CN106632587A - Ultrafiltration device used for separation and purification of corn antioxidant peptide - Google Patents
Ultrafiltration device used for separation and purification of corn antioxidant peptide Download PDFInfo
- Publication number
- CN106632587A CN106632587A CN201611240709.4A CN201611240709A CN106632587A CN 106632587 A CN106632587 A CN 106632587A CN 201611240709 A CN201611240709 A CN 201611240709A CN 106632587 A CN106632587 A CN 106632587A
- Authority
- CN
- China
- Prior art keywords
- layer
- ultrafiltration
- shell
- antioxidant peptide
- isolated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000108 ultra-filtration Methods 0.000 title claims abstract description 59
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 27
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 27
- 235000005822 corn Nutrition 0.000 title claims abstract description 27
- 101800000068 Antioxidant peptide Proteins 0.000 title claims abstract description 20
- 240000008042 Zea mays Species 0.000 title claims description 25
- 238000000746 purification Methods 0.000 title abstract 3
- 238000000926 separation method Methods 0.000 title abstract 3
- 238000001914 filtration Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 7
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 7
- 239000003610 charcoal Substances 0.000 claims abstract description 7
- 229940070527 tourmaline Drugs 0.000 claims abstract description 7
- 229910052613 tourmaline Inorganic materials 0.000 claims abstract description 7
- 239000011032 tourmaline Substances 0.000 claims abstract description 7
- 230000000694 effects Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 2
- 241000209149 Zea Species 0.000 abstract 2
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- 239000000383 hazardous chemical Substances 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 229920002494 Zein Polymers 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000005019 zein Substances 0.000 description 3
- 229940093612 zein Drugs 0.000 description 3
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920002261 Corn starch Polymers 0.000 description 1
- 208000035859 Drug effect increased Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000006035 Tryptophane Substances 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000013777 protein digestion Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Abstract
The invention provides an ultrafiltration device used for separation and purification of corn antioxidant peptide, and belongs to the technical field of ultrafiltration apparatuses. The ultrafiltration device comprises a shell, the shell is internally provided with an ultrafiltration layer, the ultrafiltration layer is overlaid by multiple ultrafiltration units, a gap is arranged between the ultrafiltration layer and the shell, the center of the ultrafiltration layer is provided with a cavity, the ultrafiltration layer is driven by a motor, the motor is located at the bottom end of the shell, the top part of the cavity is provided with a primary filtration tube, the primary filtration tube is internally provided with a filter screen layer, a cocoanut active charcoal layer and a tourmaline layer from top to bottom in sequence, the top part of the primary filtration tube is provided with a liquid inlet, and the bottom part of the shell is provided with a liquid outlet. The ultrafiltration device used for separation and purification of the corn antioxidant peptide is reasonable in design, simple in structure, good in filtration effect and high in filtration efficiency; existence of hazardous substances and germs in the separated product is avoided, the time and labor are saved, the human cost is saved, and the enterprise benefit is increased.
Description
Technical field
The present invention relates to mixing plant technical field, specifically a kind of corn antioxidant peptide is isolated and purified uses ultrafiltration apparatus.
Background technology
Corn protein powder is the principal by product in production of corn starch, predominantly beautiful containing about 60% protein
Rice alcohol soluble protein (68%), lonely white (22%) and globulin (1.2%).Because corn protein powder constitutes complicated, poorly water-soluble,
Lysine and tryptophane are very low, limit its application in the food industry, and cheap due to its, now in state
The interior main protein resource that corn protein powder is used for into feed industry, preciousness is so wasted.Not only can by enzymatic hydrolysis
The water solubility of zein is improved, is easy to using the limited protein resource of China, and by further isolating and purifying hydrolysis
Product is obtained with anti-oxidant, hypotensive, the antifatigue, functional characteristic such as sober up.
