CN108279202A - A kind of adjustable grain count device and method of accuracy of detection - Google Patents
A kind of adjustable grain count device and method of accuracy of detection Download PDFInfo
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- CN108279202A CN108279202A CN201810265887.5A CN201810265887A CN108279202A CN 108279202 A CN108279202 A CN 108279202A CN 201810265887 A CN201810265887 A CN 201810265887A CN 108279202 A CN108279202 A CN 108279202A
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- 238000001514 detection method Methods 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 69
- 238000003860 storage Methods 0.000 claims abstract description 42
- 239000002699 waste material Substances 0.000 claims abstract description 21
- 238000012545 processing Methods 0.000 claims abstract description 13
- 230000003321 amplification Effects 0.000 claims abstract description 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 12
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 11
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 11
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract description 11
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 11
- 239000002245 particle Substances 0.000 claims description 37
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 24
- 229910052697 platinum Inorganic materials 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 11
- 239000007853 buffer solution Substances 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 7
- 230000009471 action Effects 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
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- 238000012544 monitoring process Methods 0.000 description 4
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- 238000011160 research Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1023—Microstructural devices for non-optical measurement
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1024—Counting particles by non-optical means
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Abstract
The invention discloses a kind of adjustable grain count device and method of accuracy of detection, which includes that PDMS micro-fluidic chips, signal amplification circuit and Signal acquiring and processing unit, microchannel include：There are sample holes, the other end to have waste liquid hole for main channel, one end；Multiple first adjust channel, and described first adjusts channel one end, and there is the first liquid storage hole, the other end to intersect with the main channel and extend to the direction far from the main channel；And second adjust channel, one end is connected with detection mouth and perpendicular to the main channel and of same size with the main channel, and the detection mouth is located on the main channel apart from the main channel with the first adjusting channel intersection certain distance；The present invention can adjust accuracy of detection and can effectively avoid channel block as desired.
Description
Technical field
The present invention relates to particle monitoring technique fields, particularly relate to a kind of adjustable grain count device of accuracy of detection
And method.
Background technology
Grain count is widely used in the fields such as communications and transportation, machinery, electric power, oil and carries out solid particle to all kinds of fluid
Dustiness detects, while also in the fields such as biomedical, public health and environmental monitoring for determining pathogen, bacterium and cell
The number of equal objects, the research of portable quick particle counting device and method has great significance and demand.
The method of current existing common grain count includes following several：
1) light blockage method：The grain count method that is most widely used at present, it occurs blocking for light using particle
Intensity variation carries out Particle Sizing.The method principle is simple, but photo-translating system therein is constructively complex as well as expensive,
Accuracy of detection is not low also high.
2) inductance method：The phenomenon that causing micropore inductance to change when using particle by detecting micropore realizes grain count.This
Method And Principle is simple, but is only applicable to detect various metallic particles, metallic particles, and accuracy of detection is also limited by equipment precision.
3) capacitance method：A kind of non-contact detection method is believed by monitoring particle by the capacitance of micropore when detection micropore
Number variation realize grain count.The method is usually used in the grain count in low conducting solution (such as fluid), detects object master
To be metallic particles, the disadvantage is that the detection of small capacitance is dependent on expensive large-scale high-precision detecting instrument.
4) laser-Induced Fluorescence Detection method：Photoelectric sensor is real by detecting the fluorescence that particle carries after laser irradiation
Existing grain count.The method is suitable for blood analysis, immunology and microbiology related field, counts accurately and conveniently, disadvantage
It is that sample needs to pre-process, optical element structure used is complex and high cost, and accuracy of detection is limited.
5) resistance impulse method (RPS)：Inspection is realized in the variation of micropore both end voltage when monitoring particle flows through the micropore there are electric field
It surveys and counts, the method is easy to operate, is the highest grain count method of current precision.
It can be by basic operations collection such as biology, chemistry, the sample preparation of medical analysis process, reaction, separation and detections
At the micro flow control chip device on the chip to one piece of micro-meter scale, the flowing of exclusive liquid is controllable, consumes reagent and sample
Seldom, the features such as analyze speed is improved at ten hundreds of times so that in the time even shorter in a few minutes of a samples up to a hundred
It completes while analyzing, and pretreatment and the analysis overall process of canbe used on line sample.
