CN114774490A - Preparation method of dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases - Google Patents
Preparation method of dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases Download PDFInfo
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- CXQWRCVTCMQVQX-LSDHHAIUSA-N (+)-taxifolin Chemical compound C1([C@@H]2[C@H](C(C3=C(O)C=C(O)C=C3O2)=O)O)=CC=C(O)C(O)=C1 CXQWRCVTCMQVQX-LSDHHAIUSA-N 0.000 title claims abstract description 75
- XCGZWJIXHMSSQC-UHFFFAOYSA-N dihydroquercetin Natural products OC1=CC2OC(=C(O)C(=O)C2C(O)=C1)c1ccc(O)c(O)c1 XCGZWJIXHMSSQC-UHFFFAOYSA-N 0.000 title claims abstract description 75
- 208000024172 Cardiovascular disease Diseases 0.000 title claims abstract description 11
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 10
- 201000011510 cancer Diseases 0.000 title claims abstract description 10
- 208000026106 cerebrovascular disease Diseases 0.000 title claims abstract description 10
- 230000003647 oxidation Effects 0.000 title claims abstract description 10
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 10
- 230000002526 effect on cardiovascular system Effects 0.000 title claims abstract description 9
- 238000002360 preparation method Methods 0.000 title description 3
- 239000000284 extract Substances 0.000 claims abstract description 45
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 241000218652 Larix Species 0.000 claims abstract description 38
- 235000005590 Larix decidua Nutrition 0.000 claims abstract description 38
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000002156 mixing Methods 0.000 claims abstract description 29
- 238000000605 extraction Methods 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 19
- 108010059892 Cellulase Proteins 0.000 claims abstract description 16
- 229940106157 cellulase Drugs 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 16
- 239000011259 mixed solution Substances 0.000 claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 229940088598 enzyme Drugs 0.000 claims abstract description 14
- 239000000243 solution Substances 0.000 claims abstract description 13
- 238000004566 IR spectroscopy Methods 0.000 claims abstract description 10
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims abstract description 7
- 238000012216 screening Methods 0.000 claims abstract description 7
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 5
- 230000031700 light absorption Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 238000002329 infrared spectrum Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 238000002835 absorbance Methods 0.000 description 5
- 239000003963 antioxidant agent Substances 0.000 description 4
- 230000003078 antioxidant effect Effects 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 102000042822 P family Human genes 0.000 description 1
- 108091082789 P family Proteins 0.000 description 1
- 239000008681 Phellinus linteus extract Substances 0.000 description 1
- ZONYXWQDUYMKFB-UHFFFAOYSA-N SJ000286395 Natural products O1C2=CC=CC=C2C(=O)CC1C1=CC=CC=C1 ZONYXWQDUYMKFB-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229930003949 flavanone Natural products 0.000 description 1
- -1 flavanone compounds Chemical class 0.000 description 1
- 235000011981 flavanones Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- IKGXIBQEEMLURG-NVPNHPEKSA-N rutin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-NVPNHPEKSA-N 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/32—2,3-Dihydro derivatives, e.g. flavanones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
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Abstract
The invention discloses a method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases, which comprises the steps of mixing enzyme with cellulase and larch powder with a predetermined granularity for enzymolysis to obtain larch enzymolysis liquid; mixing the larch enzymatic hydrolysate with an 81% ethanol solution according to the proportion of 1-5:3-10, and then placing the obtained mixed solution into an ultrasonic extraction instrument for extraction for 62-100 minutes to obtain an extract liquor; then putting the extract into a high-pressure kettle, pressurizing and heating the extract, and crystallizing the extract to obtain dihydroquercetin; and finally, measuring the crystallized dihydroquercetin by infrared spectroscopy, and screening the dihydroquercetin with the purity of more than 90% to obtain a refined dihydroquercetin product. Therefore, the purity of the dihydroquercetin extracted under different conditions can be accurately determined, the extraction rate is improved, and the purity can be accurately determined.
Description
Technical Field
The invention relates to the technical field of dihydroquercetin, and in particular relates to a method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases.
