CN202231776U - Microsection scanning device - Google Patents
Microsection scanning device Download PDFInfo
- Publication number
- CN202231776U CN202231776U CN2011203825525U CN201120382552U CN202231776U CN 202231776 U CN202231776 U CN 202231776U CN 2011203825525 U CN2011203825525 U CN 2011203825525U CN 201120382552 U CN201120382552 U CN 201120382552U CN 202231776 U CN202231776 U CN 202231776U
- Authority
- CN
- China
- Prior art keywords
- imaging system
- section
- automatic
- mobile platform
- dimensional mobile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Image Input (AREA)
Abstract
The utility model relates to a microsection scanning device which comprises a computer, an automatic three-dimensional mobile platform, a light source, a section supporting frame and an imaging system, wherein the light source, the section supporting frame and the imaging system are sequentially arranged coaxially; the automatic three-dimensional mobile platform is connected with the section supporting frame or imaging system, and is controlled by a controller to drive the section or imaging system to move in three-dimensional directions; the controller and the imaging system are respectively electrically connected with the computer; the section to be detected is arranged on the automatic three-dimensional mobile platform or section supporting frame; the imaging system comprises an objective, an imaging lens and a planar array image sensor which are sequentially arranged coaxially; and an illuminating system comprises the light source and illuminating lenses. The utility model has the following advantages: the section makes a continuous motion relative to the imaging system in the scanning process, and thus, the speed is high; the control accuracy requirement is low, thereby lowering the cost; and the common planar array image sensor can be directly used for establishing a focusing model without any additional auxiliary focusing device, thereby further lowering the cost.
Description
Technical field
The utility model belongs to the microscopy field, is specifically related to a kind of scanning means of microsection.
Background technology
Virtual microscopic obtains high-resolution image when can obtain big visual field, is widely used, and mainly contain three kinds of technology at present: first kind is; Array image sensor cooperates the scanning of section, specifically is after a visual field is moved in section, stops and waits array image sensor to make public; Move to next visual field behind the end exposure again; The rest may be inferred, obtains the view data of a plurality of continuous visual fields, and the image of visual field is helped in splicing at last.This method has and realizes simply, but section need loiter, and also need wait for a period of time after stopping again, and guarantee that section begins to make public after stable again, thereby speed is slow; Second kind is, line scan image sensor cooperates the uniform motion of section, is commonly referred to as line and sweeps mode, and it is when scanning; Section is the motion that remains a constant speed, and speed is very fast, but control is complicated; Require the control precision of objective table high, and need auxiliary focusing mechanism, system cost is high; The third is, the scanning of microlens array mating surface array image sensor, and speed is very fast; But, focusing range focuses on because being tissue part to whole section; Picture quality is not fine yet, and its array image sensor needs customization, and system configuration is very complicated.
The utility model content
The technical problem that the utility model will solve is: overcome the deficiency of prior art, provide that a kind of sweep speed is fast, the scanning means of the simple microsection of system.
The utility model solves the technical scheme that its technical problem adopted: a kind of scanning means of microsection; Comprise computer; It is characterized in that: the illuminator, the imaging system that also comprise coaxial setting; Between said illuminator and the imaging system, also be provided with an automatic three-dimensional mobile platform; One controller is controlled said automatic three-dimensional mobile platform motion, can place section to be measured on the said automatic three-dimensional mobile platform; Said computer and said controller, imaging system electrically connect respectively each other, control said imaging system and control said automatic three-dimensional mobile platform synchronous working through said controller; And make the section to be measured and the imaging system of placing on the said automatic three-dimensional mobile platform be in the continuous relative motion.
Preferably, on the said automatic three-dimensional mobile platform, between said automatic three-dimensional mobile platform and the imaging system, be provided with the section bracing frame; Said section bracing frame is used to place section to be measured.
