CN1899021A - Quick breeding method for root of kudzu vine - Google Patents
Quick breeding method for root of kudzu vine Download PDFInfo
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- CN1899021A CN1899021A CN 200510043016 CN200510043016A CN1899021A CN 1899021 A CN1899021 A CN 1899021A CN 200510043016 CN200510043016 CN 200510043016 CN 200510043016 A CN200510043016 A CN 200510043016A CN 1899021 A CN1899021 A CN 1899021A
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Abstract
The quick breeding method of kudzu vine includes the following steps: cutting kudzu vine with leaf into 5-8mm long bud section, soaking the bud section in breeding liquid for 1-2 hr, sealing the end cuts of bud section with the compounded cut treating material, setting the bud section in nursery bed with spread lower perlite grain layer of 3cm thickness and upper fine sand layer of 3cm thickness and covering arched shed, growing the bud section in the shed with controlled temperature 15-33deg.c, humidity of 70 % and illumination rate of 30 % to form complete regenerated plant within 20 days. The method is simple, fast in breeding speed, and suitable for fast breeding of kudzu vine.
Description
Technical field
The present invention relates to a kind of plant propagation renovation process, especially root of kudzu vine microbody material method for quickly breeding.
Background technology
The root of kudzu vine is that perennial liana belongs to leguminous plant, the root piece of Pueraria lobota, and medicine-food two-purpose, the cool flavor of gas levelling is sweet, and tool is well clearing heat and detoxicating, promotes the production of body fluid to quench thirst, tonifying Qi benefit stomach, diuresis antidiarrheal, activating spleen by relieving alcoholism etc. are all multi-functional.Can compare with American Ginseng because of its effect, traditional Chinese medical science famous expert is referred to as the good reputation of medicine food dual purpose plant " gold ", so the title of " gold Pueraria lobota " is arranged, health ministry is formally assert the root of kudzu vine as the mountain region plant, be food be again medicine.The root of kudzu vine becomes one of exploration project of tool wealth power of new century just day by day.And present 500,000 mu of China's root of kudzu vine cultivated area less thaies, market has openings is very big.
The breeding of the root of kudzu vine has traditional cuttage, press strip and method for tissue culture.Though traditional cuttage or press strip method are not high to its specification requirement, grasp easily and use,, its reproduction speed is slow, to maternal material requirements height, consumption is big, survival rate is low, also is subjected to the restriction in season; If do the breeding of the root of kudzu vine according to existing method for tissue culture, must in the laboratory, carry out so, need corresponding apparatus, the seedling quantity of cultivation is also limited, and not only cost is too high, and can't satisfy the wilderness demand of actual production.
Summary of the invention
The objective of the invention is to propose a kind of method that can breed the root of kudzu vine in big field industrial fast speed, its is not only grasped easily and uses, and the unification of the plant of breeding is complete, survival rate is high, also should not be subject to seasonal restrictions.
The technical scheme that realizes above-mentioned purpose is to carry out as follows:
(1) the grow seedlings bed and the preparation of seedling growth greenhouse:
Grow seedlings bed long 8-10 rice, wide 3-4 rice, excavate wide 0.3 meter at wide 1/2nd places of bed surface, one in dark 0.5 meter ditch, on the seedbed, spread the perlite particle of 3 centimeters of bed thickness earlier, again on the perlite upper berth 3 centimeters thick fine sand grains as the breeding bed surface, take little shed above this seedbed, the canopy face adopts thick 0.6mm agricultural film; After the bed surface of growing seedlings in the shed performs, to the environment in the canopy and the bed sterilization that carries out disinfection of growing seedlings, and a sand bed two shut, it is standby to shine the two ends of raising canopy after 48 hours with solar glare;
(2) preparation of propagating liquid:
Shine the grand 3000mg of benzene
Auxiliary agent DA-6 1800mg
Glucose 960mg
Antioxidant vitamin E 240mg
The preparation main points: according to after the ratio preparation, add content and be 95% medical alcohol, dosage is 100ml, adds the warm boiling water of 1000ml during use.
