CN202794187U - Test paper card for rapid detection of salbutamol residue - Google Patents
Test paper card for rapid detection of salbutamol residue Download PDFInfo
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- CN202794187U CN202794187U CN 201220457056 CN201220457056U CN202794187U CN 202794187 U CN202794187 U CN 202794187U CN 201220457056 CN201220457056 CN 201220457056 CN 201220457056 U CN201220457056 U CN 201220457056U CN 202794187 U CN202794187 U CN 202794187U
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Abstract
The utility model discloses a test paper card for rapid detection of salbutamol residue. The technical scheme adopted by the utility model is as follows: the test paper card for rapid detection of the salbutamol residue comprises a plastic box body and a test strip encapsulated in the plastic box body, wherein the bottom layer of the test strip is a support layer, a sample pad, a gold-labelled antibody bonding pad, a nitrocellulose membrane and an absorption pad which are tightly connected are sequentially adhered to the support layer from left to right, the plastic box body is provided with a sample adding hole and an observation window, position of the sample adding hole is corresponding to that of the sample pad on the test strip, and the position of the observation window is corresponding to that of the nitrocellulose membrane on the test strip. The test paper card for rapid detection of the salbutamol residue disclosed by the utility model is simple to use, rapid and accurate, high in sensitivity, low in cost and good in stability and can be used for realizing batch inspection.
Description
Technical field
The utility model belongs to immunochemistry detection technique field, is specifically related to a kind of salbutamolum residue quick detection test paper card.
Background technology
Quick, intensivization development along with animal husbandry, the non-treatments such as promotion growth significantly increase with the application of veterinary drug in the animal husbandry production run, and the animal derived food medicament residue that causes thus also is on the rise to consumer's health and the potential hazard of environment.From the eighties in last century, the western developed countries such as European Union are just prohibited and are used synthetic hormone and restriction to use veterinary drug in animal feeding, and China also explicitly points out " produce feed and feed addictive and must not add hormone medicine " in new " feed and feed addictive management rules " of promulgating.But in recent years, " clenbuterol hydrochloride " clenobuterol hydrochloride often is found illegally to be added in the animal feed to increase the remuneration of feed, causes serious clenbuterol hydrochloride poisoning to happen occasionally, and therefore the government regulation dynamics is also constantly strengthened.Yet for escaping supervision, illegal person constantly seeks new " clenbuterol hydrochloride " substitute.
Salbutamol (Salbutamol, SAL), have another name called albuterol, Aerolin, Aerolin etc., chemical name 1-(4-hydroxyl-3-hydroxymethyl phenyl)-uncle's 2-(fourth is amino) ethanol, with the special logical sequence in hydrochloric acid Crow, Ractopamine is the same is a kind of phenol amine beta-adrenaline excitant, the effects such as lax bronchial smooth muscle are arranged, prevent and treat the respiratory diseases such as bronchial astehma and asthma type chronic bronchitis, pulmonary emphysema medically being widely used in.Salbutamol has nutrition reallocation effect, causes that by the synthetic of stimulating protein the fiber finer intracellular organic matter increases, and volume increases, and protein degradation slows down Adipogenesis minimizing, and then raising lean meat percentage.Salbutamol is because low production cost, use in livestock products are produced as a kind of novel growth-promoting additive, especially after " clenbuterol hydrochloride " clenobuterol hydrochloride was completely forbidden use, salbutamol just became the common drug that livestock products are produced the herbal medicine adjuvant.But can produce obvious spread effect to the cardiovascular system nervous system of unifying when human body salbutamol intake is excessive, cause that muscle trembles, palpitaition, nervous, headache, dizzy, the symptoms such as that severe patient has is nauseating, vomiting, even threat to life.China Ministry of Agriculture in 2002, the Ministry of Public Health announce 6 kinds of forbidding veterinary drug excitants such as his woods of salbutamol, Cimaterol, special step, Clenbuterol, Ractopamine, dopamine, and require in meat products, must not detect, be that content is less than or equal to 0.1 μ g/kg, therefore, set up science, efficient, easy salbutamolum residue method for supervising is very necessary.
Salbutamolum residue detects main traditional physical and chemical inspection method that relies on, such as high performance liquid chromatography (HPLC), vapor-phase chromatography (GC), gas chromatography-mass spectrometry (GC-MS), LC-MS analysis method (LC-MS) etc., but these methods exist the running program that needs expensive instrument and equipment, very complicated, high expense, can not rig-site utilization etc. defective, be difficult to satisfy the actual monitored demand.The ELISA detection method has high specificity, sample pre-treatments is simple, cost is low, can carry out the advantages such as batch detection, but the salbutamolum residue detection kit of domestic report is less.
