CN1871019A - 一种可常温保存的生物羊膜的制备方法 - Google Patents
一种可常温保存的生物羊膜的制备方法 Download PDFInfo
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- CN1871019A CN1871019A CNA2004800239337A CN200480023933A CN1871019A CN 1871019 A CN1871019 A CN 1871019A CN A2004800239337 A CNA2004800239337 A CN A2004800239337A CN 200480023933 A CN200480023933 A CN 200480023933A CN 1871019 A CN1871019 A CN 1871019A
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- 238000002360 preparation method Methods 0.000 title claims description 17
- 210000001691 amnion Anatomy 0.000 claims abstract description 42
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 16
- 210000002826 placenta Anatomy 0.000 claims abstract description 9
- 235000011187 glycerol Nutrition 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000007710 freezing Methods 0.000 claims abstract description 3
- 230000008014 freezing Effects 0.000 claims abstract description 3
- 238000004806 packaging method and process Methods 0.000 claims abstract 2
- 210000003425 amniotic epithelial cell Anatomy 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 7
- 238000009833 condensation Methods 0.000 claims description 5
- 230000005494 condensation Effects 0.000 claims description 5
- 238000005057 refrigeration Methods 0.000 claims description 5
- 238000009461 vacuum packaging Methods 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 210000001136 chorion Anatomy 0.000 description 5
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- 208000006454 hepatitis Diseases 0.000 description 5
- 231100000283 hepatitis Toxicity 0.000 description 5
- 208000002672 hepatitis B Diseases 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 210000005059 placental tissue Anatomy 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 208000011580 syndromic disease Diseases 0.000 description 5
- 208000006379 syphilis Diseases 0.000 description 5
- 238000007689 inspection Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000405 serological effect Effects 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000012432 Collagen Type V Human genes 0.000 description 1
- 108010022514 Collagen Type V Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 1
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000002469 basement membrane Anatomy 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (7)
- 权 利 要 求 书1. 一种可常温保存的生物羊膜的制备方法, 其特征在于: 将胎盘的羊膜与絨 毛膜钝性剥离, 洗净, 得到的羊膜上皮细胞层在上, 经真空干燥使羊膜水 份含量范围在 1 %〜20%, 即得适宜在常温保存的生物羊膜。
- 2. 根据权利要求 1所述的可常温保存的生物羊膜的制备方法, 其特征在于: 羊膜真空干燥前进行了冷冻处理。
- 3. 根据权利要求 1或 2所述的可常温保存的生物羊膜的制备方法, 其特征在 于:洗净的羊膜经用 0. 1 %〜30%的甘油处理,置冷冻干燥机工作室经 0°C〜 一 56°C预冻 1〜10小时, 开启冷凝室制冷, 使冷阱温度达到一 10°C〜一 56 °C, 开启真空泵, 真空冷冻干燥, 使羊膜水份含量达到 1 %〜15 %, 再包 装、 灭菌, 即可。
- 4. 根据权利要求 3所述的可常温保存的生物羊膜的制备方法, 其特征在于: 冷冻干燥机工作室最佳温度范围: 0 °C 〜一 30°C,预冻最佳时间范围: 1一 2小时, 冷阱最佳温度范围: 一 30<sup>U</sup>C 〜一 45°C, 最佳真空度 2Pa〜15Pa, 真 空冷冻干燥最佳时间范围: 3〜10小时, 羊膜最佳含水量范围: 5 %〜12 %。
- 5. 根据权力要求 3所述的可常温保存的生物羊膜的制备方法, 其特征在于: 真空冷冻干燥时间 1〜72小时。
- 6. 根据权力要求 3所述的可常温保存的生物羊膜的制备方法, 其特征在于: 真空冷冻干燥时,真空度范围 lPa〜40Pa。
- 7. 根据权力要求 3所述的可常温保存的生物羊膜的制备方法, 其特征在于: 包装, 采用真空包装。
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB031508383A CN1241474C (zh) | 2003-09-03 | 2003-09-03 | 生物羊膜保存处理方法 |
CN03150838.3 | 2003-09-03 | ||
CN200410075361.9 | 2004-08-27 | ||
PCT/CN2004/001010 WO2005021014A1 (fr) | 2003-09-03 | 2004-09-02 | Preparation d'un amnios biologique conservable a temperature ambiante |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1871019A true CN1871019A (zh) | 2006-11-29 |
CN100496238C CN100496238C (zh) | 2009-06-10 |
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Application Number | Title | Priority Date | Filing Date |
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CNB031508383A Expired - Lifetime CN1241474C (zh) | 2003-09-03 | 2003-09-03 | 生物羊膜保存处理方法 |