(3) preparation of root of kudzu vine otch material processed agent:
Cobastab
2, Cobastab
6, vitamin PP, starch, sterilization ling
Proportioning is respectively: 3: 4: 4: 88: 1
Preparation main points: stir, leave the shady and cool ventilation part in.
(4) female parent is bred the preparation of material:
Earlier full, the normotrophic band of intercepting 5-8mm leaf bud Ye Gegen branch is cut into 45 to 2mm place, the leaf bud of this material top again, this bud is saved to be immersed in the propagating liquid of above-mentioned (2) preparing 1-2 hour then;
(5) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 5-10 minute, the two ends otch to the bud joint seals processing with the otch material processed agent of above-mentioned (3) preparation again, then it is seeded on the husky bed according to spacing 5 * 5cm;
(6) postvaccinal management:
After bud joint is seeded in husky bed and goes up in 20 days, should control that temperature of shed is 70% at 15-33 ℃, humidity, the illumination rate is 30%.
According to the method described above, the bud joint just began to take root in the 4th day, just can form the whole plant of regenerating in 20 days.Effect intentionally of the present invention is:
(1) technology is simple, and reproduction speed is fast, the installations and facilities less investment, and cost is low, is easy to promote;
(2) save maternal propagating materials, no matter south, the north are throughout the year all to breed;
(3) carry out the quick propagation production of batch production in big field, reproduction coefficient becomes how much multiples to increase easily large-scale production;
(4) seedling of breeding fast, well developed root system, seedling is strong, and the survival rate height does not have variation.
Embodiment
Below in conjunction with embodiment the present invention is done description in further detail:
Embodiment 1
(1) the grow seedlings bed and the preparation of seedling growth greenhouse:
Long 8 meters, wide 4 meters bed of growing seedlings of arrangement on the level land, vertically one of excavation is wide 0.3 meter at wide 1/2nd places of bed surface, dark 0.5 meter ditch, on this seedbed, spread the perlite particle of a bed thickness 3cm earlier, again at the thick fine sand grain of perlite upper berth 3cm as the breeding bed surface, after the bed of growing seedlings is carried out, take a little shed with bamboo chip or steel pipe above it, the highest top of canopy is a 2.5-3 rice, the canopy face adopts thick 0.6mm film, land around the canopy face,, at last the film at husky bed two is hung down the sand bed is shut then with the bed surface disinfection.By solar light irradiation 48 hours, make the humidity 70% of husky bed, temperature reaches 50 ℃, and the two ends of raising canopy then are standby.
(2) preparation of propagating liquid:
Shine the grand 3000mg of benzene
Auxiliary agent DA-6 1800mg
Glucose 960mg
Antioxidant vitamin E 240mg
Above-mentioned material mixes according to quantity, and elder generation dissolves with 95% alcohol, adds water to 1000ml then.
(3) preparation of root of kudzu vine otch material processed agent:
Cobastab
2, Cobastab
6, vitamin PP, starch, sterilization ling
Proportioning is respectively: 3: 4: 4: 88: 1
Above-mentioned material mixes in proportion, and has deposited the shady and cool ventilation part.
(4) female parent is bred the preparation of material:
Full, the normotrophic band of vertical intercepting 6mm leaf bud Ye Gegen branch, branch after exsomatizing will in time keep the skin wet, again 2mm place in the leaf bud of this material top is cut into 45, below 3mm vertically cuts off at place, this bud is saved to be immersed in the propagating liquid of above-mentioned (2) preparing 1 to 2 hour then.
(5) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 10 minutes, the two ends otch to the bud joint seals it with the otch material processed agent of above-mentioned (3) preparation again, then the bud joint is seeded on the husky bed according to spacing 5 * 5cm.During inoculation, the lower end notch portion of bud joint is imbedded in the fine sand grain of breeding bed, and the leaf bud of parent and upper cut position are exposed.