Background
Dihydroquercetin is a natural medicine with remarkable antioxidation, can be used as main and auxiliary raw materials of medicines and bioactive additives of foods, and can be widely applied to medicines and health-care functional foods. Has wide application in the fields of medicine, industry and agriculture. Dihydroquercetin belongs to flavanone compounds, vitamin P family, is a strong antioxidant, can adsorb free radicals in natural antioxidant up to 32 mu mol/mg (purity of dihydroquercetin is 95%), and has obvious effect on prolonging cell life by scavenging free radicals as a natural antioxidant, so that the dihydroquercetin has strong biological activity and medicinal value. In the related art, the purity of dihydroquercetin extracted under different conditions at present has certain difference, so that the extraction rate is low and the purity is difficult to determine accurately.
Disclosure of Invention
The present invention is directed to solving, at least in part, one of the technical problems in the related art. Therefore, one objective of the present invention is to provide a method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases, which comprises the following steps:
s10, mixing enzyme with cellulase and larch powder with a preset granularity for enzymolysis to obtain larch enzymolysis liquid;
s20, mixing the larch enzymolysis liquid with an 81% ethanol solution according to the proportion of 1-5:3-10, and placing the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 62-100 minutes to obtain an extraction liquid;
s30, putting the extraction liquid into a high-pressure kettle, pressurizing and heating, and crystallizing the extraction liquid to obtain dihydroquercetin;
s40, measuring the crystallized dihydroquercetin by infrared spectroscopy, and screening the dihydroquercetin with the purity of more than 90% to obtain a refined dihydroquercetin product.
Optionally, the step S10 includes:
providing larch powder and deionized water, and mixing according to a ratio of 1:15 to obtain a mixed solution;
mixing the enzyme with the cellulase and the mixed solution for enzymolysis for 2-2.5 hours, and adding the mixture into a centrifuge to centrifuge for 5-10 minutes at the rotating speed of 6000 r/min;
after the centrifugation is completed, the supernatant is collected to obtain a larch enzymolysis solution.
Optionally, the predetermined particle size is 0.11mm to 0.16 mm.
Optionally, the frequency of the ultrasonic extractor is 5MHZ, the pulse period frequency is 5 times/second, and the acoustic beam is 1000 m/s.
Optionally, the step S30 includes:
putting the extraction liquid into an autoclave, and pressurizing to 550-580 Mpa;
reducing the pressure after the extract liquor is kept in the pressure state for 12 minutes, and heating the extract liquor to 92 ℃ while keeping continuous stirring;
and standing the extract for 4 days at the temperature of 23-28 ℃ after the extract is cooled to obtain the crystallized dihydroquercetin.
Optionally, the step S40 includes:
selecting 5 samples of crystallized dihydroquercetin, and selecting 2-7g of dihydroquercetin in each sample to be dissolved in 99.5% ethanol;
measuring each sample by adopting an infrared spectrum with a preset wavelength, and determining the light absorption intensity of each sample;
and screening out the dihydroquercetin refined product with the purity of more than 90 percent according to the light absorption intensity of each sample.
Optionally, the predetermined wavelength is 235nm-270 nm.
Optionally, the cellulase is used in an amount of 2.5-2.9% of the total mass of the larch powder; the enzyme activity of the cellulase is more than or equal to 6 wu/g.
The invention provides a method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases, which comprises the steps of mixing enzyme with cellulase and larch powder with a predetermined granularity for enzymolysis to obtain larch enzymolysis liquid; mixing the larch enzymolysis liquid and an ethanol solution with the concentration of 81% according to the proportion of 1-5:3-10, and then placing the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 62-100 minutes to obtain an extract liquid; then putting the extract into a high-pressure kettle, pressurizing and heating the extract, and crystallizing the extract to obtain dihydroquercetin; and finally, measuring the crystallized dihydroquercetin by infrared spectroscopy, and screening the dihydroquercetin with the purity of more than 90% to obtain a dihydroquercetin refined product. Therefore, the purity of the dihydroquercetin extracted under different conditions can be accurately determined, the extraction rate is improved, and the purity can be accurately determined.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The following detailed description of the embodiments of the present invention is intended to illustrate the present invention and should not be construed as limiting the present invention, and all other embodiments that can be obtained by one skilled in the art without inventive faculty based on the embodiments of the present invention shall fall within the scope of the present invention.
The method for preparing dihydroquercetin for anticancer, antioxidant and anti-cardiovascular and cerebrovascular diseases according to the embodiment of the invention is described in detail below.
