CN1995323A - Blend fermentation method of Callicarpa dichotoma (Lour.) K.Koch. and edible fungus, product therefrom and its uses - Google Patents
Blend fermentation method of Callicarpa dichotoma (Lour.) K.Koch. and edible fungus, product therefrom and its uses Download PDFInfo
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- CN1995323A CN1995323A CNA2007100626547A CN200710062654A CN1995323A CN 1995323 A CN1995323 A CN 1995323A CN A2007100626547 A CNA2007100626547 A CN A2007100626547A CN 200710062654 A CN200710062654 A CN 200710062654A CN 1995323 A CN1995323 A CN 1995323A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A61P1/08—Drugs for disorders of the alimentary tract or the digestive system for nausea, cinetosis or vertigo; Antiemetics
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- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
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Abstract
The invention discloses a composite fermenting method and production and application of white fruit and edible mushroom, which is characterized by the following: deactivating the white fruit leaching liquid and water and auxiliary material under normal temperature and normal pressure; fermenting with edible mushroom mother seed.
Description
Technical field
The present invention relates to the food fermentation field, particularly relate to the mixed fermentation method of Callicarpa dichotoma (Lour.) K.Koch and edible fungus and the goods of acquisition.
Background technology
Callicarpa dichotoma (Lour.) K.Koch, its different sites can be treated various disease, effect is obvious: its skin, rhizome, it is oral after tap water boils leaching, in mastopathy not under the situation of ulcer, anti-inflammatory fast, pain relieving, detumescence, usually produce effects after 24 hours, if mastopathy ulcer, its flower is mashed external application, begin hemostasis, pain relieving, anti-inflammatory, detumescence after 12 hours, the meat of its root partly, and is oral after tap water boils leaching, and treatment cerebral concussion and its sequela also have produce effects, after oral concoction, can cure dizziness, headache and preventing or arresting vomiting in 72 hours.
Callicarpa dichotoma (Lour.) K.Koch is a kind of wild plant, its resistance is very weak, meet the invasion and attack of Chemicals residues such as agricultural chemicals, chemical fertilizer, weedicide, be easy to that mashed root loses vigor and withered, so artificial growth is not easy results very much, output seldom mostly is wild digging and adopts, but unconfined manually digging adopted, and limited resources will face exhaustion.
Summary of the invention
At the defective in the above-mentioned field, the invention provides the method for a kind of Callicarpa dichotoma (Lour.) K.Koch and edible fungus mixed fermentation, can make the Callicarpa dichotoma (Lour.) K.Koch material be enlarged utilization, effectively preserve the ecological environment.
Goods and application thereof that the present invention also provides this method to obtain.
The method of Callicarpa dichotoma (Lour.) K.Koch and edible fungus mixed fermentation is characterized in that the Callicarpa dichotoma (Lour.) K.Koch leach liquor is added behind water and the auxiliary material in the normal temperature and pressure deactivation, plants fermentation with edible fungus is female then.
Described fermenting process comprises the steps: that the water and the auxiliary material that add 20-25 times of volume in (1) Callicarpa dichotoma (Lour.) K.Koch leach liquor boil, and removes slag and gets juice, and (2) add fermentor tank with juice, water vapor deactivation under the normal pressure, be cooled to 26 ℃ ± 2 ℃, (3) inject parent edible fungus kind, submerged fermentation in fermentor tank.
Described fermentor tank is that the patent No. is 03270560.3, and name is called the fermentor tank of putting down in writing in the utility model patent of " cover of edible fungi liquid strain culture tank and culture tank ".
Patent No. 200320126960.X is adopted in the injection of described parent edible fungus kind, and name is called the syringe of putting down in writing in the utility model patent of " edible fungus liquid injection rod and comprise the syringe of this injection rod ".
The described submerged fermentation time is 60-72 hour.Effective solid contents 〉=70g/L. when described submerged fermentation stops
Described Callicarpa dichotoma (Lour.) K.Koch leach liquor is the leach liquor of Callicarpa dichotoma (Lour.) K.Koch skin, stem and/or root, and edible mushrooms is the female kind of black fungus.
The goods that aforesaid method obtains are treated application in the mastotic medicine of ulcer not in preparation.
Described Callicarpa dichotoma (Lour.) K.Koch leach liquor is the leach liquor of Callicarpa dichotoma (Lour.) K.Koch flower, and edible mushrooms is the female kind of black fungus.
The goods that aforesaid method obtains are treated application in the mastotic medicine of ulcer in preparation.
