 that can embed magnetic substance, empty avoiding window 13 be positioned at microfluidic channel under.
Be connected with the first pipe joint element 14 on the fluid hole, be connected with the second pipe joint element 15, the first pipe joint elements 14 and the second pipe joint element 15 on the inlet opening and be bonded on the thin slice 2.
Housing 1 has plastics to make, and thin slice 2 is made by silica gel material.
The tackiness agent that pipe joint element and thin slice 2 adopt, requirement is to produce any impact to blood sample, guarantees simultaneously the stopping property of system, prevents the blood sample leakage.The thickness of thin slice 2 is 8mm or 10mm or 12mm, and the degree of depth of groove 7 is 0.4mm or 0.5mm or 0.6mm, and the diameter of bore of inlet opening and fluid hole is 1.5mm or 1.58mm or 1.7mm.
Claims (10)
1. detachable biochip of easy-to-pull type, comprise housing, thin slice and slide glass, described thin slice and slide glass are arranged in the described housing, it is characterized in that described housing includes the tearing strip that is wholely set and the face that comes off that is surrounded by described tearing strip, tear tearing strip off, the face that comes off is separated from housing, stays next open breach at housing, and slide glass can take out from the open indentation, there of housing.
2. the detachable biochip of a kind of easy-to-pull type according to claim 1, it is characterized in that described thin slice one side is provided with groove, groove on the thin slice is close on the described slide glass towards slide glass and with thin slice, makes and forms an airtight microfluidic channel between thin slice and the slide glass; Offer the fluid hole and the inlet opening that are communicated with described microfluidic channel on the described thin slice.
3. the detachable biochip of a kind of easy-to-pull type according to claim 1, it is characterized in that housing comprises by upper enclosing cover and lower enclosing cover, the medial surface of described upper enclosing cover is pressed on the upper surface of thin slice, and the medial surface of described lower enclosing cover is pressed on the lower surface of slide glass.
4. the detachable biochip of a kind of easy-to-pull type according to claim 3, it is characterized in that lower enclosing cover comprises bottom and lower four side, one end of lower enclosing cover is provided with the small gap that convenient finger fetches slide glass, this small gap one is distributed on the first side on bottom and the lower four side, described upper enclosing cover comprises base and upper four side, tearing strip is distributed on the second side of base and upper four side, the face of coming off comprises most of zone of base and second side, and first side and second side are positioned at the same side of housing.
5. the detachable biochip of a kind of easy-to-pull type according to claim 4, it is characterized in that described tearing strip is two zone bands that the cutting that is parallel to each other surrounds on the housing, tearing strip comprises two initiating terminals, two initiating terminals are positioned at the bottom of second side, tearing strip is crossed by the initiating terminal far-end that extends to upper base that sets out, and is provided with handle section on the described initiating terminal.
6. the detachable biochip of a kind of easy-to-pull type according to claim 5 is characterized in that described upper enclosing cover and described thin slice integrated injection molding.
7. the detachable biochip of a kind of easy-to-pull type according to claim 6, it is characterized in that being close on the thin slice on that face of slide glass and be provided with the first sealed strip, described the first sealed strip is positioned at the upper edge of groove, the first sealed strip and slide glass closely cooperate, the medial surface of lower enclosing cover is provided with the second sealed strip, and the second sealed strip and slide glass closely cooperate.
8. the detachable biochip of a kind of easy-to-pull type according to claim 7 is characterized in that described lower enclosing cover offers the empty avoiding window that can embed magnetic substance, described empty avoiding window be positioned at microfluidic channel under.
9. the detachable biochip of a kind of easy-to-pull type according to claim 2, it is characterized in that being connected with on the described fluid hole the first pipe joint element, be connected with the second pipe joint element on the described inlet opening, described the first pipe joint element and the second pipe joint element are bonded on the described thin slice.