Preferably, said controller is controlled automatic three-dimensional mobile platform and is driven the section bracing frame and go up section to be measured, at the same time or separately directions X promptly laterally, the Y direction promptly vertically and the Z direction be axial motion.
Preferably, described imaging system comprises imaging lens and the imageing sensor that coaxial order is provided with.
Preferably, described imaging lens comprises object lens and the imaging len that coaxial order is provided with.
Preferably, said imageing sensor is an array image sensor; Described array image sensor, its minimum exposure time are the μ s order of magnitude.
Preferably, described illuminator comprises light source and illuminating lens.
A kind of scanning means of microsection comprises computer, it is characterized in that: also comprise illuminator, section bracing frame, imaging system that coaxial successively order is provided with, said section bracing frame is fixed, can place section to be measured on it; Said imaging system is connected with an automatic three-dimensional mobile platform; Said automatic three-dimensional mobile platform is controlled by a controller, can drive said imaging system motion; Said computer electrically connects with said controller, imaging system respectively each other, controls said automatic three-dimensional mobile platform synchronous working by the said imaging system of said computer control and through said controller; And make the section to be measured of placing on said imaging system and the said section bracing frame be in the continuous relative motion.
Preferably, said controller is controlled automatic three-dimensional mobile platform, drives said imaging system, at the same time or separately directions X promptly laterally, the Y direction promptly vertically and the Z direction be axial motion.
Preferably, described imaging system comprises imaging lens and the imageing sensor that coaxial order is provided with.
Preferably, described imaging lens comprises object lens and the imaging len that coaxial order is provided with.
Preferably, said imageing sensor is an array image sensor; Described array image sensor, its minimum exposure time are the μ s order of magnitude.
Preferably, described illuminator comprises light source and illuminating lens.
Compared with prior art, the beneficial effect of the utility model is: 1) scan certain band constantly, section is continuous motion, does not have the time of staying, and sweep speed is able to effective raising; 2) section does not need strict uniform motion, and is lower to the control precision requirement of automatic carrier, realizes than being easier to, and reduces the cost of system yet; 3) adopt common array image sensor, need not use expensive line scan image sensor, further reduce system cost; 4) adopt common array image sensor, can directly set up the focusing model, do not need extra auxiliary focusing device with it.
Description of drawings
Fig. 1 is the structured flowchart of the utility model embodiment one;
Fig. 2 is the structured flowchart of the utility model embodiment two;
Fig. 3 is section is divided into M scanning band along the Y direction a sketch map;
Fig. 4 is that arbitrary scanning band is divided into N the sketch map of visual field continuously among Fig. 3;
Be labeled as among the figure: 1, illuminator; 11, light source; 12, illuminating lens; 2, automatic three-dimensional travelling carriage; 3, section bracing frame; 4, section; 5, imaging system; 51, object lens; 52, imaging len; 53, array image sensor; 6, computer; 7, controller.
Embodiment
Below in conjunction with accompanying drawing embodiment, the utility model done further describing:
Embodiment one
Like Fig. 1,3, shown in 4, a kind of scanning means of implementing above-mentioned scan method comprises computer 6, also comprises illuminator 1, the imaging system 5 of coaxial setting, between said illuminator 1 and the imaging system 5, also is provided with an automatic three-dimensional mobile platform 2; On the said automatic three-dimensional mobile platform 2, between said automatic three-dimensional mobile platform 2 and the imaging system 5, be provided with the section bracing frame; Said section bracing frame is used to place section to be measured;
Said automatic three-dimensional mobile platform 2 motions of one controller, 7 controls can be placed section to be measured 4 on the said automatic three-dimensional mobile platform 2; Said computer 6 and said controller 7, imaging system 5 electrically connect respectively each other, control said imaging system 5 and control said automatic three-dimensional mobile platform 2 synchronous workings through said controller 7; And make the section to be measured 4 of placing on the said automatic three-dimensional mobile platform 2 be in the continuous relative motion with imaging system 5;
The automatic three-dimensional mobile platform 2 of said controller 7 controls drives section bracing frames 3 and goes up sections 4 to be measured, at the same time or separately directions X promptly laterally, the Y direction promptly vertically and the Z direction be axial motion; Described imaging system 5 comprises imaging lens and the imageing sensor that coaxial order is provided with; Described imaging lens comprises the object lens 51 and imaging len 52 that coaxial order is provided with; Said imageing sensor is an array image sensor 53, and its minimum exposure time is the μ s order of magnitude; Described illuminator 1 comprises light source 11 and illuminating lens 12.
