CN1966499B - Hypercritical fluid extraction method for olive flavone - Google Patents
Hypercritical fluid extraction method for olive flavone Download PDFInfo
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- CN1966499B CN1966499B CN2005100573856A CN200510057385A CN1966499B CN 1966499 B CN1966499 B CN 1966499B CN 2005100573856 A CN2005100573856 A CN 2005100573856A CN 200510057385 A CN200510057385 A CN 200510057385A CN 1966499 B CN1966499 B CN 1966499B
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Abstract
The invention relates to a method for extracting flavonoids from plants. Against the existed extraction methods, there are organic solvents, like methanol, ethyl acetate, acetone, etc., with the drawbacks of large amount, many varieties, influences on environment, and cumbersome steps, therefore a simple method is presented to extract olive flavonoids. The method includes cleaning olives, drying, crushing, mixing with 95% ethanol and liquid carbon dioxide (>= 99.99%) at a weight ratio of 1:99-15:85, flowing in a heat exchanger and then in a extraction kettle under 8-45 MPa at 30 DEG C to 90 DEG C, flowing into a separation kettle under 7-12 MPa after extraction, entering a vacuum device for condensation after separation, and spray-drying to obtain the finally product.
Description
One, technical field
The present invention relates to from plant extraction flavones method, especially hypercritical fluid extraction method for olive flavone.
Two, background technology
Flavonoid compound is the important natural organic-compound of a class at each positions such as the root that extensively is present in plant, heartwood, bark, leaf, fruit, flower.Flavonoid compound in the plant of finding has the activity of vitamins C sample at present, once once is being regarded as vitamin P, and France and Russia will be considered as vitamin P by flavonoid compound so far.The anti-oil peroxidation of flavones acts on and promptly is proved the sixties in last century, and people such as Pratt have studied Antioxidative Property of Flavonoid, thinks that flavones is as the one-level antioxidant in action, has significant oxidation-resistance.Since the eighties in last century, the research of flavonoid compound has been turned on ability of its removing free radical, the anti-ageing prophylactic-therapeutic effect that reaches senile disease gradually.
Adopt at present solvent extraction flavones method, exist used organic solvent such as methyl alcohol, ethyl acetate, acetone etc., its shortcoming is that consumption of organic solvent is big, and kind is many, and environment is had considerable influence, and weak point such as its complex steps.
Three, summary of the invention
It is simple to the purpose of this invention is to provide a kind of method, and extracting power is strong, supercritical CO
2The olive flavones extracting method that extraction temperature is low.
Technical scheme that the present invention adopts is a supercritical CO
2Extraction, its method steps is as follows:
(1) raw material is prepared: olive is through cleaning, dry, pulverize the input extraction kettle;
(2) supercritical CO
2Extraction: with 95% ethanol and 〉=99.99% liquid CO
2By 1: 99-15: 85 weight ratios reach supercritical state through heat exchanger after mixing in mixing tank, feed extraction kettle again, and the still internal pressure is that 8-45MPa, temperature are 30 ℃-90 ℃;
(3) separate: extraction liquid flows in the separating still in the extraction kettle, the separating still internal pressure is 7-12MPa, temperature is 30 ℃-90 ℃, collect extraction liquid from separating still with collector again, again the extraction liquid in the collector is injected vacuum concentrating apparatus, be concentrated to the 1-2 of raw material weight ratio doubly, after entering spray-drier at last and carrying out spray drying treatment, get product.
Supercritical liquid extraction technique of the present invention is to utilize the fluid that is in more than emergent pressure and the critical temperature to have the high-solvency and the high penetrating power of special increase, and some component in the material is carried out extracting and separating.And the supercutical fluid that uses is a carbonic acid gas, nontoxic because of it, do not burn, to most of material do not react, characteristics such as inexpensive, under supercritical state, CO
2Fluid has the dual characteristics of gas-liquid two-phase concurrently, has both had high spread coefficient and the low viscosity suitable with gas, has density close with liquid and material good dissolving ability again.
It is simple that the present invention has method, and extracting power is strong, extraction yield height, supercritical CO
2Characteristics such as extraction temperature is low, can preserve effective ingredients in plant more in good condition and not be destroyed, and extraction time is fast, and is with short production cycle.
