CN202533367U - Endotoxin fungus detector - Google Patents
Endotoxin fungus detector Download PDFInfo
- Publication number
- CN202533367U CN202533367U CN2012201788371U CN201220178837U CN202533367U CN 202533367 U CN202533367 U CN 202533367U CN 2012201788371 U CN2012201788371 U CN 2012201788371U CN 201220178837 U CN201220178837 U CN 201220178837U CN 202533367 U CN202533367 U CN 202533367U
- Authority
- CN
- China
- Prior art keywords
- endotoxin
- test tube
- dash receiver
- output terminal
- chip microcomputer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The utility model discloses an endotoxin fungus detector which comprises a control circuit, a singlechip, a light emitting plate, a receiving plate and a constant temperature test tube rack, wherein the control circuit is communicated with the singlechip, the light emitting plate, the receiving plate and the constant temperature test tube rack. The endotoxin fungus detector has the advantages that a light source with two wave lengths is used as lighting light; and the endotoxin fungus detector simultaneously has two detection methods of turbidimetry and colorimetry, and the detection methods can be selected as required, so that the detection accuracy is guaranteed, the detection cost except high-precision detection is also considered, and the endotoxin fungus detector can be popularized and used in a large range.
Description
Technical field
The utility model relates to a kind of endotoxin fungi detector.
Background technology
Endotoxin is gramnegative bacterium growth or the dead toxicant that discharges, and is the important heating source of human body, can cause the human body heating, seriously can cause SIRS, pyemia, DIC, MODS.Clinically; Through endotoxin concns in the quantitative human body body fluid, judge the gradient of infection of human body, direct clinical work: the early diagnosis of gram positive bacterial infection disease; The quick auxiliary diagnosis of heating cause; Offer help for the clinician rationally screens suitable pharmaceutical fast, anti-infective therapy's effect and prognosis are judged in the anticipation of intestinal endotoxemia and assessment; Detect at present endotoxin as a kind of very effective cause of disease detection method, obtaining applying more and more widely.
Endotoxin detector (containing fungi, peptide glycan detection) based on optics adopts light transmission, and its principle is as shown in Figure 1.Light transmission is the basis with Lambert-Beer's law and light scattering theory, that is: the strength relationship of transmission light intensity and incident light that sees through certain concentration solution is following:
I=I
0e
-ε cdOr D=LnI
0/ I=ε cd
Wherein: I
0Represent to launch light intensity and transmitted light intensity respectively with I, c representes the concentration of certain material solution, and d representes that light passes the path of solution, and ε representes the absorption coefficient of light of certain material, and D is called absorbance.If path d is a constant, then solution concentration c is directly proportional with absorbance D.
Can know from Lambert-Beer's law and light scattering theory,, measure it and just can obtain the concentration of solution and the content of material through solution absorbency as long as select suitable wavelength light.So we only need measure the transmitted intensity that passes reagent bottle, just can measure the concentration of solution, and then detect endotoxin concns.
But present endotoxin detector on the market; All be endotoxin to be detected through turbidimetry; Turbidimetry is that the variation that produces concentration after adopting photometry according to endotoxin and reagent mix is taked a stable basic point as a reference; Read the time that reaches this RP, the accurate quantitative concentration that obtains sample with the anti-concentration that pushes away sample of typical curve equation contrast.The turbidimetry consumed time is long, and operability requires very strict, causes the endotoxic pollution of artificial property external source easily and causes distortion as a result.Can not reach the endotoxic purpose of effective detection.
The utility model content
In order to solve the existing coarse problem of endotoxin testing result, the utility model provides a kind of endotoxin fungi detector that can effectively improve the endotoxin accuracy of detection.
In order to realize above-mentioned technical purpose; The technical scheme of the utility model is; A kind of endotoxin fungi detector comprises control circuit, single-chip microcomputer, luminous plaque, dash receiver and constant temperature test tube rack, and described control circuit is communicated by letter with the constant temperature test tube rack with single-chip microcomputer, luminous plaque, dash receiver respectively and is connected.
Described a kind of endotoxin fungi detector; Described control circuit comprises light emitting control solid-state relay, amplifying circuit, simulation Port Multiplier circuit, analog to digital conversion circuit, temperature control solid-state relay and temperature sensor; The control end of described light emitting control solid-state relay is connected to single-chip microcomputer; The output terminal of light emitting control solid-state relay is connected to luminous plaque, and the input end of described amplifying circuit is connected to dash receiver, and the output terminal of amplifying circuit is connected to the input end of simulation Port Multiplier; The output terminal of described simulation Port Multiplier is connected to the input end of analog to digital conversion circuit; The output terminal of analog to digital conversion circuit is connected to single-chip microcomputer, and the control end of described temperature control solid-state relay is connected to single-chip microcomputer, and the output terminal of temperature control solid-state relay is connected to heating plate; Described temperature sensor is connected to heating plate with the collecting temperature data, and the output terminal of temperature sensor is connected to single-chip microcomputer.
