CN1939468B - Compound Chinese Gall antiseptic prescription for fishing - Google Patents
Compound Chinese Gall antiseptic prescription for fishing Download PDFInfo
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- CN1939468B CN1939468B CN2006100951657A CN200610095165A CN1939468B CN 1939468 B CN1939468 B CN 1939468B CN 2006100951657 A CN2006100951657 A CN 2006100951657A CN 200610095165 A CN200610095165 A CN 200610095165A CN 1939468 B CN1939468 B CN 1939468B
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- chinese gall
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Abstract
An antibacterial and broad-spectrum Chinese medicine for preventing and treating the bacterial diseases of aquatic animals is proportionally prepared from Chinese gall, green chiretta, glavescent sophora root and phellodendron bark.
Description
Technical field
The present invention relates to the veterinary drug technical field, particularly relate to a kind of compound Chinese Gall antiseptic prescription for fishing
Background technology
The main means of at present the aquatic animal bacterial disease being controlled are antibacterials, but use chemical antibacterials, antibiotic medicine ubiquity easily to cause the pathogen drug resistance, cause problems such as the cultured fishes drug residue exceeds standard.Therefore all seeking succedaneum more safely and effectively both at home and abroad.Natural resources of Chinese medicinal materials is very abundant, also has less residually, and side effect is little, meets universally recognized characteristics such as nuisanceless aquaculture requirement, has become the main material that the human research prevents and treats the fish disease medicine.In the relevant both at home and abroad report of Chinese herb prevention fish diseases, have more than 10 piece of document be cmm of simple prescription such as Galla Chinensis respectively to the extracorporeal bacteria inhibitor test of several frequently seen fish bacterial pathogens, the result all demonstrates Galla Chinensis stronger fungistatic effect.It is that the Galla Chinensis folk prescription uses the clinical trial to fish shrimp crab that nearly 10 pieces of documents are arranged, and the result shows that prevention effect is obvious.Zhao Chunguang etc. (2006) select for use 12 flavor Chinese herbal medicine such as Galla Chinensis to be made into 4 prescriptions to carry out the test of Trionyx sinensis Wiegmann ichthyophthiriasis prevention effect, and Galla Chinensis, Fructus Mume, Radix Scutellariae, Herba polygoni hydropiperis, white book box (3: 2: 2: 2: 1) prescription prevention effect are remarkable as a result, cure rate 95%; Each compatibility of Lee state equality (1998) for oral administration treat European eel enteritis disease with 4 prescriptions of external, wherein form exterior-applied prescription by Galla Chinensis and Radix Et Rhizoma Rhei.The narrow antimicrobial spectrum but these are filled a prescription can only be prevented and treated one or more bacteriosis of aquatic livestock, can not prevent and treat the multiple aquatic animal bacterial disease very effectively.Up to the present, the relevant research report of Shang Weijian with compound Chinese Gall fishing antibacterial agent of broad-spectrum antibacterial action.
Summary of the invention
The objective of the invention is to, overcome the problems such as narrow antimicrobial spectrum that existing fishing antibacterial agent exists, a kind of pure Chinese medicinal preparation with broad-spectrum antibacterial action---compound Chinese Gall antiseptic prescription for fishing is provided, control the multiple disease that the aquatic animal common pathogen causes effectively, reduce harm and economic loss that bacteriosis of aquatic livestock causes to fish production thus.
The inventor selects a large amount of single medicinal materials to carry out the bacteriostatic test of fish bacterial disease, therefrom obtain the single medicinal material of good antimicrobial effect, carry out prescription with its variety classes, quantity, ratio, adopt the fungistatic effect test from multiple compound recipe, to filter out the best compound recipe of fungistatic effect again.Its component and content are:
20 parts of Galla Chinensiss, 20 parts of Herba Andrographis, 15 parts of Radix Sophorae Flavescentiss, 15 parts of Cortex Phellodendris
The preparation method of compound Chinese Gall fishing antibacterial agent of the present invention is as follows:
Antibacterial powder method for making: with each component single medicinal material drying, pulverize the back and cross 100 mesh sieves, press formula proportion and mix, promptly make powder.
