CN101776611A - Detection method for measuring sugar content in environmental water sample by spectrophotometer - Google Patents
Detection method for measuring sugar content in environmental water sample by spectrophotometer Download PDFInfo
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- CN101776611A CN101776611A CN201010108638A CN201010108638A CN101776611A CN 101776611 A CN101776611 A CN 101776611A CN 201010108638 A CN201010108638 A CN 201010108638A CN 201010108638 A CN201010108638 A CN 201010108638A CN 101776611 A CN101776611 A CN 101776611A
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Abstract
The invention relates to a detection method for measuring sugar content in environmental water sample by a spectrophotometer, and the method comprises the following steps: sucking 1mL of filtered water sample into a tube, adding phenol solution (of the concentration of 5 percent) and concentrated sulfuric acid, stirring and placing for 10 minutes, measuring a light absorption value at 490nm position after the color is displayed fully, and measuring the sugar content in the water sample according to the curve of the concentration and the light absorption value. The method for detecting the sugar content in water is rapid and effective, and the relative average deviation is less than 5.0 percent. Therefore, the method for detecting the sugar content in the environment water sample is more reliable and is easy to implement, which can meet the requirements of research and production.
Description
Technical field
The present invention relates to a kind of method that sugared content in the environmental water sample based on glucose is detected, particularly relate to a kind of by spectrophotometric method to sugared content detecting method in the environmental water sample.
Background technology
Sugar is that the organic sphere distributed pole is wide, the class organic compound that content is bigger.All contain it in nearly all animal, plant, the microbial body.Sugar is being brought into play important physiological function in life process, be the focus of modern life science research.
Sugar in the environment water is based on glucose, and the height of sugared concentration reflects pollution situation sugared in the water quality with variation.Because the uv absorption of saccharide compound is very weak, the sensitivity of differential refraction detection method is lower again, so the analysis of carbohydrate is a difficult point of analytical chemistry always.Adopt the phenolsulfuric acid reaction with its content of carbohydrates form colorimetric estimation, its colored intensity is directly proportional with sugared content, calculates sugared content with this.
Summary of the invention
Technical matters to be solved by this invention is, sugared content detecting method in a kind of spectrophotometric determination environmental water sample is provided, and comprises the steps:
(1) preparation of typical curve:
A. glucose standard reserving solution preparation: accurately take by weighing relative molecular mass 5 * 10
5Be dried to the glucose standard items 0.5000g of constant weight, be dissolved in water, and be settled to 50mL, mixing is put in the refrigerator and is preserved.This solution contains 10.0mg glucose for every milliliter.
B. the glucose standard is used the liquid preparation: draw glucose standard reserving solution 1.0mL, place the 100mL volumetric flask, add water to scale, mixing is put in the refrigerator and is preserved.This solution 1mL contains glucose 0.10mg.
C. typical curve is prepared and determination step: get 0,0.1,0.2 respectively,, 0.4,0.6,0.8 1.0mL glucose standard uses liquid in the 25mL color-comparison tube, accurately replenishes distilled water to 1.0mL, add 5% phenol solution 1mL, mixing (noting the jog venting) adds concentrated sulphuric acid 5mL again, shake up, place 40min in room temperature, sentencing blank reagent solution with spectrophotometer in the 490nm wavelength is reference, and the 1cm cuvette is measured absorbance.Content with glucose is horizontal ordinate, and absorbance is an ordinate, the drawing standard curve.After measured, the equation of typical curve is Y=0.0093X+0.0089, linearly dependent coefficient R
2=0.9997.
(2) sample preparation: water sample is standby behind 0.45 μ m membrane filtration.
(3) mensuration of sample: the water sample after absorption 1.0mL (the concentration height should be got an amount of dilution) filters is in the 25mL color comparison tube.Add 5% phenol solution 1mL, mixing (noting the jog venting) adds concentrated sulphuric acid 5mL again, shakes up, and places 40min in room temperature, and sentencing blank reagent solution with spectrophotometer in the 490nm wavelength is reference, and the 1cm cuvette is measured absorbance.Find sugared content from typical curve, sugared content in the calculation sample.Do the sample blank experiment simultaneously.
