CN202849293U - Device for purifying and decoloring antimicrobial peptide - Google Patents
Device for purifying and decoloring antimicrobial peptide Download PDFInfo
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- CN202849293U CN202849293U CN 201220472039 CN201220472039U CN202849293U CN 202849293 U CN202849293 U CN 202849293U CN 201220472039 CN201220472039 CN 201220472039 CN 201220472039 U CN201220472039 U CN 201220472039U CN 202849293 U CN202849293 U CN 202849293U
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- chromatography column
- liquid
- ultrafiltration
- antibacterial peptide
- decolouring
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Abstract
The utility model discloses a device for purifying and decoloring antimicrobial peptide. The device comprises a centrifugal device, an ultrafiltration device, a chromatographic device and a decoloring device. The centrifugal device comprises a centrifugal machine, a liquid dropping tube, a solid dropping tube and an observation window. The ultrafiltration device comprises multiple levels of ultrafiltration chambers and an ultrafiltration pump. Water outlet tubes and water inlet tubes passing through the ultrafiltration chambers are connected with one another level by level. The chromatographic device comprises a chromatographic column, a liquid adding tube and a liquid outlet. The chromatographic column is internally filled with large-aperture resin, the liquid adding tube is connected with a liquid outlet of the ultrafiltration device, and the chromatographic device is connected with a pressure pump. The decoloring device comprises a decoloring column, a liquid adding tube and a liquid outlet. The liquid adding tube of the decoloring column is connected with a liquid outlet of the chromatographic column. According to the device for purifying and decoloring the antimicrobial peptide through centrifugal, ultra-filtering, chromatographic and decoloring technologies, the purity of the antimicrobial peptide reaches above 90%, and a pure antimicrobial peptide product which meets the criteria of Chinese pharmacopoeia is obtained. The device has the advantages of high production efficiency, good purifying effect, low possibility in pollution and the like, and is suitable for large-scale industrialized continuous production.
Description
Technical field
The utility model relates to a kind of purification devices of bioengineering field, relates in particular to a kind of device for the decolouring of antibacterial peptide purifying.
Background technology
Along with global economic integration, food safety has become the important public health problem of a global challenge and the whole world, is subject to international coverage and pays much attention to, and the safety of animal-derived food is the focus of whole world concern especially.Feeding antibiotic has caused governments at all levels and consumers in general's very big concern to the adverse consequences that human health and living environment cause, and requires the cry of " nonreactive cultured product " also more and more higher on the market.Yet, when not adding microbiotic in the feed, animal morbidity, mortality ratio height, the cultivated animals production performance can degradation.In order to ensure the quality safety of China's animal-derived food, Development and Production safely and efficiently novel feeding antibiotic substitute products is extremely urgent, the substitute antibiotics product of " safety, green, the environmental protection " research topic that become international.
The research and development of antibacterial peptide have demonstrated fully the developing direction of fodder additives industry " safety, efficient, noresidue, pollution-free ".Antibacterial peptide itself is a kind of small protein matter (peptide), is the natural product by the naturally occurring microorganisms of nature, and without any side effects, noresidue has been considered to green additive.On the one hand, it has broad-spectrum antimicrobial and certain antivirus action, can control, prevent Animal diseases, improves animal health, reduces the effect of the excessive use of microbiotic; On the other hand, it is the immunizing power of strong animal body once, improves the micro ecology of gastrointestinal tract environment, improves efficiency of feed utilization.Thereby in feed, use the animal product that antibacterial peptide can obtain high-quality, safety.Novel fodder additive take antibacterial peptide as representative, both substitute antibiotics series products effectively can promote again the growth of livestock and poultry with healthy, showed good development features.
The production technique of antibacterial peptide generally comprises directed screening and obtains antibacterial peptide high yield mutagenesis bacterium, carry out microbial fermentation and obtain containing the fermented liquid of high-density antibacterial peptide, fermented liquid is carried out the multistep purifying, because of its complex process, purification effect is undesirable, thus the yielding poorly of antibacterial peptide, cost high, be difficult to a large amount of production and become the bottlenecks that antibacterial peptide is produced.
