CN1961897B - 一种石榴皮提取物的鉴别方法 - Google Patents
一种石榴皮提取物的鉴别方法 Download PDFInfo
- Publication number
- CN1961897B CN1961897B CN2005100220426A CN200510022042A CN1961897B CN 1961897 B CN1961897 B CN 1961897B CN 2005100220426 A CN2005100220426 A CN 2005100220426A CN 200510022042 A CN200510022042 A CN 200510022042A CN 1961897 B CN1961897 B CN 1961897B
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- CN
- China
- Prior art keywords
- extract
- galla chinensis
- pericarpium granati
- aqueous solution
- phosphate aqueous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 235000014360 Punica granatum Nutrition 0.000 title claims abstract description 7
- 241000219991 Lythraceae Species 0.000 title claims abstract description 5
- 239000000284 extract Substances 0.000 title claims description 42
- 241000283690 Bos taurus Species 0.000 title 1
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 69
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 36
- 229910019142 PO4 Inorganic materials 0.000 claims description 30
- 239000007864 aqueous solution Substances 0.000 claims description 30
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 30
- 239000010452 phosphate Substances 0.000 claims description 30
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 claims description 27
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 claims description 27
- 229920002079 Ellagic acid Polymers 0.000 claims description 27
- 229960002852 ellagic acid Drugs 0.000 claims description 27
- 235000004132 ellagic acid Nutrition 0.000 claims description 27
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 claims description 27
- 239000013558 reference substance Substances 0.000 claims description 15
- 238000012850 discrimination method Methods 0.000 claims description 13
- 239000000523 sample Substances 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000000287 crude extract Substances 0.000 claims description 4
- 239000000945 filler Substances 0.000 claims description 4
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 238000005374 membrane filtration Methods 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims 1
- 239000012488 sample solution Substances 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000003908 quality control method Methods 0.000 abstract description 2
- 239000012071 phase Substances 0.000 description 24
- 238000002474 experimental method Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 244000294611 Punica granatum Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 102000005862 Angiotensin II Human genes 0.000 description 1
- 101800000733 Angiotensin-2 Proteins 0.000 description 1
- CZGUSIXMZVURDU-JZXHSEFVSA-N Ile(5)-angiotensin II Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C=CC=CC=1)C([O-])=O)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=[NH2+])NC(=O)[C@@H]([NH3+])CC([O-])=O)C(C)C)C1=CC=C(O)C=C1 CZGUSIXMZVURDU-JZXHSEFVSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 229950006323 angiotensin ii Drugs 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- 150000008442 polyphenolic compounds Chemical group 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000013062 quality control Sample Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
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- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
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- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2005100220426A CN1961897B (zh) | 2005-11-09 | 2005-11-09 | 一种石榴皮提取物的鉴别方法 |
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CN2005100220426A CN1961897B (zh) | 2005-11-09 | 2005-11-09 | 一种石榴皮提取物的鉴别方法 |
Publications (2)
Publication Number | Publication Date |
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CN1961897A CN1961897A (zh) | 2007-05-16 |
CN1961897B true CN1961897B (zh) | 2012-05-23 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN2005100220426A Expired - Fee Related CN1961897B (zh) | 2005-11-09 | 2005-11-09 | 一种石榴皮提取物的鉴别方法 |
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CN (1) | CN1961897B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106124684B (zh) * | 2016-06-02 | 2018-03-06 | 浙江金大康动物保健品有限公司 | 一种石榴皮止泻散的定性与定量检测方法 |
CN107247095B (zh) * | 2017-05-25 | 2019-08-20 | 武汉大学 | 一种基于代谢组学分析的用于抗氧化衰老药物筛选的大鼠模型的构建方法 |
CN108956800B (zh) * | 2018-05-18 | 2021-07-23 | 山东省食品药品检验研究院 | 牡丹皮中鞣花酸含量的测定方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002205993A (ja) * | 2001-01-10 | 2002-07-23 | Fuji Chemical Ind Co Ltd | エラグ酸の製造方法 |
US6428826B1 (en) * | 1999-10-07 | 2002-08-06 | Bioactiva Microtechne | Chilco product and method of making and using same |
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2005
- 2005-11-09 CN CN2005100220426A patent/CN1961897B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6428826B1 (en) * | 1999-10-07 | 2002-08-06 | Bioactiva Microtechne | Chilco product and method of making and using same |
JP2002205993A (ja) * | 2001-01-10 | 2002-07-23 | Fuji Chemical Ind Co Ltd | エラグ酸の製造方法 |
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CN1961897A (zh) | 2007-05-16 |
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Owner name: CHENGDU WAGOTT NATURAL PRODUCTS CO., LTD. Free format text: FORMER OWNER: HUAGAO PHARMACEUTICAL CO., LTD., CHENGDU Effective date: 20130107 |
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Effective date of registration: 20130107 Address after: 610041, Sichuan Chengdu Shuangliu County Economic Development Zone, Southwest Airlines Industrial Development Zone Four Patentee after: Chengdu?Wagott?Pharmaceuticals?Co.,?Ltd. Address before: 610041, Sichuan, Chengdu province hi tech Zone Tianfu Road, South extension, Tianhe incubator Park, block A, building 5 Patentee before: Huagao Pharmaceutical Co., Ltd., Chengdu |
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Application publication date: 20070516 Assignee: Huagao Pharmaceutical Co., Ltd., Chengdu Assignor: Chengdu?Wagott?Pharmaceuticals?Co.,?Ltd. Contract record no.: 2014510000120 Denomination of invention: Method for verifying pomegranate rind extract Granted publication date: 20120523 License type: Exclusive License Record date: 20140815 |
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Assignee: Huagao Pharmaceutical Co., Ltd., Chengdu Assignor: Chengdu?Wagott?Pharmaceuticals?Co.,?Ltd. Contract record no.: 2014510000120 Date of cancellation: 20141024 |
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