CN204224598U - Batch rapid inoculator - Google Patents
Batch rapid inoculator Download PDFInfo
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- CN204224598U CN204224598U CN201420616907.6U CN201420616907U CN204224598U CN 204224598 U CN204224598 U CN 204224598U CN 201420616907 U CN201420616907 U CN 201420616907U CN 204224598 U CN204224598 U CN 204224598U
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- metal
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- 239000002184 metal Substances 0.000 claims abstract description 58
- 229920003023 plastic Polymers 0.000 claims abstract description 46
- 238000011081 inoculation Methods 0.000 claims abstract description 42
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 238000000926 separation method Methods 0.000 claims 1
- 238000002255 vaccination Methods 0.000 claims 1
- 238000005192 partition Methods 0.000 abstract description 11
- 230000001580 bacterial effect Effects 0.000 abstract description 8
- 230000003750 conditioning effect Effects 0.000 abstract description 3
- 210000003813 thumb Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
批量快速接种器，包括塑料盖、金属接种盘和调菌盘三部分；金属接种盘由金属杆、金属圆盘和金属接种针组成，金属杆垂直固定在金属圆盘上表面中心位置，接种针固定在金属圆盘下表面，金属接种盘置于塑料盖内部，金属杆插入到塑料盖上端中空的塑料杆内，弹簧处于塑料杆内，其上端卡在金属杆上，下端处于塑料盖上，金属圆盘侧面的凸台嵌入塑料盖侧面凸台内，调菌盘内部分隔为多个独立的格子，接种器主体盖在调菌盘上，同时接种针位于与之对应的格子上方，不与调菌盘侧壁、隔板及盘底接触。使用本发明，一次操作即可完成多个菌液的接种，大大提高了工作效率。同时该接种器可被灭菌反复使用，节能环保。
Batch rapid inoculator, including three parts: plastic cover, metal inoculation tray and bacteria adjustment tray; the metal inoculation tray is composed of a metal rod, a metal disc and a metal inoculation needle, the metal rod is vertically fixed on the center of the upper surface of the metal disc, and the inoculation needle Fixed on the lower surface of the metal disc, the metal inoculation plate is placed inside the plastic cover, the metal rod is inserted into the hollow plastic rod at the upper end of the plastic cover, the spring is inside the plastic rod, the upper end is stuck on the metal rod, and the lower end is on the plastic cover. The convex platform on the side of the metal disc is embedded in the convex platform on the side of the plastic cover. The interior of the bacteria adjustment tray is divided into multiple independent grids. The main body of the inoculator is covered on the bacteria adjustment tray, and the inoculation needle is located above the corresponding grid. The side wall of the bacteria conditioning tray, the partition and the bottom of the tray are in contact. Using the invention, the inoculation of multiple bacterial solutions can be completed in one operation, which greatly improves the working efficiency. At the same time, the inoculator can be sterilized and used repeatedly, which is energy-saving and environment-friendly.
Description
技术领域：本实用新型是一种接种器，尤其是一种批量快速接种器。Technical field: the utility model is an inoculator, especially a batch rapid inoculator.
背景技术:在微生物实验工作中，通常会采用平板点种对大量菌株进行不同性质测定。通常测定1个性质需要取1次菌株，同时测定1个菌的多个性质时，需要重复多次取相同菌株，这会使工作繁琐，工作量增大。Background technology: in the microbiology experiment work, generally can adopt plate spot kind to carry out different property determination to a large number of bacterial strains. Usually, it is necessary to take one strain to measure one property. When measuring multiple properties of one bacteria at the same time, it is necessary to repeatedly take the same strain, which will make the work cumbersome and increase the workload.
实用新型内容：针对上述现有技术的不足，本实用新型提供了一种批量快速接种器。The content of the utility model: aiming at the deficiencies of the above-mentioned prior art, the utility model provides a batch rapid inoculator.