Embodiment two
Like Fig. 2,3, shown in 4, a kind of scanning means of microsection comprises computer 6, also comprises illuminator 1, section bracing frame 3, imaging system 5 that coaxial successively order is provided with, and said section bracing frame 3 is fixed, can place section to be measured 4 on it; Said imaging system 5 is connected with an automatic three-dimensional mobile platform 2; Said automatic three-dimensional mobile platform 2 is by a controller 7 control, and the automatic three-dimensional mobile platform 2 of said controller 7 controls drives said imaging system 5, at the same time or separately directions X promptly laterally, the Y direction promptly vertically and the Z direction be axial motion; Said computer 6 electrically connects with said controller 7, imaging system 5 respectively each other, controls said automatic three-dimensional mobile platform 2 synchronous workings by the said computer 6 said imaging systems 5 of control and through said controller 7; And make the section to be measured 4 of placing on said imaging system 5 and the said section bracing frame 3 be in the continuous relative motion;
Described imaging system 5 comprises imaging lens and the imageing sensor that coaxial order is provided with; Described imaging lens comprises the object lens 51 and imaging len 52 that coaxial order is provided with; Said imageing sensor is an array image sensor 53, and its minimum exposure time is the μ s order of magnitude; Described illuminator 1 comprises light source 11 and illuminating lens 12.
Described light source 11; Stronger brightness is provided; To guarantee that array image sensor 53 is operated under the minimum exposure time situation, can obtain the image of enough brightness, perhaps select powerful Halogen lamp LED (50W), LED (3W) or the like for use; Perhaps select light source for use, the i.e. flash of light of only between exposure period, working with synchronization flash function; Array image sensor 53 is to adopt progressive scan mode 4 to be carried out to picture to cutting into slices; At first need to cut into slices 4 along vertically being divided into several continuous scanning bands of M; The width of each scanning band is made as the size of the corresponding true field of array image sensor 53 at longitudinal direction; And each scanning band transversely is divided into several continuous scanning field of view again, and the length of each scanning field of view is made as the size of the corresponding true field of array image sensor 53 at horizontal direction.
As shown in Figure 3; It is section 4 is divided into M scanning band along the Y direction sketch map; Suppose that section 4 is along the directions X uniform motion; It is long to be Sx; Wide is Sy; The true fields that array image sensor 53 is corresponding are the long Fx that are, wide be the rectangle of Fy, and it is Sx that the scanning area of section 4 is divided into the individual continuous length of M; Wide is the scanning band of Fy, wherein: bracket function in
expression.
As shown in Figure 4; Be that wherein a scanning band is divided into N the sketch map of visual field continuously; Width is Fy; Length is that the scanning band of Sx is that unit is divided into N continuous visual field at directions X with the long Fx of true field, wherein:
The utility model has adopted will cut into slices 4 along vertically being divided into several continuous scanning bands; The width of each scanning band is made as the size of the corresponding true field of array image sensor 53 at longitudinal direction; And each scanning band transversely is divided into several continuous scanning field of view again; The length of each scanning field of view is made as the size of the corresponding true field of array image sensor 53 at horizontal direction, and each scanning band of control section 4 passes the true field of array image sensor 53 correspondences successively during work, and whenever passes a scanning field of view; Array image sensor 53 just makes public once; And the time for exposure is enough short, guarantees image blurring can the ignoring that relative motion causes, like this in scanning process; Section 4 is always in relative motion with array image sensor 53, has effectively improved sweep speed.