Four, embodiment
Lift one below and implement former example, further specify invention.
The 10 kilogram emblica powders of olive through cleaning, dry, be ground into the 1mm granularity are dropped into 25 liters of extraction kettles, with 95% ethanol and 99.99% liquid CO
2By after 5: 95 weight ratios mix in mixing tank, reach supercritical state through heat exchanger, feed 25 liters extraction kettle again, the still internal pressure is that 25MPa, temperature are 50 ℃, 25 liters of interior extraction liquids of extraction kettle flow in 10 liters of separating stills, 10 liters of separating still internal pressures are that 9MPa, temperature are 55 ℃, collect collector by the effusive extraction liquid of separating still, again the extraction liquid in the collector is injected vacuum concentrating apparatus, be concentrated to about 12 kilograms, enter spray-drier at last and carry out spray drying treatment, get finished product 644 grams, its general flavone content is 42.87%.
Claims (1)
1. hypercritical fluid extraction method for olive flavone is characterized in that method steps is as follows:
(1) raw material is prepared: olive is through cleaning, dry, pulverize the input extraction kettle;
(2) supercritical CO
2Extraction: with 95% ethanol and 〉=99.99% liquid CO
2By 1: 99-15: 85 weight ratios reach supercritical state through heat exchanger after mixing in mixing tank, feed extraction kettle again, and the still internal pressure is that 8-45MPa, temperature are 30 ℃-90 ℃;
(3) separate: extraction liquid flows into separating still in the extraction kettle, the separating still internal pressure is 7-12MPa, temperature is 30 ℃-90 ℃, collect extraction liquid from separating still with collector again, again extraction liquid in the collector is injected vacuum concentrating apparatus, be concentrated to the 1-2 of raw material weight ratio doubly, after entering spray-drier at last and carrying out spray drying treatment, get product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100573856A CN1966499B (en) | 2005-11-18 | 2005-11-18 | Hypercritical fluid extraction method for olive flavone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100573856A CN1966499B (en) | 2005-11-18 | 2005-11-18 | Hypercritical fluid extraction method for olive flavone |
Publications (2)
| Publication Number | Publication Date |
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| CN1966499A CN1966499A (en) | 2007-05-23 |
| CN1966499B true CN1966499B (en) | 2011-04-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2005100573856A Active CN1966499B (en) | 2005-11-18 | 2005-11-18 | Hypercritical fluid extraction method for olive flavone |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110835569A (en) * | 2019-11-21 | 2020-02-25 | 山西焦煤集团有限责任公司 | Method for removing organic sulfur in coal by using supercritical fluid hydrogenation reaction |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444557B (en) * | 2008-12-30 | 2011-08-10 | 新疆医科大学 | Method for producing general flavone of medicinal mulberry by supercritical carbon dioxide extraction |
| CN101805664B (en) * | 2010-03-29 | 2013-07-03 | 甘肃省轻工研究院 | Method and device for extracting olive pomace oil from olive pomace |
| CN106668116A (en) * | 2016-12-27 | 2017-05-17 | 云南中烟工业有限责任公司 | Supercritical extraction method for improving oleuropein flavor quality |
| CN112774245A (en) * | 2019-11-07 | 2021-05-11 | 福建仙芝楼生物科技有限公司 | A method for extracting and separating active extract of semen Canarii albi by supercritical technology |
| CN114774490A (en) * | 2022-04-19 | 2022-07-22 | 沈阳市丽晨生物医药科技有限公司 | Preparation method of dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases |
-
2005
- 2005-11-18 CN CN2005100573856A patent/CN1966499B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| F. Le Floch et al..Supercritical fluid extraction of phenol compounds from olive leaves.《Talanta》.1998,第46卷第1123-1130页. * |
| 唐春红.野生橄榄总黄酮提取方法研究.《渝州大学学报(自然科学版)》.2000,第17卷(第4期),第70-73页. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110835569A (en) * | 2019-11-21 | 2020-02-25 | 山西焦煤集团有限责任公司 | Method for removing organic sulfur in coal by using supercritical fluid hydrogenation reaction |
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| CN1966499A (en) | 2007-05-23 |
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