CN108414034A - A kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect - Google Patents
A kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect Download PDFInfo
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- CN108414034A CN108414034A CN201810111337.8A CN201810111337A CN108414034A CN 108414034 A CN108414034 A CN 108414034A CN 201810111337 A CN201810111337 A CN 201810111337A CN 108414034 A CN108414034 A CN 108414034A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F1/00—Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow
- G01F1/56—Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by using electric or magnetic effects
- G01F1/64—Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by using electric or magnetic effects by measuring electrical currents passing through the fluid flow; measuring electrical potential generated by the fluid flow, e.g. by electrochemical, contact or friction effects
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
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Abstract
A kind of Micropump that can monitor sweat flow in real time based on capillary evaporation effect, belongs to micro-fluidic chip system field.The Micropump realizes that the collection and transmission of sweat, the integrated electrode in channel can monitor sweat flow in real time by capillary evaporation effect.The Micropump is divided into three layers, and only there are three hexagon outlet opening, each outlet opening is made of top layer multiple apertures and the identical hole array of pitch-row.Middle layer is made of an entrance, three outlets and three trouble channels, and outlet port corresponds to the outlet opening of top layer respectively.Bottom integrates three electrodes comprising an entrance and in the center corresponding to middle layer main channel, is from left to right followed successively by the reference electrode made by gold, the working electrode made by platinum and by gold making to electrode.Three electrodes and computer system are connect with electrochemical workstation respectively in the present invention, are observed current value in computer system and are obtained the real-time traffic of microchannel, i.e. rate of perspiration using the limitation steady-state response equation of flow-thru electrode.
Description
Technical field
The invention belongs to micro-fluidic chip system fields, and in particular to and it is a kind of to collect sweat using capillary-evaporative effect, together
Shi Liyong electrochemical principles monitor the micro-fluidic chip of sweat flow in real time.
Background technology
Contain abundant physiology and metabolic information in human sweat, can be used for medical diagnosis on disease, drug abuse detection and movement
Performance optimization etc. can obtain the useful information about body physiological situation by analyzing the constituent of sweat.Certain heredity
Disease (such as cystic fibrosis) can be diagnosed from the sweat composition of baby.It is strong with the mankind alternatively, it is also possible to assess sweat
Health shows relevant several key organism markers, and such as rate of perspiration, it may determine that risk of the sportsman with the presence or absence of dehydration, compared with
High dehydration value can influence cardiovascular system, lead to heat cramps or heatstroke, be helped to the monitoring of the variation of rate of perspiration over time
In the fluid infusion strategy for formulating personalization.One of the main points must take into consideration when sweat analysis are the collection modes of sweat, to obtain
Obtain most reliable result.If without automation mechanized operation, these preparation process are time-consuming and laborious, and easy to produce artificial behaviour again
It slips up, to seriously affect analysis result.For many years, people use different sweat collection technologies, and such as " whole body rinses
Art ", the loss of whole body sweat depend on the weight before and after human motion, the lossy liquid of synchronous collection and storage institute；Using by
Patch or capsule made of flexible adhesives film are covered by air-locked lab film paper to prevent the sweat of sweat evaporation
Collection technique.However, these technologies cannot provide the real time information about physical condition, mainly due to sampling, processing and after
Test analysis in sample cross contamination risk it is very high and carry out be delay analysis.
In recent years, fast-developing microflow control technique is structure formed with its, required sample milligram ammonia, can accurately control stream
Body and have many advantages, such as good bio-compatibility for sweat monitoring with analysis provide possibility.Micro-fluidic chip utilizes at present
Gravity, surface tension, capillary force and osmosis etc. collect sweat, i.e., are realized by changing the potential energy of fluid in fluid channel
Fluid drives.Wherein, the flow-rate adjustment ability of gravity drive Micropump is limited by liquid level；It surface tension, capillary force and oozes
The Micropump of effect driving, flow are not sufficiently stable thoroughly, and discharge stability is difficult to keep when working long hours, when fluid system is filled out completely
When filling, driving force stops.The Micropump realized using the combination of capillary-evaporation not only driving force it is stronger and also can generate it is continuous and
Extended flow, in principle being capable of passively long-play.For lasting sweat sensing and monitoring, can directly wear
The compact wearing formula device for collecting and analyzing on the skin and continuously sweat would be desirable solution, not only automatic
Absorb sweat, but also include the automatic filling of sweat, along in device microchannel and induction cavity generate continuous stream
It is dynamic.
