CN113607796A - Microfluid flow/flow rate and component cooperative detection device and application thereof - Google Patents
Microfluid flow/flow rate and component cooperative detection device and application thereof Download PDFInfo
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Abstract
The invention discloses a micro-fluid flow/flow rate and component cooperative detection device and application thereof, wherein the same detection device comprises a substrate, a micro-fluid channel layer and a cover plate; the device comprises a substrate, a liquid channel layer, a liquid inlet, a liquid outlet, a detection device and an electrochemical detection unit, wherein the substrate is provided with a laser-induced graphene pattern, the laser-induced graphene pattern is positioned on a projection surface of the liquid channel layer, the liquid inlet and the liquid outlet are arranged on the detection device and communicated with the liquid channel of the liquid channel layer, and the cooperative detection device is also provided with the electrochemical detection unit for detecting liquid components in the liquid channel. The invention utilizes the color change displayed by the LIG substrate in the liquid and air environment to determine the position of the liquid, further obtains the volume of the liquid and calculates the flow rate of the liquid in unit time, the electrochemical detection unit can acquire the concentration signals of the components in the detected liquid in the liquid channel in real time and calculate the data of the concentration of the corresponding components in the liquid along with the change of time, thereby establishing the coupling relation between the flow/flow rate of the liquid and the concentration change of various components in the liquid.
Description
Technical Field
The invention belongs to the technical field of microfluidic flow velocity measurement and component analysis, and particularly relates to a microfluidic flow/flow velocity and component cooperative detection device and application thereof.
Background
In a microfluidic electrochemical detection system, the improvement of the fluid flow rate can effectively enhance the mass transfer of a target detection object to the surface of an electrochemical electrode and improve the detection sensitivity of an electrochemical sensor. Accurate measurement of the flow rate and flow rate of the microfluid is a necessary condition to ensure the detection accuracy of the electrochemical sensor.
In addition, sweat contains electrolytes, metabolites, amino acids, proteins, hormones and other markers related to human conditions and health, which are equivalent to blood, epidermal microfluidics is coupled with a sweat sensing technology, molecular level data related to the physical conditions can be continuously acquired on line, in a non-invasive manner and in real time, and the sweat is a hot research field of current intelligent wearable equipment. The concentration of the health markers in sweat is related to the sweating rate, for example, the concentration of sodium ions and chloride ions in sweat increases with the increase of the sweating rate, the concentration of potassium ions and calcium ions decreases with the increase of the sweating rate, the pH is acidic (pH 3.5-6.0) at low sweating rate, the pH of sweat is slightly basic (pH 7.0-8.5) at high sweating rate, the concentration of lactic acid and urea is higher at low sweating rate, and the concentration of lactic acid and urea gradually decreases with the increase of the sweating rate, and the like. Therefore, in studying the effect of sex, age, season, disease or drug variables on sweat composition, one must consider the dependence of sweat composition on sweat rate, otherwise changes in sweat rate may mask or exaggerate changes in sweat composition caused by other factors to be examined. Therefore, simultaneous detection of fluid flow rate and chemical composition, compensation of the rate at which the composition is detected, is necessary.
In the current stage, the measurement of the flow rate and the components of the fluid in the microfluidic system is mainly realized by two technologies, namely an optical colorimetry and an electrochemical method, wherein the colorimetry flow rate/flow measurement is mainly realized by placing a water-soluble pigment near the inlet of a micro-channel to dye the fluid, and people can very easily distinguish the front end of the fluid and obtain the volume and the flow rate of the fluid in the forward flowing process. Colorimetric chemical composition measurements are primarily based on the occurrence of a reaction between the analyte and the label resulting in a color change, wherein there is a direct relationship between the color change and the analyte concentration, and the concentration of the analyte is obtained by analyzing the displayed color. The colorimetric method is simple and easy to realize, and samples for flow rate detection and measurement of various components cannot flow through the same microchannel and are all carried out through separate channels, so that mutual interference between dyed fluids is avoided, and sample pollution cannot be measured. This does not really establish a coupling relationship between the fluid flow rate and the chemical composition. The coupling can be achieved by using electrochemical sensing technology to enable the fluid flow rate/flow and the chemical components to be measured in a channel without mutual interference. However, it requires a specific signal detection, acquisition, amplification, conversion, processing and transmission system, which increases the difficulty and technical threshold of implementation compared with the colorimetric method.
