CN204405503U - A kind of portable quick particle detection - Google Patents
A kind of portable quick particle detection Download PDFInfo
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- CN204405503U CN204405503U CN201420814539.6U CN201420814539U CN204405503U CN 204405503 U CN204405503 U CN 204405503U CN 201420814539 U CN201420814539 U CN 201420814539U CN 204405503 U CN204405503 U CN 204405503U
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Abstract
The utility model relates to a kind of portable quick particle detection, and this device can detection of particles, bacterium and other biological or non-biological particles.This portable quick particle detection comprises: mount pad and the adapter being provided with detection of particulates chip; After adapter loads mount pad, the detection of particulates microchannel in detection of particulates chip forms certain angle of inclination, makes sample to be checked be suitable for flowing along detection of particulates microchannel under gravity; The detection mouth of detection of particulates microchannel is provided with the signal acquisition circuit for detection of particles size and concentration, and signal amplifies and computing module.On the basis of low cost, improve the detection efficiency of particulate; Type of drive under utilizing the effect of gravity added electric field to guide, reduces chip design complexity, decreases complicated drive unit simultaneously, reduces system difficulty and cost, improve portability; The architecture mode adopting examination and analysb to be separated, can set up detection system fast.
Description
Technical field
The utility model relates to medical detection field, particularly the portable quick particle detection of one.
Background technology
Now, all routine blood test detection techniques are all main equipment based on streaming particulate art or bench device, also do not occur can be commercial small-sized detection of particulates equipment; And its shape principle all make use of complicated type of drive, is all adopt independently integrated detection system, the disposable purchase cost of the equipment that increases.
But that detects at current third party develops rapidly, and family is detected with under the situation of the quick increase of medical demand, and to the cost of product, detection speed, equipment convenience etc. is proposed higher requirement.
Utility model content
The purpose of this utility model is to provide a kind of portable quick particle detection, to solve the technical matters that low cost particulate detects fast, counts.
In order to solve the problems of the technologies described above, the utility model provides a kind of portable quick particle detection, comprising: mount pad and the adapter being provided with detection of particulates chip; After described adapter loads mount pad, the detection of particulates microchannel in described detection of particulates chip forms certain angle of inclination, makes sample to be checked be suitable under gravity along the flowing of described detection of particulates microchannel; The detection mouth of described detection of particulates microchannel is provided with the counting module for detection of particulates.
Preferably, described detection of particulates chip comprises: substrate and chip section in the form of sheets; Described chip section is provided with some through holes, and the upper surface of described substrate and the lower surface of described chip section fit tightly, and form corresponding liquid storage tank to each through hole; Described in several, detection of particulates microchannel is positioned at the upper surface of described substrate, each detection of particulates microchannel is suitable for connecting corresponding liquid storage tank respectively, wherein arbitrary liquid storage tank is sample input port, and the particulate sample to be checked in this liquid storage tank presses gravity direction and/or direction of an electric field flowing along detection of particulates microchannel.
Preferably, described adapter comprises: circuit board, and gap is left in the lower surface of this circuit board and the upper surface of described chip section, and described lower surface is also provided with the contact pilotage that some are suitable for stretching into respectively chip liquid storage tank, and this contact pilotage is platinum gold, silver or other noble metals.These contact pilotages by the expanding channels in liquid storage tank and detection of particulates chip to realize electric field driven and signals collecting.
Preferably, described mount pad is provided with the socket being suitable for adapter and tilting to insert, and tilt angle alpha is 15 ° ~ 90 °.
Preferably, in order to improve the identification sensitivity of counting module further, described counting module comprises: two signals collecting ends being relatively arranged on described detection mouth both sides, two voltage signal acquisition ends are connected with two input ends of signal acquisition circuit respectively, and the output terminal of this signal acquisition circuit is connected with the signal input part of a processor module, described processor module is suitable for counting the pulse signal of voltage signal acquisition end and Cytometric Analysis (namely draws particle size distribution, the concentration of different size particles, the result of calculation of total number of particles).
Preferably, some contact pilotages comprise: be suitable for providing the contact pilotage of bias voltage and the contact pilotage of collection signal; Wherein form the positive voltage V+ of bias voltage, the common point of negative voltage V-and signal acquisition circuit altogether; And the contact pilotage of collection signal is connected with signals collecting end.
