CN202453289U - Specific protein measuring device - Google Patents
Specific protein measuring device Download PDFInfo
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- CN202453289U CN202453289U CN2011202726946U CN201120272694U CN202453289U CN 202453289 U CN202453289 U CN 202453289U CN 2011202726946 U CN2011202726946 U CN 2011202726946U CN 201120272694 U CN201120272694 U CN 201120272694U CN 202453289 U CN202453289 U CN 202453289U
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Abstract
The utility model relates to the field of biochemical analytical instruments, in particular to a specific protein measuring device. The specific protein measuring device comprises two laser sources, two incident light paths, a reaction cup, two scattered light paths, two photoelectric detection units and a calculation display unit, and is characterized in that emitted lights (S1 and S2) of the laser sources (1 and 2) enter the reaction cup (5) vertically by the incident light paths (3 and 4) from surfaces in which a long edge (L) and a short edge (H) of the reaction cup (5) are positioned respectively; the emitted lights perform scattering and transmission in the reaction cup (5), and scattered light (S3) of the emitted light (S1) converges to a photoelectric detection unit (9) by a scattered light path (7); scattered light (S4) of the emitted light (S2) converges to a photoelectric detection unit (10) by a scattered light path (8); voltage signals outputted by the photoelectric detection units (9 and 10) are transmitted to a calculation display unit (11); and the calculation display unit (11) calculates and displays a measuring result.
Description
Technical field:
The utility model relates to the biochemical analyzer field, specifically is a kind of specific protein measurement mechanism.
Background technology:
The specific protein measuring instrument is a kind of human body fluid specified protein analysis on Content instrument that is used for measuring; It has utilized the principle of laser light scattering than opacimetry; Turbidity through solution after sample and the reagent reacting in the measurement reaction cup; Come the height of specified protein content in the working sample, the height of specified protein content has then reacted the corresponding state of patient body, for diagnosis provides effective reference date.Therefore, the specific protein measuring instrument is a kind of at the widely used biochemical analyzer of medical institutions, and the use amount of instrument is big; But because patient's the state of an illness is widely different, so the specified protein content difference is also very big, when the specified protein content that will detect is very big; Solution turbidity in the reaction cup is just high; The solution scattered light intensity that turbidity is high is just big, is easy to cause photodetector unit output saturated, the signal of distortion occurs; When the specified protein content that will detect was very little, the solution turbidity in the reaction cup was just low, the high solution scattered light intensity of turbidity just a little less than, photodetector unit output signal is just little, is difficult to detect.Therefore how to improve the sensitivity of detection, and effectively to remove distortion value be unusual important problem.
(CN 1224497A) is the most frequently used detection method to the patent of Beckman Instruments Inc. " nephelometer and nephometer combination ", but it does not propose a solution; The patent of Tianjin MD Pacific Technology Co., Ltd. " the analyser gauge head and the measuring cup of turbid detection of multispectral scattering and transmittance " (CN201673117U) has proposed to adopt multispectral method to measure; And measure transmission and scattered light simultaneously; Also not sensitivity to improve detecting, and effectively remove distortion value and propose a solution.
The utility model content:
Technical matters: the utility model problem to be solved just provides a kind of specific protein measurement mechanism, can improve the sensitivity of detection, effectively removes distortion value.
Technical scheme: what the specific protein device that the utility model adopts adopted is laser two-way scattering turbidimetry method, and implement device mainly is made up of two lasing light emitters, two input paths, reaction cup, two scattering light paths, two photodetector unit, calculation display unit.Specifically: reaction cup 5 cross sections are rectangles; Lasing light emitter 1 and 2 emission light S1 and S2; Timesharing is successively through input path 3 and 4; From the long limit L of reaction cup 5 and the vertical reaction cup 5 that gets into of face at minor face H place, scattering takes place for 5 li in reaction cup in emission light respectively, and the scattered light S3 of emission light S1 converges to photodetector unit 9 through scattering light path 7; The scattered light S4 of emission light S2 converges to photodetector unit 10 through scattering light path 8; The voltage signal of photodetector unit 9 and 10 outputs sends calculation display unit 11 to, and calculation display unit 11 calculates and the demonstration measurement result.