In view of the above, in the field of microfluidic analysis, especially in the field of wearable bio-fluid sensing, there is a need for a detection system that can perform cooperative detection of fluid flow/velocity and components in one channel and has a simple implementation scheme.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a micro-fluid flow/flow rate and component cooperative detection device and application thereof.
The invention is realized by the following technical scheme:
a micro-fluid flow/flow rate and component cooperative detection device comprises a substrate, a micro-fluid channel layer and a cover plate; the substrate is positioned at the bottom side of the micro-flow channel layer, the cover plate is positioned at the upper side of the micro-flow channel layer, and a laser-induced graphene pattern is arranged on the substrate and positioned on the projection surface of the liquid channel of the micro-flow channel layer; a liquid inlet and a liquid outlet which are communicated with the liquid channel of the micro-flow channel layer are arranged on the detection device, and an electrochemical detection unit for detecting the liquid component in the liquid channel is also arranged on the cooperative detection device.
In the above technical solution, a scale is disposed on the cooperative detection apparatus along the liquid channel, and the scale is preferably disposed on the substrate, and may also be disposed on the micro-channel layer or the cover plate.
In the technical scheme, after the transparent liquid flows into the liquid channel of the micro-channel layer, the optical characteristics above the laser-induced graphene pattern are changed, so that distinguishable obvious color changes exist in the liquid-filled liquid channel part and the liquid-unfilled liquid channel part, the position of the liquid can be visually observed, and the liquid flow rate and the flow rate can be calculated according to the time interval.
In the above technical solution, the laser-induced graphene pattern is directly generated on the substrate by a carbon dioxide laser-induced method, and the substrate material is one of polyimide, polyetherimide, leaves, wood, or other carbon source substances capable of being ablated into graphene.
In the above technical solution, the sheet resistance of the laser-induced graphene pattern is preferably in the range of 5-1000 Ω/sq.
In the technical scheme, the combination of the substrate, the micro-flow channel layer and the cover plate adopts a mode of adhesive tape bonding or hot-press bonding; the micro-channel layer and the cover plate are made of one of glass, silicon rubber and transparent organic polymer materials.
In the technical scheme, the refractive indexes of the materials of the micro-flow channel layer and the cover plate are close to the refractive index of the measured liquid, so that the loss of light rays is reduced as much as possible when the light rays are transmitted to the outside of the micro-flow channel layer and the cover plate from the laser-induced graphene pattern, the color change of the laser-induced graphene pattern in the liquid environment is observed more easily, and the flow speed/flow detection is facilitated.
In the technical scheme, the number of the electrochemical detection units can be one or more, the electrochemical detection units can be constructed on any one of the upper side and the lower side of the micro-flow channel layer, and multi-parameter measurement can also be carried out on the upper side and the lower side of the micro-flow channel layer in a multi-point arrangement manner; the electrochemical detection unit can also adopt an external electrochemical sensor chip, the electrochemical sensor chip is inserted into the cooperative detection device, and the electrochemical sensor chip can be arranged at any position of the liquid channel.
In the technical scheme, the flow image of the detected liquid in the cooperative detection device is acquired in real time through the image acquisition device, and the acquired image is processed through the processor to automatically obtain a flow/flow speed real-time result.
The method for detecting the coupling relation between the sweat flow/flow rate and the Na + concentration in the sweat by using the synergistic detection device comprises the following steps:
an electrochemical detection unit for detecting Na + concentration signals is arranged in the cooperative detection device, a liquid inlet is arranged on a substrate, a liquid outlet is arranged on a cover plate, the substrate of the cooperative detection device faces towards the skin of a human body and is pasted on the surface of the skin of the human body, sweat generated by the human body enters a liquid channel from the liquid inlet of the cooperative detection device, the liquid pressure of the liquid inlet is increased along with continuous overflow of the sweat from the skin, the sweat is promoted to flow towards the liquid outlet along the liquid channel, after the sweat flows into the liquid channel, the original LIG and the overall optical characteristics of air above the LIG are changed, the refraction and scattering characteristics of natural light and the like of the LIG and the air are changed, so that obvious color changes which can be identified by human eyes exist in the liquid filling channel and the air filling channel, the position of the sweat can be visually observed, and under the condition that the size of the cross section of the liquid channel is known, parameters such as sweat flow rate and flow rate can be calculated according to the time interval; when the sweat flow/flow speed is detected, an electrochemical detection unit in the cooperative detection device can collect Na + concentration signals in the sweat in the liquid channel in real time, and the Na + concentration data of the sweat along with time change is calculated according to the signals, so that the coupling relation between the sweat flow/flow speed and the Na + concentration in the sweat is established.