Preferably, described signal acquisition circuit comprises the first, second, third grade of amplifying unit connected successively; Described first order amplifying unit comprises: first order differential amplifier circuit, and the first order bandpass filter be connected with the output terminal of this first order differential amplifier circuit; The output terminal of described first order bandpass filter is connected with second level amplifying unit; Described second level amplifying unit comprises: second level operational amplification circuit, and the second level low-pass filter be connected with the output terminal of this second level operational amplification circuit; The output terminal of described second level low-pass filter is connected with third level amplifying unit; Described third level amplifying unit comprises: third level operational amplification circuit, and the third level low-pass filter be connected with the output terminal of this third level operational amplification circuit; The output terminal of described second, third grade of low-pass filter is respectively as the output terminal of signal acquisition circuit.
Preferably, the output terminal of described second level low-pass filter is connected with described processor module by a voltage follower.
Preferably, first order bandpass filter be 0.5-60Hz by frequency, the cutoff frequency of second level low-pass filter is 10-60Hz, and the cutoff frequency of third level low-pass filter is 10-60Hz.
On the other hand, the utility model additionally provides a kind of method of work of portable quick particle detection, to solve the technical matters that low cost particulate detects fast, counts.
In order to solve the problems of the technologies described above, the utility model provides a kind of method of work of portable quick particle detection, comprises the steps:
Step S100, microchannel to be detected is full of electrolytic liquid, forms conductive path; And form the path of fluid.
Step S200, puts into the liquid storage tank of specifying by sample to be checked, by electric field driven or gravity traction, sample to be checked is flowed along detection of particulates microchannel.
Step S300, by being connected to the contact pilotage collection signal of the detection mouth of described detection of particulates microchannel, and carries out grain count, to draw particle size distribution, and the concentration of different size particles, the result of calculation of total number of particles.
Preferably, in order to improve the identification sensitivity of counting module further, tally function in described step S300 adopts counting module, this counting module comprises: signal acquisition circuit, described signal acquisition circuit comprises: first, second, third amplifying unit connected successively, the wherein gain A of first order amplifying unit
1, the gain A of second level amplifying unit
2, the gain of third level amplifying unit is A
3, namely the full gain of described signal acquisition circuit is A
gain=A
1a
2a
3.
Preferably, the output data of described second level amplifying unit are the particle that diameter is larger, and the output data of third level amplifying unit are the particle that diameter is less; Described processor module is suitable for counting the pulse signal of voltage signal acquisition end, to realize detecting in the mixing sample being mixed with different-diameter particle the particle that diameter is larger.
Further, the diameter range of the particle that described diameter is larger is 5 microns-35 microns, and the diameter range of the particle that diameter is less is 0.5 micron-5 microns.
Preferably, described processor module is suitable for counting the pulse signal of voltage signal acquisition end, to realize detecting that diameter is for 520nm particle in the mixing sample that diameter is 1um and 520nm particle being mixed with.
The beneficial effects of the utility model are: the utility model, on the basis of low cost, improves the detection efficiency of particulate; Particulate type of drive under utilizing the effect of gravity added electric field to guide, reduces the complexity of chip design and circuit design, decreases complicated drive unit simultaneously, reduce further system difficulty and cost, and improve portability; The architecture mode that the utility model also adopts examination and analysb to be separated, can set up detection system anywhere easily and fast.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is further illustrated.
Fig. 1 shows the structural representation of detection of particulates chip in portable quick particle detection;
Fig. 2 shows the structural representation of the optimum embodiment of adapter of the present utility model;
Fig. 3 shows the exploded perspective view that detection of particulates chip of the present utility model and adapter are installed;
Fig. 4 shows the structural representation of adapter housing of the present utility model;
Fig. 5 shows the structural representation that in adapter of the present utility model, circuit board and chip are assembled;
Fig. 6 shows the structural representation that in adapter of the present utility model, circuit board and chip are assembled;
Fig. 7 shows the structural representation of pedestal in mount pad of the present utility model;
Fig. 8 shows the structural representation of compressing tablet in mount pad of the present utility model;
Fig. 9 show mount pad of the present utility model and adapter install after structural representation;
Figure 10 show mount pad of the present utility model and adapter install after exploded perspective view;
Figure 11 show mount pad of the present utility model and adapter install after planar structure schematic diagram;
Figure 12 shows the schematic block circuit diagram of counting module of the present utility model and the first signal acquisition circuit;
Figure 13 shows the voltage signal of the generation for 1um particle and 520nm particle.
