CN111345169A - Needle-punching type plant germ inoculation method and novel needle-punching type plant germ inoculation equipment - Google Patents
Needle-punching type plant germ inoculation method and novel needle-punching type plant germ inoculation equipment Download PDFInfo
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- CN111345169A CN111345169A CN202010115210.0A CN202010115210A CN111345169A CN 111345169 A CN111345169 A CN 111345169A CN 202010115210 A CN202010115210 A CN 202010115210A CN 111345169 A CN111345169 A CN 111345169A
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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Abstract
The invention relates to a needle-punching type plant germ inoculation method, wherein a plurality of inoculation needles are fixed on an inoculation needle disk provided with a hand grip structure, the inoculation needles are attached with bacteria liquid, the hand grip structure is held to move all the inoculation needles to pierce through leaves or stems of plants to form puncture holes, and the bacteria liquid is transferred into the puncture holes when the inoculation needles form the puncture holes to complete inoculation. Still relate to a novel acupuncture formula plant germ inoculation equipment, including inoculation dial, one side of inoculation dial is equipped with tongs structure, the opposite side rigid coupling has a plurality of inoculation needles, and inoculation dial and inoculation needle protection casing enclose into inoculation needle protection chamber, state the inoculation needle and be located inoculation needle protection intracavity, are equipped with the clearance between inoculation needle and the inoculation needle protection casing when inoculation dial supports on the stopper. The invention has the advantages of preventing the position change between the inoculating needles for inoculating and conveniently protecting the inoculating needles, and solves the problems that the position change of the inoculating needles is easy to generate, the inoculating needles are inconvenient to hold and the protecting effect of the inoculating needles is poor.
Description
Technical Field
The invention relates to the technical field of plant germ inoculation, in particular to a needle-punching type plant germ inoculation method and novel needle-punching type plant germ inoculation equipment.
Background
The needle-punching method is a method commonly used in plant disease inoculation experiments, namely, a needle is used for dipping bacteria liquid and then puncturing plant bodies (leaves and stems) or a syringe is used for sucking the bacteria liquid and injecting the bacteria liquid into the plant bodies through the needle head, so that pathogenic bacteria directly enter plant tissues. For example, bacterial leaf streak of rice is successfully inoculated by dipping a germ suspension liquid with a needle and continuously puncturing two times at the same position in the middle of the tip of a leaf from the seedling stage to the heading stage of the rice. Hereinafter, the needle and the needle head used for inoculating plant germs are collectively referred to as an inoculating needle.
The following disadvantages exist when the splicing is carried out at present: when inoculating, a plurality of inoculating needles are needed to simultaneously puncture a plant body, and the existing plurality of inoculating needles are tied together for operation, so that the consistency of the inoculating position is poor due to easy looseness; the operator has great difficulty in holding (or pinching) the inoculating needle, which causes inconvenience in inoculating; the lack of a needle tip protection device on the inoculating needle can cause the inoculating needle tip to be polluted by other mixed bacteria to cause test errors or mistakes; the puncture can not be accurately performed at the position of the primary puncture during the secondary puncture; it is not possible to easily pierce the inoculating needle into the plant body.
Disclosure of Invention
The invention provides a needle-type plant germ inoculation method capable of preventing position change among inoculation needles for inoculation and conveniently protecting the inoculation needles, and solves the problems that position change is easy to generate in inoculation when bacteria inoculation is bound together, inconvenience is caused when the inoculation needles are held, and the protection effect of the inoculation needles is poor.
The invention also provides novel needling type plant germ inoculation equipment which can prevent the position change between the inoculation needles for inoculation and can conveniently protect the inoculation needles, and solves the problems that the position change is easy to generate when the inoculation needles are bound together for inoculation, the inoculation needles are inconvenient to hold and the protection effect of the inoculation needles is poor.
The invention further provides novel needling type plant pathogen inoculation equipment capable of conveniently puncturing plant bodies, and the problem that the existing inoculation equipment cannot conveniently puncture the plant bodies is solved.
The invention further provides novel needling type plant germ inoculation equipment with accurately superposed secondary puncture positions, and the problem that the existing inoculation equipment cannot accurately puncture the primary puncture position during secondary puncture is solved.
