CN201673159U - Protein chip cleaning box - Google Patents
Protein chip cleaning box Download PDFInfo
- Publication number
- CN201673159U CN201673159U CN2010201720204U CN201020172020U CN201673159U CN 201673159 U CN201673159 U CN 201673159U CN 2010201720204 U CN2010201720204 U CN 2010201720204U CN 201020172020 U CN201020172020 U CN 201020172020U CN 201673159 U CN201673159 U CN 201673159U
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- box
- lid
- box body
- protein chip
- opening end
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Abstract
The utility model belongs to the biological device technical field and relates to a protein chip cleaning box mainly for cleaning the chip in the chemiluminescence detection method of double-antibody sandwich method, which solves the problems of the prior protein chip cleaning box that the chip can not be substantively cleaned. The protein chip cleaning box comprises a cover body and a box body, wherein a gas pressure balance hole for communicating the inner part of the box body with the outside is arranged on the cover body.
Description
Technical field
The utility model belongs to the biological plant technical field, relates to a kind of protein chip and washes box, and this washes box mainly is to be used in the washing to chip of the chemical luminescence detection method of double antibody sandwich method.
Background technology
Biochip technology is that life science and microelectronics are intersected the new technology that infiltration grows up mutually in recent years.Biochip technology comprises three parts, that is: nucleic acid chip, protein chip and chip lab.Protein chip technology is the high flux protein detection technology platform that designs according to interactional principle between the protein.
The detection principle of the multi-tumor marker protein chip detection system of producing (C-12) is based on the chemical luminescence detection method of double antibody sandwich method, and flow process is as follows:
(1) chip manufacturing: the specific antibody of 12 kinds of tumor markerses is passed through point sample instrument dot matrix specking in accordance with regulations on the NC film, antibody is combined with NC film solid phase carrier, form insolubilized antibody, make protein chip through processes such as BSA sealing, dryings again.
(2) add and examined sample, make it and insolubilized antibody contact reaction a period of time, allow antigen in the sample and the antibodies on the solid phase carrier, form the solid phase antigen compound.Other unconjugated materials are removed in washing.
(3) add enzyme labelled antibody, the antigen on the solid-phase immunity compound is combined with enzyme labelled antibody.Thoroughly wash unconjugated enzyme labelled antibody.The amount positive correlation of examined object matter in enzyme amount that have on the solid phase carrier this moment and the sample.
(4) add substrate: the substrate for enzymatic activity in the sandwich compound makes substrate luminous.Carry out the qualitative or quantitative of this antigen according to light intensity.
(5) detect the light signal that produces through the enzyme-catalyzed change chemical reaction, the line data analysis of going forward side by side with special chip detector.
In testing process, two chip washing steps are arranged.The washing order ground be in the flush away reactant liquor less than with the material of solid phase antigen or antibodies and in course of reaction non-specific adsorption in the interfering material of solid phase carrier.Washing is not reaction in the process of chip detection, but decision experiment key of success.Washing as not thorough will cause following adverse consequences: the first, and the chip blank value is higher, causes high s/n ratio, makes the failure of an experiment.The second, the enzyme labelled antibody that does not have the heterogenetic antigen of combination and do not have combination makes the result inaccurate if do not clean the reliability that will influence test data of chip.
For the effect that guarantees to wash, require:
Wash the good airproof performance of box, do not stay bubble
Wash and to drive bubble away after box adds washing lotion and chip, can not have big slightly bubble to produce in the process of washing, necessarily will get well so wash the sealing of box.
All be in the constant temperature shaking table, to wash chip at every turn.Requirement can not have washing lotion to spill at every turn in shaking the process of washing, otherwise will influence the effect of washing, and because the C-12 chip is that quantitatively (non-qualitative) detects, requires the condition of repeatedly washing to be consistent, and is quite high to the requirement of washing the box sealing therefore.