Wherein, bottom substrate 1, chip section 2, liquid storage tank 21, circuit board 3, contact pilotage 31, contact 32, unfilled corner shape 33, shell 4, mounting groove 41, hypotenuse 411, construction opening 412, step 42, groove 43, through hole 44, pedestal 100, window 101, pedestal 102, socket 103, erecting bed 104, compressing tablet 200, metal clips 201, fabricated section 300, adapter 400, detection of particulates microchannel 500, tested particle 501, voltage signal acquisition end 601.
Embodiment
By reference to the accompanying drawings the utility model is described in further detail now.These accompanying drawings are the schematic diagram of simplification, only basic structure of the present utility model are described in a schematic way, and therefore it only shows the formation relevant with the utility model.
Embodiment 1
Portable quick particle detection of the present utility model, comprising: mount pad and the adapter 400 being provided with detection of particulates chip; After described adapter 400 loads mount pad, the detection of particulates microchannel 500 in described detection of particulates chip forms certain tilt angle alpha, makes sample to be checked be suitable for flowing along described detection of particulates microchannel 500 under gravity; The detection mouth of described detection of particulates microchannel 500 is connected with the counting module for detection of particulates.
Fig. 1 shows the structural representation of detection of particulates chip in portable quick particle detection.
Figure 12 shows the schematic block circuit diagram of counting module of the present utility model and the first signal acquisition circuit.
As shown in figs. 1 and 12, described detection of particulates chip comprises: substrate 1 and chip section in the form of sheets 2, described chip section 2 is provided with some through holes, the upper surface of described substrate 1 fits tightly to the lower surface of described chip section 2 and forms corresponding liquid storage tank 21 with each through hole, detection of particulates microchannel 500 described in several is positioned at the upper surface of described substrate 1, each detection of particulates microchannel 500 is suitable for connecting corresponding liquid storage tank 21 respectively, wherein arbitrary liquid storage tank is sample input port, particulate sample to be checked in liquid storage tank 21 is flowed out along detecting microchannel 500 by gravity direction or direction of an electric field, tested particle 501 in described sample to be checked is successively through detecting mouth.Liquid storage tank 21 can provide the input of sample microparticles, also provides the output of detection signal.These liquid storage tanks 21 are interconnected to form specific network by passage, thus realize the flowing of sample in passage and detect.
Wherein, tested particulate can include but not limited to the various fine particles such as fine-grained particles, such as cell and other biological or non-biological particles.
Optionally, the base material of described substrate 1 and chip section 2 is such as but not limited to plastic sheet, glass sheet, piezoid or silicon chip, and by etching, mould is noted, and the modes such as photoetching, form described detection of particulates microchannel on the substrate 1.
Fig. 2 shows the structural representation of the optimum embodiment of adapter of the present utility model.
Fig. 3 shows the exploded perspective view that detection of particulates chip of the present utility model and adapter are installed.
Fig. 4 shows the structural representation of adapter housing of the present utility model.
Fig. 5 shows the structural representation that in adapter of the present utility model, circuit board and chip are assembled.
Fig. 6 shows the structural representation that in adapter of the present utility model, circuit board and chip are assembled.
As shown in Figures 2 to 6, described adapter comprises: circuit board 3, and gap is left in the lower surface of described circuit board 3 and the upper surface of described chip section 2, and described lower surface is also provided with the contact pilotage 31 that some are suitable for stretching into respectively chip liquid storage tank.
Optionally, described contact pilotage is platinum gold, silver or other noble metals, and these contact pilotages, by the expanding channels in liquid storage tank and detection of particulates chip, realize electric field driven, the functions such as signals collecting.
Concrete, adapter of the present utility model, comprise circuit board 3 and shell 4, mounting groove 41 is provided with in described shell 4, described substrate 1, chip section 2 and circuit board 3 are successively set in the mounting groove 41 of described shell 4 from bottom to up, and the described through hole that described chip section 2 offers is corresponding with the position of contact pilotage 31; After described circuit board 3 is installed with chip section 2, described contact pilotage 31 stretches in described liquid storage tank 21; The upper surface of described circuit board 3 is provided with several contacts 32 be connected with late-class circuit, several contacts 32 described respectively with some contact pilotages 31 connection, contact 32 is disposed side by side on one end edge of circuit board 3.Described substrate 1 fits tightly with chip section 2 and forms described detection of particulates chip, and sample to be checked flows and realizes signals collecting in described detection of particulates microchannel 500.Only need sample to be checked to enter in detection of particulates microchannel 500, then circuit board 3 is placed in mounting groove 41, just can detect fast.Wherein, described contact pilotage 31 is suitable for measuring the parameter of the sample to be checked in liquid storage tank 21, and these parameters include but not limited to the parameters such as temperature, pH value, ion concentration.As shown in Figure 3, described shell 4 upper opening, bottom is described mounting groove 41, and described mounting groove 41 has construction opening 412, and described construction opening 412 place has step 42; The wherein two relative sides of described shell 4 all offer groove 43; Through hole 44 is also offered bottom described shell 4.