About the detection of rate of perspiration or sweat flow, current research concentrates on can be with the perspiration of local measurement body part
Rate method includes being detected dependent on the measurement impedance method of Skin Resistance and based on integrated humidity capacitance sensor on fabric
The device of sweat rate loses to quantify sweat, other common methods include being carried out in the front and back patch on subjects skin of movement
It weighs.Impedance method, which is vulnerable to the factor changed as caused by strong mood, scaring event, pressure, to be influenced；Humidity capacitance sensing
Device method is difficult to keep the fixed range between sensor and is easy to be influenced to lead to wrong report/negative by correction of motion artefacts；Paster method
Since tested time longest may block sweat gland pipeline or change the micro climate close to skin.Fast-developing Electrochemical Detection
Technology is with its electrode miniaturization, high sensitivity, required power are low, can in real time monitoring signals and it is easy of integration in a device the advantages that be
Carrying out flow in micro-fluid chip, detection and analysis provide possibility in real time.Ideal sweat monitoring device not only can the company of generation
Continuous and extended flow can also provide detailed real-time traffic information, therefore a kind of utilization capillary-evaporative effect of present invention design
The micro-fluidic chip system collected sweat and monitor its flow in real time is realized with electrochemical gauging amount method.
Invention content
In view of the problems of the existing technology, the present invention, which devises, a kind of not only may be implemented sweat collection and can also extend
To the flexible controllable evaporation transfer tube of real-time monitoring body different parts rate of perspiration variation, it is easier to than current measurement method
Using with it is efficient.It realizes the collection and transmission of sweat by capillary-evaporative effect, the electrode integrated in conjunction with microfluidic channel
Sweat flow in detection Micropump in real time.It allows the variation for dynamically over time tracking rate of perspiration, applicable without being to provide
In the single-measurement entirely tested.
Present invention combination microflow control technique collects and detects sweat with electrochemical measuring technique.The integrated electrode in microchannel with
Computer system is connect with electrochemical workstation respectively, and the current value in channel can be flowed through in computer system dynamic observation sweat,
And the flow for flowing through microchannel can be calculated using the limitation steady-state response equation of flow-thru electrode, you can obtain rate of perspiration.
To achieve the goals above, technical scheme is as follows：
A kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect, the Micropump include collecting sweat and examining
Survey the micro-fluidic chip (such as Fig. 1) and its peripheral system (such as Fig. 2) of sweat flow.The micro-fluidic chip is divided into three layers：Top
Layer, middle layer and bottom；Shown in overall structure such as Fig. 3 (a).
The top layer is that engraved structure is made of as shown in Fig. 3 (b) three Polygonal arrays, as shown in Fig. 3 (c),
Each Polygonal arrays includes multiple intensive evaporation apertures.
The middle layer is engraved structure, as shown in Fig. 3 (d), by a sweat collection entrance, three evaporation outlets
(being equivalent to liquid storage tank), main channel and three trouble channels are constituted；Sweat collection entrance passes sequentially through main channel and three trouble channels and steams
It sends out mouth to be connected, three evaporation outlet ports of middle layer correspond to the position of three Polygonal arrays of top layer respectively.
