CN203021562U - Multipoint inoculation device - Google Patents
Multipoint inoculation device Download PDFInfo
- Publication number
- CN203021562U CN203021562U CN2013200222440U CN201320022244U CN203021562U CN 203021562 U CN203021562 U CN 203021562U CN 2013200222440 U CN2013200222440 U CN 2013200222440U CN 201320022244 U CN201320022244 U CN 201320022244U CN 203021562 U CN203021562 U CN 203021562U
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- cover plate
- connects
- frid
- multiple spot
- bacterium device
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Abstract
The utility model discloses a multipoint inoculation device. The multipoint inoculation device comprises a groove plate and a cover plate corresponding to the groove plate, wherein the groove plate is provided with at least two cylindrical grooves, the at least two grooves have the equal diameter and depth, the lower surface of the cover plate is provided with at least two inoculation columns with equal diameter and height, one ends of the inoculation columns are fixedly connected with the cover plate, the inoculation columns are inserted and matched with the grooves on the groove plate correspondingly, the multipoint inoculation device is characterized in that the groove plate and the cover plate are provided with positioning structures which correspond to each other. With the multipoint inoculation device, strains can be ensured to be extracted and accurately positioned while realizing multipoint inoculation with one-time operation, and the problem that the strains are extracted by mistake to cause inoculation mixture can be solved.
Description
Technical field
The utility model belongs to experiment apparatus technical field, is specifically related to a kind of multiple spot and connects the bacterium device.
Background technology
At present, the bacterium instrument that connects commonly used is transfering loop, inoculating needle, and front end is inserted in metal handle by the NI-G silk and fixedly forms, and the rear end is put the glue handle that do not readily conduct heat such as plastics and forms by the rear portion (position of holding) of metal handle.In the culture dish during inoculating strain, the shank of hand-held inoculating needle or transfering loop is sticking to be got bacterium liquid point and is connected on substratum; Perhaps serve as inoculating tool with aseptic pipettor gun head, get on the substratum of inoculation to be tried in culture dish with the rifle head is sticking.But, doing Physiology and biochemistry when experiment of a large amount of microorganism strains on the same substratum, just need a large amount of substratum of preparation, and need to connect bacterium one by one on the substratum of correspondence, not only waste a large amount of reagent chemicalses, also expend a large amount of manpowers and time, the analysis of making a large amount of physical and chemical indexs to researcher brings difficulty; And the connect bacterium amount of the common bacterium instrument that connects on substratum is difficult to hold, and is difficult to observe the colony characteristics of different strains normal growth on same culture dish, causes experimental result to be difficult to judgement.
In the prior art, patent CN2382371Y discloses a kind of medical multiple spot and has connect the bacterium device, in embodiment and accompanying drawing, this medical multiple spot of explanation connects the bacterium device, to be provided with screw thread and to be furnished with the through hole that the loop-carrier of nut passes on supporting plate by a plurality of ends to consist of, it can extract the bacterial classification of depositing in depression on notch board, this medical multiple spot connects the bacterium device can realize once that multiple spot connects bacterium, easy to use, but it is when operation, owing to there is no locating structure, be easy to occur the loop-carrier dislocation and extract and then cause the chaotic problem of inoculation, have a strong impact on and connect the bacterium effect.
Summary of the invention
The purpose of this utility model is to provide a kind of multiple spot and connects the bacterium device, and when realizing that the single job multiple spot connects bacterium, the extraction of assurance bacterial classification is the location accurately, solves the problem of the inoculation confusion that causes because of the extraction bacterial classification that misplaces.
In order to realize above purpose, the technical scheme that the utility model adopts is: a kind of multiple spot connects the bacterium device, comprise frid and the cover plate corresponding with frid, described frid is provided with at least 2 diameters columniform groove identical with the degree of depth, the lower surface of described cover plate is provided with at least 2 diameters and is fixedly connected with on the cover board the bacterial spawn that connects with highly identical and an end, describedly connect bacterial spawn grafting corresponding to groove on frid and coordinate, described frid and cover plate are provided with corresponding locating structure.
