CN206489050U - A kind of impedance pulse grain count device based on inhomogeneous field - Google Patents
A kind of impedance pulse grain count device based on inhomogeneous field Download PDFInfo
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- CN206489050U CN206489050U CN201720179422.9U CN201720179422U CN206489050U CN 206489050 U CN206489050 U CN 206489050U CN 201720179422 U CN201720179422 U CN 201720179422U CN 206489050 U CN206489050 U CN 206489050U
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Abstract
The utility model discloses a kind of impedance pulse grain count device based on inhomogeneous field.The present apparatus includes glass negative, PDMS micro-fluidic chips, signal amplification component and signal acquisition control system, and the PDMS micro-fluidic chips fovea superior is carved with microchannel, and the microchannel includes：Two ends are respectively equipped with the main channel of oil-feed liquid storage hole and fuel-displaced liquid storage hole；From the main channel centre position, the sample intake passage extended to the direction away from the main channel, the sample intake passage end is provided with sample intake passage liquid storage hole；From the position apart from the main channel and sample intake passage intersection certain distance, the sense channel extended to the direction away from the main channel, the width of the sense channel and width ratio of main channel is fixed and end is provided with sense channel liquid storage hole.The utility model is simple in construction, and accuracy of detection is high, is that detection can be achieved with counting without particle turnover detection zone.
Description
Technical field
The utility model is related to particle monitoring technique field, particularly relates to a kind of impedance arteries and veins based on inhomogeneous field
Rush grain count device.
Background technology
Particle in sample is counted exactly and had great importance and demand in multiple fields；For example, in biology
Medical research, public health detection and the field such as marine environmental monitoring, research is used to determining object (such as bacterium, virus and
Marine microorganism etc.) number portable particle fast counting apparatus or method, have urgent demand always.
The method of current existing conventional grain count includes following several：
1) light blockage method (principle of optical interference)：Light blockage method is the grain count method being most widely used at present, also known as light
Obstruction method or light block method, are the sides that intensity variation occurred carries out Particle Sizing of blocking using particulate to light
Method.The method principle is simple, but needs costly and baroque photo-translating system, and accuracy of detection is low；
2) inductance method：Inductance method can change the phenomenon of micropore inductance using particle by detecting during micropore, to detection micropore
The change of inductance signal is monitored, it is possible to achieve grain count.The method principle is simple, and various metallic particles can be carried out
Detection, but metallic particles is only applicable to, accuracy of detection is also limited；
3) capacitance method：Capacitance method is a kind of non-contact detection method, when particle is by detection micropore, can change micropore
Electric capacity, by the change for monitoring micropore capacitance signal, it is possible to achieve grain count.The method can be used for low conducting solution
Grain count in (such as fluid), and capacitance detecting object is mainly metallic particles, but the detection of small capacitance depends on height
The detecting instrument of precision, thus limit the development of its portability.
4) laser-Induced Fluorescence Detection method：Particle carries fluorescence after laser irradiation, can be detected by photoelectric sensor
And realize grain count.The method is particularly suitable for use in blood analysis, immunology and microbiology association area, counts accurate, inspection
Survey convenient, but sample must be handled before detection, detection optical element cost used is high and complicated, it is impossible to detect
Atomic small particle；
5) resistance impulse method (RPS)：Resistance impulse method is based on when particle flows through two ends at the micropore that there is electric field, micropore
Voltage change and produce detection signal, realize detection；The method is easy to operate, is current precision highest grain count method.
Micro flow control chip device can be by biology, chemistry, the sample preparation of medical analysis process, reaction, separation and detection
On the chip that one piece of micro-meter scale is integrated into Deng basic operation, analysis overall process is automatically performed.It has liquid flowing it is controllable, disappear
Consumption reagent and sample are few, the features such as improve to analyze speed tenfold hundreds of times, can be in even shorter time a few minutes
Analyzed while up to a hundred samples of interior progress, and pretreatment and the analysis overall process of energy canbe used on line sample.
With the fast development of micro-fluidic chip process technology in recent years, existing many is utilized on micro-fluidic chip at present
The research report that RPS methods are counted to micro-nano particle, cell, bacterium and virus etc..Such as, typical micro-fluidic RPS counts core
Piece is that one section of line width sense channel more slightly larger than particle to be detected is processed in the middle part of the wide main channel of a hundreds of microns, then
Apply a DC electric field at main channel two ends；When the insulated particle in solution passes through sense channel its two ends component voltage can become
Change, obtain a pulse signal by using suitable signal acquiring system, the number of pulse signal is the number of particle.For
The convenient change for obtaining the polygonal voltage of sense channel two, people have invented different chip structures.
