CN202421174U - Isolated heart perfusion device - Google Patents
Isolated heart perfusion device Download PDFInfo
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- CN202421174U CN202421174U CN2012200297512U CN201220029751U CN202421174U CN 202421174 U CN202421174 U CN 202421174U CN 2012200297512 U CN2012200297512 U CN 2012200297512U CN 201220029751 U CN201220029751 U CN 201220029751U CN 202421174 U CN202421174 U CN 202421174U
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Abstract
The utility model discloses an isolated heart perfusion device, which belongs to the field of medical instruments. The isolated heart perfusion device comprises a bracket mounted on a bottom plate, wherein a perfusion heat insulating groove and a heart heat insulating groove are mounted on the bracket; three perfusion columns are arranged in the perfusion heat insulating groove; a hot water inlet and a hot water outlet are formed at the side wall of the groove; scales are engraved on the external sides of the perfusion columns; a rubber plug with a liquid inlet pipe covers a top opening; a liquid outlet at the bottom is communicated with three three-way valves below the perfusion heat insulating groove; the three three-way valves are connected with a three-way heart perfusion pipe; a heart heat insulating chamber is formed in the heart heat insulating groove; the upper end surface of the heart heat insulating groove is provided with a concave upper heat insulating plate which can be opened and closed and is provided with an overflow hole at the central position; and the lower end surface of the heart heat insulating groove is provided with a concave lower heat insulating plate which can rotate and is provided with a hole in the middle. The isolated heart perfusion device disclosed by the utility model has the advantages of small volume, compact structure and convenience in operation; by using a perfusion heart, a high number of myocardial cells with high qualities can be obtained; and the obtained myocardial cells can be used for carrying out electrophysiological experiments, transgenic experiments, cell culture, molecular biology experiment and the like.
Description
Technical field
The utility model relates to a kind of isolated heart perfusion device, belongs to the Medical Instruments technical field.Be mainly used in influence and the separating myocardium cell of research medicine to the isolated heart function.
Background technology
Along with progress of science and technology with to the continuous development of cardiac studies, the cardiac muscle cell has become the important tool of research myocardial structural, metabolism, function, pathologic, physiologic, its mechanism of falling ill and cardiomyopathies treatment.At present, great majority research all adopts Langendorff device biology enzyme perfusion digestion method to separate ripe cardiac muscle cell.Langendorff isolated heart perfusion device comprises several coiled pipes, glass container and connecting tube, mainly relies on gravity to carry out perfusion, and it about 1 m, can not be put into and realize sterile working in the common super-clean bench highly usually.This device is because the pipeline of liquid process is oversize simultaneously, and that the perfusion bottle causes too greatly is time-consuming, waste liquid, logical oxygen difficulty, bubble are difficult for discharging.
Summary of the invention
The purpose of the utility model mainly is to solve the said apparatus volume can not put into the super-clean bench sterile working greatly; Logical oxygen difficulty; The defective that bubble be difficult for to be discharged, provide a kind of have simple and practical, the isolated heart perfusion device of the high and advantages such as quality is good, low price of cardiac muscle cell's output capacity.
The utility model is realized through following technical scheme: a kind of isolated heart perfusion device; It comprises perfusion insulation groove, heart insulation groove, threeway cardiac perfusion pipe, base plate and support five parts; Support is installed on the base plate, and perfusion insulation groove and heart insulation groove are fixed on the support up and down successively and can move up and down along support; In described perfusion insulation groove, three perfusion posts are arranged, hot inlet opening and hot apopore are arranged on the groove sidewall; Scale is carved with in the perfusion post outside, is stamped the rubber stopper of bringing the liquid pipe in the open top, and the liquid outlet of bottom is connected with three T-valve that are positioned at perfusion insulation groove below; Three T-valve link to each other with threeway cardiac perfusion pipe through woven hose; In the described heart insulation groove heart moist closet is arranged, the upper surface of heart insulation groove have can folding and centre bit be equipped with thermal insulative board on the matrix in perfusion hole, there is thermal insulative board under the matrix with holes of the centre that can rotate the lower surface; The semicircle matrix plates that described upward thermal insulative board is installed the ability switches by two symmetries are formed, and there is semi-circular hole its center, and middle formation one is passed through with the perfusion hole confession threeway cardiac perfusion pipe lower end that threeway cardiac perfusion pipe cooperates when two blocks of semicircle matrix plates are closed.