The technical problem is solved by the following technical scheme: a needle-punching type inoculation method for plant germs comprises the steps of fixing a plurality of inoculation needles on an inoculation needle disk with a hand grip structure, enabling the inoculation needles to attach bacteria liquid, holding the hand grip structure to move all the inoculation needles to pierce leaves or stems of plants to form puncture holes, and transferring the bacteria liquid into the puncture holes when the inoculation needles form the puncture holes to complete inoculation. By securing the inoculating needles to the inoculating tray, relative movement between the inoculating needles is prevented. The inoculating needle disk is moved by arranging the gripper structure, so that the inoculating needle can be moved simultaneously, and the inoculating needle is convenient to move.
The utility model provides a novel acupuncture formula plant germ inoculation equipment, includes inoculation needle and inoculation dial, one side of inoculation dial is equipped with tongs structure, opposite side rigid coupling has a plurality of the inoculation needle, the inoculation dial is worn to establish in the inoculation needle protection casing, inoculation dial and inoculation needle protection casing enclose into inoculation needle protection chamber, the inoculation needle is located inoculation needle protection intracavity, be equipped with the stopper on the inner peripheral surface of inoculation needle protection casing, the inoculation dial supports when on the stopper the inoculation needle with be equipped with the clearance between the inoculation needle protection casing. Move the inoculation needle when so realizing the inoculation needle of inoculation dial through setting up tongs structure, it is convenient when removing the inoculation needle, set up the protection casing and protect for the inoculation needle is difficult to the pollution. The meaning of the different protective covers contacting each other when the inoculating needle is in the protective cover is that a gap is arranged between the inoculating needle and the protective cover of the inoculating needle.
Preferably, the grip structure comprises a holding block and a connecting handle, one end of the connecting handle is connected with the holding block, and the other end of the connecting handle is connected to one side, away from the inoculating needle, of the inoculating plate. When in use, the gripping block is gripped to realize gripping of the invention. The gripper has the advantages of simple structure and convenience in use.
Preferably, the connecting handle is of a hollow structure. Light weight and labor saving when in use.
Preferably, the inoculating needle protective cover comprises a tube body and an end plate, the end plate is provided with a skirt edge, the end plate is in threaded connection with the tube body through the skirt edge and is detachably connected with the tube body, the inoculating plate is supported on the limiting block through a compression spring, the needle tip of the inoculating needle is completely accommodated in the tube body, and the needle tip of the inoculating needle extends out of the tube body when the inoculating plate is pressed towards the limiting block. When inoculating, take off the end plate, then align with the one end that the body is connected with the end plate and press the plant body, grasp the tongs structure and press the crimping dish towards the stopper and make compression spring shrink, the inoculation needle stretches out and punctures the plant body from the one end that the body is connected with the end plate to accomplish the inoculation. Can improve the convenience when puncturing the plant body. The protection casing can be fixed to the plant object when the puncture, the function of the protection casing of expansion.
Preferably, the skirt is screwed to the inner circumferential surface of the pipe body. The flatness of the outer surface of the shield can be maintained.
Preferably, the skirt edge is provided with a leaf inlet and outlet hole for the leaves of the inoculated plant to enter and exit; when the skirt edge rotates towards the interior of the tube body to the limit position, the blade inlet and outlet holes are completely shielded by the tube body; the inoculating tray is pressed to the limit position towards the direction of the limiting block when the blade inlet and outlet holes are exposed outside the tube body, and a gap is formed between the needle tip of the inoculating needle and the end plate. When the inoculating needle is protected, the blade inlet and outlet holes are completely sealed by the tube body; when the stalks with the leaves are punctured, the end plate is taken down and the stalks are punctured by pressing the end plate through the tube body; when the blades are punctured, the blade inlet and outlet holes are in an exposed state, the blades are inserted into the protective cover through the blade inlet and outlet holes, and then the inoculation dial is pressed to move towards the limiting block, so that the inoculation is punctured for the blades. The blade has good reliability when being punctured.
Preferably, the inner surface of the end plate is provided with an elastic supporting pad for supporting the blades penetrating into the skirt edge through the blade inlet and outlet holes, and the elastic supporting pad is provided with an inoculation needle avoiding hole aligned with the inoculation needle. Can reduce the extrusion damage to the blade during puncture.