Include a sweat collection entrance and three-electrode system shown in the bottom such as Fig. 3 (e), three-electrode system and
The sensing chamber position of middle layer main channel is corresponding, is from left to right followed successively by the reference electrode made by golden (Au), by platinum (Pt)
The working electrode of making and by golden (Au) make to electrode；The length of electrode has to be larger than the width in channel, it is ensured that electrode with
Sweat in channel completely attaches to；Top layer is integrally manufactured with middle layer, is then bonded with bottom and (is used adhesive), in addition to sweat is received
Collect and is completely enclosed between three layers of micro-fluidic chip except entrance, main channel, three trouble channels and evaporation export.
The peripheral system includes skin area, calculator display organization and the electrochemical workstation for providing sweat.Three
Electrode system is connected with electrochemical workstation, constitutes a complete power circuit；Calculator display organization and electrochemical operation
It stands and is connected, for observing the corresponding current value of different flow.
Sweat is flowed into from sweat collection entrance, fills the sensing chamber where main channel and electrode through capillary action, then
The evaporation of polygon Process array generates powerful driving force, to drive fluid to generate continuous flowing, makes sweat successively
The sensing chamber where main channel, electrode and three trouble channels are flowed through, evaporation outlet is eventually arrived at.
The micro-fluidic chip overall dimensions are 40mm × 20mm × 0.75mm, and every layer is 250 μm soft by thickness
Property polymer material is made.
Each outlet opening includes 37 apertures in the top layer, and the diameter of each aperture is 250 μm, between fixed
Away from being 500 μm.
The sweat collection inlet diameter of the middle layer is 5mm；The diameter of three evaporation outlets is 4.1mm；It is main logical
Road size is 14.33mm × 0.75mm；Three trouble channels structure in, the symmetrical channel size in both sides be 3.45mm ×
0.25mm, intermediate channel size are 7mm × 0.25mm.
A diameter of 5mm of the bottom sweat collection entrance, three electrode sizes are 2mm × 1mm, are divided into 1mm.
In the middle layer, the place that sweat collection entrance, evaporation outlet, main channel and three trouble channels are connected is made
Arc-shaped prevents sweat to be bonded to corner to influence sweat flowing.
Computer system observation sweat flows through the current value in channel, and just using the limitation steady-state response equation of flow-thru electrode
The flow for flowing through microchannel can be calculated, you can obtain rate of perspiration.The evaporation rate theoretical calculation process of Micropump is as follows：
There are two theoretical parameter, the evaporation rates of total liquid volume altogether for MicropumpIt is driven with by the cross section of microchannel
Volume flow Q.The two must be balance.
Evaporation rate isBy theoretical calculation, and internal volume flow Q is determined by electrochemical measurements.In order to
Estimate evaporation rate, consider the evaporation by single hole first, as shown in Fig. 4 (a) and 4 (b), liquid is fixed on the edge in hole
And form the circular surface for having and limiting curvature by angle, θ.The fixed liquid that such case can be proposed by Picknett and Bexon
The evaporation model theory of drop describes, and gained equation is as follows：
Wherein, D is the diffusion coefficient of steam under environment temperature, and a is the radius of border circle, and M is the molecule matter of vapour molecule
Amount, ρ is the density of liquid.C(TW) and C (Ta) be respectively saturated water in liquid-gas interface and surrounding air mole vapor concentration.C
(TW) and C (Ta) sensitively depend on temperature.H is the relative humidity in environment.F (θ) is the function of contact angle θ shown in Fig. 4：
Equation (2) and (3) describe the evaporation rate of the single plane or curved surface that are defined by circle, wherein contact angle θ's
Ranging from θ=0 (the flat circular film i.e. in plane) to θ=π (circular drop i.e. in plane).The hypothesis of the theory is to steam
Hair is occurred by pure diffusion, and environment is still air, and temperature is uniform, and gravity does not work, and will not be occurred in drop
Liquid flows, and substrate is flat steady.For the sake of simplicity, contact angle is approximately θ=0 when model estimation evaporation rate.