为实现上述目的，本实用新型采用的技术方案是：批量快速接种器，包括塑料盖、金属接种盘和调菌盘三部分。金属接种盘由金属杆、金属圆盘和金属接种针组成。金属杆垂直固定在金属圆盘上表面中心位置，接种针按一定排列固定在金属圆盘下表面。金属接种盘置于塑料盖内部，金属杆插入到塑料盖上端中空的塑料杆内，弹簧处于塑料杆内，其上端卡在金属杆上，下端处于塑料盖上，使金属接种盘可以在塑料盖内上下移动，金属圆盘侧面的凸台嵌入塑料盖侧面凸台内，使金属接种盘和塑料盖位置相对固定，二者组合成接种器主体。调菌盘为塑料圆盘，内部按一定排列分隔为多个独立的格子，接种器主体可盖在调菌盘上，同时接种针位于与之对应的格子上方，不与调菌盘侧壁、隔板及盘底接触。In order to achieve the above object, the technical solution adopted by the utility model is: a batch rapid inoculator, including three parts of a plastic cover, a metal inoculation tray and a bacteria adjustment tray. The metal inoculation tray consists of a metal rod, a metal disc and a metal inoculation needle. The metal rod is vertically fixed on the center of the upper surface of the metal disc, and the inoculation needles are fixed on the lower surface of the metal disc in a certain arrangement. The metal inoculation tray is placed inside the plastic cover, the metal rod is inserted into the hollow plastic rod at the upper end of the plastic cover, the spring is inside the plastic rod, the upper end is stuck on the metal rod, and the lower end is on the plastic cover, so that the metal inoculation tray can be placed on the plastic cover Moving up and down, the boss on the side of the metal disc is embedded in the boss on the side of the plastic cover, so that the position of the metal inoculation tray and the plastic cover are relatively fixed, and the two are combined to form the main body of the inoculator. The bacteria-regulating tray is a plastic disc, and the interior is divided into multiple independent grids according to a certain arrangement. The main body of the inoculator can be covered on the bacteria-adjusting tray, and the inoculation needle is located above the corresponding grid. The partition and the bottom of the plate are in contact.
本实用新型使用时先将每个菌液置于调菌盘各格子中，再操作接种器主体，用接种针蘸取调菌盘格子内菌液，然后将其接种到培养基上，反复沾取菌液可以接种到多个培养基上。使用本实用新型，一次操作即可完成多个菌液的接种，大大提高了工作效率。同时该接种器可被灭菌反复使用，节能环保。When the utility model is in use, first place each bacterial solution in each grid of the bacteria-adjusting tray, then operate the main body of the inoculator, use an inoculation needle to dip the bacterial solution in the grid of the bacteria-adjusting tray, and then inoculate it on the culture medium, dip it repeatedly The bacterial solution can be inoculated onto multiple culture media. With the utility model, the inoculation of multiple bacterial liquids can be completed in one operation, which greatly improves the work efficiency. At the same time, the inoculator can be sterilized and used repeatedly, which is energy-saving and environment-friendly.
附图说明Description of drawings
图1是本实用新型的剖视图。Fig. 1 is a sectional view of the utility model.
图2是本实用新型的外形图。Fig. 2 is an outline view of the utility model.
图3是金属接种盘的结构示意图。Fig. 3 is a schematic diagram of the structure of the metal inoculation tray.
图4是调菌盘的结构示意图。Fig. 4 is a schematic diagram of the structure of the bacteria adjustment tray.
图5是塑料盖的结构示意图。Fig. 5 is a structural schematic diagram of the plastic cover.
图中：1-金属杆 2-塑料杆 3-弹簧 4-塑料盖 5-金属圆盘 6-接种针 7-调菌盘 8-凸台 9-隔板In the figure: 1-metal rod 2-plastic rod 3-spring 4-plastic cover 5-metal disc 6-inoculation needle 7-bacteria adjustment plate 8-boss 9-partition
具体实施方式：Detailed ways:
下面将结合本实用新型实施例中的附图，对本实用新型实施例中的技术方案进行清晰、完整地描述，显然，所描述的实施例仅仅是本实用新型一部分实施例，而不是全部的实施例。基于本实用新型中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本实用新型保护的范围。The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.