Described Callicarpa dichotoma (Lour.) K.Koch leach liquor is the leach liquor of Callicarpa dichotoma (Lour.) K.Koch root, and edible mushrooms is the female kind of Cordyceps militaris (L.) Link..
The application of the goods that aforesaid method obtains in the medicine of preparation treatment cerebral concussion and sequela thereof.
Deactivation at normal temperatures and pressures after the present invention adds the Callicarpa dichotoma (Lour.) K.Koch leach liquor water and enlarges; the effective constituent that can keep Callicarpa dichotoma (Lour.) K.Koch fully; " saturation steam pressure-less boiler " (patent No.: 200320126611.8) that the inactivating device of normal temperature and pressure can adopt the inventor to invent; be about to fermentor tank and directly push deactivation in the above-mentioned boiler; then edible fungus is injected fermentor tank and carry out mixed fermentation; polysaccharide in Callicarpa dichotoma (Lour.) K.Koch and the edible mushrooms is interacted; thereby the goods that make acquisition have the identical effect of pure Callicarpa dichotoma (Lour.) K.Koch leach liquor; reduced use, made ecotope be able to effective protection the Callicarpa dichotoma (Lour.) K.Koch plant resources.
Fermentation time is preferably 60-72 hour, can guarantee fermentation fully, effective solid contents height, and the polysaccharide content height, the goods therapeutic efficiency of its acquisition is best, and the fermentation termination reaches more than the 70g/L with effective solid contents and is as the criterion.
Edible fungus commonly used has black fungus and Cordyceps militaris (L.) Link..Black fungus is rich in irony, and haemostatic effect is obvious; The pupa worm has been rich in various cordycepins, amino acid and polysaccharide, can improve body immunity.With black fungus or female kind of Cordyceps militaris (L.) Link. and the mixed fermentation of Callicarpa dichotoma (Lour.) K.Koch leach liquor, utilize the fermentative action of its bacterial classification, raw material is enlarged, simultaneously in conjunction with the food therapy effect of above-mentioned two kinds of edible funguses, obtain having the preparation of medicinal efficacy.
Filtering fermentation liquor with the female leach liquor submerged fermentation of planting with Callicarpa dichotoma (Lour.) K.Koch skin, root, stem of black fungus obtains can be prepared into capsule after the filter residue and drying, but can becomes oral liquid or the filtrate condensing crystal is prepared into oral solid formulation after the filtrate deactivation.The preparation that aforesaid method obtains can be used for that ulcer is mastotic not oral, and 2 times on the 1st, can anti-inflammatory fast in 24 hours, end swollen, detumescence, effect is remarkable.
The filtering fermentation liquor that the leach liquor submerged fermentation that female kind of black fungus and Callicarpa dichotoma (Lour.) K.Koch are spent obtains can be prepared into pulvis after the filter residue and drying, and condensing crystal also can be prepared into pulvis after the filtrate deactivation.The pulvis that aforesaid method obtains can be used for the mastotic external application of ulcer, and 1 time on the 1st, can hemostasis in 12 hours, anti-inflammatory, pain relieving, detumescence.
Filtering fermentation liquor with the female leach liquor submerged fermentation of planting with the Callicarpa dichotoma (Lour.) K.Koch fleshy root of Cordyceps militaris (L.) Link. obtains can be prepared into capsule after the filter residue and drying, but can becomes oral liquid or the filtrate condensing crystal is prepared into oral solid formulation after the filtrate deactivation.The preparation that aforesaid method obtains can be used for the oral of cerebral concussion and sequela thereof, and 3 times on the 1st, can in 72 hours, cure dizziness, headache and preventing or arresting vomiting, effect is obvious.
All kinds of preparations that utilize above-mentioned fermentation process to make are identical with the medicinal efficacy of pure Callicarpa dichotoma (Lour.) K.Koch, effectively protected limited Callicarpa dichotoma (Lour.) K.Koch plant resources, reduced to dig and adopted, and the Callicarpa dichotoma (Lour.) K.Koch Biological resources are recovered, and adjust, and protected ecotope.
Embodiment
Material is prepared:
Get 1Kg Callicarpa dichotoma (Lour.) K.Koch skin, root, stem and added the 20-25Kg water boil 50 minutes, obtain leach liquor 1.
Get 1Kg Callicarpa dichotoma (Lour.) K.Koch flower and added the 20-15Kg water boil 50 minutes, obtain leach liquor 2.