(6) postvaccinal management:
When being seeded in, bud joint should control after husky bed is gone up that temperature of shed is 70% at 15-33 ℃, humidity, the illumination rate is 30%.The bud joint just began to take root in the 4th day, just can form the whole plant of regenerating in 20 days.
Embodiment 2
The grow seedlings bed and the preparation of seedling growth greenhouse:
Long 8 meters, wide 4 meters bed of growing seedlings of arrangement on the level land, vertically one of excavation is wide 0.3 meter at wide 1/2nd places of bed surface, and dark 0.5 meter ditch is taken a little shed with bamboo chip above it, and the highest top of canopy is a 2.5-3 rice, and the canopy face adopts thick 0.6mm film.
(2) preparation of propagating liquid:
Finish according to embodiment 1 described step;
(3) preparation of root of kudzu vine otch material processed agent:
Finish according to embodiment 1 described step;
(3) female parent is bred the preparation of material:
Finish according to embodiment 1 described step;
(4) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 10 minutes, the two ends otch to the bud joint seals it with the agent of otch material processed again, then the bud joint is seeded in the nutrient cup that nutrition soil is housed.
(5) postvaccinal management:
After bud joint is seeded in nutrient cup, nutrient cup is placed on the bed of growing seedlings according to spacing 5 * 5cm or 4 * 4cm, the control temperature of shed is 70% at 15-33 ℃, humidity, the illumination rate is 30%.
Claims (1)
1. quick breeding method for root of kudzu vine is characterized in that carrying out as follows:
(1) the grow seedlings bed and the preparation of seedling growth greenhouse:
Grow seedlings bed long 8-10 rice, wide 3-4 rice, excavate wide 0.3 meter at wide 1/2nd places of bed surface, one in dark 0.5 meter ditch, on the seedbed, spread the perlite particle of 3 centimeters of bed thickness earlier, again on the perlite upper berth 3 centimeters thick fine sand grains as the breeding bed surface, take little shed above this seedbed, the canopy face adopts thick 0.6mm agricultural film; After the bed surface of growing seedlings in the shed performs, to the environment in the canopy and the bed sterilization that carries out disinfection of growing seedlings, and a sand bed two shut, it is standby to shine the two ends of raising canopy after 48 hours with solar glare;
(2) preparation of propagating liquid:
Shine the grand 3000mg of benzene
Auxiliary agent DA-6 1800mg
Glucose 960mg
Antioxidant vitamin E 240mg
The preparation main points: according to after the ratio preparation, add content and be 95% medical alcohol, dosage is 100ml, adds the warm boiling water of 1000ml during use;
(3) preparation of root of kudzu vine otch material processed agent:
Cobastab
2, Cobastab
6, vitamin PP, starch, sterilization ling
Proportioning is respectively: 3: 4: 4: 88: 1
Preparation main points: stir, deposited the shady and cool ventilation part;
(4) female parent is bred the preparation of material:
Earlier full, the normotrophic band of intercepting 5-8mm leaf bud Ye Gegen branch is cut into 45 to 2mm place, the leaf bud of this material top again, this bud is saved to be immersed in the propagating liquid of above-mentioned (2) preparing 1-2 hour then;
(5) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 5-10 minute, the two ends otch to the bud joint seals processing with the otch material processed agent of above-mentioned (3) preparation again, then it is seeded on the husky bed according to spacing 5 * 5cm;