The method for detecting the coupling relation between the flow rate/flow rate of the sweat and the concentration of various components in the sweat by using the cooperative detection device comprises the following steps:
a plurality of electrochemical detection units are arranged in the cooperative detection device and are used for detecting concentration signals of different components in sweat; the liquid inlet is arranged on the substrate, the liquid outlet is arranged on the cover plate, the substrate of the cooperative detection device faces the skin of a human body and is pasted on the surface of the skin of the human body, sweat generated by the human body enters the liquid channel from the liquid inlet of the cooperative detection device, the liquid pressure of the liquid inlet is increased along with the continuous overflow of the sweat from the skin, the flow of the sweat to the liquid outlet along the liquid channel is promoted, after the sweat flows into the liquid channel, the original LIG and the general optical characteristics of air above are changed, the refraction and scattering characteristics of natural light and the like are changed, so that the liquid filling channel and the air filling channel have obvious color changes which can be recognized by eyes, the positions of the sweat can be visually observed, and parameters such as sweat flow rate, flow rate and the like can be calculated according to time intervals under the condition that the size of the cross section of the liquid channel is known; when the sweat flow/flow rate is detected, the electrochemical detection unit in the cooperative detection device can collect concentration signals of components in the sweat in the liquid channel in real time, and the concentration data of the corresponding components in the sweat along with time change is calculated according to the signals, so that the coupling relation between the sweat flow/flow rate and the concentration change of various components in the sweat is established.
The invention has the advantages and beneficial effects that:
1. the detection of the flow velocity/flow rate of the fluid in the micro-channel is realized by the fact that the LIG changes when meeting water, the LIG is manufactured in a mode that carbon dioxide CO2 laser scans polymers (such as polyimide) under the air and room temperature environment conditions, the manufacturing process is simple, the cost is low, and batch production can be achieved. Through the scale resolution ratio of improving the scale and the resolution ratio of record time, can reach higher detection precision, measurement scheme simple and practical.
2. Compared with the method for testing the flow velocity/flow of the fluid by using an optical method for dyeing microfluid by using dye, the method utilizes the color change of the LIG substrate micro-channel system under the liquid and air environments to determine the position of the liquid, further obtains the volume of the liquid and calculates the flow rate of the liquid in unit time, so that the device has no pollution to the sample.
3. Because the flow velocity/flow test process can not cause pollution to microfluid, therefore this device can be integrated the electrochemical sensor that carries out sample component analysis to microfluid flow velocity/flow detection passageway in, though the same fluid channel of two kinds of sensors and the sample of surveying based on optics principle and electrochemistry principle coexists, does not have mutual influence between nevertheless, realizes mutual noninterference's collaborative test, can carry out more accurate analysis to microfluid. Meanwhile, a plurality of electrochemical sensors can be integrated at different positions of the micro-channel for component analysis without remarkably increasing the size of the system, and the development of a multifunctional system is facilitated.
Drawings
FIG. 1 is a schematic diagram of the structure of the cooperative detection apparatus of the present invention;
fig. 2.1 is a microscopic image of the LIG pattern of the present invention;
FIG. 2.2 is a microscopic image of the LIG pattern of the present invention;
FIG. 3 is a diagram showing the flow state of a microfluid in the cooperative detection apparatus of the present invention;
FIGS. 4.1-4.5 are schematic illustrations of the arrangement of electrochemical detection cells;
FIG. 5 is a schematic view of a configuration in which a plurality of electrochemical detection units are arranged in the cooperative detection apparatus;
FIG. 6.1 is a diagram of a three-electrode configuration for different electrode shapes;
FIG. 6.2 is a schematic of an electrochemical sensor based on a two or three electrode configuration;
fig. 6.3 is a schematic cross-sectional view and specific materials of representative working and reference electrodes for component measurement.