The utility model content
The purpose of this utility model provides a kind of device for the decolouring of antibacterial peptide purifying, this device is by being grouped together centrifugal device, ultra-filtration equipment, chromatographic apparatus and decoloration device by certain technical scheme, form integrated antibacterial peptide purifying decolouring treatment unit, it is highly purified not only antibacterial peptide to be obtained by the utility model, and can realize that the purifying decolouring heavy industrialization of antibacterial peptide carries out continuously.
Technical scheme provided by the utility model is:
A kind of device for the decolouring of antibacterial peptide purifying comprises:
Centrifugal device, described centrifugal device comprise whizzer, declining liquid tube and the solid material pipe that falls, and described declining liquid tube is arranged at described whizzer top, describedly fall to admittedly expecting that pipe is arranged at described whizzer bottom;
Ultra-filtration equipment, described ultra-filtration equipment comprises the multistage ultrafiltration chamber that is communicated with successively, and be one-level ultrafiltration chamber along first ultrafiltration chamber in the described multi-ultrafiltration of the direction of the liquid-flow chamber, last ultrafiltration chamber is final stage ultrafiltration chamber, wherein, the liquid inlet of the one-level ultrafiltration chamber in the described multi-ultrafiltration chamber is communicated with the declining liquid tube of described centrifugal device;
Chromatographic apparatus, described chromatographic apparatus comprises chromatography column, chromatography column liquid adding bottle and chromatography column liquid outlet, described chromatography column liquid adding bottle is installed on the top of described chromatography column, described chromatography column liquid outlet is arranged at the mid-way of described chromatography column bottom, described chromatography column liquid adding bottle be connected the liquid outlet of the final stage ultrafiltration chamber in the ultra-filtration equipment and connect;
Decoloration device, described decoloration device comprises decolorizing column, decolorizing column liquid adding bottle and decolorizing column liquid outlet, described decolorizing column liquid adding bottle is installed on the top of described decolorizing column, described decolorizing column liquid outlet is arranged at the mid-way of described decolorizing column bottom, described decolorizing column liquid adding bottle be connected the chromatography column liquid outlet and connect.
Preferably, described device for the decolouring of antibacterial peptide purifying, declining liquid tube quantity in the described centrifugal device is a plurality of, described a plurality of declining liquid tube is distributed in the side on described whizzer top along the short transverse of described whizzer, and jointly be connected to a common conduit, described common conduit is communicated with the liquid inlet of described one-level ultrafiltration chamber, is provided with a valve on described each declining liquid tube.
Preferably, described device for the decolouring of antibacterial peptide purifying, the side of the whizzer in the described centrifugal device is provided with viewing window.
Preferably, described device for antibacterial peptide purifying decolouring, the final stage ultrafiltration chamber in the described ultra-filtration equipment with is connected ultrafiltrate pump and connects.
Preferably, described device for antibacterial peptide purifying decolouring, the indoor ultra-filtration membrane that all is equipped with of each ultrafiltration in the described ultra-filtration equipment, and reduce step by step along the aperture of the ultra-filtration membrane of the described multi-ultrafiltration of the direction of liquid-flow chamber.
Preferably, described device for the decolouring of antibacterial peptide purifying, chromatography column liquid adding bottle in the described chromatographic apparatus and the decolorizing column liquid adding bottle in the described decoloration device are annular, and are provided with a plurality of spray holes below described chromatography column liquid adding bottle and described decolorizing column liquid adding bottle.
Preferably, described device for the decolouring of antibacterial peptide purifying, chromatography column bottom hemisphere in the described chromatographic apparatus, be evenly distributed with a plurality of the first diversion trenchs on the inwall of the bottom of described chromatography column, and described a plurality of the first diversion trench distributes as the center of circle radially take described chromatography column liquid outlet, and described the first diversion trench is bar-shaped trough; The bottom of described decolorizing column is hemisphere, and is evenly distributed with a plurality of the second diversion trenchs on its inwall, and described a plurality of the second diversion trenchs distribute as the center of circle radially take described decolorizing column liquid outlet, and described the second diversion trench also is bar-shaped trough.
Preferably, described device for antibacterial peptide purifying decolouring, described chromatography column with is connected pressure pump and connects.