Corn functional peptides come from the hydrolysate of zein, be due to molecular weight very little (be generally concentrated at 1000 with
Under), but the mixing oligopeptide of the very high small peptide composition of activity.Small-molecular peptides than equivalent amino acid have higher biologically active and
Nutritive value, peptides and amino acid almost can be completely absorbed and used by Epithelium of intestinal villus cell, enter into blood
In, each position of organism is then delivered to, for this purpose, the exploitation of corn functional peptides is with a wide range of applications, Er Qiesui
The development of science and technology, the raising of people's consciousness and the enhancing of health care consciousness, the integration of drinking and medicinal herbs of corn functional peptides will more show
Go out its wide application prospect.But due to from various protein digestion products extract anti-oxidation peptide amino acid composition and
Structural difference is very big, the amino acid composition that neither one is fixed, and enzymolysis product composition is also extremely complex, therefore isolates and purifies
Highly active corn peptide is extremely difficult.Ultrafiltration apparatus are used currently without special isolating and purifying for corn antioxidant peptide, is adopted
Common ultrafiltration apparatus can not meet the needs for isolating and purifying.
The content of the invention
In view of this, the present invention is intended to provide a kind of reasonable in design, simple structure, good filtration effect, filter efficiency is high,
The presence of harmful substance and bacterium is reduced in the product separated, it is time saving and energy saving, human cost is saved, improve the performance of enterprises
Corn antioxidant peptide is isolated and purified uses ultrafiltration apparatus.
To reach above-mentioned purpose, the technical scheme is that what is be achieved in that：A kind of corn antioxidant peptide is isolated and purified
With ultrafiltration apparatus, including a shell, the inside the shell is provided with ultrafiltration layer, there is gap between the ultrafiltration layer and shell, described super
The center of filtering layer is provided with a cavity, and the ultrafiltration layer is located at the bottom of housing by a Motor drive, the motor, the cavity
Top is provided with a first chimney filter, and in the just chimney filter the first filtering layer, the second filtering layer and the 3rd filtering layer are sequentially provided with from top to bottom, described
Just a liquid inlet is provided with the top of chimney filter, the bottom of the shell is provided with a liquid outlet.
Further, first filtering layer is filter mesh layer.
Further, second filtering layer is coconut activated layer of charcoal.
Further, the 3rd filtering layer is Tourmaline layer.
Further, the liquid inlet is horn-like structure.
Further, the bottom of the ultrafiltration layer is provided with a base plate, and the output end of the motor is fixed on base plate.
Further, the ultrafiltration layer is formed by stacking by several ultra filtration units.
Further, the ultra filtration unit is four.
Further, the cross-sectional area of the ultra filtration unit is hexagonal structure.
Relative to prior art, the present invention has the advantage that：
Corn antioxidant peptide of the present invention is isolated and purified uses ultrafiltration apparatus, reasonable in design, simple structure, good filtration effect, mistake
Filter efficiency high, reduces the presence of harmful substance and bacterium in the product separated, time saving and energy saving, saves human cost, improves
The performance of enterprises.
Description of the drawings
The accompanying drawing for constituting the part of the present invention is used for providing a further understanding of the present invention, the schematic reality of the present invention
Apply example and its illustrate, for explaining the present invention, not constituting inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 is the structural representation of the present invention.
In figure：
1st, shell；2nd, ultrafiltration layer；3rd, ultra filtration unit；4th, gap；5th, cavity；6th, motor；7th, first chimney filter；8th, filter mesh layer；9、
Coconut activated layer of charcoal；10th, Tourmaline layer；11st, liquid inlet；12nd, liquid outlet；13rd, base plate.
Specific embodiment
It should be noted that in the case where not conflicting, the embodiment and the feature in embodiment in the present invention can phase
Mutually combination.
