CN108018209A - One kind culture cell and strain device - Google Patents
One kind culture cell and strain device Download PDFInfo
- Publication number
- CN108018209A CN108018209A CN201810130817.9A CN201810130817A CN108018209A CN 108018209 A CN108018209 A CN 108018209A CN 201810130817 A CN201810130817 A CN 201810130817A CN 108018209 A CN108018209 A CN 108018209A
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- Prior art keywords
- accommodating chamber
- led
- pipe fitting
- pipe
- chamber
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses one kind culture cell and strain device, including culture apparatus and attemperator, culture apparatus includes matrix A, described matrix A lower faces are installed with support foot, accommodating chamber A is provided with described matrix A, the screwed pipe in described matrix A is provided with the accommodating chamber A appearance walls, the screwed pipe right-side outlet leads to the tube channel for being provided with and extending outside to the right, the screwed pipe right end leads to the accommodating chamber A, the tube channel right side, which is led to, is provided with driven valve, the driven valve right side, which is led to, is provided with the first connecting leg, apparatus of the present invention are simple in structure, it is easy to use, this equipment is heated and kept the temperature using immersion method, steady change amplitude is small, isoperibol can be provided to greatest extent, be conducive to cell and Spawn incubation.
Description
Technical field
The present invention relates to biological field, and in particular to one kind culture cell and strain device.
Background technology
The culture of cell and strain often requires and carries out at a certain temperature that traditional incubator needs directly to utilize
Electrothermal tube fever is to maintain the temperature in incubator, and the consumption energy is big and temperature is difficult to control, and temperature is easily excessive or too low, temperature
Degree change is violent, is unfavorable for the culture of cell and strain.
The content of the invention
It is an object of the invention to provide one kind culture cell and strain device, and the above-mentioned of the prior art can be overcome to lack
Fall into.
According to the present invention, a kind of the culture cell and strain device of apparatus of the present invention, including culture apparatus and attemperator,
Culture apparatus includes matrix A, and described matrix A lower faces are installed with support foot, and accommodating chamber A, the appearance are provided with described matrix A
Receive and be provided with the screwed pipe in described matrix A in chamber A appearance walls, the screwed pipe right-side outlet, which leads to be provided with, to extend to the right
Exterior tube channel, the screwed pipe right end lead to the accommodating chamber A, and the tube channel right side, which is led to, is provided with driven valve,
The driven valve right side, which is led to, is provided with the first connecting leg, and the screwed pipe left side outlet, which leads to be provided with, extends to the left outside
Pipe fitting A, the pipe fitting A left sides, which are led to, is provided with gate valve A, and the gate valve A left sides, which are led to, is provided with pipe fitting B, described matrix A
Upper surface is installed with the storage case in the accommodating chamber A, and the storage case lower face is installed with and the accommodating chamber A lower ends
The matched backing plate of wall, is provided with accommodating chamber B in the storage case, the accommodating chamber B left end walls are installed with inductor, the appearance
Receive the metal tube for leading in chamber B lower end walls and being provided with and penetrating through the accommodating chamber A lower end walls and outside stretching out, the metal tube upper end
Face is installed with gate valve H, and the metal tube left and right sides is provided with the spring coil chamber in described matrix A, the spring coil chamber outer end wall
Inside be provided with and slide chamber, it is described slide that intracavitary can slide be provided with and slide bar, the bar that slides is installed with and institute towards inner face
The push plate that end wall above and below spring coil chamber slides mating connection is stated, spring coil is provided between the push plate and the spring coil chamber outer end wall,
The push plate inward bottom wall is installed with fixing end, and the fixing end is engaged with the fixing groove being arranged in the metal tube, institute
State metal tube lower face and lead to and be provided with gate valve D, the gate valve D lower faces, which are led to, is provided with soft tube, and first connecting leg connects
Pipe fitting is connected to, the pipe fitting D right sides, which are led to, is provided with electronic control valve, and the electronic control valve right side, which is led to, is provided with pipe
Part E, the pipe fitting E right sides, which are led to, is provided with suction pump, and the suction pump right side, which is led to, is provided with downward extended pipe fitting
Attemperator is provided with below F, the pipe fitting F, attemperator includes the matrix B being fixedly arranged on ground, set in described matrix B
Have and the suction head led to the pipe fitting F is provided with accommodating chamber C, the accommodating chamber C, it is fixed in the accommodating chamber C inner bottom walls
Heat-generating pipe is provided with, the heat-generating pipe is electrically connected to a power source.
