CN206057151U - Numeration of leukocyte device based on mobile terminal - Google Patents
Numeration of leukocyte device based on mobile terminal Download PDFInfo
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- CN206057151U CN206057151U CN201621120010.XU CN201621120010U CN206057151U CN 206057151 U CN206057151 U CN 206057151U CN 201621120010 U CN201621120010 U CN 201621120010U CN 206057151 U CN206057151 U CN 206057151U
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Abstract
This utility model provides a kind of numeration of leukocyte device based on mobile terminal, including mobile terminal, electrochemical workstation and sensor, described sensor signal is received by the Arduino systems in electrochemical workstation and is sent to mobile terminal by bluetooth module, and described sensor includes electrode body, encapsulated layer and pedestal；By the numeration of leukocyte device based on mobile terminal, we rapidly using 10 microlitres of blood sample in the concentration of a big dynamic range quantification leukocyte, and can reach the error less than 10%.
Description
Technical field
This utility model belongs to numeration of leukocyte field, more particularly to a kind of numeration of leukocyte based on mobile terminal is filled
Put.
Background technology
Leukocyte analysis provide what is enriched for the management of acute bacterial and the quick diagnosis and chronic disease of virus infection
The patient of information, particularly immunodeficiency or the number of white blood cells of leukemia patient persistently will be monitored.
Traditional numeration of leukocyte is counted using blood counting instrument under microscope, and the actual application value of this method is non-
It is often limited, to waste time and energy, flux is low.The numeration of leukocyte of the full-automatic efficient clinically applied is by optical scattering, glimmering
Signal or leukocyte resistance principle are realizing.This full-automatic blood counting instrument needs large-scale equipment and trained
Operator, is very suitable for specialized laboratory's application of various medical institutions, but is not suitable for quick diagnosis, be also unsuitable for money
The limited developing country in source.Therefore small-sized portable blood counting instrument has wide application demand.Research and develop at present
Some small white blood cells that leukocyte is separated and collected using microstream are gone out and have counted platform, because remaining a need for making using microscope
For detecting system, these platforms still suffer from problem in terms of portability.Numeration of leukocyte microstream electricity based on Coulter principles
Impedance induction apparatuss have a channel blocking, and then have impact on the performance of system, the microstream system being previously mentioned according to
Rely in the outside main equipment such as liquid pump and signal acquisition so which is can be only applied in the laboratory table of laboratory.
It is time-consuming based on current leukocyte analysis technology, consume thing, work consuming depends on large-scale instrument and trained
Member.Quickly, inexpensive and portable leukocyte analysis instrument is urgently researched and developed.
Utility model content
For the deficiencies in the prior art, the present invention is a kind of to provide a kind of without the need for token, the microporous membrane based on mobile terminal
Electrochemistry numeration of leukocyte device, by using this technique device, we can rapidly apply 10 microlitres of blood sample,
The concentration of one big dynamic range quantification leukocyte, and the error less than 10% can be reached.
To realize goal of the invention, this utility model takes following technological means：
Based on the numeration of leukocyte device of mobile terminal, including mobile terminal, electrochemical workstation and sensor, it is described
Sensor signal is received by the Arduino systems in electrochemical workstation and is sent to mobile terminal by bluetooth module, described
Sensor include electrode body, encapsulated layer and pedestal；The electrode body includes perforated membrane or metal film, in perforated membrane or gold
Titanium is plated successively using metal vapor deposition process on category film and gold forms titanium layer and layer gold, by cut on acrylic resin
Interdigital electrode is formed into the electrode pattern of photoresist, the interdigital electrode exit is connected with three leadout electrodes；Described
Electrode body is placed in encapsulated layer, and encapsulated layer is being arranged on pedestal.
Preferably, difference arteries and veins potential difference DPV measuring systems are provided with described mobile terminal.
Preferably, the one kind of described described perforated membrane in pvdf membrane, nitrocellulose membrane or glass fibre membrane；
Described metal film is selected from golden film, silverskin or copper film.
Preferably, the width of described interdigital electrode is 500 μm, the thickness of described titanium layer is 10nm, described gold
The thickness of layer is 250nm.
Preferably, the material of three described leadout electrodes is spun gold or copper cash.
Preferably, three described leadout electrodes connect respectively in electrochemical workstation to electrode, reference electrode and
Working electrode.