The mounting groove 41 of described circuit board 3 and shell 4 is equipped with fool proof unit, the fool proof unit of described circuit board 3 is that circuit board 3 one jiaos of outer side edges form unfilled corner shape 33, and the fool proof unit on described mounting groove 41 is hypotenuse 411 corresponding with described unfilled corner shape 33 on the inner side edge of mounting groove 41.
Fig. 7 shows the structural representation of pedestal in mount pad of the present utility model.
Fig. 8 shows the structural representation of compressing tablet in mount pad of the present utility model.
Fig. 9 show mount pad of the present utility model and adapter install after structural representation.
Figure 10 show mount pad of the present utility model and adapter install after exploded perspective view.
Figure 11 show mount pad of the present utility model and adapter install after planar structure schematic diagram.
As shown in Fig. 7 to Figure 11, described mount pad is provided with the socket 103 being suitable for adapter 400 and tilting to insert, and tilt angle alpha is 15 ° ~ 90 °.
Concrete, mount pad of the present utility model, comprise pedestal 100, described pedestal 100 can place with bottom level or level is installed, described pedestal 100 is provided with socket 103, be downwards the erecting bed 104 of adapter along described socket 103, bottom erecting bed 104 and pedestal, the tilt angle alpha of 102 is 15 ° ~ 90 °.Wherein, described tilt angle alpha is the angle of inclination of erecting bed 104 and surface level, concrete, after adapter 400 inserts erecting bed 104, detection of particulates microchannel 500 in the detection of particulates chip of this adapter 400 inside also forms an angle of inclination with surface level, and this angle of inclination is described tilt angle alpha.
Preferably, described tilt angle alpha is 45 ° or 60 °.Under these two kinds of angle case, tested sample can pass through gravity control sample flowing velocity, is namely conducive to particle and is focused in flow process, is also conducive to the high flux detected, and is that our a kind of design optimization is selected.
Described pedestal 100 is positioned at correspondence above erecting bed 104 and offers window 101, described window 101 is near the tilting bottom of described erecting bed 104, in described window 101, compressing tablet 200 is installed, as shown in Figure 8, described compressing tablet 200 has several metal clips 201 be arranged side by side towards the one side of erecting bed 104.Described compressing tablet 200 is fixed by the fabricated section 300 that is pressed on its two ends, and to reach the object of contact stabilization, the design of metal clips 201 ensure that the abundant contact of the contact 32 of adapter 400, and metal clips 201 adopts click-on design, is convenient to dismounting and maintenance.
Described adapter 400 inserts from the socket 103 pedestal 100, be plugged on adapter erecting bed 104 downwards along described socket 103, circuit board 3 upward, and several contacts 32 of circuit board 3 are positioned at bottom, several metal clips 201 on the compressing tablet 200 on mount pad of the present utility model contact with several contacts 32 of the circuit board 3 of adapter 400 respectively.
Described adapter 400 adopts angle-inserting type, utilize gravity to drive the tested particle in sample to be tested, gathering and the focusing of tested particle can be realized, ensure that surveyed area is that single sample successively passes through, achieve high-precision detection, ensure that detection flux simultaneously.
Detection of particulates chip of the present utility model and adapter thereof, because circuit board is separated with chip, and the consumptive material of circuit board non-once, one piece of circuit board can meet thousands of secondary detection, and each testing process only needs to change chip, and chip is medical disposable material, processing cost is lower, greatly reduce the testing cost of single, chip is recycled as plastic product is convenient, has good environment-friendly advantage.Whole operation is very simple, without the need for professional and technical personnel, and device miniaturization, can use under various movement or non-moving application conditions.
Figure 12 shows the schematic block circuit diagram of counting module of the present utility model and the first signal acquisition circuit.