According to the method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases, the dihydroquercetin is prepared under different conditions in the following first to fifth embodiments, and the purity of the dihydroquercetin is measured under different conditions.
First embodiment
The preparation method of the phellinus linteus extract provided in the first embodiment of the present invention comprises:
providing 0.12nm larch powder and deionized water, and mixing according to a ratio of 1:15 to obtain a mixed solution;
mixing enzyme with cellulase and the mixed solution for enzymolysis for 2 hours, and adding into a centrifuge to centrifuge for 6 minutes at a rotating speed of 6000 r/min;
after the centrifugation is finished, collecting the supernatant to obtain larch enzymolysis liquid;
mixing the larch enzymolysis liquid and an ethanol solution with the concentration of 81% according to the proportion of 1:4, and then putting the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 67 minutes to obtain an extract liquid;
putting the extract into a high-pressure kettle, and pressurizing to 550 Mpa;
after the extract liquor is kept in a pressure state for 12 minutes, reducing the pressure, and raising the temperature of the extract liquor to 92 ℃ while keeping continuously stirring;
standing the extract for 4 days at 24 deg.C after cooling to obtain crystallized dihydroquercetin;
taking the crystallized dihydroquercetin as a sample, and selecting 3g to be dissolved in 99.5% ethanol;
the sample was measured by infrared spectroscopy at 240nm to determine the corresponding absorbance intensity.
Second embodiment
The method for extracting dihydroquercetin provided in the second embodiment of the invention comprises the following steps:
providing 0.14nm larch powder and deionized water, and mixing according to a ratio of 1:15 to obtain a mixed solution;
mixing enzyme with cellulase and the mixed solution for enzymolysis for 2 hours, and adding into a centrifuge to centrifuge for 8 minutes at a rotating speed of 6000 r/min;
after the centrifugation is finished, collecting the supernatant to obtain larch enzymolysis liquid;
mixing the larch enzymolysis liquid and an ethanol solution with the concentration of 81% according to the proportion of 2:9, and then placing the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 80 minutes to obtain an extract liquid;
putting the extract into a high-pressure kettle, and pressurizing to 570 Mpa;
after the extract liquor is kept in a pressure state for 12 minutes, reducing the pressure, and raising the temperature of the extract liquor to 92 ℃ while keeping continuously stirring;
standing the extract for 4 days at 27 deg.C after cooling to obtain crystallized dihydroquercetin;
taking the crystallized dihydroquercetin as a sample, and selecting 3g of dihydroquercetin to be dissolved in 99.5% ethanol;
the sample was measured by infrared spectroscopy at 240nm to determine the corresponding absorbance intensity.
Third embodiment
The method for extracting dihydroquercetin provided in the third embodiment of the invention comprises the following steps:
providing 0.11nm larch powder and deionized water, and mixing according to a ratio of 1:15 to obtain a mixed solution;
mixing enzyme with cellulase and the mixed solution for enzymolysis for 2 hours, and adding into a centrifuge to centrifuge for 8 minutes at a rotating speed of 6000 r/min;
after the centrifugation is finished, collecting the supernatant to obtain larch enzymolysis liquid;
mixing the larch enzymolysis liquid and an ethanol solution with the concentration of 81% according to the proportion of 3:10, and then putting the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 75 minutes to obtain an extract liquid;
putting the extract into a high-pressure kettle, and pressurizing to 570 Mpa;
after the extract liquor is kept in a pressure state for 12 minutes, reducing the pressure, and raising the temperature of the extract liquor to 92 ℃ while keeping continuously stirring;
standing the extract for 4 days at 27 deg.C after cooling to obtain crystallized dihydroquercetin;
taking the crystallized dihydroquercetin as a sample, and selecting 6g of dihydroquercetin to be dissolved in 99.5% ethanol;
the sample was measured by infrared spectroscopy at 240nm to determine the corresponding absorbance intensity.