For a person skilled in the art, other relevant figures can be obtained from the above figures without inventive effort.
Detailed Description
In order to make the technical solution of the present invention better understood, the technical solution of the present invention is further described below with reference to specific examples.
Example one
A device for detecting the flow rate and the composition of a micro-fluid cooperatively, which is shown in figure 1, comprises a substrate 1, a micro-fluid channel layer 2 and a cover plate 3.
The substrate 1 is located on the bottom side of the micro-channel layer 2, the cover plate 3 is located on the upper side of the micro-channel layer 2, a liquid channel 2-1 is arranged in the micro-channel layer 2, a Laser Induced Graphene (LIG) pattern 1-1 is arranged on the substrate 1, and the LIG pattern 1-1 is located on a projection plane of the liquid channel 2-1 of the micro-channel layer.
A liquid inlet 1-2 is arranged on the substrate 1, a liquid outlet 3-1 is arranged on the cover plate 3, the liquid inlet 1-2 is positioned at one end of the liquid channel 2-1, the liquid outlet 3-1 is positioned at the other end of the liquid channel 2-1, and the liquid inlet 1-2 and the liquid outlet 3-1 are communicated with the liquid channel 2-1; thus, when the device is attached to the biological skin surface, sweat can flow into the liquid channel 2-1 of the micro-channel layer 2 from the liquid inlet 1-2 on the substrate 1, and as the sweat continuously overflows from the skin, the liquid pressure of the liquid inlet 1-2 is increased, so that the sweat is promoted to flow along the liquid channel 2-1 to the liquid outlet 3-1. Referring to fig. 3, after sweat flows into the liquid channel 2-1, the original LIG and the general optical characteristics of the air above the LIG are changed, the refraction and scattering characteristics of natural light are changed, so that the liquid filling channel and the air filling channel have obvious color changes recognizable by human eyes, and the position of the sweat can be visually observed (a scale is arranged on the cooperative detection device along the liquid channel, referring to fig. 1, the scale is preferably arranged on the substrate 1, and can also be arranged on the micro-channel layer 2 or the cover plate 3), and parameters such as sweat flow rate and flow rate can be calculated according to time intervals under the condition that the size of the cross section of the liquid channel 2-1 is known.
The LIG pattern 1-1 is directly generated on a substrate by a carbon dioxide (CO2) laser induction method, wherein the substrate material may be polyimide, polyetherimide, tree leaves, wood, etc. The LIG pattern may cover the projected area of the entire liquid channel or may be distributed only in a part of the projected area of the liquid channel, and the width W (LIG) of the LIG pattern is preferably in the range of W (LIG) or more than W (channel) as compared with the width W (channel) of the liquid channel, and the preferable size range is W (LIG) or more than 0.3W (channel), under which condition the color difference between the transparent liquid-filled channel and the air-filled channel is very significant.
The LIG used is in a porous structure (fig. 2.1 and 2.2), the pore structure and porosity of the LIG are closely related to the color (black and white) of the LIG, compared with the LIG in the porous structure shown in fig. 2.2, the LIG in fig. 2.1 is white to a certain extent, which is the color of the LIG after air is coupled with the graphene layer, the porosity of the LIG is directly related to the sheet resistance (sheet resistance), and the sheet resistance of the LIG is preferably selected in the range of 5-1000 Ω/sq for obtaining excellent flow rate/flow velocity measurement effect.
The combination of the substrate, the micro-flow channel layer and the cover plate can adopt the modes of adhesive tape adhesion, hot-press bonding and the like, and the materials of the micro-flow channel layer and the cover plate are selected as follows: glass, silicone rubber, organic polymer materials (e.g., polymethyl methacrylate PMMA, polyethylene terephthalate PET), and the like. When the refractive indexes of the materials of the micro-channel layer and the cover plate are close to the refractive index of the measured liquid (for example, the refractive index of water is about 1.3, and the refractive indexes of the materials selected for the micro-channel layer and the cover plate are between 1.1 and 1.5), the loss of light transmitted from the LIG pattern to the outside is low, the color change of the LIG pattern in the liquid environment is more easily observed, and the flow rate/flow detection is facilitated.