The colloid gold label immunoassay is a kind of novel analytical technology that develops rapidly in recent years, be characterized in easy fast, cost is low, pollution-free, need not training, is very suitable for Site Detection.Compare with ELISA, it is simple to have sample pre-treatments, developing time short (3-5 min), and all pack are contained on the test strips, need not to use the advantages such as instrument, have wide market outlook and using value.Therefore, development is quick, sensitive, salbutamolum residue quick detection test paper card is of great significance for guarantee animal food safety tool efficiently.
Summary of the invention
The technical matters that the utility model solves provided a kind of simple to operate, quick and precisely, highly sensitive, cost is low, good stability, can carry out the salbutamolum residue quick detection test paper card of batch detection.
The technical solution of the utility model is: a kind of salbutamolum residue quick detection test paper card, it is characterized in that: described salbutamolum residue quick detection test paper card comprises plastic box body and is packaged in the interior test strips of plastic box body, the bottom of described test strips is supporting layer, on this supporting layer by the left-to-right sample pad that closely links to each other that is attached with successively, gold labeling antibody pad, nitrocellulose filter and absorption pad, have well and view window on the described plastic box body, the position of this well is corresponding with the sample pad position on the test strips, view window is corresponding with the cellulose nitrate film location on the test strips, the detection line (T line) of salbutamol-carrier protein couplet thing solution printing and the nature controlling line (C line) that sheep anti-mouse igg solution is printed are arranged on the described nitrocellulose filter, between the two apart from 5 mm, wherein the detection line (T line) of salbutamol-carrier protein couplet thing solution printing is positioned at the side near sample pad, is perfused with the anti-salbutamol monoclonal antibody of colloid gold label on the described pad.
Supporting layer described in the utility model is to cooperate the PVC plate made from stabilizing agent and auxiliary material by Corvic.Absorption pad described in the utility model is to be the pure white filter paper that starting material are made by the extremely strong plant long fibre of water-intake capacity.Sample pad described in the utility model and golden labeling antibody pad are made by glass fibre cotton.The width that laminates of sample pad described in the utility model and golden labeling antibody pad is 2 mm, and the width that laminates of absorption pad and nitrocellulose filter is 2 mm.Sample pad described in the utility model, golden labeling antibody pad and absorption pad top are coated with rubber protective layer.Nitrocellulose filter described in the utility model two ends boundary has mark line.
The utlity model has following advantage: (1) highly sensitive, high specificity.Salbutamolum residue quick detection test paper card is prepared from the monoclonal antibody of colloid gold label high-affinity, gold grain combines by Van der Waals force between the charges of different polarity with antibody molecule in the gold labeling antibody, and collaurum is very little on specificity and the affinity impact of monoclonal antibody.Therefore, the quick detection test paper jig has higher sensitivity and specificity, and its detection sensitivity can reach 1.0 ng/ml;
(2) simple, convenient quick.Need not other any reagent when using the quick detection test paper card, as long as the sample solution after will processing splashes in the test card well 3 ~ 4 with dropper, 3 ~ 5 min get final product observations;
(3) testing result image, directly perceived.Test card is with red trace line " | " or " ‖ " positive and the negative marker as detection line, when namely nature controlling line (C line) shows a redness " | " trace on nitrocellulose filter, salbutamolum residue content 〉=1.0 ng/ml in the detected sample solution of expression; When if two redness " ‖ " trace appears in nature controlling line on the nitrocellulose filter (C line) and detection line (T line) simultaneously, salbutamolum residue content＜1.0 ng/ml in the expression sample solution.The result is visual in image, and is accurately simple, is not easy to occur erroneous judgement;
(4) use of glued membrane can prolong testing result observing time, test card good stability.This test card absorption of sample pad will detect solution and absorb fully, make it with the coupling pad on golden labeling antibody fully react, can effectively reduce error rate; Can also prevent that introduced contaminants from disturbing, and affects the combination of golden labeling antibody and detectable antigens;
(5) low, the small investment of cost.Use the utility model test card, do not need to join in addition complicated instrument and equipment and expensive reagent, Site Detection settles at one go, and is with low cost, instant effect;
(6) be easy to apply on a large scale.This test card is simple to operate, is fit to different classes of librarian use, such as laboratory detection, customs quarantine control, health supervision, breeding scale and individual production etc., has wide market outlook and larger economic benefit and social benefit.
Description of drawings
Fig. 1 is structural representation of the present utility model; Fig. 2 is the side view of test strips in the utility model; Fig. 3 is the vertical view of test strips in the utility model; Fig. 4 is the negative use state reference map of the utility model testing result; Fig. 5 is the positive use state reference map of the utility model testing result; Fig. 6 is the invalid use state reference map of the utility model testing result.