10. the detachable biochip of a kind of easy-to-pull type according to claim 9 is characterized in that described housing has plastics to make, and described thin slice is made by silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320212626 CN203238271U (en) | 2013-04-24 | 2013-04-24 | Easy-to-pull detachable biochip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320212626 CN203238271U (en) | 2013-04-24 | 2013-04-24 | Easy-to-pull detachable biochip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203238271U true CN203238271U (en) | 2013-10-16 |
Family
ID=49315588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320212626 Expired - Lifetime CN203238271U (en) | 2013-04-24 | 2013-04-24 | Easy-to-pull detachable biochip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203238271U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104552679A (en) * | 2015-01-12 | 2015-04-29 | 北京同方生物芯片技术有限公司 | Preparation method of mold core of mold, mold core of mold and biochip |
| CN109055204A (en) * | 2018-10-19 | 2018-12-21 | 杭州捷诺飞生物科技股份有限公司 | Drug screening organ chip |
-
2013
- 2013-04-24 CN CN 201320212626 patent/CN203238271U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104552679A (en) * | 2015-01-12 | 2015-04-29 | 北京同方生物芯片技术有限公司 | Preparation method of mold core of mold, mold core of mold and biochip |
| CN104552679B (en) * | 2015-01-12 | 2017-01-11 | 北京同方生物芯片技术有限公司 | Preparation method of mold core of mold, mold core of mold and biochip |
| CN109055204A (en) * | 2018-10-19 | 2018-12-21 | 杭州捷诺飞生物科技股份有限公司 | Drug screening organ chip |
| CN109055204B (en) * | 2018-10-19 | 2024-03-26 | 杭州捷诺飞生物科技股份有限公司 | Organ chip for drug screening |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102965276A (en) | Biochip for screening rare cells in blood | |
| JP2022043287A (en) | Assay method using different spacing heights | |
| CN105242004B (en) | Ensemble-decision aliquot ranking | |
| CN205517817U (en) | Prevent little valve of refluence | |
| Lv et al. | Spatially gradated segregation and recovery of circulating tumor cells from peripheral blood of cancer patients | |
| CN106635995A (en) | Circulating tumor cell negative enrichment method | |
| JP2009109514A (en) | Analysis of circulating tumor cell, fragment, and debris | |
| CN103589629A (en) | Separation system for CTCs (circulating tumor cells) | |
| KR101636316B1 (en) | Microfluidic apparatus for isolation, method for isolation using the same, and isolation kit for circulating rare cells using the same | |
| Khoo et al. | Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip | |
| CN203238271U (en) | Easy-to-pull detachable biochip | |
| Raillon et al. | Toward microfluidic label‐free isolation and enumeration of circulating tumor cells from blood samples | |
| CN203238273U (en) | Wing-shaped turnover cover type detachable biochip | |
| US20150233932A1 (en) | Methods, Systems, and Compositions for Enrichment of Rare Cells | |
| Hakim et al. | A new insight to deformability correlation of circulating tumor cells with metastatic behavior by application of a new deformability-based microfluidic chip | |
| CN109187977A (en) | It is a kind of detect HER2 antigen different loci immunofluorescent reagent box and application | |
| CN103255055A (en) | Buckle type biological chip | |
| Shin et al. | Integrative Magneto‐Microfluidic Separation of Immune Cells Facilitates Clinical Functional Assays | |
| CN203625357U (en) | Circulating tumor cell separation system | |
| CN205517818U (en) | Little valve of choked flow | |
| CN109239030A (en) | A kind of kit and application detecting circulating tumor cell HER2 different loci and ER, PR | |
| CN109100504A (en) | A kind of platelet-leucocyte hybrid films coating immunomagnetic beads and the preparation method and application thereof | |
| CN103255056A (en) | Easy-pull type detachable biological chip | |
| CN201935921U (en) | Colloidal gold whole blood test card with U-shaped filter structure and three layers of blood separating films | |
| CN207143246U (en) | A kind of integrated form Terahertz superstructure nano biological sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131016 |