Preferably, the inoculating needles are distributed along the circumferential direction of the end plate, and the inoculating needle avoiding holes are annular and extend along the circumferential direction of the end plate. Can make the end plate no matter when rotating to what angle, the inoculation needle can all dodge the hole with the inoculation needle and align. The convenience is good when in use.
Preferably, the hand in the gripper structure is provided with a slide hole extending along the axial direction of the inoculating needle disc, the slide hole is provided with an avoiding through groove and a limiting groove extending along the extension direction of the slide hole, a first piston and a second piston are arranged in the slide hole, a liquid storage cavity and a liquid supplementing cavity are separated from each other in the slide hole by the first piston and the second piston, the peripheral surface of the inoculating needle is provided with a bacteria liquid output port, the liquid storage cavity is communicated with the bacteria liquid conveying port through a bacteria liquid conveying channel, the first piston is connected with the inoculating needle protective cover through a fixing rod penetrating through the avoiding through groove, bacteria liquid and air are stored in the first bacteria liquid storage cavity, bacteria liquid is stored in the liquid supplementing cavity, the second piston is connected with a push rod extending out of the slide hole, and the first piston is provided with a limiting protrusion positioned in the limiting groove and a piston part channel communicating the end surface of the limiting protrusion with the, a check valve which is opened towards the liquid storage cavity is arranged in the piston part channel, a transfer cavity is arranged in the gripper structure, the transfer cavity is provided with a first communicating hole and a second communicating hole which are communicated with the sliding hole, a plug for sealing the first communicating hole and a plug ejecting spring for driving the plug to extend out of the first communicating hole and protrude out of the limiting groove, and a plug part channel for communicating the end face of the plug and the transfer cavity is arranged in the plug; when the inoculating needle disc moves to the position where the fixed rod is abutted with the end wall of the avoiding through groove in the direction away from the limiting block, the plug is abutted with the limiting protrusion, the piston part channel is communicated with the plug part channel, and the second communication hole is communicated with the transfer cavity and the liquid supplementing cavity. When the device is used, after the bacterial liquid in the liquid storage cavity flows out from the bacterial liquid output port and reaches the surface of the inoculating needle to reach the preset volume, the inoculating needle disc is pressed towards the limiting block to enable the inoculating needle disc to puncture plants, the volume of the liquid storage cavity is increased in the process of pressing the inoculating needle disc to form negative pressure, the bacterial liquid in the bacterial liquid conveying channel flows towards the liquid storage cavity to prevent the bacterial liquid from flowing out from the bacterial liquid output port to cause the bacterial liquid to be too much, and the device plays a role in saving the bacterial liquid consumption. After the puncture is finished, the pressing action on the inoculating needle disc is released, so that the inoculating needle is reset to be blocked on the end face of the avoiding through groove by the fixed rod under the action of the compression spring and stops moving, the plug is abutted with the limiting protrusion, the piston part channel is communicated with the plug part channel, and the second communicating hole is communicated with the transfer cavity and the liquid supplementing cavity; the fungus liquid in the fluid infusion chamber reaches the liquid storage chamber through the second communicating hole, the transfer chamber, the plug part channel, the piston part channel and the one-way valve under the action of the pressure difference to increase the pressure in the liquid storage chamber, so that the fungus liquid in the liquid storage chamber is output from the fungus liquid output port, and meanwhile, an experimenter observes the conditions of the plants. If the amount of the bacterial liquid flowing out of the bacterial liquid outlet onto the inoculating needle is too small when the secondary puncture is needed after the observation is finished, the push rod drives the second piston to move towards the first piston so as to accelerate the bacterial liquid to flow out of the surface of the inoculating needle. And after the bacteria liquid meets the requirements, pressing the inoculating needle disk towards the limiting block again to perform secondary puncture, so that the puncture of one plant body is completed. The next plant was inoculated as described above. For the purpose of observation, the protective cover is designed as a transparent structure. This technical scheme has realized that the inoculation needle need be got rid of when carrying out the secondary inoculation and has dipped in the fungus liquid to perforation when making the secondary inoculation can coincide completely, reliability when having improved the inoculation use. The bacterial liquid can flow onto the inoculating needle without manual operation during observation, so that labor is saved when the bacterial liquid is transferred onto the inoculating needle.