The cooperative detection device is also provided with an electrochemical detection unit for detecting liquid components in the liquid channel 2-1, the number of the electrochemical detection units can be one or more (see figure 1, 3 electrochemical detection units 3, 4 and 5 are arranged in figure 1), the electrochemical detection unit can be constructed on any one of the upper side and the lower side of the micro-channel layer (figure 4.1-4.5), and also can be arranged at multiple points on the upper side and the lower side of the micro-channel layer for multi-parameter measurement (figure 5), and the electrochemical detection unit can be constructed on a film layer forming a closed fluid channel no matter which side the electrochemical detection unit is arranged on, such as the substrate and/or the cover plate on the upper side and the lower side of the micro-channel layer (as shown in figures 4.1, 4.2 and 4.3, the electrochemical detection units are respectively positioned on the substrate and/or the cover plate on the upper side and the lower side of the micro-channel layer); the electrochemical detection unit can also adopt an external electrochemical sensor chip, the electrochemical sensor chip is inserted into the cooperative detection device (as shown in fig. 4.4 and 4.5), the electrochemical sensor chip can be arranged at any position of the liquid channel 2-1, including the front end, the middle or the rear end of the LIG pattern, on the trunk of the liquid channel (fig. 4.2-4.4) and at the side branch (fig. 4.1).
Referring to fig. 6.1-6.3, the electrochemical sensor chip is usually integrated on the same substrate in a two-electrode (working, comparison/reference) or three-electrode (working, comparison, reference) configuration, and can be a commercialized electrochemical sensor chip or a self-designed and constructed as required. The shape and layout of the three electrodes can be based on the general rule of the electrochemical sensor chip, but not limited to the structure shown in fig. 3, the materials of the working electrode and the comparison electrode include carbon, gold, platinum metal and the like, the reference electrode can be made of the same material as the working electrode and the comparison electrode, and can also be a silver/silver chloride Ag/AgCl and other classical reference electrodes, the substrate can be PET, PI and other flexible materials, and can also be glass and other hard materials. In order to realize detection selectivity, sensitivity and stability, the electrode can be modified, such as ion selective membrane modification, nano particle modification, enzyme modification and the like. The electrochemical sensor is sized as required.
Example two
On the basis of the first embodiment, the cooperative detection device can be further photographed through the image acquisition device, and the obtained image is further optimized through image processing, so that the degree of distinction between the liquid filling channel and the air filling channel can be better distinguished. Furthermore, the acquired images can be processed by the processor to automatically obtain the flow/flow rate real-time result.
EXAMPLE III
The method for detecting the coupling relation between the sweat flow/flow rate and the Na + concentration in the sweat by using the synergistic detection device comprises the following steps:
an electrochemical detection unit for detecting Na + concentration signals is arranged in the cooperative detection device, and Na of the electrochemical detection unit+The selective working electrode and the reference electrode are both manufactured on the LIG substrate 1, a Na + selective membrane is modified at the working electrode, a layer of Ag/AgCl membrane is firstly coated at the reference electrode, and then polyvinyl butyral PVB/sodium chloride NaCl/methanol solution is utilized for modification, so that the reference electrode with stable potential is obtained.
The cooperative detection device is pasted on the surface of the skin of a human body, sweat generated by the human body enters the liquid channel 2-1 from the liquid inlet 1-2 of the cooperative detection device, as the sweat continuously overflows from the skin, the liquid pressure of the liquid inlet 1-2 is increased, the sweat is promoted to flow along the liquid channel 2-1 to the liquid outlet 3-1, after sweat flows into the liquid channel 2-1, the original LIG and the overall optical characteristics of the air above are changed, the refraction and scattering characteristics of natural light are changed, so that obvious color changes which can be identified by human eyes exist in the liquid filling channel and the air filling channel, the position of sweat can be visually observed, and parameters such as sweat flow, flow rate and the like can be calculated according to time intervals under the condition that the size of the cross section of the liquid channel 2-1 is known; when the sweat flow/flow speed is detected, an electrochemical detection unit in the cooperative detection device can collect Na + concentration signals in the sweat in the liquid channel 2-1 in real time, and the Na + concentration data of the sweat along with time change is calculated according to the signals, so that the coupling relation between the sweat flow/flow speed and the Na + concentration in the sweat is established. The specific ion concentration corresponding to the electrochemical detection signal is obtained through the flow velocity, the influence of the flow velocity on the electrochemical detection sensitivity is eliminated, the rate compensation during component detection is realized, and the misjudgment of health detection and analysis caused by neglecting the dependence of sweat components on the flow velocity is avoided.