Preferably, described device for the decolouring of antibacterial peptide purifying is dosed the wide aperture resin in the chromatography column in the described chromatographic apparatus.
Preferably, described device for the decolouring of antibacterial peptide purifying is dosed thing and is in turn from top to bottom Zeo-karb, anionite-exchange resin and quartz sand filter media in the decolorizing column in the described decoloration device.
Described device for antibacterial peptide purifying decolouring is by integrating centrifugal, ultrafiltration, chromatography and decoloration device by certain technical scheme, the purifying decolouring of antibacterial peptide is realized easily, the purity of the antibacterial peptide of processing through this device reaches more than 90%, be fit to the decolouring of heavy industrialization continuous purification and process, have the advantages such as production efficiency height, good, the difficult pollution of purification effect.
Description of drawings
Fig. 1 is the structural representation of the device for antibacterial peptide purifying decolouring described in the utility model.
Embodiment
Below in conjunction with accompanying drawing the utility model is elaborated, after making those of ordinary skills consult this specification sheets, can implement according to this.
As shown in Figure 1, device for the decolouring of antibacterial peptide purifying described in the utility model comprises: centrifugal device, described centrifugal device comprise whizzer 1, declining liquid tube 2 and fall solid material pipe 3, described declining liquid tube is arranged at described whizzer top, describedly falls to admittedly expecting that pipe is arranged at described whizzer bottom; Ultra-filtration equipment, described ultra-filtration equipment comprises the multistage ultrafiltration chamber 5 that is communicated with successively, and be one-level ultrafiltration chamber along first ultrafiltration chamber in the described multi-ultrafiltration of the direction of the liquid-flow chamber 5, last ultrafiltration chamber is final stage ultrafiltration chamber, wherein, the liquid inlet of the one-level ultrafiltration chamber in the described multi-ultrafiltration chamber 5 is communicated with the declining liquid tube 2 of described centrifugal device; Chromatographic apparatus, described chromatographic apparatus comprises chromatography column 6, chromatography column liquid adding bottle 7 and chromatography column liquid outlet 8, described chromatography column liquid adding bottle 7 is installed on the top of described chromatography column 6, described chromatography column liquid outlet 8 is arranged at the mid-way of described chromatography column 6 bottoms, described chromatography column liquid adding bottle 7 be connected the liquid outlet of the final stage ultrafiltration chamber in the ultra-filtration equipment and connect.Decoloration device, described decoloration device comprises decolorizing column 9, decolorizing column liquid adding bottle 10 and decolorizing column liquid outlet 11, described decolorizing column liquid adding bottle 10 is installed on the top of described decolorizing column 9, described decolorizing column liquid outlet 11 is arranged at the mid-way of described decolorizing column 9 bottoms, described decolorizing column liquid adding bottle 10 be connected chromatography column liquid outlet 8 and connect.
Described device for the decolouring of antibacterial peptide purifying, declining liquid tube 2 quantity in the described centrifugal device are a plurality of, described a plurality of declining liquid tube 2 is distributed in the side on described whizzer 1 top along the short transverse of described whizzer 1, and jointly be connected to a common conduit, described common conduit is communicated with the liquid inlet of described one-level ultrafiltration chamber, be provided with a valve on described each declining liquid tube 2, there is transparent viewing window 4 whizzer 1 side in the described centrifugal device, after centrifugal finishing, check the level attitude of solid-liquid separation face by viewing window 4, open the valve of the corresponding declining liquid tube 2 of this level attitude, close simultaneously other declining liquid tube 2 valves, supernatant liquid is flowed out from the declining liquid tube 2 of opening.
Described device for antibacterial peptide purifying decolouring, the final stage ultrafiltration chamber in the described ultra-filtration equipment with is connected ultrafiltrate pump 12 and connects, make the certain suction filtration pressure of generations in the ultrafiltration chamber 5, the efficient of increase ultrafiltration.
Described device for the decolouring of antibacterial peptide purifying, described device for the decolouring of antibacterial peptide purifying, each ultrafiltration chamber 5 interior ultra-filtration membrane that all are equipped with in the described ultra-filtration equipment, and the aperture along the ultra-filtration membrane of the described multi-ultrafiltration of the direction of liquid-flow chamber 5 reduces step by step, hold back step by step the impurity in the solution, finish preliminary filtration.