Preferably, the circumferential surface of the second piston is provided with jacks, the wall surface of the sliding hole is provided with a bolt hole, a bolt inserted into the jack to fix the second piston and the sliding hole together and a bolt ejection spring for driving the bolt to extend out of the bolt hole are arranged in the bolt hole in a penetrating manner, and the bolt is provided with a guide surface for guiding the bolt to be inserted into the jack and pulling out the jack; when the plug and the limiting protrusion are aligned, the bolt hole is located in one side, far away from the first piston, of the second piston. If observe the completion and need carry out before the secondary puncture the fungus liquid measure that flows out on the inoculating needle through the fungus liquid delivery outlet enough, then remove the second piston towards the direction of keeping away from first piston and align with the jack to the bolt and insert in the jack for the second piston can not remove, and then the stock solution chamber can not be popular to the fungus liquid of fluid infusion intracavity, and the fungus liquid of stock solution intracavity can not flow through the fungus liquid delivery outlet. This technical scheme can avoid the fungus liquid transition to flow out and lead to the waste.
The invention has the following advantages: the manufactured computer can automatically add grease into the bearing when the cooling fan works, so that labor is saved when the grease is added; when the cooling fan does not rotate, lubricating grease can not be added, so that the waste of the lubricating grease is avoided.
Drawings
FIG. 1 is a schematic view of a novel needle-prick type plant pathogen inoculation device in a first embodiment.
FIG. 2 is a schematic view of the novel needle-prick type plant pathogen inoculation apparatus in the second embodiment.
Fig. 3 is a partially enlarged schematic view of a portion a of fig. 2.
Fig. 4 is a partially enlarged schematic view of fig. 2 at B.
Fig. 5 is a partially enlarged schematic view of fig. 4 at C.
In the figure: the inoculation needle comprises an inoculation needle disc 1, an inoculation needle 2, an inoculation needle protective cover 3, a tube body 4, an end plate 5, an inoculation needle protective cavity 6, a limiting block 7, a holding block 8, a connecting handle 9, a sliding hole 10, a skirt 11, a compression spring 12, a blade access hole 13, an elastic supporting pad 14, an inoculation needle avoiding hole 15, an avoiding through groove 16, a limiting groove 17, a first piston 18, a second piston 19, a liquid storage cavity 20, a liquid supplementing cavity 21, a bacterial liquid output port 22, an inner cavity 23, a liquid outlet channel 24, a bacterial liquid conveying channel 25, a fixing rod 26, a push rod 27, a limiting bulge 28, a piston part channel 29, a one-way valve 30, a transfer cavity 31, a first communicating hole 42, a second communicating hole 32, a plug 33, an ejection spring 34, a plug part channel 35, a plug hole 36, a plug hole 37, a plug 38, a plug ejection spring 39, a guide surface 40.
Detailed Description
The invention is further described with reference to the following figures and examples.
A needle-prick type plant pathogen inoculation method is achieved by, but not limited to, the novel needle-prick type plant pathogen inoculation device disclosed by the invention.
Referring to fig. 1, in a first embodiment, a novel needle-prick type plant pathogen inoculation device comprises an inoculation needle plate 1. One side of the inoculation dial is provided with a hand grip structure, and the other side is fixedly connected with a plurality of inoculation needles 2 (specifically 6 inoculation needles). The inoculation needle disk penetrates through the inoculation needle protective cover 3. The inoculating needle shield comprises a tubular body 4 and an end plate 5. The tubular body 4 and the end plate 5 are integrally formed together. The inoculating needle disk and the inoculating needle protective cover enclose an inoculating needle protective cavity 6. The inoculating needle is positioned in the inoculating needle protective cavity. The inner circumferential surface of the inoculating needle protective cover is provided with a limiting block 7. The inoculating needle disc is supported on the limiting block and is contained in the protective cover. When the inoculating needle disc is supported on the limiting block, a gap is arranged between the inoculating needle and the inoculating needle protective cover, namely the inoculating needle is not contacted. The hand grip structure comprises a holding block 8 and a connecting handle 9 with one end connected with the holding block. The holding block is in the original shape. The other end of the connecting handle is connected to one side of the inoculation plate far away from the inoculation needle. The stem is hollow so as to form a slide hole 10 in the stem.