Further technical solution, the screwed pipe are spiral, described in the screwed pipe winding in accommodating chamber A appearance walls, so that
Improve the heat transfer efficiency of equipment.
The beneficial effects of the invention are as follows：
Apparatus of the present invention are simple in structure, easy to use, this equipment is heated and kept the temperature using immersion method, steady change amplitude
It is small, isoperibol can be to greatest extent provided, be conducive to cell and Spawn incubation.
Brief description of the drawings
, below will be to embodiment or existing in order to illustrate more clearly of inventive embodiments or technical solution of the prior art
Attached drawing is briefly described needed in technology description, it should be apparent that, drawings in the following description are only to invent
Some embodiments, for those of ordinary skill in the art, without creative efforts, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is culture apparatus structure diagram in the present invention；
Fig. 2 is attemperator structure diagram in the present invention.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
This specification（Including any accessory claim, summary and attached drawing）Disclosed in any feature, except non-specifically chatting
State, can be replaced by other alternative features that are equivalent or have similar purpose.I.e., unless specifically stated, each feature
It is an example in a series of equivalent or similar characteristics.
As shown in Figs. 1-2, a kind of culture cell and strain device of the invention, including culture apparatus and attemperator, training
Supporting device includes matrix A101, and described matrix A101 lower faces are installed with support foot 100, receiving is provided with described matrix A101
Chamber A106, the screwed pipe 105 in described matrix A101 is provided with the accommodating chamber A106 appearance walls, and the screwed pipe 105 is right
Side outlet leads to the tube channel 122 for being provided with and extending outside to the right, and 105 right end of screwed pipe leads to the accommodating chamber A106
Even, 122 right side of tube channel, which is led to, is provided with driven valve 121, and 121 right side of driven valve, which is led to, is provided with the first company
Pipe 120,105 left side outlet of screwed pipe lead to the pipe fitting A102 for being provided with and extending outside to the left, the pipe fitting A102 left ends
Face, which is led to, is provided with gate valve A103, and the gate valve A103 left sides, which are led to, is provided with pipe fitting B104, described matrix A101 upper surfaces
The storage case 107 being installed with the accommodating chamber A106,107 lower face of storage case is installed with and the accommodating chamber
The matched backing plate 112 of A106 lower end walls, is provided with accommodating chamber B110, the accommodating chamber B110 left ends in the storage case 107
Wall is installed with inductor 111, lead in the accommodating chamber B110 lower end walls be provided with the perforation accommodating chamber A106 lower end walls and
Metal tube 128 outside stretching out, 128 upper surface of metal tube are installed with gate valve H113, and 128 left and right sides of metal tube is set
There is the spring coil chamber 126 in described matrix A101, be provided with 126 outer end wall of spring coil chamber and slide chamber 124, it is described to slide
Bar 125 is slided in being provided with for being slided in chamber 124, and the bar 125 that slides is installed with the spring coil chamber about 126 towards inner face
End wall slides the push plate 123 of mating connection, and spring coil is provided between the push plate 123 and 126 outer end wall of spring coil chamber, described
123 inward bottom wall of push plate is installed with fixing end 127, the fixing end 127 and the fixing groove phase being arranged in the metal tube 128
Coordinate, 128 lower face of metal tube, which is led to, is provided with gate valve D131, and the gate valve D131 lower faces, which are led to, is provided with soft tube
129, first connecting leg 120 is connected to pipe fitting 115, and the pipe fitting D115 right sides, which are led to, is provided with electronic control valve 116, institute
State 116 right side of electronic control valve and lead to and be provided with pipe fitting E117, the pipe fitting E117 right sides, which are led to, is provided with suction pump
118,118 right side of suction pump, which is led to, is provided with downward extended pipe fitting F119, and guarantor is provided with below the pipe fitting F119
Warm device, attemperator include being fixedly arranged on matrix B136 on ground, and accommodating chamber C137 is provided with described matrix B136, described
The suction head 144 led to the pipe fitting F119 is provided with accommodating chamber C137, fixes and sets in the accommodating chamber C137 inner bottom walls
Heat-generating pipe 135 is equipped with, the heat-generating pipe 135 is electrically connected to a power source.
Valuably, wherein, the screwed pipe 105 is spiral, described in 105 winding of screwed pipe in accommodating chamber A106 appearance walls,
So as to improve the heat transfer efficiency of equipment.
In original state, the gate valve A, electronic control valve, driven valve, gate valve D and gate valve H are in close present device
Closed state, the suction pump are in stop working state, so as to be conducive to the maintenance and installation of equipment.