(6) postvaccinal management:
After bud joint is seeded in husky bed and goes up in 20 days, should control that temperature of shed is 70% at 15-33 ℃, humidity, the illumination rate is 30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510043016 CN1899021A (en) | 2005-07-20 | 2005-07-20 | Quick breeding method for root of kudzu vine |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200510043016 CN1899021A (en) | 2005-07-20 | 2005-07-20 | Quick breeding method for root of kudzu vine |
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| CN1899021A true CN1899021A (en) | 2007-01-24 |
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| CN 200510043016 Pending CN1899021A (en) | 2005-07-20 | 2005-07-20 | Quick breeding method for root of kudzu vine |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144555A (en) * | 2011-01-27 | 2011-08-10 | 中国科学院武汉植物园 | Rapid propagation method for radix puerariae |
| CN102783347A (en) * | 2012-08-28 | 2012-11-21 | 句容茅宝葛业有限公司 | Method for manually breeding kudzu roots quickly |
| CN103444406A (en) * | 2013-09-03 | 2013-12-18 | 夏华 | High-yield cultivation method for kudzu root |
| CN105340418A (en) * | 2015-11-06 | 2016-02-24 | 广西大海阳光药业有限公司 | Radix puerariae rapid breeding method |
| CN105379534A (en) * | 2015-11-19 | 2016-03-09 | 江苏天禄茶林有限公司 | High-quality and high-yield planting method for kudzuvine roots |
| CN106212025A (en) * | 2016-08-25 | 2016-12-14 | 绵阳市韵圣农业开发有限公司 | A kind of breeding method of Radix Puerariae Seedling |
| CN107114086A (en) * | 2017-03-31 | 2017-09-01 | 安徽胜华农业发展有限公司 | A kind of root of kudzu vine standardization cuttage and seedling culture method |
| WO2017166567A1 (en) * | 2016-04-02 | 2017-10-05 | 江苏辉丰农化股份有限公司 | Synergistic plant growth regulator composition |
| CN107242038A (en) * | 2017-06-20 | 2017-10-13 | 安徽胜华农业发展有限公司 | A kind of implantation methods for improving root of kudzu vine puerarin content |
| CN108575356A (en) * | 2018-04-02 | 2018-09-28 | 桂阳县经国中药材种植专业合作社 | A kind of pueraria lobata implantation methods |
-
2005
- 2005-07-20 CN CN 200510043016 patent/CN1899021A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144555A (en) * | 2011-01-27 | 2011-08-10 | 中国科学院武汉植物园 | Rapid propagation method for radix puerariae |
| CN102144555B (en) * | 2011-01-27 | 2012-08-22 | 中国科学院武汉植物园 | Rapid propagation method for radix puerariae |
| CN102783347A (en) * | 2012-08-28 | 2012-11-21 | 句容茅宝葛业有限公司 | Method for manually breeding kudzu roots quickly |
| CN103444406A (en) * | 2013-09-03 | 2013-12-18 | 夏华 | High-yield cultivation method for kudzu root |
| CN105340418A (en) * | 2015-11-06 | 2016-02-24 | 广西大海阳光药业有限公司 | Radix puerariae rapid breeding method |
| CN105379534A (en) * | 2015-11-19 | 2016-03-09 | 江苏天禄茶林有限公司 | High-quality and high-yield planting method for kudzuvine roots |
| WO2017166567A1 (en) * | 2016-04-02 | 2017-10-05 | 江苏辉丰农化股份有限公司 | Synergistic plant growth regulator composition |
| CN106212025A (en) * | 2016-08-25 | 2016-12-14 | 绵阳市韵圣农业开发有限公司 | A kind of breeding method of Radix Puerariae Seedling |
| CN107114086A (en) * | 2017-03-31 | 2017-09-01 | 安徽胜华农业发展有限公司 | A kind of root of kudzu vine standardization cuttage and seedling culture method |
| CN107242038A (en) * | 2017-06-20 | 2017-10-13 | 安徽胜华农业发展有限公司 | A kind of implantation methods for improving root of kudzu vine puerarin content |
| CN108575356A (en) * | 2018-04-02 | 2018-09-28 | 桂阳县经国中药材种植专业合作社 | A kind of pueraria lobata implantation methods |
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