(4) result calculates: sugar calculates with formula (1) in the water sample:
In the formula:
The content of sugar in the X-water sample, unit are every liter (mg/L) of milligram;
The concentration that checks on the m-curve, unit is microgram (μ g);
V-draws the volume (ml) of water sample.
Advantage of the present invention is: adopt the content of sugar in the spectrophotometric method testing environment water body, effectively, RSD detects and is limited to 1mg/L less than 5% fast.This shows that method of the present invention is the content of sugar in the testing environment, a kind of method of implementing be convenient to reliably be provided, can satisfy research and produce in needs.
Embodiment
Embodiment 1: the precision of total sugar content and the recovery detect in the lake water
(1) preparation of typical curve: draw 0,0.1,0.2 respectively, 0.4,0.6,0.8,1.0mL Standard for Sugars uses liquid (100 μ g/mL) in the 25mL color-comparison tube, accurately replenishes distilled water to 1.0mL, adds 5% phenol solution 1mL, mixing (noting the jog venting), add concentrated sulphuric acid 5mL again, shake up, place 40min in room temperature, sentencing blank reagent solution with spectrophotometer in the 490nm wavelength is reference, and the 1cm cuvette is measured absorbance.Content with glucose is horizontal ordinate, and absorbance is an ordinate, the drawing standard curve.After measured, the equation of typical curve is Y=0.0093X+0.0089, linearly dependent coefficient R
2=0.9997.
(2) sample preparation: the lake water sample places tool plug conical flask standby behind 0.45 μ m membrane filtration.
(3) mensuration of sample: the water sample after absorption 1.0mL (the concentration height should be got an amount of dilution) filters is in the 25mL color comparison tube.Add 5% phenol solution 1mL, mixing (noting the jog venting) adds concentrated sulphuric acid 5mL again, shakes up, and places 40min in room temperature, and sentencing blank reagent solution with spectrophotometer in the 490nm wavelength is reference, and the 1cm cuvette is measured absorbance.Find sugared content from typical curve, sugared content in the calculation sample.Do the sample blank experiment simultaneously.
Obtain six parallel sample with same processing mode, and analyze and calculate content, analysis result is as follows:
Obviously, those of ordinary skill in the art can measure sugared content in the environment water with the phenolsulfuric acid spectrophotometric method of invention.
The foregoing description is only for the usefulness that the present invention is described; and be not to be limitation of the present invention; the those of ordinary skill in relevant technologies field; without departing from the present invention; can also make various variations and modification; therefore all technical schemes that are equal to also should belong to category of the present invention, and scope of patent protection of the present invention should be limited by each claim.
Claims (5)
1. the present invention relates to sugared content detecting method in a kind of spectrophotometric determination environmental water sample, it is characterized in that: described assay method comprises the steps:
(1) preparation of typical curve:
A. glucose standard reserving solution preparation: accurately take by weighing relative molecular mass 5 * 10
5Be dried to the glucose standard items 0.5000g of constant weight, be dissolved in water, and be settled to 50mL, mixing is put in the refrigerator and is preserved.This solution contains 10.0mg glucose for every milliliter.
B. the glucose standard is used the liquid preparation: draw glucose standard reserving solution 1.0mL, place the 100mL volumetric flask, add water to scale, mixing is put in the refrigerator and is preserved.This solution 1mL contains glucose 0.10mg.
C. typical curve is prepared and determination step: get 0,0.1,0.2 respectively,, 0.4,0.6,0.8 1.0mL glucose standard uses liquid in the 25mL color-comparison tube, accurately replenishes distilled water to 1.0mL, add 5% phenol solution 1mL, mixing (noting the jog venting) adds concentrated sulphuric acid 5mL again, shake up, place 40min in room temperature, sentencing blank reagent solution with spectrophotometer in the 490nm wavelength is reference, and the 1cm cuvette is measured absorbance.Content with glucose is horizontal ordinate, and absorbance is an ordinate, the drawing standard curve.After measured, the equation of typical curve is Y=0.0093X+0.0089, linearly dependent coefficient R
2=0.9997.
