CN102174373A - Microcystis aeruginosa research device - Google Patents

Microcystis aeruginosa research device Download PDF

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Publication number
CN102174373A
CN102174373A CN2011100500102A CN201110050010A CN102174373A CN 102174373 A CN102174373 A CN 102174373A CN 2011100500102 A CN2011100500102 A CN 2011100500102A CN 201110050010 A CN201110050010 A CN 201110050010A CN 102174373 A CN102174373 A CN 102174373A
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tank
microcystic aeruginosa
arm beam
research device
water
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CN102174373B (en
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楼春华
李其军
杨淑慧
张春义
顾华
陈英硕
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BEIJING INST OF WATER CONSERVANCY SCIENCE
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BEIJING INST OF WATER CONSERVANCY SCIENCE
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/02Means for providing, directing, scattering or concentrating light located outside the reactor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

The invention discloses a microcystis aeruginosa research device which comprises a water tank, a motor and a lighting system, wherein the water tank is in the shape of an annular column with an upward opening; the motor is arranged in the center of circle of the water tank; an output shaft of the motor is provided with vertical supporting beams; the top ends of the supporting beams are provided with horizontal suspension arm beams; water pushing sheets are arranged below the suspension arm beams and extend into the water tank; and the lighting system is arranged at the periphery of the water tank. The device has the characteristic that a water body has basically consistent flow velocity during an experiment.