After the peeling of Callicarpa dichotoma (Lour.) K.Koch root, get its meat 1Kg and added the 20-25Kg water boil 50 minutes, obtain leach liquor 3.
The Cordyceps militaris (L.) Link. of black fungus is female to be planted: adopt the spore separation method, after cultivated on inclined-plane, plane, microscopy was kept at 4 ℃, preserves it and is no more than 90 days.
Embodiment 1
The tap water of 20-25 times of volume of adding and other conventional auxiliary materials (500g bean powder, 500g Semen Maydis powder, 2Kg white sugar, dipotassium hydrogen phosphate 100g, potassium primary phosphate 100g, sal epsom 50g) azeotropic are 50 minutes in the leach liquor 1, add auxiliary material fermentation density is increased.Remove slag and get juice and directly pour in the 200L fermentor tank, it is 03270560.3 that fermentor tank preferably adopts the patent No., name is called the fermentor tank of putting down in writing in the utility model patent of " cover of edible fungi liquid strain culture tank and culture tank ", must stay 15% space in jar, and inject an amount of foam-reducing or defoamer, the locking blind flange, (patent No.: 200320126611.8) interior deactivation is 12 hours to push " saturation steam pressure-less boiler ", when being cooled to 26 ℃, use " edible fungus liquid injection rod and comprise the syringe of this injection rod " (patent No.: 200320126960.X), the female kind of purpose bacterium black fungus injected " cover of edible fungi liquid strain culture tank and culture tank " interior submerged fermentation, 60 hours, jar is put in detectable level back up to standard (referring to that sugared content is up to standard), filter, encapsulated after the solids drying, can becomes oral liquid or condensing crystal after the filtrate deactivation, makes oral solid formulation.
Detect: dense sugared content 〉=90% in the filtrate concentrates purity of polysaccharide 〉=70% in the crystal of back.
Above-mentioned oral preparations, 2 times on the one, give not that ulcer mastopathy patient takes, anti-inflammatory fast in 24 hours, end swollen, detumescence.
Experimental example:
It is as follows that embodiment 1 gained oral preparations is used curative effect:
Effect was as follows after mastopathy patient's 10 examples were taken medicine of the present invention: all patient's clinical manifestations are bimastism or single breast inflammation, redness, pain, take medicine of the present invention and about 24 hours, begin detumescence, the pain shape alleviates gradually until normally, can fully recover in three days-five days, to no effect not obvious person, total effective rate reaches 100%.
Typical case:
Wang Gui English woman gives birth to nineteen forty-one, lives Huanggang City, Hubei Province Huangzhou District experimental primary school, the teacher, and the hair-cream adenopathy, bimastism inflammation, redness, pain, clothes begin detumescence behind the embodiment gained oral preparations down after 24 hours, and the pain shape alleviates gradually until normally.
Embodiment 2
The tap water that adds 20-25 times of volume in the leach liquor 2, with other conventional auxiliary material (500g rice brans, 500g wheat bran, the 500g sorghum flour, 2Kg white sugar, dipotassium hydrogen phosphate 100g, potassium primary phosphate 100g, sal epsom 50g) azeotropic is 50 minutes, remove slag and get juice and directly pour in the 200L fermentor tank, it is 03270560.3 that fermentor tank preferably adopts the patent No., name is called the fermentor tank of putting down in writing in the utility model patent of " cover of edible fungi liquid strain culture tank and culture tank ", must stay 15% space in jar, and inject an amount of foam-reducing or defoamer, the locking blind flange, (patent No.: 200320126611.8) interior deactivation is 12 hours to push " saturation steam pressure-less boiler ", when being cooled to 28 ℃, use " edible fungus liquid injection rod and comprise the syringe of this injection rod " (patent No.: 200320126960.X), with purpose bacterium black fungus female plant inject " cover of edible fungi liquid strain culture tank and culture tank " (patent No.: 03270560.3) interior submerged fermentation, 65 hours, detectable level afterwards (referring to that sugared content is up to standard) up to standard was put jar, filter, make externally-applied powder after the solids drying, condensing crystal after the filtrate deactivation is ground into pulvis.
Detect: dense sugared content 93% in the filtrate concentrates purity of polysaccharide 75% in the crystal of back.
Above-mentioned pulvis external application 1 time on the one, is given ulcer mastopathy patient external application, quick-acting haemostatic powder anti-inflammatory, pain relieving, detumescence in 12 hours.