The cross section of reaction cup 5 is rectangles, and length is l between the inwall of its long limit L, and length is h between the inwall of minor face H, and both ratio ranges are: 2＜l/h＜4, and two relative planes at the place, long limit of reaction cup 5 are parallel to each other; Two relative planes at the minor face place of reaction cup 5 also are parallel to each other, and therefore, the light path of the sample solution generation scattering that two bundle incident light S1 and S2 and reaction cup are 5 li is just unequal; According to the principle of laser light scattering turbidimetry, the light path of laser in the sample solution of reaction cup is long more, and the quantity of the particle generation scattering that suspends in laser and the sample solution is just many more; Scattered light is just strong more, therefore, utilizes two length of sides of reaction cup unequal; Two scattered light intensities that do not wait have just been realized; The optical length of scattering takes place in the incident light S1 that gets into from minor face H, and the light intensity of scattered light S4 is just big, the sensitivity when this phenomenon can help to provide low concentration sample to measure; The incident light S2 that gets into from long limit L takes place that the light path of scattering is short, and the light intensity of scattered light S3 is just little, and this phenomenon can help to reduce high concentration sample scattered light intensity, when calculating, judges through calculating, and removes the distortion value of photodetector unit output.
(1) usually, V2 (i)＞V1 (i) at first compares two groups of sequential values one by one, if in certain same period, occur V2 (i)=＜V1 (i) situation, then V2 (i) is a distortion value, this sequence will abandon, and only leaves and takes V1 (i) sequence and calculates;
(2) calculate the rate of change of two column data respectively; Obtain corresponding two groups of speed sequential value: K1 (i) and K2 (i), find out the maximal value K2 (max) and the corresponding V2 (j) thereof of K2 (i) sequence, find out the value V1 (j) of j photodetector unit 9 outputs constantly again; If V2 (i)=＜V1 (i); Then V2 (i) is a distortion value, and this sequence will abandon, and only leaves and takes V1 (i) sequence and calculates;
(3) calculate the rate of change of two column data respectively, obtain corresponding two groups of speed sequential value: K1 (i) and K2 (i), find out the maximal value K1 (max) and the corresponding V1 (j) thereof of K1 (i) sequence; Find out the maximal value K2 (max) and the corresponding V2 (k) thereof of K2 (i) sequence; After the situation of getting rid of above-mentioned (2), compare j and k, if j＞k; Then leave and take V1 (i) sequential value, abandon V2 (i) sequential value; If j=＜k then leaves and takes V2 (i) sequential value, abandon V1 (i) sequential value.
(4) choose the ordered sequence value with said method after, compare according to the standard sequence value that 11 li of calculation display unit are deposited in advance, calculate measured value.
Beneficial effect: this specific protein measuring method and device can improve the sensitivity of detection, and effectively remove distortion value.
Description of drawings
Below in conjunction with accompanying drawing the utility model is described further.
Fig. 1 is that the system of the utility model implement device constitutes synoptic diagram.
Fig. 2 is a reaction cup front view in the utility model implement device.
Fig. 3 is a reaction cup side view in the utility model implement device.
Fig. 4 is a reaction cup B section sectional view in the utility model implement device.
Embodiment
Fig. 1 is that the system of the utility model implement device constitutes synoptic diagram.The specific protein measuring method that the utility model adopts is a kind of laser two-way scattering turbidimetry method, and implement device mainly is made up of two lasing light emitters, two input paths, a special reaction cup, two scattering light paths, two photodetector unit, calculation display unit.Specifically: reaction cup 5 cross sections are rectangles; Lasing light emitter 1 and 2 emission light S1 and S2, timesharing is successively through input path 3 and 4, the vertical reaction cup 5 that gets into of face that belongs to from the long limit L and the minor face H of reaction cup 5 respectively; Scattering takes place for 5 li in reaction cup in emission light; The scattered light S3 of emission light S1 converges to photodetector unit 9 through scattering light path 7, and transmitted light S5 gets into light and falls into 12, is absorbed; The scattered light S4 of emission light S2 converges to photodetector unit 10 through scattering light path 8, and transmitted light S6 gets into light and falls into 13, is absorbed; The voltage signal of photodetector unit 9 and 10 outputs sends calculation display unit 11 to, and calculation display unit 11 calculates and the demonstration measurement result.
Two lasing light emitters 1 are that the same same model semiconductor laser of performance and driving circuit thereof are formed with 2; Article two, input path 3 also is the same with 4 parameter, performance; Article two, scattering light path 7 also is the same with 8 parameter, performance; Two photodetector unit 9 and 10 material, technology, performance are just the same.Special reaction cup 5 is to be placed in the calibration cell 6 when detecting, and the different sole cause of voltage signal of photodetector unit 9 and 10 output is that two incident light S1 are different with the light path of S2 entering reaction cup 5 and sample solution generation scattering, in the present embodiment; The long 9mm of the inwall on the long limit of reaction cup 5, the long 3mm of the inwall of minor face is according to the principle of laser light scattering turbidimetry; The light path of laser in the sample solution of reaction cup is long more, and the quantity of the particle generation scattering that suspends in laser and the sample solution is just many more, and scattered light is just strong more; Therefore; Utilize two length of sides of reaction cup unequal, just realized two scattered light intensities that do not wait, the optical length of scattering takes place from the incident light S2 of minor face H entering; The light intensity of scattered light S4 is just big, the sensitivity when this phenomenon can help to provide low concentration sample to measure; The light path weak point of scattering takes place from the incident light S1 of long limit L entering; The light intensity of scattered light S2 is just little; Scattered light intensity when this phenomenon can help to reduce the measurement of high concentration sample helps calculation display unit 11 when calculating, to carry out computational discrimination, removes the distortion value of photodetector unit 10 outputs.