Wash box certain durability will be arranged
The mode of washing of multi-tumor marker protein chip detection system (C12) is as follows: chip is put into special-purpose chip wash box, add cleansing solution, will wash box then and vertically put into shaking table, shaking table is transferred to 250rpm, 37 ℃ were washed 8 minutes.In whole process, need wash altogether 8 times.Shake at every turn and will take out chip after washing and wash the cleansing solution of using in the box, and with this cleansing solution flushing chip, because each detection will be opened lid 8 times, therefore washing box should there be certain durability.
Yet existing protein chip is washed box such as Fig. 1, Fig. 2, shown in Figure 3, and in use mainly there are following three problems in this box of washing: the first, put into that bubble row does not fall after chip and the washing lotion; The second, lid and box body are poorly sealed, and washing lotion can flow out in washing process; The 3rd, the box body poor durability is easy to cracking.Thereby be difficult to reach the requirement that to satisfy for the clean result that guarantees chip.
The utility model content
Technical problem to be solved in the utility model is to use existing protein chip to wash box protein chip is washed, can not make chip reach the problem of suitable clean result, and provide a kind of protein chip to wash box at this problem, wash box with respect to existing protein chip, protein chip described in the utility model is washed box and has been carried out technically repeatedly improving, make chip reach good clean result thereby can satisfy, should be noted that the box of washing described in the utility model is mainly used in the chemical luminescence detection method of double antibody sandwich method washing to chip.
Specifically, the utility model solution that technical improvement can be successful first time of providing existing wash that box exists put into the problem that bubble row does not fall after chip and the washing lotion:
A kind of protein chip is washed box, comprises lid and box body, it is characterized in that lid is provided with the air equalizer opening that will be in communication with the outside in the box body.
After adopting said structure, bubble can be discharged smoothly by air equalizer opening.
At least on the basis of the technological improvement first time, the utility model provides technical improvement for the second time, existing wash box cover and box body is poorly sealed to solve, the problem that washing lotion can flow out in washing process, address this problem and to use any effective seal technology, for the utility model, mainly adopted two kinds of technical schemes to solve above-mentioned technical matters, first kind of technical scheme is:
The projection that lid comprises pressing plate and is arranged on the pressing plate middle part, the inside surface relative engagement of described projection side and box opening end end, described lid also comprises O-ring seal, the projection side is provided with the groove that snaps in for O-ring seal, and described lid closely cooperates by O-ring seal and box opening end end inside surface at least.
Say as background technology, existing lid and the box body of washing in the box (as Fig. 1 and Fig. 2), only be to completely cut off inner with extraneous being communicated with of box body by the lid projection side and the cooperation of box opening end end inside surface, such technical scheme will make wash box produce poorly sealed, the problem that washing lotion flows out in the box body easily in washing process.For this reason, the lid of washing box described in the utility model also comprises O-ring seal, the projection side is provided with the groove that snaps in for O-ring seal, lid closely cooperates by O-ring seal and box opening end end inside surface at least, thereby with cover cap after on the box body, O-ring seal is in confined state all the time, can guarantee to make the slit that O-ring seal is filled expires between box opening end end inside surface and the lid projection side, guarantees the leakproofness of box body inside.
Another kind of technical scheme is:
The projection that lid comprises pressing plate and is arranged on the pressing plate middle part, lid also comprises sealing gasket, described projection and pressing plate junction are provided with the groove that snaps in for sealing gasket, the described box of washing also comprises the hold-down devices that lid is pressed down towards the box opening end, and described lid closely cooperates by sealing gasket and box opening end port surface at least.
The principle of technique scheme is similar with first kind of technical scheme, effect by hold-down devices, can make the sealing gasket generation deformation of projection and pressing plate junction, make the slit that O-ring seal is filled expires between projection and the pressing plate junction, guarantee the leakproofness of box body inside.
For further improving the technical improvement second time of the present utility model, thereby make the box of washing described in the utility model can remain good sealing effectiveness, perfectly safe, those skilled in the art can also be used in combination above-mentioned two kinds of technical schemes, thereby increase following technical characterictic again on the basis of first kind of technical scheme:
The described box of washing also comprises the hold-down devices that lid is pressed down towards the box opening end, and lid also comprises sealing gasket, and described projection and pressing plate junction are provided with the groove that snaps in for sealing gasket, and described lid closely cooperates by sealing gasket and box opening end port surface at least.
And the technician can also select such hold-down devices further in containing the technical scheme of hold-down devices, to guarantee the good effect that presses down:
Described hold-down devices comprises holder that is fixed on the box body lateral surface and the elastic lock that is provided with jag, holder is provided with socket, elastic lock one end is connected with holder by socket, and pressing plate is provided with the groove that snaps in for jag with the another side that is provided with projection relatively.
First or the basis of technological improvement for the second time on, the utility model also provides technical for the third time improvement, existingly washes the box body poor durability that box exists, the problem that is easy to ftracture with what solve that background technology partly mentions:
The openend end of described box body is the lip limit, and box opening end end inside surface is a lip limit medial surface, and described lip limit is an one-piece construction.
After adopting said structure, just can effectively prevent the cracking of box body side, certainly, after the openend end of box body was the lip limit, lip limit medial surface was box opening end end inside surface, thereby can realize technical for the third time improvement smoothly.Certainly technical for the third time improvement described in the utility model can be used separately, also can combine with technical improvement for the second time, thereby lay the foundation for good sealing effectiveness.
Simultaneously, those skilled in the art also can be further on first, second or technical for the third time improved basis, what solve that background technology partly mentions existingly washes the box body poor durability that box exists, the problem that is easy to ftracture, for example:
Box body is formed by tenon fourth of the twelve Earthly Branches ways of connecting assembly unit bonding by some side plates that constitute the box body side and the base plate that constitutes box bottom.
Description of drawings
Fig. 1 is the structural representation that the described existing protein chip of background technology part is washed the lid of box;
Fig. 2 is the structural representation that the described existing protein chip of background technology part is washed the box body of box;
Fig. 3 is the partial cross section figure that the described existing protein chip of background technology part is washed the box body of box;
Fig. 4 is the structural representation that the described a kind of protein chip of embodiment is washed box;
Fig. 5 is the lid perspective view that the described a kind of protein chip of embodiment is washed box;
Fig. 6 is the lid side structure synoptic diagram that the described a kind of protein chip of embodiment is washed box;
Fig. 7 is the lid vertical view that the described a kind of protein chip of embodiment is washed box;
Fig. 8 is the box body structure synoptic diagram that the described a kind of protein chip of embodiment is washed box;
Fig. 9 is the sectional view that the described a kind of protein chip of embodiment is washed box plate and side plate junction in the box.
Embodiment
In order to make those skilled in the art can deepen understanding of the present utility model is described in detail the technical solution of the utility model below by embodiment.
Embodiment
A kind of protein chip is washed box, comprises lid and box body, and lid is provided with the air equalizer opening that will be in communication with the outside in the box body, thereby bubble can be discharged smoothly by air equalizer opening.
In order to solve the existing box body poor durability that box exists of washing, be easy to ftracture and existing wash that box exists put into the problem that bubble row does not fall after chip and the washing lotion, present embodiment is respectively with reference to the technical for the third time improvement of this instructions utility model content part, also promptly:
Described box opening end end is the lip limit, and box opening end end inside surface is a lip limit medial surface, and described lip limit is an one-piece construction.
Simultaneously, present embodiment is abandoned the existing production method of washing box: i.e. the side of box body and underrun bonding is made, and so easy integral body of box of washing ftractures.For this reason, the described box body of present embodiment is formed by tenon fourth of the twelve Earthly Branches ways of connecting assembly unit bonding by some side plates that constitute the box body side and the base plate that constitutes box bottom.
Existing wash box cover in order to solve with box body is poorly sealed, the problem that washing lotion can flow out in washing process, the means of present embodiment employing are that first kind of technical scheme will introducing in the utility model content part is used in combination with second kind of technical scheme, also promptly:
Lid comprises pressing plate, is arranged on the projection and the O-ring seal at pressing plate middle part, the inside surface relative engagement of described projection side and box opening end end, the projection side is provided with the groove that snaps in for O-ring seal, and O-ring seal have at least the part from groove to exposing outside, so just can make O-ring seal insert groove comparatively closely, lid is closely cooperated by O-ring seal and box opening end end inside surface at least.Thereby make the slit that O-ring seal is filled expires between box opening end end inside surface and the lid projection side, to guarantee the leakproofness of box body inside.
The described box of washing also comprises the hold-down devices that lid is pressed down towards the box opening end, and lid also comprises sealing gasket, and described projection and pressing plate junction are provided with the groove that snaps in for sealing gasket, and described lid closely cooperates by sealing gasket and box opening end port surface at least.
Why adopt two kinds of technical schemes that combine, be because in the use of reality, the technician can notice, there are the plural at least face that is in contact with one another in the junction of lid center platen and projection and box opening end port, therefore more be easy to generate the slit than box opening end end inside surface and contact position, projection side, can guarantee the leakproofness of box body inside though adopt first kind of technical scheme, but for the purpose of safe, the technician can be provided with the groove that snaps in for sealing gasket at projection and pressing plate junction, equally, sealing gasket have at least part from groove to exposing outside, so that lid is closely contacted by sealing gasket and box opening end port are surperficial at least.
Certainly, the groove that sealing gasket is only arranged and supply sealing gasket to snap in is not enough, and its reason is that sealing gasket does not produce deformation, this just causes sealing gasket can not fill the junction of full pressing plate and projection and the slit that box opening end port exists, and the sealing function of sealing gasket is had a greatly reduced quality.For this reason, the technician can be provided with a hold-down devices that lid is pressed down towards the box opening end washing on the box, the purpose of this hold-down devices not only with lid is pressed down towards the box opening end, be also to guarantee that lid can be firm covers on box body, thereby can not be washed the influence that box waves.
The hold-down devices that realizes above-mentioned purpose has a lot, for example, magnet that can attract each other in lid and box body setting or the like, the technician can be from different angles (as manufacturing cost, operation ease, portable or the like) consider, select suitable hold-down devices, as a kind of reference, the described hold-down devices of present embodiment is a kind of snap close, be that described hold-down devices comprises holder that is fixed on cartridge outer surface and the elastic lock that is provided with jag, holder is provided with socket, elastic lock one end is connected with holder by socket, and pressing plate is provided with the groove that snaps in for jag with the another side that is provided with projection relatively.
Following present embodiment further show again have an above-mentioned technical characterictic wash the box material object:
A kind of protein chip is washed box, as Fig. 4, Fig. 5, Fig. 6, Fig. 7, Fig. 8 and shown in Figure 9, is made up of lid 21 and box body 22.Lid 21 is the integral structure that comprises pressing plate 55, is arranged on the projection 54 at pressing plate 55 middle parts, lid 21 has air equalizer opening 31, prevent the groove 34 of elastic wire snap close landing (the elastic wire snap close is snapped in), projection 54 is provided with the groove 32 of placing O-ring seal and the groove 33 of placing sealing gasket.Box body 22 is a splicing construction, constitute by the holder 56 that jack is connected with elastic wire snap close 45 respectively by long lumbar hole structure lip limit, upper end 41, lip limit medial surface 53 (being box opening end end inside surface), four blocks of side plates 42, base plate 43 and 6 screens pieces 44, two elastic wire snap closes 45 and two, adopt the tenon fourth of the twelve Earthly Branches to be connected 51 mode between side sheet room and side plate and base plate and connect.
Box body 22 is the organic glass material, connects 51 bonding the forming of mode by the polylith parts of machine finishing moulding by falcon, and physical dimension is long 90mm, wide 30mm, high 110.5mm.Mainly form: the holder 56 that lip limit 41, lip limit medial surface 53 (being box opening end end inside surface), four blocks of side plates 42,43,6 screens piece of base plate 44, two elastic wire snap closes 45 and two are connected by jack with elastic wire snap close 45 respectively by following components.The difference in height of upper lip 41 and lower lip 52 is 8mm.The inside long lumbar hole on lip limit 41 is of a size of two ends semicircle internal diameter φ 22mm, and the center of circle spacing of two semicircles is 62mm.Such lip limit structure both can prevent box body 22 cracking, can guarantee again that protein chip (C12) is put into smoothly to wash box.Adopt the tenon fourth of the twelve Earthly Branches to be connected 51 mode between side sheet room and side plate and base plate, make splice more firm.Elastic wire snap close 45 structures make the better tightness of washing box.
Claims (10)
1. a protein chip is washed box, comprises lid and box body, it is characterized in that lid is provided with the air equalizer opening that will be in communication with the outside in the box body.
2. a kind of protein chip according to claim 1 is washed box, it is characterized in that the projection that lid comprises pressing plate and is arranged on the pressing plate middle part, the inside surface relative engagement of described projection side and box opening end end, described lid also comprises O-ring seal, the projection side is provided with the groove that snaps in for O-ring seal, and described lid closely cooperates by O-ring seal and box opening end end inside surface at least.
3. a kind of protein chip according to claim 1 is washed box, it is characterized in that the described box of washing also comprises the hold-down devices that lid is pressed down towards the box opening end, lid also comprises sealing gasket, described projection and pressing plate junction are provided with the groove that snaps in for sealing gasket, and described lid closely cooperates by sealing gasket and box opening end port surface at least.
4. a kind of protein chip according to claim 2 is washed box, it is characterized in that the projection that lid comprises pressing plate and is arranged on the pressing plate middle part, lid also comprises sealing gasket, described projection and pressing plate junction are provided with the groove that snaps in for sealing gasket, the described box of washing also comprises the hold-down devices that lid is pressed down towards the box opening end, and described lid closely cooperates by sealing gasket and box opening end port surface at least.
5. wash box according to claim 3 or 4 described a kind of protein chips, it is characterized in that described hold-down devices comprises holder that is fixed on the box body lateral surface and the elastic lock that is provided with jag, holder is provided with socket, elastic lock one end is connected with holder by socket, and pressing plate is provided with the groove that snaps in for jag with the another side that is provided with projection relatively.
According to claim 12 or 34 or described a kind of protein chip wash box, the openend end that it is characterized in that described box body is the lip limit, box opening end end inside surface is a lip limit medial surface, described lip limit is an one-piece construction.
7. a kind of protein chip according to claim 5 is washed box, and the openend end that it is characterized in that described box body is the lip limit, and box opening end end inside surface is a lip limit medial surface, and described lip limit is an one-piece construction.
8. wash box according to claim 1 or 2 or 3 or 4 described a kind of protein chips, it is characterized in that box body is formed by tenon fourth of the twelve Earthly Branches ways of connecting assembly unit bonding by some side plates that constitute the box body side and the base plate that constitutes box bottom.
9. a kind of protein chip according to claim 5 is washed box, it is characterized in that box body is formed by tenon fourth of the twelve Earthly Branches ways of connecting assembly unit bonding by some side plates that constitute the box body side and the base plate that constitutes box bottom.
10. a kind of protein chip according to claim 6 is washed box, it is characterized in that box body is formed by tenon fourth of the twelve Earthly Branches ways of connecting assembly unit bonding by some side plates that constitute the box body side and the base plate that constitutes box bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201720204U CN201673159U (en) | 2010-04-27 | 2010-04-27 | Protein chip cleaning box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201720204U CN201673159U (en) | 2010-04-27 | 2010-04-27 | Protein chip cleaning box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201673159U true CN201673159U (en) | 2010-12-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201720204U Expired - Lifetime CN201673159U (en) | 2010-04-27 | 2010-04-27 | Protein chip cleaning box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201673159U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102756217A (en) * | 2012-07-10 | 2012-10-31 | 新疆雪峰科技(集团)股份有限公司 | Electronic detonator wire laser welding clamp and using method thereof |
-
2010
- 2010-04-27 CN CN2010201720204U patent/CN201673159U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102756217A (en) * | 2012-07-10 | 2012-10-31 | 新疆雪峰科技(集团)股份有限公司 | Electronic detonator wire laser welding clamp and using method thereof |
| CN102756217B (en) * | 2012-07-10 | 2014-11-26 | 新疆雪峰科技(集团)股份有限公司 | Electronic detonator wire laser welding clamp and using method thereof |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20101215 |