CN201746538U

CN201746538U - Compound-bacteria mixed cultivating device

Info

Publication number: CN201746538U
Application number: CN 201020279489
Authority: CN
Inventors: 丛方地; 刘志辉; 黄健; 苏永鹏; 王洪杰
Original assignee: Great Biology Pharmaceutical Tianjin Co Ltd
Current assignee: Great Biology Pharmaceutical Tianjin Co Ltd
Priority date: 2010-08-02
Filing date: 2010-08-02
Publication date: 2011-02-16
Anticipated expiration: 2020-08-02

Abstract

The utility model relates to a compound-bacteria mixed cultivating device, which is composed of a fermentor body, a sealing cover and a stirring rod, wherein the sealing cover is coaxially installed on the fermentor body, and the stirring rod is installed in the middle shaft position of the sealing cover; the lower end of the stirring rod is provided with a stirring blade, and the sealing cover is provided with a feed inlet; the lower bottom part of the fermentor body is provided with a discharge outlet, and the cylindrical-surface upper part of the fermentor body is respectively provided with an air inlet pipe and an air outlet pipe; and the fermentor body is in a double-layer hollow cylinder structure with an interlayer. The cylindrical surface of the fermentor body under the air inlet pipe is provided with an interlayer air inlet pipe and an interlayer air outlet pipe, and the middle lower part in the interlayer of the fermentor body is wound with a spiral water pipe. The spiral water pipe is respectively communicated with a spiral water inlet pipe and a spiral water outlet pipe which are installed on the cylindrical surface of the fermentor body. The device realizes the development and utilization of compound bacteria on aquiculture and has the advantages of equipment saving, convenience, fastness and good effect. The compound bacteria are cultivated for 24-26 hours at the constant temperature of 30-37 DEG C, and the detected concentration of each kind of compound bacteria reaches 108-1010 pieces per mL. The device improves the healthy development of aquaculture industry and compensates for the blank of domestic anaerobic bacteria and aerobes organic cooperation cultivating devices.

Description

A kind of composite bacteria mixed culture device

Technical field

The utility model belongs to the fermentation field, relates to a kind of composite bacteria mixed culture device.

Background technology

Along with rapid economy development, people's standard of living improves greatly, and people also strengthen gradually to food safety and environmental protection consciousness, and economic Sustainable development has been pendulum the only way in face of us.Current in culture fishery, along with the continuous expansion of propagating scale artificially, breeding environment worsens day by day, and breaking out of disease is day by day frequent, has greatly suppressed the growth of aquaculture output and the development of aquatic products trade.Microbiotic has been used for many years as fodder additives, at the breeding that suppresses pathogenic micro-organism, minimizing metabolism toxin the aspects such as toxic action of animal have all been played active effect, but because antibiotic life-time service and abuse, its all drawback reveals gradually, as cause the animal gastrointestinal tract normal microflora to lack of proper care, animal body immunizing power is produced detrimentally affect, produces side effects such as resistance and drug residue, brought potential hazard for thus aquaculture and human consumer's health, had a strong impact on the quality and the safety of fishery products, and the safety of water surrounding.Of common occurrence because of the food safety case that drug residue causes, cause China's fishery products competitive power in the international market to weaken greatly, the nuisanceless green fishery products of therefore developing safety, high-quality, nutrition have become the common recognition of current society.

The immunity system of probiotics by stimulating body, regulating in the body in microecological balance, antagonism pathogenic microorganism, the degrading cultivation process organic waste and wait to reach and improve immunizing power, disease-resistant, promotes growth and effect such as purify water, is the green used for aquiculture product that a class has development potentiality greatly.Aerobic at present and anaerobism micro-ecological composite bacterium prepares, and is mostly to cultivate respectively the back remix, like this, expends more equipment and time, and is unfavorable for setting up between the various bacterium a kind of microecosystem of symbiotic co-existence.The mixed culture of composite bacteria generally has certain requirement for bacterial classification and culture condition, is difficult to the feeding T/A that other cooperates culture condition control oxygen, is not suitable for large-scale farming and uses.