The emission light intensity of two lasing light emitters equates in present embodiment, and centre wavelength all is 635nm, and emissive power is 1mw, and its driving circuit adopts the constant voltage driving mode, also can select current constant mode for use.
Because semiconductor laser is a kind of pointolite, emission light S1 and S2 must be collimated into approximate directional light through input path 3 and 4.Reaction cup 5 is to adopt the transparent plastics of optical grade to process, and reaction cup 5 is to be placed in the calibration cell 6 when detecting, and the control temperature of calibration cell 6 is near the human body normal body temperature.The performance of two photodetector unit is the same; Photodetector unit 9 and 10 all adopts the same model silicon photocell (model: BPW34) as accepting sensor of VISHAY company; Resistance in the sensor modulate circuit all adopts the resistance of per mille precision, has guaranteed the unanimity of all photodetector unit performances.The diameter of scattering light path 7 is 2mm in the examples of implementation.
Fig. 2 is the reaction cup synoptic diagram.Long 9mm between the inwall on the long limit of reaction cup 5, long 3mm between the inwall of minor face, wall thickness 1mm, reaction cup length limit ratio equals 3.The whole high 47mm of reaction cup 5.
Claims (4)
1. a specific protein measurement mechanism mainly is made up of two lasing light emitters, two input paths, reaction cup, two scattering light paths, two photodetector unit, calculation display unit; It is characterized in that: the emission light of two lasing light emitters; Respectively through two input paths; The vertical reaction cup that gets into of face from the long limit of reaction cup with the minor face place; Article two, scattering takes place in emission light in reaction cup, and two scattered lights that produced converge to corresponding photodetector unit respectively then respectively through two scattering light paths; The voltage signal of two photodetector unit output sends the calculation display unit to, and the calculation display unit calculates and shows measurement result.
2. specific protein measurement mechanism according to claim 1 is characterized in that: the emission light intensity of two lasing light emitters equates.
3. specific protein measurement mechanism according to claim 1 is characterized in that: the reaction cup cross section is a rectangle, and the inwall length on its long limit is l, and the inwall length of minor face is h, and both ratio ranges are: 2＜l/h＜4.
4. specific protein measurement mechanism according to claim 1 is characterized in that: the performance of two photodetector unit is the same.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202726946U CN202453289U (en) | 2011-07-29 | 2011-07-29 | Specific protein measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202726946U CN202453289U (en) | 2011-07-29 | 2011-07-29 | Specific protein measuring device |
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| CN202453289U true CN202453289U (en) | 2012-09-26 |
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| CN2011202726946U Expired - Lifetime CN202453289U (en) | 2011-07-29 | 2011-07-29 | Specific protein measuring device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288581A (en) * | 2011-07-29 | 2011-12-21 | 南京诺尔曼生物技术有限公司 | Specific protein measuring method and device |
| WO2015043030A1 (en) * | 2013-09-30 | 2015-04-02 | 江苏英诺华医疗技术有限公司 | Multi-detection position photoelectric detection device |
-
2011
- 2011-07-29 CN CN2011202726946U patent/CN202453289U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288581A (en) * | 2011-07-29 | 2011-12-21 | 南京诺尔曼生物技术有限公司 | Specific protein measuring method and device |
| CN102288581B (en) * | 2011-07-29 | 2016-04-27 | 南京诺尔曼生物技术有限公司 | A kind of specific protein measuring method and device |
| WO2015043030A1 (en) * | 2013-09-30 | 2015-04-02 | 江苏英诺华医疗技术有限公司 | Multi-detection position photoelectric detection device |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 210031 building E, building 10, Spark Road, No. four, Spark Road, Nanjing hi tech Development Zone, Jiangsu, China Patentee after: Nanjing Norman Biological Technology Co., Ltd. Address before: 5, 210029 building, 66-1 software building, Majiagou industrial district, Nanjing District, Yuhuatai, Jiangsu Patentee before: Nanjing Norman Biological Technology Co., Ltd. |
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| CX01 | Expiry of patent term |
Granted publication date: 20120926 |
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| CX01 | Expiry of patent term |