Multiple spot of the present utility model connects the bacterium device, corresponding locating structure is set on frid and cover plate, convenient connecing on cover plate when being pressed into the groove of frid, realized bacterial spawn accurately location, the bacterial spawn that connects on cover plate is inserted in groove corresponding on frid, target bacterial classification in groove is seeded on corresponding substratum, and unlikely appearance is because can't accurately locating the situation that connects bacterium mistake and confusion that occurs.
Described locating structure is the grafting locating structure, be located at junction of the edges of two sheets of paper groove or the roulette of frid and cover plate side.
Described grafting locating structure is by the groove that is located at respectively the irregular distribution on frid and cover plate and connect bacterial spawn and consist of.The bacterial spawn that connects on groove on frid and cover plate presents irregular distribution, and connecing corresponding the pegging graft of bacterial spawn and groove coordinates, during with connecing bacterial spawn and be pressed into groove on frid on cover plate, the direction of insertion and form are unique, so just do not have the problem that the bacterial spawn dislocation is extracted that connects.
Described grafting locating structure is made of backstay and the locating slot that mutual grafting coordinates.Described backstay is arranged on the side of cover plate, and described locating slot is arranged on the side of frid.The grafting locating structure that is made of backstay and locating slot makes cover plate and frid in the situation that dislocation can't be carried out bacterial classification extracts, and prevents because of carelessness connecing bacterial spawn and be pressed into just find it is the situation of the inoculation failure that causes of dislocation extraction after groove on frid on cover plate.
The upper surface of described cover plate is fixedly connected with cover plate and carries button.
It is tabular that described cover plate is carried button.Tabular cover plate is carried just hand-held of knob.
Described cover plate is put forward level one side of button towards described locating structure.
Described frid and cover plate are disc-shaped plate.Discoidal frid and cover plate are corresponding with culture dish commonly used.
Described frid and cover plate are glass fiber reinforced polyester tetrafluoroethylene plate.
The described bacterial spawn that connects is the stainless steel cylinder.
Multiple spot of the present utility model connects the bacterium device, and frid, cover plate, cover plate are carried button and connect bacterial spawn is all high temperature material, and (121 ℃ 30min), can not deform and damage can to carry out repeatedly high pressure steam sterilization in high-pressure steam sterilizing pan.
The use procedure that multiple spot of the present utility model connects the bacterium device is: in advance bacterial strain to be measured is made bacteria suspension, equivalent is clicked and entered in the groove of autoclaved frid; The locating structure of alignment frid and cover plate, hand-held cover plate is carried button, and the bacterial spawn that connects on autoclaved cover plate is pressed in groove corresponding to frid; Rear proposition is gently pressed the bacterial spawn that connects on cover plate on substratum, just with on the same substratum of multi-strain bacteria kind access, realizes that the single job multiple spot connects bacterium.
Multiple spot of the present utility model connects the bacterium device, and frid and cover plate are provided with corresponding locating structure, when realizing that the single job multiple spot connects bacterium, guarantees that bacterial classification extracts accurately location, solves the problem of extracting the inoculation confusion that causes because of the bacterial classification dislocation; Simple to operate, quick, easy to use, save reagent and medicine, save a large amount of human and material resources; Connecing bacterial strain after bacterium can rule and be evenly distributed on substratum, is convenient to observed result.
Description of drawings
Fig. 1 is that the master of a kind of embodiment of the present utility model looks sectional view;
Fig. 2 is the vertical view of frid in Fig. 1;
Fig. 3 is the upward view of Fig. 1 cover plate;
Fig. 4 is the vertical view of Fig. 1 cover plate;
Fig. 5 is that the master of a kind of improvement project of the present utility model looks sectional view;
Fig. 6 is the vertical view of frid in Fig. 5;
Fig. 7 is the upward view of Fig. 5 cover plate;
The number in the figure explanation: the 1-frid, the 2-cover plate, the 3-groove, the 4-cover plate is carried button, and 5-connects bacterial spawn, 6-junction of the edges of two sheets of paper groove, 7-backstay, 8-locating slot.