When equipment is run, the gate valve A, electronic control valve 116 and driven valve are opened, and the heat-generating pipe 135 is powered, right
Water in the accommodating chamber C137 is heated, and water is sucked through pipe fitting F119, suction pump 118, pipe by the suction head 144
Part E117, pipe fitting D115, first connecting leg 120, the tube channel 122 enter the accommodating chamber A106 and are full of the screwed pipe
105, heat is delivered in the accommodating chamber B by the accommodating chamber A, and the accommodating chamber B is heated and kept the temperature, the sensing
Device perceives the temperature in the accommodating chamber B so as to conveniently be controlled, so as to carry out the training of cell and strain for the accommodating chamber B
Support the environment that constant temperature is provided；
When needing to discharge the water in the accommodating chamber A106, the gate valve A103 is opened, the accommodating chamber A106 and the screwed pipe
Water in 105 is discharged from pipe fitting A；
Cell is carried out when directly filling liquid in accommodating chamber B110 and during Spawn incubation, and culture terminates to open gate valve H113 and described
Gate valve D131 discharges liquid.
The beneficial effects of the invention are as follows：
Apparatus of the present invention are simple in structure, easy to use, this equipment is heated and kept the temperature using immersion method, steady change amplitude
It is small, isoperibol can be to greatest extent provided, be conducive to cell and Spawn incubation.
The above, the embodiment only invented, but invent protection domain be not limited thereto, it is any without
The change or replacement that creative work is expected are crossed, should all be covered within the protection domain of invention.Therefore, the protection domain of invention
It should be determined by the scope of protection defined in the claims.
Claims (2)
1. one kind culture cell and strain device, including culture apparatus and attemperator, culture apparatus include matrix A, the base
Body A lower faces are installed with support foot, and accommodating chamber A is provided with described matrix A, is provided with and is located in the accommodating chamber A appearance walls
Screwed pipe in described matrix A, the screwed pipe right-side outlet lead to the tube channel for being provided with and extending outside to the right, and the screwed pipe is right
End is led to the accommodating chamber A, and the tube channel right side, which is led to, is provided with driven valve, and setting is led in the driven valve right side
There is the first connecting leg, the screwed pipe left side outlet leads to the pipe fitting A for being provided with and extending outside to the left, and the pipe fitting A left sides lead to
It is connected with and is equipped with gate valve A, the gate valve A left sides, which are led to, is provided with pipe fitting B, and described matrix A upper surfaces are installed with positioned at the appearance
The storage case received in chamber A, the storage case lower face are installed with and the matched backing plate of accommodating chamber A lower end walls, the appearance
Receive and be provided with accommodating chamber B in case, the accommodating chamber B left end walls are installed with inductor, lead to setting in the accommodating chamber B lower end walls
There is the metal tube penetrated through outside the accommodating chamber A lower end walls and stretching, the metal tube upper surface is installed with gate valve H, the gold
Belong to pipe left and right sides and be provided with the spring coil chamber in described matrix A, be provided with the spring coil chamber outer end wall and slide chamber, the cunning
Bar is slided in being provided with for being slided in an actor's rendering of an operatic tune, it is described slide bar and be installed with to slide with end wall above and below the spring coil chamber towards inner face match somebody with somebody
The push plate of connection is closed, spring coil is provided between the push plate and the spring coil chamber outer end wall, the push plate inward bottom wall is installed with
Fixing end, the fixing end are engaged with the fixing groove being arranged in the metal tube, and setting is led in the metal tube lower face
There is gate valve D, the gate valve D lower faces, which are led to, is provided with soft tube, and first connecting leg is connected to pipe fitting, the pipe fitting D right ends
Face, which is led to, is provided with electronic control valve, and the electronic control valve right side, which is led to, is provided with pipe fitting E, and the pipe fitting E right sides lead to
It is connected with and is equipped with suction pump, the suction pump right side, which is led to, is provided with downward extended pipe fitting F, is provided with below the pipe fitting F
Attemperator, attemperator include the matrix B being fixedly arranged on ground, accommodating chamber C, the accommodating chamber C are provided with described matrix B
The suction head led to the pipe fitting F is inside provided with, heat-generating pipe, the fever are fixedly installed in the accommodating chamber C inner bottom walls
Pipe is electrically connected to a power source.