Described a kind of endotoxin fungi detector; Described test tube rack comprises flat base, hole bar and electric heating sheets; Have the test tube hole that is used for fixing test tube on the bar of described hole, the hole bar is fixed in base one side, and described electric heating sheets is fixed in the base opposite side.
Described a kind of endotoxin fungi detector, described luminous plaque and dash receiver are individually fixed in the both sides of hole bar, have in a fixing side of dash receiver on the bar of described hole to be used for the slit that dash receiver receives light.
Described a kind of endotoxin fungi detector, described luminous plaque are provided with the red light source of 660nm wavelength and two kinds of light sources of blue-light source of 405nm wavelength.
Described a kind of endotoxin fungi detector, described dash receiver is provided with photoelectric sensor, and described photoelectric sensor communication link is connected to control circuit.
Described a kind of endotoxin fungi detector, the width of described slit is 0.5mm.
The technique effect of the utility model is; The light source that adopts two kinds of wavelength is as irradiates light, avoided since during single light source transillumination different colours solution transmittance differ and collection exerts an influence to data problem, and possess simultaneously than turbid and two kinds of detection methods of colorimetric; Can select detection method as required; Promptly guaranteed the accuracy that detects, also taken into account the detection cost when not doing high-precision detection and requiring simultaneously, be suitable for promoting the use of on a large scale.
Below in conjunction with accompanying drawing the utility model is described further.
Description of drawings
Fig. 1 is the detection schematic diagram of light transmission;
Fig. 2 is the structural representation of the utility model;
Fig. 3 is the circuit block diagram of the utility model;
Fig. 4 is the structural representation of the utility model constant temperature test tube rack;
Fig. 5 is the synoptic diagram that is used for the slit of light source reception on the utility model constant temperature test tube rack;
Fig. 6 is the substructure synoptic diagram of the utility model constant temperature test tube rack;
Fig. 7 is the side sectional view of the utility model constant temperature test tube rack;
Wherein 1 is base, and 2 is the hole bar, and 3 is electric heating sheets, and 4 is the test tube hole, and 5 is luminous plaque, and 6 is dash receiver, and 7 is slit.
Embodiment
Referring to Fig. 2, the utility model comprises control circuit, single-chip microcomputer, luminous plaque, dash receiver and constant temperature test tube rack, and control circuit is communicated by letter with the constant temperature test tube rack with single-chip microcomputer, luminous plaque, dash receiver respectively and is connected.
Referring to Fig. 3; Control circuit comprises light emitting control solid-state relay, amplifying circuit, simulation Port Multiplier circuit, analog to digital conversion circuit, temperature control solid-state relay and temperature sensor, and the control end of light emitting control solid-state relay is connected to single-chip microcomputer, and the output terminal of light emitting control solid-state relay is connected to luminous plaque; The input end of amplifying circuit is connected to dash receiver; The output terminal of amplifying circuit is connected to the input end of simulation Port Multiplier, and the output terminal of simulation Port Multiplier is connected to the input end of analog to digital conversion circuit, and the output terminal of analog to digital conversion circuit is connected to single-chip microcomputer; The control end of temperature control solid-state relay is connected to single-chip microcomputer; The output terminal of temperature control solid-state relay is connected to heating plate, and temperature sensor is connected to heating plate with the collecting temperature data, and the output terminal of temperature sensor is connected to single-chip microcomputer.
Referring to Fig. 4, Fig. 5, Fig. 6, Fig. 7, test tube rack comprises flat base 1, hole bar 2 and electric heating sheets 3, has the test tube hole 4 that is used for fixing test tube on the hole bar 2, and hole bar 2 is fixed in base 1 one sides, and electric heating sheets 3 is fixed in base 1 opposite side.Luminous plaque 5 and dash receiver 6 are individually fixed in the both sides of hole bar 2, have the slit 7 that is used for dash receiver 6 reception light in a fixing side of dash receiver 6 on the hole bar 2, and the width of slit 7 is 0.5mm.Luminous plaque 5 is provided with the red light source of 660nm wavelength and two kinds of light sources of blue-light source of 405nm wavelength, and light source adopts light emitting diode.Dash receiver 6 is provided with photoelectric sensor, and the photoelectric sensor that adopts in the present embodiment is a silicon photocell, and model is S1133, and the photoelectric sensor communication link is connected to control circuit.