The motion of described section 4 refers to the relative motion of section 4 and array image sensor 53 and object lens 51, imaging len 52; Both can be section 4 motions, and array image sensor 53 and object lens 51, imaging len 52 be static, also can be that section 4 is static, array image sensor 53 and object lens 51, imaging len 52 motions.
The motion of said section 4 can be a uniform motion, can be non-uniform movement also, as long as the speed in guaranteeing between array image sensor 53 exposure periods can not cause image blurring getting final product.
The operation principle and the course of work of the utility model are following:
Like Fig. 1,2,3, shown in 4; Earlier section 4 to be measured vertically is divided into several continuous scanning bands along it; The width of each scanning band is made as the size of the true field of imaging system 5 at longitudinal direction; And each scanning band transversely is divided into several continuous scanning field of view again, and the length of each scanning field of view is made as the size of the true field of imaging system 5 at horizontal direction; Also comprise the steps:
A) control section 4 and imaging system 5 continuous motions relatively in the horizontal, that is, make the current scanning band of section 4 continue to pass through the true field of imaging system 5;
B) when the current scanning field of view of section 4 current scanning band moved the true field that gets into imaging system 5 fully, imaging system 5 began exposure, and the time for exposure is 1～80 μ s; This moment cut into slices 4 with imaging system 5 still in relative motion;
C) the exposed images data of the current scanning field of view of the current scanning band of collection;
The view data of the current scanning field of view that d) computer 6 will this current scan stripes band and all of current scanning band before storing image data merge, as the image data storage of current scanning band in the memory of computer 6;
E) computer 6 judges whether the current scanning band of section 4 scans completion; If do not accomplish, then the next scanning field of view of current scanning band regarded as current scanning field of view, and return step b); Otherwise, continue next step;
F) computer 6 merges the storing image data of all scanning bands of the view data of current scanning band and having scanned before, as the image data storage of these 4 whole scanning areas of cutting into slices in the memory of computer 6;
G) computer 6 judges whether all scanning bands of section 4 scan completion; If accomplish, control section 4 relatively moves with imaging system 5 in the vertical, and imaging system 5 is positioned on the next one scanning band of section 4, and first scanning field of view that the next one scans band is regarded as current scanning field of view, and returns step a); Otherwise, finish scanning to this section 4.
In the said step a), section 4 and imaging system 5 relatively continuous motions in the horizontal, that is: or imaging system 5 static, section 4 motions; Or imaging system 5 motion, it is 4 static to cut into slices.
In the said step a), section 4 and imaging system 5 continuous motion relatively in the horizontal, or uniform motion, or non-uniform movement; As long as the speed of related movement in guaranteeing between imaging system 5 exposure periods can not cause image blurring.
The time for exposure of imaging system 5 is to confirm according to the section 4 and the speed of related movement of imaging system 5, as long as in guaranteeing between imaging system 5 exposure periods, section 4 can not cause image blurring getting final product with the speed of imaging system 5 relative motions.
Time for exposure the best of imaging system 5 is elected 20 μ s as.
The above; It only is the preferred embodiment of the utility model; Be not to be the restriction of the utility model being made other form, any professional and technical personnel of being familiar with possibly utilize the technology contents of above-mentioned announcement to change or be modified as the equivalent embodiment of equivalent variations.But every the utility model technical scheme content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment did, still belongs to the protection range of the utility model technical scheme according to the technical spirit of the utility model.
Claims (13)
1. the scanning means of a microsection comprises computer, it is characterized in that: also comprise illuminator, the imaging system of coaxial setting, between said illuminator and the imaging system, also be provided with an automatic three-dimensional mobile platform; One controller is controlled said automatic three-dimensional mobile platform motion, can place section to be measured on the said automatic three-dimensional mobile platform; Said computer and said controller, imaging system electrically connect respectively each other, control said imaging system and control said automatic three-dimensional mobile platform synchronous working through said controller; And make the section to be measured and the imaging system of placing on the said automatic three-dimensional mobile platform be in the continuous relative motion.