Summary of the invention

The purpose of this utility model is to overcome the deficiencies in the prior art part, provide a kind of simple in structure, cost is low, easy operation, produce the high composite bacteria mixed culture device of bacterium efficient.

The utility model solves its technical problem and takes following technical scheme to realize:

A kind of composite bacteria mixed culture device, by the fermentation body, sealing cover and agitator arm constitute, sealing cover co-axial seal lid dress fermentation body, agitator arm is installed in sealing cover axis position, its lower end is equipped with agitating vane, opening for feed also is installed on sealing cover, lower bottom part at the fermentation body is equipped with discharge port, cylinder top at the fermentation body is separately installed with inlet pipe and escape pipe, described fermentation body is the double-deck hollow circuit cylinder body structure that has interlayer, one interlayer water inlet pipe and interlayer rising pipe are installed on the cylinder of the fermentation body below the inlet pipe, the position, middle and lower part is wound with pipe with spiral pipe in the fermentation container interlayer body, and this pipe with spiral pipe is communicated with spiral water inlet pipe and the spiral rising pipe that is installed on the fermentation container scapus face respectively.

And, describedly on the cylinder of fermentation body, its front end also is installed gos deep into the intravital thermometer of fermentation container.

And described fermentation body bottom is shaped on leg.

Advantage of the present utility model and positively effect are:

1, in this device, micro-ecological composite bacterium preparation comprises aerobic subtilis and amphimicrobian milk-acid bacteria and yeast, three kinds of probiotic bacteriums can be set up good coexistence system in this device, can carry out high-efficient culture by organic control of installing agitator own, ventilating pit, limbers, input and output material hole, form the equilibrated microecosystem, helped the symbiotic co-existence of mushroom.

2, this device has been realized the development and use of composite bacteria in aquaculture, saving equipment, convenient and swift, effect is better, through calculating, at 30-37 ℃ of constant temperature culture 24-26 hour, each bacterial classification detectable level reached 10 ⁸-10 ¹⁰Individual/mL, promote culture fishery to develop in a healthy way, remedied the blank of domestic anerobe, the organic cooperation culture apparatus of aerophil.

Description of drawings

Fig. 1 is the utility model structure cross-sectional schematic.

Embodiment

The utility model is described in further detail below in conjunction with accompanying drawing and by specific embodiment, and following examples are descriptive, are not determinate, can not limit protection domain of the present utility model with this.

A kind of composite bacteria mixed culture device, constitute by fermentation body 7, sealing cover 1 and agitator arm 3, the fermentation body is the double-deck hollow circuit cylinder body structure that has interlayer, sealing cover co-axial seal lid dress fermentation body, agitator arm is installed in sealing cover axis position, and its lower end is equipped with agitating vane 13, and agitator arm can adopt electric motor driving to rotate, opening for feed 2 also is installed on sealing cover, at the lower bottom part of fermentation body discharge port 11 is installed and is shaped on leg 10 in fermentation body bottom.

Cylinder top at the fermentation body is separately installed with inlet pipe 4 and escape pipe 15, and inlet pipe connects cleaning, appropriate suitable air transmission mechanism, and device starts, and carries air with certain speed, carries out the cultivation of aerobic bacteria; One interlayer water inlet pipe 5 and interlayer rising pipe 12 also are installed on the cylinder of the fermentation body below the inlet pipe, are used in the interlayer of fermentation body, intaking and discharging water; The position, middle and lower part is wound with pipe with spiral pipe 8 in the fermentation container interlayer body, this pipe with spiral pipe is communicated with spiral water inlet pipe 9 and the spiral rising pipe 6 that is installed on the fermentation container scapus face respectively, pipe with spiral pipe connects adjustable thermostatic recirculated water heating installation by the spiral water inlet pipe, water in the pipe with spiral pipe is constantly circulated, make in the fermentation body by the interbedded water in the interlayer to keep constant temperature.