The inoculation method by the novel needle-prick type plant germ inoculation equipment comprises the following steps: the holding block is held to take out the inoculating needle disc from the inoculating needle protective cover, the inoculating needle disc is taken out, the inoculating needle is dipped in the bacterial liquid and is adhered to the bacterial liquid, secondary puncture is carried out on the same part of the plant body through inoculation (namely, the two punctures puncture the same point on the plant body (the best inoculating effect is achieved by complete overlapping), so that inoculation is completed, and the inoculating needle disc is placed back into the inoculating needle protective cover after inoculation is completed.
The second embodiment is different from the first embodiment in that:
referring to fig. 2, 3 and 4, the end plate is provided with a skirt 11. The outer surface of the skirt edge is provided with an external thread section. The inner surface of the lower end of the pipe body is provided with an internal thread section. The end plate is connected in the pipe body through the skirt thread and is detachably connected with the pipe body. The inoculating tray is supported on the limiting block through a compression spring 12 and the needle tip of the inoculating needle is completely accommodated in the tube body. The skirt edge is provided with a leaf inlet and outlet hole 13 for the leaves of the inoculated plants to enter and exit. When the skirt edge rotates towards the interior of the tube body to the limit position, the blade inlet and outlet holes are completely shielded by the tube body. When the blade inlet and outlet holes are exposed outside the tube body (namely in the state shown in figure 1), the seed plate is pressed to the limit position (namely the position where the compression spring can not continuously contract) towards the direction of the limit block, a gap is arranged between the needle tip of the inoculating needle and the end plate, and the needle tip of the inoculating needle extends out of the tube body. The inner surface of the end plate is provided with an elastic supporting pad 14 for supporting the blades which penetrate into the skirt edge through the blade inlet and outlet holes. The elastic supporting cushion is provided with an inoculation needle avoiding hole 15 aligned with the inoculation needle. The inoculating needles are distributed along the circumference of the end plate. The inoculation needle dodges the hole and is the annular that extends along the end plate circumference.
The slide hole 10 is provided with an avoidance through groove 16 and a limit groove 17 extending in the slide hole extending direction, i.e., the up-down direction. A first piston 18 and a second piston 19 are provided in the slide hole. The first piston and the second piston separate a liquid storage cavity 20 and a liquid supplementing cavity 21 in the slide hole. The circumferential surface of the inoculating needle is provided with a plurality of bacteria liquid output ports 22 which are distributed along the circumferential direction of the inoculating needle. An inner cavity 23 is arranged in the inoculating needle. The bacteria liquid output port is communicated with the inner cavity through the liquid outlet channel 24. The inner cavity is communicated with the liquid storage cavity through a bacterium liquid conveying channel 25. The first piston is connected to the body of the inoculating needle guard by a fixing rod 26 which passes through the escape channel. The fixed tube slidably seals through the second piston. The first bacteria liquid storing cavity is stored with bacteria liquid 41 and air (when in use, the liquid storing cavity can not be filled with bacteria liquid, needs to be opened, the bacteria liquid is stored in the liquid supplementing cavity, the second piston is connected with a push rod 27 extending out of the slide hole through the holding block, the first piston is provided with a limit bulge 28 positioned in the limit groove, an end surface of the limit bulge and a piston part channel 29 of the liquid storing cavity, the piston part channel is provided with a one-way valve 30 opening towards the liquid storing cavity, the connecting handle of the gripper structure is provided with a transfer cavity 31, the transfer cavity is provided with a first connecting hole 42 communicating with the slide hole, a second connecting hole 32, a plug 33 closing the first connecting hole and a plug ejecting spring 34 driving the plug to extend out of the first connecting hole and protrude out of the limit groove, the first connecting hole and the second connecting hole are both opened from the limit groove and communicated with the slide hole, the end surface of the plug and the plug part channel 35 of the transfer cavity are arranged in the plug, the inoculating needle When the lower end walls of the through grooves are abutted together, the plugs and the limiting protrusions are abutted together. The piston part channel is communicated with the plug part channel, and the second communication hole is communicated with the transfer cavity and the liquid replenishing cavity. The second piston is provided with insertion holes 36 on its circumferential surface. The wall surface of the slide hole is provided with a bolt hole 37. A bolt 38 for inserting into the insertion hole to fix the second piston with the slide hole and a bolt ejecting spring 39 for driving the bolt to extend out of the insertion hole are provided in the insertion hole. The pin is provided with a guide surface 40 which guides the pin into and out of the socket. When end cap and spacing arch align, the bolt hole is located the second piston and keeps away from one side of first piston and is the top, and when the fungus liquid has been injected into in fluid infusion chamber and the stock solution intracavity, the fungus liquid can not float second piston all with bolt hole alignment or be located the top in bolt hole. In this embodiment, the shield is a transparent structure.
In the embodiment, when the inoculating needle is protected, the blade inlet and outlet holes are completely sealed by the pipe body; when the stalks with the leaves are punctured, the end plate is taken down, and the plant body is pressed by the tube body to puncture; when the blades are punctured, the blade inlet and outlet holes are in an exposed state, the blades are inserted into the protective cover through the blade inlet and outlet holes, and then the inoculation dial is pressed to move towards the limiting block, so that the inoculation is punctured for the blades.
After the bacterial liquid in the liquid storage cavity flows out from the bacterial liquid output port and reaches the surface of the inoculating needle to reach the preset volume, the inoculating needle disc is pressed downwards towards the limiting block to enable the inoculating needle disc to puncture the plant body, the volume of the liquid storage cavity is increased in the process of pressing the inoculating needle disc to form negative pressure, the bacterial liquid in the inoculating needle and the bacterial liquid conveying channel flows towards the liquid storage cavity, the bacterial liquid is prevented from further flowing out from the bacterial liquid output port to cause the bacterial liquid to be too much, and the bacterial liquid consumption is saved. When the pressing action on the inoculating needle disc is released after the puncture is finished, the inoculating needle is reset to the position blocked on the end face of the avoiding through groove by the fixed rod under the action of the compression spring and stops moving, at the moment, the plug is abutted with the limiting protrusion, the piston part channel is communicated with the plug part channel, and the second communicating hole is communicated with the transfer cavity and the liquid supplementing cavity; the fungus liquid in the fluid infusion chamber reaches the liquid storage chamber through the second communicating hole, the transfer chamber, the plug part channel, the piston part channel and the one-way valve under the action of the pressure difference to increase the pressure in the liquid storage chamber, so that the fungus liquid in the liquid storage chamber is output from the fungus liquid output port, and meanwhile, an experimenter observes the conditions of the plants. If the amount of the bacterial liquid flowing out of the bacterial liquid outlet onto the inoculating needle is too small when secondary puncture is needed after observation is finished, the second piston is driven by the push rod to move towards the first piston so as to accelerate the bacterial liquid to flow out of the surface of the inoculating needle; if observe the completion and need carry out the fungus liquid measure that flows out to the inoculating needle through the fungus liquid delivery outlet before the secondary puncture had enough, then move the second piston up and align with the jack and insert in the jack to the bolt for the second piston can not remove, and then the stock solution chamber can not be popular to the fungus liquid of fluid infusion intracavity, and the fungus liquid of stock solution intracavity can not flow through the fungus liquid delivery outlet. And after the bacteria liquid meets the requirements, pressing the inoculating needle disk towards the limiting block again to perform secondary puncture, so that the puncture of one plant body is completed.
Claims (10)
1. A needle-punching type inoculation method for plant germs is characterized in that a plurality of inoculation needles are fixed on an inoculation needle disc with a hand grip structure, the inoculation needles are attached with bacteria liquid, the hand grip structure is held to move all the inoculation needles to pierce leaves or stems of plants to form puncture holes, and the bacteria liquid is transferred into the puncture holes when the inoculation needles form the puncture holes to complete inoculation.
2. The utility model provides a novel acupuncture formula plant germ inoculation equipment, includes the inoculating needle, its characterized in that still includes the inoculating needle dish, one side of inoculating needle dish is equipped with tongs structure, opposite side rigid coupling has a plurality ofly the inoculating needle, the inoculating needle dish is worn to establish in the inoculating needle protection casing, inoculating needle dish and inoculating needle protection casing enclose into inoculating needle protection chamber, the inoculating needle is located in the inoculating needle protection intracavity, be equipped with the stopper on the inner peripheral surface of inoculating needle protection casing, the inoculating needle dish supports when on the stopper the inoculating needle with be equipped with the clearance between the inoculating needle protection casing.
3. The novel needlepunch plant pathogen inoculation device as recited in claim 2, wherein the gripper structure comprises a holding block and a connecting handle having one end connected to the holding block, the other end of the connecting handle being connected to a side of the inoculation tray away from the inoculation needle.
4. The novel needle-punching type plant pathogen inoculation device according to claim 2, wherein the inoculation needle protection cover comprises a tube body and an end plate, the end plate is provided with a skirt, the end plate is connected to the tube body through the skirt in a threaded manner and is detachably connected with the tube body, the inoculation plate is supported on the limiting block through a compression spring, the needle tip of the inoculation needle is completely accommodated in the tube body, and the needle tip of the inoculation needle extends out of the tube body when the inoculation plate is pressed to the limiting block to the limiting position.
5. The novel needlepunch plant pathogen inoculation device of claim 4, wherein the skirt is threaded onto the inner circumferential surface of the tube body.
6. The novel needle-prick plant pathogen inoculation device according to claim 4, wherein the skirt is provided with a blade access hole for the blade of the inoculated plant to access; when the skirt edge rotates towards the interior of the tube body to the limit position, the blade inlet and outlet holes are completely shielded by the tube body; the inoculating tray is pressed to the limit position towards the direction of the limiting block when the blade inlet and outlet holes are exposed outside the tube body, and a gap is formed between the needle tip of the inoculating needle and the end plate.
7. The novel needle-punching plant pathogen inoculation device according to claim 6, wherein the inner surface of the end plate is provided with an elastic supporting pad for supporting the leaves penetrating into the skirt edge through the leaf inlet and outlet holes, and the elastic supporting pad is provided with an inoculation needle avoiding hole aligned with the inoculation needle.
8. The novel needle-punching plant pathogen inoculation device according to claim 7, wherein the inoculation needles are distributed along the circumferential direction of the end plate, and the inoculation needle avoiding hole is in the shape of a ring extending along the circumferential direction of the end plate.
9. The novel needling type plant pathogen inoculation equipment according to claim 4, wherein the hand in the gripper structure is provided with a slide hole extending along the axial direction of the inoculation needle disc, the slide hole is provided with an avoidance through groove and a limiting groove extending along the extension direction of the slide hole, a first piston and a second piston are arranged in the slide hole, the first piston and the second piston are separated into a liquid storage cavity and a liquid supplementing cavity in the slide hole, the peripheral surface of the inoculation needle is provided with a bacteria liquid output port, the liquid storage cavity is communicated with the bacteria liquid conveying port through a bacteria liquid conveying channel, the first piston is connected with the inoculation needle protective cover through a fixed rod penetrating through the avoidance through groove, bacteria liquid and air are stored in the first bacteria liquid storage cavity, bacteria liquid is stored in the liquid supplementing cavity, the second piston is connected with a push rod extending out of the slide hole, the first piston is provided with a limiting bulge positioned in the limiting groove and a piston part channel communicated with the end surface of the limiting bulge and the liquid storage cavity, a check valve which is opened towards the liquid storage cavity is arranged in the piston part channel, a transfer cavity is arranged in the gripper structure, the transfer cavity is provided with a first communicating hole and a second communicating hole which are communicated with the sliding hole, a plug for sealing the first communicating hole and a plug ejecting spring for driving the plug to extend out of the first communicating hole and protrude out of the limiting groove, and a plug part channel for communicating the end face of the plug and the transfer cavity is arranged in the plug; when the inoculating needle disc moves to the position where the fixed rod is abutted with the end wall of the avoiding through groove in the direction away from the limiting block, the plug is abutted with the limiting protrusion, the piston part channel is communicated with the plug part channel, and the second communication hole is communicated with the transfer cavity and the liquid supplementing cavity.
10. The novel needling type plant pathogen inoculation device according to claim 9, wherein the peripheral surface of the second piston is provided with insertion holes, the wall surface of the sliding hole is provided with a pin hole, a pin for inserting into the insertion hole to fix the second piston and the sliding hole together and a pin ejection spring for driving the pin to extend out of the pin hole are arranged in the pin hole, and the pin is provided with a guide surface for guiding the pin to be inserted into the insertion hole and pulled out of the insertion hole; when the plug and the limiting protrusion are aligned, the bolt hole is located in one side, far away from the first piston, of the second piston.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010115210.0A CN111345169B (en) | 2020-02-25 | 2020-02-25 | Needle-punching type plant germ inoculation method and needle-punching type plant germ inoculation equipment |
Applications Claiming Priority (1)
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| CN111345169B (en) | 2021-09-07 |
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