如图1-图5所示：批量快速接种器，包括塑料盖4、金属接种盘和调菌盘7。金属接种盘由金属杆1、金属圆盘5和接种针6组成。金属杆1垂直固定在金属圆盘5上表面中心位置，接种针6固定在金属圆盘5的下表面。金属杆1插入到塑料盖4上端中空的塑料杆2内。金属圆盘5侧面的凸台嵌入塑料盖4侧面凸台8内。塑料杆2内设有弹簧3，弹簧3上端卡在金属杆1上，下端处于塑料盖4上。塑料盖4盖在调菌盘7上。接种针6处于调菌盘7的上方。调菌盘7内由隔板9分隔为多个独立的格子。使用时，拇指按压超出塑料壳外的金属杆顶端，使金属圆盘下端的接种针接触调菌盘，沾取菌液。As shown in Figures 1-5: a batch rapid inoculator, including a plastic cover 4, a metal inoculation tray and a bacteria adjustment tray 7. The metal inoculation tray consists of a metal rod 1, a metal disc 5 and an inoculation needle 6. The metal rod 1 is vertically fixed on the center position of the upper surface of the metal disc 5 , and the inoculation needle 6 is fixed on the lower surface of the metal disc 5 . The metal rod 1 is inserted into the hollow plastic rod 2 at the upper end of the plastic cover 4 . The bosses on the sides of the metal disc 5 are embedded in the bosses 8 on the sides of the plastic cover 4 . A spring 3 is arranged in the plastic rod 2 , the upper end of the spring 3 is stuck on the metal rod 1 , and the lower end is on the plastic cover 4 . Plastic cover 4 is covered on the bacterium conditioning dish 7. The inoculation needle 6 is above the bacterium adjustment tray 7 . The bacteria-adjusting tray 7 is divided into a plurality of independent grids by a partition 9 . When in use, the thumb presses the top of the metal rod protruding from the plastic shell, so that the inoculation needle at the lower end of the metal disc touches the bacteria regulating plate, and the bacteria liquid is dipped.
各部分参数如下：The parameters of each part are as follows:
A.塑料盖：A. Plastic cover:
1.透明塑料耐高温圆盘盖，直径95mm，外沿高16mm，壁厚1mm(培养皿直径90mm，外沿高18mm，其能与培养皿很好吻合)。1. Transparent plastic heat-resistant disc cover, diameter 95mm, outer edge height 16mm, wall thickness 1mm (petri dish diameter 90mm, outer edge height 18mm, which can fit well with the culture dish).
2.盖中心有一个8mm直径圆孔(该直径尺寸保证支撑弹簧不至于脱落)。2. There is a round hole with a diameter of 8mm in the center of the cover (this diameter ensures that the support spring will not fall off).
3.圆盘盖外沿有一凸台。3. There is a boss on the outer edge of the disc cover.
4.盖体中心连接一中空圆柱杆，高85mm，外壁直径16mm(该尺寸适宜手掌拿握)。4. A hollow cylindrical rod is connected to the center of the cover body, with a height of 85mm and an outer wall diameter of 16mm (this size is suitable for holding in the palm of the hand).
5.手柄内中空部分直径7mm，并设有两个相对的长2mm，宽3mm，高55mm凹槽，与金属杆凸起形状吻合。5. The diameter of the hollow part inside the handle is 7mm, and there are two opposite grooves with a length of 2mm, a width of 3mm, and a height of 55mm, which match the shape of the metal rod protrusion.
6.距手柄底端长30mm为直径14mm中空圆形。6. The length 30mm from the bottom of the handle is a hollow circle with a diameter of 14mm.