On the cylinder of fermentation body, its front end is installed also and gos deep into the intravital thermometer 14 of fermentation container.

The use embodiment of this device is as follows:

The volume of fermentation body can design according to the size of turnout, and generally between 1L-100L, reaction system accounts for volumetrical 1/3-1/2; The cultivation and fermentation body will carry out pre-constant temperature by pipe with spiral pipe before adding bacterial classification, and then adds spawn culture; Air flow is controlled at 100-200L/min; Stirring velocity is controlled at 5-20r/min; Advanced aerobic cultivation 10-11h stops ventilation then and continues stir culture 14-16h; Culture temperature remains on 30-37 ℃, and the content of each bacterium is all at 108-1010/mL in the last gained composite bacteria product.The mass ratio of the sodium-chlor of culture system formula ratio, nitrogenous source, agar, carbon source is a sodium-chlor: nitrogenous source: agar: carbon source=3-6: 1-4: 300: 2-4.

Use embodiment 1:

Volume after sterilization is in the culture apparatus of 3L, add pure water 850mL, agar 30g, glucose (carbon is former) 0.3g, sodium-chlor 0.5g, yeast extract powder (nitrogen is former) 0.3g, cover sealing cover, start agitator arm then, begin to stir, start pipe with spiral pipe subsequently and carry out the constant temperature circulation with 20r/min, at 33 ℃ of constant temperature 30min, be subtilis 1 * 10 with concentration ³Individual/mL, milk-acid bacteria 1 * 10 ⁴Individual/mL, yeast 1 * 10 ³Each 50mL of individual/mL adds and the sealing charging opening from charging opening; Ventilation, 150L/min behind the 10h, stops ventilation, and rotating speed is adjusted into 6r/min, cultivates 15h, stops to stir and circulating water system, opens bacterial classification and adds inlet, emits product from discharge port.

Use embodiment 2:

Volume after sterilization is in the culture apparatus of 3L, add pure water 850mL, agar 30g, glucose (carbon is former) 0.3g, sodium-chlor 0.5g, yeast extract powder (nitrogen is former) 0.3g, cover sealing cover, start stirring system then, begin to stir, start pipe with spiral pipe subsequently and carry out the constant temperature circulation with 13r/min, at 37 ℃ of constant temperature 30min, be subtilis 1 * 10 with concentration ³Individual/mL, milk-acid bacteria 1 * 10 ⁴Individual/mL, yeast 1 * 10 ³Each 50mL of individual/mL adds and the sealing charging opening from charging opening; Ventilation, 140L/min behind the 11h, stops ventilation, and rotating speed is adjusted into 5r/min, cultivates 13h, stops to stir and circulating water system, opens bacterial classification and adds inlet, emits product from discharge port.

Claims

1. composite bacteria mixed culture device, by the fermentation body, sealing cover and agitator arm constitute, sealing cover co-axial seal lid dress fermentation body, agitator arm is installed in sealing cover axis position, its lower end is equipped with agitating vane, opening for feed also is installed on sealing cover, lower bottom part at the fermentation body is equipped with discharge port, cylinder top at the fermentation body is separately installed with inlet pipe and escape pipe, it is characterized in that: described fermentation body is the double-deck hollow circuit cylinder body structure that has interlayer, one interlayer water inlet pipe and interlayer rising pipe are installed on the cylinder of the fermentation body below the inlet pipe, the position, middle and lower part is wound with pipe with spiral pipe in the fermentation container interlayer body, and this pipe with spiral pipe is communicated with spiral water inlet pipe and the spiral rising pipe that is installed on the fermentation container scapus face respectively.

2. composite bacteria mixed culture device according to claim 1 is characterized in that: describedly its front end also is installed gos deep into the intravital thermometer of fermentation container on the cylinder of fermentation body.

3. composite bacteria mixed culture device according to claim 1 is characterized in that: described fermentation body bottom is shaped on leg.