Antibacterial water preparation method for making: adopt ethanol heating continuous backflow extraction method, get compound Chinese Gall antibacterial powder, in W: V=1: it is 70% ethanol that 8 ratio adds concentration, with hydrochloric acid regulate pH value be 2,76 ℃ down with Soxhlet extraction apparatus reflux, extract, 90 minutes, collect liquid, residue repeats to extract twice again, merges extract, and evaporating into does not have the alcohol flavor, add water to 100ml by per 2 gram powder extracts, promptly make water preparation.
The using method of compound Chinese Gall fishing antibacterial agent is:
Be added in the feedstuff by 0.5%～1.0% weight ratio when the antibacterial powder uses, promptly 1kg antibacterial powder can be added in 200～100kg feedstuff.Be added in the feedstuff in being converted into powder 0.5%～1.0% ratio when the antibacterial water preparation uses, promptly the ratio that is blended in 400～200g feedstuff in the 100ml antibacterial is added; Oral and injected dose is 0.5～2.0ml/kgd, is 0.5～2.0ml by the antibacterial dosage that every day, per kilogram fish body weight gave promptly.
Advantage of the present invention is:
(1) the compound Chinese Gall fishing antibacterial agent has broad-spectrum antibacterial action, can prevent and treat bacteriosis of aquatic livestock effectively.Antibacterial is obvious to the external fungistatic effect of harm aquatic animal common pathogens such as Aeromonas sobria, Aeromonas hydrophila, aeromonas salmonicida, Flexibacter columnaris, tarda, Shandong Ke Shi yersinia, and inhibition zone diameter can reach between 2.0～4.0.The antibacterial test of pesticide effectiveness shows, the bacteriosis of aquatic livestock control rate that this medicament causes above-mentioned common pathogens such as Aeromonas sobrias is between 80%～95%, and the general control rate can reach more than 85%.
(2) compound Chinese Gall fishing antibacterial agent safety non-toxic
Safety is measured: adopt acute toxicity test to carry out the antibacterial safety and measure the grade scale LD of acute toxicity
50＞10mg/kg is nontoxic.Even test shows that the antibacterial dosage has reached 10000mg (crude drug)/kg, poisoning does not still appear in the fish body, total visible antibacterial effective ingredient belongs to actual nontoxic level to the fish body.Drawing Galla Chinensis (single medicinal material) according to " improvement karber's method " is 1.1015g/kg to the 48h half lethal concentration of Cyprinus carpio, and safe concentration is 0.1102g/kg.
(3) compound Chinese Gall fishing antibacterial agent water preparation is stable
Stability is measured: adopting classical constant temperature method and the room temperature method that keeps sample that compound Chinese Gall antibacterial water preparation is carried out stability test, is standard substance with the gallic acid, and ultraviolet spectrophotometer 276nm wavelength is measured.Classical constant temperature method is by Arrhenius exponential law prediction antibacterial effect duration, and the result shows antibacterial room temperature speed constant K
25 ℃ 0.9=0.00189, the room temperature storage phase is t
25 ℃ 0.9=0.1054/K=1672.50h ≈ 70 days.The room temperature method that keeps sample is measured, when drawing 75 days in the antibacterial water preparation relative amount of gallic acid be 90.6%.
The specific embodiment
Embodiment 1:
Take by weighing 20 kilograms of Galla Chinensiss, 20 kilograms of Herba Andrographis, 15 kilograms of Radix Sophorae Flavescentiss, 15 kilograms of Cortex Phellodendris.With each component single medicinal material drying, pulverize the back and cross 100 mesh sieves, press formula proportion and mix, promptly make powder.
Embodiment 2:
Take by weighing Galla Chinensis 200g, Herba Andrographis 200g, Radix Sophorae Flavescentis 150g, Cortex Phellodendri 150g.After making powder, in W: V=1: it is 70% ethanol that 8 ratio adds concentration, with hydrochloric acid regulate pH value be 2,76 ℃ down with Soxhlet extraction apparatus reflux, extract, 90 minutes, collect liquid, residue repeats to extract twice again, merges extract, evaporating into does not have the alcohol flavor, adds water to 35000ml, promptly makes water preparation.