(2) sample preparation: water sample is standby behind 0.45 μ m membrane filtration.
(3) mensuration of sample: the water sample after absorption 1.0mL (the concentration height should be got an amount of dilution) filters is in the 25mL color comparison tube.Add 5% phenol solution 1mL, mixing (noting the jog venting) adds concentrated sulphuric acid 5mL again, shakes up, and places 40min in room temperature, and sentencing blank reagent solution with spectrophotometer in the 490nm wavelength is reference, and the 1cm cuvette is measured absorbance.Find sugared content from typical curve, sugared content in the calculation sample.Do the sample blank experiment simultaneously.
(4) result calculates: sugar calculates with formula (1) in the water sample:
In the formula:
The content of sugar in the X-water sample, unit are every liter (mg/L) of milligram;
The concentration that checks on the m-curve, unit is microgram (μ g);
V-draws the volume (ml) of water sample.
2. sugared content detecting method in a kind of spectrophotometric determination environmental water sample according to claim 1 is characterized in that: it is 5 * 10 that standard items are selected molecular mass
5Glucose.
3. sugared content detecting method in a kind of spectrophotometric determination environmental water sample according to claim 1 is characterized in that: reagent dosage should be strict, draws water sample 1mL, adds phenol solution 1mL, adds concentrated sulphuric acid 5mL.
4. sugared content detecting method in a kind of spectrophotometric determination environmental water sample according to claim 1 is characterized in that: after adding phenolsulfuric acid solution, be 40min standing time.
5. sugared content detecting method in a kind of spectrophotometric determination environmental water sample according to claim 1 is characterized in that: use the spectrophotometer colorimetric, wavelength is 490nm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495012A (en) * | 2011-12-05 | 2012-06-13 | 中国水产科学研究院黄海水产研究所 | Method for identifying sugar-doped dried sea cucumber |
| CN105628631A (en) * | 2015-12-28 | 2016-06-01 | 天津科技大学 | Rapid detection method of biological catalytic conversion rate of hydrocortisone |
| CN110174362A (en) * | 2019-05-05 | 2019-08-27 | 贵州中烟工业有限责任公司 | A method of detection neutral sugar and acid sugared content |
| CN110174361A (en) * | 2019-05-05 | 2019-08-27 | 贵州中烟工业有限责任公司 | A kind of measurement method of total reducing sugar |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6361960B1 (en) * | 1999-11-09 | 2002-03-26 | Environmentally Sensitive Solutions, Inc. | Method and test kit for measuring concentration of a cleaning agent in a wash liquor |
| CN100567956C (en) * | 2005-11-30 | 2009-12-09 | 广东省农业科学院农业生物技术研究所 | A kind of lichee polysaccharide Determination on content method |
| CN101477038A (en) * | 2009-01-15 | 2009-07-08 | 宋秋兰 | Method for measuring ganoderma polyoses content in ganoderma products by sulfuric acid-phynol method |
-
2010
- 2010-02-05 CN CN 201010108638 patent/CN101776611B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495012A (en) * | 2011-12-05 | 2012-06-13 | 中国水产科学研究院黄海水产研究所 | Method for identifying sugar-doped dried sea cucumber |
| CN105628631A (en) * | 2015-12-28 | 2016-06-01 | 天津科技大学 | Rapid detection method of biological catalytic conversion rate of hydrocortisone |
| CN105628631B (en) * | 2015-12-28 | 2019-02-22 | 天津科技大学 | A kind of rapid detection method of hydrocortisone Biocatalytic Conversion rate |
| CN110174362A (en) * | 2019-05-05 | 2019-08-27 | 贵州中烟工业有限责任公司 | A method of detection neutral sugar and acid sugared content |
| CN110174361A (en) * | 2019-05-05 | 2019-08-27 | 贵州中烟工业有限责任公司 | A kind of measurement method of total reducing sugar |
| CN110174362B (en) * | 2019-05-05 | 2024-05-03 | 贵州中烟工业有限责任公司 | Method for detecting content of neutral sugar and acidic sugar |
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