Fourth embodiment
The method for extracting dihydroquercetin provided in the fourth embodiment of the invention comprises the following steps:
providing 0.15nm particle size larch powder and deionized water, and mixing according to a ratio of 1:15 to obtain a mixed solution;
mixing enzyme with cellulase and the mixed solution for enzymolysis for 2.5 hours, and adding into a centrifuge to centrifuge for 8 minutes at a rotating speed of 6000 r/min;
after the centrifugation is finished, collecting the supernatant to obtain larch enzymolysis liquid;
mixing the larch enzymolysis liquid and an ethanol solution with the concentration of 81% according to the ratio of 2:7, and then putting the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 90 minutes to obtain an extract liquid;
putting the extract into a high-pressure kettle, and pressurizing to 580 Mpa;
reducing the pressure after the extract liquor is kept in a pressure state for 12 minutes, and heating the extract liquor to 92 ℃ while keeping continuous stirring;
standing the extract for 4 days at 25 deg.C after the extract is cooled to obtain crystallized dihydroquercetin;
taking the crystallized dihydroquercetin as a sample, and selecting 6g of dihydroquercetin to be dissolved in 99.5% ethanol;
the sample was measured by infrared spectroscopy at 240nm to determine the corresponding absorbance intensity.
Fifth embodiment
The method for extracting dihydroquercetin provided in the fifth embodiment of the present invention includes:
providing 0.11nm particle size larch powder and deionized water, and mixing according to a ratio of 1:15 to obtain a mixed solution;
mixing enzyme with cellulase and the mixed solution for enzymolysis for 2.5 hours, and adding into a centrifuge to centrifuge for 10 minutes at a rotating speed of 6000 r/min;
after the centrifugation is finished, collecting the supernatant to obtain larch enzymolysis liquid;
mixing the larch enzymolysis liquid and an ethanol solution with the concentration of 81% according to the proportion of 1:9, and then putting the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 90 minutes to obtain an extract liquid;
putting the extract into a high-pressure kettle, and pressurizing to 580 Mpa;
reducing the pressure after the extract liquor is kept in a pressure state for 12 minutes, and heating the extract liquor to 92 ℃ while keeping continuous stirring;
standing the extract for 4 days at 25 deg.C after the extract is cooled to obtain crystallized dihydroquercetin;
taking the crystallized dihydroquercetin as a sample, and selecting 6g of dihydroquercetin to be dissolved in 99.5% ethanol;
the sample was measured by infrared spectroscopy at 240nm to determine the corresponding absorbance intensity.
The following table and the first to fifth embodiments are combined, and the test results are shown in the following table.
As can be seen from the comparison of the first to fifth embodiments, in the fifth embodiment, when the particle size of the larch powder is smaller and the pressure is higher, the corresponding absorption intensity is higher, i.e. the corresponding purity is higher, so that the extraction of dihydroquercetin can be performed under the conditions of the fifth embodiment, thereby increasing the extraction rate.
The invention provides a method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases, which comprises the steps of mixing enzyme with cellulase and larch powder with a predetermined granularity for enzymolysis to obtain larch enzymolysis liquid; mixing the larch enzymolysis liquid and an ethanol solution with the concentration of 81% according to the proportion of 1-5:3-10, and then putting the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 62-100 minutes to obtain an extract liquid; then putting the extract into a high-pressure kettle, pressurizing and heating the extract, and crystallizing the extract to obtain dihydroquercetin; and finally, measuring the crystallized dihydroquercetin by infrared spectroscopy, and screening the dihydroquercetin with the purity of more than 90% to obtain a refined dihydroquercetin product. Therefore, the purity of the dihydroquercetin extracted under different conditions can be accurately determined, the extraction rate is improved, and the purity can be accurately determined.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Moreover, various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without being mutually inconsistent.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all applications directly/indirectly applied to other related fields under the inventive concept of the present invention are included in the scope of the present invention.
Claims (8)
1. A method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases is characterized by comprising the following steps:
s10, mixing enzyme with cellulase and larch powder with a preset granularity for enzymolysis to obtain larch enzymolysis liquid;
s20, mixing the larch enzymolysis liquid with an 81% ethanol solution according to the proportion of 1-5:3-10, and placing the obtained mixed liquid into an ultrasonic extraction instrument for extraction for 62-100 minutes to obtain an extraction liquid;
s30, putting the extraction liquid into a high-pressure kettle, pressurizing and heating, and crystallizing the extraction liquid to obtain dihydroquercetin;
s40, measuring the crystallized dihydroquercetin by infrared spectroscopy, and screening the dihydroquercetin with the purity of more than 90% to obtain a refined dihydroquercetin product.