Initial detection chip structure only includes the wide main channel of a hundreds of microns and be arranged in the middle part of main channel one
The section line width sense channel more slightly larger than candidate particles, by will be inserted in after the platinum electrode series resistance at main channel two ends and direct current
Source two ends are connected, and grain count is realized in the change by amplifying resistance both end voltage signal, but the method system noise is big,
Signal to noise ratio is low, so accuracy of detection is low；Later detection chip respectively set up a detection arm passage before and after sense channel, use
Electrode direct measurement passage both end voltage signal, then by differential amplification, it is improved detection signal-to-noise ratio, and then cause detection
Precision is raised；Newest counting chip employs the structure of public inlet opening multichannel annular spread, and every passage all includes master
Power cathode is connected after passage, sense channel and liquid storage hole etc., its inlet opening connection positive source, each liquid storage hole series resistance,
Each passage is mutually as reference, and current counting chip can preferably eliminate system noise, by controlling each passage work successively
Make, realize that high flux is counted.It should be understood that, current counting chip is past in order to obtain higher detection signal-to-noise ratio
Toward it is required that the adjoining dimensions of sense channel size and particle, then correspond to the detection of nano particle it is also corresponding the need for process
The sense channel of nano-scale, but this is accomplished by extremely complex procedure of processing and expensive process equipment.
Further, since the size and particle of sense channel are closer to, particle occlusion detection passage is also easily caused；Together
When, the part that the impurity in solution will also result in sense channel is even completely plugged so that grain diameter testing result is missed
Difference or detection are interrupted, and have impact on precision and the stable operation of system.
Utility model content
In view of the deficiency that prior art is present, the purpose of this utility model is to provide for a kind of resistance based on inhomogeneous field
Anti- pulse grain count device, grain count is carried out using the present apparatus, and candidate particles enter main channel, Ran Houliu from sample channel
Do not enter sense channel through main channel and sample channel intersection, i.e. particle, so as to be prevented effectively from particle passage.
To achieve these goals, technical solutions of the utility model are as follows：
A kind of impedance pulse grain count device based on inhomogeneous field, it includes the micro-fluidic core of glass negative, PDMS
Piece, signal amplification component and signal acquisition control system, the PDMS micro-fluidic chips intaglio have side and the institute of microchannel
State glass negative to be packaged as a whole, to form the microchannel circulated for testing sample, it is characterised in that the microchannel includes：
Two ends are respectively equipped with the main channel of oil-feed liquid storage hole and fuel-displaced liquid storage hole；
From the main channel centre position, the sample intake passage extended to the direction away from the main channel, the sample intake passage
End is provided with sample intake passage liquid storage hole；
From the position apart from the main channel and sample intake passage intersection certain distance, to the remote main channel
The sense channel of direction extension, the width of the sense channel and width ratio of main channel is fixed and end is provided with sense channel
Liquid storage hole；
Inserted in platinum electrode, the sample intake passage hole in the sample intake passage liquid storage hole and sense channel liquid storage hole simultaneously
Platinum electrode connected by the positive pole of a reference resistance and dc source, the platinum electrode in the sense channel liquid storage hole with it is above-mentioned
The negative pole connection of dc source；The two ends of the reference resistance are connected by the input of wire and the signal amplification component
Connect, the output end of the signal amplification component is connected with the signal acquisition control system.
Further, as of the present utility model preferred：
The sense channel and main channel are angled, and the width ratio of itself and main channel is preferred to use 1/200.
Further, as of the present utility model preferred：
The signal amplification component uses difference amplifier element.
Further, as of the present utility model preferred：
The signal acquisition control system includes NI capture cards and computer.
Compared with prior art, the beneficial effects of the utility model：
1) the utility model is when being detected, its testing sample particle does not enter sense channel, so as to not result in logical
The phenomenon that road is blocked, detection stability is high；
2) the utility model improves constantly the signal to noise ratio of detection signal by increasing the ratio of main channel and sense channel,
So as to improve accuracy of detection.
Brief description of the drawings
Fig. 1 is microfluidic chip structure schematic diagram described in the utility model；
Fig. 2 is the structural representation of device described in the utility model.
In figure：M, micro-fluidic chip, L, glass negative, A, main channel oil-feed liquid storage hole, B, the fuel-displaced liquid storage hole in main channel, C,
Sample intake passage liquid storage hole, D, sense channel liquid storage hole, 1, main channel, 2, sample intake passage, 3, sense channel.
Embodiment
To make the purpose of this utility model, technical scheme and advantage clearer, implement below in conjunction with the utility model
Accompanying drawing in example, the technical solution of the utility model is clearly and completely described, it is clear that described embodiment is this
A part of embodiment of utility model, rather than whole embodiments.Based on the embodiment in the utility model, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the utility model protection
Scope.
When particle flows through the micropore that there is electric field, both end voltage at obvious disturbance, corresponding micropore can be produced to electric field
Change and produce voltage pulse signal.Detect the number of voltage pulse signal, you can realize the number of detection sample particle.
Based on above-mentioned design background, the utility model devises a kind of impedance pulse grain count based on inhomogeneous field
Device, below in conjunction with the accompanying drawings and specific embodiment further illustrates the technical solution of the utility model：
As shown in figure 1, a kind of impedance pulse grain count device based on inhomogeneous field, it include glass negative L,
PDMS micro-fluidic chips M, signal amplification component and signal acquisition control system.
The PDMS micro-fluidic chips intaglio has the side of microchannel and the glass negative to be packaged as a whole, to form confession
The microchannel of testing sample circulation, it is characterised in that：The microchannel includes：Two ends are respectively equipped with oil-feed liquid storage hole and fuel-displaced storage
The main channel of fluid apertures；From the main channel centre position, the sample intake passage extended to the direction away from the main channel, the sample introduction
Channel end is provided with sample intake passage liquid storage hole, and the width of the sample intake passage and the width ratio of main channel are fixed, and is preferably
1/10；From the position apart from the main channel and sample intake passage intersection certain distance, to the main channel into certain
The sense channel of the direction extension of angle, preferably the width ratio of the width of the sense channel and main channel is 1/200, and end
End is provided with sense channel liquid storage hole.
Inserted in the sample intake passage liquid storage hole and sense channel liquid storage hole in platinum electrode, the sample intake passage liquid storage hole
Platinum electrode connected by the positive pole of a reference resistance and dc source, the platinum electrode in the sense channel liquid storage hole with it is above-mentioned
The negative pole connection of dc source, to realize that testing sample flows into the electric osmose of main channel from sample intake passage liquid storage hole from sample intake passage
Transportation；Sample is flowed into behind main channel, and the fuel-displaced liquid storage hole in main channel is flowed under pressure differential；
The two ends of the reference resistance are connected by wire with the input of the signal amplification component.Preferably, institute
Signal amplification component is stated using difference amplifier element.
The output end of the signal amplification component is connected with the signal acquisition control system, to realize in sense channel
The amplification acquisition testing process of testing sample particle number signal.Preferably, the signal acquisition control system is gathered including NI
Card and computer.
Detected below by taking polystyrene sample particle as an example, as shown in Figure 2：
The device parameter of the implementation case：1*5 μm of the size (wide × high) of chip sense channel used in the implementation case,
Distance in the middle of each liquid storage hole to chip main channel is 5cm, and main channel size is 200*5 μm (wide × high), sample intake passage size
It is 20*5 μm (wide × high)；Detected sample is 3 μm of granules of polystyrene solution；Buffer solution is PBS (1 ×) solution；Put on
Sample intake passage liquid storage hole C and sense channel liquid storage hole D voltage is 48V；
The microchannel of the device includes main channel oil-feed liquid storage hole A, the main channel being located on the PDMS micro-fluidic chips
Fuel-displaced liquid storage hole B, sample intake passage liquid storage hole C, sense channel liquid storage hole D and main channel 1, sample intake passage 2 and sense channel 3；
Platinum electrode is inserted in sample intake passage liquid storage hole C and sense channel liquid storage hole D, and dc source two ends are connected to by resistance R；
In two inputs of the resistance R two ends by two difference amplifiers of conductor in parallel one；The output end of difference amplifier is connected to
The input of NI data collecting cards；NI output signals directly in the Computer display connected and can be analyzed.
The method that grain count is carried out based on above-mentioned grain count device, is comprised the following steps：
1) sample is added dropwise：A certain amount of PBS bufferings are added dropwise in sample intake passage liquid storage hole and sense channel liquid storage hole first
Liquid, is added dropwise a certain amount of dodecane in the fuel feed hole of main channel, it is ensured that oil liquid pressure is more than solution pressure always, then will be a certain amount of
Testing sample be added dropwise in sample channel inlet opening；
2) sample transport：The sample connected in dc source, sample intake passage liquid storage hole C is due to EOF and pressure effect quilt
It is transported in main channel, candidate particles in the fuel-displaced liquid storage hole B in main channel, detection process is then flowed under the pressure differential of main channel
Enter main channel from sample channel, then flow through main channel and sample channel intersection, i.e. particle and do not enter sense channel.Due to
Fluid is non-conductive, and fluid region is not in electric field in passage, therefore, by controlling the position of oil-water interfaces to limit
The distribution of electric field.In practice, it is necessary to ensure main channel in the distance with the oil-water interfaces of sense channel intersection to sense channel
Only 2-3 times of candidate particles particle diameter.