Compared with isolated single hole, the hole evaporation of microwell array can be influenced by interacting between hole, and adjacent
It reduces in the steam partial place that adjacent pores gap generates.Therefore it is explained on surface between evaporation drop using evaporation correction factor (η)
Interaction.This factor depends on the geometry of droplet array, we use hexagonal array herein.In N
It, can be by evaporating correction factor (η by the evaporation rate in i-th of hole in the case of the array in a holei) it is multiplied by single hole
Evaporation rate describes：
Pass through i-th of hole (η in arrayi) evaporation correction factor follow following linear equation：
aiIt is the radius in i-th of hole, | ri-rj| it is the distance between i-th and jth hole.In the uniform pores that total hole count is N
In arrangement, averagely evaporating correction factor is
And evapo tranpiration rate is
And the principle that internal volume flow Q is measured by electrochemical method is as follows, measuring technique is surveyed based on electrochemistry flow
Determine method --- three-electrode electro Chemical system (electrochemical sensor) is embedded in the channel for filling fluid, and sensor is treated as
Flow sensor uses.When experiment, the sweat in channel can take more electroactive ions to electrode surface, lead to the increasing of electric current
Add.By the electrolyte transport of microfluidic channel, a timing amperometric signal can be obtained, signal and fluid flow are directly proportional
's.The three-electrode electro Chemical system integrated on Micropump is by reference electrode (Reference electrode, abbreviation RE), work electricity
Pole (Working electrode, abbreviation WE) and to electrode (Counter electrode, abbreviation CE) form.Three-electrode system
Containing two circuits, a circuit is made of working electrode and reference electrode, is used for the electrochemical reaction process of test job electrode,
Another circuit is made of working electrode and auxiliary electrode, is played transmission and is electronically formed circuit.Specific hydrodynamic condition
With high-quality transmission rate for the use of three-electrode electro Chemical system being successfully essential.According to energy, this special (Nernst) is approximate
The thickness of method, diffusion layer (δ) is related to liquid inventory (U) experience,
δ=B/Uα (8)
Wherein δ is the thickness of diffusion layer, and B is adsorption coefficient, and α is transfer ratio, and range is between 0.33 and 1.0.C is stream
Cross the solution concentration in channel, C, B and α are given constants.The general electric current of mass transfer control reaction is substituted into equation (8)
It responds in (9),
i1=(nFADC/ δ) (9)
Obtain the limitation steady-state response of flow-thru electrode:
K hereinmIt is mass transport coefficient (D/B), F is Faraday constant.
Consider the hydrodynamic characteristics of fluent solution.Therefore, by solving three-dimensional convection diffusion equation, needle now
Various electrode geometries have been derived with the expression formula of carrying current (under steady state conditions, a reactor).Wherein, table 1, which gives, passes through setting
The result equation that suitable initial and boundary condition (depending on special electrodes) obtains.
| Electrode geometry | Current limliting equation |
| Tubulose | I=1.61nFC (DA/r)2/3U1/3 |
| Plane (concurrent flow) | I=0.68nFCD2/3v-1/6(Ab)1/2U1/2 |
| Thin layer | I=1.47nFC (DA/b)2/3U1/3 |
| Plane (vertical) | I=0.903nFCD2/3v-1/6A3/4u1/2 |
| Wall Jet detector | I=0.898nFCD2/3v-5/12a-1/2A3/8U3/4 |
The corresponding carrying current expression formula of 1 different geometries electrode of table
In table, wherein a=sweat collections inlet diameter, A=electrode areas, b=channel heights, C=fluid concentrations
(mM), F=Faraday constants, D=diffusion coefficients, ν=kinematic viscosity, the radius of r=hollow edged electrodes, U=volume flows, u=
Speed (cm/s), n=electron numbers.
We detect the flow in Micropump channel using thin layer strip shaped electric poles, therefore required equation is
I=1.47nFC (DA/b)2/3U1/3 (11)
Beneficial effects of the present invention：Apparatus of the present invention design structure is simple, and control process is convenient, can be changed by heater
Flow.Therefore the Micropump, which removes, has the not external energy, and run time is unrestricted, can be arbitrarily for a long time (as long as the phase of environment
To humidity<100%) outside the advantages of running, the conventional machining techniques that low cost also can be used make, and can as needed design not
With the micropump of flow.A kind of Micropump of wearable detection rate of perspiration is designed, its manufacture should be cheap, uses flexibility as possible
Material is to ensure the good contact between system and skin and can predict pump rate.