Embodiment
The utility model is described in further detail below in conjunction with embodiment.
Multiple spot of the present utility model connects a kind of embodiment of bacterium device, as shown in Fig. 1-4, comprises frid 1 and the cover plate 2 corresponding with frid 1, and this frid 1 and cover plate 2 are disc-shaped plate; Frid 1 is provided with 36 columniform grooves 3 that diameter is identical with the degree of depth, and these 36 grooves are concentric annular and are arranged in inside and outside 3 layers, are followed successively by from inside to outside 6,12 and 18; The upper surface of cover plate 2 is fixedly connected with cover plate and carries button 4, and cover plate is carried button 4 for semicircle tabular, and its plane corresponding with arc surface is fixedly connected with the upper surface of cover plate 2, and is convenient hand-held; The lower surface of cover plate 2 is provided with 36 diameters and a highly identical end and is fixedly connected on and connects bacterial spawn 5 on cover plate 2, connect bacterial spawn 5 and the corresponding cooperation that arranges and peg graft of groove 3 on frid 1, these 36 to connect the arrangement mode of bacterial spawn identical with groove 3 on frid 1, and the slightly larger in diameter of groove 3 is in the diameter that connects bacterial spawn 5, and the degree of depth of groove 3 is slightly less than the height that connects bacterial spawn 5.The side of frid 1 and cover plate 2 is provided with corresponding locating structure, shown in locating structure be junction of the edges of two sheets of paper groove 6, convenient connecing of cover plate 2 realized aiming at when bacterial spawn 5 is pressed into the groove 3 of frid 1.
The multiple spot of present embodiment connects the bacterium device, frid 1, cover plate 2 and cover plate are carried button 4 and are glass fiber reinforced polyester tetrafluoroethylene plate, connect bacterial spawn 5 and be the stainless steel cylinder, it is all high temperature material, can carry out repeatedly (121 ℃ of high pressure steam sterilizations in high-pressure steam sterilizing pan, 30min), can not deform and damage.
The multiple spot of present embodiment connects the bacterium device, during use, in advance bacterial strain to be measured is made bacteria suspension, and equivalent is clicked and entered in the groove 3 of autoclaved frid 1; The junction of the edges of two sheets of paper groove 6 of alignment frid 1 and cover plate 2, hand-held cover plate is carried button 4, will be on autoclaved cover plate 1 connect in the groove 3 that bacterial spawn 5 is pressed into frid 1 correspondence; Rear proposition is gently pressed the bacterial spawn 5 that connects on cover plate 1 on substratum, just with on the same substratum of multi-strain bacteria kind access, realizes that the single job multiple spot connects bacterium, the problem of the inoculation confusion that causes can not occur extracting because of the bacterial classification dislocation.
As a kind of improvement project of the present utility model, as shown in Fig. 5-7, described locating structure is the grafting locating structure, and described grafting locating structure is backstay 7 and the locating slot 8 of mutually pegging graft and coordinating.Three irregular positions at cover plate 2 sides arrange three backstays 7, and the direction of backstay 7 and length are with to connect bacterial spawn 5 consistent; Corresponding position arranges three locating slots 8 on frid 1.When this grafting locating structure had been determined connecing bacterial spawn 5 and be pressed into groove 3 on frid 1 on cover plate 2, the uniqueness of inserting mode when namely only having three backstay correspondences locating slot separately to insert, could realize the extraction of bacterial classification.The grafting locating structure that is made of backstay 7 and locating slot 8 makes cover plate and frid in the situation that dislocation can't be carried out bacterial classification extracts, and prevents because of carelessness connecing bacterial spawn and be pressed into just find it is the situation of the inoculation failure that causes of dislocation extraction after groove on frid on cover plate.