Three T-valve link to each other with threeway cardiac perfusion pipe through woven hose and can control three kinds of perfusates respectively and carry out perfusion, simultaneously can single-pass enzyme liquid, and avoid enzyme liquid diluted.The perfusion post has three effects that can study three kinds of different drug to isolated heart simultaneously.
Last thermal insulative board on the heart insulation groove forms an airtight keeping warmth space with following thermal insulative board makes heart moist closet temperature stabilization.Hole under the heart insulation groove on the thermal insulative board can be used to collect perfusate.There is hot water to enter the hole on the heart insulation groove lateral wall and hot water portals.The water of water bath with thermostatic control pump arrives perfusion insulation groove through constant temperature water pipe through heart insulation groove again, gets back to the water bath with thermostatic control pump at last, thereby guarantees to place the steady temperature and the humidity of isolated heart in the heart insulation groove, guarantees the steady temperature of perfusate in the perfusion post.During experiment, constant flow pump and pressure controller inject perfusate in the perfusion groove through rubber stopper constant voltage or constant current, get into heart through T-valve, threeway cardiac perfusion pipe then and carry out perfusion.
Have following characteristics:
1) little, the compact conformation, easy to operate of this device volume can place and accomplish whole perfusion in the super-clean bench.Thereby guarantee isolated cell under aseptic condition, the cardiac muscle cell that separation obtains can cultivate and carry out cytologic experiment.
2) this device perfusion column volume is little, under the prerequisite that does not influence separating effect, helps the clostridiopetidase A of conserve expensive and the consumption of proteinase, reduces experimental cost.
3) this device can combine constant flow pump or pressure controller to select constant current or constant pressure infusing heart according to the experiment needs.
4) can carry out multiple drug perfusion experiment, study of the effect of three kinds of different drug simultaneously isolated heart.
5) the sustainer sleeve pipe can be changed according to the animal used as test difference, thereby this device can be used for the isolated heart perfusion of common experimental animals such as rat, mouse, rabbit.
The beneficial effect of the utility model is: volume is little, compact conformation, easy to operate; Utilize this device perfusion heart can obtain high quantity and high-quality cardiac muscle cell, the cardiac muscle cell of acquisition can carry out electric Physiological Experiment, transgenic experiments; Cellular incubation, molecular biology experiment etc.
Description of drawings
Accompanying drawing 1 is the structural representation of the utility model.
Among the figure: 1, base plate, 2, metal support, 3, perfusion insulation groove, 4, the perfusion post; 5 skin plugs, 6, perfusion insulation groove hot water portals, 7, perfusion insulation groove hot water enters the hole, 8, T type T-valve; 8, threeway cardiac perfusion pipe, 10, heart insulation groove, 11, heart insulation groove upper cap, 12, heart insulation groove lower cover; 13, heart moist closet, 14, heart insulation groove hot water portals, 15, heart insulation groove hot water enters the hole, 16, the perfusion hole.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further specified.
Shown in accompanying drawing; It has perfusion insulation groove 3, heart insulation groove 10, threeway cardiac perfusion pipe 9, base plate 1 and support 2 five parts a kind of isolated heart perfusion device; Support 2 is vertically mounted on the base plate 1, and perfusion insulation groove 3 is fixed on the support 2 up and down successively with heart insulation groove 10 and can moves up and down along support; In described perfusion insulation groove 3, three perfusion posts 4 are arranged, hot inlet opening 7 and hot apopore 6 are arranged on the groove sidewall; Scale is carved with in the perfusion post outside, is stamped the rubber stopper 5 of bringing the liquid pipe in the open top, and the liquid outlet of bottom is connected with three T-valve 8 that are positioned at perfusion insulation groove 3 belows; Three T-valve 8 link to each other with threeway cardiac perfusion pipe 9 through woven hose; In the described heart insulation groove 10 heart moist closet 13 is arranged, the upper surface of heart insulation groove 10 have can folding and centre bit be equipped with thermal insulative board 11 on the matrix in perfusion hole 16, there is thermal insulative board 12 under the matrix with holes of the centre that can rotate the lower surface; On heart insulation groove sidewall, there is hot water to enter hole 15 and portals 14 with hot water.
