CN203385729U - Immunological test paper card for olaquindox - Google Patents
Immunological test paper card for olaquindox Download PDFInfo
- Publication number
- CN203385729U CN203385729U CN201320434942.1U CN201320434942U CN203385729U CN 203385729 U CN203385729 U CN 203385729U CN 201320434942 U CN201320434942 U CN 201320434942U CN 203385729 U CN203385729 U CN 203385729U
- Authority
- CN
- China
- Prior art keywords
- olaquindox
- pad
- test card
- line
- detection test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Abstract
The utility model discloses an immunological test paper card for olaquindox. According to the technical scheme, the test paper card comprises a plastic box body and a test strip packaged in the plastic box body, wherein the bottom layer of the test strip is a supporting layer; a sample pad, a gold-labelled antibody binding pad, a nitrocellulose membrane and an absorbent pad, which are closely connected with each other, are adhered to the supporting layer from left to right in sequence; a sample adding hole and an observation window are formed in the plastic box body; the position of the sample adding hole corresponds to the position of the sample pad on the test strip; the observation window corresponds to the position of the nitrocellulose membrane on the test strip. The test paper card is simple to operate, is quick and accurate, has high sensitivity and good stability, is low in cost and can be used for batch test.
Description
Technical field
The utility model belongs to food security-residue of veterinary drug immunochemistry detection technique field, is specifically related to a kind of immunology detection test card of olaquindox.
Background technology
Olaquindox (olaquindox, OLA) has another name called olaquindox, Olaquindox, chemical name 2-hydroxyethylamino formyl-3-methyl-quinoxaline-1s, and the 4-dioxide is nineteen sixty-five by a kind of quinoxaline veterinary drug microbiotic of German Bayer company exploitation.Its mechanism of action, for by destroying protein synthesis system, folic acid and the RNA synzyme system of pathogenic microorganism, rises and kills and inhibiting effect pathogenic microorganism.Olaquindox can promote protein synthetic, increases nitrogen and stores up, and improves utilization factor and the price of deed of protein in feed, promotes growth of animal.Simultaneously, chemosynthesis antivirus somatotropic agent as widespread use in aquaculture, it also has broad-spectrum antibacterial action, as stronger as effects such as Pasteurella, white diarrhea salmonella, Escherichia coli and proteuss to Gram-negative bacteria, gram-positive bacteria is also had to inhibiting effect as staphylococcus aureus, streptococcus and treponema.
Due to the olaquindox low price, easy to use, so often by overdose, used in actual production or add in violation of rules and regulations, even as the aquatic products clenbuterol hydrochloride, add in fish meal.Research shows: after olaquindox enters animal body, meeting be accumulated residual in RP-HPLC, and mutagenesis and certain genetoxic are arranged, and the part fish are also had to obvious teratogenesis.And olaquindox as egg, milk, honey etc. can enter the human foods chain, causes great harm to the human physical and mental health by contaminated animal body or relevant animal derived food.European Union in 2006 forbids that the olaquindox medicine is as feed addictive, requires must not detect in animal derived food; Japan forbids using olaquindox in poultry; " People's Republic of China's veterinary drug allusion quotation " regulation, olaquindox, as antivirus somatotropic agent, only for the growth promotion for the following pig of 35 kg, and is prevented and treated piglet yellow scour, dysentery characterized by white mucous stool, pig salmonella infection, off-drug periods 35 d; Forbid and surpass other animals such as pig more than 35 kg and fowl, fish in body weight.
The residual detection method of olaquindox mainly relies on thin-layered chromatography, high performance liquid chromatography, mass spectroscopy, high efficiency liquid phase-MS etc. at present, these methods often need the instrument of complex and expensive, and need, through loaded down with trivial details sample pre-treatments, to be difficult to meet quick, easy Site Detection requirement.The immune analysis technology has the advantages such as sensitive, quick, special, easy, is a kind of important trace detection technology grown up gradually in recent years, more and more in the application of residue of veterinary drug analysis field, has wide popularizing application prospect.
The colloid gold label immunoassay is a kind of novel analytical technology developed rapidly in recent years, be characterized in easy fast, cost is low, pollution-free, without training, be very suitable for Site Detection.Compare with ELISA, have sample pre-treatments simple, developing time short (3-5 min), and all pack are contained on a test strips, without using the advantage such as instrument, have wide market outlook and using value.Therefore, develop quick, sensitive, efficient olaquindox immunology detection test card for ensureing that the animal food safety tool is of great significance.
Summary of the invention
The technical matters that the utility model solves be to provide a kind of simple to operate, quick and precisely, highly sensitive, cost is low, good stability, can carry out the olaquindox immunology detection test card of batch detection.