CNB2004800239337A Active CN100496238C (zh) | 2003-09-03 | 2004-09-02 | 一种可常温保存的生物羊膜的制备方法 |
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Application Number | Title | Priority Date | Filing Date |
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CNB031508383A Expired - Lifetime CN1241474C (zh) | 2003-09-03 | 2003-09-03 | 生物羊膜保存处理方法 |
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CN (2) | CN1241474C (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103874762A (zh) * | 2011-08-26 | 2014-06-18 | 组织技术公司 | 胎儿支持组织的灭菌方法 |
US9808491B2 (en) | 2014-06-03 | 2017-11-07 | Tissuetech, Inc. | Compositions of morselized umbilical cord and/or amniotic membrane and methods of use thereof |
US11590265B2 (en) | 2015-02-23 | 2023-02-28 | Biotissue Holdings Inc. | Apparatuses and methods for treating ophthalmic diseases and disorders |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1311072C (zh) * | 2005-07-12 | 2007-04-18 | 何伟 | 干燥活性羊膜的制备方法 |
EP1944045B1 (en) * | 2005-07-25 | 2013-09-18 | Foundation for Biomedical Research and Innovation | Sheet-like composition |
CN101889569B (zh) * | 2010-07-23 | 2013-05-22 | 马吉献 | 一种生物羊膜干燥保存方法 |
CN102631707A (zh) * | 2012-05-04 | 2012-08-15 | 厦门大学 | 一种基于羊膜的生物材料及其制备方法与用途 |
CN104825493B (zh) * | 2015-05-06 | 2020-02-21 | 广州瑞泰生物科技有限公司 | 一种用于眼表治疗的生物羊膜及其制备方法 |
CN104826166B (zh) * | 2015-05-06 | 2017-06-06 | 广州优适清生物科技有限公司 | 一种用于青光眼治疗的生物膜及其制备方法 |
CN105028386A (zh) * | 2015-06-05 | 2015-11-11 | 热孜万·买买提明 | 活性羊膜保存方法 |
CN107296041B (zh) * | 2017-07-02 | 2020-12-25 | 江西瑞济生物工程技术股份有限公司 | 一种新鲜羊膜保存液及新鲜羊膜保存方法与应用 |
EP3655000A4 (en) * | 2017-07-11 | 2021-07-14 | Universal Stabilization Technologies, Inc. | PROCESS FOR THE PRESERVATION OF BIOPHARMACEUTICALS |
CN109288867A (zh) * | 2018-10-26 | 2019-02-01 | 上海慧存医疗科技有限公司 | 一种复合羊膜粉制剂的制备方法 |
-
2003
- 2003-09-03 CN CNB031508383A patent/CN1241474C/zh not_active Expired - Lifetime
-
2004
- 2004-09-02 CN CNB2004800239337A patent/CN100496238C/zh active Active
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103874762A (zh) * | 2011-08-26 | 2014-06-18 | 组织技术公司 | 胎儿支持组织的灭菌方法 |
US9682160B2 (en) | 2011-08-26 | 2017-06-20 | Tissuetech, Inc. | Methods of sterilizing fetal support tissues |
US9931423B2 (en) | 2011-08-26 | 2018-04-03 | Tissuetech, Inc. | Methods of sterilizing fetal support tissues |
US9808491B2 (en) | 2014-06-03 | 2017-11-07 | Tissuetech, Inc. | Compositions of morselized umbilical cord and/or amniotic membrane and methods of use thereof |
US11116800B2 (en) | 2014-06-03 | 2021-09-14 | Tissuetech, Inc. | Compositions of morselized umbilical cord and/or amniotic membrane and methods of use thereof |
US11590265B2 (en) | 2015-02-23 | 2023-02-28 | Biotissue Holdings Inc. | Apparatuses and methods for treating ophthalmic diseases and disorders |
Also Published As
Publication number | Publication date |
---|---|
CN100496238C (zh) | 2009-06-10 |
CN1241474C (zh) | 2006-02-15 |
CN1522568A (zh) | 2004-08-25 |
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Owner name: JIANGXI RUIJI BIO-ENGINEERING TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: MIAO JIUCHANG Effective date: 20131028 |
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Effective date of registration: 20131028 Address after: 330029 No. 382 Fang Fang Road, Nanchang, Jiangxi Patentee after: Jiangxi Ruiji Bio-Engineering Technology Co., Ltd. Address before: 330029 No. 108 Fang Fang Road, Nanchang, Jiangxi Patentee before: Miao Jiuchang |
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Address after: 330096 Nanchang province high tech Development Zone, Jiangxi Road, No. 498 Patentee after: JIANGXI RUIJI BIO-ENGINEERING TECHNOLOGY CO., LTD. Address before: 330029 No. 382 Fang Fang Road, Nanchang, Jiangxi Patentee before: Jiangxi Ruiji Bio-Engineering Technology Co., Ltd. |