The utility model adopts SCM system; Have that volume is little, in light weight, low in energy consumption, the reliability advantages of higher, adopted temperature control method preferably simultaneously, no matter environment temperature, heating plate power; Can both satisfy the temperature and the accuracy requirement of instrument; Overshoot phenomenon can not occur, a slit in the logical via bar of the light that luminous plaque sends on the constant temperature test tube rack shines on the silicon photocell of dash receiver, and the width of slit is 0.5mm.Each hole bar has 8 such test tube hole and slits, adopts Ultraprecision Machining, has guaranteed the consistance of each hole and slit.
The utility model comprises four big functional modules: typical curve, detection overview, historical record and instrument setting, and its concrete effect is:
Typical curve: this module comprises two functions, and the internal memory curve reads and the new work typical curve.(1) the internal memory curve reads, and main effect is the typical curve that reads in the calculator memory, then through the reaction time comparison typical curve of test sample, draws the endotoxin concns of test sample.The reaction time here is meant that the penetrability of reaction mixture arrives the time of preset transparency value (R0).(2) major function of new work typical curve is through choice criteria endotoxin series solution, carries out the detection reaction time like 1.0EU/ml, 0.5E/ml, 0.25EU/ml and 0.125EU/ml, formulates typical curve, and typical curve is saved in card or internal memory.
Detect overview: detect overview and mainly comprise three partial functions, detection curve, printing detection information and detection information are preserved.(1) major function of detection curve is to show testing result, reaction time and will detect data to be depicted as curve display and to come out.(2) print detection information, main effect is output and prints testing result information.(3) detection information is preserved, and major function is that testing result is saved in database, and the moment that needs can inquire about, demonstration and printout.
Historical record: this module comprises an even partial function, prints the detection information and the recovery.(1) the main effect of printing detection information is, the user can inquire the object information that detects recently through query function, and with its printout.(2) recovery is calculated, through the recovery negative, that positive control calculates this group detectable.
Instrument is provided with: instrument is provided with and mainly comprises five partial functions: parameter setting, temperature adjustment, penetrability, optical density consistance and test tube setting.(1) effect is set is the setting that detection system is had important relevant Several Parameters to parameter, mainly contains the preset OD restriction of acquiescence, acquiescence time pulverised phase, test tube quantity, COM1, conclusion determination methods and filtering algorithm.(2) the temperature corrective action is that the detected temperatures of detection system is calibrated, and the normal detected temperatures scope of system is 36.9 degree～37.1 degree.(3) mainly to act on be the way luminosity data that shows that directly detection system is returned to penetrability.(4) mainly to act on be show one group of test tube testing result one straightforward to the optical density consistance.(5) the main effect of test tube setting is system to be set carry out endotoxin or fungi detection, the extension rate of reagent and the selected typical curve of each test tube.
Claims (7)
1. an endotoxin fungi detector is characterized in that, comprises control circuit, single-chip microcomputer, luminous plaque, dash receiver and constant temperature test tube rack, and described control circuit is communicated by letter with the constant temperature test tube rack with single-chip microcomputer, luminous plaque, dash receiver respectively and is connected.
2. a kind of endotoxin fungi detector according to claim 1; It is characterized in that; Described control circuit comprises light emitting control solid-state relay, amplifying circuit, simulation Port Multiplier circuit, analog to digital conversion circuit, temperature control solid-state relay and temperature sensor, and the control end of described light emitting control solid-state relay is connected to single-chip microcomputer, and the output terminal of light emitting control solid-state relay is connected to luminous plaque; The input end of described amplifying circuit is connected to dash receiver; The output terminal of amplifying circuit is connected to the input end of simulation Port Multiplier, and the output terminal of described simulation Port Multiplier is connected to the input end of analog to digital conversion circuit, and the output terminal of analog to digital conversion circuit is connected to single-chip microcomputer; The control end of described temperature control solid-state relay is connected to single-chip microcomputer; The output terminal of temperature control solid-state relay is connected to heating plate, and described temperature sensor is connected to heating plate with the collecting temperature data, and the output terminal of temperature sensor is connected to single-chip microcomputer.
3. a kind of endotoxin fungi detector according to claim 1; It is characterized in that; Described test tube rack comprises flat base, hole bar and electric heating sheets; Have the test tube hole that is used for fixing test tube on the bar of described hole, the hole bar is fixed in base one side, and described electric heating sheets is fixed in the base opposite side.