Description of the drawings
Fig. 1 is the micro-fluidic chip collected sweat and monitor its flow in real time.
Fig. 2 is peripheral system.
Fig. 3 (a) is the overall structure figure of micro-fluidic chip.
Fig. 3 (b) is the top level structure figure of micro-fluidic chip.
Fig. 3 (c) is the evaporation outlet hole structure figure of hexagon microwell array.
Fig. 3 (d) is the interlayer structure figure of micro-fluidic chip.
Fig. 3 (e) is the fabric figure of micro-fluidic chip.
Fig. 4 (a) is the evaporation model schematic diagram of solid drop single aperture.
Fig. 4 (b) is the schematic diagram of Liquid contact angle θ in hole.
In figure：1 micro-fluidic chip；2 collect the skin area of sweat；3 calculator display organizations；4 electrochemical workstations；5
Three-electrode system；6 sweat collection entrances；7 evaporation outlets.
Specific implementation mode
Below in conjunction with specific implementation mode, the present invention will be further described.
A kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect, the Micropump include collecting sweat and examining
Survey the micro-fluidic chip 1 (such as Fig. 1) and its peripheral system (such as Fig. 2) of sweat flow.The micro-fluidic chip 1 includes three electrodes
System 5, sweat collection entrance 6 and the evaporation outlet 7 with hexagon microwell array structure, peripheral system include providing sweat
Skin area 2, calculator display organization 3 and electrochemical workstation 4 etc..Micropump is fixed on the sweat collected with stickup bandage
And the skin area 2 of rate of perspiration is detected, electrochemical workstation 4 is connected with three-electrode system 5 later, constitutes a complete energization
Circuit；Calculator display organization 3 is connected with electrochemical workstation 4, for observing the corresponding current value of different flow.
It is three layers that micro-fluidic chip 1, which divides,：Top layer, middle layer and bottom, shown in overall structure such as Fig. 3 (a)；The top layer
It is made of three Polygonal arrays as shown in Fig. 3 (b) for engraved structure, as shown in Fig. 3 (c), each Polygonal arrays is equal
Including multiple intensive evaporation apertures；The middle layer is engraved structure, as shown in Fig. 3 (d), by sweat collection entrance 6, three
7 (being equivalent to liquid storage tank) of evaporation outlet, main channel and three trouble channels are constituted；Sweat collection entrance 6 passes sequentially through main channel and three
Trouble channel is connected with evaporation outlet, and three evaporation outlets, 7 positions of middle layer correspond to three Polygonal arrays of top layer respectively.Bottom
Layer is as shown in Fig. 3 (e), including a sweat collection entrance 6 and three-electrode system 5, three-electrode system 5 and middle layer main channel
Sensing chamber position is corresponding, is from left to right followed successively by the reference electrode made by golden (Au), the working electrode made by platinum (Pt)
With by golden (Au) make to electrode；The length of electrode has to be larger than the width in channel, so that electrode and the sweat in channel are complete
Full connected；Top layer is integrally manufactured with middle layer, is then bonded with bottom and (is used adhesive), in addition to sweat collection entrance 6, master are logical
It is completely enclosed between three layers of micro-fluidic chip except road, three trouble channels and evaporation outlet 7.
Sweat is flowed into from sweat collection entrance 6, fills the sensing chamber where main channel and electrode through capillary action, then
The evaporation of polygon Process array generates powerful driving force, to drive fluid to generate continuous flowing, makes sweat successively
The sensing chamber where main channel, electrode and three trouble channels are flowed through, evaporation outlet 7 is eventually arrived at.