2. the scanning means of microsection according to claim 1 is characterized in that: on the said automatic three-dimensional mobile platform, between said automatic three-dimensional mobile platform and the imaging system, be provided with the section bracing frame; Said section bracing frame is used to place section to be measured.
3. the scanning means of microsection according to claim 2; It is characterized in that: said controller is controlled automatic three-dimensional mobile platform and is driven the section bracing frame and go up section to be measured, at the same time or separately directions X promptly laterally, the Y direction promptly vertically and the Z direction be axial motion.
4. according to the scanning means of the arbitrary described microsection of claim 1 to 3, it is characterized in that: described imaging system comprises imaging lens and the imageing sensor that coaxial order is provided with.
5. the scanning means of microsection according to claim 4 is characterized in that: described imaging lens comprises object lens and the imaging len that coaxial order is provided with.
6. the scanning means of microsection according to claim 5, it is characterized in that: said imageing sensor is an array image sensor; Described array image sensor, its minimum exposure time are the μ s order of magnitude.
7. the scanning means of microsection according to claim 6, it is characterized in that: described illuminator comprises illuminating lens and light source.
8. the scanning means of a microsection comprises computer, it is characterized in that: also comprise illuminator, section bracing frame, imaging system that coaxial successively order is provided with, said section bracing frame is fixed, can place section to be measured on it; Said imaging system is connected with an automatic three-dimensional mobile platform; Said automatic three-dimensional mobile platform is controlled by a controller, can drive said imaging system motion; Said computer electrically connects with said controller, imaging system respectively each other, controls said automatic three-dimensional mobile platform synchronous working by the said imaging system of said computer control and through said controller; And make the section to be measured of placing on said imaging system and the said section bracing frame be in the continuous relative motion.
9. the scanning means of microsection according to claim 8, it is characterized in that: said controller is controlled automatic three-dimensional mobile platform, drives said imaging system, at the same time or separately directions X promptly laterally, the Y direction promptly vertically and the Z direction be axial motion.
10. it is characterized in that according to Claim 8 or the scanning means of 9 described microsections: described imaging system comprises imaging lens and the imageing sensor that coaxial order is provided with.
11. the scanning means of microsection according to claim 10 is characterized in that: described imaging lens comprises object lens and the imaging len that coaxial order is provided with.
12. the scanning means of microsection according to claim 11 is characterized in that: said imageing sensor is an array image sensor; Described array image sensor, its minimum exposure time are the μ s order of magnitude.
13. the scanning means of microsection according to claim 12 is characterized in that: described illuminator comprises light source and the illuminating lens that coaxial order is provided with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203825525U CN202231776U (en) | 2011-10-01 | 2011-10-01 | Microsection scanning device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203825525U CN202231776U (en) | 2011-10-01 | 2011-10-01 | Microsection scanning device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202231776U true CN202231776U (en) | 2012-05-23 |
Family
ID=46082348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203825525U Expired - Lifetime CN202231776U (en) | 2011-10-01 | 2011-10-01 | Microsection scanning device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202231776U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103175780A (en) * | 2013-03-20 | 2013-06-26 | 上海交通大学 | Automated imaging system of large-size serial section of biological tissue |