The lab-on-chip technology rapidly developed in recent years, it was recently reported that many utilizes RPS methods pair on micro-fluidic chip
The research that micro-nano granules, cell and bacterium etc. are counted.Initial micro-fluidic RPS counting chips are waited in one section of line width ratio
The slightly larger sense channel both ends of the particle respectively wide main channel of one hundreds of microns of processing is detected, is then applied at main channel both ends straight
Galvanic electricity field；It can cause the variation of sense channel both ends component voltage when the insulated particle to be detected in solution passes through sense channel,
Output is pulse signal after this voltage change is amplified by circuit, and the number of the pulse signal occurred in detection process is to wait for
Survey the number of particle.Various optimizations have been carried out to chip structure to improve accuracy of detection, efficiency and operability, people.
Most traditional RPS detection chips structure only includes one section of line width sense channel more slightly larger than candidate particles and its both ends
The wide main channel of each hundreds of microns, by that will be inserted in after the platinum electrode series resistance at main channel both ends and DC power supply both ends
It is connected, grain count is realized in the variation for then amplifying resistance both end voltage signal.But the method system noise is big, so detection
Precision is low.Detection chip after optimization respectively sets up a detection arm channel again before and after sense channel, is directly measured with electrode
Channel both end voltage signal, then by differential amplification, system noise is reduced by difference, and then improve accuracy of detection；In recent years
A kind of counting chip based on inhomogeneous field occurred changes channel internal electric field using oil-water interfaces and is distributed, and utilizes flow field
Control realizes that candidate particles do not enter the detection under sense channel, solves particle disengaging sense channel and easily causes asking for blocking
Topic.
But existing counting chip higher detection signal-to-noise ratio in order to obtain, it generally requires so that sense channel size
With the adjoining dimensions of particle, procedure of processing is complicated and cost is higher, and is susceptible to the problem of sense channel blocks.And based on non-
The particle detection technique of uniform electric field, although particle does not have to enter sense channel, because of oil-water interfaces position in detection process
Set unstable, causing it to make, difficulty is big and stability is not high.In addition, limitation of the current detection chip because of testing principle, often
The accuracy of detection of a detection chip is fixed, and application range is limited.
Invention content
In view of defects in the prior art, the invention aims to provide a kind of adjustable grain count of accuracy of detection
Device, the device can adjust accuracy of detection and can effectively avoid channel block as desired.
To achieve the goals above, technical scheme of the present invention：
A kind of adjustable grain count device of accuracy of detection, which includes PDMS micro-fluidic chips, signal amplification circuit
And Signal acquiring and processing unit, the PDMS micro-fluidic chips are side and the load that microchannel is carved in PDMS substrate fovea superiors
Slide is packaged as a whole, to form the micro-fluidic chip for the microchannel circulated for sample to be tested, which is characterized in that the microchannel
Including：
There are sample holes, the other end to have waste liquid hole for main channel, one end；
Multiple first adjust channel, and described first, which adjusts channel one end, has the first liquid storage hole, the other end and the master
Intersect and extend to the direction far from the main channel in channel；
And second adjust channel, one end has the second liquid storage hole, and the other end is connected with detection mouth and perpendicular to institute
State main channel and of same size with the main channel, the detection mouth be located at apart from the main channel adjusted with described first it is logical
On the main channel of road intersection certain distance；
Wherein, it is inserted with platinum electrode in the sample holes, waste liquid hole, each first liquid storage hole and the second liquid storage hole；And
So that the platinum electrode in second liquid storage hole is connected by the cathode of a reference resistance and DC power supply, the sample holes are given up
Platinum electrode in fluid apertures, each first liquid storage hole is connect with the anode of above-mentioned DC power supply；The two of the reference resistance simultaneously
End is connect by conducting wire with the input terminal of the signal amplification circuit, the output end of the signal amplification circuit and the signal
Acquisition is connect with processing unit.
Further, as the preferred of the present invention：
The grain count device accuracy of detection is adjusted by changing the quantity that first adjusts channel.
Further, as the preferred of the present invention：
By changing grain count device accuracy of detection described in the length adjustment that second adjusts channel.
Another object of the present invention is to provide for a kind of method carrying out grain count based on above-mentioned grain count device, special
Sign is, includes the following steps：
S1, sample are added dropwise：PBS bufferings are added dropwise in the first liquid storage hole that sample holes and each first adjust channel first
Liquid is added dropwise PBS buffer solution in second liquid storage hole in the second adjusting channel and waste liquid hole, is then added dropwise to sample to be tested respectively
Into the sample holes；
S2, sample transport：DC power supply is connected, and adjusts liquid level in each liquid storage hole, makes to wait in the sample holes
Sample acts in pressure and flows into main channel, and sticking wall flow is after the adjustment in main channel under the differential pressure action for adjusting channel first
Waste liquid hole is flow to after the detection mouth in channel；
S3, signal amplify collection analysis：Acquire particle stream after testing mouth when reference resistance both ends voltage pulse signal, and
After collected signal is amplified, is recorded by the Signal acquiring and processing unit and show that corresponding detection data is examined
The number of sample particle.