The described thermal insulative board 11 of going up is made up of the semicircle matrix plate of two symmetry installation ability switches; There is semi-circular hole its center, and middle formation one supplies threeway cardiac perfusion pipe lower ends to pass through with the perfusion hole 16 that threeway cardiac perfusion pipe 9 cooperates when two blocks of semicircle matrix plates are closed.
The sustainer sleeve pipe of threeway cardiac perfusion pipe lower end can be according to the needs of animal used as test size, change a social system with the injection needle of different model to form.Cut the sharp-pointed part of syringe needle front end, remaining front end polished smooth, and in otch upper end 2 millimeters places frustrate an indenture so that with the aortic root ligation to the sustainer sleeve pipe.
Use the specific operation process of the utility model separating myocardium cell following:
1) opens the thermostatical circulating water pump, let thermostatted water circulate, make the temperature of liquid in perfusion insulation groove, the heart insulation groove reach 40 ℃;
2) open the oxygen channel valve, regulate the pressure size according to the experiment needs bubble is evenly entered in tyrode, the enzyme liquid continuously lessly;
3) with the top of rubber stopper jam-pack perfusion post, open when peristaltic pump is irritated to the perfusion post first scale with tyrode and stop to pour into, open threeway, when liquid in the perfusion post arrived certain altitude, the sustainer sleeve pipe is the flow liquid body no longer;
4) the suspension heart is on the sustainer sleeve pipe, and jacket insertion depth must not surpass aorta petal, and toe-in is pricked fixing.Open peristaltic pump through the retrograde perfusion tyrode of sustainer, after heart recovers to beat, move heart insulation groove heart is wrapped in the heart moist closet fully, close thermal insulative board up and down.Change enzyme liquid circulation perfusion 20-30 min behind the 5min,, collect perfusate and make its recycle at heart insulation groove held beaker.
5, become soft when heart,, cut off atrium and connective tissue inserting under the cardiac scissors in the KB liquid; Ventricular organization is cut into about 1mm * 1mm * 1mm fragment, blows and beats gently with thick opening suction pipe, just visible individual cells and cell mass come off; Use 200 purpose nylon net filters then, filtrate and under room temperature, leave standstill 20min, let the cell natural subsidence; Abandon supernatant, add KB liquid natural subsidence again, 3 times repeatedly.
Each the rat heart cell yield that utilizes this device to separate can reach 4-6 * 10
6Cell number, the shaft-like rate of cell is average about 75%, and the elongated rod shape cardiac muscle cell more than 90% stretches and unfolds, and cell does not have obviously beats, and presents band clearly, and edges at two ends is sharp keen.Therefore utilize this device can obtain high yield and high-quality rat myocardial cell, and can reduce experimental cost, simple economy is worth applying in the cardiovascular research field.
Claims (2)
1. isolated heart perfusion device is characterized in that: it comprises perfusion insulation groove (3), heart insulation groove (10), threeway cardiac perfusion pipe (9), base plate (1) and support (2); Support (2) is installed on the base plate (1), and perfusion insulation groove (3) and heart insulation groove (10) are fixed on support (2) up and down successively and go up and can move up and down along support; In described perfusion insulation groove (3), three perfusion posts (4) are arranged, hot inlet opening (7) and hot apopore (6) are arranged on the groove sidewall; Scale is carved with in the perfusion post outside, is stamped the rubber stopper (5) of bringing the liquid pipe in the open top, and the liquid outlet of bottom is connected with three T-valve (8) that are positioned at perfusion insulation groove (3) below; Three T-valve (8) link to each other with threeway cardiac perfusion pipe (9) through woven hose; In the described heart insulation groove (10) heart moist closet (13) is arranged; The upper surface of heart insulation groove (10) have can folding and centre bit be equipped with thermal insulative board (11) on the matrix of perfusion hole (16), there is thermal insulative board (12) under the matrix with holes of the centre that can rotate the lower surface; On heart insulation groove sidewall, there is hot water to advance hole (15) and hot water portal (14).