Described device for the decolouring of antibacterial peptide purifying, chromatography column liquid adding bottle 7 in the described chromatographic apparatus and the decolorizing column liquid adding bottle 10 in the described decoloration device are annular, and be provided with a plurality of spray holes below described chromatography column liquid adding bottle 7 and described decolorizing column liquid adding bottle 10, the solution that contains antibacterial peptide that pressure pump 13 is pumped into evenly is sprayed onto dosing on the thing in chromatography column 6 and the decolorizing column 9.
Described device for the decolouring of antibacterial peptide purifying, chromatography column 6 bottoms in the described chromatographic apparatus are hemisphere, be evenly distributed with a plurality of the first diversion trenchs on the inwall of the bottom of described chromatography column 6, and described a plurality of the first diversion trench distributes as the center of circle radially take described chromatography column liquid outlet 8, and described the first diversion trench is bar-shaped trough; The bottom of described decolorizing column 9 is hemisphere, and be evenly distributed with a plurality of the second diversion trenchs on its inwall, described a plurality of the second diversion trench distributes as the center of circle radially take described decolorizing column liquid outlet 11, and described the second diversion trench is bar-shaped trough also, makes the solution that oozes out flow into liquid outlet through diversion trench.
Described device for antibacterial peptide purifying decolouring, described chromatography column 6 with is connected pressure pump 13 and connects, the liquid that contains antibacterial peptide is pumped in chromatography column 6 and the dehydration column 9.
Described device for the decolouring of antibacterial peptide purifying, dosing thing in the chromatography column 6 in the described chromatographic apparatus is the wide aperture resin.
Described device for the decolouring of antibacterial peptide purifying is dosed thing and is in turn from top to bottom Zeo-karb, anionite-exchange resin and quartz sand filter media in the decolorizing column 9 in the described decoloration device.
Although embodiment of the present utility model is open as above, but it is not restricted to listed utilization in specification sheets and the embodiment, it can be applied to the various fields of the present utility model that are fit to fully, for those skilled in the art, can easily realize other modification, therefore do not deviating under the universal that claim and equivalency range limit, the utility model is not limited to specific details and illustrates here and the legend of describing.
Claims (10)
1. a device that is used for the decolouring of antibacterial peptide purifying is characterized in that, comprising:
Centrifugal device, described centrifugal device comprise whizzer, declining liquid tube and the solid material pipe that falls, and described declining liquid tube is arranged at described whizzer top, describedly fall to admittedly expecting that pipe is arranged at described whizzer bottom;
Ultra-filtration equipment, described ultra-filtration equipment comprises the multistage ultrafiltration chamber that is communicated with successively, and be one-level ultrafiltration chamber along first ultrafiltration chamber in the described multi-ultrafiltration of the direction of the liquid-flow chamber, last ultrafiltration chamber is final stage ultrafiltration chamber, wherein, the liquid inlet of the one-level ultrafiltration chamber in the described multi-ultrafiltration chamber is communicated with the declining liquid tube of described centrifugal device;
Chromatographic apparatus, described chromatographic apparatus comprises chromatography column, chromatography column liquid adding bottle and chromatography column liquid outlet, described chromatography column liquid adding bottle is installed on the top of described chromatography column, described chromatography column liquid outlet is arranged at the mid-way of described chromatography column bottom, described chromatography column liquid adding bottle be connected the liquid outlet of the final stage ultrafiltration chamber in the ultra-filtration equipment and connect;
Decoloration device, described decoloration device comprises decolorizing column, decolorizing column liquid adding bottle and decolorizing column liquid outlet, described decolorizing column liquid adding bottle is installed on the top of described decolorizing column, described decolorizing column liquid outlet is arranged at the mid-way of described decolorizing column bottom, described decolorizing column liquid adding bottle be connected the chromatography column liquid outlet and connect.