B.金属接种盘：B. Metal Inoculation Tray:
1.金属圆盘直径84mm，厚1mm(其直径应适当小于培养皿直径90mm，使其不能接触到培养皿侧沿)。1. The diameter of the metal disc is 84mm, and the thickness is 1mm (the diameter should be appropriately smaller than the diameter of the petri dish, which is 90mm, so that it cannot touch the side edge of the petri dish).
2.金属圆盘边缘有一凸台，与塑料盖凸台吻合。2. There is a boss on the edge of the metal disc, which coincides with the boss of the plastic cover.
3.金属圆盘中心处由螺丝连接1金属杆，螺丝直径3mm，厚1mm(其厚度不能超过2mm，否则会接触到调菌盘内分隔板)。3. The center of the metal disc is connected with a metal rod by a screw. The diameter of the screw is 3mm and the thickness is 1mm (the thickness cannot exceed 2mm, otherwise it will touch the partition plate in the bacteria conditioning tray).
4.金属杆主体为长100mm，直径6mm的圆柱体(该长度使其能有足够的压缩弹簧向下移动空间)。4. The main body of the metal rod is a cylinder with a length of 100mm and a diameter of 6mm (this length allows enough space for the compressed spring to move downward).
5.金属杆上部设有两个相对的宽2mm，长2mm，高70mm金属长方体凸起。5. The upper part of the metal rod is provided with two opposite metal cuboid protrusions with a width of 2mm, a length of 2mm, and a height of 70mm.
6.金属杆下端套一30mm高弹簧(该长度能够保证弹簧向下移动距离达到10mm，其为针尖接触到培养皿底部的最大距离)。6. The lower end of the metal rod is covered with a 30mm high spring (this length can ensure that the spring moves down to 10mm, which is the maximum distance from the needle tip to the bottom of the culture dish).
C.接种针布局：C. Inoculation Needle Layout:
1.接种针共32根，按6列排布。1. A total of 32 inoculation needles are arranged in 6 columns.
2.每列依次为4、6、6、6、6、4根针(这样排布可以保证每个针接种后，生长的菌周围有10mm的反应空间，菌之间的现象互不干扰，便于观察结果)。2. There are 4, 6, 6, 6, 6, and 4 needles in each row (this arrangement can ensure that after each needle is inoculated, there is a 10mm reaction space around the growing bacteria, and the phenomena between the bacteria do not interfere with each other. to observe the results).
3.金属圆盘上凸台位于第3、4列针中线上，操作时，该凸台始终处于同一方向，用于定位。3. The boss on the metal disc is located on the center line of the needles in the 3rd and 4th columns. During operation, the boss is always in the same direction for positioning.
4.接种针直径1mm，长8mm(此长度既保证针的下端不超过塑料盖外沿高度，又能避免塑料盖盖到调菌盘上时，针尖触碰到调菌盘内的隔板，造成污染)。4. The diameter of the inoculation needle is 1mm, and the length is 8mm (this length not only ensures that the lower end of the needle does not exceed the height of the outer edge of the plastic cover, but also prevents the needle tip from touching the partition in the bacteria-adjusting tray when the plastic cover is placed on the bacteria-adjusting tray. create pollution).
5.相邻接种针间距14mm(该距离既保证处于金属板边缘的针在接种时不能碰到培养皿侧壁，又保证接种后每个菌之间有足够的生长空间)。5. The distance between adjacent inoculation needles is 14mm (this distance not only ensures that the needles at the edge of the metal plate cannot touch the side wall of the culture dish during inoculation, but also ensures that there is enough growth space between each bacteria after inoculation).
D.调菌盘；D. Sterilization plate;
1.调菌盘部分是由耐高温塑料制成的90mm圆盘，外沿高18mm，壁厚1mm。(保证塑料盖盖上后，边缘不与试验台面接触)1. The bacteria-adjusting tray part is a 90mm disc made of high-temperature-resistant plastic, with an outer edge height of 18mm and a wall thickness of 1mm. (Ensure that the edge of the plastic cover is not in contact with the test bench surface)
2.圆盘外沿与盖子对应处设有一凸台，长6mm，宽3mm，高18mm，以使其与塑料盖凸台吻合。2. There is a boss on the outer edge of the disc corresponding to the cover, with a length of 6mm, a width of 3mm, and a height of 18mm, so that it matches the boss of the plastic cover.