2. isolated heart perfusion device according to claim 1; It is characterized in that: the described thermal insulative board (11) of going up is made up of the semicircle matrix plate of two symmetry installation ability switches; There is semi-circular hole its center, and middle formation one supplies threeway cardiac perfusion pipe lower end to pass through with the perfusion hole (16) that threeway cardiac perfusion pipe (9) cooperates when two blocks of semicircle matrix plates are closed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200297512U CN202421174U (en) | 2012-01-30 | 2012-01-30 | Isolated heart perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200297512U CN202421174U (en) | 2012-01-30 | 2012-01-30 | Isolated heart perfusion device |
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| Publication Number | Publication Date |
|---|---|
| CN202421174U true CN202421174U (en) | 2012-09-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012200297512U Expired - Fee Related CN202421174U (en) | 2012-01-30 | 2012-01-30 | Isolated heart perfusion device |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104897886A (en) * | 2015-05-27 | 2015-09-09 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN106940333A (en) * | 2017-04-17 | 2017-07-11 | 山西医科大学 | The outer Electrophysiology research perfusion system of isolated cells |
| CN108535463A (en) * | 2018-05-07 | 2018-09-14 | 厦门大学附属第医院 | Perfused isolated heart experimental provision and Perfused isolated heart experimental method |
| CN109430253A (en) * | 2019-01-08 | 2019-03-08 | 成都泰盟软件有限公司 | A kind of Perfused isolated heart system that binary channels holocentric is dirty |
| CN109526944A (en) * | 2019-01-07 | 2019-03-29 | 成都泰盟软件有限公司 | A kind of exhaust bubble structure in Perfused isolated heart system |
| CN111657268A (en) * | 2020-03-31 | 2020-09-15 | 同济大学 | Small animal heart imaging perfusion hose system |
| CN111849749A (en) * | 2020-03-31 | 2020-10-30 | 同济大学 | High-efficient piece-rate system of adult toy cardiac muscle cell |
-
2012
- 2012-01-30 CN CN2012200297512U patent/CN202421174U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104897886A (en) * | 2015-05-27 | 2015-09-09 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN104897886B (en) * | 2015-05-27 | 2017-02-22 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN106940333A (en) * | 2017-04-17 | 2017-07-11 | 山西医科大学 | The outer Electrophysiology research perfusion system of isolated cells |
| CN108535463A (en) * | 2018-05-07 | 2018-09-14 | 厦门大学附属第医院 | Perfused isolated heart experimental provision and Perfused isolated heart experimental method |
| CN108535463B (en) * | 2018-05-07 | 2023-11-10 | 厦门大学附属第一医院(厦门市第一医院、厦门市红十字会医院、厦门市糖尿病研究所) | Isolated heart perfusion experimental device and isolated heart perfusion experimental method |
| CN109526944A (en) * | 2019-01-07 | 2019-03-29 | 成都泰盟软件有限公司 | A kind of exhaust bubble structure in Perfused isolated heart system |
| CN109430253A (en) * | 2019-01-08 | 2019-03-08 | 成都泰盟软件有限公司 | A kind of Perfused isolated heart system that binary channels holocentric is dirty |
| CN111657268A (en) * | 2020-03-31 | 2020-09-15 | 同济大学 | Small animal heart imaging perfusion hose system |
| CN111849749A (en) * | 2020-03-31 | 2020-10-30 | 同济大学 | High-efficient piece-rate system of adult toy cardiac muscle cell |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120905 Termination date: 20210130 |