Compared with prior art, beneficial effects of the present invention：
1) when being detected, sample to be tested particle does not enter detection mouth to the present invention, can effectively avoid channel blockage existing
As making it detect stability high；
2) present invention can constantly be carried by increasing the ratio of main channel and detection mouth width or by reducing channel height
The signal-to-noise ratio of high detection signal, and then improve accuracy of detection；
3) present invention can be adjusted by increasing the quantity of applied voltage, increased first adjusting channel or reducing second
Passage length, to improve accuracy of detection.
Description of the drawings
Fig. 1 is the microfluidic chip structure schematic diagram of device of the present invention；
Fig. 2 is the overall structure diagram of device of the present invention.
In figure：M, PDMS substrates, L, glass slide, A, first the first liquid storage hole, B, second the first liquid storage hole, C, third
A first liquid storage hole, D, main waste liquid hole, E, the second liquid storage hole, F, sample holes, 1, first first adjusting channel, 2, second the
One adjusts channel, 3, third first adjust channel, 4, main channel, 5, second adjusts channel, 6, difference amplifier, 7, NI data
Capture card, 8, computer.
Specific implementation mode
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached in the embodiment of the present invention
Figure, is clearly and completely described technical scheme of the present invention, it is clear that described embodiment is that a part of the invention is real
Example is applied, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creation
Property labour under the premise of the every other embodiment that is obtained, shall fall within the protection scope of the present invention.
Technical background：When flowing through the micropore there are electric field due to particle, apparent disturbance can be generated to electric field, and it is corresponding
Both end voltage changes and generates voltage pulse signal at micropore；It, can be by detecting voltage pulse signal based on above-mentioned principle
Number realizes the purpose of the number of detection sample particle.In addition, using multiple main channels in the electric field and even or reduce lead
The length in channel can reduce the equivalent resistance of main channel, can be effectively to increase the average field intensity of sense channel
Improve accuracy of detection.
Based on above-mentioned design background, as shown in Figure 1, the present invention devises a kind of adjustable grain count dress of accuracy of detection
It sets comprising PDMS micro-fluidic chips, signal amplification circuit and Signal acquiring and processing unit, the PDMS micro-fluidic chips
To be carved with the side of microchannel in PDMS substrate M fovea superiors and glass slide L is packaged as a whole, with formed circulate for sample to be tested it is micro-
The micro-fluidic chip in channel, which is characterized in that the microchannel includes：
There are sample holes, the other end to have waste liquid hole for main channel, one end；Multiple first adjust channel, and described first adjusts
Saving channel one end, there is the first liquid storage hole, the other end to intersect with the main channel and prolong to the direction far from the main channel
It stretches；
And second adjust channel, one end has the second liquid storage hole, and the other end is connected with detection mouth and perpendicular to institute
Main channel and of same size with the main channel is stated, the detection mouth is located to be adjusted apart from the main channel and described poly- second
On the main channel of channel intersection certain distance；
Wherein, it is inserted with platinum electrode in the sample holes, waste liquid hole, each first liquid storage hole and the second liquid storage hole；And
So that the platinum electrode in second liquid storage hole is connected by the cathode of a reference resistance and DC power supply, the sample holes are given up
Platinum electrode in fluid apertures, each first liquid storage hole is connect with the anode of above-mentioned DC power supply；To generate stabilization in channel
And be gathered in the electric field of detection mouth so that sample between sample holes and waste liquid hole under pressure differential from primary passageway stream to waste liquid
Hole；The both ends of the reference resistance are connect by conducting wire with the input terminal of the signal amplification circuit simultaneously, and the signal is put
The output end of big circuit is connect with the Signal acquiring and processing unit；The Signal acquiring and processing unit includes NI capture cards
And computer.
Further, as the preferred of the present invention：
The testing principle is led to by adjusting the quantity adjusting accuracy of detection in channel in adjusting and being adjusted by change first
The quantity in road adjusts the grain count device accuracy of detection.