In describing the invention, it is to be understood that term " " center ", " longitudinal direction ", " horizontal ", " on ", D score,
The orientation or position relationship of the instruction such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outward " is
Based on orientation shown in the drawings or position relationship, it is for only for ease of the description present invention and simplifies description, rather than indicates or dark
Showing the device or element of indication must have specific orientation, with specific azimuth configuration and operation therefore it is not intended that right
The restriction of the present invention.Additionally, term " first ", " second " etc. are only used for describing purpose, and it is not intended that indicating or implying phase
To importance or the implicit quantity for indicating indicated technical characteristic.Thus, the feature for defining " first ", " second " etc. can
To express or implicitly include one or more this feature.In describing the invention, unless otherwise stated, " multiple "
It is meant that two or more.
In describing the invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Company ", " connection " should be interpreted broadly, for example, it may be being fixedly connected, or being detachably connected, or be integrally connected；Can
Being to be mechanically connected, or electrically connect；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, Ke Yishi
The connection of two element internals.For the ordinary skill in the art, above-mentioned term can be understood by concrete condition
Concrete meaning in the present invention.
Below with reference to the accompanying drawings and in conjunction with the embodiments describing the present invention in detail.
As shown in figure 1, a kind of corn antioxidant peptide is isolated and purified uses ultrafiltration apparatus, including a shell 1, it is provided with shell 1
Ultrafiltration layer 2, ultrafiltration layer 2 is formed by stacking by four ultra filtration units 3, and the cross-sectional area of ultra filtration unit 3 is hexagonal structure, ultrafiltration
The center for having gap 4, ultrafiltration layer 2 between layer 2 and shell 1 is provided with a cavity 5, and the bottom of ultrafiltration layer 2 is provided with a base plate 13, motor
6 output end is fixed on base plate 13, and motor 6 is located at the bottom of housing 1, and the top of cavity 5 is provided with a first chimney filter 7, just chimney filter 7
Filter mesh layer 8, coconut activated layer of charcoal 9 and Tourmaline layer 10 are inside sequentially provided with from top to bottom, and just the top of chimney filter 7 is provided with a liquid and enters
Mouth 11, liquid inlet 11 is horn-like structure, and the bottom of shell 1 is provided with a liquid outlet 12.
The implementation process of the present embodiment：When using, by detached zein to be purified, hydrolase and solvent etc. from liquid
Body entrance 11 is slowly added to just consider in pipe 7, and mixed liquor passes sequentially through filter mesh layer 8, coconut activated layer of charcoal 9 and Tourmaline layer 10, filter
Stratum reticulare 8 can effectively remove the larger particles such as impurity, the suspension in mixed liquor, and coconut activated layer of charcoal 9 can effectively remove mixed liquor
In suspension, heterochromatic and peculiar smell, Tourmaline layer 10 can effectively remove bacterium etc., and the mixed liquor after filtration is entered in cavity 5, electricity
Machine 6 drives ultrafiltration layer 2 to rotate, and the mixed liquor in cavity 5 enters under the influence of centrifugal force ultrafiltration layer 2, due to the centrifugation of liquid
Effect increased pressure differential of the liquid on ultrafiltration layer 2, accelerate the speed that liquid passes through in ultrafiltration layer 2, the water of small molecule and
Soluble ion is entered in gap 4 by ultrafiltration layer 2, and is discharged by liquid outlet 12, and the corn antioxidant peptide of needs is stayed in
In cavity 5.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (9)
1. a kind of corn antioxidant peptide is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：Including a shell, the inside the shell is provided with
Ultrafiltration layer, the center for having gap, the ultrafiltration layer between the ultrafiltration layer and shell is provided with a cavity, and the ultrafiltration layer is electric by one
Machine drives, and the motor is located at the bottom of housing, a first chimney filter is provided with the top of the cavity, in the just chimney filter from top to bottom
The first filtering layer, the second filtering layer and the 3rd filtering layer are sequentially provided with, at the top of the just chimney filter liquid inlet is provided with, the shell
Bottom is provided with a liquid outlet.