Preferably, the encapsulated layer includes that lower floor, embracing layer and upper strata, embracing layer and upper strata are the solid of hollow out
Shape, lower floor are chimeric with the openwork part of embracing layer, and upper strata is provided with the groove that can place three leadout electrodes.
Preferably, the material of described encapsulated layer is polydimethylsiloxane colloid.
The Cleaning Principle with regard to the micropore membrane electrochemical numeration of leukocyte device based on mobile terminal of the present invention is we
Method Applied Electrochemistry principle on the flexible electronics of pvdf membrane is detection platform based on mobile terminal, and cell and molecule are entered
Row quantitative analyses, process are that biological specimen (cell or molecule) is quantitatively adding sensor surface, and electrochemical workstation is to passing
Sensor electrode making alive, and curent change is detected, mobile terminal is passed to data by bluetooth, the concentration of leukocyte is depended on
Gaussian peak (base in the current peak (in the case that baseline is constant) produced in DPV measuring systems or current-voltage relation
The situation that line changes), the peak point current for measuring is by Bluetooth transmission to mobile terminal.
By the micropore membrane electrochemical numeration of leukocyte device based on mobile terminal for applying the present invention, we can be rapid
10 microlitres of application blood sample, in the concentration of a big dynamic range quantification leukocyte, and can reach and be less than
10% error, and the device quickly cell can be carried out it is quantitatively quick so as to reach in the case of resource-constrained, it is low
Cost carries out leukocyte analysis, and having can be used for POCT application, white blood cell detection model due to wireless blue tooth communication characteristic
Enclose up to (102～104μL-1), can be effectively applied to include that the POCT of leukocytosiss and leukopenia etc. is examined
It is disconnected.
Description of the drawings
Fig. 1 is the structural representation of the micropore membrane electrochemical numeration of leukocyte device based on mobile terminal；
1. mobile terminal；2. electrochemical workstation；3. sensor；4. sample；
Fig. 2 is the internal anatomy of sensor in Fig. 1；
Fig. 3 is the schematic diagram of the connection of electrochemical workstation and sensor in Fig. 1.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.Following examples will be helpful to this area
Technical staff further understand the present invention, but the invention is not limited in any way.It should be pointed out that to the general of this area
For logical technical staff, without departing from the inventive concept of the premise, some deformations and improvement can also be made.These belong to
Protection scope of the present invention.
Embodiment
The micropore membrane electrochemical numeration of leukocyte device based on mobile terminal as shown in Figure 1 to Figure 3, including mobile terminal
1st, electrochemical workstation 2 and sensor 3, described sensor signal are received simultaneously by the Arduino systems in electrochemical workstation
Mobile terminal is sent to by bluetooth module, described sensor includes electrode body 21, encapsulated layer and pedestal 22；The electrode
Body includes pvdf membrane, plates titanium successively using metal vapor deposition process and gold forms titanium layer and layer gold, by swashing on pvdf membrane
Light cutting forms interdigital electrode on acrylic resin into the electrode pattern of photoresist, and the interdigital electrode exit is connected with
Three leadout electrodes 23；Described electrode body 21 is placed in encapsulated layer, and encapsulated layer is being arranged on pedestal 22；Described movement
Difference arteries and veins potential difference DPV measuring systems are installed in terminal；The width of described interdigital electrode is 500 μm, described titanium layer
Thickness is 10nm, and the thickness of described layer gold is 250nm；The material of three described leadout electrodes 23 is spun gold；Described three
Root leadout electrode connect respectively in electrochemical workstation to electrode, reference electrode and working electrode；Under the encapsulated layer includes
Layer 221, embracing layer 222 and upper strata 223, embracing layer and upper strata are the openwork part of the solid figure of hollow out, lower floor and embracing layer
Chimeric, upper strata is provided with the groove that can place three leadout electrodes；The material of described encapsulated layer is polydimethylsiloxane
Colloid.
Pvdf membrane in embodiment is perforated membrane, can also be replaced with nitrocellulose membrane or glass fibre membrane；Another pvdf membrane
To be replaced with metal film, metal film may be selected from golden film, silverskin or copper film.
The Cleaning Principle with regard to the micropore membrane electrochemical numeration of leukocyte device based on mobile terminal of the present invention is we
Method Applied Electrochemistry principle on the flexible electronics of pvdf membrane is detection platform based on mobile terminal, and cell and molecule are entered
Row quantitative analyses, process are that biological specimen (cell or molecule) is quantitatively adding sensor surface, and electrochemical workstation is to passing
Sensor electrode making alive, and curent change is detected, mobile terminal is passed to data by bluetooth, the concentration of leukocyte is depended on
Gaussian peak (base in the current peak (in the case that baseline is constant) produced in DPV measuring systems or current-voltage relation
The situation that line changes), the peak point current for measuring is by Bluetooth transmission to mobile terminal.