2. The method for preparing dihydroquercetin according to claim 1, wherein the step S10 includes:
providing larch powder and deionized water, and mixing the larch powder and the deionized water according to the proportion of 1:15 to obtain a mixed solution;
mixing the enzyme with the cellulase and the mixed solution for enzymolysis for 2-2.5 hours, and adding the mixture into a centrifuge to centrifuge for 5-10 minutes at the rotating speed of 6000 r/min;
after the centrifugation is completed, the supernatant is collected to obtain a larch enzymolysis solution.
3. The method for preparing dihydroquercetin for resisting cancer, oxidation and cardiovascular diseases according to claim 1, wherein the predetermined particle size is 0.11mm-0.16 mm.
4. The method for preparing dihydroquercetin according to claim 1, wherein the frequency of the ultrasonic extractor is 5MHZ, the pulse period frequency is 5 times/s, and the sound beam is 1000 m/s.
5. The method for preparing dihydroquercetin according to claim 1, wherein the step S30 includes:
putting the extraction liquid into an autoclave, and pressurizing to 550-580 MPa;
reducing the pressure after the extract liquor is kept in the pressure state for 12 minutes, and heating the extract liquor to 92 ℃ while keeping continuous stirring;
standing for 4 days at 23-28 deg.C after the extractive solution is cooled to obtain crystallized dihydroquercetin.
6. The method for preparing dihydroquercetin according to claim 1, wherein the step S40 includes:
selecting 5 samples of crystallized dihydroquercetin, and selecting 2-7g of dihydroquercetin in each sample to be dissolved in 99.5% ethanol;
measuring each sample by adopting an infrared spectrum with a preset wavelength, and determining the light absorption intensity of each sample;
and screening out the refined dihydroquercetin product with the purity of more than 90% according to the light absorption intensity of each sample.
7. The method for preparing dihydroquercetin according to claim 6, wherein the predetermined wavelength is 235nm-270 nm.
8. The method for preparing dihydroquercetin according to claim 1, wherein the dosage of the cellulase is 2.5-2.9% of the total weight of the larch powder; the enzyme activity of the cellulase is more than or equal to 6 ten thousand u/g.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1966499A (en) * | 2005-11-18 | 2007-05-23 | 重庆锦程实业有限公司 | Hypercritical fluid extraction method for olive flavone |
| CN101830881A (en) * | 2010-04-28 | 2010-09-15 | 东北林业大学 | Enzyme-induced method for efficiently extracting toxifolin from larch processing leftover |
| CN103054907A (en) * | 2012-12-05 | 2013-04-24 | 天津隆顺榕发展制药有限公司 | Propolis flavonoid extractive and preparation method thereof |
| CN104710393A (en) * | 2015-04-09 | 2015-06-17 | 蔡兴福 | Pretreatment ethanol extraction method of dihydroquercetin in larix gmelini wood powder |
| CN107216304A (en) * | 2017-07-24 | 2017-09-29 | 江苏大学 | A kind of method of dihydroquercetin in ultrasound assisted extraction sorghum bran |
| CN108285440A (en) * | 2017-05-20 | 2018-07-17 | 郑毅男 | A kind of preparation method for extracting dihydroquercetin extract in larch using ultra-high pressure apparatus |
-
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1966499A (en) * | 2005-11-18 | 2007-05-23 | 重庆锦程实业有限公司 | Hypercritical fluid extraction method for olive flavone |
| CN101830881A (en) * | 2010-04-28 | 2010-09-15 | 东北林业大学 | Enzyme-induced method for efficiently extracting toxifolin from larch processing leftover |
| CN103054907A (en) * | 2012-12-05 | 2013-04-24 | 天津隆顺榕发展制药有限公司 | Propolis flavonoid extractive and preparation method thereof |
| CN104710393A (en) * | 2015-04-09 | 2015-06-17 | 蔡兴福 | Pretreatment ethanol extraction method of dihydroquercetin in larix gmelini wood powder |
| CN108285440A (en) * | 2017-05-20 | 2018-07-17 | 郑毅男 | A kind of preparation method for extracting dihydroquercetin extract in larch using ultra-high pressure apparatus |
| CN107216304A (en) * | 2017-07-24 | 2017-09-29 | 江苏大学 | A kind of method of dihydroquercetin in ultrasound assisted extraction sorghum bran |
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