As shown in figure 12, described counting module comprises: two voltage signal acquisition ends being relatively arranged on described detection mouth both sides, two voltage signal acquisition ends are connected with two input ends of signal acquisition circuit respectively, and the output terminal of this signal acquisition circuit is connected with the signal input part of a processor module, described processor module is suitable for counting the pulse signal of voltage signal acquisition end and analyzing.The implication of analysis described here is classified by diameter by the particulate of different-diameter particle in sample according to Coulter principle.
Described signal acquisition circuit can adopt following embodiment.
As shown in figure 12, described signal acquisition circuit comprises the first, second, third grade of amplifying unit connected successively; Described first order amplifying unit comprises: first order differential amplifier circuit, and the first order bandpass filter be connected with the output terminal of this first order differential amplifier circuit; The output terminal of described first order bandpass filter is connected with second level amplifying unit; Described second level amplifying unit comprises: second level operational amplification circuit, and the second level low-pass filter be connected with the output terminal of this second level operational amplification circuit; The output terminal of described second level low-pass filter is connected with third level amplifying unit; Described third level amplifying unit comprises: third level operational amplification circuit, and the third level low-pass filter be connected with the output terminal of this third level operational amplification circuit; The output terminal of described second, third grade of low-pass filter is respectively as the output terminal of signal acquisition circuit.
Preferably, the output terminal of described second level low-pass filter is connected with described processor module by a voltage follower.
Further, first order bandpass filter be 0.5-60Hz by frequency, the cutoff frequency of second level low-pass filter is 10-60Hz, and the cutoff frequency of third level low-pass filter is 10-60Hz.
The output terminal of described signal acquisition circuit is also suitable for being positioned at the low-pass filter of output terminal by one or multiple filter is connected with the signal input part of described processor module, and described bandpass filter or multiple filter is 0.5-60Hz by frequency, the cutoff frequency of low-pass filter is 10-60Hz.Preferably, according to particle size, the tractive force size that chip setting angle is formed, can impact the speed of particulate by check point, this impact is exactly the frequency that particulate signal occurs and the time at every turn occurred, therefore, optimum is 5-60Hz by frequency.
Optionally, described first order differential amplifier circuit selects AD620, and OP07 is selected in the second level, third level operational amplification circuit.
The differential gain is improved to improve the identification sensitivity of described counting module, the noise signal that this counting module utilizes the differential amplifier circuit in described signal acquisition circuit to introduce to offset detection chip in order to solve.
Figure 13 shows the voltage curve for diameter to be 1um particle and diameter the be generation of 520nm particle.
The technical scheme of this signal acquisition circuit solves the technical matters detecting 520nm particle in the suspending liquid being mixed with 1um particle and 520nm particle.As shown in figure 13, diameter is the about 300mV of peak value of 520nm particle, and this is with regard to differentiation clearly and the baseline being highly 100mV.520nm particle, under magnification at high multiple, can obtain the high s/n ratio of 9.54dB.The peak value of 1um particle can bring up to 2.4V, and the height of baseline only 100mV.Therefore, the signal to noise ratio (S/N ratio) of our improvement is from 9.54 to 27.6 decibels.Wherein, each grade of differential amplifier and low-pass filter are depended in the reduction of noise.For 1um and 520 nm particles, the ratio 8:1(2.4 V:300 MV of its signal intensity) approximate the volume ratio of particle, therefore, there is good linear relationship in the volume of particle and signal intensity.Further, the particle of 520nm represents, the volume ratio 0.0004% of this particle and detect aperture, (according to technical specification Coulter Multisizer 3; Www.beckmancoulter.com/ms3.The dynamic range of detect aperture is 27000:1, and this corresponds to volume ratio and is approximately 0.0037%).
As shown in figure 12, the gain A of first order amplifying unit
1, the gain A of second level amplifying unit
2, the gain of third level amplifying unit is A
3, namely the full gain of described signal acquisition circuit is A
gain=A
1a
2a
3.
Therefore, described counting module adopts this signal acquisition circuit, and noise has had obvious reduction; Further, further by adopting the common mode inhibition of unique binary channels design, multistage amplification and low-pass filter or multiple filter.Two-stage amplifies the rear minimum particle detected and detect aperture volume ratio is 0.0004%.
Optionally, described processor module is also connected with TCP/IP, USB2.0, the communication interfaces such as RS485, and the computing machine being suitable for holding with PC carries out data transmission, and receives the operational order of PC end.