Experimental example:
It is as follows that embodiment 2 gained pulvis are used curative effect:
Mastopathy patient's 5 examples: patient's clinical manifestation is bimastism or single breast inflammation, redness and ulcer takes place.Outer application medicine of the present invention began detumescence after 12 hours, erosion is organized gradually and reduced, and necrotic tissue comes off, newborn healthy tissues, recovery from illness in five days to seven days.To no effect not obvious person, total effective rate reaches 100%.
Embodiment 3
The tap water that adds 20-25 times of volume in the leach liquor 3, with other conventional auxiliary material (500g rice brans, the 500g Semen Maydis powder, 3Kg white sugar, dipotassium hydrogen phosphate 100g, potassium primary phosphate 100g, sal epsom 50g) azeotropic is 50 minutes, remove slag and get juice and directly pour in the 200L fermentor tank, must stay 15% space in jar, and inject an amount of foam-reducing or defoamer, and the locking blind flange, (patent No.: 200320126611.8) interior deactivation is 12 hours to push " saturation steam pressure-less boiler ", when being cooled to 24 ℃, (patent No.: 200320126960.X) the female kind of purpose bacterium Cordyceps militaris (L.) Link. injected submerged fermentation in the fermentor tank, 72 hours, jar was put in detectable level back up to standard (referring to that sugared content is up to standard) to use " edible fungus liquid injection rod and comprise the syringe of this injection rod ", filter, encapsulated after the solids drying, can becomes oral liquid or condensing crystal after the filtrate deactivation, makes oral solid formulation.
Detect: dense sugared content 95% in the filtrate concentrates purity of polysaccharide 80% in the crystal of back.
Above-mentioned oral preparations, 3 times on the one, take for cerebral concussion and sequela patient thereof, can in 72 hours, cure dizziness, headache and preventing or arresting vomiting.
Experimental example
It is as follows that embodiment 3 gained oral preparations are used curative effect:
Cerebral concussion patient's 8 examples: patient's clinical manifestation is headache, dizziness, vomiting, takes medicine of the present invention and began sx after 72 hours, and vomiting stops, recovery from illness in five to seven days.To no effect not obvious person, total effective rate reaches 100%.
Typical case:
Patient Weng Bin, 47 years old, people from Huang gang, Hubei, cerebral concussion sequela is taken recovery from illness in seven days behind the present embodiment medicine for many years.
Tong Zhiyong, 33 years old, people from Huang gang, Hubei, cerebral concussion is taken recovery from illness in seven days behind the present embodiment medicine for many years.
Claims (13)
1, the method for Callicarpa dichotoma (Lour.) K.Koch and edible fungus mixed fermentation is characterized in that the Callicarpa dichotoma (Lour.) K.Koch leach liquor is added behind water and the auxiliary material in the normal temperature and pressure deactivation, plants fermentation with edible fungus is female then.
2, the described method of claim 1, the described water that adds is meant that the water and the auxiliary material that add 20-25 times of volume boil, and remove slag then and get juice, again juice is added fermentor tank, water vapor deactivation under the normal temperature and pressure is cooled to 26 ± 2 ℃, in fermentor tank, inject parent edible fungus kind, submerged fermentation at last.
3, the described method of claim 2, the described submerged fermentation time is 60-72 hour, effective solid contents 〉=70g/L when described submerged fermentation stops.
4, claim 1 or 2 described methods, described fermentor tank are that the patent No. is 03270560.3, and name is called the fermentor tank of putting down in writing in the utility model patent of " cover of edible fungi liquid strain culture tank and culture tank "; It is 200320126611.8 that described inactivation technology adopts the patent No., and name is called the inactivating device of putting down in writing in the utility model patent of " saturation steam pressure-less boiler "; Patent No. 200320126960.X is adopted in the injection of described parent edible fungus kind, and name is called the syringe of putting down in writing in the utility model patent of " edible fungus liquid injection rod and comprise the syringe of this injection rod ".
5, the described method of claim 2, described Callicarpa dichotoma (Lour.) K.Koch leach liquor is the leach liquor of Callicarpa dichotoma (Lour.) K.Koch skin, stem and/or root, edible mushrooms is the female kind of black fungus.
6, the described method of claim 2, described Callicarpa dichotoma (Lour.) K.Koch leach liquor are the leach liquor of Callicarpa dichotoma (Lour.) K.Koch flower, and edible mushrooms is the female kind of black fungus.