2. corn antioxidant peptide according to claim 1 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：First filter
Layer is filter mesh layer.
3. corn antioxidant peptide according to claim 1 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：Second filter
Layer is coconut activated layer of charcoal.
4. corn antioxidant peptide according to claim 1 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：3rd filter
Layer is Tourmaline layer.
5. corn antioxidant peptide according to claim 1 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：The liquid enters
Mouth is horn-like structure.
6. corn antioxidant peptide according to claim 1 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：The ultrafiltration layer
Bottom be provided with a base plate, the output end of the motor is fixed on base plate.
7. corn antioxidant peptide according to claim 1 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：The ultrafiltration layer
Be formed by stacking by several ultra filtration units.
8. corn antioxidant peptide according to claim 7 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：The ultrafiltration list
Unit is four.
9. corn antioxidant peptide according to claim 7 is isolated and purified and uses ultrafiltration apparatus, it is characterised in that：The ultrafiltration list
The cross-sectional area of unit is hexagonal structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611240709.4A CN106632587A (en) | 2016-12-29 | 2016-12-29 | Ultrafiltration device used for separation and purification of corn antioxidant peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611240709.4A CN106632587A (en) | 2016-12-29 | 2016-12-29 | Ultrafiltration device used for separation and purification of corn antioxidant peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106632587A true CN106632587A (en) | 2017-05-10 |
Family
ID=58837204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611240709.4A Pending CN106632587A (en) | 2016-12-29 | 2016-12-29 | Ultrafiltration device used for separation and purification of corn antioxidant peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106632587A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000354702A (en) * | 1999-06-10 | 2000-12-26 | Academia Sinica | Device and method for refining sample |
| CN202829730U (en) * | 2012-10-05 | 2013-03-27 | 山东格瑞水务有限公司 | Reverse osmosis water purification device |
| CN202849293U (en) * | 2012-09-14 | 2013-04-03 | 农鑫高科（北京）科技开发有限公司 | Device for purifying and decoloring antimicrobial peptide |
| CN104275092A (en) * | 2014-09-17 | 2015-01-14 | 重庆澳龙生物制品有限公司 | Ultrafiltration system of biological product |
| CN104289107A (en) * | 2014-09-17 | 2015-01-21 | 重庆澳龙生物制品有限公司 | Multi-level filter type ultrafiltration system |
| CN105819600A (en) * | 2016-05-19 | 2016-08-03 | 苏州优斯曼环保科技有限公司 | Ultrafilter |
| CN205740833U (en) * | 2016-06-30 | 2016-11-30 | 扬州工业职业技术学院 | A kind of gamma aminobutyric acid fermentation liquid purification devices |
-
2016
- 2016-12-29 CN CN201611240709.4A patent/CN106632587A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000354702A (en) * | 1999-06-10 | 2000-12-26 | Academia Sinica | Device and method for refining sample |
| CN202849293U (en) * | 2012-09-14 | 2013-04-03 | 农鑫高科（北京）科技开发有限公司 | Device for purifying and decoloring antimicrobial peptide |
| CN202829730U (en) * | 2012-10-05 | 2013-03-27 | 山东格瑞水务有限公司 | Reverse osmosis water purification device |
| CN104275092A (en) * | 2014-09-17 | 2015-01-14 | 重庆澳龙生物制品有限公司 | Ultrafiltration system of biological product |
| CN104289107A (en) * | 2014-09-17 | 2015-01-21 | 重庆澳龙生物制品有限公司 | Multi-level filter type ultrafiltration system |
| CN105819600A (en) * | 2016-05-19 | 2016-08-03 | 苏州优斯曼环保科技有限公司 | Ultrafilter |
| CN205740833U (en) * | 2016-06-30 | 2016-11-30 | 扬州工业职业技术学院 | A kind of gamma aminobutyric acid fermentation liquid purification devices |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170510 |
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| RJ01 | Rejection of invention patent application after publication |