Described computing machine can hold intellectual analysis software systems to be added up by the data obtained front end according to PC, analyze, according to different test sample books and test purpose, according to built-in reduced parameter, in conjunction with auxiliary rash treatment functions, provide test result and reference proposition, and according to customer demand, form the printout of standard format.
Described processor module can adopt 51 series monolithics, or STC series monolithic.
Embodiment 2
On embodiment 1 basis, the utility model additionally provides a kind of method of work of portable quick particle detection, comprises the steps:
Step S100, microchannel to be detected is full of electrolytic liquid, forms conductive path; To form the path of fluid;
Step S200, puts into arbitrary liquid storage tank by sample to be checked, by electric field driven and/or gravity traction, sample to be checked is flowed along detection of particulates microchannel; And
Step S300, by being connected to the contact pilotage collection signal of the detection mouth of described detection of particulates microchannel, and carries out counting micro particles, to draw particle size distribution, and the concentration of different size particles, the result of calculation of total number of particles.
Tally function in described step S300 adopts counting module, and this counting module comprises: signal acquisition circuit, and described signal acquisition circuit comprises: first, second, third amplifying unit connected successively, the wherein gain A of first order amplifying unit
1, the gain A of second level amplifying unit
2, the gain of third level amplifying unit is A
3, namely the full gain of described signal acquisition circuit is A
gain=A
1a
2a
3.About the corresponding contents in the embodiment detailed in Example 1 of counting module.
The output data of described second level amplifying unit are the particle that diameter is larger, and the output data of third level amplifying unit are the particle that diameter is less; Described processor module is suitable for counting the pulse signal of voltage signal acquisition end, to realize detecting in the mixing sample being mixed with different-diameter particle the particle that diameter is larger.
The diameter range of the particle that described diameter is larger is 5 microns-35 microns, and the diameter range of the particle that diameter is less is 0.5 micron-5 microns.
Described processor module is suitable for implementation in and is mixed with diameter is detect that diameter is 520nm particle in the mixing sample of 1um and 520nm particle.
Below the experimental data of the utility model for particulate.
The laboratory test results of portable quick particle detection: the detection of 4 kinds of different size particles mixing samples.Wherein, 4 kinds of particle diameter (region) sizes are respectively: 2.94um, 5.09um, 7.32um and 9.75um, 1.00um, 520nm, blending ratio is unknown in advance.Six kinds of grain sizes cover from blood platelet to the physical size of white particulate in whole blood particulate, therefore can be detected the basic technical indicator of the application's equipment completely by the experiment of such mixing sample.
The testing result contrast table of this portable quick particle detection and streaming particulate instrument BD facscalibur is as follows:
The testing result contrast table of the portable quick particle detection of table 1 and streaming particulate instrument
Confirmatory experiment can be found out, this portable quick particle detection detects the number percent of six kinds of particles is 4.07%, 5.78%, 29.43%, 55.26%, 11.01%, 5.46%, and commercial streaming particulate instrument detects same sample, the number percent of the six kinds of particles obtained is 4.32%, 5.33%, 25.86%, 59.55%, 10.74%, 4.94%.As can be seen from the results, the test findings of this portable quick particle detection can be compared with commercial streaming particulate instrument completely, and reaches higher accuracy rate.
The utility model not only can be used for detection of particles, can also be used for detecting, identifying other fine particles.
With above-mentioned according to desirable embodiment of the present utility model for enlightenment, by above-mentioned description, relevant staff in the scope not departing from this utility model technological thought, can carry out various change and amendment completely.The technical scope of this utility model is not limited to the content on instructions, must determine its technical scope according to right.
Claims (9)
1. a portable quick particle detection, is characterized in that, comprising: mount pad and the adapter being provided with detection of particulates chip;
After described adapter loads mount pad, the detection of particulates microchannel in described detection of particulates chip forms certain angle of inclination, makes sample to be checked be suitable under gravity along the flowing of described detection of particulates microchannel;
The detection mouth of described detection of particulates microchannel is connected with the counting module for detection of particulates.