The technical solution of the utility model is: a kind of immunology detection test card of olaquindox, it is characterized in that: described olaquindox immunology detection test card comprises plastic box body and is packaged in the test strips in plastic box body, the bottom of described test strips is supporting layer, on this supporting layer by the left-to-right closely connected sample pad that is attached with successively, gold labeling antibody pad, nitrocellulose filter and absorption pad, have well and view window on described plastic box body, the position of this well is corresponding with the sample pad position on test strips, view window is corresponding with the cellulose nitrate film location on test strips, the detection line (T line) of olaquindox-carrier protein couplet thing solution printing and the nature controlling line (C line) that sheep anti-mouse igg solution is printed are arranged on described nitrocellulose filter, between the two apart from 5 mm, the detection line (T line) that wherein olaquindox-carrier protein couplet thing solution is printed is positioned at the side near sample pad, be perfused with the anti-olaquindox monoclonal antibody of colloid gold label on described pad.
Supporting layer described in the utility model is to be coordinated the PVC plate made from stabilizing agent and auxiliary material by Corvic.Absorption pad described in the utility model is that the plant long fibre extremely strong by water-intake capacity is the pure white filter paper that starting material are made.Sample pad described in the utility model and golden labeling antibody pad are made by glass fibre cotton.The width that laminates of sample pad described in the utility model and golden labeling antibody pad is 2 mm, and the width that laminates of absorption pad and nitrocellulose filter is 2 mm.Sample pad described in the utility model, golden labeling antibody pad and absorption pad top are coated with rubber protective layer.Nitrocellulose filter described in the utility model two ends boundary has mark line.
The utlity model has following advantage: (1) is highly sensitive, high specificity.Olaquindox immunology detection test card is prepared from the monoclonal antibody of colloid gold label high-affinity, in the gold labeling antibody, gold grain combines by Van der Waals force between the charges of different polarity with antibody molecule, and collaurum is very little on specificity and the affinity impact of monoclonal antibody.Therefore, the quick detection test paper jig has higher sensitivity and specificity, and its detection sensitivity can reach 5 ng/ml;
(2) simple, convenient quick.While using the quick detection test paper card, without other any reagent, as long as the sample solution after processing is splashed in the test card well to 3 ~ 4 with dropper, 3 ~ 5 min get final product observations;
(3) testing result image, directly perceived.Test card is usingd red trace line " | " or " ‖ " positive and the negative marker as detection line, when nature controlling line (C line) shows a redness " | " trace on nitrocellulose filter, mean olaquindox residual content >=5 ng/ml in detected sample solution; When if two redness " ‖ " trace appears in nature controlling line on nitrocellulose filter (C line) and detection line (T line) simultaneously, mean olaquindox residual content<5 ng/ml in sample solution.Result is visual in image, accurately simple, is not easy to occur erroneous judgement;
(4) use of glued membrane can extend testing result observing time, test card good stability.This test card absorption of sample pad will detect solution and absorb fully, make it fully to react with golden labeling antibody on the coupling pad, can effectively reduce error rate; Can also prevent that introduced contaminants from disturbing, and affects the combination of golden labeling antibody and detectable antigens;
(5) low, the small investment of cost.Use the utility model test card, do not need separately to join complicated instrument and equipment and expensive reagent, Site Detection settles at one go, with low cost, instant effect;
(6) be easy to apply on a large scale.This test card is simple to operate, is applicable to different classes of librarian use, as laboratory detection, customs quarantine control, health supervision, breeding scale and individual production etc., has wide market outlook and larger economic benefit and social benefit.
The accompanying drawing explanation
Fig. 1 is structural representation of the present utility model; Fig. 2 is the side view of test strips in the utility model; Fig. 3 is the vertical view of test strips in the utility model.
Embodiment
Describe by reference to the accompanying drawings embodiment in detail.A kind of immunology detection test card of olaquindox, comprise plastic box body 1 and be packaged in the test strips 2 in plastic box body 1, the bottom of described test strips 2 is supporting layer 3, on this supporting layer 3 by the left-to-right closely connected sample pad 4 that is attached with successively, gold labeling antibody pad 5, nitrocellulose filter 6 and absorption pad 7, have well 8 and view window 9 on described plastic box body 1, the position of this well 8 is corresponding with sample pad 4 positions on test strips 2, view window 9 is corresponding with nitrocellulose filter 6 positions on test strips 2, detection line (the T line) 11 of olaquindox-carrier protein couplet thing solution printing and the nature controlling line (C line) 10 that sheep anti-mouse igg solution is printed are arranged on described nitrocellulose filter 6, between the two apart from 5 mm, the detection line (T line) 11 that wherein olaquindox-carrier protein couplet thing solution is printed is positioned at the side near sample pad 4, be perfused with the anti-olaquindox monoclonal antibody of colloid gold label on described pad 5.