| CN105004723A (en) * | 2015-06-25 | 2015-10-28 | 宁波江丰生物信息技术有限公司 | Pathological section scanning 3D imaging and fusion device and method |
| CN106918484A (en) * | 2017-03-28 | 2017-07-04 | 武汉瑞福宁科技有限公司 | A kind of construction method of the threedimensional model based on histotomy |
| CN107426464A (en) * | 2017-09-01 | 2017-12-01 | 上海极清慧视科技有限公司 | A kind of pathology print scanning shoot method using ERS sensors |
| CN110006684A (en) * | 2019-03-13 | 2019-07-12 | 广州金域医学检验中心有限公司 | The generation system and method for digital pathological section |
| CN110836891A (en) * | 2018-08-17 | 2020-02-25 | 湖南爱威医疗科技有限公司 | Method, device and equipment for realizing rapid microscopic examination and computer readable storage medium |
| CN113376138A (en) * | 2020-08-11 | 2021-09-10 | 中国科学院物理研究所 | Microscope three-dimensional moving focusing system and application |
-
2011
- 2011-10-01 CN CN2011203825525U patent/CN202231776U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103175780A (en) * | 2013-03-20 | 2013-06-26 | 上海交通大学 | Automated imaging system of large-size serial section of biological tissue |
| CN105004723A (en) * | 2015-06-25 | 2015-10-28 | 宁波江丰生物信息技术有限公司 | Pathological section scanning 3D imaging and fusion device and method |
| CN106918484A (en) * | 2017-03-28 | 2017-07-04 | 武汉瑞福宁科技有限公司 | A kind of construction method of the threedimensional model based on histotomy |
| CN107426464A (en) * | 2017-09-01 | 2017-12-01 | 上海极清慧视科技有限公司 | A kind of pathology print scanning shoot method using ERS sensors |
| CN110836891A (en) * | 2018-08-17 | 2020-02-25 | 湖南爱威医疗科技有限公司 | Method, device and equipment for realizing rapid microscopic examination and computer readable storage medium |
| CN110006684A (en) * | 2019-03-13 | 2019-07-12 | 广州金域医学检验中心有限公司 | The generation system and method for digital pathological section |
| CN113376138A (en) * | 2020-08-11 | 2021-09-10 | 中国科学院物理研究所 | Microscope three-dimensional moving focusing system and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102427502B (en) | Scanning method and device for microscopic section | |
| CN202231776U (en) | Microsection scanning device | |
| CN102854615B (en) | Full-automatic scanning system and method for microscopic section | |
| CN106583941B (en) | 3D body laser inner carving method and system based on mobile end data acquisition | |
| CN105988209B (en) | One kind being based on microscopical automatic detection system | |
| CN105683805B (en) | The image acquisition method of image capturing device and image capturing device | |
| CN102109673A (en) | Stage control device, stage control method, stage control program, and microscope | |
| CN102814874B (en) | Processing unit (plant) | |
| CN104833659B (en) | Bio-sample tomography micro-imaging system | |
| WO2000057231A1 (en) | Scanning confocal microscope | |
| CN108387517A (en) | It is sliced scan method and system | |
| CN106645045B (en) | TDI-CCD-based bidirectional scanning imaging method in fluorescence optical microscopy imaging | |
| CN101883198B (en) | Scanner capable of detecting in real time and physically adjusting frame and scanning method thereof | |
| CN111033352B (en) | Image acquisition device and image acquisition method | |
| CN108551538B (en) | Platform movable type wide-width industrial scanning equipment and scanning method | |
| CN109061865A (en) | It is a kind of can auto-focusing more multiplying power microscopic structures | |
| CN111203642A (en) | Curved surface laser marking machine | |
| CN104019761A (en) | Three-dimensional configuration obtaining device and method of corn plant | |
| CN210720869U (en) | Cell micro-microscopic image acquisition device | |
| CN205787345U (en) | A kind of based on microscopical automatic detection system | |
| CN208477200U (en) | It is a kind of can auto-focusing the micro- scanning system of zoom | |
| CN217010940U (en) | High-resolution scanning splicing imaging device | |
| CN106506972B (en) | A kind of printed circuit board checking device | |
| CN215868286U (en) | Machine vision teaching experiment platform of linear array scanning type | |
| US9917996B2 (en) | Image pickup device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120523 |
|
| CX01 | Expiry of patent term |