2. portable quick particle detection according to claim 1, is characterized in that, described detection of particulates chip comprises: substrate and chip section in the form of sheets; Described chip section is provided with some through holes, and the upper surface of described substrate and the lower surface of described chip section fit tightly, and form corresponding liquid storage tank to each through hole; Described in several, detection of particulates microchannel is positioned at the upper surface of substrate, each detection of particulates microchannel is suitable for connecting corresponding liquid storage tank respectively, wherein arbitrary liquid storage tank is sample input port, and the particulate sample to be checked in this liquid storage tank presses gravity direction and/or direction of an electric field flowing along detecting microchannel.
3. portable quick particle detection according to claim 2, it is characterized in that, described adapter comprises: circuit board, gap is left in the lower surface of this circuit board and the upper surface of described chip section, described lower surface is also provided with the contact pilotage that some are suitable for stretching into respectively chip liquid storage tank, this contact pilotage by the expanding channels in liquid storage tank and detection of particulates chip to realize electric field driven and signals collecting.
4. portable quick particle detection according to claim 3, is characterized in that, described mount pad is provided with the socket being suitable for adapter and tilting to insert, and tilt angle alpha is 15 ° ~ 90 °.
5. portable quick particle detection according to claim 1, it is characterized in that, described counting module comprises: two signals collecting ends being relatively arranged on described detection mouth both sides, two voltage signal acquisition ends are connected with two input ends of signal acquisition circuit respectively, and the output terminal of this signal acquisition circuit is connected with the signal input part of a processor module, described processor module is suitable for counting and Cytometric Analysis the pulse signal of voltage signal acquisition end.
6. portable quick particle detection according to claim 5, it is characterized in that, some contact pilotages comprise: be suitable for providing the contact pilotage of bias voltage and the contact pilotage of collection signal; Wherein
Form the positive voltage V+ of bias voltage, the common point of negative voltage V-and signal acquisition circuit altogether; And the contact pilotage of collection signal is connected with signals collecting end.
7. portable quick particle detection according to claim 5, is characterized in that, described signal acquisition circuit comprises the first, second, third grade of amplifying unit connected successively;
Described first order amplifying unit comprises: first order differential amplifier circuit, and the first order bandpass filter be connected with the output terminal of this first order differential amplifier circuit; The output terminal of described first order bandpass filter is connected with second level amplifying unit;
Described second level amplifying unit comprises: second level operational amplification circuit, and the second level low-pass filter be connected with the output terminal of this second level operational amplification circuit; The output terminal of described second level low-pass filter is connected with third level amplifying unit;
Described third level amplifying unit comprises: third level operational amplification circuit, and the third level low-pass filter be connected with the output terminal of this third level operational amplification circuit;
The output terminal of described second, third grade of low-pass filter is respectively as the output terminal of signal acquisition circuit.
8. portable quick particle detection according to claim 7, is characterized in that, the output terminal of described second level low-pass filter is connected with described processor module by a voltage follower.
9. the portable quick particle detection according to claim 7 or 8, it is characterized in that, first order bandpass filter be 0.5-60Hz by frequency, the cutoff frequency of second level low-pass filter is 10-60Hz, and the cutoff frequency of third level low-pass filter is 10-60Hz.
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| CN105510191A (en) * | 2015-11-19 | 2016-04-20 | 江苏卓微生物科技有限公司 | Flow-type particle detection method |
| CN105758782A (en) * | 2014-12-19 | 2016-07-13 | 江苏卓微生物科技有限公司 | Portable rapid particle detection apparatus and working method thereof |
| CN106198363A (en) * | 2016-09-13 | 2016-12-07 | 江苏卓微生物科技有限公司 | A kind of FCM analysis chip |
| CN108627448A (en) * | 2018-06-05 | 2018-10-09 | 江苏卓微生物科技有限公司 | The method of counting micro particles |
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2014
- 2014-12-19 CN CN201420814539.6U patent/CN204405503U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105758782A (en) * | 2014-12-19 | 2016-07-13 | 江苏卓微生物科技有限公司 | Portable rapid particle detection apparatus and working method thereof |
| CN105510191A (en) * | 2015-11-19 | 2016-04-20 | 江苏卓微生物科技有限公司 | Flow-type particle detection method |
| CN105510191B (en) * | 2015-11-19 | 2019-02-05 | 江苏卓微生物科技有限公司 | Streaming particle detection method |
| CN106198363A (en) * | 2016-09-13 | 2016-12-07 | 江苏卓微生物科技有限公司 | A kind of FCM analysis chip |
| CN108627448A (en) * | 2018-06-05 | 2018-10-09 | 江苏卓微生物科技有限公司 | The method of counting micro particles |
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