As the second improvement project of the present utility model, frid and cover plate can be square plate, rectangular slab or other erose plates.Groove on frid can be arranged mode, other rule or irregular arrangement modes anyhow, and the arrangement mode that connects bacterial spawn on cover plate is corresponding with the groove on frid.
Multiple spot of the present utility model connects the bacterium device, and frid and cover plate are provided with corresponding locating structure, when realizing that the single job multiple spot connects bacterium, guarantees that bacterial classification extracts accurately location, solves the problem of extracting the inoculation confusion that causes because of the bacterial classification dislocation; Simple to operate, quick, easy to use, save reagent and medicine, save a large amount of human and material resources; Connecing bacterial strain after bacterium can rule and be evenly distributed on substratum, is convenient to observed result.
Claims (10)
1. a multiple spot connects the bacterium device, comprise frid and the cover plate corresponding with frid, described frid is provided with at least 2 diameters columniform groove identical with the degree of depth, the lower surface of described cover plate is provided with at least 2 diameters and is fixedly connected with on the cover board the bacterial spawn that connects with highly identical and an end, describedly connect bacterial spawn grafting corresponding to groove on frid and coordinate, it is characterized in that: described frid and cover plate are provided with corresponding locating structure.
2. multiple spot according to claim 1 connects the bacterium device, it is characterized in that: described locating structure is the grafting locating structure, be located at junction of the edges of two sheets of paper groove or the roulette of frid and cover plate side.
3. multiple spot according to claim 2 connects the bacterium device, it is characterized in that: described grafting locating structure is by the groove that is located at respectively the irregular distribution on frid and cover plate and connect bacterial spawn and consist of.
4. multiple spot according to claim 2 connects the bacterium device, it is characterized in that: described grafting locating structure is made of backstay and the locating slot that mutual grafting coordinates.
5. multiple spot according to claim 1 connects the bacterium device, it is characterized in that: the upper surface of described cover plate is fixedly connected with cover plate and carries button.
6. multiple spot according to claim 5 connects the bacterium device, it is characterized in that: it is tabular that described cover plate is carried button.
7. multiple spot according to claim 6 connects the bacterium device, it is characterized in that: described cover plate is put forward level one side of button towards described locating structure.
8. multiple spot according to claim 1 connects the bacterium device, it is characterized in that: described frid and cover plate are disc-shaped plate.
9. multiple spot according to claim 1 connects the bacterium device, it is characterized in that: described frid and cover plate are glass fiber reinforced polyester tetrafluoroethylene plate.
10. multiple spot according to claim 1 connects the bacterium device, it is characterized in that: the described bacterial spawn that connects is the stainless steel cylinder.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2013200222440U CN203021562U (en) | 2013-01-16 | 2013-01-16 | Multipoint inoculation device |
Applications Claiming Priority (1)
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CN2013200222440U CN203021562U (en) | 2013-01-16 | 2013-01-16 | Multipoint inoculation device |
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CN203021562U true CN203021562U (en) | 2013-06-26 |
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CN2013200222440U Expired - Fee Related CN203021562U (en) | 2013-01-16 | 2013-01-16 | Multipoint inoculation device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104342363A (en) * | 2014-10-23 | 2015-02-11 | 沈阳师范大学 | Quick mass inoculator |
CN111345169A (en) * | 2020-02-25 | 2020-06-30 | 浙江省农业科学院 | Needle-punching type plant germ inoculation method and novel needle-punching type plant germ inoculation equipment |
-
2013
- 2013-01-16 CN CN2013200222440U patent/CN203021562U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104342363A (en) * | 2014-10-23 | 2015-02-11 | 沈阳师范大学 | Quick mass inoculator |
CN111345169A (en) * | 2020-02-25 | 2020-06-30 | 浙江省农业科学院 | Needle-punching type plant germ inoculation method and novel needle-punching type plant germ inoculation equipment |
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Legal Events
Date | Code | Title | Description |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130626 Termination date: 20190116 |