1 overall dimensions of micro-fluidic chip are 40mm × 20mm × 0.75mm, and every layer is 250 μm poly- by thickness
Methyl methacrylate (Polymethylmethacrylate, abbreviation PMMA) is made, this be in order to ensure system and skin it
Between good contact and human body comfort level.Each outlet opening includes 37 apertures in the top layer, each aperture
Diameter is 250 μm, and constant spacing is 500 μm.The middle layer sweat inlet diameter is 5mm, and three sweat export straight
Diameter is 4.1mm, and main channel size is 14.33mm × 0.75mm；In the structure in three trouble channels, the symmetrical channel size in both sides
It is 3.45mm × 0.25mm, intermediate channel size is 7mm × 0.25mm.In the intermediate layer, sweat entrance, sweat outlet, master
Arc-shaped is made in the place that channel and three trouble channels are connected, and prevents sweat to be bonded to corner to influence sweat flowing.Institute
The a diameter of 5mm for the bottom sweat collection entrance 6 stated, three-electrode system 5 is corresponding with the centre position of middle layer main channel, and three
A electrode size is 2mm × 1mm, is divided into 1mm.The length of electrode has to be larger than the width in channel, so as to electrode and channel
In sweat completely attach to, ensure measure accuracy.
The selected personnel to be measured being going strong participate in the indoor circulation sweat experiment of approved programme.They are in holding temperature
25 DEG C and stuffy indoor circulation 15-20 minutes of relative humidity of 40%.(self can be carried out with the movement of enough high intensity
Selection) it can for example be perspired by recycling dynamometer or riding cycle ergometer to induce, fixed position is physically selected to receive
Collect sweat.Micropump is located in upper arm by pasting bandage, selects external forearm as main sampling location, it provides a phase
Collection device is fixed to flat surface.When volunteer starts the cycle over, electrochemical workstation is started to work.Each test bag
Include three phases.First stage：Fundamentals of Measurement rate of perspiration.Second stage：Personnel to be measured reach failure peak.It is recorded in this stage
The maximum value of rate of perspiration.Phase III：When recovery, rate of perspiration is restored to a reference value.Tester is big before second stage terminates
Stop high-intensity exercise within about 1 minute.On average, everyone recycles about 15 minutes, most 20 minutes every time.When tester cannot
It maintains to stop experiment when setting intensity load.
During experiment, the position of Simultaneous Monitoring Micropump keeps optimum contact with the skin area for ensuring and sampling.Micropump
Entrance and skin are in close contact, this be in order to prevent sweat in the additional evaporation of inlet.When sweat is flowed into from the entrance of Micropump
Then the sensing chamber where microchannel and electrode is filled through capillary action；Filter paper is added in microchannel can prevent in this process
It is middle to form any liquid interlayer or air inclusion, because interlayer can influence the flowing of sweat.Polygon Process array
Evaporation generates powerful drive force sweat and generates continuous flowing, and sweat is made to flow successively through main channel, electrode and three trouble channels,
Eventually arrive at evaporation outlet.Once thering is the sweat continuously flowed to flow through electrode, the electrochemical workstation being connected with three-electrode system
Enter working condition immediately, electric current starts to generate at this time, as the ending current of experiment slowly stops and is restored to a reference value.
The data of electric current can be shown and acquired by the computer system being connected with electrochemical workstation, pass through flow-thru electrode
Limitation steady-state response equation, that is, formula (11) flow for flowing through microchannel can be calculated.Test of many times and the electricity for choosing acquisition
After flow data data draw out electric current-flux scale directrix, it can directly prejudge outflow value by current values, i.e. human body goes out
Sweat rate.