Further, as the preferred of the present invention：
The testing principle is logical by changing the second adjusting by adjusting the length adjustment accuracy of detection in channel under adjusting
Grain count device accuracy of detection described in the length adjustment in road.
Another object of the present invention is to provide for a kind of method carrying out grain count based on above-mentioned grain count device, special
Sign is, includes the following steps：
S1, sample are added dropwise：PBS buffer solution is added dropwise in first liquid storage hole in sample holes and 3 first adjusting channels first,
PBS buffer solution is added dropwise in lower the second liquid storage hole for adjusting channel and respectively in waste liquid hole, sample to be tested is then added dropwise to institute
It states in sample holes；
S2, sample transport：DC power supply is connected, and adjusts liquid level in each liquid storage hole, makes to wait in the sample holes
Sample acts in pressure and flows into main channel, and adjusts at 3 first and adherent in main channel to be flowed through under the differential pressure action in channel
Waste liquid hole is flow to after detection mouth；
S3, signal amplify collection analysis：Acquire particle stream after testing mouth when reference resistance both ends voltage pulse signal, and
After collected signal is amplified, is recorded by the Signal acquiring and processing unit and show that corresponding detection data is examined
The number of sample particle.
It is detected by taking polystyrene sample particle as an example below, as shown in Figure 2：
The device parameter of the implementation case：5*10 μm of the size (wide × high) of chip detection mouth used in the implementation case,
Distance among each liquid storage hole to chip main channel is 5mm, and main microchannel size is 200*10 μm (wide × high), main channel import
Size is 10*10 μm (wide × high)；Detected sample is 1 μm of granules of polystyrene solution；Buffer solution is PBS (1 ×) solution；It applies
The electric field strength for being added on channel positive and negative anodes is 20V/cm (being directed to 1 μm of particle)；
The microchannel of the device include first the first liquid storage hole A being located on the micro-fluidic chip, second first
Liquid storage hole B, third the first liquid storage hole C, waste liquid hole D, the second liquid storage hole E, sample holes F, first first adjust channel 1, the
Two first adjust channel 2, the first adjusting of third channel 3, the adjusting channel 5 of main channel 4 and second；In each liquid storage hole
It is inserted with platinum electrode；And connecting power cathode after making platinum electrode series resistance R in the second liquid storage hole E, remaining electrode connects
Connect positive pole；In two input terminals that the both ends resistance R pass through two conductor in parallel difference amplifiers 6；Difference amplifier it is defeated
Outlet is connected to the input terminal of NI data collecting cards 7；NI output signals directly can show and carry out in the computer 8 connected
Analysis.
Based on the method that above-mentioned grain count device carries out grain count, include the following steps：
S1, sample are added dropwise：The PBS buffer solution of 30 μ L is added dropwise respectively in sample holes F and each first liquid storage hole first,
10 μ L and 5 μ L PBS buffer solution are added dropwise in second liquid storage hole E and waste liquid hole D respectively, are then added dropwise to the sample to be tested of 2.5 μ L
Into sample holes F；
S2, sample transport：DC power supply is connected, liquid level in each liquid storage hole is adjusted, the sample in sample holes F is enable to exist
Pressure effect flow into main channel 4, then it is each first adjust channel 1,2 and 3 differential pressure action under in main channel 4 sticking wall flow
It crosses detection mouth and then passes to waste liquid hole D；Since there are application electric field, upstream master in each first adjusting channel 1,2,3 and main channel 4
Channel can be because circuit in parallel makes equivalent resistance greatly reduce, so that component voltage greatly increases in sense channel；When exhausted
When the particle of edge is by (vertically cutting through) detection mouth region, script size with regard to very little detection mouth region because conductive region into one
Step is reduced and equivalent resistance further increases, and so that sense channel component voltage is changed, and then the voltage at the both ends resistance R is caused to become
Change, generate detection signal, detection signal is input to NI capture cards after the amplification of AD620 difference amplifier differential signals, and NI is adopted
Truck output signal directly can show and be analyzed on the computer connected；
S3, signal amplify collection analysis：By acquire particle stream after testing mouth when reference resistance both ends voltage pulse letter
Number, after collected signal is amplified by difference amplifier, is recorded by signal gathering unit and show corresponding testing number
According to, that is, detect sample particle number.
Corresponding testing result：Detection and count results in real time can be directly obtained by computer, particle in sample
Number is equal to the number of pulse signal.