7, the described method of claim 2, described Callicarpa dichotoma (Lour.) K.Koch leach liquor is the leach liquor of Callicarpa dichotoma (Lour.) K.Koch root, edible mushrooms is the female kind of Cordyceps militaris (L.) Link..
8, the goods of method acquisition according to claim 5.
9, the described goods of claim 8 are treated application in the mastotic medicine of ulcer not in preparation.
10, the goods of method acquisition according to claim 6.
11, the described goods of claim 10 are treated application in the mastotic medicine of ulcer in preparation.
12, the goods of method acquisition according to claim 7.
13, the application of the described goods of claim 12 in the medicine of preparation treatment cerebral concussion and sequela thereof.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100626547A CN100494342C (en) | 2007-01-12 | 2007-01-12 | Blend fermentation method of Callicarpa dichotoma (Lour.) K.Koch. and edible fungus, product therefrom and its uses |
| PCT/CN2008/070069 WO2008086750A1 (en) | 2007-01-12 | 2008-01-10 | Mixed fermentation method of callicarpa dichotoma (lour.) k. koch and edible fungus, prepared products via the method and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100626547A CN100494342C (en) | 2007-01-12 | 2007-01-12 | Blend fermentation method of Callicarpa dichotoma (Lour.) K.Koch. and edible fungus, product therefrom and its uses |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1995323A true CN1995323A (en) | 2007-07-11 |
| CN100494342C CN100494342C (en) | 2009-06-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100626547A Expired - Fee Related CN100494342C (en) | 2007-01-12 | 2007-01-12 | Blend fermentation method of Callicarpa dichotoma (Lour.) K.Koch. and edible fungus, product therefrom and its uses |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN100494342C (en) |
| WO (1) | WO2008086750A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008086750A1 (en) * | 2007-01-12 | 2008-07-24 | Du Peng | Mixed fermentation method of callicarpa dichotoma (lour.) k. koch and edible fungus, prepared products via the method and uses thereof |
| CN102133283A (en) * | 2011-03-18 | 2011-07-27 | 彭都 | Medicine for treating constipation and preparation method thereof |
| CN103371367A (en) * | 2012-04-26 | 2013-10-30 | 彭都 | Food for acquired immune deficiency syndrome nutritional therapy |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646726A (en) * | 2019-10-11 | 2021-04-13 | 孙傲 | Culture medium for black fungus fermentation, fermentation method and fermented beverage |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2659124Y (en) * | 2003-08-18 | 2004-12-01 | 彭都 | Pot lid of edible mushroom liquid growth pot, and culture pot |
| CN2672466Y (en) * | 2003-12-08 | 2005-01-19 | 沈小茂 | Saturated steam pressure less boiler |
| CN2684548Y (en) * | 2003-12-16 | 2005-03-16 | 沈小茂 | Ediable mushroom liquip injection rod and injector including the same rod |
| CN100494342C (en) * | 2007-01-12 | 2009-06-03 | 彭都 | Blend fermentation method of Callicarpa dichotoma (Lour.) K.Koch. and edible fungus, product therefrom and its uses |
-
2007
- 2007-01-12 CN CNB2007100626547A patent/CN100494342C/en not_active Expired - Fee Related
-
2008
- 2008-01-10 WO PCT/CN2008/070069 patent/WO2008086750A1/en active Application Filing
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008086750A1 (en) * | 2007-01-12 | 2008-07-24 | Du Peng | Mixed fermentation method of callicarpa dichotoma (lour.) k. koch and edible fungus, prepared products via the method and uses thereof |
| CN102133283A (en) * | 2011-03-18 | 2011-07-27 | 彭都 | Medicine for treating constipation and preparation method thereof |
| CN102133283B (en) * | 2011-03-18 | 2012-11-14 | 彭都 | Medicine for treating constipation and preparation method thereof |
| CN103371367A (en) * | 2012-04-26 | 2013-10-30 | 彭都 | Food for acquired immune deficiency syndrome nutritional therapy |
| WO2013159730A1 (en) * | 2012-04-26 | 2013-10-31 | Peng Du | Food for nutritional therapy of aids |
| CN103371367B (en) * | 2012-04-26 | 2015-01-07 | 彭都 | Food for acquired immune deficiency syndrome nutritional therapy |
| US9867851B2 (en) | 2012-04-26 | 2018-01-16 | Du Peng | Food for nutritional therapy of AIDS |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100494342C (en) | 2009-06-03 |
| WO2008086750A1 (en) | 2008-07-24 |
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