4. a kind of endotoxin fungi detector according to claim 1 is characterized in that described luminous plaque and dash receiver are individually fixed in the both sides of hole bar, has in a fixing side of dash receiver on the bar of described hole to be used for the slit that dash receiver receives light.
5. a kind of endotoxin fungi detector according to claim 1 is characterized in that, described luminous plaque is provided with the red light source of 660nm wavelength and two kinds of light sources of blue-light source of 405nm wavelength.
6. a kind of endotoxin fungi detector according to claim 1 is characterized in that described dash receiver is provided with photoelectric sensor, and described photoelectric sensor communication link is connected to control circuit.
7. a kind of endotoxin fungi detector according to claim 4 is characterized in that the width of described slit is 0.5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012201788371U CN202533367U (en) | 2012-04-24 | 2012-04-24 | Endotoxin fungus detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012201788371U CN202533367U (en) | 2012-04-24 | 2012-04-24 | Endotoxin fungus detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202533367U true CN202533367U (en) | 2012-11-14 |
Family
ID=47134543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012201788371U Expired - Fee Related CN202533367U (en) | 2012-04-24 | 2012-04-24 | Endotoxin fungus detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202533367U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102650593A (en) * | 2012-04-24 | 2012-08-29 | 湖南迪斯生物技术有限公司 | Endotoxin fungus detector |
| CN106226525A (en) * | 2016-07-18 | 2016-12-14 | 丁卫东 | A kind of limulus test endotoxin fungal detection instrument |
| CN106680246A (en) * | 2016-12-31 | 2017-05-17 | 北京科兴中维生物技术有限公司 | Device for detecting flocculation units of toxoid and application of device |
-
2012
- 2012-04-24 CN CN2012201788371U patent/CN202533367U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102650593A (en) * | 2012-04-24 | 2012-08-29 | 湖南迪斯生物技术有限公司 | Endotoxin fungus detector |
| CN106226525A (en) * | 2016-07-18 | 2016-12-14 | 丁卫东 | A kind of limulus test endotoxin fungal detection instrument |
| CN106680246A (en) * | 2016-12-31 | 2017-05-17 | 北京科兴中维生物技术有限公司 | Device for detecting flocculation units of toxoid and application of device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103399006B (en) | Color RGB (red, green and blue)-component-based urine analysis device and processing method thereof | |
| CN107449749A (en) | Water quality detection equipment and its water quality detection system | |
| CN105548128A (en) | Method and device for detecting chlorophyll of coastal zone water body in situ through double optical path method | |
| CN202533367U (en) | Endotoxin fungus detector | |
| CN104568946A (en) | Intelligent optical fiber pH sensor | |
| CN203949870U (en) | A kind of liquid physical and chemical parameter measurement mechanism based on fluorescence analysis | |
| CN104931441A (en) | Method and device for quickly detecting hemoglobin | |
| CN105424692B (en) | Urine desiccation analytical equipment and analysis method based on more monochromatic light and optical fiber | |
| CN102650593A (en) | Endotoxin fungus detector | |
| CN1595105A (en) | Integrated minisize optical analyser | |
| CN203310744U (en) | Liquid core waveguide food detector | |
| CN202204751U (en) | Detector for fast measuring liquid specific gravity, turbidity and color | |
| CN209542439U (en) | High-concentrated ozone analyzer | |
| CN202033293U (en) | Chloramphenicol concentration quantitative detection device based on test strip | |
| CN208721577U (en) | A kind of device by light-intensity test liquid sugar content | |
| CN102042981B (en) | In-situ tester of dissolved inorganic carbon | |
| CN207366368U (en) | A kind of fruit quality comprehensive detection analyzer | |
| CN203606362U (en) | Hardware system of immunofluorescence detector | |
| CN204882371U (en) | Digital photoelectricity biosensor | |
| CN110261356A (en) | A kind of online dissolved oxygen sensing system based on Fluorimetric Quenching Method | |
| CN103792217A (en) | Coffee concentration detection system and method based on light-emitting diode (LED) induced fluorescence spectrum | |
| CN201532361U (en) | Multifunctional agricultural product safety rapid detecting instrument | |
| CN204177810U (en) | Plasma proteins quick analysis system | |
| CN205404410U (en) | Double -light -path method littoral zone water chlorophyll normal position monitoring devices | |
| CN204439543U (en) | The main component content measuring system of a kind of emulsion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121114 Termination date: 20170424 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |