CN205861686U - A kind of biochip reaction device - Google Patents
A kind of biochip reaction device Download PDFInfo
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- CN205861686U CN205861686U CN201620014943.4U CN201620014943U CN205861686U CN 205861686 U CN205861686 U CN 205861686U CN 201620014943 U CN201620014943 U CN 201620014943U CN 205861686 U CN205861686 U CN 205861686U
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- negative pressure
- reaction
- membrane matrix
- biochip
- reaction device
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Abstract
The utility model discloses a kind of biochip reaction device.This biochip reaction device, including the reaction vessel being made up of top cover, membrane matrix, support liner and bottom, it is characterised in that described reaction unit also includes that negative pressure device, described negative pressure device are connected by bottom；And top cover and bottom seal and coincide, and sandwich supports liner and membrane matrix.This device uses negative pressure mode, is greatly accelerated the efficiency of traditional biological reaction (enzyme exempts from reaction or nucleic acid hybridization reaction), improves the sensitivity of detection, shorten the response time.
Description
Technical field
This utility model relates to technical field of biological, the particularly inspection-free survey of enzyme, chemiluminescence detection or protein chip,
Genechip detection field.
Background technology
Immunoreation or nucleic acid hybridization reaction are all by probe (antigen, antibody or nucleic acid) is fixed on stromal surface
(ELISA Plate, microscope slide or cellulose membrane), is subsequently adding biological specimen (serum, urine, saliva or other human body fluid), by visiting
Material to be checked in pin capture biological specimen.Traditional reaction unit, is all to join in reaction vessel by biological specimen, allows probe
Combine with biological specimen random collision.The efficiency that this collision combines is the lowest, and reaction will be through (3 hours for a long time
Above) can be only achieved balance.Reach the speed of balance to add fast response, conventional method is concussion, but still needs 2 hours
The above response time.Sometimes for shortening the response time, detection sensitivity can only be sacrificed, be just also not reaching to poised state
Starting washing, and the diversity of clinical sample is very big, it is different that different samples reach to balance the required time, therefore uses identical
Response time obviously can bring error into.And gold colloidal detection is the absorbability utilizing nitrocellulose filter, sample is allowed to pass through it
The antibody of fixed position, surface, is combined reaction, reaches the effect quickly detected, but due to the water absorbing capacity of nitrocellulose filter
Power is limited, it is impossible to wash film, and therefore detection background can be the highest, and sensitivity is low.
Summary of the invention
In order to overcome the problems referred to above (reaction reach balance time length or detection background high sensitivity low), this utility model
Traditional reaction unit is improved, it is provided that a kind of high-performance bio chip reaction unit.This device uses pressure allow biology
Sample, quickly through membrane matrix surface, sufficiently contacts with the probe being fixed on membrane matrix surface, and reaches quick and sufficient
Reaction.
This utility model provides a kind of biochip reaction device, including by top cover, membrane matrix, support liner and bottom group
The reaction vessel become, described reaction unit also includes that negative pressure device, described negative pressure device are connected by bottom；And push up lid and bottom
Lid seals and coincide, and sandwich supports liner and membrane matrix.
Preferably, the top of described top cover is elongated graded tube.
Preferably, described graded tube is internal fills glass.The biological specimen sucked is carried out adsorption filtration；Also may be used on glass
With adsorption antigen, antibody, nucleic acid or other probe, can quickly mix with the biological specimen added or react.
Preferably, described negative pressure device is Manual medical syringe or Electric negative pressure device.
Preferably, described membrane matrix is nitrocellulose filter or nylon membrane, porose on described membrane matrix；On described membrane matrix
Also incorporating probe, described probe is protein, nucleic acid, polysaccharide or micromolecular compound.
Preferably, described support liner is loose permeable hard holder.
Additionally, the biological specimen that described device can detect can be serum, urine, saliva or other human body fluid.
The utility model has the advantage of, utilize negative pressure device, accelerate the biological specimen in reaction vessel through densification
Membrane matrix, and fully react with combining probe thereon, thus shorten the response time, improve detection sensitivity.With prior art
Comparing, the beneficial effects of the utility model are: 1, apparatus structure is succinct, easy to use.2, the anti-of biological specimen and probe is improved
Answer efficiency, shorten biological specimen and the response time of probe, raising detection sensitivity.3, reacted waste liquid is directly entered injection
In device or negtive-pressure implement, prevent the harm to operator of the harmful organism sample and the pollution to environment.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Wherein, 1 scale, 2 glass fillers, 3 top covers, 4 membrane matrixs, 5 support liner, 6 bottoms, 7 negative pressure devices.
Fig. 2 is scanning result after two different operatings.Wherein, a left side is the height of biochip reaction device of the present utility model
The result of validity response operator scheme, the right side is the result of the traditional detection method of matched group protein chip.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, structure of the present utility model made detailed explanation and explanation, but be not to this
The restriction of utility model structure.
As it is shown in figure 1, this device comprises reaction vessel and negative pressure device 7.Reaction vessel by graded tube 1, glass filler 2,
Top cover 3, membrane matrix 4, support liner 5 and bottom 6 are constituted.Negative pressure device 7 is negtive-pressure implement (electronic).Top cover 3 and bottom 6 seal kiss
Closing, sandwich supports liner 5 and film base 4 matter.
Utilizing the biochip reaction device shown in Fig. 1 can carry out protein chip quickly to detect, its operating procedure is as follows:
1. prepared by chip, by probe (antibody) point (cellulose nitrate of the described preferred aperture 8um of membrane matrix 4 on membrane matrix 4
Element film), constitute antibody chip microarray.Then membrane matrix 4 is placed on support liner 5.
2. suck a certain amount of biological specimen (serum) by negative pressure device 7 and arrive in graded tube 1, by vacuum cavitations, instead
After answering 1 ~ 10 minute, biological specimen is completely through chip film substrate；
3. suck a certain amount of washing liquid (preferably PBST washing liquid), by vacuum cavitations, after 1 ~ 10 minute by negative pressure device 7
Washing liquid is fully washed completely through membrane matrix 4；
4. suck a certain amount of detection antibody by negative pressure device 7, by vacuum cavitations, all inspections after reacting 1 ~ 10 minute
Survey antibody penetration membrane matrix 4 fully to react；
5. sucking a certain amount of washing liquid by negative pressure device 7, by vacuum cavitations, after 1 ~ 10 minute, washing liquid is completely through film
Substrate 4 is fully washed；
6. sucking a certain amount of fluorescent dye by negative pressure device 7, by vacuum cavitations, after 1 ~ 10 minute, dyestuff is the most saturating
Cross membrane matrix；
7. suck a certain amount of washing liquid (preferably PBST washing liquid), by vacuum cavitations, after 1 ~ 10 minute by negative pressure device 7
Washing liquid is fully washed completely through chip film substrate, is then scanned.
The traditional detection method of matched group protein chip is that biological specimen (can be serum or urine etc.) is joined core
In sheet reacting hole, constant-temperature incubation 2 hours, rear employing is washed panel assembly and is repeatedly washed and pat dry, and adds detection antibody incubation 2
Individual hour, after the most repeatedly wash plate and pat dry, finally add fluorescent dye and carry out reacting 1 hour, after the most repeatedly wash plate and pat dry.?
After under imaging device, carry out imaging.Whole process probably needs about 7 hours.
Fig. 2 is scanning result after two different operatings, and wherein, left figure is biochip reaction device of the present utility model
The result of highly effective reaction operator scheme, right figure is the result of the traditional detection method of matched group protein chip.It is found that use
Biochip reaction device testing result signal of the present utility model is higher, and background is lower, substantially increases detection sensitivity.
It addition, time-consuming 6 ~ 60 minutes of the highly effective reaction operator scheme of biochip reaction device of the present utility model, and egg
Time-consuming about 7 hours of the detection method that chip is traditional in vain, result issuing time is greatly shortened.Because biology of the present utility model
Chip reaction unit uses negative pressure mode, allows the biological specimen added in graded tube 1 through glass filler 2 and fine and close membrane matrix
4, and the probe with combined thereon fully reacts, and cannot in conjunction with material taken away by quick wash along with solvent, enter
Enter in the negative pressure device under it.This biochip reaction device is greatly accelerated traditional biological reaction, and (enzyme exempts from reaction or nucleic acid is miscellaneous
Hand over reaction) efficiency, improve the sensitivity of detection, shorten the response time.
The foregoing is only preferred embodiment of the present utility model, not thereby limit the scope of the claims of the present utility model,
Equivalent structure or equivalence flow process that every accompanying drawing content utilizing this utility model description is made convert, or directly or indirectly transport
It is used in other relevant technical fields, is the most in like manner included in scope of patent protection of the present utility model.
Claims (6)
1. a biochip reaction device, including the reaction vessel being made up of top cover, membrane matrix, support liner and bottom, its
Being characterised by, described reaction unit also includes that negative pressure device, described negative pressure device are connected by bottom；And top cover and bottom are close
Envelope is coincide, and sandwich supports liner and membrane matrix.
2. biochip reaction device as claimed in claim 1, it is characterised in that the top of described top cover is elongated scale
Pipe.
3. biochip reaction device as claimed in claim 2, it is characterised in that described graded tube is internal fills glass.
4. the biochip reaction device as described in one of claims 1 to 3, it is characterised in that described negative pressure device is manual
Injector for medical purpose or Electric negative pressure device.
5. the biochip reaction device as described in one of claims 1 to 3, it is characterised in that described membrane matrix is that nitric acid is fine
Dimension element film or nylon membrane, porose on described membrane matrix；Also incorporating probe on described membrane matrix, described probe is protein, core
Acid, polysaccharide or micromolecular compound.
6. the biochip reaction device as described in one of claims 1 to 3, it is characterised in that described support liner is loose
Permeable hard holder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620014943.4U CN205861686U (en) | 2016-01-08 | 2016-01-08 | A kind of biochip reaction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620014943.4U CN205861686U (en) | 2016-01-08 | 2016-01-08 | A kind of biochip reaction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205861686U true CN205861686U (en) | 2017-01-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620014943.4U Active CN205861686U (en) | 2016-01-08 | 2016-01-08 | A kind of biochip reaction device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI778310B (en) * | 2018-12-07 | 2022-09-21 | 南韓商Ｌｇ化學股份有限公司 | Device of manufacturing for chip of diagnosing allergy |
-
2016
- 2016-01-08 CN CN201620014943.4U patent/CN205861686U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI778310B (en) * | 2018-12-07 | 2022-09-21 | 南韓商Ｌｇ化學股份有限公司 | Device of manufacturing for chip of diagnosing allergy |
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