In conclusion the adjustable grain count method and apparatus of a kind of accuracy of detection of the present invention, were detected first
Cheng Zhong, the candidate particles enter main channel from sample channel, and then the adherent domain of mouth region after testing flow to waste liquid hole, just can realize
Detection and counting；Since the electric field strength of detection mouth is sufficiently large, and particle is not necessarily to enter sense channel, effectively increases detection essence
It spends and avoids channel block phenomenon.Secondly, in detection process, just using three or more first adjusting channel connection power supplys
The method of pole is by realizing that the parallel connection of fluid circuit reduces the equivalent resistance of upstream passageway, or the length in the second adjusting of reduction channel
Also it can reduce the effective resistance of downstream passage, and then increase the average field intensity and partial pressure of sense channel, detection can be improved
Precision, you can to realize detection and counting under different sized particles or different signal-to-noise ratio requirements on demand.It is worst, pass through increase
Or reduce applied voltage value, accuracy of detection can be increased or decreased — that is, can realize the detection and counting of different sized particles, or
Person meets the needs of different detection signal-to-noise ratios.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (4)
1. a kind of adjustable grain count device of accuracy of detection, the device include PDMS micro-fluidic chips, signal amplification circuit with
And Signal acquiring and processing unit, the PDMS micro-fluidic chips are side and the load glass that microchannel is carved in PDMS substrate fovea superiors
Piece is packaged as a whole, to form the micro-fluidic chip for the microchannel circulated for sample to be tested, which is characterized in that the microchannel packet
It includes：
There are sample holes, the other end to have waste liquid hole for main channel, one end；Multiple first adjust channel, and first adjusting is logical
There is road one end the first liquid storage hole, the other end to intersect with the main channel and extend to the direction far from the main channel；
And second adjust channel, one end has the second liquid storage hole, and the other end is connected with detection mouth and perpendicular to the master
Channel is simultaneously of same size with the main channel, and the detection mouth is located at adjusts channel phase apart from the main channel and described first
At friendship on the main channel of certain distance；
Wherein, it is inserted with platinum electrode in the sample holes, waste liquid hole, each first liquid storage hole and the second liquid storage hole；And make
Platinum electrode in second liquid storage hole is connected by the cathode of a reference resistance and DC power supply, the sample holes, waste liquid hole,
Platinum electrode in each first liquid storage hole is connect with the anode of above-mentioned DC power supply；The both ends of the reference resistance are logical simultaneously
Conducting wire is crossed to connect with the input terminal of the signal amplification circuit, the output end of the signal amplification circuit and the signal acquisition with
Processing unit connects.
2. the adjustable grain count device of accuracy of detection according to claim 1, it is characterised in that：
The grain count device accuracy of detection is adjusted by changing the quantity that first adjusts channel.
3. the adjustable grain count device of accuracy of detection according to claim 1, it is characterised in that：
By changing grain count device accuracy of detection described in the length adjustment that second adjusts channel.
4. a kind of adjustable grain count device of accuracy of detection based on described in claim 1-3 any one carries out grain count
Method, which is characterized in that include the following steps：
S1, sample are added dropwise：PBS buffer solution is added dropwise in first liquid storage hole in sample holes and each first adjusting channel first,
PBS buffer solution is added dropwise respectively in second liquid storage hole in the second adjusting channel and waste liquid hole, sample to be tested is then added dropwise to institute
It states in sample holes；
S2, sample transport：DC power supply is connected, and adjusts liquid level in each liquid storage hole, makes to wait for test sample in the sample holes
Product act in pressure and flow into main channel, and under the differential pressure action for adjusting channel first in main channel sticking wall flow channel after the adjustment
Detection mouth after flow to waste liquid hole；
S3, signal amplify collection analysis：Acquire particle stream after testing mouth when reference resistance both ends voltage pulse signal, and will adopt
After the signal amplification collected, is recorded by the Signal acquiring and processing unit and show that corresponding detection data detects sample
The number of product particle.
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| CN201810265887.5A CN108279202B (en) | 2018-03-28 | 2018-03-28 | Particle counting device and method with adjustable detection precision |
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| Application Number | Priority Date | Filing Date | Title |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110553956A (en) * | 2019-09-27 | 2019-12-10 | 东莞东阳光医疗智能器件研发有限公司 | impedance pulse particle detection device, detection system and detection method |
| CN111413264A (en) * | 2020-04-02 | 2020-07-14 | 华中农业大学 | Multi-channel particle detection device and method for detecting micron particles |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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