2. the device for antibacterial peptide purifying decolouring as claimed in claim 1, it is characterized in that, declining liquid tube quantity in the described centrifugal device is a plurality of, described a plurality of declining liquid tube is distributed in the side on described whizzer top along the short transverse of described whizzer, and jointly be connected to a common conduit, described common conduit is communicated with the liquid inlet of described one-level ultrafiltration chamber, is provided with a valve on described each declining liquid tube.
3. the device for the decolouring of antibacterial peptide purifying as claimed in claim 1 or 2 is characterized in that the side of the whizzer in the described centrifugal device is provided with viewing window.
4. the device for antibacterial peptide purifying decolouring as claimed in claim 1 is characterized in that, the final stage ultrafiltration chamber in the described ultra-filtration equipment with is connected ultrafiltrate pump and connects.
5. such as claim 1 or 4 described devices for antibacterial peptide purifying decolouring, it is characterized in that, the indoor ultra-filtration membrane that all is equipped with of each ultrafiltration in the described ultra-filtration equipment, and reduce step by step along the aperture of the ultra-filtration membrane of the described multi-ultrafiltration of the direction of liquid-flow chamber.
6. the device for antibacterial peptide purifying decolouring as claimed in claim 1, it is characterized in that, chromatography column liquid adding bottle in the described chromatographic apparatus and the decolorizing column liquid adding bottle in the described decoloration device are annular, and are provided with a plurality of spray holes below described chromatography column liquid adding bottle and described decolorizing column liquid adding bottle.
7. the device for antibacterial peptide purifying decolouring as claimed in claim 1, it is characterized in that, chromatography column bottom hemisphere in the described chromatographic apparatus, be evenly distributed with a plurality of the first diversion trenchs on the inwall of the bottom of described chromatography column, and described a plurality of the first diversion trench distributes as the center of circle radially take described chromatography column liquid outlet, and described the first diversion trench is bar-shaped trough; The bottom of described decolorizing column is hemisphere, and is evenly distributed with a plurality of the second diversion trenchs on its inwall, and described a plurality of the second diversion trenchs distribute as the center of circle radially take described decolorizing column liquid outlet, and described the second diversion trench also is bar-shaped trough.
8. such as claim 1,6 or 7 described devices for antibacterial peptide purifying decolouring, it is characterized in that, described chromatography column with is connected pressure pump and connects.
9. such as claim 1,6 or 7 described devices for the decolouring of antibacterial peptide purifying, it is characterized in that, dose the wide aperture resin in the chromatography column in the described chromatographic apparatus.
10. such as claim 1,6 or 7 described devices for the decolouring of antibacterial peptide purifying, it is characterized in that, dose thing in the decolorizing column in the described decoloration device and be in turn from top to bottom Zeo-karb, anionite-exchange resin and quartz sand filter media.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220472039 CN202849293U (en) | 2012-09-14 | 2012-09-14 | Device for purifying and decoloring antimicrobial peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220472039 CN202849293U (en) | 2012-09-14 | 2012-09-14 | Device for purifying and decoloring antimicrobial peptide |
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| Publication Number | Publication Date |
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| CN202849293U true CN202849293U (en) | 2013-04-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220472039 Expired - Fee Related CN202849293U (en) | 2012-09-14 | 2012-09-14 | Device for purifying and decoloring antimicrobial peptide |
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| Country | Link |
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| CN (1) | CN202849293U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106632587A (en) * | 2016-12-29 | 2017-05-10 | 艾普博科技（天津）有限公司 | Ultrafiltration device used for separation and purification of corn antioxidant peptide |
| CN108379877A (en) * | 2018-01-30 | 2018-08-10 | 日照金禾博源生化有限公司 | A kind of citric acid solution decolorizing column and its application |
-
2012
- 2012-09-14 CN CN 201220472039 patent/CN202849293U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106632587A (en) * | 2016-12-29 | 2017-05-10 | 艾普博科技（天津）有限公司 | Ultrafiltration device used for separation and purification of corn antioxidant peptide |
| CN108379877A (en) * | 2018-01-30 | 2018-08-10 | 日照金禾博源生化有限公司 | A kind of citric acid solution decolorizing column and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130403 Termination date: 20140914 |
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| EXPY | Termination of patent right or utility model |