Description

Microcystic aeruginosa class research device
Technical field
The present invention relates to a kind of biological study device, particularly relate to a kind of microcystic aeruginosa class research device.
Background technology
From achievement in research in recent years, there is certain relation in the flow velocity size with wawter bloom generation speed, increase flow velocity and can effectively suppress wawter bloom and obtained sufficient checking, but but there is very big-difference in the size of critical flow velocity value.From research process, the present following problem of experimental study ubiquity:
(1) experimental scale is less than normal.
From existing experimental study, testing apparatus adopts the baffling pond more, the wide about 0.1~0.4m of runner, the depth of water is many at 0.1~0.5m, and its shortcoming is that limit wall periphtic algae number is relatively large, makes algal grown rate monitoring data less than normal, simultaneously because water body top layer and bottom illumination condition difference are little, be difficult to form the wawter bloom phenomenon in the natural water, so critical flow velocity is based upon supposition more and supposes that there is some difference with practical situation in the water body on the basis.
(2) water body mobilization dynamic system is provided with simply.
The power that test is at present adopted mainly contains three kinds: electric mixer, water pump and mechanical wiper mechanism, its shortcoming is that current are difficult to form the stable and uniform fluidised form in the runner, the current disturbance is bigger near water pump or the mechanical drying device, because experimental scale is less than normal, make local difference bigger to whole test result influence.
(3) shortage is to the systematic study of water currents algal grown mechanism.
Present research spininess is to a certain specific body of water, inhibited by test or Model Calculation checking current to algal grown, determine the value or the scope of the best or critical flow velocity, and it is less that flow-shape is influenced mechanism research to algal grown, each result of study exists than big-difference, makes achievement in research be difficult to extensive popularization in actual applications.
The control of testing apparatus and test conditions is one of key link of this test.Common testing apparatus is to adopt microscler circulating water channel at present, pump is set or pushes away the water device at a section, and water body takes out by pump in testing apparatus or mechanical promotion can circulate.Its shortcoming is that water disturbance is excessive near drawing water when adopting pump to take out, and algal grown influence in the process of the test is difficult to determine; A section pushes away water, and water volume flow rate is difficult for evenly in the whole runner.In the past in the testing apparatus, flow dynamic is substantially for a bit, and the automatic adjustment of current can make along the journey flow rate of water flow and reach substantially evenly, and when flow velocity was 1.00m/s in institute of the Nanjing ring section tank, the flow velocity maximum of first section and tail section differed and is 0.10m/s.
In addition, Chinese patent notification number CN1185334C, 2005 day for announcing January 19, name is called " culture apparatus of fine algae ", cultivate that fine algae uses vaulted, taper shape or round shape culture apparatus are disclosed and in culture apparatus, be provided with the gas discharging apparatus that can move.Culture apparatus comprises transparent interior member (semisphere dome, conical perisporium or round shape perisporium), clear outer member and the bottom that is connected two member lower ends, cylindrical opening portion member is arranged on the external member top, and gas lead-through and culture solution discharge member are arranged in the top.Gas discharging apparatus comprises two relative rectangular base plate, bubble guiding piece and discharge nozzle substantially.Its weak point is, complex structure, and wall influence in limit is bigger, and influence is to the detection of algae index in the water body.
Summary of the invention
Technical problem solved by the invention provides a kind of microcystic aeruginosa class research device, makes the process of the test water body along journey flow velocity basically identical.
In order to solve the technical problem of above-mentioned existence, the technical solution used in the present invention is as follows:
A kind of microcystic aeruginosa class research device, comprise tank, motor and lighting system, the profile of described tank is the circular cylinder that opening makes progress, described motor is arranged on the circle centre position of described tank, the output shaft of described motor is provided with vertical supporting beam, and described supporting beam top is provided with the arm beam that is the level of state, and described arm beam is arranged with and pushes away the water sheet, the described water sheet that pushes away stretches in the described tank, and described lighting system is arranged at the tank periphery.
Further: described lighting system comprises illumination support and top board, and described top board is provided with set lights.
Further: the bottom of described tank is provided with heating system.
Further: the annulus width of described tank is at least 0.8m.
Further: described supporting beam and arm beam are orthogonal.
Further: described arm beam is at least two, and described arm beam is along the annulus uniform distribution.
Further: described arm beam and pushing away between the water sheet flexibly connects.
Further: the quantity that pushes away the water sheet under the described arm beam is at least 4.
Further: the described spacing that pushes away the water sheet is reduced outside to inside by circumference, and the described adjustable angle that pushes away the water sheet is put in order.
Compared with prior art, the invention has the beneficial effects as follows:
1, make the process of the test water body along journey flow velocity basically identical, avoided installing local plug-flow in the past, the unsteady shortcoming of fluidised form makes in the runner current even along Cheng Gengjia.
2, runner sufficiently long reduces limit wall influence, makes flow-shape more stable.
Description of drawings
Fig. 1 is the vertical view of microcystic aeruginosa class research device of the present invention;
Fig. 2 is that the A-A of microcystic aeruginosa class research device of the present invention is to cross-sectional view.
Embodiment
It is research object that the present invention influences mechanism with flow dynamic to microcystic aeruginosa, optimize on the basis at literature survey and experimental technique, take small-scale test and model trial, the method that shop experiment combines with outdoor checking, start with from the growth physiological property of microcystic aeruginosa, the flow effect is influenced mechanism to microcystic aeruginosa carry out systematic study, achievement in research is not only improved flow dynamic control wawter bloom and is taken place theoretical, having filled up flow dynamic influences the blank of mechanism research to algal grown, also will be that effectively control eutrophication water wawter bloom takes place, improve water landscape value the gordian technique support is provided.
Below with reference to accompanying drawing and preferred embodiment, technical scheme of the present invention is done detailed description:
As shown in Figure 1 and Figure 2, comprise tank 1, motor 2, supporting beam 3, arm beam 4 and lighting system on the structural entity of microcystic aeruginosa class research device; The profile of tank 1 is the circular cylinder of upper opening, and tank 1 adopts synthetic glass to make, the annulus width of tank 1 usually 〉=0.8m, in this preferred embodiment, tank 1 specifically be of a size of internal diameter 1m, external diameter 2.6m, wide 0.8m, high 1m.The bottom of tank 1 is equipped with heating system 6, and heating system 6 adopts ground heating principle or Electric heating, makes test water body water temperature continue to keep more than 25 ℃; Home position at tank 1 is equipped with a synchronous variable-frequency motor 2, and the output shaft of synchronous frequency conversion motor 2 is provided with vertical supporting beam 3, and supporting beam 3 tops are provided with arm beam 4; Arm beam 4 is more than two, optimal way, and arm beam 4 is 3, and along the annular water tank uniform distribution, arm beam 4 is higher than tank 1, and arm beam 4 is vertical with supporting beam 3; Arm beam 4 be provided with insert tank 1 straight down push away water sheet 5, arm beam 4 and pushing away between the water sheet 5 flexibly connects, the upstream face adjustable angle that pushes away the water sheet is whole, pushes away water sheet 5 and stretches into tank 1 bottom from sink top.In this preferred embodiment, the quantity that pushes away water sheet 5 of arm beam 4 every sides is more than 4.Optimal way, the quantity that pushes away water sheet 5 of arm beam 4 every sides is 14.Push away water sheet 5 and reduced gradually by circumference spacing outside to inside, the adjustable angle that pushes away the water sheet is whole.Lighting system is arranged at tank 1 periphery, comprises illumination support 7 and top board 8.Illumination support 7 is distributed in outside the tank 1, and the top of illumination support 7 is higher than tank 1, and the roof is fixed on the top of illumination support 7.Set lights is distributed on the top board 8, and set lights can warranty test water body top layer intensity of illumination reach 3300lux; The top board 8 of lighting system is a metal construction, can move up and down.Lighting system also can adopt other to be distributed in the structure of tank periphery.In this preferred embodiment, illumination support 7 height are 2.2m, set spacing 2.6m, and set lights is apart from arm beam 0.1m during test.
Among the present invention, must carry out shading treatment in the process of the test.Tank 1 is a circular cylinder, and current circulate in tank 1 equably, satisfies the sufficiently long requirement of runner.Adopt synchronous frequency conversion motor 2 to drive arm fine strains of millet 4 and push away water sheet 5 and rotate, rotate in tank 1 thereby promote water, avoided installing local plug-flow in the past, the unsteady shortcoming of fluidised form makes in the runner current even along Cheng Gengjia.
Because this device be annular, push away water sheet 5 speed of rotation (linear velocity) difference on the arm fine strain of millet 4, the speed of rotation that water sheet 5 speed of rotation and close interior ring push away water sheet 5 that pushes away near outer shroud in the tank differs bigger, and its value is about 50%.So medial and lateral flow rate of water flow difference in the runner.It is poor to adjust the annular channel medial and lateral flow rate of water flow from following two aspects:
Be current self adjustment on the one hand, current are liquid in the runner, and current can automatically adjust in runner, and the even direction of current direction transverse flow speed is developed.On the other hand, adjust the density and the upstream face angle that push away water sheet 5 in the tank, make its ecto-entad tight gradually, the upstream face area ecto-entad that pushes away the water sheet increases gradually, can promote transverse flow speed homogenizing in the tank 1.