Example four
The method for detecting the coupling relation between the sweat flow/flow rate and the Na +, K + and Cl-concentration in the sweat by using the cooperative detection device comprises the following steps:
the cooperative detection device is provided with a first electrochemical detection unit for detecting a Na + concentration signal, a second electrochemical detection unit for detecting a K + concentration signal and a third electrochemical detection unit for detecting a Cl-concentration signal.
The cooperative detection device is pasted on the surface of the skin of a human body, sweat generated by the human body enters the liquid channel 2-1 from the liquid inlet 1-2 of the cooperative detection device, as the sweat continuously overflows from the skin, the liquid pressure of the liquid inlet 1-2 is increased, the sweat is promoted to flow along the liquid channel 2-1 to the liquid outlet 3-1, after sweat flows into the liquid channel 2-1, the original LIG and the overall optical characteristics of the air above are changed, the refraction and scattering characteristics of natural light are changed, so that obvious color changes which can be identified by human eyes exist in the liquid filling channel and the air filling channel, the position of sweat can be visually observed, and parameters such as sweat flow, flow rate and the like can be calculated according to time intervals under the condition that the size of the cross section of the liquid channel 2-1 is known; when the sweat flow/flow rate is detected, a first electrochemical detection unit in the cooperative detection device can collect Na + concentration signals in sweat in the liquid channel 2-1 in real time, a second electrochemical detection unit can collect K + concentration signals in sweat in the liquid channel 2-1 in real time, a third electrochemical detection unit can collect Cl-concentration signals in sweat in the liquid channel 2-1 in real time, and data of Na +, K + and Cl-concentration of the sweat along with time change are calculated according to the signals, so that the coupling relation between the sweat flow/flow rate and the Na +, K + and Cl-concentration of the sweat is established.
EXAMPLE five
The microfluidic flow/flow rate and component cooperative detection device is not limited to sweat detection, and can also be applied to flow/flow rate and component detection analysis of other transparent liquids. The liquid inlet and the liquid outlet of the same detection device can be arranged on the cover plate, and when the liquid detector is used, liquid to be detected can be injected into the liquid through the liquid inlet of the same detection device through the injector, so that flow/flow speed and component detection can be carried out.
The invention has been described in an illustrative manner, and it is to be understood that any simple variations, modifications or other equivalent changes which can be made by one skilled in the art without departing from the spirit of the invention fall within the scope of the invention.
Claims (10)
1. A microfluidic flow/velocity and composition cooperative detection apparatus, comprising: comprises a substrate, a micro-flow channel layer and a cover plate; the substrate is positioned at the bottom side of the micro-flow channel layer, the cover plate is positioned at the upper side of the micro-flow channel layer, and a laser-induced graphene pattern is arranged on the substrate and positioned on the projection surface of the liquid channel of the micro-flow channel layer; a liquid inlet and a liquid outlet which are communicated with the liquid channel of the micro-flow channel layer are arranged on the detection device, and an electrochemical detection unit for detecting the liquid component in the liquid channel is also arranged on the cooperative detection device.
2. The cooperative detection apparatus according to claim 1, characterized in that: and a scale is arranged on the cooperative detection device along the liquid channel.
3. The cooperative detection apparatus according to claim 1, characterized in that: after the transparent liquid flows into the liquid channel of the micro-channel layer, due to the fact that the optical characteristics above the laser-induced graphene pattern are changed, the liquid-filled liquid channel part and the liquid-unfilled liquid channel part have distinguishable obvious color changes, the position of the liquid can be visually observed, and the liquid flow rate and the flow rate can be calculated according to the time interval.
4. The cooperative detection apparatus according to claim 1, characterized in that: the laser-induced graphene pattern is directly generated on the substrate by a carbon dioxide laser-induced method, and the substrate material is one of polyimide, polyetherimide, tree leaves, wood or other carbon source substances capable of being ablated into graphene.
5. The cooperative detection apparatus according to claim 1, characterized in that: the sheet resistance of the laser-induced graphene pattern is preferably in the range of 5-1000 Ω/sq.