3.盘内由塑料隔板分隔成32个格子，隔板厚1mm，高6mm。(隔板的高度要小于接种针的长度，以免触碰污染；隔板高度也不能过低，会导致每个接种格子内的容积过小)3. The inside of the tray is divided into 32 grids by plastic partitions, the thickness of the partitions is 1mm, and the height is 6mm. (The height of the partition should be less than the length of the inoculation needle to avoid contamination; the height of the partition should not be too low, which will cause the volume of each inoculation grid to be too small)
4.圆盘直径线上有一隔板，圆心起上下左右平均分布边长15mm的正方形小格16个，余下与圆盘周围侧壁相交空间处各分布4个小格，共计32格。4. There is a partition on the diameter line of the disc. From the center of the circle, there are 16 small squares with a side length of 15mm evenly distributed up, down, left, and right, and 4 small grids are distributed in the remaining spaces where the side walls intersect with the surrounding discs, totaling 32 grids.
使用方法：Instructions:
将菌液加入格子内，1格1菌。在纸上记好每孔样品名称。然后接种器取菌液：握住外部塑料杆，对应好凸台的位置，将上部接种器盖在调菌盘上。拇指按压顶部金属杆，使接种针接触调菌盘内菌液，反复按压可使针尖均匀沾取菌液。松开拇指，将沾取菌液的接种器移到实验用平皿上，预先在平皿外底部用笔做好方向标记，此时调整凸台，使其与平皿底部标记指向相同方向，便于记录每个菌接种的位置。用拇指按压金属杆，使接种针尖接触到培养基表面，接种完成。重复同法沾取菌液可一次性完成批量接种任务。Add the bacteria solution into the grid, 1 bacteria per grid. Write down the sample name for each well on the paper. Then the inoculator takes the bacteria solution: hold the outer plastic rod, correspond to the position of the boss, and cover the upper inoculator on the bacteria adjustment tray. Press the metal rod on the top with your thumb to make the inoculation needle touch the bacteria solution in the bacteria-adjusting tray, and press repeatedly to make the needle tip evenly dip in the bacteria solution. Release the thumb, move the inoculator dipped in the bacterial solution to the experimental plate, and mark the direction with a pen on the outer bottom of the plate in advance. The location of the bacteria inoculation. Press the metal rod with your thumb so that the tip of the inoculation needle touches the surface of the medium, and the inoculation is complete. Batch inoculation tasks can be completed at one time by repeating the same method to pick up the bacterial solution.
Claims (6)
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| CN201420616907.6U CN204224598U (en) | 2014-10-23 | 2014-10-23 | Batch rapid inoculator |
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| CN201420616907.6U CN204224598U (en) | 2014-10-23 | 2014-10-23 | Batch rapid inoculator |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104342363A (en) * | 2014-10-23 | 2015-02-11 | 沈阳师范大学 | Quick mass inoculator |
| CN105255715A (en) * | 2015-11-17 | 2016-01-20 | 河南省农业科学院园艺研究所 | High-pass multipurpose controllable microorganism inoculation device and batch strain inoculation method |
-
2014
- 2014-10-23 CN CN201420616907.6U patent/CN204224598U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104342363A (en) * | 2014-10-23 | 2015-02-11 | 沈阳师范大学 | Quick mass inoculator |
| CN105255715A (en) * | 2015-11-17 | 2016-01-20 | 河南省农业科学院园艺研究所 | High-pass multipurpose controllable microorganism inoculation device and batch strain inoculation method |
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Granted publication date: 20150325 Effective date of abandoning: 20160824 |
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