Embodiment
Describe by reference to the accompanying drawings embodiment in detail.A kind of salbutamolum residue quick detection test paper card, comprise plastic box body 1 and the test strips 2 that is packaged in the plastic box body 1, the bottom of described test strips 2 is supporting layer 3, on this supporting layer 3 by the left-to-right sample pad 4 that closely links to each other that is attached with successively, gold labeling antibody pad 5, nitrocellulose filter 6 and absorption pad 7, have well 8 and view window 9 on the described plastic box body 1, the position of this well 8 is corresponding with sample pad 4 positions on the test strips 2, view window 9 is corresponding with nitrocellulose filter 6 positions on the test strips 2, detection line (the T line) 11 of salbutamol-carrier protein couplet thing solution printing and the nature controlling line (C line) 10 that sheep anti-mouse igg solution is printed are arranged on the described nitrocellulose filter 6, between the two apart from 5 mm, wherein the detection line (T line) 11 of salbutamol-carrier protein couplet thing solution printing is positioned at the side near sample pad 4, is perfused with the anti-salbutamol monoclonal antibody of colloid gold label on the described pad 5.
Supporting layer 3 described in the utility model is to cooperate the PVC plate made from stabilizing agent and auxiliary material by Corvic.Absorption pad 7 described in the utility model is to be the pure white filter paper that starting material are made by the extremely strong plant long fibre of water-intake capacity.Sample pad 4 described in the utility model and golden labeling antibody pad 5 are made by glass fibre cotton.The width that laminates of sample pad 4 described in the utility model and golden labeling antibody pad 5 is 2 mm, and absorption pad 7 is 2 mm with the width that laminates of nitrocellulose filter 6.Sample pad 4 described in the utility model, golden labeling antibody pad 5 and absorption pad 7 tops are coated with rubber protective layer 12.Nitrocellulose filter 6 two ends boundarys described in the utility model have mark line 13.
The preparation method of test card described in the utility model:
1) preparation of nitrocellulose filter: nitrocellulose filter is placed on the unidirectional specking instrument of the X-only platform, detectable antigens is put in the A pond, RaMIgG is put in the B pond, flatten and compress, after the start with antigen and two anti-respectively fixed fires on nitrocellulose filter, form detection line (T line) and nature controlling line (C line).Behind the natural drying at room temperature, it is immersed 30 min in confining liquid (mass concentration is the PBS damping fluid of 1% BSA, and pH 7.4), after 37 ℃ of oven dry, add drying agent, 4 ℃ of sealings are preserved.
2) preparation of pad: glass fibre cotton is cut into the wide slice of 4 mm, puts into that to contain mass concentration be 5% BSA, mass concentration is 2% sucrose, and mass concentration is that 0.8% NaCl and mass concentration are 0.05% NaN
3The PBS treating fluid in 20 min, 37 ℃ of constant temperature dryings, on the glass fibre cotton of then perfusion of golden labeling antibody having been handled well, vacuum freeze-drying 4 h are pad.
3) preparation of sample pad: glass fibre cotton will be 2% BSA with containing mass concentration, and mass concentration is 1% sucrose, and mass concentration is that 0.5% sodium borate and mass concentration are 0.1% NaN
3PBS process after, drying for standby is sample pad.
4) preparation of absorption pad: the plant long fibre that water-intake capacity is extremely strong is that the pure white filter paper that starting material are made is cut into the wide slice of 4 mm with cutting machine, and carry out with glued membrane one side closed, drying for standby.
5) preparation of test strips: on back up pad (PVC plate), nitrocellulose filter, pad, sample pad, absorption pad and glued membrane etc. are fitted together by certain technique, make the wide test strips of 4 mm with the CM4000 cutting machine, the closed container of putting into drying agent stores.
6) assembling of test card: by certain technique test strips is packaged in the special plastic box body with well and view window, is the utility model test card.
The detection principle of test card described in the utility model:
Test card designs according to antigen-antibody competitive immunization chromatographic theory.After dripping sample liquid, under the capillary action of absorption pad and nitrocellulose filter, sample solution is migration upwards, and when arriving pad, golden labeling antibody is with dissolved.When containing salbutamol in the sample, they will with golden labeling antibody combination, and together upwards migration arrives when being fixed with the detection line position of SAL-OVA, detectable antigens will be competed antigen binding site limited on the joining gold labeling antibody with salbutamol.SAL content is higher in the sample, and detectable antigens and golden labeling antibody are just fewer in conjunction with quantity, and the colour developing of T line is just more weak; When SAL in the sample is higher than certain numerical value (1.0 ng/ml), detectable antigens just can't with golden labeling antibody combination, the T line does not develop the color.Whether no matter have SAL to exist in the sample, excessive golden labeling antibody or detectable antigens all will be combined with two anti-RaMIgG with the bond of golden labeling antibody, form redness at the C line.