Claims (10)
1. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect, which is characterized in that the Micropump includes collecting
Sweat and the micro-fluidic chip and its peripheral system for detecting sweat flow；The micro-fluidic chip is divided into three layers：Top layer, centre
Layer and bottom；
The top layer is engraved structure, is made of three Polygonal arrays, each Polygonal arrays includes multiple intensive
Evaporate aperture；
The middle layer is engraved structure, by a sweat collection entrance (6), three evaporation outlets (7), main channel and three troubles
Channel is constituted；Sweat collection entrance (6) passes sequentially through main channel and three trouble channels and is connected with evaporation outlet (7), three, middle layer
The position of evaporation outlet (7) corresponds to the position of three Polygonal arrays of top layer respectively；
The bottom includes a sweat collection entrance (6) and three-electrode system (5), three-electrode system (5) and middle layer master
The sensing chamber position in channel is corresponding, be from left to right followed successively by the reference electrode made by Au, by the Pt working electrodes made and
By Au making to electrode；The length of electrode has to be larger than the width in channel, it is ensured that electrode is completely attached to the sweat in channel；
Top layer is integrally manufactured with middle layer, is then bonded with bottom, in addition to sweat collection entrance (6), main channel, three trouble channels and steaming
It sends out and is completely enclosed between three layers of micro-fluidic chip except mouth (7)；
The peripheral system includes skin area (2), calculator display organization (3) and the electrochemical workstation for providing sweat
(4)；Three-electrode system (5) is connected with electrochemical workstation (4), constitutes a complete power circuit；Calculator display organization
(3) it is connected with electrochemical workstation (4), for observing the corresponding current value of different flow；
Sweat is flowed into from sweat collection entrance (6), fills the sensing chamber where main channel and electrode through capillary action, then more
The evaporation of side shape Process array generates powerful driving force, to drive fluid to generate continuous flowing, sweat is made to flow successively
The sensing chamber where main channel, electrode and three trouble channels are crossed, evaporation outlet (7) is eventually arrived at.
2. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 1, feature
It is, the micro-fluidic chip (1) overall dimensions are 40mm × 20mm × 0.75mm, and every layer is 250 μm soft by thickness
Property polymer material is made.
3. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 1 or 2,
It is characterized in that, each outlet opening includes 37 apertures in the top layer, and the diameter of each aperture is 250 μm, fixed
Spacing is 500 μm.
4. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 1 or 2,
It is characterized in that, sweat collection entrance (6) a diameter of 5mm of the middle layer；Three evaporation outlet (7) diameters be
4.1mm；Main channel size is 14.33mm × 0.75mm；In the structure in three trouble channels, the symmetrical channel size in both sides is
3.45mm × 0.25mm, intermediate channel size are 7mm × 0.25mm.
5. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 3, feature
It is, sweat collection entrance (6) a diameter of 5mm of the middle layer；The diameter of three evaporation outlets (7) is 4.1mm；It is main
Channel size is 14.33mm × 0.75mm；Three trouble channels structure in, the symmetrical channel size in both sides be 3.45mm ×
0.25mm, intermediate channel size are 7mm × 0.25mm.
6. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 1,2 or 5,
It is characterized in that, a diameter of 5mm of the bottom sweat collection entrance (6), three electrode sizes are 2mm × 1mm, interval
For 1mm.
7. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 3, feature
It is, a diameter of 5mm of the bottom sweat collection entrance (6), three electrode sizes are 2mm × 1mm, are divided into 1mm.
8. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 4, feature
It is, a diameter of 5mm of the bottom sweat collection entrance (6), three electrode sizes are 2mm × 1mm, are divided into 1mm.
9. a kind of according to claim 1,2,5,7 or 8 can monitor the micro- of sweat flow in real time based on capillary-evaporative effect
Pump, which is characterized in that in the middle layer, sweat collection entrance (6), evaporation outlet (7), main channel and three trouble channels connect
Arc-shaped is made in the place connect, prevents sweat to be bonded to corner to influence sweat flowing.
10. a kind of Micropump that can monitor sweat flow in real time based on capillary-evaporative effect according to claim 6, special
Sign is, in the middle layer, sweat collection entrance (6) evaporates the ground that outlet (7), main channel and three trouble channels are connected
Arc-shaped is made by side, prevents sweat to be bonded to corner to influence sweat flowing.
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