6. The cooperative detection apparatus according to claim 1, characterized in that: the combination of the substrate, the micro-flow channel layer and the cover plate adopts a mode of adhesive tape adhesion or hot-press bonding; the micro-channel layer and the cover plate are made of one of glass, silicon rubber and transparent organic polymer materials.
7. The cooperative detection apparatus according to claim 1, characterized in that: the refractive indexes of materials of the micro-channel layer and the cover plate are close to that of the measured liquid, so that loss of light rays is reduced as much as possible when the light rays are transmitted to the outside of the micro-channel layer and the cover plate from the laser-induced graphene pattern, the color change of the laser-induced graphene pattern in a liquid environment is observed more easily, and flow speed/flow detection is facilitated.
8. The cooperative detection apparatus according to claim 1, characterized in that: the number of the electrochemical detection units can be one or more, the electrochemical detection units can be constructed on any one of the upper side and the lower side of the micro-flow channel layer, and multi-parameter measurement can also be carried out on the upper side and the lower side of the micro-flow channel layer in a multi-point arrangement manner; the electrochemical detection unit can also adopt an external electrochemical sensor chip, the electrochemical sensor chip is inserted into the cooperative detection device, and the electrochemical sensor chip can be arranged at any position of the liquid channel.
9. A method for detecting a coupling between sweat flow/velocity and Na + concentration in sweat using the cooperative detection apparatus of any of claims 1-8 as follows:
an electrochemical detection unit for detecting Na + concentration signals is arranged in the cooperative detection device, a liquid inlet is arranged on a substrate, a liquid outlet is arranged on a cover plate, the substrate of the cooperative detection device faces towards the skin of a human body and is pasted on the surface of the skin of the human body, sweat generated by the human body enters a liquid channel from the liquid inlet of the cooperative detection device, the liquid pressure of the liquid inlet is increased along with continuous overflow of the sweat from the skin, the sweat is promoted to flow towards the liquid outlet along the liquid channel, after the sweat flows into the liquid channel, the original LIG and the overall optical characteristics of air above the LIG are changed, the refraction and scattering characteristics of natural light and the like of the LIG and the air are changed, so that obvious color changes which can be identified by human eyes exist in the liquid filling channel and the air filling channel, the position of the sweat can be visually observed, and under the condition that the size of the cross section of the liquid channel is known, parameters such as sweat flow rate and flow rate can be calculated according to the time interval; when the sweat flow/flow speed is detected, an electrochemical detection unit in the cooperative detection device can collect Na + concentration signals in the sweat in the liquid channel in real time, and the Na + concentration data of the sweat along with time change is calculated according to the signals, so that the coupling relation between the sweat flow/flow speed and the Na + concentration in the sweat is established.
10. A method for detecting a coupling relationship between sweat flow/velocity and concentration of a plurality of components in sweat using the cooperative detection device of any one of claims 1 to 8 as follows:
a plurality of electrochemical detection units are arranged in the cooperative detection device and are used for detecting concentration signals of different components in sweat; the liquid inlet is arranged on the substrate, the liquid outlet is arranged on the cover plate, the substrate of the cooperative detection device faces the skin of a human body and is pasted on the surface of the skin of the human body, sweat generated by the human body enters the liquid channel from the liquid inlet of the cooperative detection device, the liquid pressure of the liquid inlet is increased along with the continuous overflow of the sweat from the skin, the flow of the sweat to the liquid outlet along the liquid channel is promoted, after the sweat flows into the liquid channel, the original LIG and the general optical characteristics of air above are changed, the refraction and scattering characteristics of natural light and the like are changed, so that the liquid filling channel and the air filling channel have obvious color changes which can be recognized by eyes, the positions of the sweat can be visually observed, and parameters such as sweat flow rate, flow rate and the like can be calculated according to time intervals under the condition that the size of the cross section of the liquid channel is known; when the sweat flow/flow rate is detected, the electrochemical detection unit in the cooperative detection device can collect concentration signals of components in the sweat in the liquid channel in real time, and the concentration data of the corresponding components in the sweat along with time change is calculated according to the signals, so that the coupling relation between the sweat flow/flow rate and the concentration change of various components in the sweat is established.
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