The using method of test card described in the utility model:
1) sample pre-treatments:
Meat sample: take by weighing 1.0 g samples in 50 ml centrifuge tubes; HCl solution 10 ml that add 0.1 M are with vortex instrument whirling motion 6～8 min; Centrifugal 10 min of 4000 r/min; Get 1 ml supernatant, regulate pH value to 7.0～about 20 ml of 8.0(with 1 M NaOH solution); Centrifugal 5 min of 4000 r/min; Be used for sample analysis after getting the supernatant dilution.
Urine sample: gather the fresh urine sample in the 50 ml left and right sides with dry, clean centrifuge tube or appropriate containers and be put in 4 ℃ of refrigerators to be checkedly, generally do not need special processing, can be directly used in test card and detect; Pollute and muddiness if having in the urine, detect after centrifugal 10 min of 5000 r/min or the filtration.
2) sample drips:
After test card kept flat, with dropper sample solution is splashed in the test card well 3 ~ 4, get final product observations behind 3 ~ 5 min.
3) Analysis of test results:
Negative: as if when two redness " ‖ " trace appears in nature controlling line (C line) and detection line (T line) simultaneously on the nitrocellulose filter, represent salbutamolum residue content＜1.0 ng/ml in the sample solution, to be judged to feminine gender, as shown in Figure 4.
Positive: if nature controlling line (C line) shows a redness " | " trace on the nitrocellulose filter, and detection line (T line) does not develop the color, and salbutamolum residue content 〉=1.0 ng/ml in the detected sample solution of expression are judged to the positive, as shown in Figure 5.
Invalid: if nature controlling line (C line) does not show red stripes on the nitrocellulose filter, then no matter whether detection line (T line) red trace occurs, and it is invalid that this test card all is judged to, as shown in Figure 6.
Claims (7)
1. salbutamolum residue quick detection test paper card, it is characterized in that: described salbutamolum residue quick detection test paper card comprises plastic box body and is packaged in the interior test strips of plastic box body, the bottom of described test strips is supporting layer, on this supporting layer by the left-to-right sample pad that closely links to each other that is attached with successively, gold labeling antibody pad, nitrocellulose filter and absorption pad, have well and view window on the described plastic box body, the position of this well is corresponding with the sample pad position on the test strips, view window is corresponding with the cellulose nitrate film location on the test strips, the detection line of salbutamol-carrier protein couplet thing solution printing and the nature controlling line that sheep anti-mouse igg solution is printed are arranged on the described nitrocellulose filter, between the two apart from 5 mm, wherein the detection line of salbutamol-carrier protein couplet thing solution printing is positioned at the side near sample pad, is perfused with the anti-salbutamol monoclonal antibody of colloid gold label on the described pad.
2. salbutamolum residue quick detection test paper card according to claim 1, it is characterized in that: described supporting layer is the PVC plate.
3. salbutamolum residue quick detection test paper card according to claim 1 is characterized in that: described absorption pad is to be the pure white filter paper that starting material are made by the plant long fibre.
4. salbutamolum residue quick detection test paper card according to claim 1, it is characterized in that: described sample pad and golden labeling antibody pad are made by glass fibre cotton.
5. salbutamolum residue quick detection test paper card according to claim 1, it is characterized in that: the width that laminates of described sample pad and golden labeling antibody pad is 2 mm, the width that laminates of absorption pad and nitrocellulose filter is 2 mm.
6. salbutamolum residue quick detection test paper card according to claim 1 is characterized in that: be coated with rubber protective layer above described sample pad, golden labeling antibody pad and the absorption pad.
7. salbutamolum residue quick detection test paper card according to claim 1, it is characterized in that: described nitrocellulose filter two ends boundary has mark line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220457056 CN202794187U (en) | 2012-09-10 | 2012-09-10 | Test paper card for rapid detection of salbutamol residue |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220457056 CN202794187U (en) | 2012-09-10 | 2012-09-10 | Test paper card for rapid detection of salbutamol residue |
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| CN202794187U true CN202794187U (en) | 2013-03-13 |
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| CN 201220457056 Expired - Fee Related CN202794187U (en) | 2012-09-10 | 2012-09-10 | Test paper card for rapid detection of salbutamol residue |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105911268A (en) * | 2016-05-31 | 2016-08-31 | 中国农业大学 | Colloidal gold test strip detection result automatic reading instrument and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105911268A (en) * | 2016-05-31 | 2016-08-31 | 中国农业大学 | Colloidal gold test strip detection result automatic reading instrument and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130313 Termination date: 20130910 |