Claims (9)

1. microcystic aeruginosa class research device, comprise tank, motor and lighting system, it is characterized in that, the profile of described tank is the circular cylinder that opening makes progress, and described motor is arranged on the circle centre position of described tank, and the output shaft of described motor is provided with vertical supporting beam, described supporting beam top is provided with the arm beam that is the level of state, described arm beam is arranged with and pushes away the water sheet, and the described water sheet that pushes away stretches in the described tank, and described lighting system is arranged at the tank periphery.
2. microcystic aeruginosa class research device as claimed in claim 1 is characterized in that: described lighting system comprises illumination support and top board, and described top board is provided with set lights.
3. microcystic aeruginosa class research device as claimed in claim 1, it is characterized in that: the bottom of described tank is provided with heating system.
4. microcystic aeruginosa class research device as claimed in claim 1 is characterized in that: the annulus width of described tank is at least 0.8m.
5. microcystic aeruginosa class research device as claimed in claim 1 is characterized in that: described supporting beam and arm beam are orthogonal.
6. as claim 1 or 5 described microcystic aeruginosa class research devices, it is characterized in that: described arm beam is at least two, and described arm beam is along the annulus uniform distribution.
7. microcystic aeruginosa class research device as claimed in claim 1 is characterized in that: described arm beam and pushing away between the water sheet flexibly connects.
8. microcystic aeruginosa class research device as claimed in claim 1 is characterized in that: the quantity that pushes away the water sheet under the described arm beam is at least 4.
9. as claim 1 or 8 described microcystic aeruginosa class research devices, it is characterized in that: the described spacing that pushes away the water sheet is reduced outside to inside by circumference, and the described adjustable angle that pushes away the water sheet is put in order.
CN 201110050010 2011-03-02 2011-03-02 Microcystis aeruginosa research device Expired - Fee Related CN102174373B (en)

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CN102174373B CN102174373B (en) 2013-05-22

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103344410A (en) * 2013-06-13 2013-10-09 扬州大学 Simulation device of continuous and stable flowing water body
CN103371098A (en) * 2012-04-18 2013-10-30 中国水利水电科学研究院 Experimental apparatus, system and method for aquatic plants

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348347B1 (en) * 1998-03-31 2002-02-19 Micro Gaia Co., Ltd. Fine algae culture device
CN2898046Y (en) * 2006-03-09 2007-05-09 微聚科技股份有限公司 Agitation wing structure of microbiological culture dish
CN201046966Y (en) * 2007-05-18 2008-04-16 王逢旦 Production device for culturing microalgae
CN201746538U (en) * 2010-08-02 2011-02-16 格特生物制药(天津)有限公司 Compound-bacteria mixed cultivating device
CN202054823U (en) * 2011-03-02 2011-11-30 北京市水利科学研究所 Microcystis-aeruginosa researching device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348347B1 (en) * 1998-03-31 2002-02-19 Micro Gaia Co., Ltd. Fine algae culture device
CN2898046Y (en) * 2006-03-09 2007-05-09 微聚科技股份有限公司 Agitation wing structure of microbiological culture dish
CN201046966Y (en) * 2007-05-18 2008-04-16 王逢旦 Production device for culturing microalgae
CN201746538U (en) * 2010-08-02 2011-02-16 格特生物制药(天津)有限公司 Compound-bacteria mixed cultivating device
CN202054823U (en) * 2011-03-02 2011-11-30 北京市水利科学研究所 Microcystis-aeruginosa researching device

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103371098A (en) * 2012-04-18 2013-10-30 中国水利水电科学研究院 Experimental apparatus, system and method for aquatic plants
CN103371098B (en) * 2012-04-18 2015-07-22 中国水利水电科学研究院 Experimental apparatus, system and method for aquatic plants
CN103344410A (en) * 2013-06-13 2013-10-09 扬州大学 Simulation device of continuous and stable flowing water body
CN103344410B (en) * 2013-06-13 2015-08-19 扬州大学 A kind of analogue means of continous-stable current water

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