2. a kind of culture cell as claimed in claim 1 and strain device, it is characterised in that：The screwed pipe is spiral, described
Described in screwed pipe winding in accommodating chamber A appearance walls.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130817.9A CN108018209B (en) | 2018-02-08 | 2018-02-08 | A kind of cell and bacterial strain culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130817.9A CN108018209B (en) | 2018-02-08 | 2018-02-08 | A kind of cell and bacterial strain culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108018209A true CN108018209A (en) | 2018-05-11 |
| CN108018209B CN108018209B (en) | 2018-09-28 |
Family
ID=62075346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810130817.9A Expired - Fee Related CN108018209B (en) | 2018-02-08 | 2018-02-08 | A kind of cell and bacterial strain culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108018209B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117801954A (en) * | 2024-01-08 | 2024-04-02 | 山东诺心生物科技有限公司 | Bionic culture device for embryonic stem cells |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3980131A (en) * | 1975-05-08 | 1976-09-14 | New Brunswick Scientific Co., Inc. | Sterilizer for culture media and laboratory ware |
| CN201729836U (en) * | 2010-07-14 | 2011-02-02 | 中国人民解放军第四军医大学 | Cell culture dish convenient for perfusion and heat insulation |
| CN201746538U (en) * | 2010-08-02 | 2011-02-16 | 格特生物制药（天津）有限公司 | Compound-bacteria mixed cultivating device |
| CN203065486U (en) * | 2013-01-22 | 2013-07-17 | 桂林电子科技大学 | Device for rapid enrichment-proliferation and purification-cultivation for anaerobic ammonium oxidation bacteria |
| CN204482428U (en) * | 2015-02-17 | 2015-07-22 | 湖南和平生物科技有限公司 | A kind of strain cultivation tank |
| CN206279212U (en) * | 2016-12-01 | 2017-06-27 | 广州美岸生物科技有限公司 | A kind of cell culture apparatus |
| CN206308374U (en) * | 2016-12-30 | 2017-07-07 | 宁波键一生物科技有限公司 | A kind of fully-automatic intelligent cell culture apparatus |
| CN206736259U (en) * | 2017-05-03 | 2017-12-12 | 天津鸿泽天创生物环保科技发展有限公司 | A kind of automatic manufacturing device for being adapted to a large amount of probiotics fermentions |
| CN206927896U (en) * | 2017-06-07 | 2018-01-26 | 塔里木大学 | A kind of special Prevention of Infectious Diseases thermostatic type strain culturing device of animal doctor |
-
2018
- 2018-02-08 CN CN201810130817.9A patent/CN108018209B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3980131A (en) * | 1975-05-08 | 1976-09-14 | New Brunswick Scientific Co., Inc. | Sterilizer for culture media and laboratory ware |
| CN201729836U (en) * | 2010-07-14 | 2011-02-02 | 中国人民解放军第四军医大学 | Cell culture dish convenient for perfusion and heat insulation |
| CN201746538U (en) * | 2010-08-02 | 2011-02-16 | 格特生物制药（天津）有限公司 | Compound-bacteria mixed cultivating device |
| CN203065486U (en) * | 2013-01-22 | 2013-07-17 | 桂林电子科技大学 | Device for rapid enrichment-proliferation and purification-cultivation for anaerobic ammonium oxidation bacteria |
| CN204482428U (en) * | 2015-02-17 | 2015-07-22 | 湖南和平生物科技有限公司 | A kind of strain cultivation tank |
| CN206279212U (en) * | 2016-12-01 | 2017-06-27 | 广州美岸生物科技有限公司 | A kind of cell culture apparatus |
| CN206308374U (en) * | 2016-12-30 | 2017-07-07 | 宁波键一生物科技有限公司 | A kind of fully-automatic intelligent cell culture apparatus |
| CN206736259U (en) * | 2017-05-03 | 2017-12-12 | 天津鸿泽天创生物环保科技发展有限公司 | A kind of automatic manufacturing device for being adapted to a large amount of probiotics fermentions |
| CN206927896U (en) * | 2017-06-07 | 2018-01-26 | 塔里木大学 | A kind of special Prevention of Infectious Diseases thermostatic type strain culturing device of animal doctor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117801954A (en) * | 2024-01-08 | 2024-04-02 | 山东诺心生物科技有限公司 | Bionic culture device for embryonic stem cells |
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| Publication number | Publication date |
|---|---|
| CN108018209B (en) | 2018-09-28 |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180816 Address after: 200120 C, 888, west two road, Nanhui new town, Pudong New Area, Shanghai Applicant after: IMPROVING LIFE BIOTECHNOLOGY (SHANGHAI) CO., LTD. Address before: 312399 Shaoxing, Shangyu, Zhejiang Shangyu District, the new village village street, Buddha Hall piece No. 3 Applicant before: Shangyu Shaoxing Zhongtai medical science and Technology Co., Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180928 Termination date: 20200208 |