Claims (1)
1. a compound Chinese Gall fishing antibacterial agent is characterized in that, each component of this antibacterial and content are: 20 parts of Galla Chinensiss, 20 parts of Herba Andrographis, 15 parts of Radix Sophorae Flavescentiss, 15 parts of Cortex Phellodendris.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100951657A CN1939468B (en) | 2006-09-27 | 2006-09-27 | Compound Chinese Gall antiseptic prescription for fishing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100951657A CN1939468B (en) | 2006-09-27 | 2006-09-27 | Compound Chinese Gall antiseptic prescription for fishing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1939468A CN1939468A (en) | 2007-04-04 |
| CN1939468B true CN1939468B (en) | 2010-04-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006100951657A Expired - Fee Related CN1939468B (en) | 2006-09-27 | 2006-09-27 | Compound Chinese Gall antiseptic prescription for fishing |
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| Country | Link |
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| CN (1) | CN1939468B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8734867B2 (en) | 2007-12-28 | 2014-05-27 | Liveleaf, Inc. | Antibacterial having an extract of pomegranate combined with hydrogen peroxide |
| CA2754226C (en) * | 2009-03-04 | 2017-02-28 | Alexander L. Huang | Method and material for site activated complexing of biologic molecules |
| CN102021825B (en) * | 2010-11-24 | 2012-10-31 | 东华大学 | Method for preparing multifunctional antibacterial mothproof compound preparation of plant source for wool product |
| CN102225077B (en) * | 2011-06-23 | 2013-02-13 | 广西壮族自治区中国科学院广西植物研究所 | Application of Chinese gall extract for controlling streptococcal disease in aquaculture animals |
| CN102283908B (en) * | 2011-07-28 | 2013-04-03 | 浙江省淡水水产研究所 | Chinese and western medicinal compound preparation for preventing and treating bullfrog streptococcus agalactiae diseases and preparation method |
| US8716351B1 (en) | 2012-12-23 | 2014-05-06 | Liveleaf, Inc. | Methods of treating gastrointestinal spasms |
| CN103860663B (en) * | 2014-03-25 | 2016-05-25 | 中国科学院水生生物研究所 | A kind of control mycotic Chinese medicine preparation of aquiculture animal and preparation method |
| CN108783115A (en) * | 2018-07-12 | 2018-11-13 | 漳州傲农牧业科技有限公司 | A kind of immunopotentiator and the preparation method and application thereof preventing red head disease of Pseudobagrus fulvidraco |
Citations (2)
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| CN1131035A (en) * | 1995-12-05 | 1996-09-18 | 廖利平 | Chinese herbal medicine composition |
| CN1730089A (en) * | 2005-07-29 | 2006-02-08 | 陈福才 | Chinese traditional medicine with hemorrhoid resolving, poison expelling and stagnation expelling functions |
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- 2006-09-27 CN CN2006100951657A patent/CN1939468B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1131035A (en) * | 1995-12-05 | 1996-09-18 | 廖利平 | Chinese herbal medicine composition |
| CN1730089A (en) * | 2005-07-29 | 2006-02-08 | 陈福才 | Chinese traditional medicine with hemorrhoid resolving, poison expelling and stagnation expelling functions |
Non-Patent Citations (4)
| Title |
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| 姜蕾，等.五倍子对3种致病菌的体外抑菌和急性毒性试验.水利渔业25 6.2005,25(6),89-90，109. |
| 姜蕾，等.五倍子对3种致病菌的体外抑菌和急性毒性试验.水利渔业25 6.2005,25(6),89-90，109. * |
| 王海华.中草药防治水产动物疾病及药理学研究进展.中兽医学杂志 4.2004,(4),37-41. * |
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| CN1939468A (en) | 2007-04-04 |
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Granted publication date: 20100414 Termination date: 20120927 |