Due to only applying electric field, and the narrower in width of sense channel in sample intake passage and sense channel, therefore detection is logical
The electric-field intensity in road and main channel juncture area is very big (i.e. power line is very intensive).When particle is handed over by sense channel with main channel
During battery limit (BL) domain, disturbance electric field line can be easy to, sense channel component voltage is changed, the electricity at resistance R two ends is further resulted in
Buckling, produces detection signal, and detection signal is inputted to NI capture cards after the amplification of AD620 difference amplifiers differential signal,
NI capture cards output signal directly can show and be analyzed on the computer connected.
3) signal amplification collection analysis：Ginseng is gathered by the platinum electrode in sense channel liquid storage hole and sample intake passage liquid storage hole
The voltage pulse signal at resistance two ends is examined, after the signal collected is amplified by the difference amplifier being connected with reference resistance, by
Signal acquiring system is recorded and shows corresponding detection data, that is, detects the number of sample particle.
Testing result：The number that can directly obtain particle in real-time testing result, sample by computer is equal to pulse
The number of signal.
Using a kind of impedance pulse grain count device based on inhomogeneous field described in the utility model, detection process
In, particle solution is focused on using dodecane and flows through main channel and sense channel intersection, and the candidate particles are logical from sample
Road enters main channel, then flows through main channel and sample channel intersection.Due to sense channel and the electricity of main channel juncture area
Field intensity is very big, and particle does not enter sense channel, so as to effectively increase accuracy of detection and anti-channel block ability.
It is described above, only the utility model preferably embodiment, but protection domain of the present utility model is not
This is confined to, any one skilled in the art is in the technical scope that the utility model is disclosed, according to this practicality
New technical scheme and its utility model design are subject to equivalent substitution or change, should all cover in protection model of the present utility model
Within enclosing.
Claims (2)
1. a kind of impedance pulse grain count device based on inhomogeneous field, it include glass negative, PDMS micro-fluidic chips,
Signal amplification component and signal acquisition control system, the PDMS micro-fluidic chips intaglio have the side of microchannel and the glass
Glass egative film is packaged as a whole, to form the microchannel circulated for testing sample, it is characterised in that the microchannel includes：
Two ends are respectively equipped with the main channel of oil-feed liquid storage hole and fuel-displaced liquid storage hole；
From the main channel centre position, the sample intake passage extended to the direction away from the main channel, the width of the sample intake passage
The width ratio spent with main channel is fixed, and end is provided with sample intake passage liquid storage hole；
From the position apart from the main channel and sample intake passage intersection certain distance, to the direction away from the main channel
The sense channel of extension, the width of the sense channel and the width ratio of main channel are fixed, and end is provided with sense channel liquid storage
Hole；
Meanwhile, platinum electrode, the sample intake passage liquid storage hole are inserted with the sample intake passage liquid storage hole and sense channel liquid storage hole
Interior platinum electrode is connected by the positive pole of a reference resistance and dc source, the platinum electrode in the sense channel liquid storage hole with it is upper
State the negative pole connection of dc source；The two ends of the reference resistance are connected by the input of wire and the signal amplification component
Connect, the output end of the signal amplification component is connected with the signal acquisition control system.
2. grain count device according to claim 1, it is characterised in that：
The sense channel and main channel are angled, and the width ratio of itself and main channel is preferred to use 1/200.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720179422.9U CN206489050U (en) | 2017-02-27 | 2017-02-27 | A kind of impedance pulse grain count device based on inhomogeneous field |
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| CN201720179422.9U CN206489050U (en) | 2017-02-27 | 2017-02-27 | A kind of impedance pulse grain count device based on inhomogeneous field |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106644900A (en) * | 2017-02-27 | 2017-05-10 | 大连海事大学 | Pulse impedance particle counting device based on non-uniform electric field and particle counting method |
| CN109253963A (en) * | 2017-12-19 | 2019-01-22 | 北京纳米能源与系统研究所 | From driving grain count instrument and its method of counting |
-
2017
- 2017-02-27 CN CN201720179422.9U patent/CN206489050U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106644900A (en) * | 2017-02-27 | 2017-05-10 | 大连海事大学 | Pulse impedance particle counting device based on non-uniform electric field and particle counting method |
| CN106644900B (en) * | 2017-02-27 | 2023-10-03 | 大连海事大学 | Impedance pulse particle counting device based on non-uniform electric field and counting method thereof |
| CN109253963A (en) * | 2017-12-19 | 2019-01-22 | 北京纳米能源与系统研究所 | From driving grain count instrument and its method of counting |
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Granted publication date: 20170912 Effective date of abandoning: 20231003 |
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Granted publication date: 20170912 Effective date of abandoning: 20231003 |