Supporting layer 3 described in the utility model is to be coordinated the PVC plate made from stabilizing agent and auxiliary material by Corvic.Absorption pad 7 described in the utility model is that the plant long fibre extremely strong by water-intake capacity is the pure white filter paper that starting material are made.Sample pad 4 described in the utility model and golden labeling antibody pad 5 are made by glass fibre cotton.The width that laminates of sample pad 4 described in the utility model and golden labeling antibody pad 5 is 2 mm, and absorption pad 7 is 2 mm with the width that laminates of nitrocellulose filter 6.Sample pad 4 described in the utility model, golden labeling antibody pad 5 and absorption pad 7 tops are coated with rubber protective layer 12.Nitrocellulose filter 6 two ends boundarys described in the utility model have mark line 13.
The detection principle of test card described in the utility model:
Test card designs according to antigen-antibody competitive immunization chromatographic theory.After dripping sample liquid, under the capillary action of absorption pad and nitrocellulose filter, sample solution is migration upwards, and while arriving pad, golden labeling antibody is by dissolved.While in sample, containing olaquindox, they will with golden labeling antibody combination, and upwards migration together, arrive while being fixed with the detection line position of OLA-OVA, detectable antigens will be with the olaquindox competition in conjunction with antigen binding site limited on golden labeling antibody.In sample, OLA content is higher, and detectable antigens and golden labeling antibody are just fewer in conjunction with quantity, and the colour developing of T line is just more weak; When in sample, OLA is higher than certain numerical value (5 ng/ml), detectable antigens just can't with golden labeling antibody combination, the T line does not develop the color.Whether no matter in sample, have OLA to exist, excessive golden labeling antibody or detectable antigens all will be combined with two anti-GaMIgG with the bond of golden labeling antibody, at the C line, form redness.
The using method of test card described in the utility model:
1) sample pre-treatments:
The disposal route of tissue (pork liver, pork, fish, shrimp etc.) sample: take 2 ± 0.05 g homogenate tissues, add 10 ml anhydrous acetonitriles, 5 min that vibrate, above centrifugal 10 min of 4000 r/min under room temperature; Get supernatant 5 ml dries up under 50 ℃ of nitrogen or air; With the dry thing of 2 ml n-hexane dissolutions, the redissolution liquid diluted with 1 ml (2X concentrated redissolution liquid and deionized water 1:1 dilution) fully mixes 30 s; Above centrifugal 5 min of 4000 r/min; Getting 50 μ l is analyzed.
2) sample drips:
After test card is kept flat, with dropper, sample solution is splashed in the test card well to 3 ~ 4, after 3 ~ 5 min, get final product observations.
3) Analysis of test results:
It is negative: if, when on nitrocellulose filter, two redness " ‖ " trace appears in nature controlling line (C line) and detection line (T line), olaquindox residual content<5 ng/ml in the expression sample solution, be judged to feminine gender simultaneously.
Positive: if on nitrocellulose filter, nature controlling line (C line) shows a redness " | " trace, and detection line (T line) does not develop the color, and means olaquindox residual content >=5 ng/ml in detected sample solution, is judged to the positive.
Invalid: if on nitrocellulose filter, nature controlling line (C line) does not show red stripes, no matter whether detection line (T line) red trace occurs, and it is invalid that this test card all is judged to.
Claims (7)
1. the immunology detection test card of an olaquindox, it is characterized in that: described olaquindox immunology detection test card comprises plastic box body and is packaged in the test strips in plastic box body, the bottom of described test strips is supporting layer, on this supporting layer by the left-to-right closely connected sample pad that is attached with successively, gold labeling antibody pad, nitrocellulose filter and absorption pad, have well and view window on described plastic box body, the position of this well is corresponding with the sample pad position on test strips, view window is corresponding with the cellulose nitrate film location on test strips, the detection line of olaquindox-carrier protein couplet thing solution printing and the nature controlling line that sheep anti-mouse igg solution is printed are arranged on described nitrocellulose filter, between the two apart from 5 mm, the detection line that wherein olaquindox-carrier protein couplet thing solution is printed is positioned at the side near sample pad, be perfused with the anti-olaquindox monoclonal antibody of colloid gold label on described pad.
2. olaquindox immunology detection test card according to claim 1, it is characterized in that: described supporting layer is the PVC plate.
3. olaquindox immunology detection test card according to claim 1, it is characterized in that: described absorption pad is to be the pure white filter paper that starting material are made by the plant long fibre.
4. olaquindox immunology detection test card according to claim 1, it is characterized in that: described sample pad and golden labeling antibody pad are made by glass fibre cotton.
5. olaquindox immunology detection test card according to claim 1, it is characterized in that: the width that laminates of described sample pad and golden labeling antibody pad is 2 mm, the width that laminates of absorption pad and nitrocellulose filter is 2 mm.