Here the biological specimen for adding can be human leukocyte or other biological cell classes, the such as cell line of people or thin
Bacterium, by the device, does not enter and leukocyte can be counted, and works together for other biological cells can also reach counting mesh
's.
Embodiment described above is only that preferred implementation of the present utility model is described, not to this practicality
New spirit and scope are defined, on the premise of without departing from this utility model design concept, ordinary skill in this area
Various modifications and improvement that technical staff is made to the technical solution of the utility model, all should fall into protection model of the present utility model
Enclose, the technology contents that this utility model is claimed, all recorded in detail in the claims.
Claims (8)
1. the numeration of leukocyte device based on mobile terminal, it is characterised in that including mobile terminal, electrochemical workstation and sensing
Device, described sensor signal are received by the Arduino systems in electrochemical workstation and are sent to movement by bluetooth module
Terminal, described sensor include electrode body, encapsulated layer and pedestal；The electrode body includes perforated membrane or metal film,
Titanium is plated successively using metal vapor deposition process on perforated membrane or metal film and gold forms titanium layer and layer gold, by cut third
Interdigital electrode is formed into the electrode pattern of photoresist on olefin(e) acid resin, the interdigital electrode exit is connected with three and derives electricity
Pole；Described electrode body is placed in encapsulated layer, and encapsulated layer is being arranged on pedestal.
2. the numeration of leukocyte device based on mobile terminal according to claim 1, it is characterised in that described mobile end
Difference arteries and veins potential difference DPV measuring systems are installed in end.
3. the numeration of leukocyte device based on mobile terminal according to claim 1, it is characterised in that described perforated membrane
One kind in pvdf membrane, nitrocellulose membrane or glass fibre membrane；Described metal film is selected from golden film, silverskin or copper film.
4. the numeration of leukocyte device based on mobile terminal according to claim 1, it is characterised in that described interdigital electricity
The width of pole is 500 μm, and the thickness of described titanium layer is 10nm, and the thickness of described layer gold is 250nm.
5. the numeration of leukocyte device based on mobile terminal according to claim 1, it is characterised in that described three lead
Go out electrode connect respectively in electrochemical workstation to electrode, reference electrode and working electrode.
6. the numeration of leukocyte device based on mobile terminal according to claim 5, it is characterised in that described three lead
The material for going out electrode is spun gold or copper cash.
7. the numeration of leukocyte device based on mobile terminal according to claim 1, it is characterised in that the encapsulated layer bag
Include lower floor, embracing layer and upper strata, embracing layer and upper strata are the solid figure of hollow out, lower floor is chimeric with the openwork part of embracing layer,
Upper strata is provided with the groove that can place three leadout electrodes.
8. the numeration of leukocyte device based on mobile terminal according to claim 7, it is characterised in that described encapsulated layer
Material be polydimethylsiloxane colloid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621120010.XU CN206057151U (en) | 2016-10-13 | 2016-10-13 | Numeration of leukocyte device based on mobile terminal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621120010.XU CN206057151U (en) | 2016-10-13 | 2016-10-13 | Numeration of leukocyte device based on mobile terminal |
Publications (1)
| Publication Number | Publication Date |
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| CN206057151U true CN206057151U (en) | 2017-03-29 |
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| CN201621120010.XU Active CN206057151U (en) | 2016-10-13 | 2016-10-13 | Numeration of leukocyte device based on mobile terminal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111781267A (en) * | 2020-05-06 | 2020-10-16 | 北京大学 | Arduino-based excitation signal generation circuit for realizing time current method and cyclic voltammetry and electrochemical detection device |
-
2016
- 2016-10-13 CN CN201621120010.XU patent/CN206057151U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111781267A (en) * | 2020-05-06 | 2020-10-16 | 北京大学 | Arduino-based excitation signal generation circuit for realizing time current method and cyclic voltammetry and electrochemical detection device |
| CN111781267B (en) * | 2020-05-06 | 2021-11-09 | 北京大学 | Arduino-based excitation signal generation circuit for realizing time current method and cyclic voltammetry and electrochemical detection device |
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