6. olaquindox immunology detection test card according to claim 1, is characterized in that: be coated with rubber protective layer above described sample pad, golden labeling antibody pad and absorption pad.
7. olaquindox immunology detection test card according to claim 1, it is characterized in that: described nitrocellulose filter two ends boundary has mark line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320434942.1U CN203385729U (en) | 2013-07-19 | 2013-07-19 | Immunological test paper card for olaquindox |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320434942.1U CN203385729U (en) | 2013-07-19 | 2013-07-19 | Immunological test paper card for olaquindox |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203385729U true CN203385729U (en) | 2014-01-08 |
Family
ID=49874205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320434942.1U Expired - Lifetime CN203385729U (en) | 2013-07-19 | 2013-07-19 | Immunological test paper card for olaquindox |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203385729U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370848A (en) * | 2016-10-21 | 2017-02-01 | 北京康思润业生物技术有限公司 | Immune lateral chromatography detection system and preparation method thereof |
| CN106771161A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of olaquindox metabolite detection method and detection card |
| CN111089960A (en) * | 2020-01-13 | 2020-05-01 | 佛山职业技术学院 | Quinoethanol rapid detection device |
| CN111505271A (en) * | 2020-03-10 | 2020-08-07 | 玉林师范学院 | Preparation method of olaquindox rapid detection test strip |
-
2013
- 2013-07-19 CN CN201320434942.1U patent/CN203385729U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370848A (en) * | 2016-10-21 | 2017-02-01 | 北京康思润业生物技术有限公司 | Immune lateral chromatography detection system and preparation method thereof |
| CN106771161A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of olaquindox metabolite detection method and detection card |
| CN111089960A (en) * | 2020-01-13 | 2020-05-01 | 佛山职业技术学院 | Quinoethanol rapid detection device |
| CN111505271A (en) * | 2020-03-10 | 2020-08-07 | 玉林师范学院 | Preparation method of olaquindox rapid detection test strip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Surai et al. | Revisiting oxidative stress and the use of organic selenium in dairy cow nutrition | |
| Padalino et al. | Immunological, clinical, haematological and oxidative responses to long distance transportation in horses | |
| Idris et al. | Gastrointestinal nematode infections in German sheep | |
| Casella et al. | Influence of transportation on serum concentrations of acute phase proteins in horse | |
| CN203385729U (en) | Immunological test paper card for olaquindox | |
| CN204241487U (en) | The colloidal gold immune chromatography test card that a kind of Lomefloxacin and antibody thereof remain | |
| Bane et al. | Relationship between fumonisin contamination of feed and mystery swine disease: a case-control study | |
| CN103713131B (en) | Detection spectinomycin, streptomycin, gentamycin and the test strips of neomycin and method | |
| CN103499690B (en) | Olaquindox metabolite immunochromatographytest test paper card and preparation method thereof | |
| CN103743908A (en) | Neomycin semi-quantitative gold-labeled rapid detection kit, and detection method and application thereof | |
| Lee et al. | The potential of silage lactic acid bacteria-derived nano-selenium as a dietary supplement in sheep | |
| CN102967707A (en) | Triple immune colloidal gold rapid detection card and its preparation and use method | |
| CN103116031A (en) | Quick trenbolone residue detection test paper card and preparation method thereof | |
| Cusack et al. | The physiological and production effects of increased dietary intake of vitamins E and C in feedlot cattle challenged with bovine herpesvirus | |
| Noaman et al. | Copper, zinc, and iron concentrations in blood serum and diet of dairy cattle on semi-industrial farms in central Iran | |
| CN202794181U (en) | Test paper card for rapid detection of aflatoxin B1 residue | |
| CN204044161U (en) | Deoxynivalenol enol colloidal gold reagent plate in a kind of grain and oil of detection fast | |
| CN103257224A (en) | Tandem multiplex detection colloidal gold test strip | |
| CN103105491A (en) | Kit and method for detecting beta-lactam antibiotic and tetracycline antibiotic | |
| CN203385738U (en) | Colloidal gold immunochromatographic test paper card for diethylstilbestrol | |
| CN203385728U (en) | Malachite green residue test paper card | |
| CN103353524A (en) | Test strip for rapidly detecting microscale olaquindox | |
| CN103941009B (en) | ISG15 colloidal gold strip for cattle and sheep diagnosis of early gestation and preparation method thereof | |
| Mushi et al. | Detection of Mycoplasma gallisepticum and Mycoplasma synoviae antibodies in the sera of indigenous chickens by rapid serum agglutination test at Mmopane, Gaborone, Botswana | |
| Singh et al. | Effect of pre‐partum supplementation of vitamin E to Murrah buffaloes on immune functions and viability of calves |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140108 |